<p><strong>Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal </strong><i><strong>Nature Chemical Biology</strong></i><strong>.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.</p><p>In their study,<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en"><strong>Dr. Hana Cahová</strong></a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.</p><p>The work was significantly shaped by <strong>Valentina Serianni from Hana Cahová’s team</strong>, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by <strong>Jana Škerlová</strong>, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.</p><p>Hana Cahová adds: <i>“We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by<strong> Dr. Tomáš Kouba</strong>, one of the authors of the study. <i>“Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,”</i> he explains.</p><p>Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en"><strong>cryo-EM center</strong></a><strong> </strong>that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"></p><blockquote><h4><strong>The Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5"><strong>Molecular insight into 5′ RNA capping with Np<sub>n</sub>Ns by bacterial RNA polymerase</strong></a></h4><p>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;</p><p>Nat. Chem. Biol. 21 (2026)</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from <i>Thermus</i> <i>thermophilus</i>. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</p></div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

<p><strong>Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"></p><h4><strong>The Role of Chromatography in Forensic Food Analysis</strong></h4><p>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:</p><ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate<br>adulteration.</li></ul><p>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.</p><h4><strong>Isocratic vs. Gradient Elution</strong></h4><p>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:</p><ul><li><strong>Isocratic HPLC </strong>uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li><strong>Gradient HPLC</strong> changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul><p>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.</p><h4><strong>Watrex Praha and the Contribution of Modern Systems to Food Analysis</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy"><strong>Watrex Praha, s.r.o.</strong></a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.</p><p>Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline"><strong>Streamline™ product line</strong></a><strong> </strong>includes several HPLC system concepts suitable for food analysis:</p><ul><li><strong>Isocratic HPLC system</strong> – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li><strong>Binary gradient HPLC system</strong> – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures<br>(e.g., phenolic profiles of wine, components of aromatic extracts).</li><li><strong>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography)</strong> – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf"><strong>Product catalogue HERE</strong></a></p></blockquote><p>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.</p><h4><strong>Practical Examples of Adulteration Revealed by HPLC</strong></h4><ul><li><strong>Detection of olive oil dilution: </strong>HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li><strong>Estimation of sugar addition in honey:</strong> Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li><strong>Identification of aromatic adulterants in wine:</strong> Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4><strong>Conclusion</strong></h4><p>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy"><strong>Watrex Praha</strong></a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.</p><blockquote><p>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy"><strong>Watrex Praha</strong></a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true"><strong>LinkedIn</strong></a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague"><strong>Facebook</strong></a>) for more engaging topics.</p></blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

<p><strong>Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.</strong></p><h4><strong>In this episode, you'll learn:</strong></h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul><p>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.</p><h4><strong>Referenced Papers:</strong></h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul><p>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.</p><h3><strong>Video Transcription</strong></h3><h4><strong>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</strong></h4><h5><strong>Introduction</strong></h5><p>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.</p><h5><strong>Research Focus: Malaria Drug Discovery and Kinase Targets</strong></h5><p>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.</p><p>This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.</p><p>Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.</p><h5><strong>Chromatography in Protein Expression and Purification Workflows</strong></h5><p>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.</p><p>Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.</p><p>A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.</p><h5><strong>Integrating Size Exclusion Chromatography into the Workflow</strong></h5><p>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.</p><p>To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.</p><p>These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.</p><h5><strong>The Role of Mass Spectrometry in Biomolecular Characterization</strong></h5><p>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.</p><p>Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.</p><p>These approaches are essential for understanding how candidate drug targets function within complex biological systems.</p><h5><strong>Sample Preparation Challenges in Biological Research</strong></h5><p>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.</p><p>Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.</p><h5><strong>Advances in Chromatography and MS for Malaria Research</strong></h5><p>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.</p><p>In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.</p><p>Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.</p><h5><strong>Malaria, Drug Resistance, and Global Impact</strong></h5><p>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.</p><p>During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.</p><p>Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.</p><h5><strong>Advice for Early-Career Researchers</strong></h5><p>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.</p><p>Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.</p><p>Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.</p><h5><strong>Closing Remarks</strong></h5><p>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

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Food

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Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

Phenomenex Kinetex Core-Shell HILIC Preparative HPLC Columns

Kinetex HILIC is a core-shell particle with unbonded silica, providing selectivity for retaining and separating polar compounds in HILIC chromatography.

<p><strong>Hydrophilic Polar Selectivity and Performance!</strong></p><p>The Kinetex HILIC is a core shell based particle with a unbonded silica phase is used under hydrophilic interaction chromatography conditions to provide the selectivity needed for the retention and <strong>separation of polar compounds</strong>. This versatile column can easily upgrade existing fully porous 5 µm and 3 µm methods to <strong>shorten run time</strong>, <strong>increase sensitivity</strong>, and even provide <strong>greater resolution</strong> with much <strong>higher efficiency levels</strong>.</p><ul><li>Compatible with All Analytical HPLC/UHPLC Systems</li><li>Unique Polar Retentive L3 Silica</li><li>Unbonded Silica Phase</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_Core_Shell_HILIC_HPLC_Column_9ff82b0e20.webp" alt="Phenomenex: Kinetex Core-Shell HILIC HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_Core_Shell_HILIC_HPLC_Column_9ff82b0e20.webp 245w," sizes="100vw" width="500" height="150"></p><ul><li><strong>Carbon Load</strong>: 0 %</li><li><strong>Particle Size (μm)</strong>: 5</li><li><strong>Recommended Use</strong>: Retention and separation of polar compounds hydrophilic interaction chromatography conditions</li></ul><h4><strong>Alternative Polar Selectivity to a C18 &nbsp;</strong></h4><p>A Core-Shell based silica phase that combines high-performance with novel polar HILIC selectivity.</p><h4><strong>Increase Polar Retention</strong></h4><p>Used under HILIC running conditions, this phase provides the highest polar selectivity for retention and separation of hydrophilic compounds.</p><blockquote><p class="cke-user_style-important"><strong>Stationary phase Kinetex Core-Shell HILIC is provided in these column types: analytical and preparative</strong></p></blockquote><p><strong>Axia Packed Preparative Columns</strong></p><ul><li><strong>Column length (mm): </strong>100, 150, 250</li><li><strong>Column ID (mm):</strong> 21.2</li><li><strong>Particle size (µm):</strong> 5</li></ul><h3><strong>Kinetex Core-Shell Technology Columns</strong></h3><p>Kinetex Core-Shell Technology delivers dramatic<strong> improvements in efficiency</strong> over conventional fully porous media, which can be leveraged to <strong>increase resolution</strong>, significantly <strong>improve productivity</strong>, r<strong>educe solvent consumption</strong>, and <strong>decrease costs</strong>. Whether you are running high-performance liquid chromatography (<strong>HPLC)</strong> or ultra-high performance liquid chromatography (<strong>UHPLC</strong>) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. <strong>Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.</strong></p><p><strong>Advantages of Kinetex HPLC Columns</strong></p><ul><li>Performance gains on ANY liquid chromatography system</li><li>System-to-system and lab-to-lab method portability</li><li>Improve the productivity of older, established methods<strong>.</strong></li></ul><h4><strong>How Does Core-Shell Technology Work?</strong></h4><p><strong>Core-shell technology</strong> in chromatography is a revolutionary approach that <strong>enhances the efficiency of HPLC systems while minimizing costs.</strong> Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for <strong>faster analyte mass transfer</strong> <strong>between</strong> the <strong>mobile</strong> and <strong>stationary phases</strong>, as diffusion occurs only through the thin, porous shell rather than the entire particle. This <strong>minimizes band broadening</strong>, leading to <strong>higher separation efficiency</strong> and <strong>sharper peaks</strong>.</p><p>The <strong>consistency in size and shape</strong> of core-shell particles further <strong>enhances separation efficiency</strong> by reducing variability in analyte movement. As a result, core-shell HPLC columns provide <strong>high efficiency</strong> and <strong>resolution</strong> at <strong>lower</strong> <strong>backpressures</strong>, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.</p><p>Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.</p><h3><strong>Kinetex Core-Shell Performance on Any LC System</strong></h3><p>The 5 versatile particle sizes allow you to select the best fit for your instrument.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"></p><h3><strong>Particle Chemistry</strong></h3><p>Using sol-gel processing techniques that incorporate nano-structuring technology, a <strong>durable, homogeneous porous shell</strong> is grown on a <strong>solid silica core</strong> to create a <strong>Kinetex Core-Shell particle</strong>. This particle morphology results in <strong>less band broadening</strong> for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.</p><ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul><p><strong>Kinetex particles</strong> are characterized by an exceptionally <strong>consistent size distribution</strong>, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to <strong>enhanced separation efficiency</strong> and <strong>sharper peaks</strong>.</p><p>The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"></p><h3><strong>Core-shell vs Fully Porous</strong></h3><h4><strong>High-Efficiency‚ High-Density Particle</strong></h4><p>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.</p><p>The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"></p><h3><strong>Expand Your Core-Shell Selectivity Toolbox</strong></h3><p>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of <strong>11 diverse stationary phases</strong>, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.</p><p>Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.</p><h3><strong>Top 5 Pros of Kinetex Core-Shell Technology</strong></h3><ul><li><strong>Productivity</strong>: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li><strong>Portability </strong>The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li><strong>Performance</strong> Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li><strong>Phases </strong>With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li><strong>Pressure</strong> Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Watrex Deltasil 100 C18 Preparative HPLC columns

Need a high quality column at an affordable price? Deltasil™ columns are filled with standard reversed phase for a wide range of applications.

<p><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=watrex+deltasil+100+C18&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>Watrex DeltaSil™ 100 C18</strong></a><strong> column is a standard reversed phase for wide field of applications in analysis</strong><br><strong>of drugs, organic compounds, peptides and others.</strong></p><p>Stationary phase is based on <strong>synthetic 5 μm silica-gel with 100 Å pores and 15% carbon content</strong>. The column has a good peak symmetry and efficiency.</p><p>Theoretical plate count value for non-polar compounds, such as naphthalene, reaches <strong>up to 90.000 theoretical plates per meter</strong>.</p><p>A <strong>wide range of column sizes</strong> is available, with additional dimensions available on request.</p><p>We are committed to the <strong>sustainability</strong> of our columns and therefore also offer <strong>backfill</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Watrex_Deltasil_HPLC_column_Mobile_phase_85_ACN_Flowrate_0_65_mlmin_Pressure_54_bar_Detection_254_nm_Injection_2_ml_de7068538f.jpg" alt="Watrex: Watrex Deltasil HPLC column (Mobile phase 85% ACN; Flowrate 0.65 mlmin; Pressure 54 bar; Detection 254 nm; Injection 2 μl)"></p><h3><strong>Ordering information:</strong></h3><h5><strong>PN/Component:</strong></h5><ul><li><strong>WI02241</strong> Watrex Deltasil™ 100 C18, 10 μm, 250x20 mm column</li><li><strong>WI02242</strong> Watrex Deltasil™ 100 C18, 10 μm, 300x20 mm column</li><li><strong>WI02243</strong> Watrex Deltasil™ 100 C18, 5 μm, 250x20 mm column &nbsp;(<strong>Price 1 294 € ex VAT</strong>)</li><li><strong>WI02244</strong> Watrex Deltasil™ 100 C18, 5 μm, 300x20 mm column</li><li><strong>WI02245</strong> Watrex Deltasil™ 100 C18, 5 μm, 250x30 mm column</li><li><strong>WI02246</strong> Watrex Deltasil™ 100 C18, 10 μm, 250x30 mm column</li><li><strong>WI02247</strong> Watrex Deltasil™ 300 C18, 5 μm, 250x20 mm column</li><li><strong>WI02248</strong> Watrex Deltasil™ 300 C18, 10 μm, 250x20 mm column</li><li><strong>WI02249</strong> Watrex Deltasil™ 300 C18, 10 μm, 250x30 mm column</li><li><strong>WI02153</strong> Guard column insert 10x4.0 mm, Deltasil C18, 5 um, 10 pcs</li></ul>

Galochrom Chiral ION-QD HPLC Preparative Columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan.

<p><strong>Chiral ion exchanger GEN-QD is highly efficient in the enantiomer resolution of acidic compounds.</strong></p><p>This product is now available in high quality and at a reasonable price at our company!</p><p>To create our <strong>chiral sorbent</strong>, we use <strong>3µm fully porous spherical silica particles</strong>, which provide greater efficiency than the original product.</p><p>Additionally, our <strong>Galo-ION-QD-200</strong> is recommended for <strong>biological applications</strong> due to its larger pore size, which improves the separation efficiency for large molecules.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QD_HPLC_kolony_ION_QD_selector_10dd9c7ee5.jpg" alt="Galochrom: Galochrom Chiral ION-QD selector."></p><h5><strong>Premium Quality</strong></h5><p>In every manufactured product, we guarantee the highest standards of quality.</p><h5><strong>Cost-effective</strong></h5><p>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.</p><h5><strong>High-performance</strong></h5><p>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.</p><h3><strong>Ordering information</strong></h3><blockquote><p><strong>Stationary phase Chiral ION-QD is provided in these column types: analytical and preparative</strong></p></blockquote><h4><strong>Preparative Columns</strong></h4><ul><li><strong>Dimensions [mm]: 150 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION05-P150</li><li><strong>Price [€]: 9 800</strong></li></ul></li><li><strong>Dimensions [mm]: 250 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION06-P250</li><li><strong>Price [€]: 11 300</strong></li></ul></li></ul><blockquote><h4><strong>Get up to a 20% discount on your first purchase with us.</strong></h4></blockquote><h3><strong>Reach new heights</strong></h3><p>Spend more time on testing and less time on troubleshooting</p><blockquote><h4><strong>How can our unique columns help you succeed?</strong></h4><p>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:</p><ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4><strong>What makes Chiral </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1241"><strong>ION-QN</strong></a><strong> and ION-QD unique?</strong></h4><p><span style="font-size:20px;"><strong>Designed for stability</strong></span></p><p>The manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.</p><p><strong>The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376"></p><p>During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"></p><h3><strong>Achieve your results</strong></h3><p>Increase your productivity with uniform, stable, and reproducible results</p><h4><strong>High-Quality Silica</strong></h4><p>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.</p><p>In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a<br>result, our columns:</p><ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"></p><h4><strong>Elution Order</strong></h4><p>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers.<strong> Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545"></p><p>The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:</p><ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

Galochrom Chiral ION-QN HPLC Preparative Columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan. 

<p><strong>Our company is proud to offer the Galo ION-QN chiral ion exchanger, which is capable of efficiently separating enantiomers of acidic compounds.</strong></p><p>This product is now available in high quality and at a reasonable price at our company!</p><p>To create our <strong>chiral sorbent</strong>, we use <strong>3µm fully porous spherical silica particles</strong>, which provide greater efficiency than the original product.</p><p>Additionally, our <strong>Galo-ION-QN-200</strong> is recommended for <strong>biological applications</strong> due to its larger pore size, which improves the separation efficiency for large molecules.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QN_HPLC_kolony_ION_QN_selector_f3789cb2c1.jpg" alt="Galochrom: Galochrom Chiral ION-QN selector."></p><h5><strong>Premium Quality</strong></h5><p>In every manufactured product, we guarantee the highest standards of quality.</p><h5><strong>Cost-effective</strong></h5><p>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.</p><h5><strong>High-performance</strong></h5><p>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.</p><h3><strong>Ordering information</strong></h3><blockquote><p><strong>Stationary phase Chiral ION-QN is provided in these column types: analytical and preparative</strong></p></blockquote><h4><strong>Preparative Columns</strong></h4><ul><li><strong>Dimensions [mm]: 150 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION03-P150</li><li><strong>Price [€]: 9 800</strong></li></ul></li><li><strong>Dimensions [mm]: 250 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION04-P250</li><li><strong>Price [€]: 11 300</strong></li></ul></li></ul><blockquote><h4><strong>Get up to a 20% discount on your first purchase with us.</strong></h4></blockquote><h3><strong>Reach new heights</strong></h3><p>Spend more time on testing and less time on troubleshooting</p><blockquote><h4><strong>How can our unique columns help you succeed?</strong></h4><p>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:</p><ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4><strong>What makes Chiral ION-QN and </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1240"><strong>ION-QD</strong></a><strong> unique?</strong></h4><p><span style="font-size:20px;"><strong>Designed for stability</strong></span></p><p>The manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.</p><p><strong>The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376"></p><p>During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"></p><h3><strong>Achieve your results</strong></h3><p>Increase your productivity with uniform, stable, and reproducible results</p><h4><strong>High-Quality Silica</strong></h4><p>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.</p><p>In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a<br>result, our columns:</p><ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"></p><h4><strong>Elution Order</strong></h4><p>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers.<strong> Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545"></p><p>The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:</p><ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

Thermo Scientific Syncronis C18 HPLC Columns

Attain repeatable and consistent LC separations for your scale up purification needs with Thermo Scientific™ Syncronis™ C18 reversed phase preparative HPLC columns. 

<p><strong>Attain repeatable and consistent LC separations for your scale up purification needs with Thermo Scientific™ Syncronis™ C18 reversed phase preparative HPLC columns. These preparative HPLC columns have extremely pure, high surface area silica with dense stationary phase coverage to minimize secondary interactions and ensure robust, high-resolution separations. Double endcapping provides additional surface coverage and inertness toward basic compounds. Columns come in a variety of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed.</strong></p><h5><strong>Highly Reproducible Separations</strong></h5><ul><li>Rigorously tested stationary phase bonded to the highest quality silica ensures consistent, predictable LC performance.</li></ul><h5><strong>Enhanced Resolution and Sensitivity</strong></h5><ul><li>Dense surface coverage of silica minimizes secondary interactions and peak tailing.</li><li>Double endcapped for extra surface coverage.<br>C18 (USP L1) selectivity.</li></ul><p><strong>Preparative HPLC Column Hardware Options</strong> - based on column inner diameter (i.d.) and column performance needs.</p><h5><strong>Standard Threaded and Standard Flange Hardware</strong></h5><ul><li>Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.</li><li>Standard threaded hardware for column i.d. &lt; 40mm.</li><li>Standard flange hardware for column i.d. ≥ 40mm.</li><li>Standard hardware can be modified on request to be compatible with SFC.</li></ul><h5><strong>XtendedLife Hardware</strong></h5><ul><li>Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.</li><li>Compatible with SFC and is available in dimensions up to 50mm i.d.</li></ul><p>Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.</p><h5><strong>Customer Specific Orders</strong></h5><p>If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in a preparative scale not shown or would like to discuss a specific column dimension, we would be happy to discuss your requirements.<br>Please contact your local sales representative for Customer Specific Orders.<br>Custom built columns are not eligible for returns.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Column type:</strong> Reversed Phase</li><li><strong>Endcapped:</strong> yes</li><li><strong>Max. Pressure:</strong> 4300 psi (295 bar)</li><li><strong>Packing Material:</strong> Spherical, Fully Porous, Ultrapure Silica</li><li><strong>Stationary phase:</strong> C18</li><li><strong>USP Type:</strong> L1</li><li><strong>Surface Area:</strong> 320 m<sup>2</sup>/g</li><li><strong>Temperature:</strong> 60°C</li><li><strong>Carbon load: </strong>16%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>pH: </strong>2 to 9</li></ul><h4><strong>Syncronis HPLC and UHPLC Columns</strong></h4><h5><strong>Enter a new world of chromatography</strong></h5><p>When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. This makes selecting an HPLC column that offers highly-reproducible results essential to attaining this goal.</p><h5><strong>Features of Syncronis columns</strong></h5><p>Thermo Scientific Syncronis LC columns achieve high levels of consistency and reproducibility through:</p><ul><li>Highly-pure, high-surface-area silica</li><li>Dense bonding and double endcapping</li><li>Stringent manufacturing and testing protocols</li></ul>

Thermo Scientific Syncronis PREP HILIC HPLC Columns

Maintain consistent, predictable separations for your scale up purification needs with Thermo Scientific™ Syncronis™ HILIC preparative HPLC columns for polar and hydrophilic analytes.

