<p><strong>🎤Amanda Guiraldelli Mahr&nbsp;</strong></p><p><strong>Amanda shares insights from her work across small molecules, peptides, proteins, and biopharmaceuticals, including her experience with LC-MS, metabolomics, impurity analysis, and oligonucleotide therapeutics.</strong></p><h4><strong>What You’ll Learn in This Episode:</strong></h4><ul><li>What AQbD actually means (in simple terms)</li><li>How DOE differs from traditional one-factor-at-a-time experiments</li><li>Why modern labs are shifting toward predictive, data-driven method development</li><li>The biggest benefits of DOE: more insight with fewer experiments*</li><li>Real-world example: optimizing oligonucleotide impurity separations using AQbD&nbsp;</li><li>Key LC parameters that impact performance (pH, gradient, column, temperature)</li><li>Challenges of working with large molecules vs. small molecules</li><li>Tools and software for DOE and chromatographic modeling</li><li>How AI and modeling are shaping the future of chromatography</li></ul><blockquote><p><strong>Key Takeaway: </strong>“Shift your mindset from testing to understanding.”</p></blockquote><h4><strong>Who This Episode Is For:</strong></h4><ul><li>Undergraduate and graduate chemistry students</li><li>Analytical scientists and method developers</li><li>Pharma &amp; biopharma professionals</li><li>Anyone working with LC, LC-MS, or chromatography method development</li></ul><h3><strong>Video Transcription</strong></h3><p>In this episode of <i>Concentrating on Chromatography</i>, David interviews <strong>Amanda Guiraldelli Mahr</strong>, an analytical chemist whose career has spanned pharmaceuticals, metabolomics, regulatory science, mass spectrometry, and biopharmaceutical analysis. Amanda discusses her journey from pharmacy and biochemistry studies to working with chromatography and mass spectrometry in academic, regulatory, and industrial environments.</p><p>The conversation focuses on the growing role of <strong>Analytical Quality by Design (AQbD)</strong>, <strong>Design of Experiments (DOE)</strong>, predictive modeling, and the future integration of artificial intelligence into chromatographic method development. Amanda also shares practical insights into method development for small molecules, peptides, proteins, oligonucleotides, and emerging therapeutics.</p><h4><strong>Early Career and Introduction to Chromatography</strong></h4><p>Amanda's interest in analytical chemistry began during her studies in pharmacy and biochemistry. An internship focused on chromatography for botanical medicine analysis introduced her to separation science and inspired her to pursue a PhD in analytical chemistry.</p><p>During her doctoral research, she worked extensively with <strong>LC-MS-based metabolomics</strong>, which led her to explore experimental design approaches and method development strategies. Her passion for chromatography and mass spectrometry deepened further while working at the United States Pharmacopeia (USP), where she routinely applied chromatographic and mass spectrometric techniques to characterize:</p><ul><li>Small-molecule drug substances</li><li>Pharmaceutical ingredients</li><li>Dietary supplements</li><li>Botanical products</li><li>Peptides</li><li>Proteins</li></ul><p>Her work increasingly involved understanding chromatographic separation mechanisms through experimental design and modeling approaches.</p><h4><strong>Expanding into Proteomics, Nitrosamines, and Biopharmaceuticals</strong></h4><p>Amanda's scientific curiosity led her beyond traditional small-molecule analysis.</p><p>Throughout her career she gained experience in:</p><ul><li>High-resolution mass spectrometry</li><li>Structural elucidation</li><li>Proteomics</li><li>Bioanalysis</li><li>Trace impurity analysis</li><li>Nitrosamine monitoring</li><li>Oligonucleotide therapeutics</li><li>Large proteins and peptides</li></ul><p>She worked with advanced fragmentation techniques including:</p><ul><li>CID (Collision-Induced Dissociation)</li><li>HCD (Higher-Energy Collisional Dissociation)</li><li>UV Photodissociation</li></ul><p>Later, she became involved in developing highly sensitive LC-MS and GC-MS methods for monitoring <strong>nitrosamines</strong>, a major regulatory concern due to their genotoxic potential. She also trained regulatory agencies in mass spectrometry applications and method validation before moving into biopharmaceutical research and contract research activities focused on proteins, peptides, oligonucleotides, and RNA therapeutics.</p><h4><strong>What Does a Chromatographer Do Every Day?