Meeting EU Drinking Water Standards: The Critical Role of Sample Prep in Trace Contaminant Analysis

Although modern agriculture needs to be based on accurate real-time data on soil, plant, and environmental conditions, affordable, sufficiently sensitive, and robust monitoring devices for this purpose are currently missing. According to scientists, low-cost sensors based on new smart materials and printing technologies offer a suitable solution. The results of multidisciplinary research thus far and proposals for the development of these devices have been presented by researchers from Czechia, Great Britain, Spain, and the USA in a review article in the prestigious journal <a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-68778-6#Abs1">Nature Communications</a>.“Sensor technologies for monitoring plant health are still in their infancy. Only simple sensors capable of monitoring basic parameters such as pH, soil moisture, or temperature are commonly available on the market. However, if farmers are to face current challenges, including climate change, and ensure sufficient food for humanity, they need much more effective tools that will enable them to monitor, for example, various pathogens, including bacteria and fungi. In our opinion, a suitable solution is a combination of new smart materials and printing for the production of inexpensive, durable, and scalable materials,” said the article’s first author, David Panáček from CATRIN, who worked on the study during his internship at Imperial College London.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg" alt="CATRIN: David Panáček" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg 208w," sizes="100vw" width="1536" height="1152">Sensors for monitoring plant health, for example, lag behind similar devices used in human medicine. According to the authors, advances in materials science, which will enable the development of scalable sensor technologies, play a key role in this potential shift. “Printing techniques, including inkjet printing, screen printing, aerosol printing, 3D printing, and direct laser writing, offer flexible ways to produce flexible sensors, large-area sensors, and sensors integrated directly into plants. The review article summarizes recent advances in printable low-dimensional materials for agricultural sensors, analyzes their physico-chemical properties in relation to sensor performance, and discusses key challenges and future opportunities requiring interdisciplinary integration,” said one of the corresponding authors, Michal Otyepka.The research brought together materials scientists, chemists, biologists, sensing experts, and printing technology specialists. Materials research and the use of graphene-based smart materials were led by scientists from CATRIN at Palacký University and VŠB-Technical University of Ostrava. Plant biologists from CATRIN contributed their knowledge of plant development, their responses to stress conditions, and the monitoring of their properties. Researchers from Imperial College London took care of the electronics, while representatives from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/36"> CEITEC – VUT</a> focused on printing technologies. The team also included leading experts in sensing from the Catalan Institute of Nanoscience and Nanotechnology in Barcelona, and Joseph Wang of the University of California San Diego, the “father of sensing and electrochemistry,” also contributed his extensive knowledge.“This is a groundbreaking study on this topic, which has managed to attract some truly big names in their respective fields. Last but not least, the study demonstrates that interdisciplinarity at CATRIN gives rise to completely new and promising research topics, such as plant sensing and its use for studying plant-environment interactions and smart agriculture,” added another corresponding author, Nuria De Diego.

Current sensors are not good enough. Scientists propose a new way to monitor plant health

In this episode of the Concentrating on Chromatography podcast, host David Oliva interviews Ainslie Chen about her cutting-edge research on hemoglobin variant testing using liquid chromatography-high resolution mass spectrometry (LC-HR-MS). Ainslie discusses her novel method for separating and identifying hemoglobin subunits with mass shifts less than 1 Da, overcoming critical limitations in conventional clinical assays.Hear firsthand how her team developed an LC-HR-MS workflow using a C4 reversed-phase column, tackled technical challenges (including why C18 columns fell short), and achieved unprecedented sensitivity for variant detection in both adult and neonatal samples. The conversation touches on the potential of mass spectrometry to revolutionize clinical diagnostics, the importance of reproducibility, barriers to adoption in routine labs, and how teamwork drives scientific innovation.Perfect for analytical chemists, clinical laboratorians, and anyone interested in the future of protein variant analysis, this episode also features Ainslie’s advice for early-career scientists and insights from her first ACS poster presentation.<h4>Key Highlights:</h4><ul><li>Motivation behind improving hemoglobinopathy testing &nbsp;</li><li>Technical advantages of LC-HR-MS over gene sequencing and CE &nbsp;</li><li>The crucial role of the C4 column in resolving challenging samples &nbsp;</li><li>Critical parameters for reproducible LC-HR-MS workflows &nbsp;</li><li>Barriers and outlook for clinical adoption of advanced mass spectrometry &nbsp;</li><li>The value of teamwork and mentorship in scientific research &nbsp;</li><li>Direct insights from recent ACS conference experiences</li></ul><h3>Video transcription</h3>In this interview, Ainslie Chen discusses her research on improving the detection and identification of hemoglobin variants using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS). The work addresses limitations of conventional hemoglobinopathy testing, which often struggles to resolve very small mass differences between closely related hemoglobin subunits. Because even a single amino-acid substitution can significantly alter hemoglobin structure and function, high analytical resolution is essential for accurate diagnosis of conditions such as anemia and sickle cell disease.Chen explains why her team selected LC-HRMS over established clinical techniques like capillary electrophoresis and gene sequencing. While sequencing offers excellent accuracy, it is time-consuming, costly, and requires extensive sample preparation, making it impractical for routine clinical workflows. Capillary electrophoresis enables subunit separation but lacks the resolving power needed to distinguish variants differing by less than one Dalton. In contrast, high-resolution mass spectrometry provides both structural specificity and sensitivity, enabling confident identification of subtle hemoglobin variants with minimal sample preparation.A key methodological insight from the project was the use of a C4 reversed-phase LC column instead of the more common C18 column. Chen describes how the shorter carbon chain and larger pore size of C4 columns improved protein elution and reduced denaturation, leading to more reproducible separations. Critical parameters for reproducibility included consistent mobile-phase composition (0.1% formic acid in water and acetonitrile), controlled temperature (60 °C for LC, ~320 °C capillary temperature), and stable chromatographic conditions.Beyond hemoglobin analysis, Chen highlights the broader potential of high-resolution mass spectrometry in proteoform analysis and clinical diagnostics, including emerging applications in cancer detection. She acknowledges current barriers to clinical adoption—such as energy demands and instrument infrastructure—but points to advances like miniaturized mass spectrometers as promising steps toward wider use. The interview concludes with advice for young scientists: don’t hesitate to reach out to mentors, embrace collaboration, and view analytical chemistry as a versatile tool that bridges medicine, biology, environmental science, and beyond.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Unlocking Hemoglobin Variant Detection: LC-HR-MS Breakthrough with Ainslie Chen

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The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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Waters/Wyatt DAWN™ Multi Angle Light Scattering (MALS) Detector

The world's most advanced light scattering instrument for absolute characterization of proteins, conjugates, macromolecules, and nanoparticles.

<h5>Premier MALS</h5>The DAWN instrument is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution. It offers the highest sensitivity, the widest range of molecular weight, size, and concentration, and the largest selection of configurations and optional modules for enhanced capabilities.<h5>Separation First</h5>MALS is most often used in conjunction with a separation system such as Gel Permeation Chromatography (GPC-MALS), Size Exclusion Chromatography (SEC-MALS), or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size, and composition, independent of column calibration by reference standards.<h5>Supported by ASTRA ™ &nbsp;Software</h5>ASTRA Software and its built-in analysis modules support the DAWN MALS instrument, providing the most versatile analysis available for online multi-angle and dynamic light scattering measurements.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_DAWN_MALS_HPLC_detektor_konfigurace_d5b83e46b2.jpg" alt="Waters DAWN™ Multi Angle Light Scattering (MALS) Instrument: ASTRA Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_DAWN_MALS_HPLC_detektor_konfigurace_d5b83e46b2.jpg 245w," sizes="100vw" width="1500" height="807"><h4>Product description</h4>The DAWN and its companion Optilab ™ &nbsp;dRI detector are the established benchmarks for MALS analysis, cited in thousands of peer-reviewed publications. Multi-angle light scattering detection is indispensable for use with GPC and HPLC-SEC in order to obtain reliable molecular mass distributions and information on molecular conformation, branching ratio, fragments and aggregates.SEC-MALS with a DAWN and Optilab characterizes conformation and branching of synthetic and natural polymers larger than 10 nm in rms radius R g . The addition of a ViscoStar ™&nbsp; differential viscometer creates a GPC triple-detection system that can analyze molecular conformation and size below the R g &nbsp;limit of 10 nm and determine Mark-Houwink Sakurada coefficients.The combination of a UV/Vis absorbance detector with a DAWN instrument and Optilab refractive index detector creates a UV-MALS-RI triple-detection system, for analysis of copolymers and protein conjugates (glycoproteins, PEGylated proteins, surfactant-bound membrane proteins, etc.). With the addition of a WyattQELS ™ &nbsp;DLS module for size determination, protein conformation may be analyzed.<ul><li>Mw &nbsp;from 200 Da to 1000 MDa</li><li>Sensitivity: 10 ng/mL 100 kDa polystyrene in THF, 0.4 µg/mL BSA (66.4 kDa) in PBS</li><li>Rg &nbsp;from 10 to 500 nm, up to 1000 nm with shape-specific models</li><li>Ultra-stable, high-power laser</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_DAWN_MALS_HPLC_detektor_konfigurace_1_98811a1e87.jpg" alt="Waters DAWN™ Multi Angle Light Scattering (MALS) Instrument: Accessories" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_DAWN_MALS_HPLC_detektor_konfigurace_1_98811a1e87.jpg 213w," sizes="100vw" width="1000" height="733">The DAWN may also be used in batch (off-line) mode to characterize the weight-average molar mass, z-average size and second virial coefficient of unfractionated samples.In conjunction with a Calypso ™ &nbsp;CG-MALS system, the DAWN characterizes macromolecular interactions, label-free and immobilization-free: affinity and absolute stoichiometry of self- and hetero-association of simple or complex molecular systems. The Calypso system also automates the determination of second virial coefficients, cross virial coefficients, average molar masses and sizes.<h5>Ultrasonic Cleaning Included</h5>Each DAWN instrument includes an embedded COMET ™ &nbsp;ultrasonic transducer that cleans the flow cell automatically by agitating the fluid and loosening particles from the glass surfaces.<h5>Temperature-Controlled Models</h5><ul><li>Ambient model</li><li>Ultra-High-Temperature (UHT) model, from ambient up to +210 °C</li><li>Standard Peltier heated/cooled (H/C) model, temperature control from -15 °C to +150 °C</li></ul><h4>Specifications</h4><h5>Measurement Capabilities</h5><ul><li>Molar Mass Range: From 200 Da to 1 GDa, depending on dn/dc, sample concentration, and chromatography conditions.</li><li>Molecular Size (Radius of Gyration, Rg): Approximately 10 nm to 500 nm, extendable to 1000 nm with shape-specific models.</li><li>Hydrodynamic Radius (Rh):<ul><li>Flow mode: 0.5 nm to 300 nm (depending on configuration and flow rate).</li><li>Batch mode: 0.5 nm to 1 µm (requires WyattQELS™ DLS module or DynaPro™ NanoStar™ with fiber optic connection).</li></ul></li><li>Sensitivity: Detects as little as 10 ng of 100 kDa polystyrene in THF or 200 ng of BSA in PBS, using a standard 7.8 mm x 300 mm SEC column and no post-column filter.</li></ul><h5>Fluidics and Compatibility</h5><ul><li>Mobile Phase: Compatible with both aqueous and organic solvents.</li><li>Wetted Materials: 316 stainless steel, fused silica or F2 glass, and Kalrez.</li></ul><h5>Optical System and Detectors</h5><ul><li>MALS Detectors: 18 photodiodes with high gain and wide dynamic range for multi-angle light scattering.</li><li>Auxiliary Detectors:<ul><li>Laser monitor for stabilization</li><li>Forward transmission monitor to improve data quality and correct for sample absorption</li></ul></li><li>Dynamic Range: 3,300,000:1</li><li>Optional DLS Detection:<ul><li>Integrated WyattQELS™ module inside the DAWN</li><li>External fiber optic connection with DynaPro NanoStar or Mobius DLS-ELS instruments</li></ul></li></ul><h5>Laser Specifications</h5><ul><li>Wavelength: Standard 658 nm ± 4 nm; optional 785 nm for fluorescent samples</li><li>Power Control: Programmable from 10% to 100%</li></ul><h5>Flow Cell Options</h5><ul><li>Fused Silica: Ideal for solvents with refractive index &lt; 1.50</li><li>F2 Glass: Recommended for solvents with refractive index &gt; 1.50</li></ul><h5>Temperature Control Options</h5><ul><li>Ambient operation</li><li>Heated/Cooled (HC): -15 °C to +150 °C</li><li>Ultra-High Temperature (UHT): Ambient to +210 °C</li></ul><h5>Electronics and Connectivity</h5><ul><li>Analog Inputs: 4 differential inputs, 24-bit resolution, range -10 V to +10 V</li><li>Analog Outputs: 2 outputs from selectable measurement channels, -10 V to +10 V</li><li>I/O Options: Alarm in/out, auto-inject control, and Orbit™ valve integration</li><li>Computer Interface: Ethernet</li><li>Display: 10.1” high-resolution capacitive touchscreen with multi-touch controls</li></ul><h5>Instrument Dimensions</h5><ul><li>Size: 58 cm (L) x 36 cm (W) x 26 cm (H)</li></ul><h4>Other MALS Instruments</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1115">miniDAWN™</a> &nbsp;- Second only to the DAWN in sensitivity. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 10 MDa and radii from 10 – 50 nm. Ambient only.</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1116">microDAWN™</a>&nbsp; - The first MALS detector for UHPLC, with interdetector dispersion as low as 1.5 µL. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 20 MDa and radii from 10 – 50 nm. Ambient only.</li><li>ultraDAWN™ &nbsp;- Measures multi-angle light scattering and reports the results – weight-average molecular weight Mw &nbsp;and rms radius Rg &nbsp;– in real time.</li></ul>

Waters/Wyatt miniDAWN™ Multi Angle Light Scattering (MALS) Detector

The Best in Basic Multi-Angle Light Scattering for Absolute Macromolecular Analysis.

<h4>Overview</h4><h5>Molar Mass</h5>The miniDAWN determines absolute molar mass and size to characterize proteins, peptides, small polymers and nanoparticles in solution. Coupled to size exclusion chromatography (SEC or GPC), it eliminates the uncertainty and hassle of column calibration, for true confidence in macromolecular characterization.<h5>Superb Sensitivity</h5>The miniDAWN not only offers superb sensitivity—it further enhances productivity thanks to greatly enhanced field serviceability and easy data analysis with One-Click Mw™ .<h5>Compatible with ASTRA™&nbsp;</h5>The miniDAWN is accompanied by ASTRA, the most versatile software package available for analysis of online multi-angle and dynamic light scattering. ASTRA and the miniDAWN incorporate advanced ease-of-use features that help guarantee excellent measurements with a single click.<h4>Product Description</h4><h5>Molar mass, absolutely</h5>Wyatt's miniDAWN is most often used in conjunction with size exclusion chromatography (SEC-MALS) or field-flow fractionation (FFF-MALS) to determine distributions of molecular weight, size and composition. No need to make assumptions about molecular conformation or ideal column interactions.<h5>Characterize:</h5><ul><li>Proteins, small peptides and oligonucleotides</li><li>Synthetic and natural polymers</li><li>Nanoparticles, virus-like particles and vesicles</li></ul><h5>Measurement Range:</h5><ul><li>MW from 200 Da to 10 MDa</li><li>rg &nbsp;from 10 to 50 nm, up to 150 nm with shape-specific models</li><li>sensitivity down to 25 ng of 100 kDa polystyrene in THF, injected on a standard GPC column</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Mini_DAWN_urcuje_molekulovou_hmotnost_nezavisle_na_kalibraci_kolony_Zde_MALS_ukazuje_ze_nejdrive_eluujici_molekula_nema_nejvyssi_molarni_hmotnost_509e5530ea.jpg" alt="Waters: The miniDAWN determines molecular weight independently of column calibration. Here MALS shows that the earliest-eluting molecule does not have the highest molar mass." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Mini_DAWN_urcuje_molekulovou_hmotnost_nezavisle_na_kalibraci_kolony_Zde_MALS_ukazuje_ze_nejdrive_eluujici_molekula_nema_nejvyssi_molarni_hmotnost_509e5530ea.jpg 245w," sizes="100vw" width="820" height="438">The miniDAWN's performance is exceeded only by the DAWN™ which covers a wider range of size and molecular weight, with even better sensitivity and additional optional modules.<h5>Ultrasonic Cleaning Included</h5>Each miniDAWN includes an embedded COMET™ ultrasonic transducer that cleans the flow cell automatically by agitating the fluid and loosening particles from the glass surfaces.<h5>Modularity = Productivity</h5>The miniDAWN incorporates a fully modular design. Each module can be tested independently by the software to verify that it is functioning correctly. This imparts nearly complete field serviceability, since malfunctioning modules can be replaced and validated on-site by service engineers with no further calibration or alignment.The miniDAWN is also field-upgradeable: the embedded WyattQELS™ DLS Module for measuring hydrodynamic radii from 0.5 to 50 nm can be installed on-site as well as in new units shipped from the factory.<h5>Not just SEC-MALS...</h5>Wyatt's miniDAWN may be used in batch (off-line) mode or in conjunction with a Calypso™ CG-MALS system for characterization of average solution properties as well as macromolecular interaction properties including protein-protein binding affinity and complex stoichiometry.<h5>Simplicity personified</h5>With miniDAWN and ASTRA™, SEC-MALS became a whole lot easier. Sample-specific methods are set up in three short steps with ASTRA's Method Wizard, and new capabilities for automatic data processing mean that it takes just one click from your sample to a full report of the molecular weight and size results.The miniDAWN's System Ready monitor will even let you know when the system is fully equilibrated with low baseline noise, ready to run.<h4>Specifications</h4><h5>Measurement Capabilities</h5><ul><li>Molar Mass Range: 200 Da to 10 MDa for proteins; up to 1 MDa for linear polymers (depending on dn/dc, sample concentration, and chromatography conditions).</li><li>Molecular Size (Radius of Gyration, Rg): Approximately 10 nm to 50 nm, extendable up to 150 nm with shape-specific modeling.</li><li>Hydrodynamic Radius (Rh):<ul><li>Flow mode: 0.5 nm to 50 nm (optimal at flow rates ≤ 0.3 mL/min)</li><li>Batch mode: 0.5 nm to 1 µm (requires WyattQELS™ module or DynaPro™ NanoStar™ with fiber optic connection)</li></ul></li><li>Sensitivity: Detects as little as 25 ng of 100 kDa polystyrene in THF or 500 ng of BSA in PBS using a standard 7.8 mm x 300 mm SEC column with no post-column filter.</li></ul><h5>Fluidics and Compatibility</h5><ul><li>Mobile Phase Compatibility: Compatible with both aqueous and organic solvents.</li><li>Wetted Materials: 316 stainless steel, fused silica, and Kalrez.</li></ul><h5>Optical System and Detectors</h5><ul><li>MALS Detectors: Three high-gain, high dynamic range photodiodes at fixed angles.</li><li>Auxiliary Detectors:<ul><li>Laser monitor for stability control</li><li>Forward transmission monitor for absorbing samples and data quality evaluation</li></ul></li><li>Dynamic Range: 3,300,000:1</li><li>Optional DLS Detection:<ul><li>Integrated WyattQELS™ module within the miniDAWN chassis</li><li>External fiber optic connection from DynaPro™ NanoStar or Mobius™ DLS-ELS instruments</li></ul></li></ul><h5>Laser Specifications</h5><ul><li>Wavelength: 658 nm ± 4 nm</li><li>Power Control: Programmable from 10% to 100%</li></ul><h5>Temperature Control</h5><ul><li>Sample Temperature: Ambient</li></ul><h5>Electronics and Connectivity</h5><ul><li>Analog Inputs: 2 differential inputs, 24-bit resolution, ±10 V range</li><li>Analog Outputs: 1 output from a selectable measurement channel, ±10 V range</li><li>I/O Ports: Alarm in/out, auto-inject control, Orbit™ recycle valve support</li><li>Computer Interface: Ethernet</li><li>Data Rate: Up to 36.6 Hz</li></ul><h5>User Interface and Display</h5><ul><li>Front Panel: 7” (178 mm) high-resolution multi-touch display for real-time signal viewing, diagnostics, and settings</li></ul><h5>Instrument Dimensions</h5><ul><li>Size: 58 cm (L) x 36 cm (W) x 18 cm (H)</li></ul><h4>Other MALS Instruments</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1114">DAWN™</a> - The most sensitive MALS detector available, anywhere. Incorporates detectors at 18 angles to determine molar masses from 200 Da to 1 GDa and radii from 10 – 500 nm.<ul><li>Standard option: ambient temperature</li><li>Heated/cooled option: -15 °C to +150 °C</li><li>High-temperature option: ambient to +210 °C</li><li>The DAWN offers special options to handle fluorescent samples: fluorescence-blocking filters and an infrared, 785 nm laser.</li></ul></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1116">microDAWN™</a> - The first MALS detector for UHPLC, with interdetector dispersion as low as 1.5 µL. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 20 MDa and radii from 10 – 50 nm. Ambient only.</li><li>ultraDAWN™ - Measures multi-angle light scattering and reports the results – weight-average molecular weight Mw &nbsp;and rms radius Rg &nbsp;– in real time.</li></ul>

