Waters SELECT SERIES Cyclic IMS

Endless possibilities. Limitless research.

Developed through focused collaboration, the SELECT SERIES Cyclic IMS combines novel cyclic ion mobility separation with state-of-the-art, high performance, time-of-flight mass spectrometry, enabling leading researchers to unlock the potential in scientific discovery.

The SELECT SERIES Cyclic IMS enables selection of ions by both mass and mobility, a unique functionality of Cyclic Ion Mobility (cIM) that provides unparalleled flexibility and capabilities for research studies in both academia and industry. With ion mobility and mass spectrometry, you can separate by shape as well as m/z for separation of isomers, measure the Collisional Cross Section (CCS) of an ion, and achieve significantly cleaner mass spectral data.

Waters SELECT SERIES Cyclic IMS: Novel Cyclic Ion Mobility (cIM) Technology.

Overview

• Combine cyclic ion mobility (cIM) separation with ToF mass spectrometry
• Separate more than ever before with ultimate ion mobility resolution for complete confidence
• Perform unique IMSⁿ experiments for increased selectivity and advanced characterization
• Enable pre-mobility mass selection and mobility selection of precursor and product ions
• Achieve increased experimental flexibility for solving complex problems with three distinct fragmentation regions

Recommended Use

For identifying and rapidly monitoring co-eluting, isomeric metabolites in chemical reactions

Video: Waters SELECT SERIES Cyclic IMS

Discover more with ultimate ion mobility resolution

The instrument design and control software enable a range of experiments, including acquisition of Tof data, Single pass ion mobility, and the ability to scale mobility resolution to match the complexity of a sample. Ion mobility resolution increases with the length of the mobility device and therefore with the number of passes.

Waters SELECT SERIES Cyclic IMS: IMS resolution increases with the number of passes around the cIM device. Demonstrated here by the separation of reverse sequence peptides SDGRG and GRGDS. The Arrival Time Distributions (ATDs) obtained after 100 passes around the device are inset.

Unlock the potential in scientific discovery

The unique instrument design of the SELECT SERIES Cyclic IMS facilitates advanced ion mobility experiments like IMSⁿ isolation. IMSⁿ isolation extends the resolution of a specific mobility range, e.g., ions of a specific species. Eject ions (without acquisition) outside of the desired mobility range, leaving the remaining species in the cIM to continue the separation process. This “trimming” approach can be undertaken numerous times and can be used, for example, to increase specificity of the selected ions or to prevent “wrap-around” in the cIM where high mobility ions catch up with low mobility ions in multi-pass experiments. Multiple cyclic sequences of different numbers of passes can be defined in one, multi-pass LC-MS method, increasing productivity by reducing the need for multiple injections of the same sample.

Waters SELECT SERIES Cyclic IMS: Investigation into acyl glucuronides via infusion. Slicing enables further IMS separation of target metabolite (blue region) of interest and other ions can be ejected. Increasing the IMS resolution reveals an additional 4-O acyl. In collaboration with Imperial College London – Ian D. Wilson.

Undertake advanced structural characterization

IMSⁿ activation is ideal for advanced structural characterization. During mobility separation, excise a segment of the mobility separated species and send the ions to the pre array storage device. Unwanted ions are ejected, and the stored ions are then re-injected into the array under collision induced activation/dis-sociation conditions, as required, and subsequent mobility separation performed. The product ions can then be sent to the Tof for detection or can undergo further rounds of mobility selection providing the unique IMSⁿ capability.

Imaging mass spectrometry with DESI XS and Cyclic IMS

The unique combination of DESI XS with SELECT SERIES Cyclic IMS provides ultimate levels of specificity for MS imaging approaches. DESI XS produces a visual map of the spatial distribution of a wide range of analytes, for example small molecule drugs, metabolites, and lipids across the surface of a tissue sample, and the unique capabilities and modes of acquisition of Cyclic IMS enable comprehensive in-depth experiments to be performed.

Increased sensitivity provides confidence in what you find

To complement the novel Cyclic Ion Mobility capability and for complete confidence in your data, the SELECT SERIES Cyclic IMS is equipped with the latest ToF technology. The XS transfer device maintains the fidelity of the ion mobility separation while simultaneously conditioning the ion beam for the absolute best time-of-flight performance. The new offset oa-Tof with dual gain detection system provides improved sensitivity, resolution >100,000 (FWHM), reliable accurate exact mass measurements (low ppm), and improved dynamic range.

Waters SELECT SERIES Cyclic IMS: Mass spectrum highlighting resolution of >100,000 (FWHM) on the 7+ charge state of monomeric.