</strong></h4><p>When asked to summarize the day-to-day work of a chromatographer, Amanda highlighted three core activities:</p><ul><li>Designing experiments</li><li>Troubleshooting analytical methods</li><li>Interpreting unexpected results</li></ul><p>She emphasized that a large portion of her work involves data analysis and learning from experimental outcomes in order to optimize methods and better understand chromatographic behavior.</p><h4><strong>Understanding Analytical Quality by Design (AQbD)</strong></h4><p>One of the central topics of the discussion was <strong>Analytical Quality by Design (AQbD)</strong>.</p><p>Amanda describes AQbD as a systematic approach for understanding how analytical methods perform and identifying factors that influence method variability.</p><p>Key objectives of AQbD include:</p><ul><li>Identifying critical method parameters</li><li>Understanding sources of variability</li><li>Building robustness into methods from the beginning</li><li>Improving method reliability throughout its lifecycle</li></ul><p>Rather than correcting problems after implementation, AQbD focuses on proactively designing analytical procedures that consistently deliver high-quality results.</p><p>Amanda noted that AQbD principles are strongly linked to pharmaceutical quality frameworks established through ICH guidelines such as:</p><ul><li>ICH Q8 (Pharmaceutical Development)</li><li>ICH Q14 (Analytical Procedure Development)</li></ul><p>These guidelines have significantly influenced modern pharmaceutical method development practices.</p><h4><strong>AQbD vs. Traditional Trial-and-Error Development</strong></h4><p>Amanda contrasted AQbD with the traditional <strong>one-factor-at-a-time (OFAT)</strong> approach.</p><h5><strong>Traditional Approach</strong></h5><ul><li>Changes one parameter at a time</li><li>Provides limited understanding of interactions</li><li>Often misses important relationships between variables</li></ul><h5><strong>AQbD Approach</strong></h5><ul><li>Uses multivariate experimental designs</li><li>Studies multiple variables simultaneously</li><li>Reveals interactions between parameters</li><li>Enables predictive modeling</li><li>Defines robust operating ranges</li></ul><p>According to Amanda, AQbD provides significantly more knowledge about method behavior while reducing unnecessary experimentation.</p><h4><strong>Design of Experiments (DOE): A Core AQbD Tool</strong></h4><p>Amanda clarified an important misconception:</p><p><strong>DOE is not the same as AQbD.</strong></p><p>Instead:</p><ul><li>AQbD is a broader philosophy and lifecycle approach.</li><li>DOE is one of the most powerful tools used within AQbD.</li></ul><p>DOE helps scientists:</p><ul><li>Identify critical factors</li><li>Evaluate interactions</li><li>Map regions of good and poor performance</li><li>Support risk assessment</li><li>Build predictive models</li></ul><p>However, AQbD also includes risk management, control strategies, and lifecycle monitoring beyond experimental design itself.</p><h4><strong>Practical Advice for Scientists New to DOE</strong></h4><p>For scientists interested in implementing DOE, Amanda recommends starting with simple screening studies.</p><p>Examples include evaluating:</p><ul><li>LC column chemistry</li><li>Mobile-phase pH</li><li>Organic modifiers</li><li>Temperature</li></ul><p>Important responses to monitor may include:</p><ul><li>Resolution</li><li>Peak tailing</li><li>Selectivity</li><li>Efficiency</li></ul><p>She encourages scientists to focus not only on collecting data but on understanding the patterns revealed by experimental results. As experience grows, more advanced optimization studies and predictive models can be developed.</p><h4><strong>Which LC Parameters Matter Most?</strong></h4><p>Amanda emphasized that critical parameters depend on:</p><ul><li>Sample type</li><li>Separation mode</li><li>Intended method purpose</li><li>Development stage</li></ul><p>For reversed-phase LC, common screening parameters include:</p><ul><li>Column chemistry</li><li>Mobile-phase pH</li><li>Organic solvent composition</li><li>Temperature</li></ul><p>For ion-pair chromatography, additional factors such as:</p><ul><li>Ion-pairing reagent type</li><li>Ion-pairing reagent concentration</li></ul><p>can strongly influence selectivity and retention behavior.</p><p>She stresses that proper risk assessment should always guide parameter selection.</p><h4><strong>Case Study: Oligonucleotide Impurity Profiling</strong></h4><p>Amanda shared an example involving impurity analysis of <strong>oligonucleotide therapeutics</strong>.</p><p>One major challenge is that impurities are often structurally very similar to the active pharmaceutical ingredient (API), making chromatographic separation difficult.