🎤Dr. Leigh Terry (University of Alabama)In this episode of Concentrating on Chromatography, we sit down with Dr. Leigh Terry from the University of Alabama to explore one of the biggest challenges in environmental analysis today: understanding—and accurately measuring—PFAS in water systems.While most regulatory methods focus on a small number of compounds like PFOA and PFOS, there may be thousands of PFAS compounds in the environment—and much of that burden goes undetected.<h4>In this episode, we cover:</h4><ul><li>Why PFAS are called “forever chemicals” (and why that label is evolving)</li><li>The limitations of targeted LC-MS/MS methods</li><li>What the fluorine mass balance problem reveals about PFAS destruction</li><li>How combustion ion chromatography (CIC) helps measure total organofluorine</li><li>Why “99.99% removal” doesn’t always mean what you think</li><li>Where PFAS actually end up in wastewater treatment systems</li><li>The most effective technologies for PFAS removal today</li><li>Why drinking water may only account for ~20% of total PFAS exposure</li></ul><h4>Key Insight:</h4>Even when analyzing dozens of PFAS compounds, researchers may only be capturing a small fraction of total organic fluorine—raising major questions about how we monitor and regulate these contaminants.<h3>Video Transcription</h3>In this episode of Concentrating on Chromatography, Dr. Leigh Terry discusses PFAS contamination in drinking water, the analytical challenges associated with measuring these compounds, current treatment technologies, and future directions for monitoring and regulation. The conversation highlights how analytical chemistry, environmental engineering, and public health intersect in addressing one of today's most discussed classes of emerging contaminants.<h4>Understanding PFAS and Their Significance</h4><h5>The University of Alabama Water Quality Research Center</h5>Dr. Leigh Terry is part of the University of Alabama's Center for Water Quality Research. The center operates an analytical laboratory focused on drinking water quality and the detection of contaminants at extremely low concentrations.The laboratory specializes in:<ul><li>Drinking water analysis</li><li>Organic contaminants</li><li>Emerging contaminants</li><li>PFAS monitoring</li><li>Water quality research</li></ul>Their work supports both research initiatives and practical solutions for water utilities and environmental agencies.<h4>Why Focus on PFAS?</h4>Although PFAS compounds have existed since the 1940s, public awareness has increased dramatically only in recent years.According to Dr. Terry, several factors contributed to the rapid growth of PFAS research:<ul><li>Increased media attention</li><li>New regulatory initiatives</li><li>Growing evidence of human exposure</li><li>Expanding research opportunities</li><li>Greater understanding of potential health risks</li></ul>PFAS are not new contaminants. Rather, society is only beginning to understand their prevalence in the environment and their potential impacts on human health.<h4>The Development of Standardized PFAS Methods</h4>One major advancement in PFAS analysis has been the development of standardized EPA methods.These methods allow:<ul><li>Consistent testing procedures</li><li>Comparable results between laboratories</li><li>Regulatory monitoring</li><li>Reliable data interpretation</li></ul>Dr. Terry notes that many of these standardized approaches began appearing around 2018–2019, providing a common framework for analytical laboratories and researchers.<h4>How Large Is the PFAS Universe?</h4>Many people are familiar with PFOA and PFOS, two of the most heavily studied PFAS compounds. However, these represent only a small fraction of the broader PFAS family.PFOA and PFOS are often referred to as:<ul><li>Legacy PFAS compounds</li><li>Original PFAS contaminants</li><li>Early commercial PFAS chemicals</li></ul>The challenge is that even minor modifications to molecular structure create entirely new PFAS compounds.Researchers estimate:<ul><li>Thousands of PFAS compounds exist</li><li>Some estimates exceed 10,000 compounds</li><li>Exact numbers depend on how PFAS are defined</li></ul>Scientific debate continues regarding classification and definition, making precise counting difficult.<h4>Why PFAS Are Called "Forever Chemicals"</h4>Dr. Terry notes that the phrase "forever chemicals" has become widely used, although she suggests the terminology could benefit from rebranding.PFAS persistence results from two key chemical properties.<h5>1. Amphiphilic Structure</h5>PFAS molecules contain:<ul><li>A water-loving (hydrophilic) head group</li><li>A water-repelling (hydrophobic) fluorinated tail</li></ul>This unusual combination allows PFAS to partition between:<ul><li>Water</li><li>Solids</li><li>Air-water interfaces</li><li>Foam</li></ul>Unlike many organic compounds, PFAS can interact effectively with multiple environmental phases.<h5>2. Extremely Strong Carbon–Fluorine Bonds</h5>The carbon–fluorine bond is among the strongest bonds in organic chemistry.As Dr. Terry explains:<ul><li>Large amounts of energy are required to break these bonds</li><li>Natural degradation processes are often ineffective</li><li>Destruction can require highly aggressive treatment conditions</li></ul>These properties make PFAS extremely persistent in both environmental systems and biological organisms.<h4>Toxicity, Regulations, and Environmental Behavior</h4><h5>Understanding PFAS Toxicity</h5>One of the biggest challenges in PFAS regulation is the incomplete understanding of toxicity.Dr. Terry explains that EPA regulations generally consider three factors:<ul><li>Presence in drinking water sources</li><li>Toxicity to humans</li><li>Availability of practical treatment options</li></ul>While some PFAS compounds are known to be harmful, many others remain insufficiently studied. Furthermore, PFAS often occur as mixtures rather than individual compounds.This creates important scientific questions:<ul><li>What is the toxicity of individual PFAS?</li><li>What is the toxicity of PFAS mixtures?</li><li>How should cumulative exposure be evaluated?</li></ul>These uncertainties continue to drive active research.<h4>What Happens to PFAS in Wastewater Treatment Plants?</h4>PFAS do not behave like many conventional contaminants.Rather than remaining fully dissolved in water, they often accumulate at interfaces such as:<ul><li>Water-solid interfaces</li><li>Water-air interfaces</li><li>Foam layers</li><li>Biological solids</li></ul>As a result:<ul><li>Some PFAS accumulate in biosolids</li><li>Some partition into foam</li><li>Some remain in treated effluent</li></ul>Their distribution depends on wastewater composition and treatment processes.