Waters Atlantis Silica HILIC HPLC Columns

Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches.

Due to their hydrophilic nature combined with their net positive charge at acidic pH, retention and separation of very basic polar analytes are extremely difficult using reversed-phase chromatography. The HILIC technology is able to resolve these challenging separation problems. This is what makes the Atlantis Silica HILIC Column the ideal lab equipment for use in metabolism, drug discovery, and combinatorial chemistries.

Waters: Atlantis Silica HILIC HPLC Columns

Specifications

• Chemistry: HILIC
• Separation Mode: Hydrophilic Interaction (HILIC)
• Particle Substrate: Silica
• pH Range Min: 1 pH
• pH Range Max: 5 pH
• Maximum Pressure: 6000 psi (415 Bar)
• Endcapped: No
• Silanol Activity: High
• Particle Shape: Spherical
• Pore Size: 100 Å
• System: HPLC
• USP Classification: L3

The Waters Atlantis Silica HILIC HPLC Column is also available as:

Waters Atlantis Silica HILIC HPLC Column with eConnect Column Tag

• This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS System running Empower software.

Waters: Atlantis Silica HILIC HPLC Column with eConnect Column Tag

Atlantis Columns

Reversed-phase and HILIC HPLC Columns for superior polar compound retention

Polar analytes are difficult to retain using traditional reversed-phase (RP) chromatography as they often elute in the void volume of the column. You can solve this challenge today with Atlantis Columns, which retain and separate polar analytes under reversed phase and HILIC conditions and provide quick, accurate, and reproducible quantification with increased retention and sensitivity.

Available in T3, dC18, and the unbonded HILIC chemistries, the Waters Atlantis Column family provides exceptional performance, versatility, retention, and chromatographic stability for polar compounds and balanced retention for broad analyte mixtures. 

Overview

• Retains and separates small, water-soluble polar organic compounds
• Compatible with 100% aqueous mobile phases
• Improves detection limits, method reproducibility, and robustness by eliminating ion-pairing reagents
• Long column lifetimes using low-pH mobile phases
• Fully scalable in 3, 5, and 10 μm particle sizes
• Recommended Use: For the reproducible retention and separation of a broad range of polar and non-polar compounds.

Waters: Atlantis HPLC Columns