</p><p>Using AQbD and DOE, her team systematically evaluated:</p><ul><li>LC column chemistries</li><li>Ion-pairing reagents</li><li>Temperature</li><li>Gradient conditions</li></ul><p>A desirability-function approach allowed simultaneous optimization of multiple critical resolutions rather than focusing on a single impurity pair.</p><p>This strategy enabled the development of an LC-UV method capable of separating impurities that might otherwise require LC-MS for quantification.</p><h4><strong>The Challenges of Large Molecules</strong></h4><p>Amanda identifies large biomolecules as some of the most challenging analytes in chromatography.</p><p>Compared with small molecules, proteins and oligonucleotides exhibit:</p><ul><li>Slower mass transfer kinetics</li><li>Peak broadening</li><li>Reduced sensitivity</li><li>Greater sensitivity to system-related factors</li></ul><p>Even seemingly minor differences can affect performance, including:</p><ul><li>Dwell volume</li><li>Tubing dimensions</li><li>System dispersion</li></ul><p>She also highlighted non-specific adsorption as a major challenge.</p><p>Examples include:</p><h5><strong>Oligonucleotides</strong></h5><p>Negatively charged phosphate backbones can interact strongly with metal surfaces, reducing recovery and reproducibility.</p><h5><strong>Peptides</strong></h5><p>Basic amino acid residues may interact with residual silanol groups on silica-based stationary phases, affecting chromatographic performance.</p><p>These challenges have driven innovation in:</p><ul><li>Low-adsorption LC systems</li><li>Surface-modified columns</li><li>Hybrid particle technologies</li><li>Improved consumables and hardware design</li></ul><h4><strong>Software and Modeling Tools</strong></h4><p>Amanda discussed several software platforms commonly used for DOE and method optimization:</p><h5><strong>DOE Software</strong></h5><ul><li>Design-Expert</li><li>JMP</li><li>Minitab</li><li>MODDE</li></ul><h5><strong>Chromatographic Modeling Software</strong></h5><ul><li>DryLab</li><li>ACD/AutoChrom</li><li>ChromSword</li></ul><p>She also noted that modern software increasingly integrates directly with chromatographic data systems, enabling automated experiment execution and data analysis.</p><h4><strong>Essential Skills Beyond Instrument Operation</strong></h4><p>According to Amanda, successful chromatographers need more than technical instrument expertise.</p><p>Key skills include:</p><ul><li>Chromatographic fundamentals</li><li>Statistics</li><li>Chemometrics</li><li>Data interpretation</li><li>Predictive modeling</li><li>Risk assessment</li></ul><p>She emphasized that statistical literacy is particularly important for implementing AQbD principles and validating predictive models.</p><h4><strong>The Future of Chromatography</strong></h4><p>Looking ahead, Amanda is particularly excited about the integration of:</p><ul><li>Artificial intelligence</li><li>Predictive modeling</li><li>Knowledge-driven method development</li></ul><p>She believes these technologies will help scientists better understand separation mechanisms and accelerate development of methods for increasingly complex therapeutics.</p><p>Ultimately, she expects analytical science to play an even more central role in pharmaceutical development, manufacturing, and quality assurance.</p><h4><strong>Conclusion</strong></h4><p>Amanda's career illustrates how chromatography has evolved from traditional small-molecule analysis into a highly interdisciplinary field spanning proteomics, biopharmaceuticals, oligonucleotide therapeutics, and advanced regulatory science. Her experience demonstrates the value of combining chromatographic expertise with AQbD principles, DOE, risk assessment, and predictive modeling.</p><p>As analytical challenges continue to grow in complexity, approaches that emphasize understanding, prediction, and quality by design are becoming increasingly important. The future of chromatography is likely to be shaped by data-driven decision making, advanced modeling, and artificial intelligence—tools that will enable scientists to develop more robust, efficient, and reliable analytical methods.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

AQbD Explained: How Design of Experiments is Transforming Chromatography

Untargeted Metabolomics Identifies N-Lactoyl-Amino Acids as Dose-Responsive Plasma Biomarkers of Metformin Adherence in Type 2 Diabetes

