<h3>A Dedicated Solution for Oligonucleotide Analysis</h3>Oligonucleotides have become key players in modern pharmaceutical and biological research, particularly in the development of highly targeted and personalized therapies. As increasing numbers of oligonucleotide-based drugs enter clinical trials or receive regulatory approval, analytical laboratories face growing demands for both throughput and data quality. These molecules present unique chromatographic challenges due to their long, highly polar structures and subtle variations in nucleobase sequence or functionalization.<h3>Sepapure oliGO – Designed for Oligonucleotide Separations</h3>Selecting the optimal chromatographic approach for oligonucleotide analysis can be complex. Many laboratories rely on ion-pair reversed-phase (IP-RP) chromatography because of its speed, robustness, and compatibility with mass spectrometry. However, commonly used ion-pair reagents can degrade conventional silica-based stationary phases.<a target="_blank" rel="noopener noreferrer" href="https://www.knauer.net/columns-oligonucleotide-columns">Sepapure oliGO</a> addresses this limitation through a hybrid silica matrix combined with specialized surface end-capping, ensuring resistance to aggressive IP-RP reagents. Its functionalized surface enables both strong reversed-phase interactions and controlled ionic contributions, effectively creating a mixed-mode separation mechanism. This design results in improved durability compared with standard mixed-mode columns, making Sepapure oliGO a robust choice for routine oligonucleotide workflows.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Sepapure_oli_GO_columns_with_laser_etched_column_information_23f545be81.jpg" alt="KNAUER: Figure 1 - Sepapure oliGO columns with laser-etched column information." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Sepapure_oli_GO_columns_with_laser_etched_column_information_23f545be81.jpg 223w," sizes="100vw" width="1186" height="828"><h3>Method Transfer and Comparative Performance</h3>In collaboration with BianoGMP GmbH, KNAUER’s application team evaluated Sepapure oliGO against a widely used reference column. The study compared oligonucleotides of varying lengths and purities, including a fully thiolated 43-mer DNA. Particular attention was paid to potential interactions between thiolated oligonucleotides and stainless-steel hardware, which have been reported to cause peak tailing.The results demonstrated that direct method transfer between the reference column and Sepapure oliGO was generally feasible without significant adjustment. Chromatographic resolution remained comparable, while retention times were slightly shorter on Sepapure oliGO. Furthermore, the anticipated benefits of expensive bioinert hardware were not confirmed under the tested conditions, suggesting that such effects may primarily influence very fast (U)HPLC separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Overlayed_chromatogram_of_crude_product_of_a_43mer_thiolate_oligonucleotide_7ae9613efd.jpg" alt="KNAUER: Figure 2: Overlayed chromatogram of crude product of a 43mer thiolate oligonucleotide. Light blue - reference column and dark blue – Sepapure oliGO." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Overlayed_chromatogram_of_crude_product_of_a_43mer_thiolate_oligonucleotide_7ae9613efd.jpg 245w," sizes="100vw" width="1223" height="453"><h3>Low Backpressure for Reliable Operation</h3>Sepapure oliGO can be applied effectively in both HPLC and (U)HPLC workflows. Its low operating backpressure minimizes risks associated with clogging, excessive system wear, and performance deterioration over time. Typical IP-RP separations using 3 µm particles generated pressures around 200 bar, enabling laboratories without ultra-high-pressure systems to perform high-quality oligonucleotide analysis.<h3>Best Practices for Column Performance</h3>To maintain optimal performance, users should observe recommended operating conditions, including a broad pH range of 1.5–10 and temperatures up to 60 °C. Employing a guard column can prevent coarse synthesis by-products from reaching the analytical column. If pressure increases are observed, backflushing at low flow rates can restore performance.When the system is idle, thorough flushing to remove salts is essential. For storage, the column should be kept in a 1:1 mixture of methanol–water or acetonitrile–water. Gradient transitions are recommended when switching between standard RP eluents and IP-RP reagents. Regeneration procedures developed for RP columns are also suitable for Sepapure oliGO.<h3>Beyond Analytical Separations</h3>For large-scale purification, ion-exchange chromatography (AEX) may offer advantages. Scalable preparative systems are available to support purification workflows, ranging from compact laboratory setups to pilot-scale platforms with flow rates up to 800 mL/min. Bioinert configurations can be integrated where required, allowing laboratories to tailor purification solutions to their specific oligonucleotide applications.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at sales@knauer.net. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at folmert@knauer.net&nbsp;</li></ul></blockquote>

