Meeting EU Drinking Water Standards: The Critical Role of Sample Prep in Trace Contaminant Analysis

<h3>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</h3>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711">Among the analytical techniques available today, anion exchange chromatography (AEX) stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</a></blockquote><h4>How Scientists Read Oligonucleotides Using Charge</h4>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.<h4>Why Ion Exchange Works: Binding, Retention, and Release</h4>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, anion exchange is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.The system consists of two main components:<ul><li>Stationary phase: A positively charged resin that binds anionic analytes.</li><li>Mobile phase: An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"><h4>The Five-Step Workflow of Ion Exchange Chromatography</h4>AEX separations generally follow a well-established sequence of steps:<ol><li>Column equilibration The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li>Sample loading Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li>Washing The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li>Elution Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li>Regeneration and re-equilibration A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2 Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"><h4>Why AEX Is Especially Effective for Oligonucleotides</h4>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.Key factors influencing AEX behavior include:<ul><li>Charge density: Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li>Resin chemistry: Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li>Buffer composition: The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"><h4>AEX as the Gold Standard for Oligonucleotide Separation</h4>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:<ul><li>High resolving power: Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li>Predictable retention behavior: In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li>Compatibility with modified oligonucleotides: Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li>Excellent control via gradient elution: Salt gradients provide precise tuning of selectivity and resolution.</li></ul>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"><h4>Final Perspective: Mastering AEX with Confidence</h4>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.<blockquote>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at sales@knauer.net. More insights into practical oligonucleotide analysis will follow in our “Oligos Made Easy” series.For technical questions or in-depth discussion, the author can be reached at kindler@knauer.net</blockquote>

Oligos Made Easy - Part 2: AEX

Although modern agriculture needs to be based on accurate real-time data on soil, plant, and environmental conditions, affordable, sufficiently sensitive, and robust monitoring devices for this purpose are currently missing. According to scientists, low-cost sensors based on new smart materials and printing technologies offer a suitable solution. The results of multidisciplinary research thus far and proposals for the development of these devices have been presented by researchers from Czechia, Great Britain, Spain, and the USA in a review article in the prestigious journal <a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-68778-6#Abs1">Nature Communications</a>.“Sensor technologies for monitoring plant health are still in their infancy. Only simple sensors capable of monitoring basic parameters such as pH, soil moisture, or temperature are commonly available on the market. However, if farmers are to face current challenges, including climate change, and ensure sufficient food for humanity, they need much more effective tools that will enable them to monitor, for example, various pathogens, including bacteria and fungi. In our opinion, a suitable solution is a combination of new smart materials and printing for the production of inexpensive, durable, and scalable materials,” said the article’s first author, David Panáček from CATRIN, who worked on the study during his internship at Imperial College London.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg" alt="CATRIN: David Panáček" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg 208w," sizes="100vw" width="1536" height="1152">Sensors for monitoring plant health, for example, lag behind similar devices used in human medicine. According to the authors, advances in materials science, which will enable the development of scalable sensor technologies, play a key role in this potential shift. “Printing techniques, including inkjet printing, screen printing, aerosol printing, 3D printing, and direct laser writing, offer flexible ways to produce flexible sensors, large-area sensors, and sensors integrated directly into plants. The review article summarizes recent advances in printable low-dimensional materials for agricultural sensors, analyzes their physico-chemical properties in relation to sensor performance, and discusses key challenges and future opportunities requiring interdisciplinary integration,” said one of the corresponding authors, Michal Otyepka.The research brought together materials scientists, chemists, biologists, sensing experts, and printing technology specialists. Materials research and the use of graphene-based smart materials were led by scientists from CATRIN at Palacký University and VŠB-Technical University of Ostrava. Plant biologists from CATRIN contributed their knowledge of plant development, their responses to stress conditions, and the monitoring of their properties. Researchers from Imperial College London took care of the electronics, while representatives from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/36"> CEITEC – VUT</a> focused on printing technologies. The team also included leading experts in sensing from the Catalan Institute of Nanoscience and Nanotechnology in Barcelona, and Joseph Wang of the University of California San Diego, the “father of sensing and electrochemistry,” also contributed his extensive knowledge.“This is a groundbreaking study on this topic, which has managed to attract some truly big names in their respective fields. Last but not least, the study demonstrates that interdisciplinarity at CATRIN gives rise to completely new and promising research topics, such as plant sensing and its use for studying plant-environment interactions and smart agriculture,” added another corresponding author, Nuria De Diego.

Current sensors are not good enough. Scientists propose a new way to monitor plant health

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The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer

Orbitrap Fusion Tribrid MS combines quadrupole, ion trap, and Orbitrap analyzers for ultra-flexible MSⁿ workflows, enabling deep proteomics, PTM analysis, and confident structural elucidation.

<h4>Revolutionary tribrid architecture for comprehensive structural analysis and deep proteome coverage</h4>The Orbitrap Fusion Tribrid mass spectrometer combines a quadrupole, a linear ion trap, and an Orbitrap mass analyzer in a unique architecture that delivers unmatched analytical performance. It provides life scientists with advanced MSn capabilities, flexibility in fragmentation techniques, and exceptional resolution and speed — ideal for tackling the most complex analytical challenges in proteomics, PTMs, metabolomics, lipidomics, and small molecule research.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Fusion_Tribrid_hmotnostni_spektrometr_e93c577ae5/Pragolab-Thermo-Orbitrap-Fusion-Tribrid-hmotnostni-spektrometr.webp" alt="Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Fusion_Tribrid_hmotnostni_spektrometr_e93c577ae5/thumbnail_Pragolab-Thermo-Orbitrap-Fusion-Tribrid-hmotnostni-spektrometr.webp 245w," sizes="100vw" width="1914" height="1059"><h3>Key Features and Benefits</h3><h4>🔬 Revolutionary Tribrid Design</h4><ul><li>Combines quadrupole, linear ion trap, and Orbitrap analyzers</li><li>Perform MSⁿ up to n = 10 with detection in either ion trap or Orbitrap</li><li>Fragmentation options: CID, HCD, ETD, and EThcD at any MS stage</li><li>Synchronous Precursor Selection (SPS): up to 20 precursors per scan for MS³</li></ul><h4>⚙️ High Performance &amp; Resolution</h4><ul><li>Resolving power up to 500,000 FWHM at m/z 200</li><li>&lt;1 ppm mass accuracy with EASY-IC™ internal calibration</li><li>Polarity switching in 1.1 s full cycle</li><li>Scan speeds up to 20 Hz for Orbitrap and ion trap MSn</li></ul><h4>🧪 Versatility in Applications</h4><ul><li>Analyze low-abundance, high-complexity, or structurally complex samples</li><li>Confidently resolve isobaric interferences and detect PTMs</li><li>Full compatibility with intact protein analysis, glycoproteomics, and TMT SPS-MS³ quantitation workflows</li></ul><h4>🔁 Efficient Ion Handling</h4><ul><li>EASY-Max NG or NanoSpray Flex NG ion sources</li><li>Active Beam Guide and S-Lens for efficient ion transmission</li><li>Dynamic Scan Management optimizes scan scheduling and parallelization</li></ul><h4>💡 Ease of Use</h4><ul><li>Intuitive Tune and Method Editors</li><li>Method templates for routine and advanced workflows</li><li>Quanfirmation: HRAM SIM for quantitation + ion trap MS/MS for confirmation</li><li>Automated calibration, AGC, and exclusion logic</li></ul><h4>🧬 Compatible Software</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758">Xcalibur™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782">Proteome Discoverer™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1756">ProSight™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1757">Mass Frontier™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Compound Discoverer™</a>, and others</li><li>Support for data-dependent, targeted, and deconvolution workflows</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Fusion_Tribrid_hmotnostni_spektrometr_2_3d7accb56e/Pragolab-Thermo-Orbitrap-Fusion-Tribrid-hmotnostni-spektrometr-2.webp" alt="Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Fusion_Tribrid_hmotnostni_spektrometr_2_3d7accb56e/thumbnail_Pragolab-Thermo-Orbitrap-Fusion-Tribrid-hmotnostni-spektrometr-2.webp 245w," sizes="100vw" width="1038" height="622">

Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer

The Orbitrap Fusion Lumos Tribrid MS combines quadrupole, ion trap, and Orbitrap analyzers for high-resolution, ultra-sensitive MSn workflows in proteomics, metabolomics, and biopharma.