<p><strong>In the week of June 22, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Simplifying Complexity in GLP-1 Receptor Agonist Analysis: From Structural Characterization to Impurity Monitoring</strong></h5><ul><li>Tu, 23.6.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6275">Registration</a></li></ul><p>Simplify GLP-1 receptor agonist analysis. Learn how a streamlined LC-MS workflow combines UHPLC and high-resolution MS for peptide characterization and impurity monitoring.</p><h5><strong>2. Tosoh Bioscience: Tosoh Tuesday - Exploring the AAV Toolkit</strong></h5><ul><li>Tu, 23.6.2026 15:45 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6273">Registration</a></li></ul><p>Improve AAV analysis and purification. Learn how to increase recovery and purity using the TOYOPEARL GigaCap Q-650S resin and columns from Tosoh Bioscience.</p><h5><strong>3. Phenomenex: Sustainable Strategies for High Sensitivity Analysis for Clinical Research LC-MS/MS Methods</strong></h5><ul><li>We, 24.6.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6278">Registration</a></li></ul><p>Optimize clinical LC-MS/MS methods using sustainable ethyl acetate SLE to extract steroids, MMA, and estrogens from human serum with high recovery.</p><h5><strong>4. Agilent Technologies: Preparing for LC Analysis: What to Consider Ahead of Time</strong></h5><ul><li>We, 24.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6288">Registration</a></li></ul><p>Avoid chromatography problems before they start. Learn how to prepare sample matrices, select and maintain LC columns, and establish an effective system housekeeping list.</p><h5><strong>5. SelectScience: Next-generation metabolomics: From annotation to discovery at scale</strong></h5><ul><li>We, 24.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6281">Registration</a></li></ul><p>Explore how next-generation mass spectrometry, ion mobility, and advanced computational tools accelerate confident small-molecule annotation and discovery in metabolomics.</p><h5><strong>6. Waters Corporation: Clear the Noise: Improving Resolution of GLP-1 Receptor Agonist and Insulin Impurities</strong></h5><ul><li>We, 24.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6262">Registration</a></li></ul><p>Learn how to gain greater resolution for your GLP-1 RA and insulin impurity analysis separations.</p><h5><strong>7. Shimadzu Corporation: Meet LCMS-8065XE: Achieving High-Throughput Ultra-Trace Analysis</strong></h5><ul><li>Th, 25.6.2026 08:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6290">Registration</a></li></ul><p>In this webinar, we will analyze real-world performance data demonstrating how LCMS-8065XE enables high sensitivity analysis even in complex matrices.</p><h5><strong>8. Waters Corporation: Creating Better Chromatographic Methods Using a Systematic Approach</strong></h5><ul><li>Th, 25.6.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6284">Registration</a></li></ul><p>Stop guessing and start separating. Learn a structured, systematic approach to LC method development to improve robustness and accelerate column selection.</p><h5><strong>9. Waters Corporation: Lower the Flow to see even more</strong></h5><ul><li>Th, 25.6.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6285">Registration</a></li></ul><p>Boost large molecule sensitivity in bioanalysis. Learn how switching to microflow LC-MS/MS improves limits of quantification, cuts costs, and reduces solvent usage.</p><h5><strong>10. Separation Science: Advancing PFAS Analysis: From Hidden Precursors to Food Safety Workflows</strong></h5><ul><li>Th, 25.6.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6286">Registration</a></li></ul><p>Discover practical approaches for detecting, analyzing, and addressing PFAS contamination in environmental and food-related samples.</p><h5><strong>11. Waters Corporation: Achieving Accurate BOD Results: What Your Questions Revealed</strong></h5><ul><li>Th, 25.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6283">Registration</a></li></ul><p>Get consistent and defensible BOD results by learning how to troubleshoot common testing errors and standardize your laboratory procedures.</p><h5><strong>12. SelectScience: Future-ready biobanking starts with smarter sample storage</strong></h5><ul><li>Fr, 26.6.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6289">Registration</a></li></ul><p>Safeguard your specimens in biobanking and clinical research. Learn from Thermo Fisher Scientific experts how to optimize sample storage, traceability, and compliance.</p><h5><strong>13. SelectScience: Enhancing Empower software data with cloud-based applications</strong></h5><ul><li>Fr, 26.6.2026 17:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6287">Registration</a></li></ul><p>Connect your chromatography data with Waters’ new cloud-based software solutions. Learn how to track system status in real time and enable AI-powered data analytics.</p>

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Waters SELECT SERIES Cyclic IMS

The SELECT SERIES Cyclic IMS combines novel cyclic ion mobility separation with state-of-the-art, high performance, time-of-flight mass spectrometry.

