Meeting EU Drinking Water Standards: The Critical Role of Sample Prep in Trace Contaminant Analysis

Although modern agriculture needs to be based on accurate real-time data on soil, plant, and environmental conditions, affordable, sufficiently sensitive, and robust monitoring devices for this purpose are currently missing. According to scientists, low-cost sensors based on new smart materials and printing technologies offer a suitable solution. The results of multidisciplinary research thus far and proposals for the development of these devices have been presented by researchers from Czechia, Great Britain, Spain, and the USA in a review article in the prestigious journal <a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-68778-6#Abs1">Nature Communications</a>.“Sensor technologies for monitoring plant health are still in their infancy. Only simple sensors capable of monitoring basic parameters such as pH, soil moisture, or temperature are commonly available on the market. However, if farmers are to face current challenges, including climate change, and ensure sufficient food for humanity, they need much more effective tools that will enable them to monitor, for example, various pathogens, including bacteria and fungi. In our opinion, a suitable solution is a combination of new smart materials and printing for the production of inexpensive, durable, and scalable materials,” said the article’s first author, David Panáček from CATRIN, who worked on the study during his internship at Imperial College London.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg" alt="CATRIN: David Panáček" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg 208w," sizes="100vw" width="1536" height="1152">Sensors for monitoring plant health, for example, lag behind similar devices used in human medicine. According to the authors, advances in materials science, which will enable the development of scalable sensor technologies, play a key role in this potential shift. “Printing techniques, including inkjet printing, screen printing, aerosol printing, 3D printing, and direct laser writing, offer flexible ways to produce flexible sensors, large-area sensors, and sensors integrated directly into plants. The review article summarizes recent advances in printable low-dimensional materials for agricultural sensors, analyzes their physico-chemical properties in relation to sensor performance, and discusses key challenges and future opportunities requiring interdisciplinary integration,” said one of the corresponding authors, Michal Otyepka.The research brought together materials scientists, chemists, biologists, sensing experts, and printing technology specialists. Materials research and the use of graphene-based smart materials were led by scientists from CATRIN at Palacký University and VŠB-Technical University of Ostrava. Plant biologists from CATRIN contributed their knowledge of plant development, their responses to stress conditions, and the monitoring of their properties. Researchers from Imperial College London took care of the electronics, while representatives from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/36"> CEITEC – VUT</a> focused on printing technologies. The team also included leading experts in sensing from the Catalan Institute of Nanoscience and Nanotechnology in Barcelona, and Joseph Wang of the University of California San Diego, the “father of sensing and electrochemistry,” also contributed his extensive knowledge.“This is a groundbreaking study on this topic, which has managed to attract some truly big names in their respective fields. Last but not least, the study demonstrates that interdisciplinarity at CATRIN gives rise to completely new and promising research topics, such as plant sensing and its use for studying plant-environment interactions and smart agriculture,” added another corresponding author, Nuria De Diego.

Current sensors are not good enough. Scientists propose a new way to monitor plant health

In this episode of the Concentrating on Chromatography podcast, host David Oliva interviews Ainslie Chen about her cutting-edge research on hemoglobin variant testing using liquid chromatography-high resolution mass spectrometry (LC-HR-MS). Ainslie discusses her novel method for separating and identifying hemoglobin subunits with mass shifts less than 1 Da, overcoming critical limitations in conventional clinical assays.Hear firsthand how her team developed an LC-HR-MS workflow using a C4 reversed-phase column, tackled technical challenges (including why C18 columns fell short), and achieved unprecedented sensitivity for variant detection in both adult and neonatal samples. The conversation touches on the potential of mass spectrometry to revolutionize clinical diagnostics, the importance of reproducibility, barriers to adoption in routine labs, and how teamwork drives scientific innovation.Perfect for analytical chemists, clinical laboratorians, and anyone interested in the future of protein variant analysis, this episode also features Ainslie’s advice for early-career scientists and insights from her first ACS poster presentation.<h4>Key Highlights:</h4><ul><li>Motivation behind improving hemoglobinopathy testing &nbsp;</li><li>Technical advantages of LC-HR-MS over gene sequencing and CE &nbsp;</li><li>The crucial role of the C4 column in resolving challenging samples &nbsp;</li><li>Critical parameters for reproducible LC-HR-MS workflows &nbsp;</li><li>Barriers and outlook for clinical adoption of advanced mass spectrometry &nbsp;</li><li>The value of teamwork and mentorship in scientific research &nbsp;</li><li>Direct insights from recent ACS conference experiences</li></ul><h3>Video transcription</h3>In this interview, Ainslie Chen discusses her research on improving the detection and identification of hemoglobin variants using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS). The work addresses limitations of conventional hemoglobinopathy testing, which often struggles to resolve very small mass differences between closely related hemoglobin subunits. Because even a single amino-acid substitution can significantly alter hemoglobin structure and function, high analytical resolution is essential for accurate diagnosis of conditions such as anemia and sickle cell disease.Chen explains why her team selected LC-HRMS over established clinical techniques like capillary electrophoresis and gene sequencing. While sequencing offers excellent accuracy, it is time-consuming, costly, and requires extensive sample preparation, making it impractical for routine clinical workflows. Capillary electrophoresis enables subunit separation but lacks the resolving power needed to distinguish variants differing by less than one Dalton. In contrast, high-resolution mass spectrometry provides both structural specificity and sensitivity, enabling confident identification of subtle hemoglobin variants with minimal sample preparation.A key methodological insight from the project was the use of a C4 reversed-phase LC column instead of the more common C18 column. Chen describes how the shorter carbon chain and larger pore size of C4 columns improved protein elution and reduced denaturation, leading to more reproducible separations. Critical parameters for reproducibility included consistent mobile-phase composition (0.1% formic acid in water and acetonitrile), controlled temperature (60 °C for LC, ~320 °C capillary temperature), and stable chromatographic conditions.Beyond hemoglobin analysis, Chen highlights the broader potential of high-resolution mass spectrometry in proteoform analysis and clinical diagnostics, including emerging applications in cancer detection. She acknowledges current barriers to clinical adoption—such as energy demands and instrument infrastructure—but points to advances like miniaturized mass spectrometers as promising steps toward wider use. The interview concludes with advice for young scientists: don’t hesitate to reach out to mentors, embrace collaboration, and view analytical chemistry as a versatile tool that bridges medicine, biology, environmental science, and beyond.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Unlocking Hemoglobin Variant Detection: LC-HR-MS Breakthrough with Ainslie Chen

Article

Video

Environment

Scientific article

Interview

Science and research

The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Galochrom Chiral ION-QD HPLC columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan.

Chiral ion exchanger GEN-QD is highly efficient in the enantiomer resolution of acidic compounds.This product is now available in high quality and at a reasonable price at our company!To create our chiral sorbent, we use 3µm fully porous spherical silica particles, which provide greater efficiency than the original product.Additionally, our Galo-ION-QD-200 is recommended for biological applications due to its larger pore size, which improves the separation efficiency for large molecules.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QD_HPLC_kolony_ION_QD_selector_10dd9c7ee5.jpg" alt="Galochrom: Galochrom Chiral ION-QD selector."><h5>Premium Quality</h5>In every manufactured product, we guarantee the highest standards of quality.<h5>Cost-effective</h5>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.<h5>High-performance</h5>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.<h3>Ordering information</h3><blockquote>Stationary phase Chiral ION-QD is provided in these column types: analytical and preparative</blockquote><h4>Analytical Columns</h4><ul><li>Dimensions [mm]: 150 x 3</li><li>Porosity: [Å] 200</li><li>Cat. No.: ION02-A150</li><li>Price [€]: 1 300</li></ul><blockquote><h4>Get up to a 20% discount on your first purchase with us.</h4></blockquote><h3>Reach new heights</h3>Spend more time on testing and less time on troubleshooting<blockquote><h4>How can our unique columns help you succeed?</h4>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:<ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4>What makes Chiral <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1241">ION-QN</a> and ION-QD unique?</h4>Designed for stabilityThe manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376">During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"><h3>Achieve your results</h3>Increase your productivity with uniform, stable, and reproducible results<h4>High-Quality Silica</h4>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a result, our columns:<ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"><h4>Elution Order</h4>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers. Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545">The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:<ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

Galochrom Chiral ION-QN HPLC columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan. 

Our company is proud to offer the Galo ION-QN chiral ion exchanger, which is capable of efficiently separating enantiomers of acidic compounds.This product is now available in high quality and at a reasonable price at our company!To create our chiral sorbent, we use 3µm fully porous spherical silica particles, which provide greater efficiency than the original product.Additionally, our Galo-ION-QN-200 is recommended for biological applications due to its larger pore size, which improves the separation efficiency for large molecules.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QN_HPLC_kolony_ION_QN_selector_f3789cb2c1.jpg" alt="Galochrom: Galochrom Chiral ION-QN selector."><h5>Premium Quality</h5>In every manufactured product, we guarantee the highest standards of quality.<h5>Cost-effective</h5>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.<h5>High-performance</h5>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.<h3>Ordering information</h3><blockquote>Stationary phase Chiral ION-QN is provided in these column types: analytical and preparative</blockquote><h4>Analytical Columns</h4><ul><li>Dimensions [mm]: 150 x 3</li><li>Porosity: [Å] 200</li><li>Cat. No.: ION01-A150</li><li>Price [€]: 1 300</li></ul><blockquote><h4>Get up to a 20% discount on your first purchase with us.</h4></blockquote><h3>Reach new heights</h3>Spend more time on testing and less time on troubleshooting<blockquote><h4>How can our unique columns help you succeed?</h4>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:<ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4>What makes Chiral ION-QN and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1240">ION-QD</a> unique?</h4>Designed for stabilityThe manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376">During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"><h3>Achieve your results</h3>Increase your productivity with uniform, stable, and reproducible results<h4>High-Quality Silica</h4>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a result, our columns:<ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"><h4>Elution Order</h4>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers. Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545">The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:<ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

ECOM ION-QN and ION-QD Chiral HPLC Columns

 Weak Anion-Exchanger (WAX) stationary phases for reliable chiral separation of organic acids and amino acids. The most efficient approach to purify and resolve even the most troublesome enantiomeric mixtures.

Weak Anion-Exchanger (WAX) stationary phases for reliable chiral separation of organic acids and amino acids.&nbsp;We introduce our own generic series of weak anion-exchangers, based on tert-butyl-carbamoylated Cinchona alkaloids quinine (QN) and quinidine (QD). Our Chiral ION-QN and Chiral ION-QD columns contain a well-balanced pair of pseudoenantiomeric chiral selectors, designed to efficiently separate enantiomers of acidic compounds. These columns represent the most efficient approach to purify and resolve even the most troublesome enantiomeric mixtures.<h4>Applications</h4><ul><li>Chiral ION-QN and Chiral ION-QD are ideal for enantioseparations of chiral acidic compounds, including:</li><li>N-protected amino acids</li><li>Aminophosphonic &amp; Aminosulfonic acids</li><li>Lactic &amp; Thiolactic acids</li><li>Clenbuterol &amp; Thyroxine</li></ul><h4>Key Benefits</h4><ul><li>Provide better separation power and increased enantioselectivity (in comparison to other available alternatives)</li><li>Allow elegant switch of the elution order of enantiomers</li><li>Demonstrated high stability against all common HPLC solvents</li><li>Enable enhanced method development</li><li>Available at analytical and preparative scale</li></ul><h4>Columns</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Chiral_HPLC_Column_ION_QN_and_ION_QD_6_085a58fc5e.jpg" alt="ECOM: Chiral HPLC Column ION-QN and ION-QD" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Chiral_HPLC_Column_ION_QN_and_ION_QD_6_085a58fc5e.jpg 245w," sizes="100vw" width="1059" height="531"><h4>Features&nbsp;</h4><ul><li>Max. pressure: 5800 psi (400 bar)</li><li>Particle size: 3 or 5 μm</li><li>pH range: 2 to 9</li><li>Pore size: 120 Å</li><li>Surface area: 300 m²/g</li><li>Max. temperature: 60 °C</li><li>Packing material: Spherical, Fully Porous</li><li>Carbon load: 13.0 %</li><li>End-capped: Yes</li><li>Product line: ION</li></ul>The mobile phase composition (methanol-formic acid-ammonium formate) has been optimized for preparative applications to provide high column loadability, fast analyte elution, and good peak shape. Moreover, the buffer allows for simple product harvesting by solvent evaporation and subsequent ammonium formate sublimation 50 °C / 10 torr.The anion exchangers are perfect for the purification of organic acids and their chiral separation. They are very efficient in the chiral resolution of amino acids, but also other derivatives, such as amino phosphonic acids. Aminophosphonic acids are important precursors in the synthesis of phosphopeptidomimetics, which may potentially serve as new-generation pharmaceuticals.

Phenomenex Luna SCX HPLC Columns

The Luna SCX is a high performance silica phase with a benzene sulfonic acid bonded phase used for Strong Cation Exchange (SCX) chromatography.

The Luna SCX is a high performance silica phase with a benzene sulfonic acid bonded phase used for Strong Cation Exchange (SCX) chromatography.Key Features of the SCX Column:<ul><li>Extensive line of rugged particle sizes.</li><li>Easy method scalability from HPLC to preparative and bulk purification LC</li><li>Ultra-pure, metal-free silica (99.99% purity)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_SCX_HPLC_Column_4c175db0e0.webp" alt="Phenomenex: Luna SCX HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_SCX_HPLC_Column_4c175db0e0.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 0.55 % Sulfur Load, null %</li><li>Surface Area (m²/g): 400</li><li>Particle Size: 5, 10</li><li>Recommended Use: Strong cation exchange for positively charged compounds</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99% purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Develop Robust Methods</h4>Luna SCX columns provide excellent resolution and peak shape of basic‚ cationic compounds. Luna SCX columns contain a benzene sulfonic acid ligand providing ion-exchange‚ reversed phase‚ and aromatic interactions.<h4>Common Applications:</h4><ul><li>Cough and cold compounds, Raclopride, Sodium Acetate, Erythromycins</li></ul><blockquote>Stationary phase Luna SCX is provided in these column types: analytical, midbore and semi-preparative</blockquote><h5>Luna SCX HPLC MidBore, Analytical and Semi-preparative Columns are available with these parameters:</h5>MidBore Columns<ul><li>Column length (mm): 150</li><li>Column ID (mm): 3</li><li>Particle size (µm): 5</li></ul>Analytical Columns<ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 4.6</li><li>Particle size (µm): 5</li></ul>Semi-Prep Columns<ul><li>Column length (mm): 250</li><li>Column ID (mm): 10</li><li>Particle size (µm): 5</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Phenomenex Biozen WCX LC columns

Biozen WCX reproduces heterogeneity with high yield using bioinert titanium column hardware.

Biozen WCX was constructed with linear polycarboxylate chains grafted to monosized non-porous polymeric particles, to sinuously envelop and separate proteins from acidic and basic variants. With a highly tuned and controlled manufacturing process, Biozen WCX affords scientists a way to reproducibly characterize heterogeneity with an added advantage of excellent recovery using bioinert titanium column hardware.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_WCX_HPLC_Column_c54ed3343d.webp" alt="Phenomenex: Biozen WCX HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_WCX_HPLC_Column_c54ed3343d.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Separation Mode: Ion Exchange</li><li>Pore size: 0</li><li>Particle size: 6</li><li>Recommended Use: Separate proteins from acidic/basic variants</li></ul><h4>BioZen WCX Monosized Polymeric Non-Porous Particle</h4>Monosized particles grafted with linear polycarboxylate chains to envelop and separate proteins from acidic/basic variants.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_bio_Zen_WCX_Monosized_Polymeric_Non_Porous_Particle_edc90e05a7.jpg" alt="Phenomenex: bioZen WCX Monosized Polymeric Non-Porous Particle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_bio_Zen_WCX_Monosized_Polymeric_Non_Porous_Particle_edc90e05a7.jpg 245w," sizes="100vw" width="899" height="498"><h4>Minimize your priming and promote better recovery with our BioTi™ biocompatible hardware</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Bio_Ti_biocompatible_hardware_31656d98d6.jpg" alt="Phenomenex: BioTi™ biocompatible hardware" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Bio_Ti_biocompatible_hardware_31656d98d6.jpg 245w," sizes="100vw" width="1306" height="511"><h4>Monosized Particles for Optimal Consistency</h4>Our specially crafted polymeric particle is produced with outstanding precision so that you can be confident in increased performance and minimized variation between lots.<h4>Charge Variant Analysis</h4>BioZen WCX was crafted to consistently decipher between native protein variants that arise from PTMs within a therapeutics creation and development. The linear polycarboxylate chains grafted to monosized non-porous polymeric particles, envelop and separate proteins from acidic and basic protein variants. With such a highly. tuned and controlled manufacturing process, bioZen WCX media affords scientists a way to reproducibly characterize heterogeneity while taking advantage of excellent recovery through high particle inertness and bioinert titanium BioTi™ column hardware.<h4>Native Mode Mass Spectrometry with bioZen WCX</h4>Ion-exchange chromatography (IEX) is a technique employed in the characterization of biotherapeutics under nondenaturing conditions. Standard mobile phase conditions for these techniques are high in salt concentration, rendering them incompatible with mass spectrometry (MS) detection. Native MS represents a cutting edge approach to employ MS compatible mobile phases for non-denaturing IEX analysis. Observe and measure the non-covalent interactions and charge variants of proteins at high resolution using bioZen WCX.<h3>Phenomenex Biozen Biologics LC Columns</h3>With a new titanium BioTi™ biocompatible hardware to minimize priming, four particle platforms for optimal versatility and nine particle chemistries to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for protein analysis are seamlessly designed to bring peace of mind to your analysis of biologics through:<ul><li>Charge Variant Analysis</li><li>Aggregate Analysis</li><li>Peptide Mapping</li><li>Intact Mass Analysis</li><li>Intact and Subunit Analysis</li><li>Glycan Analysis</li><li>Peptide Quantitation</li><li>Drug Antibody Ratio (DAR)</li><li>Oligonucleotide Characterization</li></ul><h3>Biocompatible Flow Path with BioTi™ Hardware</h3>Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Bio_Ti_Hardware_991fb53395.jpg" alt="Phenomenex: BioTi Hardware" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Bio_Ti_Hardware_991fb53395.jpg 245w," sizes="100vw" width="1378" height="426"><h3>4 Advanced Particle Platforms</h3>All four of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg" alt="Phenomenex: Advanced Particle Platforms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg 245w," sizes="100vw" width="1439" height="868">

Products from category IEX HPLC columns focused on Consumables - page 3

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