KNAUER Eurospher II 100 Diol Prep HPLC Columns (USP L20)

Diol columns for Normal Phase in HPLC and UHPLC (USP L20)

Diol packing material can be universally used in different polar modes (NP, RP and HILIC). Diol bonded phases are a versatile alternative to unbonded silica for normal phase separations with shorter equilibration times.

The hydroxyl groups provide good selectivity without excessive retention, caused by the weaker H-bonding of the diol layer than of silanols. Some diol bonded phases have been developed specifically for applications in the HILIC mode.

Properties

• Separation mechanism: Hydrophilic interaction
• Ultra pure, spherical high performance HPLC phase based on silica gel
• Diol, no endcapping, with 5 % carbon content 

Key features

• High-class HPLC phase perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks, classical Diol phase without endcapping and 5 % carbon content, outstanding mechanical and chemical stability.

Recommended application areas

• Alternative to the silica packing with shorter equilibration time and comparable selectivity; due to the lower activity of these packings it can be also used for SEC-applications.

KNAUER: Diol

Tip: The most important column parameters for evaluation are retention time, peak symmetry and theoretical plates. Retention time to prove the reproducibility of
measurements. Peak symmetry to estimate how good the column bed is packed (peak symmetry value of 1.0 is optimal). Theoretical plate numbers to determine the effectiveness of the HPLC column.

Stationary phase Eurospher II 100 Diol is provided in these column types: analytical, semi-preparative and preparative.

Eurospher II 100 Diol Preparative Standard Columns are available with these parameters:

• Column length (mm): 50, 150, 250
• Column ID (mm): 20, 30, 50
• Particle size (µm): 5, 10, 15

Eurospher II 100 Diol Preparative dAX Columns with dynamic axial compression are available with these parameters:

• Column length (mm): 50, 150, 250
• Column ID (mm): 25, 30, 50
• Particle size (µm): 5, 10, 15

KNAUER Preparative HPLC Columns

The right choice for all dimensions from semi-prep to batch chromatography to SMB.

• Modifications: Reversed Phase, HILIC, Normale Phase
• Max. Pressure: 400–600 bar
• Column Inner Diameter (ID): 16–50 mm in our shop, more on request
• Column Brands: Eurospher, Eurospher II
• Safe Investment: Robustness and durability based on superior technology
• Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.
• Customizable: Standard flanged hardware & dAX hardware with static or dynamic axial compression available

KNAUER: KNAUER Preparative HPLC Columns

Preparative HPLC columns can be offered with two different hardware types:

• Versatile standard flanged hardware for nearly any application task in batch chromatography
• dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes