<p><strong>This workflow confirms and quantifies 31 drugs of abuse in oral fluid (including THC) in a 4.5‑minute LC run on the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149"><strong>Thermo Scientific Stellar mass spectrometer</strong></a><strong> using full scan + targeted MS2 (tMS2) with both HCD and CID fragmentation. With 1 µL injection and 0.1% formic acid in water/methanol mobile phases, the method supports ~13 samples/hour (up to ~320/day continuous operation) while maintaining confirmation performance via ion ratios.</strong></p><blockquote><h5><strong>Method at a glance</strong></h5><ul><li><strong>What matrix is tested?</strong> Oral fluid</li><li><strong>How many drugs?</strong> 31 drugs of abuse (panel includes THC)</li><li><strong>Run time?</strong> 4.5 minutes</li><li><strong>Throughput?</strong> ~13 samples/hour → up to ~320 samples/day (24‑hour operation)</li><li><strong>Injection volume?</strong> 1 µL</li><li><strong>Mobile phases?</strong> 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B)</li><li><strong>MS acquisition?</strong> Full scan + targeted MS2 (tMS2)</li><li><strong>Fragmentation?</strong> Compound‑optimized <strong>HCD</strong> and <strong>CID</strong> in the same targeted list</li><li><strong>Confirmation strategy?</strong> Ion ratios with ±20% tolerance (typical confirmatory approach)</li></ul></blockquote><h3><strong>Why oral fluid—and why speed matters</strong></h3><p>Oral fluid is attractive for clinical and forensic toxicology because collection is simple and the matrix aligns well with high‑volume workflows (workplace/occupational, roadside, and other settings). The challenge is building a method that’s simultaneously:</p><ul><li><strong>Broad enough</strong> for today’s drug panels</li><li><strong>Sensitive enough</strong> to reach required cutoffs</li><li><strong>Fast enough</strong> to keep sample queues under control</li><li><strong>Confirmation‑ready</strong> (consistent product ions and ion ratios)</li></ul><p>A <strong>4.5‑minute</strong> method is a practical lever here: compress runtime, raise throughput, and still keep confirmation quality by optimizing MS/MS conditions per compound.</p><h3><strong>The LC method</strong></h3><p>A short, efficient separation is the foundation of the workflow:</p><ul><li><strong>UHPLC system:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744"><strong>Thermo Scientific Vanquish Horizon UHPLC</strong></a></li><li><strong>Column:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/424"><strong>Thermo Scientific Accucore Biphenyl</strong></a>, 50 × 2.1 mm, 2.6 µm</li><li><strong>Mobile phase A:</strong> 0.1% formic acid in water</li><li><strong>Mobile phase B:</strong> 0.1% formic acid in methanol</li><li><strong>Injection volume:</strong> <strong>1 µL</strong></li><li><strong>Gradient runtime:</strong> <strong>4.5 minutes</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_fd3f3e8ea4.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 1: Thermo Scientific Vanquish Horizon UHPLC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_fd3f3e8ea4.jpg 120w," sizes="100vw" width="747" height="969"></p><h3><strong>The MS method (Full scan + targeted MS<sup>2</sup> with HCD + CID)</strong></h3><h5><strong>Acquisition approach</strong></h5><p>This method uses a “two‑layer” strategy for quantitation + confirmation:</p><ol><li><strong>Full scan</strong> (broad coverage, context, quantitative support)</li><li><strong>Targeted MS<sup>2</sup> (tMS2)</strong> (confirmation‑focused product ions, ion ratios)</li></ol><h5><strong>The key differentiator: HCD and CID in one targeted list</strong></h5><p>Instead of forcing every analyte into a single fragmentation style, the method uses both:</p><ul><li><strong>HCD</strong> (beam‑type fragmentation)</li><li><strong>CID</strong> (resonance‑type fragmentation)</li></ul><h5><strong>Why that improves method development:</strong></h5><p>Drugs of abuse span multiple chemistries. A single collision approach often works “okay” for most compounds—but one or two analytes can become persistent problems (weak qualifiers, unstable ion ratios, excess noise). Having both <strong>HCD and CID</strong> available in one method lets you optimize per compound and move faster.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_3_e4a95651a2.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 2: Thermo Scientific Stellar mass spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_3_e4a95651a2.jpg 245w," sizes="100vw" width="645" height="377"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_5_88276eec6a.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 3: Full scan &amp; targeted MS2 methods, HCD and collision energies used." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_5_88276eec6a.jpg 245w," sizes="100vw" width="768" height="279"></p><h3><strong>Why CID + HCD flexibility improves confirmation</strong></h3><p>In this workflow, <strong>HCD</strong> performed well for most compounds. But for <strong>buprenorphine</strong>, HCD produced one dominant product ion with additional low m/z noise and noticeable residual precursor. Using <strong>CID</strong> generated two high‑intensity product ions and delivered <strong>consistent ion ratios</strong> across the calibration range.</p><p>When your confirmation hinges on stable ion ratios, the ability to switch fragmentation mode (not just collision energy) is a method‑development advantage—especially for “problem analytes.”</p><h3><strong>Controllable ionization</strong></h3><p>Oral fluid methods often deal with limited sample volume and matrix effects. This workflow pairs low injection volume with ionization control using the Thermo Scientific OptaMax Plus HESI source.</p><ul><li>The OptaMax Plus HESI source supports high probe temperature capability (up to 650 °C).