<p><strong>Maintain consistent, predictable separations for your scale up purification needs with Thermo Scientific™ Syncronis™ HILIC preparative HPLC columns for polar and hydrophilic analytes. Highly pure, high surface area silica and dense stationary phase coverage minimize secondary interactions and deliver reproducible high-resolution separations.&nbsp;</strong></p><p>The zwitterionic stationary phase results in a neutral, highly polar surface and provides optional selectivity to C18 columns without ion-pairing or derivatization. Our columns come in a wide range of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed.</p><h5><strong>Alternative Selectivity</strong></h5><ul><li>Zwitterionic bonded phase enhances retention of polar and hydrophilic analytes.</li><li>No need for ion-pair or derivatization.</li><li>Enhanced sensitivity with MS detection.</li><li>Highly organic mobile phase facilitates desolvation in ESI-MS</li></ul><p><strong>Preparative HPLC Column Hardware Options</strong> - based on column inner diameter (i.d.) and column performance needs.</p><h5><strong>Standard Threaded and Standard Flange Hardware</strong></h5><ul><li>Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.</li><li>Standard threaded hardware for column i.d. &lt; 40mm.</li><li>Standard flange hardware for column i.d. ≥ 40mm.</li><li>Standard hardware can be modified on request to be compatible with SFC.</li></ul><h5><strong>XtendedLife Hardware</strong></h5><ul><li>Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.</li><li>Compatible with SFC and is available in dimensions up to 50mm i.d.</li></ul><p>Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Column type:</strong> HILIC</li><li><strong>Endcapped:</strong> No</li><li><strong>Max. Pressure:</strong> 2000 psi (140 bar)</li><li><strong>Packing Material:</strong> Spherical, Fully Porous, Ultrapure Silica</li><li><strong>Stationary phase:</strong> Zwitterionic</li><li><strong>Surface Area:</strong> 320 m<sup>2</sup>/g</li><li><strong>Temperature:</strong> 60°C</li><li><strong>Carbon load: </strong>5%</li><li><strong>Phase:</strong> HILIC</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>pH: </strong>2 to 8</li></ul><h4><strong>Syncronis HPLC and UHPLC Columns</strong></h4><h5><strong>Enter a new world of chromatography</strong></h5><p>When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. This makes selecting an HPLC column that offers highly-reproducible results essential to attaining this goal.</p><h5><strong>Features of Syncronis columns</strong></h5><p>Thermo Scientific Syncronis LC columns achieve high levels of consistency and reproducibility through:</p><ul><li>Highly-pure, high-surface-area silica</li><li>Dense bonding and double endcapping</li><li>Stringent manufacturing and testing protocols</li></ul>

Phenomenex Gemini C18 HPLC Columns

Gemini C18 is a robust reversed-phase HPLC stationary phase offering unique selectivity, high structural integrity, and increased loadability, making it ideal for preparative and purification applications.

<p><strong>Gemini C18 HPLC phase offers unique reversed phase selectivity, high structural integrity, and increased loadability which make it ideal for preparative and purification applications in pre-packed columns and bulk media.</strong></p><ul><li><strong>Carbon Load:</strong> 14, 14 %</li><li><strong>Surface Area (m²/g):</strong> 375</li><li><strong>Particle Size: </strong>3, 5, 10</li><li><strong>Recommended Use:</strong> Separation of basic compounds under high pH conditions</li></ul><h3><strong>Gemini HPLC Columns</strong></h3><h4><strong>Twin-NX technology HPLC columns. Extend your column lifetime</strong></h4><p>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405"></p><p>Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.</p>

Phenomenex Gemini NX-C18 HPLC Columns

Gemini NX-C18 is a rugged, high-efficiency reversed-phase HPLC column delivering controlled selectivity for ionizable compounds and reliable performance across high and low pH LC/MS workflows.