Oligos Made Easy - Part 8: Sepapure oliGO

Visit the LabRulez booth at <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/871">analytica 2026</a> in Munich, 24. - 27. March 2026! You can find us in Hall A1, Booth A1.506. At the booth, you will be able to explore live demonstrations of how the LabRulez portals work and see selected analytical instruments from our pre-owned marketplace section.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg" alt="LabRulez at Analytica 2026: Hall A1 Floor Plan and Booth Location" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg 245w," sizes="100vw" width="1516" height="881"><h3>Would you like to learn how the LabRulez portals work?</h3>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/">LabRulezLCMS</a> ➡︎ <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/">LabRulezGCMS</a> ➡︎ <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/">LabRulezICPMS</a><ul><li>A unique online solution for your marketing</li><li>A targeted audience from the analytical chemistry community</li><li>Modern technologies and a unique concept</li><li>Design optimized for most smart devices – PC, mobile, and tablet</li><li>Advanced statistical tools fully available to users</li></ul><h3>Services Offered</h3><h4>News + Company Updates</h4><ul><li>Publish news related to your instrumentation, applications, services, events, job openings, and more</li><li>News is shared both on our portals and across our social media channels</li></ul><h4>Library – All Documents in One Place</h4><ul><li>Applications, posters, presentations, technical articles, brochures, specifications</li><li>Fast and advanced search capabilities</li><li>Over 32,000 documents currently available in our database</li></ul><h4>Webinars</h4><ul><li>Upcoming and on-demand webinars from the field of analytical chemistry</li><li>More than 5,500 webinars covering chromatography, mass spectrometry, and spectroscopy</li></ul><h4>Careers</h4><ul><li>Targeted job listings in analytical chemistry</li><li>Clear filtering by specialization or location</li><li>Increased visibility for job postings</li></ul><h4>Companies</h4><ul><li>Company profile with key information and contacts</li><li>Integration of all your activities on our portal: News, Library documents, Webinars, Upcoming Events, Careers, and mentions by partners or customers</li><li>Connection to your Products listed on LabRulez portals</li></ul><h4>Products and Services</h4><ul><li>Comprehensive overview of instrumental techniques and services</li><li>Targeting the analytical chemistry community and customers in the growing online environment</li></ul><h4>Marketplace</h4><ul><li>Buying and selling pre-owned analytical equipment</li><li>We purchase analytical instrumentation of all brands and ages</li><li>Sell your equipment through LabRulez</li><li>Request and purchase complete systems, modules, or spare parts with warranty and service</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg" alt="LabRulez at analytica 2026" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg 88w," sizes="100vw" width="1600" height="2844"><h3>What Will Be on Display at Our Booth?</h3>Come and explore selected spare parts and modules from our marketplace section. These include both new, tested, and pre-owned items.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar">LabRulezLCMS Marketplace</a></h4>Spare parts for LC/MS:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=378">Vacuum turbo pumps for LC/MS</a></li></ul>Spare parts for HPLC:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=397">Degassers for HPLC/UHPLC</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=368&amp;search=valve">Automatic valves for HPLC</a></li><li>HPLC components for Agilent 1100, 1200, and 1260 Infinity II series</li></ul>Consumables:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=377">HPLC lamps</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=48">HPLC columns</a></li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar">LabRulezGCMS Marketplace</a></h4>Spare parts for GC/MS:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=367&amp;search=turbo">Turbo pumps for GC/MS</a></li></ul>Spare parts for GC:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=366&amp;search=EPC">EPC modules</a></li></ul>Sample injection<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar/432">Autosampler 7693A for GC</a></li></ul>Consumables:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=7">GC columns </a>and selected accessories</li></ul>

LabRulez at analytica 2026

News from LabRulezLCMS Library - Week 13, 2026

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LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

ECOM PREPBOX A3L8E Chromatography Separation System

PREPBOX is a compact alternative to modular preparative systems. It is assembled according to the customer's wishes.

<div style="display:block;" aria-labelledby="tab_item-0">Complete preparative modular system with one basic gradient pump and optionally secondary pump for repetitive sampling or with injection valve. Also switching valve for&nbsp;<abbr title="Countercurrent Chromatography">CCC</abbr> applications. PDA four channel detector up to 800&nbsp;nm.&nbsp;It is assembled according to the customer's wishes. It is used for high-capacity systems or with centrifugal chromatography systems like in pharmaceutical purification of cannabis extracts (<abbr title="Cannabidiol">CBD</abbr>, <abbr title="Cannabinol">CBN</abbr>, <abbr title="Cannabigerol">CBG</abbr>, <abbr title="Cannabichromene">CBC</abbr>, <abbr title="Tetrahydrocannabinol">THC</abbr>...).ECOMAC SW installed at internal PC controls the system. Fractions are collected by means of&nbsp;10 position valve&nbsp; It is necessary to purchase an operating system for the PREPBOX&nbsp;- in most cases we recommend Windows OS&nbsp;(P/N:&nbsp;ABB90000) or Linux&nbsp;(on request). The system also supports the Clarity software. It is also possible to purchase a wireless mouse and keyboard set&nbsp;(P/N:&nbsp;EPC-0028) and a computer monitor&nbsp;(P/N:&nbsp;EPC-0027).<h5>TOY18DAD 800L Detector</h5>It is UV-VIS diode array detector, which allow measuring absorbance of four wavelengths in range of 200-800&nbsp;nm simultaneously in one cell just as scanning of whole spectra.<h5>ECP201L Preparative HPLC Pump</h5>Preparative pump suited for preparative liquid chromatography with very high pressure up to 15&nbsp;MPa&nbsp;at flow rate of 1000&nbsp;ml/min. Advantage of this pump is a very stable run even at low flow rates.<h5>ECP2300 Preparative Gradient Pump</h5>Preparative HPLC pump suited for&nbsp;preparative&nbsp;applications in liquid chromatography with a pressure up to 15 MPa at flow rate of 300&nbsp;ml/min.&nbsp;New sophisticated unit allows achieving a precise&nbsp;low-pressure gradient, i.e. gradual changes of solvent composition by mixing&nbsp;up to four liquids&nbsp;at the pump entry.<h3>Parameters</h3><ul><li>Interface: 5x USB, HDMI, 2X RS232, LAN, VGA</li><li>Power supply: 100 - 240 V AC</li><li>Power input: 490 W</li><li>Dimensions W x H x D: 405x490x525 mm (15.94x19.29x20.67 in)</li><li>Weight: 55 kg</li></ul></div>

