33 1 3048 7200In reversed-phase chromatography, polar compounds are eluted first, causing routine and complex separation challenges. With convergence chromatography, however, polar compounds are retained and eluted last - providing the separation power of normal-phase liquid chromatography (LC) with the ease-of-use of reversed-phase LC.
Using green and non-toxic compressed liquid CO₂ as a primary mobile phase, the ACQUITY UPC2® System delivers the ability to precisely vary mobile phase strength, pressure, and temperature. The ability to fine-tune the resolving power and selectivity of the system enables better control over the retention of analytes for separating, detecting, and quantifying structural analogs, isomers, and enantiomeric and diastereomeric mixtures – all compounds that are often a challenge to separate by any other means.
To solve routine and complex separations challenges that require unparalleled selectivity and ease-of-use.
Waters ACQUITY UPC2 System: ACQUITY UPC2 System gives scientists the ability to precisely vary mobile phase strength, pressure, and temperature.
By providing the ability to precisely vary mobile phase strength, pressure, and temperature, the Waters ACQUITY UPC2 System gives scientists the power to better control the retention of analytes for separating, detecting, and quantifying structural analogs, isomers, and enantiomeric and diastereomeric mixtures.
Waters ACQUITY UPC2 System: Separation of propranolol and hydroxy metabolites. Note that each hydroxy metabolite has an R and S form.
Unique in its capabilities, the pioneering ACQUITY UPC2 System delivers what the rest of the industry never could – a system, based on the principles of normal-phase LC, with the ease-of-use of reversed-phase LC, and purposefully built for analytical performance, placing convergence chromatography atop the list of separations tools in the kit of all analytical chemists.
Waters ACQUITY UPC2 System: UPC2 analysis can be 30x faster and use 75x less solvent per run - all with 100x lower cost per analysis.
With its separation potential for a far broader chemical spectrum of applications than that of reversed-phase LC-based instrumentation, the ACQUITY UPC2 System leverages convergence chromatography to enable superior performance, using inexpensive, compressed CO₂ as a non-toxic mobile-phase.
Waters ACQUITY UPC2 System: The ACQUITY UPC2-MS FFA analysis provides a simple and fast method with a significant reduction in analysis time compared to alternative techniques such as GC-MS, which requires FAME derivatization.
Harnessing what's now known as convergence chromatography, an evolution of the traditional SFC technique, the ACQUITY UPC2 System has the power to create selective separations of structurally similar chiral and achiral compounds.
The miscibility of CO₂ with a wide range of polar and non-polar organic solvents has made the liquid CO₂-based mobile phase versatile enough to separate a much wider range of compounds than reversed-phase LC, especially for mixtures containing polar compounds. Not only can CO₂-based solvents be used with both polar and non-polar stationary phases, but chromatography can be influenced by modulating solvent gradients with a much wider choice of columns using the same mass spectrometry-compatible co-solvents.
Being orthogonal to reversed-phase LC, UPC2 separations often show an inverse order of elution among groups of analytes. In combination with various detection techniques, this orthogonality is valuable for confirming analyte identity in complex matrices.
Waters ACQUITY UPC2 System: With ACQUITY UPC2 System, orthogonality to the most commonly used high/low pH approach in reversed-phase can be clearly seen as a useful addition to the separation challenges faced by synthesis analysis.
Waters ACQUITY UPC2 System: ACQUITY UPC2 System with ACQUITY QDa Detector.
Waters ACQUITY UPC2 System: ACQUITY UPC2 System with Xevo TQ-XS.
Waters ACQUITY UPC2 System: ACQUITY UPC2 System with ACQUITY UPLC ELS, ACQUITY UPC2 PDA, and ACQUITY QDa Detectors.
Waters ACQUITY UPC2 System: ACQUITY UPC2 System with Xevo G2-XS QTof.
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