<div><p><strong>Two-dimensional liquid chromatography (2D-LC) was introduced to overcome the limited peak capacity of one-dimensional LC (1D-LC), which often fails with highly complex mixtures. By coupling two separation dimensions with distinct retention mechanisms, 2D-LC enhances resolution, selectivity, and analytical depth.</strong></p><p>Its effectiveness stems from orthogonality—the deliberate use of complementary mechanisms, such as reversed-phase and hydrophilic interaction chromatography (<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-hilic-chromatography"><strong>HILIC</strong></a>)—which maximizes peak capacity and enables the separation of compounds unresolved in 1D-LC.</p><p>2D-LC has become integral to diverse applications. In metabolomics and proteomics, it supports large-scale peptide, protein, and metabolite profiling. Pharmaceutical laboratories employ it for impurity profiling, stability studies, and regulatory testing, while environmental and food sciences use it to detect trace pollutants, pesticides, and other contaminants in complex matrices.</p><p>Recent advances, including integration with high-resolution mass spectrometry, automation, and AI-based data processing, have streamlined workflows and reduced labor intensity. Sustainability efforts, such as solvent recycling and miniaturization, further support its adoption. By combining precision, versatility, and scalability, 2D-LC represents a transformative platform for contemporary analytical science.</p></div><div><div><div><div><h3><strong>Principle and Working Mechanism of 2D-LC</strong></h3><p>Two-dimensional liquid chromatography (2D-LC) is based on the principle of orthogonality. This term refers to the degree of independence between two chromatographic separation mechanisms.</p><p>By selecting stationary and<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/hplc-knowledge-center/mobile-phase-in-chromatography"><strong>mobile phases</strong></a> that exploit different chemical properties such as polarity, hydrophobicity, ionic charge, or molecular size, 2D chromatography achieves superior resolving power. This orthogonality ensures that analytes co-eluting in the first dimension can be effectively separated in the second.</p><p>Peak capacity is one of the key performance metrics in 2D liquid chromatography. It approximates the product of the separation capacity of each dimension. For instance, a first dimension with a capacity of 100 and a second of 150 produces an estimated combined peak capacity of 15,000. This exponential gain enables comprehensive profiling of complex mixtures and enhances analytical confidence.</p><p>The workflow of 2D chromatography is systematic and highly automated. It begins with sample injection, after that, analytes are separated in the first column. Modulation or fractionation then transfers portions of this effluent to the second column, either in real-time or via fraction collection.</p><p>The second-dimension separation employs steep gradients and high-pressure systems to achieve rapid resolution. Finally, detection is carried out using UV-Vis, fluorescence, or mass spectrometry, providing both qualitative and quantitative insights.</p><p>Modern 2D-LC platforms incorporate advanced software and automation, which helps them to enable reproducibility, efficient data processing, and minimal operator intervention.</p></div></div></div></div><div><div><div><div><h3><strong>Modes of 2D-LC Operation</strong></h3></div></div></div></div><div><p>Two-dimensional liquid chromatography (2D-LC) can be performed in different modes. Each of these modes is designed to balance resolution, analysis time, and sample coverage. Two majorly used approaches are comprehensive 2D-LC (LC×LC) and heart-cutting 2D-LC (LC–LC). Comprehensive mode provides full sample coverage and the highest resolution; in contrast, heart-cutting mode offers a targeted and efficient approach for specific analytes.</p></div><div><div><div><div><h4><strong>Comprehensive 2D-LC (LC×LC)</strong></h4></div></div></div></div><div><p>In comprehensive 2D-LC, every fraction eluting from the first dimension undergoes further separation in the second dimension. This all-encompassing approach is ideal for untargeted studies, including metabolomics, proteomics, environmental monitoring, and materials science, where sample complexity is extreme.</p><p>LC×LC demands rapid second-dimension separations, generally executed on short sub-2 µm <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=48&amp;manufacturerItems=50">lc-columns</a> under ultrahigh-pressure liquid chromatography (<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/liquid-chromatography/formats-techniques/lc-formats-uhplc-columns"><strong>UHPLC</strong></a>) conditions. Its strength lies in delivering maximum peak capacity and ensuring no compound is mis-analyzed. However, this method generates large datasets, making advanced data handling, chemometric software, and visualization tools essential for interpretation.</p></div><div><div><div><div><h4><strong>Heart-cutting 2D-LC (LC–LC)</strong></h4></div></div></div></div><div><p>Heart-cutting 2D-LC is a selective mode in which only specific fractions from the first dimension are reanalyzed in the second. This targeted strategy reduces analysis time, conserves solvent, and focuses resolution where it is absolutely required. It is widely applied for impurity profiling, regulatory compliance testing, and focused metabolomics.</p><p>Innovations like multiple heart-cutting (mLC–LC) and selective comprehensive LC×LC (sLC×LC) combine precision with broader analytical coverage. It offers flexible workflows that can adapt to both discovery and routine quality control needs.</p></div><div><div><div><div><h3><strong>When and Why Use Two-Dimensional Liquid Chromatography</strong></h3><p>Two-dimensional liquid chromatography (2D-LC) is mainly chosen when traditional one-dimensional <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/hplc-knowledge-center/lc-an-indept-exploration"><strong>liquid chromatography</strong></a> (1D-LC) becomes inadequate to separate complicated mixtures. In most biological, pharmaceutical, and environmental samples, analytes tend to co-elute with similar physicochemical properties, which leads to difficult quantitation and identification.</p><p>The orthogonality of 2D-LC triumphantly overcomes these difficulties through high augmented peak capacity and resolution, providing an information depth that 1D-LC cannot possibly achieve. This strategy is especially useful in the study of intricate sample matrices. Plant extracts, biological fluids, foods, and environmental samples usually contain a vast number of compounds with comparable retention properties numbering in the thousands.</p><p>2D-LC enables the dissection of such chemical complexity to unmask minor constituents that might be biologically active or of environmental interest. It is now critical for untargeted metabolomics, lipidomics, environmental analysis, and natural product profiling, in which extensive coverage is preferred.</p><p>In regulated sectors like pharmaceuticals, two-dimensional chromatography is becoming commonly used to address stringent quality assurance and regulatory considerations. Where 1D-LC is unable to separate key impurities or degradation products, 2D-LC provides a robust platform for impurity profiling, stability testing, and lot-release assays. It reduces the effect of excipients and non-volatile buffer when using MS for impurity identification. The capacity to selectively target and define trace impurities delivers regulatory assurance and product safety.</p><p>Aside from resolution, 2D-LC provides greater sensitivity and quantitation. Simplifying matrix complexity reduces ion suppression in mass spectrometry processes, which enhances signal clarity and detection limits. This feature is essential for exploratory analysis, like finding new biomarkers or monitoring trace contaminants, where accuracy and reproducibility are of utmost importance.</p><p>With increasing analytical needs, 2D-LC is an ideal high-throughput, scalable, and future-proof solution for laboratories handling chemically complex and troublesome samples.</p></div></div></div></div><div><div><div><div><h3><strong>Common Applications of 2D-LC</strong></h3></div></div></div></div><div><p>Two-dimensional liquid chromatography (2D-LC) has evolved into a versatile analytical tool capable of resolving chemically complex samples that challenge conventional methods. By offering dramatically increased peak capacity and orthogonal selectivity, 2D-LC supports diverse industries ranging from pharmaceuticals to materials science. Below are some of its most impactful applications:</p></div><div><div><div><div><h4><strong>Biopharmaceuticals: mAbs, ADCs, and peptides</strong></h4></div></div></div></div><div><p>Biopharmaceutical development relies on 2D-LC to characterize monoclonal antibodies (mAbs), antibody-drug conjugates (ADCs), and therapeutic peptides. It provides high-resolution profiling of charge heterogeneity, glycosylation, and other post-translational modifications, streamlining development and regulatory submissions.</p></div><div><div><div><div><h4><strong>Proteomics and metabolomics</strong></h4></div></div></div></div><div><p>In proteomics, LC×LC enables deep peptide coverage post-digestion, advancing biomarker discovery. In metabolomics, it resolves thousands of metabolites in biological fluids, especially when integrated with high-resolution mass spectrometry.</p></div><div><div><div><div><h4><strong>Natural products and herbal extracts</strong></h4></div></div></div></div><div><p>2D-LC efficiently separates structurally similar phytochemicals, revealing trace bioactives crucial for drug discovery and nutraceuticals.</p></div><div><div><div><div><h4><strong>Food and beverage testing</strong></h4></div></div></div></div><div><p>2D-LC identifies flavor compounds, pesticides, mycotoxins, and additives, supporting quality control and safety standards in food sectors.</p></div><div><div><div><div><h4><strong>Petrochemical and environmental analysis</strong></h4></div></div></div></div><div><p>From petroleum fractions to persistent organic pollutants, LC×LC offers detailed compositional profiling for compliance and environmental protection.</p></div><div><div><div><div><h4><strong>Polymer characterization</strong></h4></div></div></div></div><div><p>2D-LC is used to examine polymer branching, blends, and molecular weight distributions.</p></div><div><div><div><div><h3><strong>Future Trends and Innovations in 2D-LC</strong></h3></div></div></div></div><div><p>Advancements in technology are rapidly transforming two-dimensional liquid chromatography into a more accessible, routine technique:</p><ul><li><strong>Miniaturization:</strong> Micro- and nano-LC systems reduce sample requirements and environmental impact while improving sensitivity and speed.</li><li><strong>Automated modulation:</strong> Innovations like active solvent modulation (ASM) and transfer modulation resolve solvent compatibility issues between dimensions, improving method robustness.</li><li><strong>Integration with mass spectrometry:</strong> LC×LC–MS is increasingly used in omics and pharmaceutical research for comprehensive structural elucidation.</li><li><strong>AI-driven data analysis:</strong> Artificial intelligence and machine learning algorithms simplify data interpretation and reduce analysis times.</li><li><strong>Green analytical chemistry:</strong> Solvent recycling and energy-efficient workflows are making 2D-LC more sustainable, aligning with global eco-conscious initiatives.</li><li><strong>3D chromatography:</strong> Research into three-dimensional chromatography hints at future developments, where even higher resolving power may become available.</li><li><strong>User-friendly software development:</strong> Efforts are underway to create more intuitive and compliant software solutions, making 2D-LC suitable for GMP laboratory environments.</li></ul></div>

2D-LC (Two-dimensional Liquid Chromatography): What, When and Why to Use it

<div><div><p><strong>For four days, Prague hosted experts in cellular metabolism, aging, and cancer. Researchers gathered at the Prague Metabolism and Signaling Symposium, organized by IOCB Boston, the U.S. branch of IOCB Prague. The symposium took place from June 24 to 27 at the National Library of Technology.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_3_b5de30c3fe.jpg" alt="IOCB Prague: Prague Metabolism and Signaling Symposium brought together leading researchers in metabolism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_3_b5de30c3fe.jpg 234w," sizes="100vw" width="2000" height="1335"></p></div></div><div><p>More than 30 speakers presented at the symposium, and nearly 200 researchers from leading European and American institutions – including <strong>MIT, Harvard, Stanford, Rockefeller University, the University of Basel, the Salk Institute for Biological Studies, Memorial Sloan Kettering Cancer Center, and the Francis Crick Institute</strong> – discussed the latest discoveries in cellular metabolism.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_6_1e1e9ce397.jpg" alt="IOCB Prague: Prague Metabolism and Signaling Symposium brought together leading researchers in metabolism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_6_1e1e9ce397.jpg 234w," sizes="100vw" width="2000" height="1335"></p><p>The first keynote lecture was delivered by <strong>Swiss scientist Michael N. Hall from the Biozentrum at the University of Basel</strong>. He is a pioneer in cell growth research and discovered the TOR protein, which plays a key role in cell growth regulation. The second keynote lecture was presented by <strong>British biologist Karen Vousden from the Francis Crick Institute in London</strong>, who focuses on how metabolic changes influence the behavior of cancer cells.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_2_dc03a0a75c.jpg" alt="IOCB Prague: Prague Metabolism and Signaling Symposium brought together leading researchers in metabolism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_2_dc03a0a75c.jpg 235w," sizes="100vw" width="2000" height="1331"></p><p>The symposium was chaired by <strong>David Sabatini of IOCB Prague and IOCB Boston</strong>, whose work has significantly contributed to the understanding of the mTOR signaling pathway, and <strong>Kateřina Rohlenová of the Institute of Biotechnology of the Czech Academy of Sciences</strong>, who investigates the metabolic relationships between cancer cells and healthy cells.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_8_148909a45d.jpg" alt="IOCB Prague: Prague Metabolism and Signaling Symposium brought together leading researchers in metabolism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_8_148909a45d.jpg 235w," sizes="100vw" width="2000" height="1331"></p><blockquote><p>More information about the program and speakers is available on the symposium <a target="_blank" rel="noopener noreferrer" href="https://praguemetabolismsymposium.com/"><strong>website</strong></a>.</p></blockquote><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_5_18fa8680f9.jpg" alt="IOCB Prague: Prague Metabolism and Signaling Symposium brought together leading researchers in metabolism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_5_18fa8680f9.jpg 235w," sizes="100vw" width="2000" height="1331"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_7_40ea24f626.jpg" alt="IOCB Prague: Prague Metabolism and Signaling Symposium brought together leading researchers in metabolism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_7_40ea24f626.jpg 235w," sizes="100vw" width="2000" height="1331"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_2a489e8665.jpg" alt="IOCB Prague: Prague Metabolism and Signaling Symposium brought together leading researchers in metabolism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_2a489e8665.jpg 235w," sizes="100vw" width="2000" height="1331"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_4_759ee05820.jpg" alt="IOCB Prague: Prague Metabolism and Signaling Symposium brought together leading researchers in metabolism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Prague_Metabolism_and_Signaling_Symposium_privitalo_svetovou_spicku_vyzkumu_metabolismu_4_759ee05820.jpg 235w," sizes="100vw" width="2000" height="1331"></p></div>

Prague Metabolism and Signaling Symposium brought together leading researchers in metabolism

<p><strong>Join us for an in-depth conversation with Holly Lee, Global Technical Marketing Specialist at SCIEX, as we explore the cutting edge of food and environmental safety testing. Holly shares her expertise on analyzing ultra-short chain PFAS like TFA, masked mycotoxins, pesticide residues, and the latest LC-MS/MS workflows transforming laboratory efficiency.</strong></p><p><strong>🎤 Holly Lee </strong>is a Global Technical Marketing Specialist for Food Applications at <strong>SCIEX</strong> with a PhD from the University of Toronto studying PFAS environmental processes. She has hands-on experience in analytical method development, LC-MS/MS analysis, and worked at the Ontario Ministry of the Environment before joining SCIEX.</p><h4><strong>In this episode of Concentrating on Chromatography, we discuss:</strong></h4><h5><strong>PFAS Analysis &amp; Challenges</strong></h5><ul><li>Why ultra-short chain PFAS compounds like trifluoroacetic acid (TFA) are among the toughest analytes to detect</li><li>Background contamination issues and how labs can minimize them</li><li>Column selection strategies and method development for comprehensive PFAS panels</li><li>Combining different stationary phase chemistries to broaden selectivity</li></ul><h5><strong>Mycotoxin Detection</strong></h5><ul><li>What are masked mycotoxins and why they evade conventional detection</li><li>Climate-driven changes in mycotoxin co-occurrence patterns</li><li>Achieving ultra-trace sensitivity for regulated limits in food matrices</li><li>EU regulations vs. global standards for mycotoxin testing</li></ul><h5><strong>Food Residue Testing Innovations</strong></h5><ul><li>Multi-residue pesticide methods covering 100+ compounds</li><li>Overcoming challenges with complex food matrices like tea, juice, and extracts</li><li>Veterinary drug residue analysis and validation strategies</li><li>The role of high-resolution mass spectrometry (HRMS) in resolving matrix interferences</li></ul><h5><strong>Laboratory Workflow Optimization</strong></h5><ul><li>Sample preparation strategies: when to use direct injection vs. SPE cleanup</li><li>Automation, AI, and machine learning for peak integration and data processing</li><li>Green chemistry practices and sustainability in food testing</li><li>Getting the most from your LC-MS/MS software features</li></ul><h5><strong>Holly's Background &amp; Insights</strong></h5><ul><li>Journey from studying PFAS fate at University of Toronto to food safety applications</li><li>Experience spanning Ontario Ministry of Environment, SCIEX R&amp;D, and global technical marketing</li><li>Emerging contaminants on the horizon, including microplastics analysis with LC-MS/MS</li><li>Partnership between SCIEX and Phenomenex for advancing chromatography solutions</li></ul><h5><strong>Resources Mentioned:</strong></h5><ul><li>NIST PFAS Interference List (PIL) database</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1530"><strong>Phenomenex Luna Omega PSC18 column</strong></a> for ultra-short chain PFAS</li><li>SCIEX 7500 and 7600 ZenoTOF systems</li><li>Science of the Total Environment journal article on microplastics</li></ul><p>Whether you're a food safety analyst, environmental chemist, or chromatography enthusiast, this conversation offers practical insights into method development, troubleshooting, and the future of analytical testing.</p><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

PFAS, Mycotoxins & Food Safety Testing with SCIEX's Holly Lee

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The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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Phenomenex Biozen SEC-3 LC columns

High inertness and particle consistency of both Biozen™ SEC columns drives greater reproducibility from injection-to-injection. 

<p><strong>High inertness and particle consistency </strong>of both Biozen™ SEC columns drives greater reproducibility from injection-to-injection. Combine this with the bioinert <strong>BioTi hardware</strong> and good aggregate recovery is no longer something that you’re missing out on.</p><p><strong>Biozen SEC columns provide:</strong></p><ul><li>High efficiency on both HPLC and UHPLC</li><li>Extreme inertness and robustness</li><li>Finely tuned high and low MS separation windows</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_SEC_3_3a6e232271.webp" alt="Phenomenex: Biozen SEC-3" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_SEC_3_3a6e232271.webp 245w," sizes="100vw" width="500" height="150"></p><ul><li><strong>Separation Mode:</strong> Gel Filtration Chromatography (GFC)</li><li><strong>Particle Size:</strong> 1.8</li><li><strong>Recommended Use:</strong> High molecular weight (HMW) separation range of 10 k – 700 kDa</li></ul><h3><strong>Phenomenex Biozen Biologics LC Columns</strong></h3><p>With a new titanium <strong>BioTi™</strong> biocompatible <strong>hardware</strong> to minimize priming, <strong>four particle platforms</strong> for optimal versatility and <strong>nine particle chemistries</strong> to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for <strong>protein analysis</strong> are seamlessly designed to bring peace of mind to your analysis of biologics through:</p><ul><li>Charge Variant Analysis</li><li>Aggregate Analysis</li><li>Peptide Mapping</li><li>Intact Mass Analysis</li><li>Intact and Subunit Analysis</li><li>Glycan Analysis</li><li>Peptide Quantitation</li><li>Drug Antibody Ratio (DAR)</li><li>Oligonucleotide Characterization</li></ul><h3><strong>Biocompatible Flow Path with BioTi™ Hardware</strong></h3><p>Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Bio_Ti_Hardware_991fb53395.jpg" alt="Phenomenex: BioTi Hardware" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Bio_Ti_Hardware_991fb53395.jpg 245w," sizes="100vw" width="1378" height="426"></p><h3><strong>4 Advanced Particle Platforms</strong></h3><p>All four of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg" alt="Phenomenex: Advanced Particle Platforms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg 245w," sizes="100vw" width="1439" height="868"></p>

Phenomenex Biozen SEC-2 LC columns

Biozen SEC columns are extremely inert, high density fully porous particle with high efficiency and low molecular weight separation range.

<p><strong>Biozen SEC columns</strong> are extremely inert, high density fully porous particle with high efficiency and low molecular weight (LMW) <strong>separation range</strong> of <strong>1 k – 450 kDa</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_SEC_3_3a6e232271.webp" alt="Phenomenex: Biozen SEC-2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_SEC_3_3a6e232271.webp 245w," sizes="100vw" width="500" height="150"></p><ul><li><strong>Separation Mode:</strong> Gel Filtration Chromatography (GFC)</li><li><strong>Particle Size:</strong> 1.8</li><li><strong>Recommended Use:</strong> Low molecular weight (LMW) separation range of 1 k – 450 kDa</li></ul><h3><strong>Phenomenex Biozen Biologics LC Columns</strong></h3><p>With a new titanium <strong>BioTi™</strong> biocompatible <strong>hardware</strong> to minimize priming, <strong>four particle platforms</strong> for optimal versatility and <strong>nine particle chemistries</strong> to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for <strong>protein analysis</strong> are seamlessly designed to bring peace of mind to your analysis of biologics through:</p><ul><li>Charge Variant Analysis</li><li>Aggregate Analysis</li><li>Peptide Mapping</li><li>Intact Mass Analysis</li><li>Intact and Subunit Analysis</li><li>Glycan Analysis</li><li>Peptide Quantitation</li><li>Drug Antibody Ratio (DAR)</li><li>Oligonucleotide Characterization</li></ul><h3><strong>Biocompatible Flow Path with BioTi™ Hardware</strong></h3><p>Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Bio_Ti_Hardware_991fb53395.jpg" alt="Phenomenex: BioTi Hardware" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Bio_Ti_Hardware_991fb53395.jpg 245w," sizes="100vw" width="1378" height="426"></p><h3><strong>4 Advanced Particle Platforms</strong></h3><p>All four of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg" alt="Phenomenex: Advanced Particle Platforms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg 245w," sizes="100vw" width="1439" height="868"></p>

Phenomenex Biozen dSEC-2 LC columns

Biozen dSEC columns use low-pore silica with a proprietary hydrophilic diol bond to prevent silica-protein interactions.

<p><strong>Advanced SEC Silica Particle Technology and Surface Chemistry for Characterizing Biomolecules</strong></p><p>The <strong>Biozen dSEC columns</strong> are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with <strong>protein samples</strong>.</p><ul><li>Exceptionally Robust, Pore Controlled SEC Particle</li><li>Extreme Stability and Exceptional Lifetime</li><li>Reproducible Separations</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_dsec_63af9af237.webp" alt="Phenomenex: Biozen-dSEC-2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_dsec_63af9af237.webp 245w," sizes="100vw" width="500" height="150"></p><ul><li><strong>Separation Mode:</strong> Gel Filtration Chromatography (GFC)</li><li><strong>Pore size</strong>: 200</li><li><strong>Particle Size:</strong> 1.8, 3</li><li><strong>Recommended Use:</strong> Analysis of monoclonal antibodies, biosimilars and other biomolecules by using specific molecular weights with improved column lifetimes and recovery</li></ul><h4><strong>Biozen SEC Proprietary Silica Particle Technology</strong></h4><p>The Biozen dSEC columns are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_SEC_Proprietary_Silica_Particle_Technology_502218b910.jpg" alt="Phenomenex: Biozen SEC Proprietary Silica Particle Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_SEC_Proprietary_Silica_Particle_Technology_502218b910.jpg 245w," sizes="100vw" width="1448" height="469"></p><h4><strong>Improved Column Lifetime and Performance Stability</strong></h4><p>Phenomenex’s optimized SEC column loading technology has significantly improved the overall packing density and silica distribution of the columns leading to improved chromatographic lifetime and stability.</p><h4><strong>New Standard for Platform SEC Methods</strong></h4><p>Whether IgG2 or IgG4 isotypes, bispecifics, or Fc-Fusions, dSEC-2 provides excellent separation and sample recovery for many different classes of antibodies and related recombinant proteins.</p><h3><strong>Phenomenex Biozen Biologics LC Columns</strong></h3><p>With a new titanium <strong>BioTi™</strong> biocompatible <strong>hardware</strong> to minimize priming, <strong>four particle platforms</strong> for optimal versatility and <strong>nine particle chemistries</strong> to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for <strong>protein analysis</strong> are seamlessly designed to bring peace of mind to your analysis of biologics through:</p><ul><li>Charge Variant Analysis</li><li>Aggregate Analysis</li><li>Peptide Mapping</li><li>Intact Mass Analysis</li><li>Intact and Subunit Analysis</li><li>Glycan Analysis</li><li>Peptide Quantitation</li><li>Drug Antibody Ratio (DAR)</li><li>Oligonucleotide Characterization</li></ul><h3><strong>Biocompatible Flow Path with BioTi™ Hardware</strong></h3><p>Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Bio_Ti_Hardware_991fb53395.jpg" alt="Phenomenex: BioTi Hardware" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Bio_Ti_Hardware_991fb53395.jpg 245w," sizes="100vw" width="1378" height="426"></p><h3><strong>4 Advanced Particle Platforms</strong></h3><p>All four of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg" alt="Phenomenex: Advanced Particle Platforms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg 245w," sizes="100vw" width="1439" height="868"></p>

Phenomenex Biozen dSEC-7 LC columns

dSEC-7 optimizes dimensions to save sample, speed up runs, and enhance resolution for AAVs and large biomolecule separations.

<p><strong>AAV and Large Biomolecule Aggregate Analysis​</strong></p><p><strong>Biozen dSEC-7 columns</strong> are packed with <strong>700 Å large pore silica</strong> coupled with a proprietary hydrophilic diol-type bonded surface chemistry that ensures silica surface interactions are mitigated. Designed with optimized column dimensions, dSEC-7 conserves valuable sample consumption, facilitates faster run times, and maximizes resolution for AAVs and large biomolecule separations.</p><p><strong>Robust and reliable aggregate analysis:</strong></p><ul><li>Baseline resolution of monomer/aggregate species through 700 Å pore size</li><li>Reduction in sample consumption by up to 90 % compared to traditional SEC columns</li><li>Reduction in method times by up to 50 % when compared to traditional SEC columns</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_dsec_63af9af237.webp" alt="Phenomenex: Biozen-dSEC-7" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_dsec_63af9af237.webp 245w," sizes="100vw" width="500" height="150"></p><ul><li><strong>Separation Mode:</strong> Gel Filtration Chromatography (GFC)</li><li><strong>Pore size</strong>: 700</li><li><strong>Recommended Use:</strong> Aggregate analysis of AAVs and other large biomolecules using specific molecular weights that conserves sample consumption, facilitates faster run times, and maximizes resolution.</li></ul><h4><strong>An SEC Column for AAV Aggregate Analysis</strong></h4><figure class="image"><img style="aspect-ratio:668/575;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_d_SEC_7_Column_b307c4217d.jpg" alt="Phenomenex: Biozen dSEC-7 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_d_SEC_7_Column_b307c4217d.jpg 182w," sizes="100vw" width="668" height="575"></figure><h4><strong>Biozen dSEC-7 Features</strong></h4><h5><strong>Improved Loading</strong></h5><ul><li>dSEC-7 addresses the challenge of aggregate analysis by offering accurate detection and separation with injections as low as 0.5 µL. Unlike traditional methods that demand large sample volumes, dSEC-7 conserves valuable samples, allowing for replicate analyses and ensuring availability for other CQA tests.</li></ul><h5><strong>Mobile Phase Flexibility</strong></h5><ul><li>dSEC-7’s particle properties facilitate robust separation of monomer from aggregate species, regardless of which mobile phase best suits your sample. This ensures reproducible results while accommodating unique sample demands, easing the burden of method condition optimization during development.</li></ul><h5><strong>Serotype Versatility</strong></h5><ul><li>With its robust performance and 700 Å pore size, dSEC-7 effectively resolves monomer from aggregate peaks across various AAV serotypes. This capability is crucial for evaluating appropriate serotype candidates, each varying in its unique tissue and cell tropisms, to ensure desired therapeutic outcomes are achieved.</li></ul><h5><strong>Instrument Agnostic Performance</strong></h5><ul><li>Despite differences in dwell volume and dispersion across various sites and departments, dSEC-7 consistently delivers robust and reproducible performance across commercially available LC instrumentation. This ensures reliable results regardless of variations in equipment, setup, or location.</li></ul><h5><strong>Outperforms Trending Solutions</strong></h5><ul><li>dSEC-7 excels in providing superior resolution over market alternatives, particularly when characterizing higher-level aggregates in AAV analysis. Unlike current market solutions with insufficient particle pore size, dSEC-7 ensures accurate and reproducible separation of AAV monomer from aggregates.</li></ul><h5><strong>Enhanced Detector Flexibility</strong></h5><ul><li>Conquer sample limitations using SEC-FLR for heightened sensitivity, SEC-MALS for precise molecular weight determination, or SEC-UV for routine analysis. Achieving comprehensive AAV characterization insights by transferring methods across detector technologies with unique benefits is now seamless.</li></ul><h3><strong>Phenomenex Biozen Biologics LC Columns</strong></h3><p>With a new titanium <strong>BioTi™</strong> biocompatible <strong>hardware</strong> to minimize priming, <strong>four particle platforms</strong> for optimal versatility and <strong>nine particle chemistries</strong> to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for <strong>protein analysis</strong> are seamlessly designed to bring peace of mind to your analysis of biologics through:</p><ul><li>Charge Variant Analysis</li><li>Aggregate Analysis</li><li>Peptide Mapping</li><li>Intact Mass Analysis</li><li>Intact and Subunit Analysis</li><li>Glycan Analysis</li><li>Peptide Quantitation</li><li>Drug Antibody Ratio (DAR)</li><li>Oligonucleotide Characterization</li></ul><h3><strong>Biocompatible Flow Path with BioTi™ Hardware</strong></h3><p>Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Bio_Ti_Hardware_991fb53395.jpg" alt="Phenomenex: BioTi Hardware" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Bio_Ti_Hardware_991fb53395.jpg 245w," sizes="100vw" width="1378" height="426"></p><h3><strong>4 Advanced Particle Platforms</strong></h3><p>All four of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg" alt="Phenomenex: Advanced Particle Platforms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg 245w," sizes="100vw" width="1439" height="868"></p>

Phenomenex Biozen dSEC-1 LC columns

Phenomenex Biozen dSEC-1 is a high-resolution SEC column for peptide therapeutics, delivering fast, reproducible aggregate analysis with improved peak shape and compatibility with SEC-MS and SEC-MALS.

<h4><strong>High-Resolution Aggregate Analysis for Peptide Therapeutics</strong></h4><p>Biozen dSEC-1 columns are engineered to meet the growing demand for aggregate analysis in peptide therapeutics and mid-sized biologics. Packed with 90 Å small pore silica and featuring inert diol-based surface chemistry, dSEC-1 delivers enhanced resolution for low and high molecular weight peptide aggregates. Its robust design ensures reproducibility and compatibility with advanced workflows like SEC-MS and SEC-MALS.</p><h5><strong>Robust Performance for Peptide Aggregate Analysis</strong></h5><ul><li>High resolution for low/high MW peptide aggregates (1–50 kDa)</li><li>Reproducible, and fast analysis with extended column lifetime</li><li>Improved peak asymmetry by up to 40%</li><li><strong>Recommended Use: </strong>Analysis of peptides, protein fragments, and small biologics with reduced analysis times and improved column lifetimes.</li></ul><h4><strong>Aggregate and Monomer Separation in Liraglutide Formulations</strong></h4><ul><li>Baseline resolution of monomer and two aggregates achieved in under 10 minutes</li><li>Exceptional reproducibility with retention time RSD ≤ 0.08% and peak area RSD ≤ 0.01%</li><li>Clear detection at low levels with S/N ratios of 13, 75, and 10,917 for aggregates and monomer</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_separation_in_liraglutide_formulations_3_b0d97cd01f.jpg" alt="Phenomenex: Aggregate and Monomer Separation in Liraglutide Formulations" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_separation_in_liraglutide_formulations_3_b0d97cd01f.jpg 245w," sizes="100vw" width="938" height="307"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_separation_in_liraglutide_formulations_2_035bfea3bc.jpg" alt="Phenomenex: Aggregate and Monomer Separation in Liraglutide Formulations" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_separation_in_liraglutide_formulations_2_035bfea3bc.jpg 245w," sizes="100vw" width="1700" height="1050"></p><h3><strong>Biozen SEC Proprietary Silica Particle Technology</strong></h3><p>The Biozen dSEC columns are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_SEC_Proprietary_Silica_Particle_Technology_502218b910.jpg" alt="Phenomenex: Biozen SEC Proprietary Silica Particle Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_SEC_Proprietary_Silica_Particle_Technology_502218b910.jpg 245w," sizes="100vw" width="1448" height="469"></p><h4><strong>Improved Column Lifetime and Performance Stability</strong></h4><p>Phenomenex’s optimized SEC column loading technology has significantly improved the overall packing density and silica distribution of the columns leading to improved chromatographic lifetime and stability.</p><h4><strong>New Standard for Platform SEC Methods</strong></h4><p>Whether IgG2 or IgG4 isotypes, bispecifics, or Fc-Fusions, dSEC-2 provides excellent separation and sample recovery for many different classes of antibodies and related recombinant proteins.</p><h3><strong>Phenomenex Biozen Biologics LC Columns</strong></h3><p>With a new titanium <strong>BioTi™</strong> biocompatible <strong>hardware</strong> to minimize priming, <strong>four particle platforms</strong> for optimal versatility and <strong>nine particle chemistries</strong> to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for <strong>protein analysis</strong> are seamlessly designed to bring peace of mind to your analysis of biologics through:</p><ul><li>Charge Variant Analysis</li><li>Aggregate Analysis</li><li>Peptide Mapping</li><li>Intact Mass Analysis</li><li>Intact and Subunit Analysis</li><li>Glycan Analysis</li><li>Peptide Quantitation</li><li>Drug Antibody Ratio (DAR)</li><li>Oligonucleotide Characterization</li></ul><h3><strong>Biocompatible Flow Path with BioTi™ Hardware</strong></h3><p>Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Bio_Ti_Hardware_991fb53395.jpg" alt="Phenomenex: BioTi Hardware" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Bio_Ti_Hardware_991fb53395.jpg 245w," sizes="100vw" width="1378" height="426"></p><h3><strong>4 Advanced Particle Platforms</strong></h3><p>All four of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg" alt="Phenomenex: Advanced Particle Platforms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg 245w," sizes="100vw" width="1439" height="868"></p>

Products from category GPC/SEC HPLC columns from Phenomenex distributed by Phenomenex - page 1

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