ECOM COMPACT SEMI-PREPARATIVE SYSTEM (10 ml/min, ECS08)

A highly effective Compact semi-preparative system containing UV-VIS DAD detector, analytical gradient pump with degasser, automatic inject valve and fraction collector.

A highly effective Compact semi-preparative system containing&nbsp;UV-VIS DAD detector, analytical gradient pump with degasser, automatic inject valve and fraction collector. The detector allows absorbance measuring at four wavelengths at the same time or scanning through entire spectrum.The system is controlled by a built-in PC and touchscreen.A modular solution allows configuring the unit according to customer needs. This system contains a detector with a wavelength range of 200 –&nbsp; 800 nm, a pump with a flow rate up to 10 ml/min and a fraction collector.<h3>Main parts</h3><h5>TOY18DAD800L DETECTOR</h5>It is a UV-VIS diode array detector, which allows measuring the absorbance of&nbsp;four wavelengths in a range of 200–800 nm simultaneously in one cell&nbsp;just as scanning of whole spectra. Standardly is assembled with preparative cell PLCC07L, but other cells are available.<h5>ECF2096 FRACTION COLLECTOR</h5>Fractions are collected according to chosen method, which is simply created using a touch screen. Racks for 8, 21,40 ml tubes, 60 ml vials or 12 funnels, 30 mm.<h5>AUTOMATIC INJECT VALVE</h5>The valve is driven by a step motor and it is automatically switched by software. The system also allows manual sample injection by opening the column.<h5>SYSTEM PC INFO</h5>Installed industrial PC with Intel Atom 4 core processor. OS Linux, Touch screen 12,1“ 1024 x 786, SW QUEEN controls: gradient, flow rate, wavelengths, fractions collection etc. Remote administration is available. Possibility of measuring at four wavelengths at the same time or scanning through entire spectra. Furthermore, the possibility of displaying 3D data.&nbsp;<h5>ECP2010 ANALYTICAL GRADIENT PUMP</h5>Modified for the compact system,&nbsp;analytical gradient pump with degasser&nbsp;and with flow rate up to 10 ml/min&nbsp;(40 MPa, 5082 PSI).&nbsp;Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions. Four gradient valves allow changing mobile phases without bottles alternation.&nbsp;<h3>COMPACT PREPARATIVE SYSTEM &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</h3><ul><li>Interface: 3xUSB, 2xLAN, RS232</li><li>Power supply: 100 - 240 V AC</li><li>Power input: 400 W</li><li>Dimensions (W x H x D): 500 x 678 x 482 mm (19.69 x 26.69 x 18.98")</li><li>Weight: 55 kg (121.30 lb)</li></ul><h5>Detector</h5><ul><li>Wavelength range: 200 - 800 nm &nbsp; (256 elements on CCD)</li><li>Number of channels (Signals): 4</li><li>Scan: 200 - 800 nm,up to &nbsp;20 Hz, step 1 nm</li><li>Typical spectral half-width: 10 mn</li><li>Accuracy of adjustment / Reproducibility: ± 1 nm / ±0.5 nm</li><li>Noise level at test cell (254 nm, TC 0.75s): ± 5 x 10-5 AU</li><li>Drift at test cell (254 nm after 1 h): 1 x 10-3 AU/hr</li><li>Materials in contact with mobile phase: FEP; fused silica, stainless steel, PEEK</li><li>Time constant (T63): 0.5 s, 0.75 s, 1.0 s, 2.0 s, 4.0 s, 8.0 s, 16.0 s, 0.2 s, 0.1 s</li></ul><h5>Fraction collector</h5><ul><li>Available racks (two racks per unit)<ul><li>EC08 48 tubes of 8 ml</li><li>EC21 36 tubes of 21 ml</li><li>EC40 24 tubes of 40 ml</li><li>EC60 20 vials of 60 ml</li><li>F12A 12 funnels, 30 mm</li></ul></li><li>Maximum flow rate for tubes 8 ml: max. 50 ml/min</li><li>Maximum flow rate for tubes 21, 40 ml: max. 300 ml/min</li><li>Maximum flow rate for vials 60 ml: max. 300 ml/min</li><li>Maximum flow rate for funnels: max. 500 ml/min</li><li>Wetted materials: FEP, Tefzel®, PPS, KEL-F, PTFE, PEEK, SS 316, SIMAX glass</li><li>Input tubing: OD 1/8“ x ID 1/16“ FEP</li></ul><h5>Pump</h5><ul><li>Flow rate: 0.02 - 10.00 ml/min</li><li>Maximum operating pressure: 40 MPa (5082 PSI)</li><li>Repeatability of flow rate adjusting: ± 0.5 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Wetted materials: stainless steel, sapphire, KEL-F, seals*</li><li>Output capillary outer diameter: 1/16”</li><li>Input tubing outer diameter: 1/8”</li><li>Gradient valves control module - OPTIONALLY: Up to four valves</li></ul>

ECOM COMPACT PREPARATIVE SYSTEM (50 ml/min, ECS58)

A highly effective Compact preparative system containing UV-VIS DAD detector, analytical gradient pump with degasser, automatic inject valve and fraction collector.

A highly effective Compact preparative system containing&nbsp;UV-VIS DAD detector, analytical gradient pump with degasser, automatic inject valve and fraction collector. The detector allows absorbance measuring at four wavelengths at the same time or scanning through entire spectrum.The system is controlled by a built-in PC and touchscreen.A modular solution allows configuring the unit according to customer needs. This system contains a detector with a wavelength range of 200 –&nbsp; 800 nm, a pump with a flow rate up to 10 ml/min and a fraction collector.<h3>Main parts</h3><h5>TOY18DAD800L DETECTOR</h5>It is a UV-VIS diode array detector, which allows measuring the absorbance of&nbsp;four wavelengths in a range of 200–800 nm simultaneously in one cell&nbsp;just as scanning of whole spectra. Standardly is assembled with preparative cell PLCC07L, but other cells are available.<h5>ECF2096 FRACTION COLLECTOR</h5>Fractions are collected according to chosen method, which is simply created using a touch screen. Racks for 8, 21,40 ml tubes, 60 ml vials or 12 funnels, 30 mm.<h5>AUTOMATIC INJECT VALVE</h5>The valve is driven by a step motor and it is automatically switched by software. The system also allows manual sample injection by opening the column.<h5>SYSTEM PC INFO</h5>Installed industrial PC with Intel Atom 4 core processor. OS Linux, Touch screen 12,1“ 1024 x 786, SW QUEEN controls: gradient, flow rate, wavelengths, fractions collection etc. Remote administration is available. Possibility of measuring at four wavelengths at the same time or scanning through entire spectra. Furthermore, the possibility of displaying 3D data.&nbsp;<h5>ECP2050 PREPARATIVE GRADIENT PUMP</h5>Modified for the compact system,&nbsp;quaternary gradient pumps&nbsp;with flow rate up to&nbsp;50 ml/min&nbsp;(30 MPa, 4351 PSI).&nbsp;Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.<h3>COMPACT PREPARATIVE SYSTEM &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</h3><ul><li>Interface: 3xUSB, 2xLAN, RS232</li><li>Power supply: 100 - 240 V AC</li><li>Power input: 400 W</li><li>Dimensions (W x H x D): 500 x 678 x 482 mm (19.69 x 26.69 x 18.98")</li><li>Weight: 55 kg (121.30 lb)</li></ul><h5>Detector</h5><ul><li>Wavelength range: 200 - 800 nm &nbsp; (256 elements on CCD)</li><li>Number of channels (Signals): 4</li><li>Scan: 200 - 800 nm,up to &nbsp;20 Hz, step 1 nm</li><li>Typical spectral half-width: 10 mn</li><li>Accuracy of adjustment / Reproducibility: ± 1 nm / ±0.5 nm</li><li>Noise level at test cell (254 nm, TC 0.75s): ± 5 x 10-5 AU</li><li>Drift at test cell (254 nm after 1 h): 1 x 10-3 AU/hr</li><li>Materials in contact with mobile phase: FEP; fused silica, stainless steel, PEEK</li><li>Time constant (T63): 0.5 s, 0.75 s, 1.0 s, 2.0 s, 4.0 s, 8.0 s, 16.0 s, 0.2 s, 0.1 s</li></ul><h5>Fraction collector</h5><ul><li>Available racks (two racks per unit)<ul><li>EC08 48 tubes of 8 ml</li><li>EC21 36 tubes of 21 ml</li><li>EC40 24 tubes of 40 ml</li><li>EC60 20 vials of 60 ml</li><li>F12A 12 funnels, 30 mm</li></ul></li><li>Maximum flow rate for tubes 8 ml: max. 50 ml/min</li><li>Maximum flow rate for tubes 21, 40 ml: max. 300 ml/min</li><li>Maximum flow rate for vials 60 ml: max. 300 ml/min</li><li>Maximum flow rate for funnels: max. 500 ml/min</li><li>Wetted materials: FEP, Tefzel®, PPS, KEL-F, PTFE, PEEK, SS 316, SIMAX glass</li><li>Input tubing: OD 1/8“ x ID 1/16“ FEP</li></ul><h5>Pump</h5><ul><li>Flow rate: 0.1 – 50 ml/min</li><li>Maximum operating pressure: 30 MPa (4351 PSI)</li><li>Flow rate setting: 0.1 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 0.5 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Wetted materials: stainless steel, PEEK, TefzelTM, PE, ceramic, seals *</li><li>Output capillary outer diameter: 1/8”</li><li>Input tubing outer diameter: 1/8”</li><li>Gradient valves control module - OPTIONALLY: Up to six valves</li></ul>

ECOM COMPACT PREPARATIVE SYSTEM (250 ml/min, ECS28)

A highly effective Compact preparative system containing UV-VIS DAD detector, preparative gradient pump, an automatic inject valve, and a fraction collector. 

A highly effective Compact semi-preparative system containing&nbsp;UV-VIS DAD detector, preparative gradient pump, an automatic inject valve, and a fraction collector. The detector allows absorbance measuring at four wavelengths at the same time or scanning through entire spectrum.The system is controlled by a built-in PC and touchscreen.A modular solution allows configuring the unit according to customer needs. This system contains a detector with a wavelength range of 200 –&nbsp; 800 nm, a pump with a flow rate up to 10 ml/min and a fraction collector.<h3>Main parts</h3><h5>TOY18DAD800L DETECTOR</h5>It is a UV-VIS diode array detector, which allows measuring the absorbance of&nbsp;four wavelengths in a range of 200–800 nm simultaneously in one cell&nbsp;just as scanning of whole spectra. Standardly is assembled with preparative cell PLCC07L, but other cells are available.<h5>ECF2096 FRACTION COLLECTOR</h5>Fractions are collected according to chosen method, which is simply created using a touch screen. Racks for 8, 21,40 ml tubes, 60 ml vials or 12 funnels, 30 mm.<h5>AUTOMATIC INJECT VALVE</h5>The valve is driven by a step motor and it is automatically switched by software. The system also allows manual sample injection by opening the column.<h5>SYSTEM PC INFO</h5>Installed industrial PC with Intel Atom 4 core processor. OS Linux, Touch screen 12,1“ 1024 x 786, SW QUEEN controls: gradient, flow rate, wavelengths, fractions collection etc. Remote administration is available. Possibility of measuring at four wavelengths at the same time or scanning through entire spectra. Furthermore, the possibility of displaying 3D data.&nbsp;<h5>ECP2200 PREPARATIVE GRADIENT PUMP</h5>Modified for the compact system,&nbsp;quaternary gradient pumps&nbsp;with flow rate up to&nbsp;250 ml/min&nbsp;(30 MPa at 100 ml/min and then linearly down up to 15 MPa at 250 ml/min).&nbsp;Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.<h3>COMPACT PREPARATIVE SYSTEM &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</h3><ul><li>Interface: 3xUSB, 2xLAN, RS232</li><li>Power supply: 100 - 240 V AC</li><li>Power input: 400 W</li><li>Dimensions (W x H x D): 500 x 678 x 482 mm (19.69 x 26.69 x 18.98")</li><li>Weight: 55 kg (121.30 lb)</li></ul><h5>Detector</h5><ul><li>Wavelength range: 200 - 800 nm (256 elements on CCD)</li><li>Number of channels (Signals): 4</li><li>Scan: 200 - 800 nm,up to &nbsp;20 Hz, step 1 nm</li><li>Typical spectral half-width: 10 mn</li><li>Accuracy of adjustment / Reproducibility: ± 1 nm / ±0.5 nm</li><li>Noise level at test cell (254 nm, TC 0.75s): ± 5 x 10-5 AU</li><li>Drift at test cell (254 nm after 1 h): 1 x 10-3 AU/hr</li><li>Materials in contact with mobile phase: FEP; fused silica, stainless steel, PEEK</li><li>Time constant (T63): 0.5 s, 0.75 s, 1.0 s, 2.0 s, 4.0 s, 8.0 s, 16.0 s, 0.2 s, 0.1 s</li></ul><h5>Fraction collector</h5><ul><li>Available racks (two racks per unit)<ul><li>EC08 48 tubes of 8 ml</li><li>EC21 36 tubes of 21 ml</li><li>EC40 24 tubes of 40 ml</li><li>EC60 20 vials of 60 ml</li><li>F12A 12 funnels, 30 mm</li></ul></li><li>Maximum flow rate for tubes 8 ml: max. 50 ml/min</li><li>Maximum flow rate for tubes 21, 40 ml: max. 300 ml/min</li><li>Maximum flow rate for vials 60 ml: max. 300 ml/min</li><li>Maximum flow rate for funnels: max. 500 ml/min</li><li>Wetted materials: FEP, Tefzel®, PPS, KEL-F, PTFE, PEEK, SS 316, SIMAX glass</li><li>Input tubing: OD 1/8“ x ID 1/16“ FEP</li></ul><h5>Pump</h5><ul><li>Flow rate: 0.5 – 250 ml/min</li><li>Maximum operating pressure: 30 MPa (4351 PSI) up to 100 ml/min then &nbsp;linearly down up to</li><li>Flow rate setting: 0.1 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 1 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Wetted materials: stainless steel, PEEK, TefzelTM, PE, ceramic, seals *</li><li>Output capillary outer diameter: 1/8”</li><li>Input tubing outer diameter: 1/8”</li><li>Gradient valves control module - OPTIONALLY: Up to six valves</li></ul>

<div style="display:block;" aria-labelledby="tab_item-0">Complete preparative modular system with one basic gradient pump and optionally secondary pump for repetitive sampling or with injection valve. Also switching valve for&nbsp;<abbr title="Countercurrent Chromatography">CCC</abbr> applications. PDA four channel detector up to 800&nbsp;nm.&nbsp;It is assembled according to the customer's wishes. It is used for high-capacity systems or with centrifugal chromatography systems like in pharmaceutical purification of cannabis extracts (<abbr title="Cannabidiol">CBD</abbr>, <abbr title="Cannabinol">CBN</abbr>, <abbr title="Cannabigerol">CBG</abbr>, <abbr title="Cannabichromene">CBC</abbr>, <abbr title="Tetrahydrocannabinol">THC</abbr>...).ECOMAC SW installed at internal PC controls the system. Fractions are collected by means of&nbsp;10 position valve&nbsp; It is necessary to purchase an operating system for the PREPBOX&nbsp;- in most cases we recommend Windows OS&nbsp;(P/N:&nbsp;ABB90000) or Linux&nbsp;(on request). The system also supports the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a>. It is also possible to purchase a wireless mouse and keyboard set&nbsp;(P/N:&nbsp;EPC-0028) and a computer monitor&nbsp;(P/N:&nbsp;EPC-0027).<h5>TOY18DAD 800L Detector</h5>It is UV-VIS diode array detector, which allow measuring absorbance of four wavelengths in range of 200-800&nbsp;nm simultaneously in one cell just as scanning of whole spectra.<h5>ECP2300 Preparative Gradient Pump</h5>Two peparative HPLC pump suited for&nbsp;preparative&nbsp;applications in liquid chromatography with a pressure up to 15 MPa at flow rate of 300&nbsp;ml/min.&nbsp;New sophisticated unit allows achieving a precise&nbsp;low-pressure gradient, i.e. gradual changes of solvent composition by mixing&nbsp;up to four liquids&nbsp;at the pump entry.<h4>Complete preparative modular system with:</h4><ul><li>one basic gradient pump</li><li>for sampling secondary pump or injection valve</li><li>optionally switching valve for CCC</li><li>PDA detector up to 800 nm</li><li>10 positions valve for fractions collecting</li><li>PC board with ECOMAC SW</li></ul><h3>Parameters</h3><ul><li>Interface: 5x USB, HDMI, 2X RS232, LAN, VGA</li><li>Power supply: 100 - 240 V AC</li><li>Power input: 4900 W</li><li>Dimensions W x H x D: 405x490x525 mm (15.94x19.29x20.67 in)</li><li>Weight: 50 kg</li></ul></div>

ECOM ECS06 Gradient Analytical System

Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump, a gradient box, and an autosampler with/without cooling and heating, 40 MPa (5801 psi).

<div style="display:block;" aria-labelledby="tab_item-0">Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump, a gradient box, and an autosampler with/without cooling and heating, 40 MPa (5801 psi).The detector with eight channels in the range of 200-800 nm and a very low noise level also offers high-speed scanning up to 100 Hz. The units are communicated through RS232 or Ethernet (LAN). An additional PC A/D converter card is not required for this system. The system contains an autosampler and Clarity software. All units are supported in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a> from installation.A wireless mouse and keyboard set (P/N: EPC-0028), a PC (P/N: EPC-0026), and a computer monitor (P/N: EPC-0027) can be purchased with the system.<h3>Main parts</h3><h5>ECDA2800 UV-VIS PDA DETECTOR</h5>Analytical Diode Array Detector measures at eight wavelengths simultaneously or performs a full scan with a speed of up to 100 Hz. 3D support in Clarity SW. Noise level ±5 x 10-6 AU. Four analog outputs. Two special mathematical channels are available. AD 05 analytical cell is included.<h5>ECO2080L COLUMN OVEN WITH HEATING AND COOLING</h5>This column oven allows precise temperature stabilization in the range of 0-80°C. A big compartment room can accommodate up to 3 columns with lengths up to 30 cm. Peltier technology offers the possibility of heating and cooling independently from room temperature.<h5>ECP2010 ANALYTICAL HPLC PUMP</h5>This isocratic pump works with Gradient Box ECB2004B as a quaternary gradient pump. The flow rate range is 0.001-10.00 ml/min and the pressure limit is 40 MPa. Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.<h5>ECB2004B GRADIENT BOX WITH DEGASSER</h5>The box is suited for handling liquids in ECOM gradient systems. It accommodates a container for solvent bottles, a built-in high-efficiency vacuum degasser, and a four-way gradient valve. Up to four solvent lines may be degassed simultaneously by one unit. A four-way gradient valve is controlled from the pump ECP2010.<h5>AUTOSAMPLER AS96</h5>A reliable and precise autosampler for a wide range of applications in HPLC and LC-MS in variants with or without a cooling and heating module. State-of-the-art injection technology with fast injection and wash cycles. For 2x48 vials, max. pressure 40 MPa (5801 psi)<h5>CLARITY CHROMATOGRAPHY SOFTWARE</h5>This system is fully controlled by Clarity Chromatography software, which is also a part of delivery. Clarity software complies with CFR21GLP requirements.<h3>SYSTEM CONTAINS&nbsp;</h3>Pcs / Description (P/N)<ul><li>1x / ECDA2800 Analytical UV-VIS PDA Detector (ANW0000X)</li><li>1x /&nbsp;ECB2004B Gradient box with degasser (ABA0000X)</li><li>1x / ECP2010 Analytical HPLC pump (ACA0000X)</li><li>1x / ECO2080L Column oven with heating and cooling (AOA00004)</li><li>1x / Autosampler AS96 for 2x48 vials, 40 MPa, with or without heating and cooling module (ASA97010/ASA97000)</li><li>1x / Clarity – Station for 1 instrument (AP000C50)</li><li>1x / Clarity module for HPLC control (AP000A24)</li><li>1x / Clarity SW module for AS controlvAP000A26)</li><li>1x / Module for PDA data Clarity SW (AP000A29)</li><li>1x / Accessories for analyt. system series 2000 (ASA00180)</li><li>1x / Accessories for autosampler (ASA96180)</li></ul>Note: Column and computer not included. PC system has to be equipped for handling 3 serial RS232 or 1 Ethernet port at least. Please, check also <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a> requirements..<h3>Configuration options</h3><ul><li>ASA0600X - Gradient analytical system - incl. autosampler without cooling and heating, up to 40 MPa (5801 psi)</li><li>ASA06C0X - Gradient analytical system – incl. autosampler with cooling and heating, up to 40 MPa (5801 psi)</li></ul></div>