<h4>Regulatory Monitoring and Targeted LC-MS Analysis</h4>Current EPA regulations focus on targeted analysis of specific PFAS compounds.Examples include:<ul><li>PFOA</li><li>PFOS</li></ul>Drinking water regulations currently establish maximum contaminant levels (MCLs) for these compounds at extremely low concentrations.Advantages of targeted LC-MS analysis include:<ul><li>Clearly defined analytes</li><li>Established regulatory limits</li><li>High analytical sensitivity</li><li>Reliable quantitation</li></ul>However, limitations remain:<ul><li>Only a small subset of PFAS is measured</li><li>Many PFAS compounds remain undetected</li><li>Total PFAS burden may be underestimated</li></ul>As Dr. Terry notes, targeted analysis provides only part of the overall picture.<h4>The Fluorine Mass Balance Problem</h4>One of the most important concepts discussed in the interview is fluorine mass balance.When PFAS degrade, fluorinated fragments may remain in the environment.This creates a critical question:Are treatment technologies truly destroying PFAS, or merely transforming them into shorter-chain PFAS compounds?To answer this, researchers perform fluorine mass balance calculations using:<ul><li>Targeted PFAS analysis</li><li>Fluoride measurements</li><li>Total organic fluorine measurements</li></ul>A successful destruction process should ultimately convert PFAS into fluoride ions rather than simply producing smaller PFAS molecules.<h4>Advanced Analysis, Treatment Technologies, and Future Directions</h4><h5>Combining Targeted and Bulk Fluorine Measurements</h5>Dr. Terry's group complements targeted LC-MS analysis with measurements of total organic fluorine.Approaches include:<ul><li>Absorbable Organic Fluorine (AOF)</li><li>Extractable Organic Fluorine (EOF)</li><li>Combustion Ion Chromatography (CIC)</li></ul>In these methods:<ul><li>Organic fluorine is captured</li><li>Samples are combusted</li><li>Released fluoride is quantified</li></ul>This provides insight into total fluorinated content, although contributions may originate from sources beyond PFAS, including pharmaceuticals and consumer products.<h4>Surprising Findings from Combined Analysis</h4>One important finding from wastewater studies was that targeted PFAS measurements accounted for only a small fraction of total organic fluorine.Dr. Terry reports that:<ul><li>Approximately 4% of measured AOF could be explained by targeted PFAS compounds</li><li>The majority of organic fluorine remained unidentified</li></ul>This suggests that environmental fluorine chemistry is considerably more complex than currently represented by routine monitoring programs.<h4>Preventing PFAS Contamination in the Laboratory</h4>PFAS analysis requires exceptionally strict quality control.Dr. Terry's laboratory routinely:<ul><li>Tests every new batch of SPE cartridges</li><li>Tests carbon tubes before use</li><li>Uses DI water blanks</li><li>Verifies consumables labeled as PFAS-free</li><li>Collects field blanks</li><li>Monitors analytical blanks throughout workflows</li></ul>These practices help prevent contamination from laboratory materials and consumables, a common challenge in ultra-trace PFAS analysis.<h4>Current PFAS Treatment Technologies</h4>Three technologies currently dominate PFAS removal in drinking water treatment:<ul><li>Granular Activated Carbon (GAC)</li><li>Ion Exchange Resins</li><li>Reverse Osmosis (RO)</li></ul>Selection depends on:<ul><li>PFAS concentration</li><li>Water quality</li><li>Local operating conditions</li><li>Utility infrastructure</li></ul>Although many emerging technologies are being investigated, these remain the most widely implemented solutions.<h4>Membrane Filtration and Pretreatment</h4>Dr. Terry collaborates with colleagues studying membrane-based PFAS treatment.Her own work focuses on pretreatment through biological filtration.The strategy involves:<ul><li>Removing natural organic matter</li><li>Reducing membrane fouling</li><li>Improving membrane performance</li><li>Extending operational efficiency</li></ul>This integrated approach demonstrates how multiple treatment technologies can work together to improve PFAS removal.<h4>Regional Monitoring Projects</h4>The University of Alabama team participates in EPA-supported monitoring efforts along the Gulf Coast.Their observations indicate:<ul><li>PFAS rarely occur alone</li><li>Multiple PFAS compounds frequently co-occur</li><li>Contamination often originates from identifiable point sources</li></ul>Examples include:<ul><li>Industrial facilities</li><li>Manufacturing operations</li><li>Military installations</li><li>Carpet manufacturing facilities</li></ul>Source identification remains a critical component of environmental monitoring programs.<h4>Future Directions for PFAS Monitoring</h4>Looking ahead, Dr. Terry sees several opportunities for improvement.Desired advances include:<ul><li>In situ monitoring technologies</li><li>Portable detection systems</li><li>Reduced analytical complexity</li><li>Lower instrument costs</li><li>Less expensive consumables</li><li>Faster analysis workflows</li></ul>Current PFAS testing requires substantial expertise, specialized instrumentation, and rigorous quality control, making widespread implementation challenging.<h4>Dr. Terry's Perspective on Long-Term Solutions</h4>When asked what major PFAS challenge she would most like to solve, Dr. Terry offered a broader perspective.Rather than focusing solely on remediation, she emphasized prevention.Her view is that society should:<ul><li>Better evaluate chemical toxicity before commercialization</li><li>Assess risks before large-scale deployment</li><li>Reduce introduction of harmful compounds into the environment</li><li>Support utilities tasked with contaminant removal</li></ul>She notes that water utilities often bear the responsibility of removing contaminants they did not create, highlighting the need for broader societal and regulatory support.<h4>Final Thoughts</h4>Dr. Terry encourages consumers to:<ul><li>Learn where their drinking water originates</li><li>Read annual water quality reports</li><li>Stay informed about local water quality</li><li>Consider appropriate home filtration where needed</li></ul>At the same time, she emphasizes confidence in municipal water systems and the importance of continued investment in water quality research, monitoring, and treatment technologies.<h4>Conclusion</h4>PFAS remain one of the most complex challenges in environmental chemistry and water treatment. Through advanced analytical methods such as LC-MS/MS, combustion ion chromatography, fluorine mass balance approaches, and large-scale monitoring programs, researchers like Dr. Leigh Terry are helping improve our understanding of these persistent contaminants.The interview highlights that solving the PFAS challenge will require a combination of better analytical tools, improved treatment technologies, stronger regulatory frameworks, and a greater emphasis on prevention before contaminants enter the environment.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

