This workflow confirms and quantifies 31 drugs of abuse in oral fluid (including THC) in a 4.5‑minute LC run on the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149">Thermo Scientific Stellar mass spectrometer</a> using full scan + targeted MS2 (tMS2) with both HCD and CID fragmentation. With 1 µL injection and 0.1% formic acid in water/methanol mobile phases, the method supports ~13 samples/hour (up to ~320/day continuous operation) while maintaining confirmation performance via ion ratios.<blockquote><h5>Method at a glance</h5><ul><li>What matrix is tested? Oral fluid</li><li>How many drugs? 31 drugs of abuse (panel includes THC)</li><li>Run time? 4.5 minutes</li><li>Throughput? ~13 samples/hour → up to ~320 samples/day (24‑hour operation)</li><li>Injection volume? 1 µL</li><li>Mobile phases? 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B)</li><li>MS acquisition? Full scan + targeted MS2 (tMS2)</li><li>Fragmentation? Compound‑optimized HCD and CID in the same targeted list</li><li>Confirmation strategy? Ion ratios with ±20% tolerance (typical confirmatory approach)</li></ul></blockquote><h3>Why oral fluid—and why speed matters</h3>Oral fluid is attractive for clinical and forensic toxicology because collection is simple and the matrix aligns well with high‑volume workflows (workplace/occupational, roadside, and other settings). The challenge is building a method that’s simultaneously:<ul><li>Broad enough for today’s drug panels</li><li>Sensitive enough to reach required cutoffs</li><li>Fast enough to keep sample queues under control</li><li>Confirmation‑ready (consistent product ions and ion ratios)</li></ul>A 4.5‑minute method is a practical lever here: compress runtime, raise throughput, and still keep confirmation quality by optimizing MS/MS conditions per compound.<h3>The LC method</h3>A short, efficient separation is the foundation of the workflow:<ul><li>UHPLC system: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Thermo Scientific Vanquish Horizon UHPLC</a></li><li>Column: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/424">Thermo Scientific Accucore Biphenyl</a>, 50 × 2.1 mm, 2.6 µm</li><li>Mobile phase A: 0.1% formic acid in water</li><li>Mobile phase B: 0.1% formic acid in methanol</li><li>Injection volume: 1 µL</li><li>Gradient runtime: 4.5 minutes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_fd3f3e8ea4.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 1: Thermo Scientific Vanquish Horizon UHPLC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_fd3f3e8ea4.jpg 120w," sizes="100vw" width="747" height="969"><h3>The MS method (Full scan + targeted MS2 with HCD + CID)</h3><h5>Acquisition approach</h5>This method uses a “two‑layer” strategy for quantitation + confirmation:<ol><li>Full scan (broad coverage, context, quantitative support)</li><li>Targeted MS2 (tMS2) (confirmation‑focused product ions, ion ratios)</li></ol><h5>The key differentiator: HCD and CID in one targeted list</h5>Instead of forcing every analyte into a single fragmentation style, the method uses both:<ul><li>HCD (beam‑type fragmentation)</li><li>CID (resonance‑type fragmentation)</li></ul><h5>Why that improves method development:</h5>Drugs of abuse span multiple chemistries. A single collision approach often works “okay” for most compounds—but one or two analytes can become persistent problems (weak qualifiers, unstable ion ratios, excess noise). Having both HCD and CID available in one method lets you optimize per compound and move faster.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_3_e4a95651a2.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 2: Thermo Scientific Stellar mass spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_3_e4a95651a2.jpg 245w," sizes="100vw" width="645" height="377"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_5_88276eec6a.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 3: Full scan &amp; targeted MS2 methods, HCD and collision energies used." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_5_88276eec6a.jpg 245w," sizes="100vw" width="768" height="279"><h3>Why CID + HCD flexibility improves confirmation</h3>In this workflow, HCD performed well for most compounds. But for buprenorphine, HCD produced one dominant product ion with additional low m/z noise and noticeable residual precursor. Using CID generated two high‑intensity product ions and delivered consistent ion ratios across the calibration range.When your confirmation hinges on stable ion ratios, the ability to switch fragmentation mode (not just collision energy) is a method‑development advantage—especially for “problem analytes.”<h3>Controllable ionization</h3>Oral fluid methods often deal with limited sample volume and matrix effects. This workflow pairs low injection volume with ionization control using the Thermo Scientific OptaMax Plus HESI source.<ul><li>The OptaMax Plus HESI source supports high probe temperature capability (up to 650 °C).</li><li>In this study, a vaporizer temperature of 500 °C was used to help achieve sensitivity and improve relative peak areas across drugs of abuse.</li></ul>Why this matters: If you’re injecting only 1 µL, ionization stability and efficiency can be the difference between “meets cutoff” and “misses at LOQ.”<h3>Sample preparation</h3>Oral fluid panels become more challenging when THC is included because most drugs of abuse are basic, while THC is largely neutral, and that can stress extraction chemistry.In this workflow, oral fluid calibrators and samples were extracted using a mixed‑mode approach designed to recover THC alongside other drugs:<ul><li>Calibrators prepared across eight levels (broad dynamic range)</li><li>Extraction performed using mixed‑mode chemistry tips (SCX/WAX) in a dispersive SPE‑style workflow</li><li>Steps automated on a liquid handling platform to support high‑throughput consistency</li><li>Samples dried and reconstituted prior to LC‑MS analysis</li><li>Only 1 µL injected per run</li></ul>Even if your lab uses different automation or consumables, the method structure (fast LC + full scan + targeted MS2 with dual fragmentation) transfers well.<blockquote><h5>Throughput math</h5><ul><li>4.5 minutes per injection → 60 / 4.5 = 13.3 injections/hour</li><li>That’s ~13 samples/hour in typical phrasing</li><li>Over 24 hours: 13.3 × 24 = ~320 samples/day (continuous operation)</li></ul>If you run a single shift, just scale accordingly (e.g., ~106/day for an 8‑hour shift, excluding QC and overhead).</blockquote><h3>Performance summary</h3><h5>Sensitivity and linearity</h5><ul><li>Linearity from LOQs as low as 0.25 ng/mL up to 1,000 ng/mL (panel-dependent)</li><li>For THC specifically: LOQ at 1 ng/mL, with a dynamic range extending to 1,000 ng/mL in this evaluation</li><li>Across the panel, LOQs were reported as lower than recommended confirmation cutoffs referenced in SAMHSA guidance and NSC Tier 1 cutoffs (study-specific outcome)</li></ul><h5>Confirmation via ion ratios</h5><ul><li>Ion ratios evaluated using ±20% tolerance (typical confirmatory acceptance framework)</li><li>The workflow produced consistent ion ratios across calibrator levels for the full panel in this evaluation</li></ul><h5>Case sample comparison (real-world relevance)</h5>When compared against a validated triple quadrupole method for case study samples, the Stellar MS workflow:<ul><li>Confirmed the positive hits observed by the reference method</li><li>Reported a few additional positives for select analytes, attributed to lower LOQs</li><li>Highlighted an operational efficiency point: 1 µL injection in this method versus 20 µL injection in the compared method</li></ul><h3>FAQ</h3><div><div><ol><li>How long does the LC‑MS run take for the 31‑drug oral fluid panel? 4.5 minutes per injection using a short UHPLC gradient. &nbsp;</li><li>How many drugs of abuse can Stellar MS detect in this workflow? 31 drugs of abuse, including THC, using full scan + targeted MS2. &nbsp;</li><li>What MS/MS approach is used? A combined strategy: full scan plus targeted MS2 (tMS2) for confirmation. &nbsp;</li><li>Why use both HCD and CID? Because some analytes fragment better—and produce more stable confirmation ions/ion ratios—under one mode versus the other. Using both increases flexibility and speeds method optimization. &nbsp;</li><li>What injection volume is used? 1 µL of extracted sample. &nbsp;</li><li>What mobile phases are used? 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B). &nbsp;</li><li>How many samples can you run per day? At 4.5 minutes/injection, throughput is ~13 samples/hour or up to ~320/day with continuous operation (actual throughput depends on QC and scheduling).</li></ol></div></div><h3>What makes this workflow “easy” to develop on Stellar MS</h3>Here’s the short list of features that directly reduce method‑development friction:<ul><li>HCD or CID in one method → optimize per compound, not compromise</li><li>Fast MS2 acquisition speeds (up to 140 Hz for MS2) → more data points across narrow peaks</li><li>Fast polarity switching (true 5 ms) → supports mixed panels efficiently</li><li>Adaptive RT (real‑time retention time window adjustment) → helps reduce reinjections when retention times drift (e.g., column aging) and supports method transfer from longer to shorter gradients</li><li>OptaMax Plus HESI source control → supports sensitivity at low injection volumes</li></ul>If you’re building a fast oral fluid confirmation workflow—or updating an existing DOA panel—this 4.5‑minute method shows how Stellar MS can combine speed, confirmation-ready MS2, and fragmentation flexibility (HCD + CID) in a single targeted workflow. Reach out to learn how to adapt the targeted list, collision settings, and scheduling strategy to your lab’s cutoffs and throughput goals.<blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#MassSpectrometry #OralFluid #DrugsOfAbuse #MethodDevelopment #HighThroughput</blockquote>