</li><li>In this study, a vaporizer temperature of 500 °C was used to help achieve sensitivity and improve relative peak areas across drugs of abuse.</li></ul><p><strong>Why this matters:</strong> If you’re injecting only 1 µL, ionization stability and efficiency can be the difference between “meets cutoff” and “misses at LOQ.”</p><h3><strong>Sample preparation</strong></h3><p>Oral fluid panels become more challenging when THC is included because most drugs of abuse are basic, while THC is largely neutral, and that can stress extraction chemistry.</p><p>In this workflow, oral fluid calibrators and samples were extracted using a mixed‑mode approach designed to recover THC alongside other drugs:</p><ul><li>Calibrators prepared across eight levels (broad dynamic range)</li><li>Extraction performed using mixed‑mode chemistry tips (SCX/WAX) in a dispersive SPE‑style workflow</li><li>Steps automated on a liquid handling platform to support high‑throughput consistency</li><li>Samples dried and reconstituted prior to LC‑MS analysis</li><li>Only 1 µL injected per run</li></ul><p>Even if your lab uses different automation or consumables, the method structure (fast LC + full scan + targeted MS2 with dual fragmentation) transfers well.</p><blockquote><h5><strong>Throughput math</strong></h5><ul><li><strong>4.5 minutes per injection</strong> → 60 / 4.5 = <strong>13.3 injections/hour</strong></li><li>That’s <strong>~13 samples/hour</strong> in typical phrasing</li><li>Over 24 hours: 13.3 × 24 = <strong>~320 samples/day</strong> (continuous operation)</li></ul><p>If you run a single shift, just scale accordingly (e.g., ~106/day for an 8‑hour shift, excluding QC and overhead).</p></blockquote><h3>Performance summary</h3><h5><strong>Sensitivity and linearity</strong></h5><ul><li><strong>Linearity</strong> from LOQs as low as <strong>0.25 ng/mL</strong> up to <strong>1,000 ng/mL</strong> (panel-dependent)</li><li>For THC specifically: <strong>LOQ at 1 ng/mL</strong>, with a dynamic range extending to <strong>1,000 ng/mL</strong> in this evaluation</li><li>Across the panel, LOQs were reported as <strong>lower than recommended confirmation cutoffs</strong> referenced in SAMHSA guidance and NSC Tier 1 cutoffs (study-specific outcome)</li></ul><h5><strong>Confirmation via ion ratios</strong></h5><ul><li>Ion ratios evaluated using <strong>±20% tolerance</strong> (typical confirmatory acceptance framework)</li><li>The workflow produced <strong>consistent ion ratios across calibrator levels</strong> for the full panel in this evaluation</li></ul><h5><strong>Case sample comparison (real-world relevance)</strong></h5><p>When compared against a validated triple quadrupole method for case study samples, the Stellar MS workflow:</p><ul><li>Confirmed the positive hits observed by the reference method</li><li>Reported a few additional positives for select analytes, attributed to lower LOQs</li><li>Highlighted an operational efficiency point: <strong>1 µL injection</strong> in this method versus <strong>20 µL injection</strong> in the compared method</li></ul><h3><strong>FAQ</strong></h3><div><div><ol><li><strong>How long does the LC‑MS run take for the 31‑drug oral fluid panel?</strong><br><strong>4.5 minutes per injection</strong> using a short UHPLC gradient.<br>&nbsp;</li><li><strong>How many drugs of abuse can Stellar MS detect in this workflow?</strong><br><strong>31 drugs of abuse</strong>, including <strong>THC</strong>, using full scan + targeted MS2.<br>&nbsp;</li><li><strong>What MS/MS approach is used?</strong><br>A combined strategy: <strong>full scan</strong> plus <strong>targeted MS2 (tMS2)</strong> for confirmation.<br>&nbsp;</li><li><strong>Why use both HCD and CID?</strong><br>Because some analytes fragment better—and produce more stable confirmation ions/ion ratios—under one mode versus the other. Using both increases flexibility and speeds method optimization.<br>&nbsp;</li><li><strong>What injection volume is used?</strong><br><strong>1 µL</strong> of extracted sample.<br>&nbsp;</li><li><strong>What mobile phases are used?</strong><br><strong>0.1% formic acid in water (A)</strong> and <strong>0.1% formic acid in methanol (B)</strong>.<br>&nbsp;</li><li><strong>How many samples can you run per day?</strong><br>At 4.5 minutes/injection, throughput is <strong>~13 samples/hour</strong> or <strong>up to ~320/day</strong> with continuous operation (actual throughput depends on QC and scheduling).</li></ol></div></div><h3><strong>What makes this workflow “easy” to develop on Stellar MS</strong></h3><p>Here’s the short list of features that directly reduce method‑development friction:</p><ul><li><strong>HCD or CID</strong> in one method → optimize per compound, not compromise</li><li><strong>Fast MS2 acquisition speeds</strong> (up to 140 Hz for MS2) → more data points across narrow peaks</li><li><strong>Fast polarity switching</strong> (true 5 ms) → supports mixed panels efficiently</li><li><strong>Adaptive RT</strong> (real‑time retention time window adjustment) → helps reduce reinjections when retention times drift (e.g., column aging) and supports method transfer from longer to shorter gradients</li><li><strong>OptaMax Plus HESI</strong> source control → supports sensitivity at low injection volumes</li></ul><p><i>If you’re building a fast oral fluid confirmation workflow—or updating an existing DOA panel—this 4.5‑minute method shows how Stellar MS can combine <strong>speed, confirmation-ready MS2, and fragmentation flexibility (HCD + CID)</strong> in a single targeted workflow. Reach out to learn how to adapt the targeted list, collision settings, and scheduling strategy to your lab’s cutoffs and throughput goals.</i></p><blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a><strong>&nbsp;</strong>#MassSpectrometry #OralFluid #DrugsOfAbuse #MethodDevelopment #HighThroughput</p></blockquote>