PFAS in Water: Why We’re Missing 96% of the Problem

This study evaluates an immobilized enzyme reactor (IMER) containing peptide N-glycosidase from Rudaea cellulosilytica (PNGase Rc) for rapid online deglycosylation of intact glycoproteins. Human glycoproteins and therapeutic antibodies were used as model substrates to assess reactor performance.The PNGase Rc IMER efficiently removed complex sialylated and core-fucosylated glycans, simplifying protein characterization and glycosylation-site analysis. The approach offers a fast and effective solution for bioanalytical workflows, biopharmaceutical quality control, and stability testing of therapeutic proteins.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577">Online Deglycosylation of Monomeric Intact Proteins Using the PNGase Rc Immobilized-Enzyme Reactor</a></h4>Katarína Molnárová, Petr Novák, Jana Nováková, and Petr Pompach*ACS Omega 2026, 11, 17, 25724–25729<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acsomega.6c00577">https://doi.org/10.1021/acsomega.6c00577</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.<div>Protein glycosylation, in which monosaccharide units are attached to a protein backbone through enzymatic pathways, is involved in many important biochemical processes and is considered one of the most important post-translational modifications. <a href="javascript:void(0);">(1)</a> It affects not only the stability and secretion of proteins, cell–cell interactions, and receptor–ligand recognition mechanisms but also the biological activity, plasma half-life, immunogenicity, pharmacodynamics, and pharmacokinetics of biotherapeutics. <a href="javascript:void(0);">(2−5)</a> Therapeutical monoclonal antibodies (mAbs) are among the best known pharmaceuticals that are based on glycoproteins. <a href="javascript:void(0);">(6)</a> Over 90% of the mAbs are derived from immunoglobulin G (IgG), in which N-glycosylation occurs at Asn297 of the Fc region and is responsible for modulating immune responses and interactions with other proteins. <a href="javascript:void(0);">(7,8)</a> In cancer treatment, mAbs are used to increase the response of the immune system against the tumor cell, thus inhibiting the cancerous cell growth and helping with the destruction and elimination. <a href="javascript:void(0);">(9)</a> Among the most commonly used mAbs are trastuzumab, bevacizumab, cetuximab, or margetuximab, which are used as an additional treatment for breast, colorectal, prostate, or lung cancer. <a href="javascript:void(0);">(10)</a>Mass spectrometry (MS)-based methods have become a method of choice in the analysis of mAbs that can provide information on sequence, post-translational modifications, and glycosylation profiles. <a href="javascript:void(0);">(11)</a> The enzymatic removal of the attached glycans from the protein backbone can reduce the complexity of the MS analysis, which can lead to a better understanding of the protein structure and modifications. In glycoproteomics, peptide-N-glycosidase F (PNGase F) is most used for glycan removal. It cleaves the linkage between the Asn residue and the N-acetylglucosamine (GlcNAc). <a href="javascript:void(0);">(12)</a> Jmeian et al. developed a monolithic PNGase F enzyme reactor fabricated in fused silica using a glycidyl methacrylate-co-ethylene dimethacrylate polymer and integrated it into an LC–MS workflow. The authors demonstrated the applicability of this system for the characterization of released neutral and acidic N-glycans by LC–MS analysis. <a href="javascript:void(0);">(13)</a> In another study, oriented immobilization of PNGase F onto a methacrylate-based monolithic support via glutathione-S-transferase–glutathione affinity coupling was employed to release N-glycans from denatured ribonuclease B and fetuin, as well as from native IgG. The released glycans and the corresponding deglycosylated proteins were subsequently analyzed by MALDI-MS. <a href="javascript:void(0);">(14)</a>Gramlich et al. showed the potential of PNGase Rc for N-glycan hydrolysis from both tryptic peptides and native glycoproteins at a low pH. The favorable properties of PNGase Rc, including activity at acidic pH and compatibility with reducing agents such as tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and denaturants like urea, make this enzyme well suited for online intact protein deglycosylation workflows. <a href="javascript:void(0);">(15,16)</a>In this report, we demonstrate the potential of the PNGase Rc IMER for rapid online deglycosylation of reduced monomeric glycoproteins, including human haptoglobin (Hp), human transferrin, anti-hHD6, and the therapeutic monoclonal antibodies trastuzumab and bevacizumab. The column retained its activity over more than 150 consecutive measurements, highlighting its robustness, suitability for repeated use in bioanalytical workflows, and applicability to pharmaceutical research and development.<h4>Experimental Section</h4><h5>Instrumentation and Experimental Conditions</h5>Automated sample handling and online deglycosylation were carried out using the HDX workstation (AffiPro, Czech Republic). The scheme of the system is shown on <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig1">Figure 1</a>. Samples of 50 mM ammonium bicarbonate buffer (pH 8.2) and 40 mM TCEP were placed in a sample tray at 4 °C. Ten microliters of the protein sample at a concentration of 0.03 mg/mL (0.3 μg per injection) were added to 40 μL of 50 mM ammonium bicarbonate and then transferred to 50 μL of 40 mM TCEP, pH 2.3. The disulfide bond reduction was carried at 40 °C for 5 min. The automated sample handling system injected 100 μL of the reaction mixture into the sample loop and switched the injection valve. Online deglycosylation was performed at room temperature using a PNGase Rc IMER (2.1 × 20 mm, AffiPro, Czech Republic) at a flow rate of 50 μL/min, followed by protein desalting on an OPTI-TRAP (0.5 × 2 mm) trap column (Optimize Technologies Inc., Oregon City, Oregon, USA) heated to 55 °C for 10 min with 0.2% formic acid delivered by an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1260+Infinity+III+isocratic+pump">Agilent 1260 Infinity III isocratic pump</a> (Agilent Technologies, Santa Clara, California, USA). By switching the trap valve, the protein was eluted by an acetonitrile gradient [(min)/% B] 0/10-9/10-16/70-16.5/90-17/90-18/10-23/10] delivered by the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+II+LC">Agilent 1290 Infinity II HPLC</a> system (Agilent Technologies, Santa Clara, California, USA) at a flow rate of 20 μL/min. The mobile phase A consisted of 5% acetonitrile with 1% propan-2-ol and 0.1% formic acid, and the mobile phase B consisted of 95% acetonitrile with 1% propan-2-ol and 0.1% formic acid. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=8&amp;search=maXis+Q-TOF">maXis Q-TOF mass spectrometer</a> (Bruker Daltonics, Bremen, Germany) was operated in the survey MS mode in a mass range of m/z 50–3000. The acquired data were analyzed and processed by DataAnalysis 4.4 (Bruker Daltonics) and deconvoluted by UniDec <a href="javascript:void(0);">(17)</a> with the following parameters: data processing range, m/z 500–3000; charge range, 1–80; allowed mass range, 1,000–80,000; sampling rate, 10 Da; peak detection range, 500 Da; and peak detection threshold, 0.1.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_17_25724_25729_Figure_1_Schematic_of_the_automated_sample_handling_system_for_online_protein_deglycosylation_and_desalting_d4ee48b627.jpg" alt="ACS Omega 2026, 11, 17, 25724–25729: Figure 1. Schematic of the automated sample handling system for online protein deglycosylation and desalting. Protein samples, along with 50 mM ammonium bicarbonate and 40 mM TCEP stock solutions were placed in a sample tray chilled to 4 °C, while a second tray heated to 40 °C was used for disulfide bond reduction with TCEP buffer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_17_25724_25729_Figure_1_Schematic_of_the_automated_sample_handling_system_for_online_protein_deglycosylation_and_desalting_d4ee48b627.jpg 245w," sizes="100vw" width="1287" height="386"><h4>Results and Discussion</h4>The performance of the PNGase Rc IMER for the removal of complex N-glycans from intact proteins was tested using heavily glycosylated human Hp with four glycosylation sites (Asn184, Asn207, Asn211, and Asn241) <a href="javascript:void(0);">(18)</a> located at the β-chain and occupied by complex N-glycans terminated with sialic acid. Haptoglobin phenotype 2-1, used in this study, forms multimers consisting of β-chain and disulfide linked α1 and α2 chains. <a href="javascript:void(0);">(19)</a> The detection of glycosylated Hp β-chain requires reduction of the disulfide bonds by incubating the protein with a reducing reagent. TCEP is a common reducing reagent used for fast reduction of disulfide bonds at various pH levels. Forty degrees Celsius, the maximum temperature of the PAL tray, and 5 min of incubation were sufficient for complete reduction of Hp disulfide bonds. Individual signals corresponding to the glycosylated β-chain were observed in the m/z range of 35,800–37,400 (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig2">Figure 2</a>a). The limited resolution of the mass spectrometer did not allow assigning the different glycoforms of the haptoglobin β-chain, which is occupied by biantennary, triantennary, or tetraantennary glycans with various amounts of sialic acid and fucose. <a href="javascript:void(0);">(20,21)</a> The nonglycosylated α1 and α2 chains were observed at m/z 9190 and 15,940. When the PNGase Rc IMER was integrated into the flow system, a signal at m/z 27,270 was detected, demonstrating complete deglycosylation of the haptoglobin β-chain (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig2">Figure 2</a>b).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_17_25724_25729_Figure_2_A_Mass_spectrum_of_human_Hp_without_the_PN_Gase_Rc_IMER_implemented_in_the_system_0ecca84ed4.jpg" alt="ACS Omega 2026, 11, 17, 25724–25729: Figure 2. (A) Mass spectrum of human Hp without the PNGase Rc IMER implemented in the system. The base peak signals of the spectrum correspond to both α1 and α2 chains. The signal of the β chain is spread across its different glycoforms. (B) Mass spectrum of human Hp with the PNGase Rc IMER implemented in the system. The signal of the deglycosylated β chain is higher compared to its glycosylated form and observed only at m/z 27,270. This indicates complete removal of glycans from the Hp β chain." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_17_25724_25729_Figure_2_A_Mass_spectrum_of_human_Hp_without_the_PN_Gase_Rc_IMER_implemented_in_the_system_0ecca84ed4.jpg 245w," sizes="100vw" width="2015" height="913">In a previous study by Krenkova et al., the PNGase F IMER was used for deglycosylation of IgG, RNase B, and bovine fetuin with follow-up mass spectrometry detection of both released glycans and intact proteins using MALDI-TOF MS. <a href="javascript:void(0);">(14)</a> The authors demonstrated an effective method for rapid protein deglycosylation, which, however, requires additional sample handling prior to mass spectrometry measurement. The other study by Jmeian et al., which focused on released N-glycans, implemented a PNGase F IMER with online LC–MS detection. <a href="javascript:void(0);">(13)</a> The authors show the effectiveness of the method by identifying many different glycans released from a single protein or from a complex plasma mixture. Our approach is not directly intended for released N-glycan analysis; instead, it demonstrates the ability to measure intact proteins after rapid online deglycosylation using PNGase Rc at low pH. Samples prepared immediately prior to mass spectrometry analysis using the automated sample-handling platform can reduce variability associated with autosampler residence time, as some proteins may undergo degradation or precipitation during storage in the autosampler.Although all glycoproteins in this study were effectively deglycosylated, the method’s efficiency depends on the characteristics of the individual glycoprotein. Therefore, optimizing method parameters─including the isocratic flow rate, glycoprotein incubation time with TCEP, and/or the addition of denaturing reagent─may improve the overall efficiency of the deglycosylation process.The PNGase Rc IMER stability over a series of injections was tested by using the trastuzumab antibody. The measurements were carried out with the enzyme column kept at the laboratory temperature. As shown in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig5">Figure 5</a>, the results indicate that the column performance remained stable over the course of the 150 measurements, and no glycosylated form of the trastuzumab antibody heavy chain was detected. The activity of PNGase Rc in the presence of 5% acetonitrile and 5% propan-2-ol was evaluated using a bottom-up approach with human 2–1 haptoglobin, which was online-digested by a coimmobilized nepenthesin-2/pepsin IMER. In the absence of an organic solvent, the peptide IEKVVLHPNYSQVD remained fully deglycosylated throughout 30 injections. In contrast, in the presence of organic solvent, a marked loss of enzymatic activity was observed after only a few injections (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.6c00577/suppl_file/ao6c00577_si_001.pdf">Figure 2</a>).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_17_25724_25729_Figure_5_Monitoring_the_stability_and_performance_of_PN_Gase_Rc_IMER_4a9b5a9c53.jpg" alt="ACS Omega 2026, 11, 17, 25724–25729: Figure 5. Monitoring the stability and performance of PNGase Rc IMER. Trastuzumab heavy chain was completely deglycosylated after 150 injections." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_17_25724_25729_Figure_5_Monitoring_the_stability_and_performance_of_PN_Gase_Rc_IMER_4a9b5a9c53.jpg 170w," sizes="100vw" width="996" height="918"><h4>Conclusions</h4>Here, we demonstrate the potential of the PNGase Rc IMER for the online removal of N-glycans from intact monomeric glycoproteins reduced with TCEP. The column showed high sensitivity toward Hp, which has multiple glycosylation sites with various glycan structures, confirming its suitability for the profiling of complex glycoproteins. Bevacizumab and trastuzumab exhibited similar glycosylation profiles, with core-fucosylated biantennary glycans being the most abundant. Online deglycosylation with the PNGase Rc column resulted in a complete removal of the glycans from the heavy chains of both antibodies, proving that the column can be used for the rapid online deglycosylation of intact therapeutic mAbs. Moreover, the column remained stable over 150 measurements without any loss of activity, even when it was maintained at laboratory temperature. The significantly reduced reaction time, together with full automation and the absence of manual handling steps, substantially improves analytical throughput compared to conventional off-line deglycosylation workflows. These findings highlight the potential of the PNGase Rc IMER in the development of glycoprotein-based biopharmaceuticals, where detailed structural characterization of the protein part is critical for quality control, monitoring batch-to-batch variability, and stability testing.</div>

Online Deglycosylation of Monomeric Intact Proteins Using the PNGase Rc Immobilized-Enzyme Reactor

In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars">👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</a></h4>👉 Search and filter in the largest global database with over 5 500 GC, LC, MS and spectroscopy webinars.</blockquote><h5>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.<h5>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.<h5>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.<h5>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.<h5>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.<h5>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.

Webinars LabRulezLCMS Week 27/2026

Article

Webinars

Video

Scientists

Environment

Water

Scientific article

Interview

Science and research

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

ECOM PREPSYSTEM - Chromatography Separation System

A complete flash/preparative/pilot scale/production modular system ready for customizable to the application needs.

We would like to introduce an ECOM product news – a complete flash/preparative/pilot scale/production modular system with an excellent price/performance ratio.<h3>Applications</h3>The system can be used e.g. in:<ul><li>high-capacity systems or with centrifugal liquid/liquid chromatography systems, like in pharmaceutical purification of plant extracts – e.g. cannabinoids (CBD, CBN, CBG, CBC, THC, etc.), peptides, and oligonucleotides, algae extracts, oleoresins, etc.,&nbsp;</li><li>Also in new molecule development or synthesis.</li></ul><h3>Main parts</h3><h4>Pumps</h4><ul><li>The device has 3 pumps, 2 main ones are intended for a "high" pressure gradient (parallel pump connection), and one of them has a 6-solvent selection valve.&nbsp;</li><li>An optional third pump is used for repetitive sampling of high-volume samples. The unit also has a loop sample injection valve and a switching valve for CCC/CPC applications (ascending/descending mode).&nbsp;</li><li>The unit's universal PDA detector measures absorbance at four wavelengths at the same time up to 800 nm.</li></ul><h4>Software</h4><ul><li>The ECOMAC software or <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a> (FDA 21 CFR Part 11 compliant) is internally installed in the PC controlling the system.</li></ul><h4>Customization</h4><ul><li>The unit is completely customizable to the application needs to meet any scale criteria–from small flash sample separation testing through pilot scale prediction to high-capacity production.</li><li>It could work in ranges of 10 ml/min.–6000 ml/min and it can be easily and automatically switched between these scales.</li></ul>

ECOM ECP201LG Gradient Preparative Pump 1000 mL/min

An innovative, powerful, and cost-effective unit for preparative LC separations with high pressure. 4 low pressure gradient valves, flow up to 1000 mL/min. 

<div style="display:block;" aria-labelledby="tab_item-0">The ECP201L Preparative Pump is an innovative, powerful, and cost-effective unit for preparative liquid chromatography where high pressure is needed. The pump with 4 low-pressure valves delivers a flow rate of up to 1000 mL/min at a very high output pressure of up to 15 MPa (2176 psi).<h3>Main features and benefits</h3><h4>Durability</h4>The pump is constructed for high pressure and flow rates. This makes it suitable for day-to-day long-term use in production<h4>Wide application possibilities</h4>The unit is used for pharmaceutical separations and plant extract purification, such as cannabinoids, peptides, and oligonucleotides. Also, it has much wider use in other chemical production, such as dosing into chemical reactors, and in various laboratory uses. Another advantage is the usability of the same device for separation method development.<h4>Smart controls and diagnostics</h4>It is easy to operate, especially with ECOMAC and Clarity software. This makes unit management powerful and convenient. The basic version of the ECOMAC software is available free of charge. It is also used for service and diagnostics.<h4>Other useful features</h4><ul><li>A new learning algorithm for pulsation suppression</li><li>A leakage sensor</li><li>A seal wash kit for longer piston seal life</li><li>Also available in configurations made of certified bio-inert materials</li></ul><h3>Parameters</h3><ul><li>Flow rate: 1 – 1000 ml/min</li><li>Maximum operating pressure in the pump: 15 MPa up to 1000 ml/min</li><li>Flow rate setting: 1 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 1 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Wetted materials in the pump: stainless steel, PEEK, TefzelTM, Kalrez®, ceramic, seals GFP (PTFE) by default or UHMW-PE on request</li><li>Control: RS232, Ethernet(LAN), USB</li><li>Output capillary outer diameter: 1/8 "</li><li>Input tubing outer diameter: 3/8 ”</li><li>Number of gradient valves*: 4 (A,B,C,D)</li><li>Orifice: 5 mm</li><li>Fluid temperature: 5 - 90°C</li><li>Connecting thread: 5/8" - 18 UNF</li><li>Power supply: 100 - 240 V AC</li><li>Power input: 500 VA</li><li>Weight of the complete set: 30 kg (66.14 lb)</li><li>Dimensions (w x h x d) of the complete set: 340 x 170 x 640 mm (13.4 x 6.7 x 25.2 in)</li></ul></div>

ECOM ECP2200 Preparative Pump

Powerful and durable pumps for preparative applications in low- and high-pressure liquid chromatography, designed with 2 pump heads connected in parallel

<div style="display:block;" aria-labelledby="tab_item-0">This pump works as an isocratic pump and together with a gradient box also as a gradient pump.These powerful and durable pumps are suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. They are designed with 2 pump heads connected in parallel with a piston diameter of 3/8”.&nbsp;Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. New material versions have been added-in, beyond to stainless steel A316L, also Hastelloy, titanium and PEEK for biocompatible applications. The pumps can operate in two modes, i.e. using constant pressure or using the newly added option of constant flow rate.&nbsp;<h4>Gradient function:&nbsp;</h4>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes ECB2005, ECB2005PC are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.Customers can use the pistons‘ back-washing function, after working, for example, with a buffered mobile phase.<h4>Seals:&nbsp;</h4>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.<h4>Software support:&nbsp;</h4>The pumps are supported by ECOMAC and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a>.<h3>Features and Benefits</h3><ul><li>All models include a display and keyboard for manual control</li><li>Precise low-pressure gradient mixing (when connected with a gradient module)</li><li>Very good durability and reliability with proper maintenance</li><li>Extended number of wetted material versions – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li>Piston back-washing function for work with buffered or aggressive mobile phases</li><li>New learning algorithm for pulsation suppression</li><li>Integrated leakage sensor</li><li>Heating unit for pump heads available</li><li>Custom pump modifications of request</li></ul>This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).<h4>Accessories:</h4>Pump head heating unit, see the accessories. If you would like this function for your pump, add the heating unit.<h4>Parameters:</h4><ul><li>Flow rate: 0.001 – 250 ml/min</li><li>Maximum operating pressure: 30 MPa (4351 psi) up to 100 ml/min; 15 MPa (2176 psi) at 250 ml/min</li><li>Flow rate setting: 0.001 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 1 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Communication: RS232,Ethernet (LAN),USB,Remote digital I/O</li><li>Power supply: 100 240V 50/60Hz 250VA</li><li>Dimensions W x H x D: 280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li>Weight: 13.5 kg (30 lb)</li><li>Output capillary outer diameter: 1/8”</li><li>Upper pressure limit (MPa): 1– 30 (4351PSI)</li><li>Precision of pressure measurement: ± 2%</li><li>Supported Gradient Box: ECB2005, ECB2005PC</li><li>Pressure Compensation Learning: yes&nbsp;</li></ul></div>

ECP2050 Preparative Pump

Preparative HPLC pump with flow-rate ranges of 0.001 to 50 ml/min. 

<div style="display:block;" aria-labelledby="tab_item-0">This pump works as an isocratic pump and together with a gradient box also as a gradient pump.These powerful and durable pumps are suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. They are designed with 2 pump heads connected in parallel with a piston diameter of 3/8”.Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. The pumps can operate in two modes, i.e. using constant pressure or the newly added option of constant flow rate. New material versions have been added-in beyond to stainless steel A316L, also Hastelloy, titanium and PEEK for biocompatible applications.<h4>Gradient function</h4>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes ECB2007, ECB2007PC are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.Customers can use the pistons‘ back-washing function, after working, for example, with a buffered mobile phase.<h4>Seals</h4>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.<h4>Software support</h4>The pumps are supported by ECOMAC and Clarity software.<h3>Main features and benefits</h3><ul><li>All models include a display and keyboard for manual control</li><li>&nbsp;Precise low-pressure gradient mixing (when connected with a gradient module)</li><li>Very good durability and reliability with proper maintenance</li><li>Extended number of wetted material versions – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li>Piston back-washing function for work with buffered or aggressive mobile phases</li><li>New learning algorithm for pulsation suppression</li><li>Integrated leakage sensor</li><li>Heating unit for pump heads available</li><li>Custom pump modifications of request</li></ul>This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg" alt="ECOM: ECP2050 - Preparative Pump - Pressure Compensation Learning algorithm " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg 188w," sizes="100vw" width="700" height="580"><h3>Optional configurations/variants</h3>Pump head heating unit, see the accessories shown below. In case you would like this function for your pump, add also the heating unit to&nbsp;your cart. (Also can be found in the Accessories product category).<h5>PN / Wetted parts of pumping block</h5><ul><li>ACCH000X / Hastelloy</li><li>ACCP000X /<span style="-webkit-text-stroke-width:0px;display:inline !important;float:none;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;"> PEEK</li><li>ACC0000X / Stainless steel</li><li>ACCT000X / Titanium</li></ul><h4>Parameters:</h4><ul><li>Flow rate: 0.001 – 50 ml/min</li><li>Maximum operating pressure: 30 MPa (4351 psi)</li><li>Flow rate setting: 0.1 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 0,5 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Wetted materials: Stainless steel or Hastelloy or Titanium or PEEK + PEEK, TefzelTM, PE, ceramics, seals&nbsp;</li><li>Communication: RS232, Ethernet (LAN), USB, Remote digital I/O</li><li>Power supply: 100 240V 50/60Hz 250VA</li><li>Dimensions W x H x D: 280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li>Weight: 12 kg (26,5 lb)</li><li>Output capillary outer diameter: 1/16”</li><li>Upper pressure limit (MPa): 0,1 – 30 (4351PSI)</li><li>Precision of pressure measurement: ± 2%</li><li>Supported Gradient Box: ECB2005, ECB2005PC</li><li>Pressure Compensation Learning: yes&nbsp;</li></ul></div>

ECOM ECP2100 Preparative Pump

 Preparative HPLC pump with a flow-rate range up to 100 ml/min. 

<div style="display:block;" aria-labelledby="tab_item-0">Innovated pumps ECP2100 work as isocratic pumps and together with a gradient box also as a gradient pump. These powerful and durable pumps are suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. They are designed with 2 pump heads connected in parallel with a piston diameter of 3/8”.Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. New material versions have been added-in, beyond to stainless steel A316L, also Hastelloy, titanium and PEEK for biocompatible applications.The pumps can operate in two modes, i.e. using constant pressure or using the newly added option of constant flow rate. The ECP2100 pump now has an innovated spring-loaded outlet check-valve.<h4>Gradient function</h4>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes ECB2007, ECB2007PC are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.Customers can use the pistons‘ back-washing function, after working, for example, with a buffered mobile phase.<h4>Seals</h4>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.<h4>Software support</h4>The pumps are supported by ECOMAC and Clarity software.<h3>Main features and benefits</h3><ul><li>All models include a display and keyboard for manual control</li><li>Precise low-pressure gradient mixing (when connected with a gradient module)</li><li>Very good durability and reliability with proper maintenance</li><li>Extended number of wetted material versions – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li>Piston back-washing function for work with buffered or aggressive mobile phases</li><li>New learning algorithm for pulsation suppression</li><li>Integrated leakage sensor</li><li>Heating unit for pump heads available</li><li>Custom pump modifications of request</li></ul>This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg" alt="ECOM: ECP2100 - Preparative Pump - Pressure Compensation Learning algorithm " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg 188w," sizes="100vw" width="700" height="580"><h3>Optional configurations/variants</h3>Pump head heating unit, see the accessories shown below. If you would like this function for your pump, add the heating unit to&nbsp;your cart. (Also can be found in the Accessories product category).PN / Wetted parts of pumping block<ul><li>ACCH000X / Hastelloy</li><li>ACCP000X /<span style="-webkit-text-stroke-width:0px;display:inline !important;float:none;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;"> PEEK</li><li>ACC0000X / Stainless steel</li><li>ACCT000X / Titanium</li></ul><h4>Parameters:</h4><ul><li>Flow rate: 0.1 – 100 ml/min</li><li>Maximum operating pressure: 30 MPa (4351 psi)</li><li>Flow rate setting: 0.001 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 0,5 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Communication: RS232, Ethernet (LAN), USB, Remote digital I/O</li><li>Power supply: 100 240V 50/60Hz 250VA</li><li>Dimensions W x H x D: 280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li>Weight: 12 (26,5 lb)</li><li>Output capillary outer diameter: 1/16”</li><li>Upper pressure limit (MPa): 1– 30 (4351PSI)</li><li>Precision of pressure measurement: ± 2%</li><li>Supported Gradient Box: ECB2005, ECB2005PC</li><li>Display, keypad: yes&nbsp;</li><li>Pressure Compensation Learning: yes</li></ul></div>

ECOM ECP2300 Preparative Pump

<div style="display:block;" aria-labelledby="tab_item-0">Powerful and durable pumps suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. An extended&nbsp;number of wetted material versions – not only stainless steel but also Hastelloy, titanium, and PEEK.&nbsp;ECP2300 are designed with 2 pump heads connected in parallel with a piston diameter of 3/8”.Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. The pumps can operate in two modes, i.e. using constant pressure or using the newly added option of constant flow rate.<h4>Gradient function:&nbsp;</h4>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes ECB2005, and ECB2005PC are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.Customers can use the pistons‘ back-washing function, after working, for example, with a buffered mobile phase.<h4>Seals:&nbsp;</h4>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.<h4>Software support:&nbsp;</h4>The pumps are supported by ECOMAC and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a>.<h3>Features and Benefits</h3><ul><li>All models include a display and keyboard for manual control</li><li>Precise low-pressure gradient mixing (when connected with a gradient module)</li><li>Very good durability and reliability with proper maintenance</li><li>Extended number of wetted material versions – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li>Piston back-washing function for work with buffered or aggressive mobile phases</li><li>New learning algorithm for pulsation suppression</li><li>Integrated leakage sensor</li><li>Custom pump modifications of request</li></ul>This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).Service videos -Seals replacement and&nbsp;Valve replacement :&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.ecomsro.com/support/service-videos">https://www.ecomsro.com/support/service-videos</a><h4>Parameters:</h4><ul><li>Flow rate: 0.001 – 300 ml/min</li><li>Maximum operating pressure: 15 MPa (2176 psi)</li><li>Flow rate setting: 0.001 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 0.5 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Communication: RS232,Ethernet (LAN),USB,Remote digital I/O</li><li>Power supply: 100 240V 50/60Hz 200VA</li><li>Dimensions W x H x D: 280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li>Weight: 13 kg (28.7 lb)</li><li>Output capillary outer diameter: 1/8”</li><li>Upper pressure limit (MPa): 1– 15 (2176PSI)</li><li>Precision of pressure measurement: ± 2%</li><li>Supported Gradient Box: ECB2005, ECB2005PC</li><li>Pressure Compensation Learning: yes&nbsp;</li></ul></div>

ECOM Separtrix PP03 Preparative Pump

Pulse-less triplex piston pumps designed for preparative chromatography applications, equipped with unique compact three-piston head for smooth flow and easy maintenance.

Separtrix PP03 are pulse-less triplex piston pumps designed for preparative chromatography applications - nevertheless, they can be used everywhere, where high pressure, precise flow without pulsations, and high resistance against corrosion are needed. Pumps are equipped with unique compact three-piston head for smooth flow and easy maintenance without a risk of leakage on connecting parts. Back flushed pistons are covered by a hard and chemically resistant polycrystalic carbon layer. Piston cylinders have low dead volume and samples injected through the pump appear low spreading.Pumps with different flow rate ranges differ only in head type, piston diameters, and tubing diameters. Separtrix PP03 are usually supplied with an asynchronous motor and frequency converter but are also available with a step motor which allows the use of the pump in an extremely broad range of flow rates - both for analytical and preparative applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECOM_Separtrix_PP_03_Preparative_Pump_4_6079b99e0f.jpg" alt="ECOM: Separtrix PP03 Preparative Pump" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECOM_Separtrix_PP_03_Preparative_Pump_4_6079b99e0f.jpg 245w," sizes="100vw" width="1349" height="503">Special configurations of Separtrix PP03 pumps are used for three phases low pressure gradient – in this case is extremely important that these pumps offer a nearly constant flow rate not only on the output but on the input too. Low-pressure gradient units are delivered with three solenoid valves on the plate which can be connected to the pump side. The power supply for valves is installed inside the pump box. 10 linear steps can be programmed.Separtrix PP03 pumps are delivered in configuration for high-pressure binary gradient as well. Two pumps – master and slave types – are working together. The master pump controls the flow rate and gradient composition (10 linear steps), slave pump is delivered without a control display, keyboard(s), and pressure sensor. All Separtrix PP03 pumps are equipped with keyboard(s) and graphic display. There is a possibility to set flow rate, pressure limit, and flow correction. A second keyboard is included for gradient programming in the case of low-pressure gradient pumps and high-pressure gradient master pumps.Separtrix PP 03 can be used in the mode of remote control. Pumps can cooperate with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity data station (Dataapex)</a>. Flow rate, gradient composition, and pressure limit can be programmed. The control module for PP 03 pumps was successfully tested and agreed upon by Dataapex. PP 03 pumps can be controlled using Ecom software ECOMAC as well.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECOM_Separtrix_PP_03_Preparative_Pump_2_ce1c6f0f8f.jpg" alt="ECOM: Separtrix PP03 Preparative Pump" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECOM_Separtrix_PP_03_Preparative_Pump_2_ce1c6f0f8f.jpg 245w," sizes="100vw" width="1377" height="832"><h3>Types &amp; Description with technical parameters</h3>SEPARTRIX PP03 A: Piston pulsless pump, three pistons O.D. 10 mm with a diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor vector frequencyconverter, flow rate 25 ml/min. – 800 ml/min., 260 bar (3500 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 B: Piston pulsless pump, three pistons O.D. 14 mm with diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor with vector frequency converter, flow rate 50 ml/min. – 1600 ml/min., 160 bar (2100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 A: Piston pulsless pump, three pistons O.D. 10 mm with a diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor vector frequencyconverter, flow rate 25 ml/min. – 800 ml/min., 260 bar (3500 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 B: Piston pulsless pump, three pistons O.D. 14 mm with diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor with vector frequency converter, flow rate 50 ml/min. – 1600 ml/min., 160 bar (2100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 C: Piston pulsless pump, three pistons O.D. 20 mm with diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor with vector frequency converter, flow rate 100 ml/min. – 3200 ml/min., 80 bar (1100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 AG: PP03 A pump, flow rate 25 ml/min. – 800 ml/min., max. 260 bar (3500 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder + 3 x valve power supply in pump unit), second keyboard for gradient control&nbsp;SEPARTRIX PP03 BG: PP03 B pump, three phases, flow rate 50 ml/min. – 1600 ml/min., max. 150 bar (2100 PSI), low-pressure gradient included (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03 C: Piston pulsless pump, three pistons O.D. 20 mm with diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor with vector frequency converter, flow rate 100 ml/min. – 3200 ml/min., 80 bar (1100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 AG: PP03 A pump, flow rate 25 ml/min. – 800 ml/min., max. 260 bar (3500 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder + 3 x valve power supply in pump unit), second keyboard for gradient control&nbsp;SEPARTRIX PP03 BG: PP03 B pump, three phases, flow rate 50 ml/min. – 1600 ml/min., max. 150 bar (2100 PSI), low-pressure gradient included (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03 CG: PP03 C pump, flow rate 100 ml/min. – 3200 ml/min., 80 bar (1000 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03S A: Piston pulsless pump, three pistons O.D 10 mm with diamond layer in one stainless steel head, ceramic ball valves, step motor, flow rate 1 ml/min. – 400 ml/min., 250 bar (3500 PSI), pressure measurement and limitSEPARTRIX PP03S B: Piston pulsless pump, three pistons O.D 14 mm with diamond layer in one stainless steel head, ceramic ball valves, step motor, flow rate 1 ml/min. – 800 ml/min., 150 bar (2100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03S C: Piston pulsless pump, three pistons O.D 20 mm with diamond layer in one stainless steel head, ceramic ball valves, step motor, flow rate 2 ml/min. – 1600 ml/min., 70 bar (800 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03S AG: PP03S A pump, flow rate 1 ml/min. – 400 ml/min., 250 bar (3500 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03S BG: PP03S B pump, flow rate 1 ml/min. – 800 ml/min., 150 bar (2100 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03S CG: PP03S C pump, flow rate 2 ml/min. – 1600 ml/min., 70 bar (800 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03SM A: PP03S A pump, flow rate 0,5 ml/min. – 400 ml/min., 250 bar (3500 PSI), master pump for binary high-pressure gradient, second keyboard for gradient programming&nbsp;SEPARTRIX PP03SM B: PP03S B pump, flow rate 1 ml/min. – 800 ml/min., 150 bar (2100 PSI), master pump for binary high-pressure gradient, second keyboard for gradient programmingSEPARTRIX PP03SM C: PP03S C pump, flow rate 2 ml/min. – 1600 ml/min., 70 bar (800 PSI), master pump for binary high-pressure gradient, second keyboard for gradient programmingSEPARTRIX PP03SS A: PP03SS A pump, flow rate 0,5 ml/min. – 400 ml/min., 250 bar (3500 PSI), slave pump for binary gradient, no display, no keyboard&nbsp;SEPARTRIX PP03SS B: PP03SS B pump, flow rate 1 ml/min. – 800 ml/min., 150 bar (2100 PSI), slave pump for binary gradient, no display, no keyboardSEPARTRIX PP03SS C: PP03SS C pump, flow rate 2 ml/min. – 1600 ml/min., 70 bar (800 PSI), slave pump for binary gradient, no display, no keyboard

ECOM Separchrom PC01-HPLC Industrial Preparative Columns with Axial Compression

Separchrom PC01 ECOM industrial preparative columns correspond to the highest requirements of preparative liquid chromatography. They are designed to be used in a mode of axial compression with a moving input piston. 