Waters/Wyatt microDAWN™ Multi Angle Light Scattering (MALS) Detector

The microDAWN can be coupled to any UHPLC-SEC system to determine absolute molecular weights and sizes of polymers, peptides, or proteins directly, without resorting to column calibration or reference standards.

<h4>Overview</h4><h5>UHPLC-SEC System</h5>The microDAWN can be coupled to any UHPLC-SEC system to determine absolute molecular weights and sizes of polymers, peptides, or proteins directly, without resorting to column calibration or reference standards. The microDAWN produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC, while meeting or exceeding the sensitivity of the miniDAWN™.<h4>Product Description</h4>The microDAWN MALS detector performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects the microDAWN is similar to the well-established miniDAWN™, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius.Distinctly from the miniDAWN, the microDAWN is uniquely suitable for use in conjunction with UHPLC Size Exclusion Chromatography (SEC-MALS) thanks to its small interdetector dispersion. In some cases it may be used with ion-exchange or other types of chromatography. Regardless of the specifics, the microDAWN produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_micro_DAWN_MALS_HPLC_detektor_konfigurace_1_4e888650cf.jpg" alt="Waters microDAWN™ Multi Angle Light Scattering (MALS) Instrument: Experimental setup" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_micro_DAWN_MALS_HPLC_detektor_konfigurace_1_4e888650cf.jpg 229w," sizes="100vw" width="894" height="609"><ul><li>Mw&nbsp; from 200 Da to 10 MDa</li><li>Sensitivity: 1.0 µg/mL BSA (66.4 kDa) in PBS; 50 ng/mL 100 kDa polystyrene in THF.</li><li>Rg &nbsp;from 10 to 50 nm</li><li>Ultra-stable laser</li></ul>The microDAWN and its companion microOptilab™ Refractive Index Detector interface to most standard UHPLC systems. As these detectors are based on the advanced technology of Wyatt's miniDAWN and Optilab™, respectively, users may be confident in the underlying capabilities cited in thousands of peer-reviewed publications.<h5>Ultrasonic Cleaning Included</h5>Each microDAWN includes a COMET™ ultrasonic transducer that cleans MALS flow cells automatically by agitating the fluid and loosening particles from the glass surfaces.<h4>Specifications</h4><h5>Measurement Capabilities</h5><ul><li>Molar Mass Range: From 200 Da to 10 MDa for proteins; up to 1 MDa for linear polymers (Typical, depending on dn/dc, sample concentration, and chromatography conditions)</li><li>Molecular or Particle Size (Radius of Gyration, Rg): 10 nm to 50 nm, extendable up to 150 nm with shape-specific modeling</li><li>Hydrodynamic Radius (Rh):<ul><li>Flow mode: 0.5 nm to 30 nm</li><li>Batch mode: 0.5 nm to 1 µm (Requires WyattQELS™ DLS module or DynaPro™ NanoStar™ with fiber optic connection)</li></ul></li><li>Sensitivity: Detects as little as 3.5 ng of 100 kDa polystyrene in THF or 70 ng of BSA in PBS, assuming use of a 15 cm UHPLC-SEC column with no post-column filter</li></ul><h5>Optical System and Detectors</h5><ul><li>MALS Detectors: Three high-gain photodiodes with broad dynamic range for multi-angle light scattering</li><li>Dynamic Range: 3,300,000:1</li><li>Auxiliary Detectors:<ul><li>Laser monitor for stabilization feedback</li><li>Forward transmission monitor for absorbing samples and data quality assessment</li></ul></li><li>Optional DLS Detection:<ul><li>Integrated WyattQELS™ module within the microDAWN chassis</li><li>External DLS connection via fiber optic from DynaPro™ NanoStar or Mobius™ DLS-ELS instruments</li></ul></li></ul><h5>Laser Specifications</h5><ul><li>Wavelength: 658 nm ± 4 nm</li><li>Power Control: Programmable from 10% to 100%</li></ul><h5>Sample Temperature and Flow Characteristics</h5><ul><li>Sample Temperature: Ambient</li><li>Interdetector Band Broadening:<ul><li>Upstream detector to microDAWN: &lt; 1.5 µL</li><li>Upstream to downstream detector: &lt; 1.6 µL</li></ul></li></ul><h5>Material Compatibility</h5><ul><li>Wetted Materials: 316 stainless steel, fused silica, PEEK, PTFE, and MP35N (iron-free alloy)</li></ul><h5>Electronics and Connectivity</h5><ul><li>Analog Inputs: 2 differential inputs, 24-bit resolution, ±10 V input range</li><li>Analog Outputs: 1 output from a user-selectable measurement channel, ±10 V range</li><li>I/O Ports: Alarm in/out, auto-inject control, Orbit™ valve integration</li><li>Computer Interface: Ethernet</li><li>Data Transmission Rate: Up to 73 Hz</li></ul><h5>User Interface</h5><ul><li>Display: 7” (178 mm) high-resolution multi-touch screen for signal monitoring, instrument settings, and diagnostics</li></ul><h5>Instrument Dimensions</h5><ul><li>Size: 58 cm (L) x 36 cm (W) x 18 cm (H)</li></ul><h4>Other MALS Instruments</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1114">DAWN™</a> - The most sensitive MALS detector available, anywhere. Incorporates detectors at 18 angles to determine molar masses from 200 Da to 1 GDa and radii from 10 – 500 nm.<ul><li>Standard option: ambient temperature</li><li>Heated/cooled option: -15 °C to +150 °C</li><li>High-temperature option: ambient to +210 °C</li><li>The DAWN offers special options to handle fluorescent samples: fluorescence-blocking filters and an infrared, 785 nm laser.</li></ul></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1115">miniDAWN™</a> - Second only to the DAWN in sensitivity. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 10 MDa and radii from 10 – 50 nm. Ambient only.</li><li>ultraDAWN™ - Measures multi-angle light scattering and reports the results – weight-average molecular weight Mw &nbsp;and rms radius Rg &nbsp;– in real time.</li></ul>

Products from category MALS detectors focused on GPC/SEC distributed by Waters Corporation (Europe) - page 1

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