Waters XBridge BEH C18 HPLC Columns

As a workhorse for HPLC method development, XBridge BEH C18 sorbent can be used over an entire range of mobile phase pH (1-12). The trifunctionally bonded C18 ligand provides you with a universal column for most HPLC separations and gives you the widest usable pH range, superior low pH stability, and ultra-low column bleed. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.

Waters: XBridge BEH C18 HPLC Column

Specifications

• Chemistry: C18
• Separation Mode: Reversed Phase
• Particle Substrate: Hybrid
• pH Range Min: 1 pH
• pH Range Max: 12 pH
• Maximum Pressure: 10000 psi (690 Bar)
• Endcapped: Yes
• Silanol Activity: Low
• Particle Shape: Spherical
• System: UHPLC, UPLC
• Particle Technology: BEH
• USP Classification: L1
• Carbon Load: 18 %

The Waters XBridge BEH C18 HPLC Column is also available in these formats:

Waters XBridge BEH C18 Column with eConnect Column Tag

• This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS System running Empower software.

Waters: XBridge BEH C18 Column with eConnect Column Tag

Waters XBridge BEH Shield RP18 Column

• XBridge BEH Shield RP18 columns complement the XBridge BEH C18 and C8 phases and provide a necessary and powerful tool for HPLC method development. The embedded carbamate group in the bonded phase ligand provides alternate selectivity, especially for phenolic compounds compared to straight chain alkyl columns. 
• pH Range Min: 1 pH
• pH Range Max: 12 pH
• Bonding Technology: Shield RP18
• Carbon Load: 17 %

Waters XBridge BEH Shield RP18 Column with eConnect Column Tag

• This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS System running Empower software.

Waters XBridge Peptide BEH C18 XP Column

• The XBridge Peptide BEH C18 XP Columns are optimized and QC tested for peptide separations. The small-pore (130Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed for proteomic, peptide mapping, and synthetic peptide applications. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) to before being QC accepted as a Peptide separation column.
• Suggested Use: Peptide Separations, Peptide Analysis

XBridge Columns

Particle stability and performance for methods development

Withstanding extreme operating conditions like high pH and high temperatures is essential for methods development. XBridge HPLC and UHPLC Columns utilize Ethylene Bridged Hybrid (BEH) Particle Technology and are built to withstand high pH, high temperatures, and other extreme operating conditions like volatile eluents, for a wide range of small molecule and biomolecule LC-MS analysis.

XBridge HPLC and UHPLC Columns are available in a broad range of sorbent particles and sizes: XBridge eXtended Perfomance (XP) 2.5 µm Columns for rapid analytical UHPLC separations; 3.5 and 5 µm HPLC columns for reliable analytical method development; and 5 and 10 µm optimum bed density (OBD) preparative columns for purification chromatography.

Waters: XBridge Columns