In the week of February 16, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars">👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</a></h4>👉 Search and filter in the largest global database with over 5 000 GC, LC, MS and spectroscopy webinars.</blockquote><h5>1. Agilent Technologies: From Complex to Simplicity – Get More Peace of Mind on Your HPLC Workflows with InfinityLab Assist</h5><ul><li>Mo, 16.2.2026 11:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5908">Registration</a></li></ul>Discover how Agilent InfinityLab Assist simplifies LC system control with intuitive multitouch operation, guided troubleshooting, and smart monitoring to boost efficiency and reduce downtime.<h5>2. Thermo Fisher Scientific: Driving the Process Development of Recombinant Therapeutic Products with Automated MS-Based Target Peptide Mapping</h5><ul><li>Tu, 17.2.2026 11:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5931">Registration</a></li></ul>Explore automated MS-based MAM workflows for peptide mapping, enabling faster CMC decisions and scalable analytics from development to QC readiness.<h5>3. Thermo Fisher Scientific: PFAS Analysis Solutions – Innovations for Today’s Labs</h5><ul><li>Tu, 17.2.2026 11:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5818">Registration</a></li></ul>Explore the latest challenges and solutions in PFAS analysis for environmental and food testing, including advanced workflows that improve sensitivity, accuracy, and lab efficiency.<h5>4. Phenomenex: Understanding and Applying USP Chapter 621 for Generic Pharmaceutical Applications</h5><ul><li>We, 18.2.2026 10:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5794">Registration</a></li></ul>Learn the key guidelines defined in USP &lt;621&gt; and practical examples of how to apply them in generic pharmaceutical method development.<h5>5. SCIEX: Genome Integrity Analysis of Adeno-Associated Viruses (AAV) by Genome Sizing Using a SCIEX Capillary Electrophoresis System</h5><ul><li>We, 18.2.2026 11:30 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5935">Registration</a></li></ul>Join this webinar to learn how capillary electrophoresis enables reliable genome integrity analysis of AAV for gene therapy applications.<h5>6. Waters Corporation: The Gold Standard – Achieving Optimal Peptide Purification with Reversed-Phase Chromatography</h5><ul><li>We, 18.2.2026 17:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5917">Registration</a></li></ul>Discover why reversed-phase chromatography remains the gold standard for peptide purification and analytical performance.<h5>7. Axcend: Understanding Capillary LC – Part 5: Detectors, Sensitivity, and MS/UV</h5><ul><li>We, 18.2.2026 20:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5829">Registration</a></li></ul>Join experts to explore MS, UV, and electrochemical detectors at the capillary scale and learn how to achieve reliable sensitivity in capillary LC workflows.<h5>8. Thermo Fisher Scientific: Single Cell and Immunopeptidomics Explorations on Orbitrap Astral MS</h5><ul><li>Th, 19.2.2026 11:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5932">Registration</a></li></ul>Discover how Orbitrap Astral MS and Vanquish Neo HPLC enable deep single-cell proteomics and immunopeptidomics from ultra-low-input clinical samples.<h5>9. Evosep Biosystems: Experimental Design Considerations for Scalable Proteomics</h5><ul><li>Th, 19.2.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5836">Registration</a></li></ul>Explore best practices for experimental design in LC-MS–based proteomics to build robust, reproducible, and scalable analytical workflows.<h5>10. Waters Corporation: Ask Me Anything About Managing Computerized System Risks to Data Integrity</h5><ul><li>Th, 19.2.2026 17:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5809">Registration</a></li></ul>Learn practical strategies to manage computerized system risks in regulated laboratories and protect data integrity while ensuring compliance.<h5>11. Shimadzu Corporation: Automating a Comprehensive Amino Acid Panel for Inborn Errors of Metabolism</h5><ul><li>Th, 19.2.2026 20:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5936">Registration</a></li></ul>Discover how Shimadzu’s CLAM-2040 automates LC-MS/MS sample preparation to standardize workflows, reduce manual handling, and improve consistency in clinical labs.

