LCMS
More information
WebinarsAbout usContact usTerms of use
LabRulez s.r.o. All rights reserved. Content available under a CC BY-SA 4.0 Attribution-ShareAlike
Manufacturer
KNAUER
KNAUER
Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.
Distributor
KNAUER
KNAUER manufactures high-end scientific instruments for research, routine analysis, quality assurance, and other applications.
KNAUER AZURA® Bio Lab Advanced FPLC SystemGradient-capable FPLC system for a wide range of purifications including automated two-step applications
Features

KNAUER AZURA® Bio Lab Advanced FPLC System

KNAUER protein purification: The flexible FPLC platform

AZURA® Bio purification systems 

complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability. The biocompatible/metal-free FPLC is the perfect choice for your protein purification task.

KNAUER: AZURA® Bio Compact AC FPLC System.KNAUER: AZURA® Bio Compact AC FPLC System.

Design your AZURA Bio purification system to fit your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection, and fraction collection enable automation of the purification process.

A wide range of detectors ensures the visibility of target molecules. Various flow rates and compatibility with columns from all vendors provide maximum flexibility. The intuitive PurityChrom® software integrates all the advantages of a versatile purification system.

AZURA® Bio Lab Advanced FPLC System

Gradient-capable FPLC system for a wide range of purifications including automated two-step applications

Protein purification involves most of the times two to three steps:

  1. capture step
  2. optional intermediated step
  3. polishing step

The transition from one to another step generally involves manual interaction and thus is time-consuming. Automation by combining these steps increases the efficiency and optimizes the workflow. 

The quick and automated linkage of multiple chromatographic purification steps into one method eliminates manual sample handling and minimizes time spent between steps. This automation strategy can be easily adapted to each purification task.

KNAUER: AZURA® Bio Lab Advanced FPLC System.KNAUER: AZURA® Bio Lab Advanced FPLC System.

Key features

  • Flow rate: 0.001–50 ml/min (maximum 50 bar)
  • Injection system: Sample injection via loop or sample pump, with automated storage and reinjection of proteins
  • Detection system:
    • Multichannel UV detector
    • Highly sensitive multi-wavelength detector for proteins, DNA/RNA, and peptides
    • Conductivity detector
  • Fraction collection: XY fraction collector with outlet valve for automated collection of large volumes or numerous fractions
  • Column compatibility: Supports up to 7 columns for method development – all FPLC columns supported
  • Suitable for Ion-exchange, HILIC, affinity, SEC, and RP
  • Automation: Enables challenging protein purification tasks with minimal manual intervention
  • Software:
    • Intuitive and powerful PurityChrom® software
    • 21 CFR Part 11 compliant software
  • Additional features:
    • Conductivity and pH-monitoring, pressure, and air sensors (optional)

KNAUER: AZURA® Bio Lab Advanced FPLC System.KNAUER: AZURA® Bio Lab Advanced FPLC System.

Two-step purification: Special multi-column chromatography solutions

Designed and optimized for all FPLC applications such as affinity, ion exchange, hydrophobic interaction and size exclusion chromatography. In addition, you can perform dedicated twoSTEP applications.

KNAUER: AZURA® Bio Lab Advanced FPLC System.KNAUER: AZURA® Bio Lab Advanced FPLC System.

Automated two-step purification of mouse IgG antibodies

KNAUER: AZURA® Bio Lab Advanced FPLC System: The affinity chromatography step was automatically combined with a gel filtration step to exchange the buffer of the purified mouse IgG antibodies; Phase 1: Column equilibration, Phase 2: Sample injection and washing, Phase 3: Elution of IgG from protein A column, Phase 4: Desalting of IgG.KNAUER: AZURA® Bio Lab Advanced FPLC System: The affinity chromatography step was automatically combined with a gel filtration step to exchange the buffer of the purified mouse IgG antibodies; Phase 1: Column equilibration, Phase 2: Sample injection and washing, Phase 3: Elution of IgG from protein A column, Phase 4: Desalting of IgG.

Manufacturer
KNAUER
KNAUER
Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.
Distributor
KNAUER
KNAUER manufactures high-end scientific instruments for research, routine analysis, quality assurance, and other applications.
Other projects
GCMS
ICPMS
Follow us
More information
WebinarsAbout usContact usTerms of use
LabRulez s.r.o. All rights reserved. Content available under a CC BY-SA 4.0 Attribution-ShareAlike