ECOM ECS07 Gradient Analytical System

Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump (60 MPa, 8702 psi), a gradient box, and an autosampler.

<div style="display:block;" aria-labelledby="tab_item-0">Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump, a gradient box, and an autosampler with/without cooling and heating, 60 MPa (8702 psi).The detector with eight channels in the range of 200-800 nm and a very low noise level also offers high-speed scanning up to 100 Hz. The units are communicated through RS232 or Ethernet (LAN). An additional PC A/D converter card is not required for this system. The system contains an autosampler and Clarity software. All units are supported in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a> from installation.A wireless mouse and keyboard set (P/N: EPC-0028), a PC (P/N: EPC-0026), and a computer monitor (P/N: EPC-0027) can be purchased with the system.<h3>Main parts</h3><h5>ECDA2800 UV-VIS PDA DETECTOR</h5>Analytical Diode Array Detector measures at eight wavelengths simultaneously or performs a full scan with a speed of up to 100 Hz. 3D support in Clarity SW. Noise level ±5 x 10-6 AU. Four analog outputs. Two special mathematical channels are available. AD 05 analytical cell is included.<h5>ECO2080L COLUMN OVEN WITH HEATING AND COOLING</h5>This column oven allows precise temperature stabilization in the range of 0-80°C. A big compartment room can accommodate up to 3 columns with lengths up to 30 cm. Peltier technology offers the possibility of heating and cooling independently from room temperature.&nbsp;<h5>ECP2010H ANALYTICAL HPLC PUMP</h5>This isocratic pump works with Gradient Box ECB2004B as a quaternary gradient pump. The flow rate range is 0.001-10.00 ml/min and the pressure limit is 60 MPa. Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.<h5>ECB2004B GRADIENT BOX WITH DEGASSER</h5>The box is suited for handling liquids in ECOM gradient systems. It accommodates a container for solvent bottles, a built-in high-efficiency vacuum degasser, and a four-way gradient valve. Up to four solvent lines may be degassed simultaneously by one unit. A four-way gradient valve is controlled from the pump ECP2010.<h5>AUTOSAMPLER AS96</h5>A reliable and precise autosampler for a wide range of applications in HPLC and LC-MS in variants with or without a cooling and heating module. State-of-the-art injection technology with fast injection and wash cycles. Max. pressure 60 MPa.<h5>CLARITY CHROMATOGRAPHY SOFTWARE</h5>This system is fully controlled by Clarity Chromatography software, which is also a part of delivery. Clarity software complies with CFR21GLP requirements.<h3>SYSTEM CONTAINS&nbsp;</h3>Pcs / Description (P/N)<ul><li>1x / ECDA2800 Analytical UV-VIS PDA Detector (ANW0000X)</li><li>1x /&nbsp;ECB2004B Gradient box with degasser (ABA0000X)</li><li>1x / ECP2010H Analytical HPLC pump (ACF0000X)</li><li>1x / ECO2080L Column oven with heating and cooling (AOA00004)</li><li>1x / Autosampler AS96 for 2x48 vials, 60 MPa, with or without heating and cooling module (ASA96000/ASA96010)</li><li>1x / Clarity – Station for 1 instrument (AP000C50)</li><li>1x / Clarity module for HPLC control (AP000A24)</li><li>1x / Clarity SW module for AS controlvAP000A26)</li><li>1x / Module for PDA data Clarity SW (AP000A29)</li><li>1x / Accessories for analyt. system series 2000 (ASA00180)</li><li>1x / Accessories for autosampler (ASA96180)</li></ul>Note: Column and computer not included. PC system has to be equipped for handling 3 serial RS232 or 1 Ethernet port at least. Please, check also <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a> requirements..<h3>Configuration options</h3><ul><li>ASA0700X - Gradient analytical system - incl. autosampler without cooling and heating, up to 60 MPa.</li><li>ASA07C0X - Gradient analytical system – incl. autosampler with cooling and heating, up to 60 MPa.</li></ul></div>

ECOM PREPSYSTEM - Chromatography Separation System

A complete flash/preparative/pilot scale/production modular system ready for customizable to the application needs.

We would like to introduce an ECOM product news – a complete flash/preparative/pilot scale/production modular system with an excellent price/performance ratio.<h3>Applications</h3>The system can be used e.g. in:<ul><li>high-capacity systems or with centrifugal liquid/liquid chromatography systems, like in pharmaceutical purification of plant extracts – e.g. cannabinoids (CBD, CBN, CBG, CBC, THC, etc.), peptides, and oligonucleotides, algae extracts, oleoresins, etc.,&nbsp;</li><li>Also in new molecule development or synthesis.</li></ul><h3>Main parts</h3><h4>Pumps</h4><ul><li>The device has 3 pumps, 2 main ones are intended for a "high" pressure gradient (parallel pump connection), and one of them has a 6-solvent selection valve.&nbsp;</li><li>An optional third pump is used for repetitive sampling of high-volume samples. The unit also has a loop sample injection valve and a switching valve for CCC/CPC applications (ascending/descending mode).&nbsp;</li><li>The unit's universal PDA detector measures absorbance at four wavelengths at the same time up to 800 nm.</li></ul><h4>Software</h4><ul><li>The ECOMAC software or <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a> (FDA 21 CFR Part 11 compliant) is internally installed in the PC controlling the system.</li></ul><h4>Customization</h4><ul><li>The unit is completely customizable to the application needs to meet any scale criteria–from small flash sample separation testing through pilot scale prediction to high-capacity production.</li><li>It could work in ranges of 10 ml/min.–6000 ml/min and it can be easily and automatically switched between these scales.</li></ul>

ECOM ECS23PC Quaternary Preparative Gradient System with Fraction Collector

A highly effective preparative system containing a UV-VIS DAD detector, a preparative pump, a gradient box with PC, and a fraction collector. The detector allows measuring at four wavelengths at the same time or an informative scan.

<div style="display:block;" aria-labelledby="tab_item-0">A highly effective preparative system containing a UV-VIS DAD detector, a preparative pump, a gradient box with PC, and a fraction collector. The detector allows measuring at four wavelengths at the same time or an informative scan.Communication is through RS232 or Ethernet (LAN). An additional PC A/D converter card is not required for this system. The system contains a manual injector and ECOMAC software which include drivers for all ECOM units.It is necessary to purchase an OS for the system - in most cases, we recommend OEM Windows OS (P/N: ABB90000) or Linux (on request). It is also possible to purchase a wireless mouse and keyboard set (P/N: EPC-0028) and a computer monitor (P/N: EPC-0027).<h3>Main parts</h3><h4>TOY18DAD H 600 DETECTOR</h4>A UV-VIS diode array detector, which allows measuring the absorbance of four wavelengths in the range of 200 – 600 nm simultaneously in one cell just as visualization of whole spectra. It is possible to use the detector in flash and preparative applications. Standardly is assembled preparative cell PLCC 15 but other cells are available.<h4>ECP2300 PREPARATIVE PUMP</h4>This isocratic pump works with Gradient Box ECB2007PC as a quaternary gradient pump. The flow rate range is 0.1-300 ml/min and the pressure limit at 15 MPa. Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.<h4>ECB2005PC GRADIENT BOX WITH PC, WITHOUT DEGASSER</h4>The box is suited for handling liquids in ECOM gradient systems. It accommodates a container for solvent bottles and a four-way gradient valve. A four-way gradient valve is controlled from the pump ECP2300. In this box is assembled an industrial PC board. This solution allows the installation of OS (Windows or Linux) and chromatography SW ECOMAC or Clarity.&nbsp;<h4>ECF2096 FRACTION COLLECTOR</h4>Fractions are collected according to a chosen method which can be created manually by keyboard, just as using ECOMAC software. Analog input allows collecting based on detector signal. Racks for 9, 21, and 40 ml tubes.<h4>ECOMAC CHROMATOGRAPHY SOFTWARE</h4>This system is fully controlled by ECOMAC software, which is also a part of delivery. It allows all units control, gradient creation, and fraction collecting.<h3>SYSTEM CONTAINS&nbsp;</h3>Pcs / Description (P/N)<ul><li>1x / TOY18DAD H 600 Four Channel Detector (ANW0000X)</li><li>1x / ECB2005PC Gradient Box with PC, without Degasser (ABBPC00X)</li><li>1x / ECP2300 Preparative Pump (ACE0000X)</li><li>1x / ECOMAC software (SA100000)</li><li>1x / ECF2096 Fraction collector (AFA0000X)</li><li>1x / Preparative injection valve 1/8”; 2ml loop (SY2032022)</li><li>1x / Accessory for prep. system series 2000 (ASP00180)</li></ul>Note: The system can be equipped with detectors with different wavelengths ranges and pumps with different flow rates from ECOM production.</div>

Products from category Liquid chromatographs distributed by ECOM spol. s r.o. - page 1

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