Webinars LabRulezLCMS Week 08/2026

Imaging of Protein Assemblies up to 231 kDa in Tissues with Nano-DESI Mass Spectrometry

Metalloproteins play essential roles in marine phytoplankton, yet their metalloproteomes remain poorly characterized. This study combines protein modeling, quantitative proteomics, and ICP-MS measurements to assess metal requirements across several phytoplankton species, including chlorophytes, a haptophyte, and a cyanobacterium.Results show strong agreement between proteome-inferred metal demands and measured cellular metal quotas, while providing deeper insight into biological functions. Mg-containing proteins were most abundant overall, with Zn, Fe, and Mn as dominant trace cofactors. Distinct differences between prokaryotic and eukaryotic phytoplankton were observed, and strain-level analysis demonstrated how metalloproteomes reflect environmental adaptation and metal limitation strategies.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233">Unlocking Phytoplankton Metallomes with Comparative Analysis of Metal Quotas, Quantitative Proteomics, and Inferred Metalloproteomes</a></h4>Qiong Zhang*, Jiayou Ge, Fengjie Liu, Shabaz Mohammed, Kedong Yin, and Rosalind E. M. RickabyEnviron. Sci. Technol. 2025, 59, 50, 27367–27377<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.est.5c11233">https://doi.org/10.1021/acs.est.5c11233</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.Many important biological processes of phytoplankton, including photosynthesis, carbon fixation, and nitrogen reduction, rely on enzymes that have metals as cofactors. <a href="javascript:void(0);">(1)</a> It has been estimated that half of all enzymes must be associated with a particular metal to function. <a href="javascript:void(0);">(2)</a> The availability of metals in the environment has changed over geological time and, given imperfections in the selectivity of metal transport systems, proteins have evolved to make the best use of metals that are more available. <a href="javascript:void(0);">(3−5)</a> Depending on their evolutionary histories, different groups of marine phytoplankton may have differences in their metalloprotein compositions and metal transport strategies. The evolving trace metal availability in the environment, thus, may provide a selective pressure to promote the proliferation of different phytoplankton groups in the oceanic environment, affecting the phytoplankton community structure with knock-on effects on the marine food web and carbon sequestration. <a href="javascript:void(0);">(5)</a> It is crucial to understand the utilization of trace metals by different groups of marine phytoplankton that inhabit contrasting niches in the modern ocean, not only by assessing the accumulation of trace metals in cells but also examining the functions of trace metals in different biological pathways.In previous studies, the utilization or requirements of trace metals by marine phytoplankton were mostly determined by measuring the elemental stoichiometry in cells (metallome or quota of trace metals). <a href="javascript:void(0);">(6−8)</a> In the field, attempts have also been made to investigate the elements in phytoplankton <a href="javascript:void(0);">(9,10)</a> which have then been compared with profiles in the abiotic environment to understand their relationships. <a href="javascript:void(0);">(11)</a> The discovery of various metal storage proteins and the plasticity in elemental quotas under different environmental conditions raise the question of whether intracellular trace metal quotas reflect true differences in trace metal usage by phytoplankton. <a href="javascript:void(0);">(12,13)</a> In the past few decades, more and more studies have focused on metal–protein partnerships, using approaches such as genome-wide analysis to identify homologues of known metalloproteins and other deduced metal-binding motifs encoded within sequenced genomes. <a href="javascript:void(0);">(5,14)</a> However, this approach has limitations in estimating metal abundance in cells because not all genes predicted in the genome would be translated and expressed as proteins under all environments, and a single gene for a metalloprotein could be highly expressed, such that a translation from genetic presence to a required concentration is not linear and could be environment-dependent. To obtain in situ metal abundance in metalloproteins in cells, native separation of proteins by HPLC or PAGE followed by the detection of metals using ICP-MS <a href="javascript:void(0);">(15,16)</a> has been used. This approach also has limitations in that, without bioinformatics, it cannot identify the function of proteins within which the metals are found, and it may also depend on the composition of the media to which the organism has been exposed and in which it has been growing. An ideal way to solve the problem would be to separate and purify each of the proteins and then identify both the associated metal (e.g., by ICP-MS) and the protein sequence (e.g., by mass spectrometry). However, this approach is constrained by the resolution of chromatographic or electrophoretic techniques, making it challenging to establish the metal–protein associations unambiguously; many proteins often co-occur in metal-containing fractions. Additionally, this method requires huge volumes of biomass and is both time-consuming and complex, complicating the reconstruction of the expressed metalloproteome in cells. The techniques and challenges for metalloproteomics have been discussed in detail in previously published review articles (and the references therein). <a href="javascript:void(0);">(17)</a>In this study, we determined the trace metal utilization (the metallome) of different phytoplankton from their expressed proteome via a protein modeling and homologue identification approach, and then compared the resulting metallome with the metal quota data measured by the traditional inductively coupled plasma mass spectrometry (ICP-MS). This approach allows the building of a link between the truly used trace elements and their biological functions in phytoplankton. We aim to fill the gap between an estimation of metals encoded in the genome and metal analysis by ICP-MS in phytoplankton, which may be more broadly applicable to infer cellular metallomes.