4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS

<h2><a target="_blank" rel="noopener noreferrer" href="https://www.isc2026.org/"><strong>ISC 2026</strong></a></h2><p><strong>The </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/314"><strong>International Symposium on Chromatography (ISC)</strong></a><strong> represents the oldest conference series on separation science. ISC symposia have been organised since 1956 in each even year. ISC is one of the premier meetings series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.</strong></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/644"><strong>35th International Symposium on Chromatography</strong></a></h4><p>📆<strong>6. - 10. September 2026</strong></p><p>📍<strong>Prague, Czech Republic</strong></p><h3><strong>REGISTRATION</strong></h3><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://www.czech-in.org/cmPortalV15/Account/Login?ReturnUrl=%2FcmPortalV15%2FPortal%2FISC26%2Fnormal"><strong>Register HERE for the ISC 2026</strong></a></p><p><strong>Late Registration until 25 August 2026</strong></p></blockquote><h3><strong>INDUSTRY ACTIVITIES</strong></h3><h4><strong>Activity 1: Discussion Session: Industry &amp; Education</strong></h4><ul><li>Monday, 7 September 2026 | 16:30-17:50</li><li><strong>Place:</strong> Club A</li><li><strong>Moderators:</strong> Tony Edge and Deirdre Cabooter&nbsp;</li></ul><h5><strong>Concept:</strong></h5><p>This discussion session will explore the relationship between education in separation science in academia and the expectations of industry, focusing on how universities and companies can better collaborate to prepare graduates for successful careers in analytical laboratories and R&amp;D environments.<br>The goal of the session is to provide perspectives from both academia and industry on how education in chromatography and related separation techniques can evolve to better reflect current technological, methodological, and regulatory challenges.</p><h5><strong>Discussion Topics:</strong></h5><ul><li><strong>Bridging academia and industry: </strong>how both sectors perceive current cooperation and where improvements are possible&nbsp;</li><li><strong>Graduate preparedness:</strong> which skills and competencies are often missing when entering industrial roles, and in which areas graduates typically excel&nbsp;</li><li><strong>Approaches to teaching separation science:</strong> how chromatography and related analytical techniques are taught across different universities and regions&nbsp;</li><li><strong>Industry support for education: </strong>examples of collaboration such as internships, industrial placements, joint teaching activities, or access to instrumentation&nbsp;</li><li><strong>Experiences with academia–industry partnerships: </strong>what models of cooperation have proven effective in practice&nbsp;</li><li><strong>Future perspectives</strong>: how education in separation science should adapt to emerging trends such as automation, digitalization, sustainability, and regulatory requirements&nbsp;</li></ul><h5><strong>Format:</strong></h5><p>Moderated panel discussion with short introductory remarks from invited speakers representing both academia and industry, followed by an interactive discussion with the audience.</p><h5><strong>Panelists:</strong></h5><ul><li>Shimadzu&nbsp;</li><li>Novartis&nbsp;</li><li>Jelle De Vos, RIC Group&nbsp;</li><li>Davy Guillarme, University of Geneva&nbsp;</li><li>Caterina Temporini, University of Pavia&nbsp;</li></ul><h4><strong>Activity 2: Career Presentation&nbsp;</strong></h4><ul><li>Monday, 7 September 2026 | 18:00-19:00</li><li><strong>Place: </strong>Club A</li><li><strong>Moderators: </strong>Adrian Clarke and Martina Catani</li></ul><h5><strong>Concept:</strong></h5><p>This session will provide an overview of career opportunities in separation science across industry, highlighting the wide range of professional pathways available to graduates and experienced scientists working in chromatography and related analytical disciplines.</p><p>Participating companies will introduce their organizations, present typical roles involving separation techniques, and share insights into the skills and competencies they seek in candidates. The session is intended to support students, early-career researchers, and experienced professionals interested in exploring career options in industry sectors.</p><h5><strong>Discussion Topics:&nbsp;</strong></h5><ul><li>Overview of career paths involving chromatography and separation science&nbsp;</li><li>Entry-level opportunities for graduates and PhD holders&nbsp;</li><li>Positions for experienced scientists and technical specialists&nbsp;</li><li>Roles in R&amp;D, analytical development, quality control, regulatory science, application support, and instrument development&nbsp;</li><li>Skills and competencies valued by employers in separation science&nbsp;</li><li>Internships, graduate programs, and early-career development opportunities&nbsp;</li><li>Mobility between academia and industry&nbsp;</li></ul><h5><strong>Format:</strong></h5><p>Short presentation providing:</p><ul><li>overview of different carrier paths&nbsp;</li><li>introduction to the company and its activities related to separation science&nbsp;</li><li>examples of typical roles and career progression paths&nbsp;</li><li>information about current or expected hiring needs&nbsp;</li><li>advice for candidates interested in pursuing careers in industry&nbsp;</li><li>the session should include time for informal discussion, allowing participants to interact with company representatives and learn more about specific opportunities.&nbsp;</li></ul><h4><strong>Activity 3: Job Fair</strong></h4><ul><li>Monday, 7 September and Tuesday, 8 September 2026 | 12:30-14:00</li><li><strong>Place: </strong>Club A</li><li><strong>Main organizer:</strong> Adrian Clarke</li></ul><h5><strong>Concept:</strong></h5><p>The ISC 2026 Job Fair provides a structured opportunity for direct interaction between companies active in separation science and conference participants seeking career opportunities. The session is designed to facilitate efficient, focused conversations between employers and candidates interested in positions related to chromatography and analytical science.</p><p>Participating candidates will submit their CV in advance allowing companies to review profiles prior to the event and identify potential matches based on expertise, experience, and career interests.</p><h5><strong>Format:</strong></h5><p>The Job Fair will follow a structured rotation format:</p><ul><li>Each participating company will be assigned a dedicated table&nbsp;</li><li>One company representative will meet candidates individually&nbsp;</li><li>Each candidate will have 5 minutes per meeting to introduce their background and interests&nbsp;</li><li>Participants will rotate between companies at timed intervals&nbsp;</li><li>Session timing will be managed by the organizer, with a signal indicating when to move to the next meeting&nbsp;</li></ul><h5><strong>Candidates:</strong></h5><p>Participants interested in joining the Job Fair are asked to:</p><ul><li>submit their CV before August 23rd &nbsp;</li><li>indicate areas of expertise and career interests&nbsp;</li><li>specify whether they are seeking internships, entry-level roles, or experienced positions&nbsp;</li><li>specify if you cannot be available on a certain day e.g. owing to poster presentation&nbsp;</li><li>send these materials to: <a href="mailto:careers@isc2026.org">careers@isc2026.org</a>&nbsp;</li></ul><h5><strong>Companies:</strong></h5><p>Confirmed participating companies to date include:</p><ul><li>Novartis&nbsp;</li><li>RIC Group&nbsp;</li><li>Shimadzu&nbsp;</li><li>further companies to be added&nbsp;</li></ul><p>Potential employers please register your interest to <a href="mailto:careers@isc2026.org">careers@isc2026.org</a> to participate in the speed-dating (first come first served basis).</p><p>“<strong>Jobs position</strong>” board will be available in the Careers fair in the foyer in the symposium venue and attendees can visit the company booths in the exhibition hall or discuss with recruiting companies during the conference.</p><blockquote><p>Recruiters please send an email with your open positions to <a href="careers@isc2026.org">careers@isc2026.org</a> by the <strong>23rd August</strong> and bring the job adverts to post on the jobs board (there will also be the possibility to upload the open positions to the Symposium app).</p></blockquote><h4><strong>Activity 4: Discussion Session: Artificial Intelligence in Separation Science: From Concept to Practice</strong></h4><ul><li>Tuesday, 8 September 2026 | 16:30-18:00</li><li>Place: Club A</li><li><strong>Moderators:</strong> Bob Pirok</li></ul><h5><strong>Concept:</strong></h5><p>Artificial intelligence (AI) and data-driven approaches are increasingly influencing analytical chemistry, offering new possibilities for method development, data interpretation, process optimization, and decision support. This discussion session will explore how AI is currently being applied in separation science, with a particular focus on practical implementation in industry environments.</p><p>The session aims to provide a realistic overview of the current state of AI adoption, highlighting both successful applications and remaining challenges. Contributions from industry and academia will help clarify where AI already delivers value, where expectations may exceed current capabilities, and what developments are needed to enable broader implementation.</p><h5><strong>Discussion Topics:&nbsp;</strong></h5><ul><li>Current applications of AI and machine learning in separation science&nbsp;</li><li>Use of AI in chromatographic method development and optimization&nbsp;</li><li>Data analysis, peak detection, and automated interpretation of complex datasets&nbsp;</li><li>AI-supported troubleshooting and predictive maintenance of analytical instruments&nbsp;</li><li>Integration of AI into regulated environments (e.g., pharmaceutical industry)&nbsp;</li><li>Data requirements, data quality, and standardization challenges&nbsp;</li><li>Comparison between academic developments and real-world industrial implementation&nbsp;</li><li>Barriers to adoption, including regulatory considerations, validation requirements, and transparency of models&nbsp;</li><li>Future outlook: digital laboratories, autonomous method development, and AI-assisted decision-making in analytical workflows&nbsp;</li></ul><h5><strong>Format:</strong></h5><p>Moderated panel discussion with short introductory remarks from invited speakers representing both academia and industry, followed by an interactive discussion with the audience.</p><h4><strong>Activity 5: Discussion Session: Sustainability in Separation Science</strong></h4><ul><li>Wednesday, 9 September 2026 | 16:30-18:00</li><li><strong>Place:</strong> Club A</li><li><strong>Moderators:</strong> Gertrud Morlock</li></ul><h5><strong>Concept:</strong></h5><p>Sustainability has become an increasingly important priority in analytical science, driving the development of greener methods, reduced solvent consumption, improved energy efficiency, and more responsible laboratory practices. While academic research frequently proposes innovative sustainable approaches, their implementation in industry is often influenced by additional considerations such as robustness, regulatory compliance, cost efficiency, scalability, and long-term reliability.</p><p>This discussion session aims to explore how sustainability principles are applied in real industrial environments and how collaboration between academia and industry can help translate promising research concepts into practical, validated solutions.</p><p>By bringing together perspectives from both sectors, the session will highlight opportunities to better align research priorities with industrial needs and identify realistic pathways toward more sustainable separation workflows.</p><h5><strong>Discussion Topics:&nbsp;</strong></h5><ul><li>Reduction of solvent consumption and the transition to more environmentally friendly mobile phases&nbsp;</li><li>Miniaturized and more efficient separation and extraction techniques&nbsp;</li><li>Energy consumption and efficiency of laboratory processes and analytical instrumentation&nbsp;</li><li>Lifecycle considerations for columns, consumables, and laboratory waste&nbsp;</li><li>Metrics and approaches for measuring and evaluating the “greenness” of analytical methods&nbsp;</li><li>Regulatory constraints and requirements that may limit the implementation of greener analytical workflows&nbsp;</li></ul><h5><strong>Format:</strong></h5><p>Moderated panel discussion with short introductory contributions from invited speakers representing industry, academia, and technology providers, followed by interactive discussion with the audience.</p><h5><strong>Panelists:</strong></h5><ul><li>Sébastien Rolle, Shimadzu&nbsp;</li><li>Ken Cook, Thermo Fisher Scientific&nbsp;</li><li>Andreas Otto, Agilent&nbsp;</li><li>Janusz Pawliszyn, University of Waterloo&nbsp;</li><li>Giorgia Purcaro, University of Liège&nbsp;</li></ul>

Industry program at ISC 2026

<p><strong>We are delighted to invite food scientists from academia and industry, representatives of (inter)national agencies, control authorities, governmental and commercial laboratories to attend </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/928"><strong>RAFA 2026</strong></a><strong> (12th Symposium on Recent Advances in Food Analysis), that takes place 3–6 November 2026 in Prague..</strong></p><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://rafa.easyevent.eu/tickets/"><strong>SUBMIT ABSTRACT</strong></a> <strong>FOR POSTER PRESENTATION by August 31, 2026</strong></p><p>Do not miss the opportunity to present your latest results at <strong>RAFA 2026</strong>.</p></blockquote><h5><strong>Next deadlines:</strong></h5><ul><li><strong>August 31, 2026</strong>: 2nd window – &nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/sponsors_exhibitors_media_partners_info.html"><strong>Application for participation as a Sponsor or Exhibitor</strong></a></li><li><strong>September 30, 2026:&nbsp;</strong><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/registration.html"><strong>Registration at the regular rate</strong></a></li><li><strong>Abstract submission</strong> – <strong>Last minute posters </strong>(<i>May not appear in the RAFA 2026 program</i>)</li></ul></li></ul><p>Since its first edition in 2003, RAFA has grown into a <strong>prestigious international event</strong>, typically welcoming <strong>up to 850 participants</strong> from more than <strong>60 countries</strong> worldwide, with the support of more than 50 sponsors, exhibitors, media and supporting partners.</p><p>RAFA 2026 will provide an overview of contemporary trends in the <strong>ANALYTICAL &amp; BIOANALYTICAL STRATEGIES IN FOOD QUALITY AND SAFETY CONTROL</strong> and discuss the <strong>CHALLENGES AND NOVEL APPROACHES IN FOOD AND NATURAL PRODUCTS ANALYSIS</strong>.</p><p>View the <a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/invited_speakers.html"><strong>list of distinguished speakers</strong></a> who have accepted our invitation to RAFA 2026 and will present current hot topics and future perspectives in food analysis!</p><blockquote><p>👉More details on the <a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/scientific_programme.html"><strong>RAFA 2026 preliminary program</strong></a> can be found HERE.</p><p>👉OUR <a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/about_sponsors_exhibitors_partners.html"><strong>SPONSORS, EXHIBITORS, MEDIA and SUPPORTING PARTNERS</strong></a></p></blockquote><p>You are kindly invited to join and contribute to our exciting scientific program and unforgettable social events!</p><p><strong>We look forward to meeting you again in Prague!</strong></p><ul><li>Prof. Jana Pulkrabova &amp; Dr. Stefan van Leeuwen, Symposium Chairs</li><li>Prof. Michele Suman, Symposium Co-chair</li><li>Prof. Jana Hajslova &amp; Prof. Michel Nielen, Symposium Honorary Chairs</li></ul>

RAFA 2026 - Deadline for Abstract Submission for poster presentation is coming

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Food

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RAFA provides an overview of contemporary trends in ANALYTICAL & BIOANALYTICAL STRATEGIES IN FOOD QUALITY AND SAFETY CONTROL and discusses CHALLENGES / NOVEL APPROACHES IN FOOD AND NATURAL PRODUCTS ANALYSIS.