<p><strong>The most rugged and highly efficient Gemini C18 HPLC column phase for reversed phase. Gemini NX-C18 takes LC/MS separations to the next level and offers:</strong></p><ul><li>Control selectivity of ionizable compounds for optimized methods</li><li>Consistent performance in both volatile and non-volatile buffers</li><li>High and low pH methods</li></ul><h5><strong>Key features:</strong></h5><ul><li><strong>Carbon Load:</strong>14 %</li><li><strong>Surface Area (m²/g):</strong> 375</li><li><strong>Particle Size: </strong>3, 5, 10</li><li><strong>Recommended Use:</strong> High efficiency analytical or preparative separations under extreme pH conditions</li></ul><h3><strong>Gemini HPLC Columns</strong></h3><h4><strong>Twin-NX technology HPLC columns. Extend your column lifetime</strong></h4><p>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405"></p><p>Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.</p>

Phenomenex Gemini C8(3) HPLC Columns

Gemini C8(3) purification media is optimized for human insulin and its analogues, offering exceptional mechanical strength, high packing efficiency, and enhanced resistance to caustic regeneration conditions.

<h4><strong>Optimized Purification Media for Human Insulin and It's Analogues</strong></h4><ul><li>Enhanced Robustness Against Caustic Regeneration Conditions</li><li>High Packing Efficiencies</li><li>Incredible Mechanical Strength</li></ul><p>Typically insulin purification methodologies include vital steps for column efficiency tests, equilibration, loading, and multiple caustic washes. In particular, the high pH caustic washes are very important for the removal aggregate build up, however these wash steps typical diminish column lifetime quite substantially. With the improved chemical robustness and performance of Gemini C8(3) customers can now switch their focus to higher yields and purities and minimize operational down-time due to less frequent need to replace contaminated media.</p><h4><strong>QC Tested to ensure consistent selectivity for Insulin and Impurity</strong></h4><p>In conjunction with a media designed specifically for insulin purification, the Quality team at Phenomenex strives to ensure high reproducibility of Gemini 10µm C8(3) through numerous quality control steps throughout the manufacturing process, including a fit for purpose human insulin QC test. This elevated level of focus on quality ultimately results in a model product for large scale insulin purification.</p><h5><strong>Key features:</strong></h5><ul><li><strong>Carbon Load: </strong>-</li><li><strong>Surface Area (m²/g):</strong> -</li><li><strong>Particle Size: </strong>10</li><li><strong>Recommended Use:</strong> Separation of very hydrophobic compounds</li></ul><h3><strong>Gemini HPLC Columns</strong></h3><h4><strong>Twin-NX technology HPLC columns. Extend your column lifetime</strong></h4><p>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405"></p><p>Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.</p>

Phenomenex Gemini C6-Phenyl HPLC Columns

Gemini C6-Phenyl HPLC phase provides aromatic selectivity with low bleed performance, making it ideal for LC/MS analysis of aromatic, polar, and basic compounds under high pH conditions.

<p><strong>Gemini C6-Phenyl HPLC phase has a phenyl group bound to the organo-silica surface using a 6-carbon chain for a low bleed aromatic selective phase. Gemini C6-Phenyl is well suited for LC/MS applications.</strong></p><h5><strong>Key features:</strong></h5><ul><li><strong>Carbon Load: </strong>12 %</li><li><strong>Surface Area (m²/g):</strong> 375</li><li><strong>Particle Size: </strong>3, 5</li><li><strong>Recommended Use:</strong> Separation of aromatic, polar, or basic compounds under high pH conditions</li></ul><h3><strong>Gemini HPLC Columns</strong></h3><h4><strong>Twin-NX technology HPLC columns. Extend your column lifetime</strong></h4><p>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405"></p><p>Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.</p>

Products from category Preparative HPLC columns focused on LC columns - page 1

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