<div style="display:block;" aria-labelledby="tab_item-0">HPLC Columns for prep chromatography. High performance columns with axial compression.Separchrom PC01 ECOM columns correspond to the highest requirements of preparative liquid chromatography. They are designed to be used in a mode of axial compression with a moving input piston. In the original design, they are equipped with identical pistons on input and output (biaxial compression as an option). In the ECOM Separchrom PC FE modification, they have a fixed end plate on the output instead of the piston. Pistons and plates are equipped with frits. A liquid distributing system is installed under each frit. The design guarantees perfect piston flow, high efficiency even for high injection volume, and simple accessibility of frits for cleaning. Columns can be packed by dynamic slurry method or by sedimentation or with sorbent in a dry state.SEPARCHROM PC 01 ECOM columns are made of stainless steel AISI 316L (tube inner surface is polished to reach Ra &lt; 0.3 µm) with stainless steel pistons and UHMWPE (PTFE on the request) sealing rings. The stroke of the main piston is proportional to the used hydraulic cylinder or flange bolt length. Pistons are sectional; the frit (sintered mesh disc 3 µm pore size, 5 mm thickness) with the frit ring can be removed. The output plate in the FE version is made of UHMWPE, frit is fixed with a simple frit ring. Column flanges are not welded, but screwed onto the column tube. No temperature increase during manufacturing means no change in steel properties. The column stand made of stainless steel profiles is included for columns over 100 mm I.D.Hydraulic cylinders fitted on the upper flange are offered with manual oil pumps as well as with fully automated electric motor-driven devices for column packing/unpacking procedures.<h3>Accessories</h3>Columns are offered with a broad scale of accessories such as filling adapters, slurry mixing vessels, slurry transporting pumps, etc.<h3>COLUMN CONFIGURATION EXAMPLES</h3>Columns are custom-made, do not hesitate to ask for any other sizes within the possible ranges<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_1_1795482301.jpg" alt="ECOM: Separchrom PC01-HPLC Column - available configuration - part 1" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_1_1795482301.jpg 236w," sizes="100vw" width="1440" height="952"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_2_6d3198af67.jpg" alt="ECOM: Separchrom PC01-HPLC Column - available configuration - part 2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_2_6d3198af67.jpg 239w," sizes="100vw" width="1442" height="942"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_3_9fd77a1054.jpg" alt="ECOM: Separchrom PC01-HPLC Column - available configuration - part 3" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_01_HPLC_Column_available_configuration_part_3_9fd77a1054.jpg 245w," sizes="100vw" width="1435" height="315"></div>

ECOM Separchrom PC02-MPLC (Medium-pressure) Columns for Prep Chromatography

Industrial-scale Preparative Columns PC02 - Columns for medium-pressure with axial bed compression for prep chromatography.

<div style="display:block;" aria-labelledby="tab_item-0">ECOM Separchrom PC02 medium-pressure stainless steel columns are intended for separation where high pressure is not necessary, but good efficiency can still be expected. They are made of stainless steel tubes.Part of the column is an input piston made of ultra-high molecular weight polyethylene and it is equipped with stainless steel support and stainless steel mesh frit. Flow distributor with groves is incorporated into the piston to guarantee piston flow with nearly zero pressure drop. The design guarantees simple accessibility of frits for cleaning.Columns can be packed by dynamic slurry method, or by sedimentation, or with sorbent in the dry state, both with silica and polymer sorbents. For soft polymer sorbents, ECOM Separchrom PC02 oil systems can be equipped with a special software application to compensate for the swelling and unswelling of sorbents in different mobile phases (the column piston is moved in very small steps up and down during use).<h3>Materials</h3>Medium pressure prep chromatography column ECOM SEPARCHROM PC 02 is made of AISI 316L stainless steel, UHMWE moving pistons are equipped with integral sealing wings. Frits (stainless steel mesh 5 µm pore size, 5 mm thickness) can be fixed with a stainless steel ring. The stand made of stainless steel profiles is included.<h3>COLUMN CONFIGURATION EXAMPLES</h3>Columns are custom-made, do not hesitate to ask for any other sizes within the possible ranges<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_02_HPLC_Column_available_configuration_part_1_dfb61faf03.jpg" alt="ECOM: Separchrom PC02-HPLC Column - available configurations - part 1" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_02_HPLC_Column_available_configuration_part_1_dfb61faf03.jpg 245w," sizes="100vw" width="1641" height="939"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_02_HPLC_Column_available_configuration_part_2_cb9f807757.jpg" alt="ECOM: Separchrom PC02-HPLC Column - available configurations - part 2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_02_HPLC_Column_available_configuration_part_2_cb9f807757.jpg 245w," sizes="100vw" width="1645" height="364"></div>

ECOM Separchrom PC04-Non-metal Columns for Prep Chromatography

Industrial-scale preparative non-metal columns for prep chromatography with axial compression correspond to the highest requirements of preparative liquid chromatography. 

<div style="display:block;" aria-labelledby="tab_item-0">Polyethylene cartridge columns are usually intended for flash chromatography. We offer powerful ECOM Separchrom PC04 columns made of high-quality ultra-high molecular weight polyethylene, equipped with moving pistons and with no metal in contact with the mobile phase. Non-metal columns are required in biochromatography and some other special applications.Plastic columns for preparative liquid chromatography ECOM SEPARCHROM PC 04, are unfilled, and made of ultra-high molecular weight polyethylene. They are equipped with the same pistons on the input and the output. The input piston is moved either by flange bolts (type A), or by a central stainless steel threaded rod (type B). The pistons are fitted with plastic polypropylene frits 10 µm and a distribution system.<h3>COLUMN CONFIGURATION EXAMPLES</h3>Columns are custom-made, do not hesitate to ask for any other sizes within the possible ranges<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_Separchrom_PC_04_HPLC_Column_available_configurations_8767eb40ae.jpg" alt="ECOM: Separchrom PC04-HPLC Column - available configurations" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_Separchrom_PC_04_HPLC_Column_available_configurations_8767eb40ae.jpg 245w," sizes="100vw" width="1645" height="823"></div>

Products focused on PrepLC distributed by ECOM spol. s r.o. - page 2

Related content

Webinars LabRulezLCMS Week 27/2026

Online Deglycosylation of Monomeric Intact Proteins Using the PNGase Rc Immobilized-Enzyme Reactor

PFAS in Water: Why We’re Missing 96% of the Problem