<div><div id="title-c6692d35e8" data-locator="title-with-text"><h3><strong>Endless possibilities. Limitless research.</strong></h3></div></div><div><div id="text-ba9d3d94da" data-locator="text"><p><strong>Developed through focused collaboration, the SELECT SERIES Cyclic IMS combines novel cyclic ion mobility separation with state-of-the-art, high performance, time-of-flight mass spectrometry, enabling leading researchers to unlock the potential in scientific discovery.</strong></p><p>The SELECT SERIES Cyclic IMS enables selection of ions by both mass and mobility, a unique functionality of <strong>Cyclic Ion Mobility (cIM)</strong> that provides unparalleled flexibility and capabilities for research studies in both academia and industry. With ion mobility and mass spectrometry, you can separate by shape as well as&nbsp;<i>m/z</i>&nbsp;for separation of isomers, measure the <strong>Collisional Cross Section (CCS)</strong> of an ion, and achieve significantly cleaner mass spectral data.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SELECT_SERIES_Cyclic_IMS_Novel_Cyclic_Ion_Mobility_c_IM_Technology_13abf6dd9b.jpg" alt="Waters SELECT SERIES Cyclic IMS: Novel Cyclic Ion Mobility (cIM) Technology." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SELECT_SERIES_Cyclic_IMS_Novel_Cyclic_Ion_Mobility_c_IM_Technology_13abf6dd9b.jpg 234w," sizes="100vw" width="1024" height="682"></p><h3><strong>Overview</strong></h3><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-26eab4b89b" data-locator="text"><ul><li>Combine <strong>cyclic ion mobility (cIM) separation</strong> with <strong>ToF mass spectrometry</strong></li><li>Separate more than ever before with ultimate ion mobility resolution for complete confidence</li><li>Perform <strong>unique IMS<sup>n</sup> experiments</strong> for increased selectivity and advanced characterization</li><li>Enable <strong>pre-mobility mass selection</strong> and <strong>mobility selection of precursor and product ions</strong></li><li>Achieve increased experimental flexibility for solving complex problems with three distinct fragmentation regions</li></ul></div></div><div><div id="text-36ff553b5f" data-locator="text"><h3><strong>Recommended Use</strong></h3><p>For identifying and rapidly monitoring co-eluting, isomeric metabolites in chemical reactions</p><blockquote><p class="cke-user_style-important"><strong>Video: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=nauwVH0L91o"><strong>Waters SELECT SERIES Cyclic IMS</strong></a></p></blockquote><div><div id="text-8469d7776e" data-locator="text"><h3 data-locator="modal-title"><strong>Discover more with ultimate ion mobility resolution</strong></h3><p>The instrument design and control software enable a range of experiments, including acquisition of Tof data, Single pass ion mobility, and the ability to scale mobility resolution to match the complexity of a sample. Ion mobility resolution increases with the length of the mobility device and therefore with the number of passes.</p><figure class="image"><img style="aspect-ratio:1024/682;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SELECT_SERIES_Cyclic_IMS_IMS_resolution_increases_with_the_number_of_passes_around_the_c_IM_device_121719dbe0.jpg" alt="Waters SELECT SERIES Cyclic IMS: IMS resolution increases with the number of passes around the cIM device. Demonstrated here by the separation of reverse sequence peptides SDGRG and GRGDS. The Arrival Time Distributions (ATDs) obtained after 100 passes around the device are inset." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SELECT_SERIES_Cyclic_IMS_IMS_resolution_increases_with_the_number_of_passes_around_the_c_IM_device_121719dbe0.jpg 234w," sizes="100vw" width="1024" height="682"></figure><div><div id="text-bb2fb9f91c" data-locator="text"><h3 data-locator="modal-title"><strong>Unlock the potential in scientific discovery</strong></h3><p>The unique instrument design of the SELECT SERIES Cyclic IMS&nbsp;facilitates advanced ion mobility experiments like IMS<sup>n</sup>&nbsp;isolation. IMS<sup>n</sup>&nbsp;isolation&nbsp;extends the resolution of a specific mobility range,&nbsp;<i>e.g.,</i>&nbsp;ions of a specific species. Eject ions (without acquisition) outside of the desired mobility range, leaving the remaining species in the cIM to continue the separation process. This “trimming” approach can be undertaken numerous times and can be used, for example, to increase specificity of the selected ions or to prevent “wrap-around” in the cIM where high mobility ions catch up with low mobility ions in multi-pass experiments. Multiple cyclic sequences of different numbers of passes can be defined in one, multi-pass LC-MS method, increasing productivity by reducing the need for multiple injections of the same sample.</p></div></div><figure class="image"><img style="aspect-ratio:1024/578;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SELECT_SERIES_Cyclic_IMS_Investigation_into_acyl_glucuronides_via_infusion_f3bf2c67e2.jpg" alt="Waters SELECT SERIES Cyclic IMS: Investigation into acyl glucuronides via infusion. Slicing enables further IMS separation of target metabolite (blue region) of interest and other ions can be ejected. Increasing the IMS resolution reveals an additional 4-O acyl. In collaboration with Imperial College London – Ian D. Wilson." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SELECT_SERIES_Cyclic_IMS_Investigation_into_acyl_glucuronides_via_infusion_f3bf2c67e2.jpg 245w," sizes="100vw" width="1024" height="578"></figure><div><div id="text-78a4dc4d5e" data-locator="text"><h3 data-locator="modal-title"><strong>Undertake advanced structural characterization</strong></h3><p><strong>IMS<sup>n</sup>&nbsp;activation</strong>&nbsp;is ideal for <strong>advanced structural characterization</strong>. During mobility separation, excise a segment of the mobility separated species and send the ions to the pre array storage device. Unwanted ions are ejected, and the stored ions are then re-injected into the array under collision induced activation/dis-sociation conditions, as required, and subsequent mobility separation performed. The product ions can then be sent to the&nbsp;Tof<i>&nbsp;</i>for detection or can undergo further rounds of mobility selection providing the unique IMS<sup>n</sup>&nbsp;capability.</p></div></div><div><div id="text-079d02ceb8" data-locator="text"><h3 data-locator="modal-title"><strong>Imaging mass spectrometry with DESI XS and Cyclic IMS</strong></h3><p>The unique combination of&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=26&amp;mainauthorItems=5&amp;search=DESI+XS"><strong>DESI XS</strong></a><strong>&nbsp;</strong>with SELECT SERIES Cyclic IMS provides ultimate levels of specificity for <strong>MS imaging</strong> approaches. DESI XS produces a visual map of the spatial distribution of a wide range of analytes, for example small molecule drugs, metabolites, and lipids across the surface of a tissue sample, and the unique capabilities and modes of acquisition of Cyclic IMS enable comprehensive in-depth experiments to be performed.</p></div></div><div><div id="text-673d117887" data-locator="text"><h3 data-locator="modal-title"><strong>Increased sensitivity provides confidence in what you find</strong></h3><p>To complement the novel <strong>Cyclic Ion Mobility</strong> capability and for complete confidence in your data, the&nbsp;SELECT SERIES&nbsp;Cyclic IMS is equipped with the latest <strong>ToF technology</strong>. The XS transfer device maintains the fidelity of the ion mobility separation while simultaneously conditioning the ion beam for the absolute best time-of-flight performance. The new offset oa-Tof with dual gain detection system <strong>provides improved sensitivity, resolution &gt;100,000 (FWHM), reliable accurate exact mass measurements</strong> (low ppm), and <strong>improved dynamic range</strong>.</p><figure class="image"><img style="aspect-ratio:1024/682;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mass_spectrum_highlighting_resolution_of_100_000_FWHM_on_the_7_charge_state_of_monomeric_9ff8f53204.jpg" alt="Waters SELECT SERIES Cyclic IMS: Mass spectrum highlighting resolution of >100,000 (FWHM) on the 7+ charge state of monomeric." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mass_spectrum_highlighting_resolution_of_100_000_FWHM_on_the_7_charge_state_of_monomeric_9ff8f53204.jpg 234w," sizes="100vw" width="1024" height="682"></figure></div></div></div></div></div></div></div></div></div></div></div></div>

Waters Cyclic IMS P20 Mass Spectrometer

Cyclic IMS P20 combines high-resolution ion mobility, native MS, and imaging capabilities for detailed structural characterization of complex biological samples.

<p><strong>The Waters Cyclic IMS P20 Mass Spectrometer is a next-generation ion mobility-enabled mass spectrometry platform designed to reveal molecular structure with greater confidence by combining high-resolution cyclic ion mobility separation with accurate mass analysis. The system resolves co-eluting, isomeric, and isobaric species, providing deeper structural insight into complex biological samples.</strong></p><p>Built for advanced research applications, the Cyclic IMS P20 supports proteomics, metabolomics, lipidomics, native mass spectrometry, and mass spectrometry imaging workflows. Multi-pass ion mobility separations, Wideband Enhancement (WBE), and advanced fragmentation technologies provide exceptional analytical depth and sensitivity.</p><p>The platform supports <strong>MALDI XS and DESI XS imaging</strong> workflows, enabling high-confidence spatial analysis and molecular visualization. Additional capabilities including CIU, HDX, ECD, and SID make the system ideally suited for investigating biomolecular structure, stability, dynamics, and intermolecular interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Cyclic_IMS_P20_Mass_Spectrometer_with_MALDI_XS_and_DESI_XS_1a6ac479a1.jpg" alt="Waters: Cyclic IMS P20 Mass Spectrometer with MALDI XS and DESI XS" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Cyclic_IMS_P20_Mass_Spectrometer_with_MALDI_XS_and_DESI_XS_1a6ac479a1.jpg 234w," sizes="100vw" width="1024" height="682"></p><h3><strong>Key Features</strong></h3><ul><li>High-resolution Cyclic Ion Mobility Spectrometry</li><li>Separation of isomeric, isobaric, and co-eluting