4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS

<h2><a target="_blank" rel="noopener noreferrer" href="https://www.isc2026.org/">ISC 2026</a></h2>The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/314">International Symposium on Chromatography (ISC)</a> represents the oldest conference series on separation science. ISC symposia have been organised since 1956 in each even year. ISC is one of the premier meetings series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/644">35th International Symposium on Chromatography</a></h4>📆6. - 10. September 2026📍Prague, Czech Republic<h3>REGISTRATION</h3><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://www.czech-in.org/cmPortalV15/Account/Login?ReturnUrl=%2FcmPortalV15%2FPortal%2FISC26%2Fnormal">Register HERE for the ISC 2026</a>Late Registration until 25 August 2026</blockquote><h3>INDUSTRY ACTIVITIES</h3><h4>Activity 1: Discussion Session: Industry &amp; Education</h4><ul><li>Monday, 7 September 2026 | 16:30-17:50</li><li>Place: Club A</li><li>Moderators: Tony Edge and Deirdre Cabooter&nbsp;</li></ul><h5>Concept:</h5>This discussion session will explore the relationship between education in separation science in academia and the expectations of industry, focusing on how universities and companies can better collaborate to prepare graduates for successful careers in analytical laboratories and R&amp;D environments. The goal of the session is to provide perspectives from both academia and industry on how education in chromatography and related separation techniques can evolve to better reflect current technological, methodological, and regulatory challenges.<h5>Discussion Topics:</h5><ul><li>Bridging academia and industry: how both sectors perceive current cooperation and where improvements are possible&nbsp;</li><li>Graduate preparedness: which skills and competencies are often missing when entering industrial roles, and in which areas graduates typically excel&nbsp;</li><li>Approaches to teaching separation science: how chromatography and related analytical techniques are taught across different universities and regions&nbsp;</li><li>Industry support for education: examples of collaboration such as internships, industrial placements, joint teaching activities, or access to instrumentation&nbsp;</li><li>Experiences with academia–industry partnerships: what models of cooperation have proven effective in practice&nbsp;</li><li>Future perspectives: how education in separation science should adapt to emerging trends such as automation, digitalization, sustainability, and regulatory requirements&nbsp;</li></ul><h5>Format:</h5>Moderated panel discussion with short introductory remarks from invited speakers representing both academia and industry, followed by an interactive discussion with the audience.<h5>Panelists:</h5><ul><li>Shimadzu&nbsp;</li><li>Novartis&nbsp;</li><li>Jelle De Vos, RIC Group&nbsp;</li><li>Davy Guillarme, University of Geneva&nbsp;</li><li>Caterina Temporini, University of Pavia&nbsp;</li></ul><h4>Activity 2: Career Presentation&nbsp;</h4><ul><li>Monday, 7 September 2026 | 18:00-19:00</li><li>Place: Club A</li><li>Moderators: Adrian Clarke and Martina Catani</li></ul><h5>Concept:</h5>This session will provide an overview of career opportunities in separation science across industry, highlighting the wide range of professional pathways available to graduates and experienced scientists working in chromatography and related analytical disciplines.Participating companies will introduce their organizations, present typical roles involving separation techniques, and share insights into the skills and competencies they seek in candidates. The session is intended to support students, early-career researchers, and experienced professionals interested in exploring career options in industry sectors.<h5>Discussion Topics:&nbsp;</h5><ul><li>Overview of career paths involving chromatography and separation science&nbsp;</li><li>Entry-level opportunities for graduates and PhD holders&nbsp;</li><li>Positions for experienced scientists and technical specialists&nbsp;</li><li>Roles in R&amp;D, analytical development, quality control, regulatory science, application support, and instrument development&nbsp;</li><li>Skills and competencies valued by employers in separation science&nbsp;</li><li>Internships, graduate programs, and early-career development opportunities&nbsp;</li><li>Mobility between academia and industry&nbsp;</li></ul><h5>Format:</h5>Short presentation providing:<ul><li>overview of different carrier paths&nbsp;</li><li>introduction to the company and its activities related to separation science&nbsp;</li><li>examples of typical roles and career progression paths&nbsp;</li><li>information about current or expected hiring needs&nbsp;</li><li>advice for candidates interested in pursuing careers in industry&nbsp;</li><li>the session should include time for informal discussion, allowing participants to interact with company representatives and learn more about specific opportunities.&nbsp;</li></ul><h4>Activity 3: Job Fair</h4><ul><li>Monday, 7 September and Tuesday, 8 September 2026 | 12:30-14:00</li><li>Place: Club A</li><li>Main organizer: Adrian Clarke</li></ul><h5>Concept:</h5>The ISC 2026 Job Fair provides a structured opportunity for direct interaction between companies active in separation science and conference participants seeking career opportunities. The session is designed to facilitate efficient, focused conversations between employers and candidates interested in positions related to chromatography and analytical science.Participating candidates will submit their CV in advance allowing companies to review profiles prior to the event and identify potential matches based on expertise, experience, and career interests.<h5>Format:</h5>The Job Fair will follow a structured rotation format:<ul><li>Each participating company will be assigned a dedicated table&nbsp;</li><li>One company representative will meet candidates individually&nbsp;</li><li>Each candidate will have 5 minutes per meeting to introduce their background and interests&nbsp;</li><li>Participants will rotate between companies at timed intervals&nbsp;</li><li>Session timing will be managed by the organizer, with a signal indicating when to move to the next meeting&nbsp;</li></ul><h5>Candidates:</h5>Participants interested in joining the Job Fair are asked to:<ul><li>submit their CV before August 23rd &nbsp;</li><li>indicate areas of expertise and career interests&nbsp;</li><li>specify whether they are seeking internships, entry-level roles, or experienced positions&nbsp;</li><li>specify if you cannot be available on a certain day e.g. owing to poster presentation&nbsp;</li><li>send these materials to: <a href="mailto:careers@isc2026.org">careers@isc2026.org</a>&nbsp;</li></ul><h5>Companies:</h5>Confirmed participating companies to date include:<ul><li>Novartis&nbsp;</li><li>RIC Group&nbsp;</li><li>Shimadzu&nbsp;</li><li>further companies to be added&nbsp;</li></ul>Potential employers please register your interest to <a href="mailto:careers@isc2026.org">careers@isc2026.org</a> to participate in the speed-dating (first come first served basis).“Jobs position” board will be available in the Careers fair in the foyer in the symposium venue and attendees can visit the company booths in the exhibition hall or discuss with recruiting companies during the conference.<blockquote>Recruiters please send an email with your open positions to <a href="careers@isc2026.org">careers@isc2026.org</a> by the 23rd August and bring the job adverts to post on the jobs board (there will also be the possibility to upload the open positions to the Symposium app).</blockquote><h4>Activity 4: Discussion Session: Artificial Intelligence in Separation Science: From Concept to Practice</h4><ul><li>Tuesday, 8 September 2026 | 16:30-18:00</li><li>Place: Club A</li><li>Moderators: Bob Pirok</li></ul><h5>Concept:</h5>Artificial intelligence (AI) and data-driven approaches are increasingly influencing analytical chemistry, offering new possibilities for method development, data interpretation, process optimization, and decision support. This discussion session will explore how AI is currently being applied in separation science, with a particular focus on practical implementation in industry environments.The session aims to provide a realistic overview of the current state of AI adoption, highlighting both successful applications and remaining challenges. Contributions from industry and academia will help clarify where AI already delivers value, where expectations may exceed current capabilities, and what developments are needed to enable broader implementation.<h5>Discussion Topics:&nbsp;</h5><ul><li>Current applications of AI and machine learning in separation science&nbsp;</li><li>Use of AI in chromatographic method development and optimization&nbsp;</li><li>Data analysis, peak detection, and automated interpretation of complex datasets&nbsp;</li><li>AI-supported troubleshooting and predictive maintenance of analytical instruments&nbsp;</li><li>Integration of AI into regulated environments (e.g., pharmaceutical industry)&nbsp;</li><li>Data requirements, data quality, and standardization challenges&nbsp;</li><li>Comparison between academic developments and real-world industrial implementation&nbsp;</li><li>Barriers to adoption, including regulatory considerations, validation requirements, and transparency of models&nbsp;</li><li>Future outlook: digital laboratories, autonomous method development, and AI-assisted decision-making in analytical workflows&nbsp;</li></ul><h5>Format:</h5>Moderated panel discussion with short introductory remarks from invited speakers representing both academia and industry, followed by an interactive discussion with the audience.<h4>Activity 5: Discussion Session: Sustainability in Separation Science</h4><ul><li>Wednesday, 9 September 2026 | 16:30-18:00</li><li>Place: Club A</li><li>Moderators: Gertrud Morlock</li></ul><h5>Concept:</h5>Sustainability has become an increasingly important priority in analytical science, driving the development of greener methods, reduced solvent consumption, improved energy efficiency, and more responsible laboratory practices. While academic research frequently proposes innovative sustainable approaches, their implementation in industry is often influenced by additional considerations such as robustness, regulatory compliance, cost efficiency, scalability, and long-term reliability.This discussion session aims to explore how sustainability principles are applied in real industrial environments and how collaboration between academia and industry can help translate promising research concepts into practical, validated solutions.By bringing together perspectives from both sectors, the session will highlight opportunities to better align research priorities with industrial needs and identify realistic pathways toward more sustainable separation workflows.<h5>Discussion Topics:&nbsp;</h5><ul><li>Reduction of solvent consumption and the transition to more environmentally friendly mobile phases&nbsp;</li><li>Miniaturized and more efficient separation and extraction techniques&nbsp;</li><li>Energy consumption and efficiency of laboratory processes and analytical instrumentation&nbsp;</li><li>Lifecycle considerations for columns, consumables, and laboratory waste&nbsp;</li><li>Metrics and approaches for measuring and evaluating the “greenness” of analytical methods&nbsp;</li><li>Regulatory constraints and requirements that may limit the implementation of greener analytical workflows&nbsp;</li></ul><h5>Format:</h5>Moderated panel discussion with short introductory contributions from invited speakers representing industry, academia, and technology providers, followed by interactive discussion with the audience.<h5>Panelists:</h5><ul><li>Sébastien Rolle, Shimadzu&nbsp;</li><li>Ken Cook, Thermo Fisher Scientific&nbsp;</li><li>Andreas Otto, Agilent&nbsp;</li><li>Janusz Pawliszyn, University of Waterloo&nbsp;</li><li>Giorgia Purcaro, University of Liège&nbsp;</li></ul>

Industry program at ISC 2026

We are delighted to invite food scientists from academia and industry, representatives of (inter)national agencies, control authorities, governmental and commercial laboratories to attend <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/928">RAFA 2026</a> (12th Symposium on Recent Advances in Food Analysis), that takes place 3–6 November 2026 in Prague..<blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://rafa.easyevent.eu/tickets/">SUBMIT ABSTRACT</a> FOR POSTER PRESENTATION by August 31, 2026Do not miss the opportunity to present your latest results at RAFA 2026.</blockquote><h5>Next deadlines:</h5><ul><li>August 31, 2026: 2nd window – &nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/sponsors_exhibitors_media_partners_info.html">Application for participation as a Sponsor or Exhibitor</a></li><li>September 30, 2026:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/registration.html">Registration at the regular rate</a></li><li>Abstract submission – Last minute posters (May not appear in the RAFA 2026 program)</li></ul></li></ul>Since its first edition in 2003, RAFA has grown into a prestigious international event, typically welcoming up to 850 participants from more than 60 countries worldwide, with the support of more than 50 sponsors, exhibitors, media and supporting partners.RAFA 2026 will provide an overview of contemporary trends in the ANALYTICAL &amp; BIOANALYTICAL STRATEGIES IN FOOD QUALITY AND SAFETY CONTROL and discuss the CHALLENGES AND NOVEL APPROACHES IN FOOD AND NATURAL PRODUCTS ANALYSIS.View the <a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/invited_speakers.html">list of distinguished speakers</a> who have accepted our invitation to RAFA 2026 and will present current hot topics and future perspectives in food analysis!<blockquote>👉More details on the <a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/scientific_programme.html">RAFA 2026 preliminary program</a> can be found HERE.👉OUR <a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/about_sponsors_exhibitors_partners.html">SPONSORS, EXHIBITORS, MEDIA and SUPPORTING PARTNERS</a></blockquote>You are kindly invited to join and contribute to our exciting scientific program and unforgettable social events!We look forward to meeting you again in Prague!<ul><li>Prof. Jana Pulkrabova &amp; Dr. Stefan van Leeuwen, Symposium Chairs</li><li>Prof. Michele Suman, Symposium Co-chair</li><li>Prof. Jana Hajslova &amp; Prof. Michel Nielen, Symposium Honorary Chairs</li></ul>

RAFA 2026 - Deadline for Abstract Submission for poster presentation is coming

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RAFA provides an overview of contemporary trends in ANALYTICAL & BIOANALYTICAL STRATEGIES IN FOOD QUALITY AND SAFETY CONTROL and discusses CHALLENGES / NOVEL APPROACHES IN FOOD AND NATURAL PRODUCTS ANALYSIS.

Recent Advances in Food Analysis (RAFA)

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Thermo Fisher Scientific

The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

Watrex P280 HPLC Pump Prep, stainless steel

Explore the future of preparative chromatography with PrecisionPrep™ P280, a state-of-the-art pump that redefines efficiency and precision.

Explore the future of preparative chromatography with PrecisionPrep™ P280, a state-of-the-art pump that redefines efficiency and precision.<blockquote>Price: 4 985<bdi>&nbsp;€ ex VAT</bdi></blockquote>Featuring a unique parallel double-piston arrangement, this preparative chromatography pump is designed to meet the demands of high-flow applications while ensuring a stable and precise flow profile.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_P280_HPLC_Pump_Prep_stainless_steel_b864d583c2.jpg" alt="Watrex P280 HPLC Pump Prep, stainless steel.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_P280_HPLC_Pump_Prep_stainless_steel_b864d583c2.jpg 235w," sizes="100vw" width="1024" height="681"><h3>PUMP FEATURES</h3><h5>Parallel Double-Piston Arrangement:</h5><ul><li>The innovative dual-piston design allows for parallel operation, optimizing flow distribution and enhancing efficiency.</li><li>Experience enhanced performance and productivity in preparative chromatography with this cutting-edge arrangement.</li><li>Achieve unmatched versatility with flow rates reaching up to 80 ml/min.</li><li>Tailor the pump’s performance to accommodate a wide range of preparative chromatography applications.</li></ul><h5>Stable and Precise Flow Profile:</h5><ul><li>Enjoy a stable and precise flow profile, critical for reproducibility in preparative separations.</li><li>The P280 ensures consistent and reliable results in every run, minimizing experimental variability.</li></ul><h5>User-Friendly Interface:</h5><ul><li>The user-friendly interface simplifies operation, allowing for seamless control and monitoring of preparative chromatography processes.</li><li>Easily program and adjust parameters to suit the specific requirements of your purification protocols.</li></ul><h5>Applications:</h5><ul><li>Purification of Pharmaceuticals</li><li>Isolation of Natural Products</li><li>Large-scale Biomolecule Purification</li><li>Industrial Separations/li&gt;</li></ul><h5>Reliability Beyond Measure:</h5><ul><li>PrecisionPrep™ P280 is engineered for durability and reliability in demanding preparative chromatography applications.</li><li>Trust in its robust construction and precise engineering to deliver consistent results run after run.</li></ul><h4>Conclusion</h4>Elevate your preparative chromatography to new heights with PrecisionPrep™ P280. With a parallel double-piston arrangement, high flow rates, and an efficient pulse dampener, this pump is designed to meet the challenges of large-scale separations while ensuring a stable and precise flow profile. Choose PrecisionPrep™ for unparalleled efficiency and reliability in preparative chromatography.<h3>TECHNICAL SPECIFICATION</h3><ul><li>Flow Rates: 0,1 ml/min – 80 ml/min with step 0.1 ml/min</li><li>Pressure: 5 MPa/725 psi</li><li>Flow precision: ± 0,2% RSD @ 10mL/min</li><li>Flow accuracy: ± 1% @ 10mL/min</li><li>Pressure accuracy: ± 2% of full-scale, with 80:20 H2O/IPA</li><li>Pulsation: better than ± 1% @ 10mL/min</li><li>Remote control: USB</li><li>I/O connector: Start, Stop, Status, Error</li></ul>You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/pumps/preparative-pumps/p280-pump-prep-stainless-steel/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Watrex P280 HPLC Pump Prep, PEEK, biocompatible

Explore the future of preparative chromatography with PrecisionPrep™ P280, a state-of-the-art pump that redefines efficiency and precision.<blockquote>Price: 4 985<bdi>&nbsp;€ ex VAT</bdi></blockquote>Featuring a unique parallel double-piston arrangement, this preparative chromatography pump is designed to meet the demands of high-flow applications while ensuring a stable and precise flow profile.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_P102_HPLC_pump_PEEK_5321aceaa0.jpg" alt="Watrex P280 HPLC Pump Prep, PEEK, biocompatible" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_P102_HPLC_pump_PEEK_5321aceaa0.jpg 208w," sizes="100vw" width="1024" height="768"><h3>PUMP FEATURES</h3><h5>Parallel Double-Piston Arrangement:</h5><ul><li>The innovative dual-piston design allows for parallel operation, optimizing flow distribution and enhancing efficiency.</li><li>Experience enhanced performance and productivity in preparative chromatography with this cutting-edge arrangement.</li><li>Achieve unmatched versatility with flow rates reaching up to 80 ml/min.</li><li>Tailor the pump’s performance to accommodate a wide range of preparative chromatography applications.</li></ul><h5>Stable and Precise Flow Profile:</h5><ul><li>Enjoy a stable and precise flow profile, critical for reproducibility in preparative separations.</li><li>The P280 ensures consistent and reliable results in every run, minimizing experimental variability.</li></ul><h5>User-Friendly Interface:</h5><ul><li>The user-friendly interface simplifies operation, allowing for seamless control and monitoring of preparative chromatography processes.</li><li>Easily program and adjust parameters to suit the specific requirements of your purification protocols.</li></ul><h5>Applications:</h5><ul><li>Purification of Pharmaceuticals</li><li>Isolation of Natural Products</li><li>Large-scale Biomolecule Purification</li><li>Industrial Separations/li&gt;</li></ul><h5>Reliability Beyond Measure:</h5><ul><li>PrecisionPrep™ P280 is engineered for durability and reliability in demanding preparative chromatography applications.</li><li>Trust in its robust construction and precise engineering to deliver consistent results run after run.</li></ul><h4>Conclusion</h4>Elevate your preparative chromatography to new heights with PrecisionPrep™ P280. With a parallel double-piston arrangement, high flow rates, and an efficient pulse dampener, this pump is designed to meet the challenges of large-scale separations while ensuring a stable and precise flow profile. Choose PrecisionPrep™ for unparalleled efficiency and reliability in preparative chromatography.<h3>TECHNICAL SPECIFICATION</h3><ul><li>Flow Rates: 0,1 ml/min – 80 ml/min with step 0.1 ml/min</li><li>Pressure: 5 MPa/725 psi</li><li>Flow precision: ± 0,2% RSD @ 10mL/min</li><li>Flow accuracy: ± 1% @ 10mL/min</li><li>Pressure accuracy: ± 2% of full-scale, with 80:20 H2O/IPA</li><li>Pulsation: better than ± 1% @ 10mL/min</li><li>Remote control: USB</li><li>I/O connector: Start, Stop, Status, Error</li></ul>You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/pumps/preparative-pumps/p280-pump-prep-peek-biocompatible/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Watrex P224 HPLC Pump, dual head, PEEK, biocompatible

WATREX P224 semiprep chromatography pump is a versatile and reliable tool commonly employed in laboratory settings, particularly in applications requiring precise dosing.

WATREX P224 semiprep chromatography pump is a versatile and reliable tool commonly employed in laboratory settings, particularly in applications requiring precise dosing.<blockquote>Price: 4 985<bdi>&nbsp;€ ex VAT</bdi></blockquote>This pump is designed for semipreparative chromatography, which involves the separation and purification of compounds at a larger scale than analytical chromatography but smaller than preparative chromatography. The stainless steel version of the pump offers durability and chemical resistance, making it suitable for use with a wide range of solvents and samples.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_P102_HPLC_pump_PEEK_5321aceaa0.jpg" alt="Watrex: P224 HPLC Pump, dual head, PEEK, biocompatible" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_P102_HPLC_pump_PEEK_5321aceaa0.jpg 208w," sizes="100vw" width="1024" height="768"><h3>PUMP FEATURES</h3><ul><li>Flow Rate Control: P224 allows for precise control of flow rates, crucial for achieving accurate and reproducible results in chromatographic separations.</li><li>Pressure Control: P224 has a built-in pressure control system to maintain consistent pressure throughout the chromatographic process, ensuring optimal column performance.</li><li>Gradient Capability: Two or up to four P224 pumps offer gradient capabilities, allowing for the creation of complex elution profiles controlled from PC to enhance separation efficiency.</li><li>User-Friendly Interface: An intuitive and user-friendly interface facilitates easy operating and monitoring of the pump parameters, contributing to efficient workflow in the laboratory.</li><li>Compatibility: P224 is compatible with a variety of chromatography instruments and stationary phases commonly used in semipreparative applications.</li><li>Reliability and Durability: A robust construction and high-quality materials ensure the pump’s durability and reliability, minimizing downtime and maintenance requirements.</li><li>Precision in Dosing: P224 design prioritizes accuracy in dosing, making it suitable for applications where precise control over sample injection and elution volumes is essential.</li><li>Safety Features: Incorporation of safety features such as pressure sensors, and overpressure protection ensures the well-being of both the operator and the equipment.</li><li>Maintenance and Calibration: P224 pumps are easy to maintain and calibrate, with clear documentation provided by us for routine upkeep and troubleshooting.</li><li>Versatility: P224 supports a wide range of solvents and mobile phases commonly used in chromatography, offering flexibility for various applications.</li><li>Data Logging and Connectivity: The capability to log service data enhances operation traceability and documentation.</li></ul><h3>TECHNICAL SPECIFICATION</h3><ul><li>Flow Rates: 0,1 ml/min – 25,00 ml/min with step 0.1 ml/min (semiprep)</li><li>Pressure: 30 MPa/4300 psi</li><li>Flow precision: &nbsp;&nbsp;&nbsp;&nbsp;± 0,2% RSD @ 1mL/min. and 7 Mpa</li><li>Flow accuracy: ± 1% @ 1mL/min. and 7 Mpa</li><li>Pressure accuracy: ± 2% of full-scale, with 80:20 H2O/IPA @ 7 Mpa</li><li>Pulsation: better than ± 0.5% @ 1mL/min. and 7 MPa</li><li>Remote control: USB</li><li>I/O connector: Start, Stop, Status, Error</li></ul>You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/pumps/preparative-pumps/p224-pump-dual-head-peek-biocompatible/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Watrex P224 HPLC Pump, dual head, Stainless Steel

WATREX P224 semiprep chromatography pump is a versatile and reliable tool commonly employed in laboratory settings, particularly in applications requiring precise dosing.<blockquote>Price: 4 985<bdi>&nbsp;€ ex VAT</bdi></blockquote>This pump is designed for semipreparative chromatography, which involves the separation and purification of compounds at a larger scale than analytical chromatography but smaller than preparative chromatography. The stainless steel version of the pump offers durability and chemical resistance, making it suitable for use with a wide range of solvents and samples.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_P280_HPLC_Pump_Prep_stainless_steel_b864d583c2.jpg" alt="Watrex: P224 HPLC Pump, dual head, Stainless Steel" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_P280_HPLC_Pump_Prep_stainless_steel_b864d583c2.jpg 235w," sizes="100vw" width="1024" height="681"><h3>PUMP FEATURES</h3><ul><li>Flow Rate Control: P224 allows for precise control of flow rates, crucial for achieving accurate and reproducible results in chromatographic separations.</li><li>Pressure Control: P224 has a built-in pressure control system to maintain consistent pressure throughout the chromatographic process, ensuring optimal column performance.</li><li>Gradient Capability: Two or up to four P224 pumps offer gradient capabilities, allowing for the creation of complex elution profiles controlled from PC to enhance separation efficiency.</li><li>User-Friendly Interface: An intuitive and user-friendly interface facilitates easy operating and monitoring of the pump parameters, contributing to efficient workflow in the laboratory.</li><li>Compatibility: P224 is compatible with a variety of chromatography instruments and stationary phases commonly used in semipreparative applications.</li><li>Reliability and Durability: A robust construction and high-quality materials ensure the pump’s durability and reliability, minimizing downtime and maintenance requirements.</li><li>Precision in Dosing: P224 design prioritizes accuracy in dosing, making it suitable for applications where precise control over sample injection and elution volumes is essential.</li><li>Safety Features: Incorporation of safety features such as pressure sensors, and overpressure protection ensures the well-being of both the operator and the equipment.</li><li>Maintenance and Calibration: P224 pumps are easy to maintain and calibrate, with clear documentation provided by us for routine upkeep and troubleshooting.</li><li>Versatility: P224 supports a wide range of solvents and mobile phases commonly used in chromatography, offering flexibility for various applications.</li><li>Data Logging and Connectivity: The capability to log service data enhances operation traceability and documentation.</li></ul><h3>TECHNICAL SPECIFICATION</h3><ul><li>Flow Rates: 0,1 ml/min – 25,00 ml/min with step 0.1 ml/min (semiprep)</li><li>Pressure: 30 MPa/4300 psi</li><li>Flow precision: &nbsp;&nbsp;&nbsp;&nbsp;± 0,2% RSD @ 1mL/min. and 7 Mpa</li><li>Flow accuracy: ± 1% @ 1mL/min. and 7 Mpa</li><li>Pressure accuracy: ± 2% of full-scale, with 80:20 H2O/IPA @ 7 Mpa</li><li>Pulsation: better than ± 0.5% @ 1mL/min. and 7 MPa</li><li>Remote control: USB</li><li>I/O connector: Start, Stop, Status, Error</li></ul>You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/pumps/preparative-pumps/p224-pump-dual-head-stainless-steel/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

KNAUER AZURA® Compact Prep HPLC System

The AZURA® Prep Compact system is the perfect start into preparative chromatography. With the complete, semi-preparative HPLC system you master your isocratic purification tasks.

AZURA® Preparative HPLC Customized purificationAZURA® preparative systems are the perfect solution for frequently changing separation tasks – from milligram to kilogram scale. Design your AZURA preparative system to your needs and combine flexibility and reliability.<h5>Customaze your system</h5>AZURA® Prep systems are tailor-made for you. Configure your system from injection to detection and choose between different materials, flow rates, valves, and detectors.<h5>Easy scale up from compact to pilot systems</h5>Due to the flexible design of our devices, you can easily change parts like pump heads or flow cells and integrate all components of the compact into the pilot-scale system.<h5>Stacked injections, peak &amp; solvent recycling</h5>AZURA® Prep systems can be used for special separation modes like peak recycling and stacked injections. We help you to configure your system and choose the best software for you.<h3>Flexibility and performance</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Prep_HPLC_System_90fef641c3.jpg" alt="KNAUER AZURA® Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Prep_HPLC_System_90fef641c3.jpg 245w," sizes="100vw" width="1920" height="1059"><h2>AZURA® Compact Prep HPLC System</h2>The AZURA® Prep Compact system is the perfect start into preparative chromatography. With the complete, semi-preparative HPLC system you master your isocratic purification tasks.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Compact_Prep_HPLC_System_3_863a93178e.jpg" alt="KNAUER: AZURA® Compact Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Compact_Prep_HPLC_System_3_863a93178e.jpg 245w," sizes="100vw" width="1590" height="604"><h4>Compact Prep System</h4>One manual injection can purify several hundred milligrams at up to 50 ml/min. Detection takes place via a versatile UV/VIS detector. The intuitive preparative software PurityChrom controls the compact system and regulates the fraction collection via a 12-port fractionating valve. Thanks to its compact design, the AZURA Prep Compact system finds its place in every laboratory.<img style="aspect-ratio:1024/742;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Compact_Prep_HPLC_System_48291f6f6e.webp" alt="KNAUER: AZURA® Compact Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Compact_Prep_HPLC_System_48291f6f6e.webp 215w," sizes="100vw" width="1024" height="742"><ul><li>Up to 150 bar</li><li>Complete semi-preparative isocratic HPLC system with low space requirements</li><li>Up to 10 ml injection volumes &nbsp;</li><li>Injection valve incl. 500 μl sample loop&nbsp;</li><li>Collect up to 16 large fractions</li><li>Columns from 4–20 mm ID</li><li>UV/VIS detector with one variable wavelength</li><li>Intuitive PurityChrom®, MobileControl® software</li><li>Compact and expandable</li></ul><h3>Easy upgrade without big investment</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Compact_Prep_HPLC_System_4_efed81c462.jpg" alt="KNAUER: AZURA® Compact Prep HPLC System: Easy upgrade without big investment." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Compact_Prep_HPLC_System_4_efed81c462.jpg 245w," sizes="100vw" width="1600" height="761"><h4>Pilot Prep System</h4><div><div data-message-author-role="assistant" data-message-id="efe48b67-eccf-4ebf-8484-ea6d1f96ed57" dir="auto" data-message-model-slug="gpt-4o"><div><div>After starting preparative chromatography with the space-saving prep system, the requirements for your purification tasks can quickly increase. The existing Compact System (50 ml/min) can be expanded to a Pilot System (220 ml/min) by investing in a fraction collector and a preparative pump. All components of the Compact System are fully integrated into the Pilot System.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Compact_Prep_HPLC_System_2_d713ad0086.jpg" alt="KNAUER: AZURA® Compact Prep HPLC System: Easy upgrade without big investment." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Compact_Prep_HPLC_System_2_d713ad0086.jpg 178w," sizes="100vw" width="1606" height="1407"><ul><li>Pilot Ternary gradient HPLC system with injection valve incl. 500 μl sample loop&nbsp;</li><li>Sample pump with automatic sample selection&nbsp;</li><li>UV/VIS detector&nbsp;with one variable wavelength</li><li>Intuitive PurityChrom® software</li><li>Fraction collector</li></ul></div></div></div></div>

KNAUER AZURA® Bio Compact AC FPLC System

The AZURA Compact AC system qualifies for fast and reliable affinity chromatography. 

KNAUER protein purification: The flexible FPLC platformAZURA® Bio purification systems&nbsp;complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability. The biocompatible/metal-free FPLC is the perfect choice for your protein purification task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg" alt="KNAUER: AZURA® Bio Compact AC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg 245w," sizes="100vw" width="1506" height="600">Design your AZURA Bio purification system to fit your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection, and fraction collection enable automation of the purification process.A wide range of detectors ensures the visibility of target molecules. Various flow rates and compatibility with columns from all vendors provide maximum flexibility. The intuitive PurityChrom® software integrates all the advantages of a versatile purification system.<h3>AZURA® Bio Compact AC FPLC System</h3><h4>For affinity chromatography</h4>The AZURA Compact AC system qualifies for fast and reliable affinity chromatography. Select your sample, washing, and elution buffer using the selection valve. Your proteins of interest are detected by UV and automatically collected via the fractionation valve.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_4_bef2309e7d.jpg" alt="KNAUER: AZURA® Bio Compact AC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_4_bef2309e7d.jpg 245w," sizes="100vw" width="1666" height="513"><h3>Key features</h3><ul><li data-start="93" data-end="153">Flow rate: 0.01–50 ml/min (recommended: 1–40 ml/min)</li><li data-start="154" data-end="193">Maximum system pressure: 50 bar</li><li data-start="194" data-end="278">Sample and buffer selection: Automatic valve for up to 12 buffers or samples</li><li data-start="279" data-end="349">Detection: Variable single wavelength UV detector (190–500 nm)</li><li data-start="350" data-end="429">Column compatibility: Compatible with affinity columns from all vendors</li><li data-start="430" data-end="574">Fraction collection: 16-port fractionation valve, capable of collecting up to 16 fractions for flow-through, wash, and elution fractions</li><li data-start="575" data-end="663">Software: Intuitive and powerful PurityChrom® software, 21 CFR Part 11 compliant</li><li data-start="664" data-end="737">Affinity purification: Designed for tagged proteins or antibodies</li><li data-start="738" data-end="867">Compact system: User-friendly and cost-efficient, with the ability to upgrade for more complex protein purification tasks</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_5_17f4f13731.jpg" alt="KNAUER AZURA® Bio Compact AC FPLC System: Protein purification based on high affinity Chromatogram &amp; Legend." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_5_17f4f13731.jpg 209w," sizes="100vw" width="1024" height="766">&nbsp;

KNAUER AZURA® Bio Compact SEC FPLC System

AZURA Compact SEC systems handle time-consuming SEC methods in your lab without occupying your valuable FPLC system.

KNAUER protein purification: The flexible FPLC platformAZURA® Bio purification systems&nbsp;complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability. The biocompatible/metal-free FPLC is the perfect choice for your protein purification task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg" alt="KNAUER: AZURA® Bio Compact AC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg 245w," sizes="100vw" width="1506" height="600">Design your AZURA Bio purification system to fit your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection, and fraction collection enable automation of the purification process.A wide range of detectors ensures the visibility of target molecules. Various flow rates and compatibility with columns from all vendors provide maximum flexibility. The intuitive PurityChrom® software integrates all the advantages of a versatile purification system.<h3>AZURA® Bio Compact SEC FPLC System</h3><h4>For Size Exclusion Chromatography (SEC)</h4>AZURA Compact SEC systems take over time-consuming SEC methods in your lab without blocking your valuable FPLC system. Due to its compact design and intuitive FPLC software PurityChrom®, the system offers outstanding performance and ease of use.&nbsp;Pre-designed methods are included in the software for the designated SEC system and can be easily adapted by changing the column volume. AZURA Compact SEC supports all columns available on the market.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_SEC_FPLC_System_4_f5efb08061.jpg" alt="KNAUER: AZURA® Bio Compact SEC FPLC System " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_SEC_FPLC_System_4_f5efb08061.jpg 245w," sizes="100vw" width="1358" height="540"><h3>Key features</h3><ul><li data-start="93" data-end="157">Flow rate: 0.001–10 ml/min (recommended: 0.1–8.0 ml/min)</li><li data-start="158" data-end="197">Maximum system pressure: 50 bar</li><li data-start="198" data-end="276">Injection system: Injection valve for sample injection via sample loop</li><li data-start="277" data-end="347">Detection: Variable single wavelength UV detector (190–500 nm)</li><li data-start="348" data-end="423">Fraction collection: Fraction collector for automated fractionation</li><li data-start="424" data-end="522">Column compatibility: Supports columns from all vendors, including third-party SEC columns</li><li data-start="523" data-end="611">Software: Intuitive and powerful PurityChrom® software, 21 CFR Part 11 compliant</li><li data-start="612" data-end="780">System design:<ul data-start="637" data-end="780"><li data-start="637" data-end="666">Isocratic buffer delivery</li><li data-start="669" data-end="687">Loop injection</li><li data-start="690" data-end="734">Wide range of collection racks available</li><li data-start="737" data-end="780">Specially designed for SEC applications</li></ul></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_SEC_FPLC_System_3_7a4bc1f60b.jpg" alt="KNAUER: AZURA® Bio Compact SEC FPLC System " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_SEC_FPLC_System_3_7a4bc1f60b.jpg 245w," sizes="100vw" width="1920" height="1152">Don't use your valuable gradient FPLC system for boring and time-consuming size exclusion separations. Choose your dedicated AZURA SEC system.<figure class="image"><img style="aspect-ratio:690/490;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_SEC_FPLC_System_45d5079064.webp" alt="KNAUER: AZURA® Bio Compact SEC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_SEC_FPLC_System_45d5079064.webp 220w," sizes="100vw" width="690" height="490"></figure>With its compact design and intuitive software, the system offers excellent performance, ease of use and supports all SEC columns available on the market.​

KNAUER AZURA® Bio Lab FPLC System

AZURA Bio Lab allows you to create FPLC systems with highest independence. Just pick your modules and build-up the system yourself. The intuitive software PurityChrom will be flexibly adjusted to your system.

KNAUER protein purification: The flexible FPLC platformAZURA® Bio purification systems&nbsp;complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability. The biocompatible/metal-free FPLC is the perfect choice for your protein purification task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg" alt="KNAUER: AZURA® Bio Compact AC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg 245w," sizes="100vw" width="1506" height="600">Design your AZURA Bio purification system to fit your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection, and fraction collection enable automation of the purification process.A wide range of detectors ensures the visibility of target molecules. Various flow rates and compatibility with columns from all vendors provide maximum flexibility. The intuitive PurityChrom® software integrates all the advantages of a versatile purification system.<h3>AZURA® Bio Lab FPLC System</h3><h4>R&amp;D-scale gradient system for complex purifications of bio molecules</h4>AZURA Bio Lab allows you to create FPLC systems with highest independence. Just pick your modules and build-up the system yourself. The intuitive software PurityChrom will be flexibly adjusted to your system.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_FPLC_System_3_7b22a3e099.jpg" alt="KNAUER: AZURA® Bio Lab FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_FPLC_System_3_7b22a3e099.jpg 245w," sizes="100vw" width="1645" height="671"><h3>Key features</h3><ul><li data-start="93" data-end="145">Flow rate: 0.001–50 ml/min (maximum 240 bar)</li><li data-start="146" data-end="224">Injection system: Sample injections via sample loop and/or sample pump</li><li data-start="225" data-end="295">Detection: Variable single wavelength UV detector (190–500 nm)</li><li data-start="296" data-end="386">Fraction collection: XY fraction collector for large volumes or numerous fractions</li><li data-start="387" data-end="493">Column compatibility: Supports up to 7 columns for method development – all FPLC columns supported</li><li data-start="494" data-end="584">Suitable for Ion-exchange, HILIC, affinity, SEC, and RP</li><li data-start="585" data-end="640">Reproducible and automated protein purification</li><li data-start="641" data-end="695">System usability: Suitable for cold room usage</li><li data-start="696" data-end="820">Software:<ul data-start="716" data-end="820"><li data-start="716" data-end="780">Intuitive and powerful PurityChrom® software (basic license)</li><li data-start="783" data-end="820">21 CFR Part 11 compliant software</li></ul></li><li data-start="821" data-end="924">Additional features:<ul data-start="852" data-end="924"><li data-start="852" data-end="924">Conductivity and pH-monitoring, pressure, and air sensors (optional)</li></ul></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_FPLC_System_4740938659.jpg" alt="KNAUER: AZURA® Bio Lab FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_FPLC_System_4740938659.jpg 235w," sizes="100vw" width="1920" height="1279">From simple to advanced, from lab to process scale – configure your FPLC with our modular design according to your purification needs.Designed and optimized for all FPLC applications such as affinity, ion exchange, hydrophobic interaction and size exclusion chromatography. All system modules can be expanded with additional modules.