Recent Advances in Food Analysis (RAFA)

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Thermo Fisher Scientific

The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

ECOM Separchrom PC01-HPLC Industrial Preparative Columns with Axial Compression

Separchrom PC01 ECOM industrial preparative columns correspond to the highest requirements of preparative liquid chromatography. They are designed to be used in a mode of axial compression with a moving input piston. 

<div style="display:block;" aria-labelledby="tab_item-0"><p><strong>HPLC Columns for prep chromatography. High performance columns with axial compression.</strong></p><p><strong>Separchrom PC01 ECOM</strong> <strong>columns</strong> correspond to the highest requirements of <strong>preparative liquid chromatography</strong>. They are designed to be used in a mode of axial compression with a moving input piston. In the original design, they are equipped with identical pistons on input and output (biaxial compression as an option). In the ECOM Separchrom PC FE modification, they have a fixed end plate on the output instead of the piston. Pistons and plates are equipped with frits. A liquid distributing system is installed under each frit. The design guarantees perfect piston flow, high efficiency even for high injection volume, and simple accessibility of frits for cleaning. Columns can be packed by dynamic slurry method or by sedimentation or with sorbent in a dry state.</p><p><strong>SEPARCHROM PC 01 ECOM columns</strong> are made of stainless steel AISI 316L (tube inner surface is polished to reach Ra &lt; 0.3 µm) with stainless steel pistons and UHMWPE (PTFE on the request) sealing rings. The stroke of the main piston is proportional to the used hydraulic cylinder or flange bolt length. Pistons are sectional; the frit (sintered mesh disc 3 µm pore size, 5 mm thickness) with the frit ring can be removed. The output plate in the FE version is made of UHMWPE, frit is fixed with a simple frit ring. Column flanges are not welded, but screwed onto the column tube. No temperature increase during manufacturing means no change in steel properties. The column stand made of stainless steel profiles is included for columns over 100 mm I.D.</p><p>Hydraulic cylinders fitted on the upper flange are offered with manual oil pumps as well as with fully automated electric motor-driven devices for column packing/unpacking procedures.</p><h3><strong>Accessories</strong></h3><p>Columns are offered with a broad scale of accessories such as filling adapters, slurry mixing vessels, slurry transporting pumps, etc.</p><h3><strong>COLUMN CONFIGURATION EXAMPLES</strong></h3><p>Columns are custom-made, do not hesitate to ask for any other sizes within the possible ranges</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_1_1795482301.jpg" alt="ECOM: Separchrom PC01-HPLC Column - available configuration - part 1" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_1_1795482301.jpg 236w," sizes="100vw" width="1440" height="952"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_2_6d3198af67.jpg" alt="ECOM: Separchrom PC01-HPLC Column - available configuration - part 2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_2_6d3198af67.jpg 239w," sizes="100vw" width="1442" height="942"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_3_9fd77a1054.jpg" alt="ECOM: Separchrom PC01-HPLC Column - available configuration - part 3" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_3_9fd77a1054.jpg 245w," sizes="100vw" width="1435" height="315"></p></div>

ECOM Separchrom PC02-MPLC (Medium-pressure) Columns for Prep Chromatography

Industrial-scale Preparative Columns PC02 - Columns for medium-pressure with axial bed compression for prep chromatography.

<div style="display:block;" aria-labelledby="tab_item-0"><p><strong>ECOM Separchrom PC02 medium-pressure stainless steel columns are intended for separation where high pressure is not necessary, but good efficiency can still be expected. They are made of stainless steel tubes.</strong></p><p>Part of the column is an <strong>input piston made of ultra-high molecular weight polyethylene</strong> and it is equipped with <strong>stainless steel support</strong> and <strong>stainless steel mesh frit</strong>. Flow distributor with groves is incorporated into the piston to guarantee piston flow with nearly zero pressure drop. The design guarantees simple accessibility of frits for cleaning.</p><p>Columns can be <strong>packed by dynamic slurry method</strong>, or <strong>by sedimentation</strong>, or <strong>with sorbent in the dry state</strong>, both with <strong>silica</strong> and <strong>polymer sorbents</strong>. For soft polymer sorbents, ECOM Separchrom PC02 oil systems can be equipped with a special software application to compensate for the swelling and unswelling of sorbents in different mobile phases (the column piston is moved in very small steps up and down during use).</p><h3><strong>Materials</strong></h3><p>Medium pressure prep chromatography column ECOM SEPARCHROM PC 02 is made of AISI 316L stainless steel, UHMWE moving pistons are equipped with integral sealing wings. Frits (stainless steel mesh 5 µm pore size, 5 mm thickness) can be fixed with a stainless steel ring. The stand made of stainless steel profiles is included.</p><h3><strong>COLUMN CONFIGURATION EXAMPLES</strong></h3><p>Columns are custom-made, do not hesitate to ask for any other sizes within the possible ranges</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_02_HPLC_Column_available_configuration_part_1_dfb61faf03.jpg" alt="ECOM: Separchrom PC02-HPLC Column - available configurations - part 1" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_02_HPLC_Column_available_configuration_part_1_dfb61faf03.jpg 245w," sizes="100vw" width="1641" height="939"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_02_HPLC_Column_available_configuration_part_2_cb9f807757.jpg" alt="ECOM: Separchrom PC02-HPLC Column - available configurations - part 2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_02_HPLC_Column_available_configuration_part_2_cb9f807757.jpg 245w," sizes="100vw" width="1645" height="364"></p></div>