species</li><li>Multi-pass ion mobility separation</li><li>Wideband Enhancement (WBE) technology</li><li>Up to 10× improvement in MS/MS sensitivity</li><li>Advanced native MS capabilities</li><li>Dynamic Field Declustering (DFD)</li><li>Quadrupole mass selection up to 32,000 m/z</li><li>Extended mass range beyond 100,000 m/z</li><li>Electron Capture Dissociation (ECD)</li><li>Surface-Induced Dissociation (SID)</li><li>Integrated Collision-Induced Unfolding (CIU)</li><li>Hydrogen-Deuterium Exchange (HDX) workflows</li><li>MALDI XS and DESI XS imaging support</li><li>Automated CCS calibration</li><li>Intuitive software environment</li><li>Modular and future-proof platform</li></ul><h3><strong>Technical Specifications</strong></h3><h4><strong>Technology</strong></h4><ul><li>Cyclic Ion Mobility Spectrometry (Cyclic IMS)</li><li>Orthogonal Acceleration Time-of-Flight (oaTOF)</li><li>Multi-pass ion mobility separation</li><li>IMSⁿ experiments</li></ul><h4><strong>Advanced Capabilities</strong></h4><ul><li>Wideband Enhancement (WBE)</li><li>Dynamic Field Declustering (DFD)</li><li>Collision-Induced Unfolding (CIU)</li><li>Electron Capture Dissociation (ECD)</li><li>Surface-Induced Dissociation (SID)</li><li>Hydrogen-Deuterium Exchange (HDX)</li></ul><h4><strong>Mass Range</strong></h4><ul><li>Quadrupole selection up to 32,000 m/z</li><li>Detection beyond 100,000 m/z</li></ul><h4><strong>Imaging Applications</strong></h4><ul><li>MALDI XS</li><li>DESI XS</li><li>High-resolution molecular imaging</li><li>Isomeric lipid separation</li></ul><h4><strong>Software</strong></h4><ul><li>Automated CCS calibration</li><li>Advanced method development</li><li>IMS data processing and interpretation</li><li>HDX and CIU workflow support</li></ul><h4><strong>Typical Applications</strong></h4><ul><li>Proteomics</li><li>Native mass spectrometry</li><li>Structural biology</li><li>Lipidomics</li><li>Metabolomics</li><li>Biotherapeutic characterization</li><li>GLP-1 research</li><li>Protein complex analysis</li><li>Molecular imaging</li><li>Forensics</li><li>Environmental analysis</li></ul>

Waters SYNAPT XS High Resolution Mass Spectrometer

The SYNAPT XS Ion Mobility Time-of-Flight Mass Spectrometer provides ultimate flexibility, offering greater freedom of analytical choice to support scientific creativity and technical success for any application.

<div><div><h4><strong>Ultimate flexibility for deeper discovery</strong></h4></div></div><div><div><p><strong>In the arena of scientific discovery, the slow pace of advancement and escalating costs of research can prove to be a challenge. Unlike competitor systems with restricted inlet options, scan function limitations, or requiring multiple platforms, only Waters offers an all-encompassing high-performing LC-MS solution, the SYNAPT XS mass spectrometer, for ultimate flexibility and greater freedom of analytical choice – supporting scientific creativity and technical success for any application.</strong></p><ul><li><strong>Novel technology building blocks deliver cutting-edge analytical performance</strong></li><li><strong>SONAR</strong> and <strong>HDMSE</strong> provide a unique toolkit for interrogating complex mixtures</li><li><strong>Ion mobility</strong> multiplies peak capacity and selectivity of analysis</li><li><strong>CCS measurements</strong> increase security of compound identification</li><li><strong>Deeper discovery in Mass Spectrometry Imaging</strong> with<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/Full-Spectrum-Molecular-Imaging-/nav.htm?cid=135070661"><strong>Full Spectrum Molecular Imaging</strong></a> enabled by <a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/DESI-XS---Desorption-Electrospray-Ionization-/nav.htm?cid=135047466"><strong>DESI XS</strong></a> and MALDI</li></ul><h4><strong>Overview</strong></h4><div><div><div><div><ul><li><strong>Serve the broadest range of applications </strong>with the most extensive range of chromatograph inlets for ultimate flexibility</li><li><strong>Attain fragment ion information for every detectable component</strong> for unambiguous confirmation of compound identity with DIA modes <strong>SONAR</strong>, MSE, and <strong>HDMSE</strong></li><li><strong>Generate rich fragmentation data</strong> from highly complex samples in all acquisition modes including DDA</li><li>Simplify and streamline the mass spectral imaging workflow and deliver multi-layered, information-rich data with multiple imaging techniques and accompanying High Definition Imaging (HDI) Software</li><li><strong>Achieve automated higher order structure determination </strong>thorough confident identification, intact mass analysis, and best-in-class reproducibility</li><li><strong>Enhance MS/MS possibilities</strong> using complementary fragmentation techniques with high resolution and accurate mass measurements</li></ul></div></div><div><div><h5><strong>Recommended Use</strong></h5><p>For deeper discovery by aligning complimentary workflows on a single <strong>Ion mobility</strong> enabled time-of-flight mass spectrometer with the flexibility of multiple ion sources and acquisition modes.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_4_696d77ce2b.