KNAUER AZURA® Bio Lab Advanced FPLC System

Gradient-capable FPLC system for a wide range of purifications including automated two-step applications

KNAUER protein purification: The flexible FPLC platformAZURA® Bio purification systems&nbsp;complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability. The biocompatible/metal-free FPLC is the perfect choice for your protein purification task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg" alt="KNAUER: AZURA® Bio Compact AC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg 245w," sizes="100vw" width="1506" height="600">Design your AZURA Bio purification system to fit your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection, and fraction collection enable automation of the purification process.A wide range of detectors ensures the visibility of target molecules. Various flow rates and compatibility with columns from all vendors provide maximum flexibility. The intuitive PurityChrom® software integrates all the advantages of a versatile purification system.<h3>AZURA® Bio Lab Advanced FPLC System</h3><h4>Gradient-capable FPLC system for a wide range of purifications including automated two-step applications</h4>Protein purification involves most of the times two to three steps:<ol><li>capture step</li><li>optional intermediated step</li><li>polishing step</li></ol>The transition from one to another step generally involves manual interaction and thus is time-consuming. Automation by combining these steps increases the efficiency and optimizes the workflow.&nbsp;The quick and automated linkage of multiple chromatographic purification steps into one method eliminates manual sample handling and minimizes time spent between steps. This automation strategy can be easily adapted to each purification task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_FPLC_System_3_7b22a3e099.jpg" alt="KNAUER: AZURA® Bio Lab Advanced FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_FPLC_System_3_7b22a3e099.jpg 245w," sizes="100vw" width="1645" height="671"><h3>Key features</h3><ul data-start="93" data-end="1077"><li data-start="93" data-end="144">Flow rate: 0.001–50 ml/min (maximum 50 bar)</li><li data-start="145" data-end="263">Injection system: Sample injection via loop or sample pump, with automated storage and reinjection of proteins</li><li data-start="264" data-end="433">Detection system:<ul data-start="292" data-end="433"><li data-start="292" data-end="320">Multichannel UV detector</li><li data-start="323" data-end="405">Highly sensitive multi-wavelength detector for proteins, DNA/RNA, and peptides</li><li data-start="408" data-end="433">Conductivity detector</li></ul></li><li data-start="434" data-end="566">Fraction collection: XY fraction collector with outlet valve for automated collection of large volumes or numerous fractions</li><li data-start="567" data-end="673">Column compatibility: Supports up to 7 columns for method development – all FPLC columns supported</li><li data-start="674" data-end="764">Suitable for Ion-exchange, HILIC, affinity, SEC, and RP</li><li data-start="765" data-end="864">Automation: Enables challenging protein purification tasks with minimal manual intervention</li><li data-start="865" data-end="973">Software:<ul data-start="885" data-end="973"><li data-start="885" data-end="933">Intuitive and powerful PurityChrom® software</li><li data-start="936" data-end="973">21 CFR Part 11 compliant software</li></ul></li><li data-start="974" data-end="1077">Additional features:<ul data-start="1005" data-end="1077"><li data-start="1005" data-end="1077">Conductivity and pH-monitoring, pressure, and air sensors (optional)</li></ul></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_Advanced_FPLC_System_3_9cf517ceaf.webp" alt="KNAUER: AZURA® Bio Lab Advanced FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_Advanced_FPLC_System_3_9cf517ceaf.webp 234w," sizes="100vw" width="690" height="460"><h3>Two-step purification: Special multi-column chromatography solutions</h3>Designed and optimized for all FPLC applications such as affinity, ion exchange, hydrophobic interaction and size exclusion chromatography. In addition, you can perform dedicated twoSTEP applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_Advanced_FPLC_System_44bff80cfc.webp" alt="KNAUER: AZURA® Bio Lab Advanced FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_Advanced_FPLC_System_44bff80cfc.webp 209w," sizes="100vw" width="1920" height="1434"><h3>Automated two-step purification of mouse IgG antibodies</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_Advanced_FPLC_System_4_c86d2177ce.jpg" alt="KNAUER: AZURA® Bio Lab Advanced FPLC System: The affinity chromatography step was automatically combined with a gel filtration step to exchange the buffer of the purified mouse IgG antibodies; Phase 1: Column equilibration, Phase 2: Sample injection and washing, Phase 3: Elution of IgG from protein A column, Phase 4: Desalting of IgG." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_Advanced_FPLC_System_4_c86d2177ce.jpg 245w," sizes="100vw" width="1085" height="605">

KNAUER AZURA® Bio Lab Pilot FPLC System

Configure your FPLC system based on your purification requirements. The Pilot system is designed for scale-up and process applications. All system modules can be expanded with additional modules.

KNAUER protein purification: The flexible FPLC platformAZURA® Bio purification systems&nbsp;complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability. The biocompatible/metal-free FPLC is the perfect choice for your protein purification task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg" alt="KNAUER: AZURA® Bio Compact AC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg 245w," sizes="100vw" width="1506" height="600">Design your AZURA Bio purification system to fit your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection, and fraction collection enable automation of the purification process.A wide range of detectors ensures the visibility of target molecules. Various flow rates and compatibility with columns from all vendors provide maximum flexibility. The intuitive PurityChrom® software integrates all the advantages of a versatile purification system.<h3>AZURA® Bio Lab Pilot FPLC System</h3><h4>High Flow Bio Purification FPLC System – up to 1000 ml/min</h4>Configure your FPLC system based on your purification requirements. The Pilot system is designed for scale-up and process applications. All system modules can be expanded with additional modules.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_Pilot_FPLC_System_e208baf41d.jpg" alt="KNAUER: AZURA® Bio Lab Pilot FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_Pilot_FPLC_System_e208baf41d.jpg 156w," sizes="100vw" width="1000" height="1000"><h3>Key features</h3><ul data-start="93" data-end="1069"><li data-start="93" data-end="129">Flow rate: 0.001–1000 ml/min</li><li data-start="130" data-end="192">Injection system: Sample pump for large sample volumes</li><li data-start="193" data-end="351">Detection system:<ul data-start="221" data-end="351"><li data-start="221" data-end="276">Variable single wavelength UV detector (190–500 nm)</li><li data-start="279" data-end="351">Conductivity and pH-monitoring, pressure, and air sensors (optional)</li></ul></li><li data-start="352" data-end="465">Fraction collection: XY fraction collector for reliable collection of large volumes or numerous fractions</li><li data-start="466" data-end="559">Suitable for SEC, AC, IEX, and HIC, with upscaling capabilities</li><li data-start="560" data-end="651">Automation: High-flow buffer delivery and mixing for large-scale purification tasks</li><li data-start="652" data-end="739">System design: Same footprint as <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1625">AZURA Lab FPLC systems</a> for compact scalability</li><li data-start="740" data-end="871">Software:<ul data-start="760" data-end="871"><li data-start="760" data-end="831">Intuitive and powerful PurityChrom® software in basic configuration</li><li data-start="834" data-end="871">21 CFR Part 11 compliant software</li></ul></li><li data-start="872" data-end="1069">Scalability:<ul data-start="895" data-end="1069"><li data-start="895" data-end="965">Configure your FPLC system based on your purification requirements</li><li data-start="968" data-end="1069">Scale-up is possible with the same flexibility and software while maintaining a minimal footprint</li></ul></li></ul>

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