<h4>The most powerful tribrid MS platform for deep, multidimensional analysis in proteomics, metabolomics, and structural biology</h4>The Orbitrap Fusion Lumos Tribrid MS is a cutting-edge mass spectrometer that combines a quadrupole, a linear ion trap, and an Orbitrap™ high-resolution accurate-mass analyzer in a single platform. With unmatched sensitivity, selectivity, and versatility, it enables scientists to tackle complex biological questions with confidence—whether in discovery proteomics, targeted quantitation, top-down protein analysis, glycoproteomics, lipidomics, or small molecule research.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Fusion_Lumos_Tribrid_hmotnostni_spektrometr_1_39be5478b4/Pragolab-Thermo-Orbitrap-Fusion-Lumos-Tribrid-hmotnostni-spektrometr-1.webp" alt="Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Fusion_Lumos_Tribrid_hmotnostni_spektrometr_1_39be5478b4/thumbnail_Pragolab-Thermo-Orbitrap-Fusion-Lumos-Tribrid-hmotnostni-spektrometr-1.webp 245w," sizes="100vw" width="1823" height="1068"><h3>🔬 Unmatched Analytical Performance</h3><ul><li>&gt;500,000 resolution with Orbitrap Ultra-High Field analyzer</li><li>Sub-ppm mass accuracy with real-time calibration</li><li>Attomole-level quantification using PRM and DIA</li><li>High sensitivity MS/MS with HCD, CID, ETD, EThcD, and new ETD HD fragmentation</li></ul><h3>🔄 Flexibility Across Workflows</h3><ul><li>Tribrid architecture: combine quadrupole precursor selection, ion trap MSn, and Orbitrap detection</li><li>DIA, PRM, and SPS-MS3 quantification workflows with isobaric tags (e.g., TMT, TMTpro)</li><li>Top-down intact protein analysis with high sequence coverage</li><li>Fast polarity switching and intelligent acquisition for lipidomics</li></ul><h3>🧪 Ideal for Multi-Omics and PTMs</h3><ul><li>Deep profiling of glycopeptides, phosphoproteins, and other post-translational modifications</li><li>Resolution of isobaric isoforms, methylation patterns, and histone modifications</li><li>Confident structure elucidation of small molecules with fine isotope resolution</li></ul><h3>💡 Smart Technology for Productivity</h3><ul><li>Advanced Quadrupole Technology (AQT) for high transmission and selectivity</li><li>Automatic gain control (AGC) for optimal ion population</li><li>Easy ETD™ source and vent-free maintenance with source plug</li><li>Method templates and visual method editor for intuitive setup</li></ul><h3>🧬 Applications</h3><ul><li>Quantitative and discovery proteomics (e.g., large-scale TMT SPS-MS3)</li><li>Structural characterization of antibodies and proteoforms</li><li>Targeted metabolite quantitation and small molecule identification</li><li>Comprehensive glycoproteomics and lipidomics workflows</li></ul><h3>🧷 Software Compatibility</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758">Xcalibur™</a> and Tune for control and calibration</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782">Proteome Discoverer™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1756">ProSight™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/77">TraceFinder™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1757">Mass Frontier™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Compound Discoverer™</a> for data analysis across omics</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Fusion_Lumos_Tribrid_hmotnostni_spektrometr_3_7458a4eb26/Pragolab-Thermo-Orbitrap-Fusion-Lumos-Tribrid-hmotnostni-spektrometr-3.webp" alt="Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Fusion_Lumos_Tribrid_hmotnostni_spektrometr_3_7458a4eb26/thumbnail_Pragolab-Thermo-Orbitrap-Fusion-Lumos-Tribrid-hmotnostni-spektrometr-3.webp 245w," sizes="100vw" width="2578" height="1296">

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Oligos Made Easy - Part 2: AEX