ECOM Separchrom PC04-Non-metal Columns for Prep Chromatography

Industrial-scale preparative non-metal columns for prep chromatography with axial compression correspond to the highest requirements of preparative liquid chromatography. 

<div style="display:block;" aria-labelledby="tab_item-0"><p><strong>Polyethylene cartridge columns are usually intended for flash chromatography.</strong> We offer powerful<strong> ECOM Separchrom PC04 columns </strong>made of high-quality ultra-high molecular weight polyethylene, equipped with moving pistons and with no metal in contact with the mobile phase. Non-metal columns are required in biochromatography and some other special applications.</p><p>Plastic columns for preparative liquid chromatography <strong>ECOM SEPARCHROM PC 04</strong>, are unfilled, and made of ultra-high molecular weight polyethylene. They are equipped with the same pistons on the input and the output. The input piston is moved either by flange bolts (type A), or by a central stainless steel threaded rod (type B). The pistons are fitted with plastic polypropylene frits 10 µm and a distribution system.</p><h3><strong>COLUMN CONFIGURATION EXAMPLES</strong></h3><p>Columns are custom-made, do not hesitate to ask for any other sizes within the possible ranges</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_04_HPLC_Column_available_configurations_8767eb40ae.jpg" alt="ECOM: Separchrom PC04-HPLC Column - available configurations" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_04_HPLC_Column_available_configurations_8767eb40ae.jpg 245w," sizes="100vw" width="1645" height="823"></p></div>

ECOM ION-QN and ION-QD Chiral HPLC Columns

 Weak Anion-Exchanger (WAX) stationary phases for reliable chiral separation of organic acids and amino acids. The most efficient approach to purify and resolve even the most troublesome enantiomeric mixtures.

<p><strong>Weak Anion-Exchanger (WAX) stationary phases for reliable chiral separation of organic acids and amino acids.&nbsp;</strong></p><p>We introduce our own generic series of weak anion-exchangers, based on <strong>tert-butyl-carbamoylated Cinchona alkaloids quinine (QN)</strong> and <strong>quinidine (QD)</strong>. Our Chiral ION-QN and Chiral ION-QD columns contain a well-balanced pair of pseudoenantiomeric chiral selectors, designed to efficiently separate enantiomers of acidic compounds. These columns represent the most efficient approach to purify and resolve even the most troublesome enantiomeric mixtures.</p><h4><strong>Applications</strong></h4><ul><li>Chiral ION-QN and Chiral ION-QD are ideal for enantioseparations of chiral acidic compounds, including:</li><li>N-protected amino acids</li><li>Aminophosphonic &amp; Aminosulfonic acids</li><li>Lactic &amp; Thiolactic acids</li><li>Clenbuterol &amp; Thyroxine</li></ul><h4><strong>Key Benefits</strong></h4><ul><li>Provide better separation power and increased enantioselectivity (in comparison to other available alternatives)</li><li>Allow elegant switch of the elution order of enantiomers</li><li>Demonstrated high stability against all common HPLC solvents</li><li>Enable enhanced method development</li><li>Available at analytical and preparative scale</li></ul><h4><strong>Columns</strong></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Chiral_HPLC_Column_ION_QN_and_ION_QD_6_085a58fc5e.jpg" alt="ECOM: Chiral HPLC Column ION-QN and ION-QD" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Chiral_HPLC_Column_ION_QN_and_ION_QD_6_085a58fc5e.jpg 245w," sizes="100vw" width="1059" height="531"></p><h4><strong>Features&nbsp;</strong></h4><ul><li><strong>Max. pressure</strong>: 5800 psi (400 bar)</li><li><strong>Particle size</strong>: 3 or 5 μm</li><li><strong>pH range</strong>: 2 to 9</li><li><strong>Pore size</strong>: 120 Å</li><li><strong>Surface area</strong>: 300 m²/g</li><li><strong>Max. temperature</strong>: 60 °C</li><li><strong>Packing material</strong>: Spherical, Fully Porous</li><li><strong>Carbon load</strong>: 13.0 %</li><li><strong>End-capped</strong>: Yes</li><li><strong>Product line</strong>: ION</li></ul><p>The mobile phase composition (methanol-formic acid-ammonium formate) has been optimized for preparative applications to provide high column loadability, fast analyte elution, and good peak shape. Moreover, the buffer allows for simple product harvesting by solvent evaporation and subsequent ammonium formate sublimation 50 °C / 10 torr.</p><p>The anion exchangers are perfect for the purification of organic acids and their chiral separation. They are very efficient in the chiral resolution of amino acids, but also other derivatives, such as amino phosphonic acids. Aminophosphonic acids are important precursors in the synthesis of phosphopeptidomimetics, which may potentially serve as new-generation pharmaceuticals.</p>

Phenomenex Axia Preparative LC Columns

Available in over 40 unique achiral and chiral selectivities, Axia advanced preparative column packings and column hardware designs offer several advantages.

<p class="cke-user_style-important"><strong>Guaranteed, Longest Lasting Preparative HPLC and SFC Column</strong></p><p>Available in over 40 unique achiral and chiral selectivities, Axia advanced preparative column packings and column hardware designs offer several advantages. Unlike traditional preparative HPLC column packing methods, the Axia packing method offers increased sorbent bed density for increased performance and eliminates media bed collapse as a source of premature column failure in preparative HPLC/SFC columns.</p><h3><strong>Axia Packed Preparative HPLC Columns</strong></h3><h4><strong>Axia Single Axial Compression Step</strong></h4><p>Axia packed preparative columns involve a single axial compression step, unlike conventional packed preparative columns. The ideal prep HPLC column bed density is custom calculated and automated for each specific media and column size. Computer control of the entire process ensures both proper bed density and column uniformity every time.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Axia_Packing_Technology_1785ff0442.jpg" alt="Phenomenex: Axia Packing Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Axia_Packing_Technology_1785ff0442.jpg 245w," sizes="100vw" width="917" height="560"></p><h4><strong>Unmatched Column Reproducibility</strong></h4><p>The <strong>automated</strong> <strong>Axia™ packing system</strong> provides <strong>feedback control</strong> and <strong>infinite tuning</strong> of packing density for specific media characteristics such as mechanical strength and porosity. An optimum higher bed density can be consistently reproduced column-to-column. This directly translates into consistent efficiency and peak asymmetry measurements and decreases the column variability seen in traditionally packed preparative columns.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Unmatched_Column_Reproducibility_a752be8dd5.jpg" alt="Phenomenex: Unmatched Column Reproducibility" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Unmatched_Column_Reproducibility_a752be8dd5.jpg 245w," sizes="100vw" width="995" height="534"></p><h3><strong>Seamless Scalability to Increase Sample Load</strong></h3><h4><strong>Increase Column Length</strong></h4><p>Increase sample load without increasing your flow rate by using a longer column. With Axia technology, each preparative column is optimized for:</p><ul><li>Analytical-like efficiency</li><li>Long column lifetime</li><li>High sample load with high surface area media such as Kinetex, Aeris, Gemini, Luna, Luna Omega, or Synergi</li></ul><h4><strong>Increase Column ID</strong></h4><p>For maximizing load without increasing the run time, consider scaling up to a larger column ID. Axia packed columns provide the three important benefits you need.</p><ul><li>Reproducible performance across all column diameters</li><li>Increased throughput without sacrificing purity</li><li>High efficiency from analytical to preparative</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Increase_Column_ID_a45d7f8aa3.jpg" alt="Phenomenex: Increase Column ID" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Increase_Column_ID_a45d7f8aa3.jpg 156w," sizes="100vw" width="908" height="910"></p><h3><strong>Axia Technology Outperforms Traditional Packing Processes!</strong></h3><p>Because of the constant pressure placed on the integrated packing piston, Axia packed columns possess the dynamic capability of maintaining a consistent, homogeneous media bed. This results in superior column performance no matter which media selectivity you choose.</p><h3><strong>How to choose the right column</strong></h3><h5><strong>Acidic Compounds</strong></h5><ul><li>Kinetex XB-C18</li><li>Gemini C18</li></ul><h5><strong>Aromatic</strong></h5><ul><li>Luna Phenyl-Hexyl</li><li>Kinetex Biphenyl</li></ul><h5><strong>Closely Related Compounds</strong></h5><ul><li>Kinetex F5</li><li>Luna PFP(2)</li></ul><h5><strong>Extremely Hydrophobic</strong></h5><ul><li>Kinetex C8</li><li>Luna C8(2)</li></ul><h5><strong>Extremely Polar</strong></h5><ul><li>Luna HILIC(2)</li><li>Kinetex HILIC</li></ul><h5><strong>Hydrophobic Compounds</strong></h5><ul><li>Luna C18(2)</li><li>Kinetex C18</li></ul><h5><strong>Polar Acids</strong></h5><ul><li>Luna Omega Polar C18</li><li>Kinetex Polar C18</li></ul><h5><strong>Polar Bases</strong></h5><ul><li>Luna Omega PS C18</li><li>Kinetex PS C18</li></ul><h5><strong>Sugars</strong></h5><ul><li>Luna NH(2)</li></ul><h5><strong>Chiral</strong></h5><ul><li>Lux i-Amylose-3</li><li>Lux Cellulose-1</li></ul>

Products from category HPLC columns focused on PrepLC - page 1

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