KNAUER Eurospher II 100 Si Prep HPLC Columns for NP & HILIC (USP L3)

Si columns for Normal Phase and HILIC applications (USP L3)

Unmodified Silica gel materials were the first ones used for chromatography. This is the reason the mode is called "normal phase" (NP). They are still used in HPLC, although reversed phase (RP) is by far the most often used HPLC mode today.

A wide range of porous Si materials are available, characterized by:

• Surface area
• Pore size
• Particle size
• Purity
• Metal content

Properties

• Separation mechanism: Hydrophilic interactions
• Ultra pure, spherical high performance HPLC phase based on silica gel
• No modification, no endcapping, with 0 % carbon content

Key features

• High-class HPLC phase perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks, outstanding mechanical and chemical stability.

Recommended application areas

• Wide range of different applications, i.e. SEC (size exclusion chromatography) but also for normal phase HPLC and HILIC; good choice for analytical and preparative purposes to separate polar compounds.

Tip: Normal phase (NP) stationary phases have been the first phases for chromatography and that is why their properties of polarity were determined as ’’normal’’. The stationary phase of a NP column has polar properties and is commonly used with nonpolar solvents as hexane or heptane. The other way round a reversed phase (RP) column has covalent charcteristics and is used with more polar eluents, like mixtures of acetonitrile and water.

Stationary phase Eurospher II 100 Si is provided in these column types: analytical, semi-preparative and preparative.

Eurospher II 100 Si Preparative Standard Columns are available with these parameters:

• Column length (mm): 50, 150, 250
• Column ID (mm): 20, 30, 50
• Particle size (µm): 5, 10, 15

Eurospher II 100 Si Preparative dAX Columns with dynamic axial compression are available with these parameters:

• Column length (mm): 50, 150, 250
• Column ID (mm): 25, 30, 50
• Particle size (µm): 5, 10, 15

KNAUER Preparative HPLC Columns

The right choice for all dimensions from semi-prep to batch chromatography to SMB.

• Modifications: Reversed Phase, HILIC, Normale Phase
• Max. Pressure: 400–600 bar
• Column Inner Diameter (ID): 16–50 mm in our shop, more on request
• Column Brands: Eurospher, Eurospher II
• Safe Investment: Robustness and durability based on superior technology
• Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.
• Customizable: Standard flanged hardware & dAX hardware with static or dynamic axial compression available

KNAUER: KNAUER Preparative HPLC Columns

Preparative HPLC columns can be offered with two different hardware types:

• Versatile standard flanged hardware for nearly any application task in batch chromatography
• dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes