<div><div><div><p><strong>Scientists at IOCB Prague have developed a breakthrough technology that streamlines aptamer development. The new method integrates several biochemical and bioinformatics steps and paves the way for new diagnostic and therapeutic applications. Ongoing applications include wearable sensors that monitor selected biomarkers in real time and provide early warnings of impending health complications. The findings were published in </strong><i><strong>Nature Communications</strong></i><strong>.</strong></p></div></div><div><p>Aptamers are short DNA or RNA strands that can recognize and bind to a specific target molecule with high precision. Similar to antibodies, they can be used to detect these molecules or modulate their activity. Unlike antibodies, they are much more stable, can be produced synthetically, and can be chemically modified to achieve the desired properties. As a result, they can offer capabilities that cannot be achieved with antibodies.</p></div><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg" alt="IOCHB Prague: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Chemically modified DNA aptamer bound to the target protein." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg 228w," sizes="100vw" width="1343" height="916"></p><p>As demand grows for accurate and rapid diagnostic tools, aptamers are often better suited to these applications than antibodies. However, developing aptamers is both experimentally demanding and time-consuming. A team of scientists from IOCB Prague, led by Dr. Marek Ondruš and <a target="_blank" rel="noopener noreferrer" href="https://hocek.group.uochb.cz/en"><strong>Prof. Michal Hocek</strong></a>, has now developed a technology that significantly shortens the development process.</p><p><i>“Developing an aptamer is often like looking for a needle in a haystack. Among trillions of different DNA sequences, we search for the one that binds strongly and specifically to a target molecule. Our new approach first screen for ‘aptamer families’ – hundreds of related sequences – and then identify the best-performing member of the family. This approach can reduce development time from several months to only a few days,” </i>says Marek Ondruš.</p><blockquote><p><strong>Video:</strong><a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=_3zXUeVMugU"> New technology streamlines the development of aptamers, synthetic alternatives to antibodies&nbsp;</a></p></blockquote><p>The team has also expanded the potential applications of aptamers by chemically modifying their building blocks. For example, introducing functional groups similar to those found naturally in amino acids allows the resulting molecules to combine the advantages of both DNA and antibodies: Their sequence is encoded by the order of nucleotides in DNA, while the attached chemical modifications greatly expand their ability to recognize biologically relevant targets and mimic interactions between proteins that are underlying many biological processes.</p><p>The researchers demonstrated the potential of this new method using the human insulin receptor, a key protein that regulates blood sugar levels. They developed an aptamer that specifically binds to the receptor and elucidated the molecular basis of this interaction using cryo-electron microscopy. <i>“We have shown that chemically introduced functional groups play a crucial role not only in target recognition and formation of the aptamer–protein complex, but also in stabilizing the DNA aptamer’s structure. Our findings provide new insights into how chemically modified nucleic acids can mimic key properties of proteins,”</i> says Marek Ondruš.</p><p>In the future, the technology could complement – or, in some applications, even replace – antibody-based diagnostic tools. Researchers are collaborating with <a target="_blank" rel="noopener noreferrer" href="http://iocbtech.cz/">IOCB Tech</a> on its further development, as the company helps to bring discoveries from IOCB Prague into practical applications.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg" alt="IOCHB Prague/Tomáš Belloň: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Dr. Marek Ondruš, first author of the study, and Prof. Michal Hocek, head of the Bioorganic and Medicinal Chemistry of Nucleic Acids research group at IOCB Prague." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg 234w," sizes="100vw" width="2000" height="1335"></p><blockquote><h4><strong>Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-73676-y#citeas"><strong>Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries</strong></a></h4><p>ablo Alberto Franco-Urquijo, Marek Ondruš, Jaroslav Kurfürst, Jana Škerlová, Irena Selicharová, Lucie Mužíková Čechová, Hana Šváchová, Alena Semerádtová, Anatolij Filimoněnko, Adéla Fejfarová, Jiří Homola, Tomáš Kouba &amp; Michal Hocek</p><p><i><strong>Nat. Commun., 2026</strong></i></p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41467-026-73676-y"><span>https://doi.org/10.1038/s41467-026-73676-y</span></a><span>&nbsp;</span></p><h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>Discovery of functional nucleic acids from randomized libraries typically relies on multiple, time-consuming iterative rounds of in vitro selection with low success rate. Here, we present a single-round selection strategy for rapid screening of multiple over-represented nucleobase-modified DNA libraries and various selection conditions, capable of identifying high-affinity modified aptamers. Double partition followed by amplification of eluted sequences, NGS analysis and clustering provides fast identification of aptamer candidates. Screening of modified DNA libraries containing modified adenine and uracil nucleotides bearing hydrophobic aromatic phenyl and indole moieties results in development of an aptamer binding human insulin receptor with sub-nanomolar affinity and exquisite specificity. Cryo-EM structure reveals the importance of each aromatic modification, either in stabilizing the secondary structure or facilitating interactions with the protein surface. This approach addresses the main drawbacks of aptamer selection and has potential for high-throughput screening and accelerating the development of next-generation aptamers for diagnostics or therapeutics.</p></div>

New technology streamlines the development of aptamers, synthetic alternatives to antibodies

<p><strong>Clarity 10.2 expands instrument control capabilities with new control modules and updates the MS extension to help you work more efficiently.</strong></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity</strong></a><strong> 10.2 highlights</strong></h4><ul><li>New and updated control modules</li><li>Improved MS data import and export</li><li>Obsolete hardware support ended</li></ul><h4><strong>Instrument control modules</strong></h4><p>Clarity 10.2 introduces new instrument control modules from Annuo (AS-3117, FAS-3823) and updated instrument controls form Sykam and Ecom.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Instrument_control_modules_5de26782b0.jpg" alt="DataApex: Instrument control modules" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Instrument_control_modules_5de26782b0.jpg 228w," sizes="100vw" width="380" height="260"></p><h4><strong>MS data import/export improvements</strong></h4><p>Clarity improves MS data exchange, especially for *.cdf files. MS instruments now support export and import of multiple spectral data and non-spectral signal data in the same CDF file. Import also better handles uniform sampling information, m/z Step values, scale factors, and file names with non-ASCII characters. Exporting individual chromatogram signals from MS instruments is now available through a separate Export Chromatogram dialog.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg" alt="DataApex: MS data import-export improvements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg 228w," sizes="100vw" width="378" height="259"></p><h4><strong>Obsolete hardware support terminated</strong></h4><p>Due to changes in Windows driver security requirements, this update discontinues support for obsolete hardware, including INT7, INT9, U-PAD, U-PAD2, and CB20.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg" alt="DataApex: Obsolete hardware support terminated" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg 228w," sizes="100vw" width="378" height="259"></p><blockquote><p><strong>Explore the complete list of all changes, fixes, and additions in version 10.2.</strong></p><p><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1"><strong>See the complete changelog&nbsp;</strong></a></p></blockquote>

Clarity Chromatography Software 10.2 released!

<p><strong>🎤Dr. Leigh Terry (University of Alabama)</strong></p><p><strong>In this episode of Concentrating on Chromatography, we sit down with Dr. Leigh Terry from the University of Alabama to explore one of the biggest challenges in environmental analysis today: understanding—and accurately measuring—PFAS in water systems.</strong></p><p>While most regulatory methods focus on a small number of compounds like PFOA and PFOS, there may be thousands of PFAS compounds in the environment—and much of that burden goes undetected.</p><h4><strong>In this episode, we cover:</strong></h4><ul><li>Why PFAS are called “forever chemicals” (and why that label is evolving)</li><li>The limitations of targeted<strong> LC-MS/MS methods</strong></li><li>What the fluorine mass balance problem reveals about PFAS destruction</li><li>How combustion ion chromatography (<strong>CIC</strong>) helps measure total organofluorine</li><li>Why “99.99% removal” doesn’t always mean what you think</li><li>Where PFAS actually end up in wastewater treatment systems</li><li>The most effective technologies for PFAS removal today</li><li>Why drinking water may only account for ~20% of total PFAS exposure</li></ul><h4><strong>Key Insight:</strong></h4><p>Even when analyzing dozens of PFAS compounds, researchers may only be capturing a small fraction of total organic fluorine—raising major questions about how we monitor and regulate these contaminants.</p><h3><strong>Video Transcription</strong></h3><p>In this episode of <i>Concentrating on Chromatography</i>, Dr. Leigh Terry discusses PFAS contamination in drinking water, the analytical challenges associated with measuring these compounds, current treatment technologies, and future directions for monitoring and regulation. The conversation highlights how analytical chemistry, environmental engineering, and public health intersect in addressing one of today's most discussed classes of emerging contaminants.</p><h4><strong>Understanding PFAS and Their Significance</strong></h4><h5><strong>The University of Alabama Water Quality Research Center</strong></h5><p>Dr. Leigh Terry is part of the University of Alabama's Center for Water Quality Research. The center operates an analytical laboratory focused on drinking water quality and the detection of contaminants at extremely low concentrations.</p><p>The laboratory specializes in:</p><ul><li>Drinking water analysis</li><li>Organic contaminants</li><li>Emerging contaminants</li><li>PFAS monitoring</li><li>Water quality research</li></ul><p>Their work supports both research initiatives and practical solutions for water utilities and environmental agencies.</p><h4><strong>Why Focus on PFAS?</strong></h4><p>Although PFAS compounds have existed since the 1940s, public awareness has increased dramatically only in recent years.</p><p>According to Dr. Terry, several factors contributed to the rapid growth of PFAS research:</p><ul><li>Increased media attention</li><li>New regulatory initiatives</li><li>Growing evidence of human exposure</li><li>Expanding research opportunities</li><li>Greater understanding of potential health risks</li></ul><p>PFAS are not new contaminants. Rather, society is only beginning to understand their prevalence in the environment and their potential impacts on human health.</p><h4><strong>The Development of Standardized PFAS Methods</strong></h4><p>One major advancement in PFAS analysis has been the development of standardized EPA methods.</p><p>These methods allow:</p><ul><li>Consistent testing procedures</li><li>Comparable results between laboratories</li><li>Regulatory monitoring</li><li>Reliable data interpretation</li></ul><p>Dr. Terry notes that many of these standardized approaches began appearing around 2018–2019, providing a common framework for analytical laboratories and researchers.</p><h4><strong>How Large Is the PFAS Universe?</strong></h4><p>Many people are familiar with PFOA and PFOS, two of the most heavily studied PFAS compounds. However, these represent only a small fraction of the broader PFAS family.</p><p>PFOA and PFOS are often referred to as:</p><ul><li>Legacy PFAS compounds</li><li>Original PFAS contaminants</li><li>Early commercial PFAS chemicals</li></ul><p>The challenge is that even minor modifications to molecular structure create entirely new PFAS compounds.</p><p>Researchers estimate:</p><ul><li>Thousands of PFAS compounds exist</li><li>Some estimates exceed 10,000 compounds</li><li>Exact numbers depend on how PFAS are defined</li></ul><p>Scientific debate continues regarding classification and definition, making precise counting difficult.</p><h4><strong>Why PFAS Are Called "Forever Chemicals"</strong></h4><p>Dr. Terry notes that the phrase "forever chemicals" has become widely used, although she suggests the terminology could benefit from rebranding.</p><p>PFAS persistence results from two key chemical properties.</p><h5><strong>1. Amphiphilic Structure</strong></h5><p>PFAS molecules contain:</p><ul><li>A water-loving (hydrophilic) head group</li><li>A water-repelling (hydrophobic) fluorinated tail</li></ul><p>This unusual combination allows PFAS to partition between:</p><ul><li>Water</li><li>Solids</li><li>Air-water interfaces</li><li>Foam</li></ul><p>Unlike many organic compounds, PFAS can interact effectively with multiple environmental phases.</p><h5><strong>2. Extremely Strong Carbon–Fluorine Bonds</strong></h5><p>The carbon–fluorine bond is among the strongest bonds in organic chemistry.</p><p>As Dr. Terry explains:</p><ul><li>Large amounts of energy are required to break these bonds</li><li>Natural degradation processes are often ineffective</li><li>Destruction can require highly aggressive treatment conditions</li></ul><p>These properties make PFAS extremely persistent in both environmental systems and biological organisms.</p><h4><strong>Toxicity, Regulations, and Environmental Behavior</strong></h4><h5><strong>Understanding PFAS Toxicity</strong></h5><p>One of the biggest challenges in PFAS regulation is the incomplete understanding of toxicity.</p><p>Dr. Terry explains that EPA regulations generally consider three factors:</p><ul><li>Presence in drinking water sources</li><li>Toxicity to humans</li><li>Availability of practical treatment options</li></ul><p>While some PFAS compounds are known to be harmful, many others remain insufficiently studied. Furthermore, PFAS often occur as mixtures rather than individual compounds.</p><p>This creates important scientific questions:</p><ul><li>What is the toxicity of individual PFAS?</li><li>What is the toxicity of PFAS mixtures?</li><li>How should cumulative exposure be evaluated?</li></ul><p>These uncertainties continue to drive active research.</p><h4><strong>What Happens to PFAS in Wastewater Treatment Plants?</strong></h4><p>PFAS do not behave like many conventional contaminants.</p><p>Rather than remaining fully dissolved in water, they often accumulate at interfaces such as:</p><ul><li>Water-solid interfaces</li><li>Water-air interfaces</li><li>Foam layers</li><li>Biological solids</li></ul><p>As a result:</p><ul><li>Some PFAS accumulate in biosolids</li><li>Some partition into foam</li><li>Some remain in treated effluent</li></ul><p>Their distribution depends on wastewater composition and treatment processes.</p><h4><strong>Regulatory Monitoring and Targeted LC-MS Analysis</strong></h4><p>Current EPA regulations focus on targeted analysis of specific PFAS compounds.</p><p>Examples include:</p><ul><li>PFOA</li><li>PFOS</li></ul><p>Drinking water regulations currently establish maximum contaminant levels (MCLs) for these compounds at extremely low concentrations.</p><p>Advantages of targeted LC-MS analysis include:</p><ul><li>Clearly defined analytes</li><li>Established regulatory limits</li><li>High analytical sensitivity</li><li>Reliable quantitation</li></ul><p>However, limitations remain:</p><ul><li>Only a small subset of PFAS is measured</li><li>Many PFAS compounds remain undetected</li><li>Total PFAS burden may be underestimated</li></ul><p>As Dr. Terry notes, targeted analysis provides only part of the overall picture.</p><h4><strong>The Fluorine Mass Balance Problem</strong></h4><p>One of the most important concepts discussed in the interview is fluorine mass balance.</p><p>When PFAS degrade, fluorinated fragments may remain in the environment.</p><p>This creates a critical question:</p><p>Are treatment technologies truly destroying PFAS, or merely transforming them into shorter-chain PFAS compounds?</p><p>To answer this, researchers perform fluorine mass balance calculations using:</p><ul><li>Targeted PFAS analysis</li><li>Fluoride measurements</li><li>Total organic fluorine measurements</li></ul><p>A successful destruction process should ultimately convert PFAS into fluoride ions rather than simply producing smaller PFAS molecules.</p><h4><strong>Advanced Analysis, Treatment Technologies, and Future Directions</strong></h4><h5><strong>Combining Targeted and Bulk Fluorine Measurements</strong></h5><p>Dr. Terry's group complements targeted LC-MS analysis with measurements of total organic fluorine.</p><p>Approaches include:</p><ul><li>Absorbable Organic Fluorine (AOF)</li><li>Extractable Organic Fluorine (EOF)</li><li>Combustion Ion Chromatography (CIC)</li></ul><p>In these methods:</p><ul><li>Organic fluorine is captured</li><li>Samples are combusted</li><li>Released fluoride is quantified</li></ul><p>This provides insight into total fluorinated content, although contributions may originate from sources beyond PFAS, including pharmaceuticals and consumer products.</p><h4><strong>Surprising Findings from Combined Analysis</strong></h4><p>One important finding from wastewater studies was that targeted PFAS measurements accounted for only a small fraction of total organic fluorine.</p><p>Dr. Terry reports that:</p><ul><li>Approximately 4% of measured AOF could be explained by targeted PFAS compounds</li><li>The majority of organic fluorine remained unidentified</li></ul><p>This suggests that environmental fluorine chemistry is considerably more complex than currently represented by routine monitoring programs.</p><h4><strong>Preventing PFAS Contamination in the Laboratory</strong></h4><p>PFAS analysis requires exceptionally strict quality control.</p><p>Dr. Terry's laboratory routinely:</p><ul><li>Tests every new batch of SPE cartridges</li><li>Tests carbon tubes before use</li><li>Uses DI water blanks</li><li>Verifies consumables labeled as PFAS-free</li><li>Collects field blanks</li><li>Monitors analytical blanks throughout workflows</li></ul><p>These practices help prevent contamination from laboratory materials and consumables, a common challenge in ultra-trace PFAS analysis.</p><h4><strong>Current PFAS Treatment Technologies</strong></h4><p>Three technologies currently dominate PFAS removal in drinking water treatment:</p><ul><li>Granular Activated Carbon (GAC)</li><li>Ion Exchange Resins</li><li>Reverse Osmosis (RO)</li></ul><p>Selection depends on:</p><ul><li>PFAS concentration</li><li>Water quality</li><li>Local operating conditions</li><li>Utility infrastructure</li></ul><p>Although many emerging technologies are being investigated, these remain the most widely implemented solutions.</p><h4><strong>Membrane Filtration and Pretreatment</strong></h4><p>Dr. Terry collaborates with colleagues studying membrane-based PFAS treatment.</p><p>Her own work focuses on pretreatment through biological filtration.</p><p>The strategy involves:</p><ul><li>Removing natural organic matter</li><li>Reducing membrane fouling</li><li>Improving membrane performance</li><li>Extending operational efficiency</li></ul><p>This integrated approach demonstrates how multiple treatment technologies can work together to improve PFAS removal.</p><h4><strong>Regional Monitoring Projects</strong></h4><p>The University of Alabama team participates in EPA-supported monitoring efforts along the Gulf Coast.</p><p>Their observations indicate:</p><ul><li>PFAS rarely occur alone</li><li>Multiple PFAS compounds frequently co-occur</li><li>Contamination often originates from identifiable point sources</li></ul><p>Examples include:</p><ul><li>Industrial facilities</li><li>Manufacturing operations</li><li>Military installations</li><li>Carpet manufacturing facilities</li></ul><p>Source identification remains a critical component of environmental monitoring programs.</p><h4><strong>Future Directions for PFAS Monitoring</strong></h4><p>Looking ahead, Dr. Terry sees several opportunities for improvement.</p><p>Desired advances include:</p><ul><li>In situ monitoring technologies</li><li>Portable detection systems</li><li>Reduced analytical complexity</li><li>Lower instrument costs</li><li>Less expensive consumables</li><li>Faster analysis workflows</li></ul><p>Current PFAS testing requires substantial expertise, specialized instrumentation, and rigorous quality control, making widespread implementation challenging.</p><h4><strong>Dr. Terry's Perspective on Long-Term Solutions</strong></h4><p>When asked what major PFAS challenge she would most like to solve, Dr. Terry offered a broader perspective.</p><p>Rather than focusing solely on remediation, she emphasized prevention.</p><p>Her view is that society should:</p><ul><li>Better evaluate chemical toxicity before commercialization</li><li>Assess risks before large-scale deployment</li><li>Reduce introduction of harmful compounds into the environment</li><li>Support utilities tasked with contaminant removal</li></ul><p>She notes that water utilities often bear the responsibility of removing contaminants they did not create, highlighting the need for broader societal and regulatory support.</p><h4><strong>Final Thoughts</strong></h4><p>Dr. Terry encourages consumers to:</p><ul><li>Learn where their drinking water originates</li><li>Read annual water quality reports</li><li>Stay informed about local water quality</li><li>Consider appropriate home filtration where needed</li></ul><p>At the same time, she emphasizes confidence in municipal water systems and the importance of continued investment in water quality research, monitoring, and treatment technologies.</p><h4><strong>Conclusion</strong></h4><p>PFAS remain one of the most complex challenges in environmental chemistry and water treatment. Through advanced analytical methods such as LC-MS/MS, combustion ion chromatography, fluorine mass balance approaches, and large-scale monitoring programs, researchers like Dr. Leigh Terry are helping improve our understanding of these persistent contaminants.</p><p>The interview highlights that solving the PFAS challenge will require a combination of better analytical tools, improved treatment technologies, stronger regulatory frameworks, and a greater emphasis on prevention before contaminants enter the environment.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

PFAS in Water: Why We’re Missing 96% of the Problem

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Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

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The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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Waters ACQUITY UPLC C18 Columns

ACQUITY UPLC columns offer unmatched speed, resolution, and peak shape for a wide range of analytes. With advanced particle technologies and broad pH stability, they support efficient, reproducible separations in both routine and demanding applications.

<h5><strong>ACQUITY UPLC BEH C18 Columns</strong></h5><ul><li>Universal C18 column</li><li>Suitable for a diverse range of analytes</li><li>High efficiency</li><li>Industry-leading mobile phase pH 1-12 and temperature compatibility</li></ul><h5><strong>ACQUITY UPLC CSH C18 Columns</strong></h5><ul><li>Universal column choice for UV and MS applications</li><li>Suitable for a broad range of compounds</li><li>Alternate selectivity to BEH C18</li><li>Exceptional peak shape and increased load capacity</li><li>Suited for basic compounds at low pH</li></ul><h5><strong>ACQUITY UPLC HSS T3 Columns</strong></h5><ul><li>Ideal for the enhanced retention of polar and non-polar compounds</li><li>Compatible with 100% aqueous mobile phases</li><li>Mechanically stable at elevated pressures</li></ul><h5><strong>ACQUITY UPLC HSS C18 Columns</strong></h5><ul><li>General purpose silica-based C18 chemistry</li><li>Suitable for a broad range of compounds</li><li>Provides exceptional peak shapes and low pH stability</li><li>Delivers increased retention compared to hybrid organic/inorganic C18 columns</li></ul><h5><strong>ACQUITY UPLC HSS C18 SB Columns</strong></h5><ul><li>Low coverage silica-based C18 chemistry</li><li>Provides alternate selectivity for compounds impacted by silanophilic interactions</li><li>Increased silanol activity results in greater retention of basic compounds while reducing the retention of non-basic analytes</li><li>Provides exceptional peak shape and imparts Selectivity for Bases at low pH (SB)</li></ul><h4><strong>ACQUITY UPLC Application-Specific Column Characteristics</strong></h4><h5><strong>ACQUITY UPLC Peptide BEH C18 Columns</strong></h5><ul><li>Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH</li><li>Outstanding peak capacity and peak shape in TFA or FA when compared to 100% silica based C18 columns</li><li>Two pore sizes (130 Å and 300 Å) for a wide range of peptides</li></ul><h5><strong>ACQUITY UPLC Peptide CSH C18 Columns</strong></h5><ul><li>Outstanding peak capacities with formic acid for MS-based applications</li><li>Excellent performance with TFA for optical based applications</li><li>Less dependence on TFA or FA to achieve optimal peak capacity</li><li>Accepts greater peptide mass loads than many competitive technologies for detection of low-level impurities</li><li>At low pH with the positive charge on CSH, you get less secondary interactions with the particle</li></ul><h5><strong>ACQUITY UPLC Peptide HSS T3 Columns</strong></h5><ul><li>Separations that require silica-based selectivity</li><li>Separations that necessitate increased peptide retention</li><li>Ideally suited for small, hydrophilic peptides<br>&nbsp;</li></ul><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Waters ACQUITY UPLC BEH C8 Column

The Waters ACQUITY BEH C8 sorbent is less retentive compared to the ACQUITY BEH C18 sorbent. 

<p><strong>The ACQUITY BEH C8</strong> sorbent is <strong>less retentive compared to the ACQUITY BEH C18 </strong>sorbent. A method developer may prefer the speed offered by a less retentive column, while improving peak shape and chromatographic performance. The <strong>trifunctionally bonded BEH particles</strong> offer the widest usable <strong>pH range (1-12)</strong>, <strong>superior low pH stability</strong>, and <strong>ultra-low column bleed</strong>.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C8</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Hybrid</li><li><strong>pH Range Min</strong>: 1 pH</li><li><strong>pH Range Max: </strong>12 pH</li><li><strong>Maximum Pressure: </strong>18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity: </strong>Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>130 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>USP Classification:</strong> L7</li><li><strong>Carbon Load: </strong>13 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg" alt="Waters: ACQUITY UPLC BEH C8 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Waters ACQUITY UPLC Protein BEH C4 Column

The Waters ACQUITY UPLC Protein BEH C4 column is QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point. 

<p><strong>The ACQUITY UPLC Protein BEH C4 </strong>column is QC tested and optimized to <strong>separate proteins</strong> based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the <strong>C4 ligand is less retentive </strong>and will <strong>minimize protein carryover,</strong> <strong>increase protein recovery</strong>, and <strong>improve peak capacity</strong>.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C4</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Hybrid</li><li><strong>pH Range Min</strong>: 2 pH</li><li><strong>pH Range Max: </strong>10 pH</li><li><strong>Endcapped: </strong>No</li><li><strong>Silanol Activity: </strong>Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>300 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>USP Classification:</strong> L26</li><li><strong>Carbon Load: </strong>8 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg" alt="Waters: Kolona ACQUITY UPLC BEH C8" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Waters ACQUITY UPLC HSS CN Column

Waters ACQUITY UPLC HSS CN chemistry is an ultra-performance general purpose propyl cyano bonded phase that provides superior peak shape for bases, increased retention, and extremely long column lifetimes at low pH.

<p><strong>ACQUITY UPLC HSS CN</strong> chemistry is an ultra-performance general purpose <strong>propyl cyano bonded phase</strong> that provides <strong>superior peak shape</strong> <strong>for bases</strong>, <strong>increased retention</strong>, and <strong>extremely long column lifetimes at low pH</strong>. ACQUITY HSS CN columns are fully scalable and transferable to HPLC separations.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> Cyno (CN)</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min</strong>: 2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Endcapped: </strong>No</li><li><strong>Silanol Activity: </strong>High</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> HSS</li><li><strong>USP Classification:</strong> L10</li><li><strong>Carbon Load: </strong>5 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg" alt="Waters: ACQUITY UPLC HSS CN Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Waters ACQUITY UPLC BEH Phenyl Column

The Waters ACQUITY BEH Phenyl columns provide complementary selectivity to C18 stationary phases, especially for polyaromatic compounds. 

<p><strong>ACQUITY BEH Phenyl </strong>columns provide <strong>complementary selectivity to C18</strong> stationary phases, <strong>especially for polyaromatic compounds</strong>. This sorbent, along with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1828"><strong>XBridge BEH Phenyl</strong></a>, provide you with the most chemiscally stable phenyl sorbent on the market and provides you with ultra-low column bleed, long column lifetimes and excellent peak shape.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> Phenyl</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Hybrid</li><li><strong>pH Range Min</strong>: 1 pH</li><li><strong>pH Range Max: </strong>12 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity: </strong>Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>130 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>USP Classification:</strong> L11</li><li><strong>Carbon Load: </strong>15 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg" alt="Waters: ACQUITY UPLC BEH Phenyl Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Waters ACQUITY Advanced Polymer Chromatography (APC) Calibration Standards Kits

Eliminate manual preparation of custom standard calibration mixes with ACQUITY Advanced Polymer Chromatography (APC) Calibration Standards.

<div><div><h3><strong>Frequent calibration for greater confidence in your results</strong></h3></div></div><div><div><p><strong>Eliminate manual preparation of custom standard calibration mixes with ACQUITY Advanced Polymer Chromatography (APC) Calibration Standards, which provide the right number of data points for your targeted molecular weight (MW) range. ACQUITY APC Calibration Standards are specifically designed to match the molecular weight range of the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1950"><strong>ACQUITY APC Columns</strong></a><strong>.</strong></p><p>ACQUITY APC polymethylmethacrylate and polystyrene standards are configured into <strong>Low</strong>, <strong>Middle</strong>, and <strong>High Molecular Weight Calibration Kits</strong>, as well as a convenient<strong> Method Development Kit </strong>that includes the full separation range of all three kits combined. These chromatography standards are designed to provide a maximum range of narrow standard data points for a specific column set or molar mass range.</p><p>With ACQUITY APC Calibration Standards, you can conduct calibration with<strong> 3-5x shorter cycle times</strong>, enabling you to run more replicates and more easily include calibration with each test set. With increased calibration frequency, you can have higher confidence in the accuracy of each test run.</p><div><div><ul><li><strong>Simple and easy</strong>&nbsp;to use by adding solvent and injecting</li><li><strong>Shorter preparation time</strong> resulting in faster calibration cycle time</li><li><strong>Greater confidence</strong> in molecular weight determination of samples</li><li><strong>Reduce variances</strong> in analysis providing increased product quality</li></ul><div><div><p><strong>Recommended Use:&nbsp;</strong>For <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/194"><strong>advanced polymer chromatography </strong></a>calibration.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Advanced_Polymer_Chromatography_APC_Calibration_Standards_Kits_2_1e32010bc1.jpg" alt="Waters ACQUITY Advanced Polymer Chromatography (APC) Calibration Standards Kits: The ACQUITY Advanced Polymer Chromatography (APC) Calibration Standards are available in polystyrene and polymethylmethacrylate configured into Low, Middle, and High Molecular Weight Calibration Kits. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Advanced_Polymer_Chromatography_APC_Calibration_Standards_Kits_2_1e32010bc1.jpg 245w," sizes="100vw" width="1024" height="565"></p></div></div></div></div></div></div>

Waters CORTECS HILIC HPLC Columns

Waters CORTECS HILIC Columns are high-effiency columns based on a solid-core particle that are designed to retain extremely polar analytes. 

<p><strong>CORTECS HILIC Columns </strong>are high-effiency columns based on a solid-core particle that are designed to <strong>retain extremely polar analytes</strong>. They offer <strong>orthogonal selectivity vs. C18</strong> columns.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> HILIC</li><li><strong>Separation Mode:</strong> Hydrophilic Interaction (HILIC)</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min</strong>: 1 pH</li><li><strong>pH Range Max:</strong> 5 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>No</li><li><strong>Silanol Activity: </strong>High</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>90 Å</li><li><strong>System: </strong>HPLC</li><li><strong>Particle Technology:</strong> Solid core</li><li><strong>USP Classification:</strong> L3</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CORTECS_C8_Column_68f0f00415.jpg" alt="Waters: CORTECS HILIC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CORTECS_C8_Column_68f0f00415.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>CORTECS Columns</strong></h3><h4><strong>Solid-core columns that live up to their potential.</strong></h4><p>Your lab needs to develop methods for challenging separations even faster. Increase resolution for even your toughest samples with the enhanced column efficiency of <strong>CORTECS solid-core columns</strong>, which provide the flexibility and power you need to rapidly separate a wide array of compounds.</p><p><strong>CORTECS Columns</strong> are available in <strong>reversed-phase and HILIC options</strong> across <strong>seven column chemistries</strong> with <strong>different particle characteristics</strong> and innovative charged surface modifications for 20% faster separations and 25% more resolution at low operating back pressures.</p><h4><strong>Overview</strong></h4><ul><li>Maximize efficiency with <strong>high-performance columns</strong> created using state-of-the-art column packing techniques, bonding and end-capping processes.</li><li>Achieve <strong>greater throughput and speed</strong> than fully porous particle columns.</li><li>Get <strong>simple and seamless transfer</strong> with consistent results across different particle sizes and column configurations.</li><li><strong>Flexibly develop methods for challenging separations</strong> faster with reversed-phase and HILIC options across seven column chemistries.</li><li><strong>Prolong column lifetimes</strong> with optional VanGuard Cartridges and pre-columns specifically designed using CORTECS stationary phases.</li><li><strong>Recommended Use:</strong> To maximize resolution and speed for all LC separations.</li></ul><h4><strong>CORTECS Column Characteristics</strong></h4><h5><strong>CORTECS C18+ Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>A positively charged surface delivers excellent peak shape for basic compounds at low pH.</li></ul><h5><strong>CORTECS C18 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Balanced retention of acids, bases, and neutrals at low and mid-range pH.</li></ul><h5><strong>CORTECS C8 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Similar selectivity but shorter retentivity when compared to C18 columns.</li></ul><h5><strong>CORTECS T3 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Enables to use of 100% aqueous mobile phase and increased retention of polar compounds.</li></ul><h5><strong>CORTECS Phenyl Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for polyaromatic compounds.</li></ul><h5><strong>CORTECS Shield RP18 Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for phenolic compounds.</li></ul><h5><strong>CORTECS HILIC Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>HILIC Column</li><li>Offers orthogonal selectivity when compared to reversed-phase columns.</li></ul><h4><strong>Reliable columns. Consistent performance.</strong></h4><p>Get consistent batch-to-batch performance year after year with reliable CORTECS Columns.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg" alt="Waters: Consistent performance" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg 227w," sizes="100vw" width="1024" height="704"></p><h4><strong>Method transfer just got easier.</strong></h4><p>Get the versatility you need for seamless method transfer, with consistent results and fully scalable particle sizes for HPLC, UHPLC, and UPLC platforms.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg" alt="Waters: Modernize and speed up your existing methods." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg 191w," sizes="100vw" width="1024" height="837"></p><h4><strong>Increased resolution and increased throughput.</strong></h4><p>Maximize column efficiency and throughput with increased resolution for the toughest samples on any LC system.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Maximize_Column_Efficiency_69800e15cb.jpg" alt="Waters: Maximize Column Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Maximize_Column_Efficiency_69800e15cb.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters CORTECS T3 HPLC Columns

Waters CORTECS T3 columns are compatible with 100% aqueous mobile phases, and provide excellent retention for polar and non-polar analytes.

<p><strong>CORTECS T3 columns </strong>are <strong>compatible with 100% aqueous mobile phases</strong>, and provide <strong>excellent retention for polar and non-polar analytes.</strong> The particle morphology offers high efficnecy and lower backpressure when compared to fully porouse particles of similar particle size. <strong>CORTECS T3</strong> provides you with <strong>ultra-low column MS bleed</strong>, <strong>long lifetime</strong>s and <strong>excellent peak shape</strong>.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode:</strong> Reversed phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min</strong>: 2 pH</li><li><strong>pH Range Max:</strong> 8 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>120 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> Solid core</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon load:</strong> 4.7 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CORTECS_C8_Column_68f0f00415.jpg" alt="Waters: CORTECS T3 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CORTECS_C8_Column_68f0f00415.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>CORTECS Columns</strong></h3><h4><strong>Solid-core columns that live up to their potential.</strong></h4><p>Your lab needs to develop methods for challenging separations even faster. Increase resolution for even your toughest samples with the enhanced column efficiency of <strong>CORTECS solid-core columns</strong>, which provide the flexibility and power you need to rapidly separate a wide array of compounds.</p><p><strong>CORTECS Columns</strong> are available in <strong>reversed-phase and HILIC options</strong> across <strong>seven column chemistries</strong> with <strong>different particle characteristics</strong> and innovative charged surface modifications for 20% faster separations and 25% more resolution at low operating back pressures.</p><h4><strong>Overview</strong></h4><ul><li>Maximize efficiency with <strong>high-performance columns</strong> created using state-of-the-art column packing techniques, bonding and end-capping processes.</li><li>Achieve <strong>greater throughput and speed</strong> than fully porous particle columns.</li><li>Get <strong>simple and seamless transfer</strong> with consistent results across different particle sizes and column configurations.</li><li><strong>Flexibly develop methods for challenging separations</strong> faster with reversed-phase and HILIC options across seven column chemistries.</li><li><strong>Prolong column lifetimes</strong> with optional VanGuard Cartridges and pre-columns specifically designed using CORTECS stationary phases.</li><li><strong>Recommended Use:</strong> To maximize resolution and speed for all LC separations.</li></ul><h4><strong>CORTECS Column Characteristics</strong></h4><h5><strong>CORTECS C18+ Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>A positively charged surface delivers excellent peak shape for basic compounds at low pH.</li></ul><h5><strong>CORTECS C18 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Balanced retention of acids, bases, and neutrals at low and mid-range pH.</li></ul><h5><strong>CORTECS C8 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Similar selectivity but shorter retentivity when compared to C18 columns.</li></ul><h5><strong>CORTECS T3 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Enables to use of 100% aqueous mobile phase and increased retention of polar compounds.</li></ul><h5><strong>CORTECS Phenyl Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for polyaromatic compounds.</li></ul><h5><strong>CORTECS Shield RP18 Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for phenolic compounds.</li></ul><h5><strong>CORTECS HILIC Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>HILIC Column</li><li>Offers orthogonal selectivity when compared to reversed-phase columns.</li></ul><h4><strong>Reliable columns. Consistent performance.</strong></h4><p>Get consistent batch-to-batch performance year after year with reliable CORTECS Columns.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg" alt="Waters: Consistent performance" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg 227w," sizes="100vw" width="1024" height="704"></p><h4><strong>Method transfer just got easier.</strong></h4><p>Get the versatility you need for seamless method transfer, with consistent results and fully scalable particle sizes for HPLC, UHPLC, and UPLC platforms.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg" alt="Waters: Modernize and speed up your existing methods." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg 191w," sizes="100vw" width="1024" height="837"></p><h4><strong>Increased resolution and increased throughput.</strong></h4><p>Maximize column efficiency and throughput with increased resolution for the toughest samples on any LC system.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Maximize_Column_Efficiency_69800e15cb.jpg" alt="Waters: Maximize Column Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Maximize_Column_Efficiency_69800e15cb.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters CORTECS Shield RP18 HPLC Columns

Waters CORTECS Shield RP18 columns provides complimentary selectivity to C8 and C18 stationary phases. The embedded carbamate group in the bonded phase ligand provides alternate selectivity, especially for phenolic compounds.

<p><strong>CORTECS Shield RP18</strong> columns provides <strong>complimentary selectivity to C8 </strong>and <strong>C18</strong> stationary phases. The <strong>embedded carbamate group</strong> in the bonded phase ligand provides <strong>alternate selectivity, especially for phenolic compounds</strong> compared to straight chain alkyl columns. The solid core particle morphology offers high efficiency and lower backpressures when compared to fully porouse particles of similar particle size.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode:</strong> Reversed phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min</strong>: 2 pH</li><li><strong>pH Range Max:</strong> 8 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>90 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> Solid core</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon load:</strong> 6.4 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CORTECS_C8_Column_68f0f00415.jpg" alt="Waters: CORTECS Shield RP18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CORTECS_C8_Column_68f0f00415.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>CORTECS Columns</strong></h3><h4><strong>Solid-core columns that live up to their potential.</strong></h4><p>Your lab needs to develop methods for challenging separations even faster. Increase resolution for even your toughest samples with the enhanced column efficiency of <strong>CORTECS solid-core columns</strong>, which provide the flexibility and power you need to rapidly separate a wide array of compounds.</p><p><strong>CORTECS Columns</strong> are available in <strong>reversed-phase and HILIC options</strong> across <strong>seven column chemistries</strong> with <strong>different particle characteristics</strong> and innovative charged surface modifications for 20% faster separations and 25% more resolution at low operating back pressures.</p><h4><strong>Overview</strong></h4><ul><li>Maximize efficiency with <strong>high-performance columns</strong> created using state-of-the-art column packing techniques, bonding and end-capping processes.</li><li>Achieve <strong>greater throughput and speed</strong> than fully porous particle columns.</li><li>Get <strong>simple and seamless transfer</strong> with consistent results across different particle sizes and column configurations.</li><li><strong>Flexibly develop methods for challenging separations</strong> faster with reversed-phase and HILIC options across seven column chemistries.</li><li><strong>Prolong column lifetimes</strong> with optional VanGuard Cartridges and pre-columns specifically designed using CORTECS stationary phases.</li><li><strong>Recommended Use:</strong> To maximize resolution and speed for all LC separations.</li></ul><h4><strong>CORTECS Column Characteristics</strong></h4><h5><strong>CORTECS C18+ Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>A positively charged surface delivers excellent peak shape for basic compounds at low pH.</li></ul><h5><strong>CORTECS C18 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Balanced retention of acids, bases, and neutrals at low and mid-range pH.</li></ul><h5><strong>CORTECS C8 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Similar selectivity but shorter retentivity when compared to C18 columns.</li></ul><h5><strong>CORTECS T3 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Enables to use of 100% aqueous mobile phase and increased retention of polar compounds.</li></ul><h5><strong>CORTECS Phenyl Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for polyaromatic compounds.</li></ul><h5><strong>CORTECS Shield RP18 Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for phenolic compounds.</li></ul><h5><strong>CORTECS HILIC Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>HILIC Column</li><li>Offers orthogonal selectivity when compared to reversed-phase columns.</li></ul><h4><strong>Reliable columns. Consistent performance.</strong></h4><p>Get consistent batch-to-batch performance year after year with reliable CORTECS Columns.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg" alt="Waters: Consistent performance" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg 227w," sizes="100vw" width="1024" height="704"></p><h4><strong>Method transfer just got easier.</strong></h4><p>Get the versatility you need for seamless method transfer, with consistent results and fully scalable particle sizes for HPLC, UHPLC, and UPLC platforms.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg" alt="Waters: Modernize and speed up your existing methods." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg 191w," sizes="100vw" width="1024" height="837"></p><h4><strong>Increased resolution and increased throughput.</strong></h4><p>Maximize column efficiency and throughput with increased resolution for the toughest samples on any LC system.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Maximize_Column_Efficiency_69800e15cb.jpg" alt="Waters: Maximize Column Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Maximize_Column_Efficiency_69800e15cb.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters CORTECS C18+ HPLC Columns

Waters CORTECS C18+ columns are general purpose, charged surface, high efficiency C18 columns based on a solid-core particle.

<p><strong>CORTECS C18+</strong> columns are general purpose, charged surface, high efficiency C18 columns based on a solid-core particle that is designed to deliver excellent peak shape for basic analytes at low pH.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode:</strong> Reversed phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min</strong>: 2 pH</li><li><strong>pH Range Max:</strong> 8 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>90 Å</li><li><strong>System: </strong>HPLC</li><li><strong>Particle Technology:</strong> Solid core</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon load:</strong> 5.7 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CORTECS_C8_Column_68f0f00415.jpg" alt="Waters: CORTECS C18+ Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CORTECS_C8_Column_68f0f00415.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>CORTECS Columns</strong></h3><h4><strong>Solid-core columns that live up to their potential.</strong></h4><p>Your lab needs to develop methods for challenging separations even faster. Increase resolution for even your toughest samples with the enhanced column efficiency of <strong>CORTECS solid-core columns</strong>, which provide the flexibility and power you need to rapidly separate a wide array of compounds.</p><p><strong>CORTECS Columns</strong> are available in <strong>reversed-phase and HILIC options</strong> across <strong>seven column chemistries</strong> with <strong>different particle characteristics</strong> and innovative charged surface modifications for 20% faster separations and 25% more resolution at low operating back pressures.</p><h4><strong>Overview</strong></h4><ul><li>Maximize efficiency with <strong>high-performance columns</strong> created using state-of-the-art column packing techniques, bonding and end-capping processes.</li><li>Achieve <strong>greater throughput and speed</strong> than fully porous particle columns.</li><li>Get <strong>simple and seamless transfer</strong> with consistent results across different particle sizes and column configurations.</li><li><strong>Flexibly develop methods for challenging separations</strong> faster with reversed-phase and HILIC options across seven column chemistries.</li><li><strong>Prolong column lifetimes</strong> with optional VanGuard Cartridges and pre-columns specifically designed using CORTECS stationary phases.</li><li><strong>Recommended Use:</strong> To maximize resolution and speed for all LC separations.</li></ul><h4><strong>CORTECS Column Characteristics</strong></h4><h5><strong>CORTECS C18+ Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>A positively charged surface delivers excellent peak shape for basic compounds at low pH.</li></ul><h5><strong>CORTECS C18 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Balanced retention of acids, bases, and neutrals at low and mid-range pH.</li></ul><h5><strong>CORTECS C8 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Similar selectivity but shorter retentivity when compared to C18 columns.</li></ul><h5><strong>CORTECS T3 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Enables to use of 100% aqueous mobile phase and increased retention of polar compounds.</li></ul><h5><strong>CORTECS Phenyl Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for polyaromatic compounds.</li></ul><h5><strong>CORTECS Shield RP18 Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for phenolic compounds.</li></ul><h5><strong>CORTECS HILIC Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>HILIC Column</li><li>Offers orthogonal selectivity when compared to reversed-phase columns.</li></ul><h4><strong>Reliable columns. Consistent performance.</strong></h4><p>Get consistent batch-to-batch performance year after year with reliable CORTECS Columns.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg" alt="Waters: Consistent performance" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg 227w," sizes="100vw" width="1024" height="704"></p><h4><strong>Method transfer just got easier.</strong></h4><p>Get the versatility you need for seamless method transfer, with consistent results and fully scalable particle sizes for HPLC, UHPLC, and UPLC platforms.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg" alt="Waters: Modernize and speed up your existing methods." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg 191w," sizes="100vw" width="1024" height="837"></p><h4><strong>Increased resolution and increased throughput.</strong></h4><p>Maximize column efficiency and throughput with increased resolution for the toughest samples on any LC system.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Maximize_Column_Efficiency_69800e15cb.jpg" alt="Waters: Maximize Column Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Maximize_Column_Efficiency_69800e15cb.jpg 234w," sizes="100vw" width="1024" height="682"></p>

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