This workflow confirms and quantifies 31 drugs of abuse in oral fluid (including THC) in a 4.5‑minute LC run on the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149">Thermo Scientific Stellar mass spectrometer</a> using full scan + targeted MS2 (tMS2) with both HCD and CID fragmentation. With 1 µL injection and 0.1% formic acid in water/methanol mobile phases, the method supports ~13 samples/hour (up to ~320/day continuous operation) while maintaining confirmation performance via ion ratios.<blockquote><h5>Method at a glance</h5><ul><li>What matrix is tested? Oral fluid</li><li>How many drugs? 31 drugs of abuse (panel includes THC)</li><li>Run time? 4.5 minutes</li><li>Throughput? ~13 samples/hour → up to ~320 samples/day (24‑hour operation)</li><li>Injection volume? 1 µL</li><li>Mobile phases? 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B)</li><li>MS acquisition? Full scan + targeted MS2 (tMS2)</li><li>Fragmentation? Compound‑optimized HCD and CID in the same targeted list</li><li>Confirmation strategy? Ion ratios with ±20% tolerance (typical confirmatory approach)</li></ul></blockquote><h3>Why oral fluid—and why speed matters</h3>Oral fluid is attractive for clinical and forensic toxicology because collection is simple and the matrix aligns well with high‑volume workflows (workplace/occupational, roadside, and other settings). The challenge is building a method that’s simultaneously:<ul><li>Broad enough for today’s drug panels</li><li>Sensitive enough to reach required cutoffs</li><li>Fast enough to keep sample queues under control</li><li>Confirmation‑ready (consistent product ions and ion ratios)</li></ul>A 4.5‑minute method is a practical lever here: compress runtime, raise throughput, and still keep confirmation quality by optimizing MS/MS conditions per compound.<h3>The LC method</h3>A short, efficient separation is the foundation of the workflow:<ul><li>UHPLC system: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Thermo Scientific Vanquish Horizon UHPLC</a></li><li>Column: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/424">Thermo Scientific Accucore Biphenyl</a>, 50 × 2.1 mm, 2.6 µm</li><li>Mobile phase A: 0.1% formic acid in water</li><li>Mobile phase B: 0.1% formic acid in methanol</li><li>Injection volume: 1 µL</li><li>Gradient runtime: 4.5 minutes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_fd3f3e8ea4.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 1: Thermo Scientific Vanquish Horizon UHPLC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_fd3f3e8ea4.jpg 120w," sizes="100vw" width="747" height="969"><h3>The MS method (Full scan + targeted MS2 with HCD + CID)</h3><h5>Acquisition approach</h5>This method uses a “two‑layer” strategy for quantitation + confirmation:<ol><li>Full scan (broad coverage, context, quantitative support)</li><li>Targeted MS2 (tMS2) (confirmation‑focused product ions, ion ratios)</li></ol><h5>The key differentiator: HCD and CID in one targeted list</h5>Instead of forcing every analyte into a single fragmentation style, the method uses both:<ul><li>HCD (beam‑type fragmentation)</li><li>CID (resonance‑type fragmentation)</li></ul><h5>Why that improves method development:</h5>Drugs of abuse span multiple chemistries. A single collision approach often works “okay” for most compounds—but one or two analytes can become persistent problems (weak qualifiers, unstable ion ratios, excess noise). Having both HCD and CID available in one method lets you optimize per compound and move faster.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_3_e4a95651a2.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 2: Thermo Scientific Stellar mass spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_3_e4a95651a2.jpg 245w," sizes="100vw" width="645" height="377"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_5_88276eec6a.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 3: Full scan &amp; targeted MS2 methods, HCD and collision energies used." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_5_88276eec6a.jpg 245w," sizes="100vw" width="768" height="279"><h3>Why CID + HCD flexibility improves confirmation</h3>In this workflow, HCD performed well for most compounds. But for buprenorphine, HCD produced one dominant product ion with additional low m/z noise and noticeable residual precursor. Using CID generated two high‑intensity product ions and delivered consistent ion ratios across the calibration range.When your confirmation hinges on stable ion ratios, the ability to switch fragmentation mode (not just collision energy) is a method‑development advantage—especially for “problem analytes.”<h3>Controllable ionization</h3>Oral fluid methods often deal with limited sample volume and matrix effects. This workflow pairs low injection volume with ionization control using the Thermo Scientific OptaMax Plus HESI source.<ul><li>The OptaMax Plus HESI source supports high probe temperature capability (up to 650 °C).</li><li>In this study, a vaporizer temperature of 500 °C was used to help achieve sensitivity and improve relative peak areas across drugs of abuse.</li></ul>Why this matters: If you’re injecting only 1 µL, ionization stability and efficiency can be the difference between “meets cutoff” and “misses at LOQ.”<h3>Sample preparation</h3>Oral fluid panels become more challenging when THC is included because most drugs of abuse are basic, while THC is largely neutral, and that can stress extraction chemistry.In this workflow, oral fluid calibrators and samples were extracted using a mixed‑mode approach designed to recover THC alongside other drugs:<ul><li>Calibrators prepared across eight levels (broad dynamic range)</li><li>Extraction performed using mixed‑mode chemistry tips (SCX/WAX) in a dispersive SPE‑style workflow</li><li>Steps automated on a liquid handling platform to support high‑throughput consistency</li><li>Samples dried and reconstituted prior to LC‑MS analysis</li><li>Only 1 µL injected per run</li></ul>Even if your lab uses different automation or consumables, the method structure (fast LC + full scan + targeted MS2 with dual fragmentation) transfers well.<blockquote><h5>Throughput math</h5><ul><li>4.5 minutes per injection → 60 / 4.5 = 13.3 injections/hour</li><li>That’s ~13 samples/hour in typical phrasing</li><li>Over 24 hours: 13.3 × 24 = ~320 samples/day (continuous operation)</li></ul>If you run a single shift, just scale accordingly (e.g., ~106/day for an 8‑hour shift, excluding QC and overhead).</blockquote><h3>Performance summary</h3><h5>Sensitivity and linearity</h5><ul><li>Linearity from LOQs as low as 0.25 ng/mL up to 1,000 ng/mL (panel-dependent)</li><li>For THC specifically: LOQ at 1 ng/mL, with a dynamic range extending to 1,000 ng/mL in this evaluation</li><li>Across the panel, LOQs were reported as lower than recommended confirmation cutoffs referenced in SAMHSA guidance and NSC Tier 1 cutoffs (study-specific outcome)</li></ul><h5>Confirmation via ion ratios</h5><ul><li>Ion ratios evaluated using ±20% tolerance (typical confirmatory acceptance framework)</li><li>The workflow produced consistent ion ratios across calibrator levels for the full panel in this evaluation</li></ul><h5>Case sample comparison (real-world relevance)</h5>When compared against a validated triple quadrupole method for case study samples, the Stellar MS workflow:<ul><li>Confirmed the positive hits observed by the reference method</li><li>Reported a few additional positives for select analytes, attributed to lower LOQs</li><li>Highlighted an operational efficiency point: 1 µL injection in this method versus 20 µL injection in the compared method</li></ul><h3>FAQ</h3><div><div><ol><li>How long does the LC‑MS run take for the 31‑drug oral fluid panel? 4.5 minutes per injection using a short UHPLC gradient. &nbsp;</li><li>How many drugs of abuse can Stellar MS detect in this workflow? 31 drugs of abuse, including THC, using full scan + targeted MS2. &nbsp;</li><li>What MS/MS approach is used? A combined strategy: full scan plus targeted MS2 (tMS2) for confirmation. &nbsp;</li><li>Why use both HCD and CID? Because some analytes fragment better—and produce more stable confirmation ions/ion ratios—under one mode versus the other. Using both increases flexibility and speeds method optimization. &nbsp;</li><li>What injection volume is used? 1 µL of extracted sample. &nbsp;</li><li>What mobile phases are used? 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B). &nbsp;</li><li>How many samples can you run per day? At 4.5 minutes/injection, throughput is ~13 samples/hour or up to ~320/day with continuous operation (actual throughput depends on QC and scheduling).</li></ol></div></div><h3>What makes this workflow “easy” to develop on Stellar MS</h3>Here’s the short list of features that directly reduce method‑development friction:<ul><li>HCD or CID in one method → optimize per compound, not compromise</li><li>Fast MS2 acquisition speeds (up to 140 Hz for MS2) → more data points across narrow peaks</li><li>Fast polarity switching (true 5 ms) → supports mixed panels efficiently</li><li>Adaptive RT (real‑time retention time window adjustment) → helps reduce reinjections when retention times drift (e.g., column aging) and supports method transfer from longer to shorter gradients</li><li>OptaMax Plus HESI source control → supports sensitivity at low injection volumes</li></ul>If you’re building a fast oral fluid confirmation workflow—or updating an existing DOA panel—this 4.5‑minute method shows how Stellar MS can combine speed, confirmation-ready MS2, and fragmentation flexibility (HCD + CID) in a single targeted workflow. Reach out to learn how to adapt the targeted list, collision settings, and scheduling strategy to your lab’s cutoffs and throughput goals.<blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#MassSpectrometry #OralFluid #DrugsOfAbuse #MethodDevelopment #HighThroughput</blockquote>

4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS

<h2><a target="_blank" rel="noopener noreferrer" href="https://www.isc2026.org/">ISC 2026</a></h2>The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/314">International Symposium on Chromatography (ISC)</a> represents the oldest conference series on separation science. ISC symposia have been organised since 1956 in each even year. ISC is one of the premier meetings series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/644">35th International Symposium on Chromatography</a></h4>📆6. - 10. September 2026📍Prague, Czech Republic<h3>REGISTRATION</h3><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://www.czech-in.org/cmPortalV15/Account/Login?ReturnUrl=%2FcmPortalV15%2FPortal%2FISC26%2Fnormal">Register HERE for the ISC 2026</a>Late Registration until 25 August 2026</blockquote><h3>INDUSTRY ACTIVITIES</h3><h4>Activity 1: Discussion Session: Industry &amp; Education</h4><ul><li>Monday, 7 September 2026 | 16:30-17:50</li><li>Place: Club A</li><li>Moderators: Tony Edge and Deirdre Cabooter&nbsp;</li></ul><h5>Concept:</h5>This discussion session will explore the relationship between education in separation science in academia and the expectations of industry, focusing on how universities and companies can better collaborate to prepare graduates for successful careers in analytical laboratories and R&amp;D environments. The goal of the session is to provide perspectives from both academia and industry on how education in chromatography and related separation techniques can evolve to better reflect current technological, methodological, and regulatory challenges.<h5>Discussion Topics:</h5><ul><li>Bridging academia and industry: how both sectors perceive current cooperation and where improvements are possible&nbsp;</li><li>Graduate preparedness: which skills and competencies are often missing when entering industrial roles, and in which areas graduates typically excel&nbsp;</li><li>Approaches to teaching separation science: how chromatography and related analytical techniques are taught across different universities and regions&nbsp;</li><li>Industry support for education: examples of collaboration such as internships, industrial placements, joint teaching activities, or access to instrumentation&nbsp;</li><li>Experiences with academia–industry partnerships: what models of cooperation have proven effective in practice&nbsp;</li><li>Future perspectives: how education in separation science should adapt to emerging trends such as automation, digitalization, sustainability, and regulatory requirements&nbsp;</li></ul><h5>Format:</h5>Moderated panel discussion with short introductory remarks from invited speakers representing both academia and industry, followed by an interactive discussion with the audience.<h5>Panelists:</h5><ul><li>Shimadzu&nbsp;</li><li>Novartis&nbsp;</li><li>Jelle De Vos, RIC Group&nbsp;</li><li>Davy Guillarme, University of Geneva&nbsp;</li><li>Caterina Temporini, University of Pavia&nbsp;</li></ul><h4>Activity 2: Career Presentation&nbsp;</h4><ul><li>Monday, 7 September 2026 | 18:00-19:00</li><li>Place: Club A</li><li>Moderators: Adrian Clarke and Martina Catani</li></ul><h5>Concept:</h5>This session will provide an overview of career opportunities in separation science across industry, highlighting the wide range of professional pathways available to graduates and experienced scientists working in chromatography and related analytical disciplines.Participating companies will introduce their organizations, present typical roles involving separation techniques, and share insights into the skills and competencies they seek in candidates. The session is intended to support students, early-career researchers, and experienced professionals interested in exploring career options in industry sectors.<h5>Discussion Topics:&nbsp;</h5><ul><li>Overview of career paths involving chromatography and separation science&nbsp;</li><li>Entry-level opportunities for graduates and PhD holders&nbsp;</li><li>Positions for experienced scientists and technical specialists&nbsp;</li><li>Roles in R&amp;D, analytical development, quality control, regulatory science, application support, and instrument development&nbsp;</li><li>Skills and competencies valued by employers in separation science&nbsp;</li><li>Internships, graduate programs, and early-career development opportunities&nbsp;</li><li>Mobility between academia and industry&nbsp;</li></ul><h5>Format:</h5>Short presentation providing:<ul><li>overview of different carrier paths&nbsp;</li><li>introduction to the company and its activities related to separation science&nbsp;</li><li>examples of typical roles and career progression paths&nbsp;</li><li>information about current or expected hiring needs&nbsp;</li><li>advice for candidates interested in pursuing careers in industry&nbsp;</li><li>the session should include time for informal discussion, allowing participants to interact with company representatives and learn more about specific opportunities.&nbsp;</li></ul><h4>Activity 3: Job Fair</h4><ul><li>Monday, 7 September and Tuesday, 8 September 2026 | 12:30-14:00</li><li>Place: Club A</li><li>Main organizer: Adrian Clarke</li></ul><h5>Concept:</h5>The ISC 2026 Job Fair provides a structured opportunity for direct interaction between companies active in separation science and conference participants seeking career opportunities. The session is designed to facilitate efficient, focused conversations between employers and candidates interested in positions related to chromatography and analytical science.Participating candidates will submit their CV in advance allowing companies to review profiles prior to the event and identify potential matches based on expertise, experience, and career interests.<h5>Format:</h5>The Job Fair will follow a structured rotation format:<ul><li>Each participating company will be assigned a dedicated table&nbsp;</li><li>One company representative will meet candidates individually&nbsp;</li><li>Each candidate will have 5 minutes per meeting to introduce their background and interests&nbsp;</li><li>Participants will rotate between companies at timed intervals&nbsp;</li><li>Session timing will be managed by the organizer, with a signal indicating when to move to the next meeting&nbsp;</li></ul><h5>Candidates:</h5>Participants interested in joining the Job Fair are asked to:<ul><li>submit their CV before August 23rd &nbsp;</li><li>indicate areas of expertise and career interests&nbsp;</li><li>specify whether they are seeking internships, entry-level roles, or experienced positions&nbsp;</li><li>specify if you cannot be available on a certain day e.g. owing to poster presentation&nbsp;</li><li>send these materials to: <a href="mailto:careers@isc2026.org">careers@isc2026.org</a>&nbsp;</li></ul><h5>Companies:</h5>Confirmed participating companies to date include:<ul><li>Novartis&nbsp;</li><li>RIC Group&nbsp;</li><li>Shimadzu&nbsp;</li><li>further companies to be added&nbsp;</li></ul>Potential employers please register your interest to <a href="mailto:careers@isc2026.org">careers@isc2026.org</a> to participate in the speed-dating (first come first served basis).“Jobs position” board will be available in the Careers fair in the foyer in the symposium venue and attendees can visit the company booths in the exhibition hall or discuss with recruiting companies during the conference.<blockquote>Recruiters please send an email with your open positions to <a href="careers@isc2026.org">careers@isc2026.org</a> by the 23rd August and bring the job adverts to post on the jobs board (there will also be the possibility to upload the open positions to the Symposium app).</blockquote><h4>Activity 4: Discussion Session: Artificial Intelligence in Separation Science: From Concept to Practice</h4><ul><li>Tuesday, 8 September 2026 | 16:30-18:00</li><li>Place: Club A</li><li>Moderators: Bob Pirok</li></ul><h5>Concept:</h5>Artificial intelligence (AI) and data-driven approaches are increasingly influencing analytical chemistry, offering new possibilities for method development, data interpretation, process optimization, and decision support. This discussion session will explore how AI is currently being applied in separation science, with a particular focus on practical implementation in industry environments.The session aims to provide a realistic overview of the current state of AI adoption, highlighting both successful applications and remaining challenges. Contributions from industry and academia will help clarify where AI already delivers value, where expectations may exceed current capabilities, and what developments are needed to enable broader implementation.<h5>Discussion Topics:&nbsp;</h5><ul><li>Current applications of AI and machine learning in separation science&nbsp;</li><li>Use of AI in chromatographic method development and optimization&nbsp;</li><li>Data analysis, peak detection, and automated interpretation of complex datasets&nbsp;</li><li>AI-supported troubleshooting and predictive maintenance of analytical instruments&nbsp;</li><li>Integration of AI into regulated environments (e.g., pharmaceutical industry)&nbsp;</li><li>Data requirements, data quality, and standardization challenges&nbsp;</li><li>Comparison between academic developments and real-world industrial implementation&nbsp;</li><li>Barriers to adoption, including regulatory considerations, validation requirements, and transparency of models&nbsp;</li><li>Future outlook: digital laboratories, autonomous method development, and AI-assisted decision-making in analytical workflows&nbsp;</li></ul><h5>Format:</h5>Moderated panel discussion with short introductory remarks from invited speakers representing both academia and industry, followed by an interactive discussion with the audience.<h4>Activity 5: Discussion Session: Sustainability in Separation Science</h4><ul><li>Wednesday, 9 September 2026 | 16:30-18:00</li><li>Place: Club A</li><li>Moderators: Gertrud Morlock</li></ul><h5>Concept:</h5>Sustainability has become an increasingly important priority in analytical science, driving the development of greener methods, reduced solvent consumption, improved energy efficiency, and more responsible laboratory practices. While academic research frequently proposes innovative sustainable approaches, their implementation in industry is often influenced by additional considerations such as robustness, regulatory compliance, cost efficiency, scalability, and long-term reliability.This discussion session aims to explore how sustainability principles are applied in real industrial environments and how collaboration between academia and industry can help translate promising research concepts into practical, validated solutions.By bringing together perspectives from both sectors, the session will highlight opportunities to better align research priorities with industrial needs and identify realistic pathways toward more sustainable separation workflows.<h5>Discussion Topics:&nbsp;</h5><ul><li>Reduction of solvent consumption and the transition to more environmentally friendly mobile phases&nbsp;</li><li>Miniaturized and more efficient separation and extraction techniques&nbsp;</li><li>Energy consumption and efficiency of laboratory processes and analytical instrumentation&nbsp;</li><li>Lifecycle considerations for columns, consumables, and laboratory waste&nbsp;</li><li>Metrics and approaches for measuring and evaluating the “greenness” of analytical methods&nbsp;</li><li>Regulatory constraints and requirements that may limit the implementation of greener analytical workflows&nbsp;</li></ul><h5>Format:</h5>Moderated panel discussion with short introductory contributions from invited speakers representing industry, academia, and technology providers, followed by interactive discussion with the audience.<h5>Panelists:</h5><ul><li>Sébastien Rolle, Shimadzu&nbsp;</li><li>Ken Cook, Thermo Fisher Scientific&nbsp;</li><li>Andreas Otto, Agilent&nbsp;</li><li>Janusz Pawliszyn, University of Waterloo&nbsp;</li><li>Giorgia Purcaro, University of Liège&nbsp;</li></ul>

Industry program at ISC 2026

We are delighted to invite food scientists from academia and industry, representatives of (inter)national agencies, control authorities, governmental and commercial laboratories to attend <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/928">RAFA 2026</a> (12th Symposium on Recent Advances in Food Analysis), that takes place 3–6 November 2026 in Prague..<blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://rafa.easyevent.eu/tickets/">SUBMIT ABSTRACT</a> FOR POSTER PRESENTATION by August 31, 2026Do not miss the opportunity to present your latest results at RAFA 2026.</blockquote><h5>Next deadlines:</h5><ul><li>August 31, 2026: 2nd window – &nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/sponsors_exhibitors_media_partners_info.html">Application for participation as a Sponsor or Exhibitor</a></li><li>September 30, 2026:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/registration.html">Registration at the regular rate</a></li><li>Abstract submission – Last minute posters (May not appear in the RAFA 2026 program)</li></ul></li></ul>Since its first edition in 2003, RAFA has grown into a prestigious international event, typically welcoming up to 850 participants from more than 60 countries worldwide, with the support of more than 50 sponsors, exhibitors, media and supporting partners.RAFA 2026 will provide an overview of contemporary trends in the ANALYTICAL &amp; BIOANALYTICAL STRATEGIES IN FOOD QUALITY AND SAFETY CONTROL and discuss the CHALLENGES AND NOVEL APPROACHES IN FOOD AND NATURAL PRODUCTS ANALYSIS.View the <a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/invited_speakers.html">list of distinguished speakers</a> who have accepted our invitation to RAFA 2026 and will present current hot topics and future perspectives in food analysis!<blockquote>👉More details on the <a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/scientific_programme.html">RAFA 2026 preliminary program</a> can be found HERE.👉OUR <a target="_blank" rel="noopener noreferrer" href="https://www.rafa2026.eu/about_sponsors_exhibitors_partners.html">SPONSORS, EXHIBITORS, MEDIA and SUPPORTING PARTNERS</a></blockquote>You are kindly invited to join and contribute to our exciting scientific program and unforgettable social events!We look forward to meeting you again in Prague!<ul><li>Prof. Jana Pulkrabova &amp; Dr. Stefan van Leeuwen, Symposium Chairs</li><li>Prof. Michele Suman, Symposium Co-chair</li><li>Prof. Jana Hajslova &amp; Prof. Michel Nielen, Symposium Honorary Chairs</li></ul>

RAFA 2026 - Deadline for Abstract Submission for poster presentation is coming

Article

Product

Events

Application

Food

Video

RAFA provides an overview of contemporary trends in ANALYTICAL & BIOANALYTICAL STRATEGIES IN FOOD QUALITY AND SAFETY CONTROL and discusses CHALLENGES / NOVEL APPROACHES IN FOOD AND NATURAL PRODUCTS ANALYSIS.

Recent Advances in Food Analysis (RAFA)

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

ECOM PREPSYSTEM - Chromatography Separation System

A complete flash/preparative/pilot scale/production modular system ready for customizable to the application needs.

We would like to introduce an ECOM product news – a complete flash/preparative/pilot scale/production modular system with an excellent price/performance ratio.<h3>Applications</h3>The system can be used e.g. in:<ul><li>high-capacity systems or with centrifugal liquid/liquid chromatography systems, like in pharmaceutical purification of plant extracts – e.g. cannabinoids (CBD, CBN, CBG, CBC, THC, etc.), peptides, and oligonucleotides, algae extracts, oleoresins, etc.,&nbsp;</li><li>Also in new molecule development or synthesis.</li></ul><h3>Main parts</h3><h4>Pumps</h4><ul><li>The device has 3 pumps, 2 main ones are intended for a "high" pressure gradient (parallel pump connection), and one of them has a 6-solvent selection valve.&nbsp;</li><li>An optional third pump is used for repetitive sampling of high-volume samples. The unit also has a loop sample injection valve and a switching valve for CCC/CPC applications (ascending/descending mode).&nbsp;</li><li>The unit's universal PDA detector measures absorbance at four wavelengths at the same time up to 800 nm.</li></ul><h4>Software</h4><ul><li>The ECOMAC software or <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a> (FDA 21 CFR Part 11 compliant) is internally installed in the PC controlling the system.</li></ul><h4>Customization</h4><ul><li>The unit is completely customizable to the application needs to meet any scale criteria–from small flash sample separation testing through pilot scale prediction to high-capacity production.</li><li>It could work in ranges of 10 ml/min.–6000 ml/min and it can be easily and automatically switched between these scales.</li></ul>

ECOM ECP201LG Gradient Preparative Pump 1000 mL/min

An innovative, powerful, and cost-effective unit for preparative LC separations with high pressure. 4 low pressure gradient valves, flow up to 1000 mL/min. 

<div style="display:block;" aria-labelledby="tab_item-0">The ECP201L Preparative Pump is an innovative, powerful, and cost-effective unit for preparative liquid chromatography where high pressure is needed. The pump with 4 low-pressure valves delivers a flow rate of up to 1000 mL/min at a very high output pressure of up to 15 MPa (2176 psi).<h3>Main features and benefits</h3><h4>Durability</h4>The pump is constructed for high pressure and flow rates. This makes it suitable for day-to-day long-term use in production<h4>Wide application possibilities</h4>The unit is used for pharmaceutical separations and plant extract purification, such as cannabinoids, peptides, and oligonucleotides. Also, it has much wider use in other chemical production, such as dosing into chemical reactors, and in various laboratory uses. Another advantage is the usability of the same device for separation method development.<h4>Smart controls and diagnostics</h4>It is easy to operate, especially with ECOMAC and Clarity software. This makes unit management powerful and convenient. The basic version of the ECOMAC software is available free of charge. It is also used for service and diagnostics.<h4>Other useful features</h4><ul><li>A new learning algorithm for pulsation suppression</li><li>A leakage sensor</li><li>A seal wash kit for longer piston seal life</li><li>Also available in configurations made of certified bio-inert materials</li></ul><h3>Parameters</h3><ul><li>Flow rate: 1 – 1000 ml/min</li><li>Maximum operating pressure in the pump: 15 MPa up to 1000 ml/min</li><li>Flow rate setting: 1 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 1 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Wetted materials in the pump: stainless steel, PEEK, TefzelTM, Kalrez®, ceramic, seals GFP (PTFE) by default or UHMW-PE on request</li><li>Control: RS232, Ethernet(LAN), USB</li><li>Output capillary outer diameter: 1/8 "</li><li>Input tubing outer diameter: 3/8 ”</li><li>Number of gradient valves*: 4 (A,B,C,D)</li><li>Orifice: 5 mm</li><li>Fluid temperature: 5 - 90°C</li><li>Connecting thread: 5/8" - 18 UNF</li><li>Power supply: 100 - 240 V AC</li><li>Power input: 500 VA</li><li>Weight of the complete set: 30 kg (66.14 lb)</li><li>Dimensions (w x h x d) of the complete set: 340 x 170 x 640 mm (13.4 x 6.7 x 25.2 in)</li></ul></div>

ECOM ECP2200 Preparative Pump

Powerful and durable pumps for preparative applications in low- and high-pressure liquid chromatography, designed with 2 pump heads connected in parallel

<div style="display:block;" aria-labelledby="tab_item-0">This pump works as an isocratic pump and together with a gradient box also as a gradient pump.These powerful and durable pumps are suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. They are designed with 2 pump heads connected in parallel with a piston diameter of 3/8”.&nbsp;Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. New material versions have been added-in, beyond to stainless steel A316L, also Hastelloy, titanium and PEEK for biocompatible applications. The pumps can operate in two modes, i.e. using constant pressure or using the newly added option of constant flow rate.&nbsp;<h4>Gradient function:&nbsp;</h4>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes ECB2005, ECB2005PC are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.Customers can use the pistons‘ back-washing function, after working, for example, with a buffered mobile phase.<h4>Seals:&nbsp;</h4>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.<h4>Software support:&nbsp;</h4>The pumps are supported by ECOMAC and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a>.<h3>Features and Benefits</h3><ul><li>All models include a display and keyboard for manual control</li><li>Precise low-pressure gradient mixing (when connected with a gradient module)</li><li>Very good durability and reliability with proper maintenance</li><li>Extended number of wetted material versions – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li>Piston back-washing function for work with buffered or aggressive mobile phases</li><li>New learning algorithm for pulsation suppression</li><li>Integrated leakage sensor</li><li>Heating unit for pump heads available</li><li>Custom pump modifications of request</li></ul>This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).<h4>Accessories:</h4>Pump head heating unit, see the accessories. If you would like this function for your pump, add the heating unit.<h4>Parameters:</h4><ul><li>Flow rate: 0.001 – 250 ml/min</li><li>Maximum operating pressure: 30 MPa (4351 psi) up to 100 ml/min; 15 MPa (2176 psi) at 250 ml/min</li><li>Flow rate setting: 0.001 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 1 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Communication: RS232,Ethernet (LAN),USB,Remote digital I/O</li><li>Power supply: 100 240V 50/60Hz 250VA</li><li>Dimensions W x H x D: 280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li>Weight: 13.5 kg (30 lb)</li><li>Output capillary outer diameter: 1/8”</li><li>Upper pressure limit (MPa): 1– 30 (4351PSI)</li><li>Precision of pressure measurement: ± 2%</li><li>Supported Gradient Box: ECB2005, ECB2005PC</li><li>Pressure Compensation Learning: yes&nbsp;</li></ul></div>

ECP2050 Preparative Pump

Preparative HPLC pump with flow-rate ranges of 0.001 to 50 ml/min. 

<div style="display:block;" aria-labelledby="tab_item-0">This pump works as an isocratic pump and together with a gradient box also as a gradient pump.These powerful and durable pumps are suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. They are designed with 2 pump heads connected in parallel with a piston diameter of 3/8”.Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. The pumps can operate in two modes, i.e. using constant pressure or the newly added option of constant flow rate. New material versions have been added-in beyond to stainless steel A316L, also Hastelloy, titanium and PEEK for biocompatible applications.<h4>Gradient function</h4>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes ECB2007, ECB2007PC are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.Customers can use the pistons‘ back-washing function, after working, for example, with a buffered mobile phase.<h4>Seals</h4>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.<h4>Software support</h4>The pumps are supported by ECOMAC and Clarity software.<h3>Main features and benefits</h3><ul><li>All models include a display and keyboard for manual control</li><li>&nbsp;Precise low-pressure gradient mixing (when connected with a gradient module)</li><li>Very good durability and reliability with proper maintenance</li><li>Extended number of wetted material versions – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li>Piston back-washing function for work with buffered or aggressive mobile phases</li><li>New learning algorithm for pulsation suppression</li><li>Integrated leakage sensor</li><li>Heating unit for pump heads available</li><li>Custom pump modifications of request</li></ul>This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg" alt="ECOM: ECP2050 - Preparative Pump - Pressure Compensation Learning algorithm " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg 188w," sizes="100vw" width="700" height="580"><h3>Optional configurations/variants</h3>Pump head heating unit, see the accessories shown below. In case you would like this function for your pump, add also the heating unit to&nbsp;your cart. (Also can be found in the Accessories product category).<h5>PN / Wetted parts of pumping block</h5><ul><li>ACCH000X / Hastelloy</li><li>ACCP000X /<span style="-webkit-text-stroke-width:0px;display:inline !important;float:none;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;"> PEEK</li><li>ACC0000X / Stainless steel</li><li>ACCT000X / Titanium</li></ul><h4>Parameters:</h4><ul><li>Flow rate: 0.001 – 50 ml/min</li><li>Maximum operating pressure: 30 MPa (4351 psi)</li><li>Flow rate setting: 0.1 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 0,5 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Wetted materials: Stainless steel or Hastelloy or Titanium or PEEK + PEEK, TefzelTM, PE, ceramics, seals&nbsp;</li><li>Communication: RS232, Ethernet (LAN), USB, Remote digital I/O</li><li>Power supply: 100 240V 50/60Hz 250VA</li><li>Dimensions W x H x D: 280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li>Weight: 12 kg (26,5 lb)</li><li>Output capillary outer diameter: 1/16”</li><li>Upper pressure limit (MPa): 0,1 – 30 (4351PSI)</li><li>Precision of pressure measurement: ± 2%</li><li>Supported Gradient Box: ECB2005, ECB2005PC</li><li>Pressure Compensation Learning: yes&nbsp;</li></ul></div>

ECOM ECP2100 Preparative Pump

 Preparative HPLC pump with a flow-rate range up to 100 ml/min. 

<div style="display:block;" aria-labelledby="tab_item-0">Innovated pumps ECP2100 work as isocratic pumps and together with a gradient box also as a gradient pump. These powerful and durable pumps are suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. They are designed with 2 pump heads connected in parallel with a piston diameter of 3/8”.Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. New material versions have been added-in, beyond to stainless steel A316L, also Hastelloy, titanium and PEEK for biocompatible applications.The pumps can operate in two modes, i.e. using constant pressure or using the newly added option of constant flow rate. The ECP2100 pump now has an innovated spring-loaded outlet check-valve.<h4>Gradient function</h4>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes ECB2007, ECB2007PC are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.Customers can use the pistons‘ back-washing function, after working, for example, with a buffered mobile phase.<h4>Seals</h4>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.<h4>Software support</h4>The pumps are supported by ECOMAC and Clarity software.<h3>Main features and benefits</h3><ul><li>All models include a display and keyboard for manual control</li><li>Precise low-pressure gradient mixing (when connected with a gradient module)</li><li>Very good durability and reliability with proper maintenance</li><li>Extended number of wetted material versions – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li>Piston back-washing function for work with buffered or aggressive mobile phases</li><li>New learning algorithm for pulsation suppression</li><li>Integrated leakage sensor</li><li>Heating unit for pump heads available</li><li>Custom pump modifications of request</li></ul>This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg" alt="ECOM: ECP2100 - Preparative Pump - Pressure Compensation Learning algorithm " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg 188w," sizes="100vw" width="700" height="580"><h3>Optional configurations/variants</h3>Pump head heating unit, see the accessories shown below. If you would like this function for your pump, add the heating unit to&nbsp;your cart. (Also can be found in the Accessories product category).PN / Wetted parts of pumping block<ul><li>ACCH000X / Hastelloy</li><li>ACCP000X /<span style="-webkit-text-stroke-width:0px;display:inline !important;float:none;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;"> PEEK</li><li>ACC0000X / Stainless steel</li><li>ACCT000X / Titanium</li></ul><h4>Parameters:</h4><ul><li>Flow rate: 0.1 – 100 ml/min</li><li>Maximum operating pressure: 30 MPa (4351 psi)</li><li>Flow rate setting: 0.001 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 0,5 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Communication: RS232, Ethernet (LAN), USB, Remote digital I/O</li><li>Power supply: 100 240V 50/60Hz 250VA</li><li>Dimensions W x H x D: 280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li>Weight: 12 (26,5 lb)</li><li>Output capillary outer diameter: 1/16”</li><li>Upper pressure limit (MPa): 1– 30 (4351PSI)</li><li>Precision of pressure measurement: ± 2%</li><li>Supported Gradient Box: ECB2005, ECB2005PC</li><li>Display, keypad: yes&nbsp;</li><li>Pressure Compensation Learning: yes</li></ul></div>

ECOM ECP2300 Preparative Pump

<div style="display:block;" aria-labelledby="tab_item-0">Powerful and durable pumps suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. An extended&nbsp;number of wetted material versions – not only stainless steel but also Hastelloy, titanium, and PEEK.&nbsp;ECP2300 are designed with 2 pump heads connected in parallel with a piston diameter of 3/8”.Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. The pumps can operate in two modes, i.e. using constant pressure or using the newly added option of constant flow rate.<h4>Gradient function:&nbsp;</h4>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes ECB2005, and ECB2005PC are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.Customers can use the pistons‘ back-washing function, after working, for example, with a buffered mobile phase.<h4>Seals:&nbsp;</h4>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.<h4>Software support:&nbsp;</h4>The pumps are supported by ECOMAC and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a>.<h3>Features and Benefits</h3><ul><li>All models include a display and keyboard for manual control</li><li>Precise low-pressure gradient mixing (when connected with a gradient module)</li><li>Very good durability and reliability with proper maintenance</li><li>Extended number of wetted material versions – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li>Piston back-washing function for work with buffered or aggressive mobile phases</li><li>New learning algorithm for pulsation suppression</li><li>Integrated leakage sensor</li><li>Custom pump modifications of request</li></ul>This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).Service videos -Seals replacement and&nbsp;Valve replacement :&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.ecomsro.com/support/service-videos">https://www.ecomsro.com/support/service-videos</a><h4>Parameters:</h4><ul><li>Flow rate: 0.001 – 300 ml/min</li><li>Maximum operating pressure: 15 MPa (2176 psi)</li><li>Flow rate setting: 0.001 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 0.5 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Communication: RS232,Ethernet (LAN),USB,Remote digital I/O</li><li>Power supply: 100 240V 50/60Hz 200VA</li><li>Dimensions W x H x D: 280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li>Weight: 13 kg (28.7 lb)</li><li>Output capillary outer diameter: 1/8”</li><li>Upper pressure limit (MPa): 1– 15 (2176PSI)</li><li>Precision of pressure measurement: ± 2%</li><li>Supported Gradient Box: ECB2005, ECB2005PC</li><li>Pressure Compensation Learning: yes&nbsp;</li></ul></div>

ECOM Separtrix PP03 Preparative Pump

Pulse-less triplex piston pumps designed for preparative chromatography applications, equipped with unique compact three-piston head for smooth flow and easy maintenance.

Separtrix PP03 are pulse-less triplex piston pumps designed for preparative chromatography applications - nevertheless, they can be used everywhere, where high pressure, precise flow without pulsations, and high resistance against corrosion are needed. Pumps are equipped with unique compact three-piston head for smooth flow and easy maintenance without a risk of leakage on connecting parts. Back flushed pistons are covered by a hard and chemically resistant polycrystalic carbon layer. Piston cylinders have low dead volume and samples injected through the pump appear low spreading.Pumps with different flow rate ranges differ only in head type, piston diameters, and tubing diameters. Separtrix PP03 are usually supplied with an asynchronous motor and frequency converter but are also available with a step motor which allows the use of the pump in an extremely broad range of flow rates - both for analytical and preparative applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECOM_Separtrix_PP_03_Preparative_Pump_4_6079b99e0f.jpg" alt="ECOM: Separtrix PP03 Preparative Pump" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECOM_Separtrix_PP_03_Preparative_Pump_4_6079b99e0f.jpg 245w," sizes="100vw" width="1349" height="503">Special configurations of Separtrix PP03 pumps are used for three phases low pressure gradient – in this case is extremely important that these pumps offer a nearly constant flow rate not only on the output but on the input too. Low-pressure gradient units are delivered with three solenoid valves on the plate which can be connected to the pump side. The power supply for valves is installed inside the pump box. 10 linear steps can be programmed.Separtrix PP03 pumps are delivered in configuration for high-pressure binary gradient as well. Two pumps – master and slave types – are working together. The master pump controls the flow rate and gradient composition (10 linear steps), slave pump is delivered without a control display, keyboard(s), and pressure sensor. All Separtrix PP03 pumps are equipped with keyboard(s) and graphic display. There is a possibility to set flow rate, pressure limit, and flow correction. A second keyboard is included for gradient programming in the case of low-pressure gradient pumps and high-pressure gradient master pumps.Separtrix PP 03 can be used in the mode of remote control. Pumps can cooperate with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity data station (Dataapex)</a>. Flow rate, gradient composition, and pressure limit can be programmed. The control module for PP 03 pumps was successfully tested and agreed upon by Dataapex. PP 03 pumps can be controlled using Ecom software ECOMAC as well.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECOM_Separtrix_PP_03_Preparative_Pump_2_ce1c6f0f8f.jpg" alt="ECOM: Separtrix PP03 Preparative Pump" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECOM_Separtrix_PP_03_Preparative_Pump_2_ce1c6f0f8f.jpg 245w," sizes="100vw" width="1377" height="832"><h3>Types &amp; Description with technical parameters</h3>SEPARTRIX PP03 A: Piston pulsless pump, three pistons O.D. 10 mm with a diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor vector frequencyconverter, flow rate 25 ml/min. – 800 ml/min., 260 bar (3500 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 B: Piston pulsless pump, three pistons O.D. 14 mm with diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor with vector frequency converter, flow rate 50 ml/min. – 1600 ml/min., 160 bar (2100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 A: Piston pulsless pump, three pistons O.D. 10 mm with a diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor vector frequencyconverter, flow rate 25 ml/min. – 800 ml/min., 260 bar (3500 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 B: Piston pulsless pump, three pistons O.D. 14 mm with diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor with vector frequency converter, flow rate 50 ml/min. – 1600 ml/min., 160 bar (2100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 C: Piston pulsless pump, three pistons O.D. 20 mm with diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor with vector frequency converter, flow rate 100 ml/min. – 3200 ml/min., 80 bar (1100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 AG: PP03 A pump, flow rate 25 ml/min. – 800 ml/min., max. 260 bar (3500 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder + 3 x valve power supply in pump unit), second keyboard for gradient control&nbsp;SEPARTRIX PP03 BG: PP03 B pump, three phases, flow rate 50 ml/min. – 1600 ml/min., max. 150 bar (2100 PSI), low-pressure gradient included (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03 C: Piston pulsless pump, three pistons O.D. 20 mm with diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor with vector frequency converter, flow rate 100 ml/min. – 3200 ml/min., 80 bar (1100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 AG: PP03 A pump, flow rate 25 ml/min. – 800 ml/min., max. 260 bar (3500 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder + 3 x valve power supply in pump unit), second keyboard for gradient control&nbsp;SEPARTRIX PP03 BG: PP03 B pump, three phases, flow rate 50 ml/min. – 1600 ml/min., max. 150 bar (2100 PSI), low-pressure gradient included (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03 CG: PP03 C pump, flow rate 100 ml/min. – 3200 ml/min., 80 bar (1000 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03S A: Piston pulsless pump, three pistons O.D 10 mm with diamond layer in one stainless steel head, ceramic ball valves, step motor, flow rate 1 ml/min. – 400 ml/min., 250 bar (3500 PSI), pressure measurement and limitSEPARTRIX PP03S B: Piston pulsless pump, three pistons O.D 14 mm with diamond layer in one stainless steel head, ceramic ball valves, step motor, flow rate 1 ml/min. – 800 ml/min., 150 bar (2100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03S C: Piston pulsless pump, three pistons O.D 20 mm with diamond layer in one stainless steel head, ceramic ball valves, step motor, flow rate 2 ml/min. – 1600 ml/min., 70 bar (800 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03S AG: PP03S A pump, flow rate 1 ml/min. – 400 ml/min., 250 bar (3500 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03S BG: PP03S B pump, flow rate 1 ml/min. – 800 ml/min., 150 bar (2100 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03S CG: PP03S C pump, flow rate 2 ml/min. – 1600 ml/min., 70 bar (800 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03SM A: PP03S A pump, flow rate 0,5 ml/min. – 400 ml/min., 250 bar (3500 PSI), master pump for binary high-pressure gradient, second keyboard for gradient programming&nbsp;SEPARTRIX PP03SM B: PP03S B pump, flow rate 1 ml/min. – 800 ml/min., 150 bar (2100 PSI), master pump for binary high-pressure gradient, second keyboard for gradient programmingSEPARTRIX PP03SM C: PP03S C pump, flow rate 2 ml/min. – 1600 ml/min., 70 bar (800 PSI), master pump for binary high-pressure gradient, second keyboard for gradient programmingSEPARTRIX PP03SS A: PP03SS A pump, flow rate 0,5 ml/min. – 400 ml/min., 250 bar (3500 PSI), slave pump for binary gradient, no display, no keyboard&nbsp;SEPARTRIX PP03SS B: PP03SS B pump, flow rate 1 ml/min. – 800 ml/min., 150 bar (2100 PSI), slave pump for binary gradient, no display, no keyboardSEPARTRIX PP03SS C: PP03SS C pump, flow rate 2 ml/min. – 1600 ml/min., 70 bar (800 PSI), slave pump for binary gradient, no display, no keyboard

ECOM Separchrom PC01-HPLC Industrial Preparative Columns with Axial Compression

Separchrom PC01 ECOM industrial preparative columns correspond to the highest requirements of preparative liquid chromatography. They are designed to be used in a mode of axial compression with a moving input piston. 

<div style="display:block;" aria-labelledby="tab_item-0">HPLC Columns for prep chromatography. High performance columns with axial compression.Separchrom PC01 ECOM columns correspond to the highest requirements of preparative liquid chromatography. They are designed to be used in a mode of axial compression with a moving input piston. In the original design, they are equipped with identical pistons on input and output (biaxial compression as an option). In the ECOM Separchrom PC FE modification, they have a fixed end plate on the output instead of the piston. Pistons and plates are equipped with frits. A liquid distributing system is installed under each frit. The design guarantees perfect piston flow, high efficiency even for high injection volume, and simple accessibility of frits for cleaning. Columns can be packed by dynamic slurry method or by sedimentation or with sorbent in a dry state.SEPARCHROM PC 01 ECOM columns are made of stainless steel AISI 316L (tube inner surface is polished to reach Ra &lt; 0.3 µm) with stainless steel pistons and UHMWPE (PTFE on the request) sealing rings. The stroke of the main piston is proportional to the used hydraulic cylinder or flange bolt length. Pistons are sectional; the frit (sintered mesh disc 3 µm pore size, 5 mm thickness) with the frit ring can be removed. The output plate in the FE version is made of UHMWPE, frit is fixed with a simple frit ring. Column flanges are not welded, but screwed onto the column tube. No temperature increase during manufacturing means no change in steel properties. The column stand made of stainless steel profiles is included for columns over 100 mm I.D.Hydraulic cylinders fitted on the upper flange are offered with manual oil pumps as well as with fully automated electric motor-driven devices for column packing/unpacking procedures.<h3>Accessories</h3>Columns are offered with a broad scale of accessories such as filling adapters, slurry mixing vessels, slurry transporting pumps, etc.<h3>COLUMN CONFIGURATION EXAMPLES</h3>Columns are custom-made, do not hesitate to ask for any other sizes within the possible ranges<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_1_1795482301.jpg" alt="ECOM: Separchrom PC01-HPLC Column - available configuration - part 1" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_1_1795482301.jpg 236w," sizes="100vw" width="1440" height="952"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_2_6d3198af67.jpg" alt="ECOM: Separchrom PC01-HPLC Column - available configuration - part 2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_2_6d3198af67.jpg 239w," sizes="100vw" width="1442" height="942"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_3_9fd77a1054.jpg" alt="ECOM: Separchrom PC01-HPLC Column - available configuration - part 3" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_3_9fd77a1054.jpg 245w," sizes="100vw" width="1435" height="315"></div>

ECOM Separchrom PC02-MPLC (Medium-pressure) Columns for Prep Chromatography

Industrial-scale Preparative Columns PC02 - Columns for medium-pressure with axial bed compression for prep chromatography.

<div style="display:block;" aria-labelledby="tab_item-0">ECOM Separchrom PC02 medium-pressure stainless steel columns are intended for separation where high pressure is not necessary, but good efficiency can still be expected. They are made of stainless steel tubes.Part of the column is an input piston made of ultra-high molecular weight polyethylene and it is equipped with stainless steel support and stainless steel mesh frit. Flow distributor with groves is incorporated into the piston to guarantee piston flow with nearly zero pressure drop. The design guarantees simple accessibility of frits for cleaning.Columns can be packed by dynamic slurry method, or by sedimentation, or with sorbent in the dry state, both with silica and polymer sorbents. For soft polymer sorbents, ECOM Separchrom PC02 oil systems can be equipped with a special software application to compensate for the swelling and unswelling of sorbents in different mobile phases (the column piston is moved in very small steps up and down during use).<h3>Materials</h3>Medium pressure prep chromatography column ECOM SEPARCHROM PC 02 is made of AISI 316L stainless steel, UHMWE moving pistons are equipped with integral sealing wings. Frits (stainless steel mesh 5 µm pore size, 5 mm thickness) can be fixed with a stainless steel ring. The stand made of stainless steel profiles is included.<h3>COLUMN CONFIGURATION EXAMPLES</h3>Columns are custom-made, do not hesitate to ask for any other sizes within the possible ranges<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_02_HPLC_Column_available_configuration_part_1_dfb61faf03.jpg" alt="ECOM: Separchrom PC02-HPLC Column - available configurations - part 1" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_02_HPLC_Column_available_configuration_part_1_dfb61faf03.jpg 245w," sizes="100vw" width="1641" height="939"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_02_HPLC_Column_available_configuration_part_2_cb9f807757.jpg" alt="ECOM: Separchrom PC02-HPLC Column - available configurations - part 2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_02_HPLC_Column_available_configuration_part_2_cb9f807757.jpg 245w," sizes="100vw" width="1645" height="364"></div>

ECOM Separchrom PC04-Non-metal Columns for Prep Chromatography

Industrial-scale preparative non-metal columns for prep chromatography with axial compression correspond to the highest requirements of preparative liquid chromatography. 

<div style="display:block;" aria-labelledby="tab_item-0">Polyethylene cartridge columns are usually intended for flash chromatography. We offer powerful ECOM Separchrom PC04 columns made of high-quality ultra-high molecular weight polyethylene, equipped with moving pistons and with no metal in contact with the mobile phase. Non-metal columns are required in biochromatography and some other special applications.Plastic columns for preparative liquid chromatography ECOM SEPARCHROM PC 04, are unfilled, and made of ultra-high molecular weight polyethylene. They are equipped with the same pistons on the input and the output. The input piston is moved either by flange bolts (type A), or by a central stainless steel threaded rod (type B). The pistons are fitted with plastic polypropylene frits 10 µm and a distribution system.<h3>COLUMN CONFIGURATION EXAMPLES</h3>Columns are custom-made, do not hesitate to ask for any other sizes within the possible ranges<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_04_HPLC_Column_available_configurations_8767eb40ae.jpg" alt="ECOM: Separchrom PC04-HPLC Column - available configurations" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_04_HPLC_Column_available_configurations_8767eb40ae.jpg 245w," sizes="100vw" width="1645" height="823"></div>

Products focused on PrepLC distributed by ECOM spol. s r.o. - page 2

Related content

4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS

RAFA 2026 - Deadline for Abstract Submission for poster presentation is coming

Industry program at ISC 2026