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: The Waters SYNAPT XS offers flexibility and freedom of analytical choice with an extensive range of chromatographic inlets." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_4_696d77ce2b.jpg 234w," sizes="100vw" width="1024" height="682"></p><div><div><h3><strong>Cutting edge performance with novel technology</strong></h3><p>With technology building blocks inherited from the <strong>Waters Advanced Mass Spectrometry</strong> <strong>SELECT SERIES</strong>, advanced innovation is built-in, ensuring scientists using this platform are at the cutting edge of mass spectrometry while retaining the ease of use and established end-to-end workflows for which the <strong>SYNAPT </strong>is well known.</p><ul><li><strong>StepWave&nbsp;XS -&nbsp;</strong>Redesigned segmented quadrupole transfer optics provide enhanced sensitivity for challenging compounds while further improving levels of analytical robustness.</li><li><strong>Extended ToF -&nbsp;</strong>Delivers UPLC-compatible mass resolution, matrix-tolerant dynamic range, and quantitative results for the most complex samples, while offering superior specifications to previous SYNAPT models.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_9f19abb382.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: SYNAPT XS schematic." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_9f19abb382.jpg 245w," sizes="100vw" width="1024" height="546"></p></div></div><div><div><h3><strong>Interrogate complex mixtures with a unique toolkit</strong></h3><p>A complete analytical strategy requires the combination of appropriate complementary techniques to see the bigger picture. With both <strong>SONAR</strong> and IMS-based <strong>data independent acquisition</strong> (DIA) modes of operation on the SYNAPT XS, the discovery analyst is in a unique position to interrogate complex mixtures by complementary mechanisms. Both types of acquisition increase analytical <strong>peak capacity</strong>, providing ‘clean and clear’ fragmentation data, but based on different molecular properties. This provides a truly unique investigative toolbox for the in-depth interrogation of complex mixtures.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_2_9179e575a4.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: During each scan the quadrupole separates co-eluting precursor masses by scanning the mass range, and transiting them in sequence. Fragment ions from different precursors can then be recorded separately and assigned with confidence." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_2_9179e575a4.jpg 245w," sizes="100vw" width="1024" height="593"></p></div></div><div><div><h3><strong>Discover more with ion mobility and CSS measurement</strong></h3><p>Conventional mass spectrometers separate components based on m/z. The SYNAPT XS also allows separation of molecules according to<strong> their size, shape, and charge during ion mobility experiments as a function of their collisional cross section</strong> (CCS). As well as ion mobility offering an additional dimension of resolution, multiplying <strong>peak capacity</strong>, and <strong>selectivity of analysis</strong> the measurement of CCS offers additional molecular identifiers. The measured CCS of an ion can be used to help confirm an ion’s identity or investigate its structure. The inclusion of ion mobility significantly increases the extent and confidence with which a scientist can profile complex mixtures and complex molecules – dramatically enhancing sample definition.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_3_2240bff527.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: Retention and drift time principle ion mobility enabled data-independent LC-HDMSE analysis (LC-DIA-IM-MS)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_3_2240bff527.jpg 204w," sizes="100vw" width="1024" height="784"></p></div></div><div><div><h3><strong>Achieve ultra-confident structural characterization of components</strong></h3><p>Time aligned parallel (TAP) fragmentation is an acquisition mode unique to the T-Wave IMS design. It permits users to take the advantage of the <strong>TriWave configuration</strong>, which allows the pre-IMS T-Wave and post-IMS T-Wave to operate as two separate collision cells. The resultant CID-IMS-CID instrumental operation helps to provide ultra-confident structural characterization of components. <strong>TAP fragmentation</strong> provides a distinct advantage for building a complete structure, through superior fragment ion coverage, sensitivity, and accuracy compared to traditional MSⁿ or MS/MS techniques.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_5_4dd33f1ab5.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: Schematic showing Time Aligned Parallel Fragmentation. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_5_4dd33f1ab5.jpg 234w," sizes="100vw" width="1024" height="682"></p></div></div></div></div></div></div></div></div>

Products from category Mass detectors focused on Ion Mobility from Waters - page 1

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