<h3>Materials and Methods</h3><h4>ICP-MS Analysis</h4>All preparation of samples for ICP-MS measurement was undertaken using trace metal clean methods. All plasticware used in this study, including culture flasks, pipette tips, and centrifuge tubes, was precleaned in 10% quartz-distilled HCl and thoroughly rinsed with 18 MΩ cm water in a clean laboratory. Trace elements in the filtered lysates of G. huxleyi (OA1, OA4, OA8, OA15, OA16, OA23, and RCC 1242) and Synechocystis (FACHB898) strains were measured using an ICP-MS. The detailed ICP-MS method and instrumental settings are described in Zhang et al. (2018). <a href="javascript:void(0);">(31)</a><h4>Protein Mass Spectrometry</h4>The peptides were resuspended in 5% formic acid and 5% DMSO prior to mass spectrometry. They were separated on an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=3&amp;search=Ultimate+3000+UHPLC">Ultimate 3000 UHPLC </a>system (Thermo Fisher Scientific) and electrosprayed directly into a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/559">Q-Exactive Mass Spectrometer</a> (Thermo Fisher Scientific) through an EASY-Spray nanoelectrospray ion source (Thermo Fisher Scientific). The peptides were trapped on a C18 PepMap100 precolumn (300 μm i.d. × 5 mm, 100 Å, Thermo Fisher Scientific) using solvent A (0.1% formic acid in water) at a pressure of 500 bar. The peptides were separated on an in-house packed analytical column (75 μm i.d. × 50 cm packed with ReproSil-Pur 120 C18-AQ, 1.9 μm, 120 Å, Dr. Maisch GmbH) using a linear gradient (length: 65 min, 15% to 35% solvent B [0.1% formic acid in acetonitrile], flow rate: 200 nL/min). The raw data were acquired on the mass spectrometer in a data-dependent mode (DDA). Full scan MS spectra were acquired in the Orbitrap (scan range 350–1500 m/z, resolution 70000, AGC target 3e6, maximum injection time 50 ms). After the MS scans, the 10 most intense peaks were selected for HCD fragmentation at 30% of normalized collision energy. HCD spectra were also acquired in the Orbitrap (resolution 17500, AGC target 5e4, maximum injection time 120 ms, isolation window 1.5 m/z) with the first fixed mass at 180 m/z. Charge exclusion was selected for unassigned and 1+ ions. The dynamic exclusion was set to 20 s.<h3>Results and Discussion</h3><h4>Trace Elements Determined from the Expressed Proteomes of Different Phytoplankton</h4>Based on the expressed proteome sequences, we predicted metal-binding proteins and compared their distribution among various phytoplankton species (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig2">Figure 2</a>). The inference revealed 28 different metals in G. huxleyi, 31 different metals in C. reinhardtii, and 30 different metals in Synechocystis. Although only 6 different metals were identified in O. tauri proteins, it has to be noted that the analysis for O. tauri was only based on the 40 most abundant proteins reported in this organism <a href="javascript:void(0);">(21)</a> and, hence, the listed elements, including Mg, Fe, Mn, Ca, Zn, and Cu, may represent the most essential metals for the species.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Environ_Sci_Technol_2025_59_50_27367_27377_Figure_2_The_number_of_metal_binding_proteins_identified_in_the_expressed_proteome_of_the_haptophyte_G_9b8ac0f004.jpeg" alt="Environ. Sci. Technol. 2025, 59, 50, 27367–27377: Figure 2. The number of metal-binding proteins identified in the expressed proteome of the haptophyte G. huxleyi (a), the chlorophyte C. reinhardtii (b) and O. tauri(c), and the cyanobacteria Synechocystis (d). Pie charts show the percentages of metalloproteins containing specific metals. Original proteome data for G. huxleyi are from Mckew et al. (2013) (24) for C. reinhardtii are from Hsieh et al. (2013) (77) for O.tauriare from Martin et al. (2012) (21) for Synechocystisare from Chen et al. (2018). (22) *Noted that for O. tauri, only the 40 most abundant proteins were included in this analysis. ND: no metal binding was found in the proteins." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Environ_Sci_Technol_2025_59_50_27367_27377_Figure_2_The_number_of_metal_binding_proteins_identified_in_the_expressed_proteome_of_the_haptophyte_G_9b8ac0f004.jpeg 123w," sizes="100vw" width="934" height="1181">The number of proteins that bind with different metals varies dramatically in phytoplankton. For metalloproteins, there are variations in the binding affinities of different metals to organic molecules. For example, the affinities of divalent metals to proteins have a tendency to follow a universal order of preference, namely the Irving-Williams series (Mg2+ and Ca2+ [weakest binding] &lt; Mn2+ &lt; Fe2+ &lt; Co2+ &lt; Ni2+ &lt; Cu2+ &gt; Zn2+). <a href="javascript:void(0);">(43)</a> Cells can simultaneously contain proteins having high-affinity-binding metals, such as Cu and Zn, and others that require uncompetitive metals, such as Mg and Ca. One of the least competitive metals for binding with biological chelators, Mg, is even found to be the most widely distributed metal in the metalloproteomes of various phytoplankton species in this study, supporting the observations of high concentrations in the protein fraction of cells (at millimolar concentrations <a href="javascript:void(0);">(31)</a>).In all phytoplankton species in our analysis, the Mg-containing proteins are found to have the highest proportion in all metalloproteins identified. About 30% of the metalloproteins in different strains of phytoplankton are found to contain Mg or depend on it for function (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig2">Figure 2</a>), suggesting the important role of Mg in phytoplankton cells. It is generally coordinated in protein complexes by ATP or ADP and has also been found to be largely distributed in the chloroplasts or thylakoids in G. huxleyi and Synechocystis. <a href="javascript:void(0);">(22,24)</a> The essential roles of Mg in plants have been reported in various studies. Mg2+ is known to be the central atom of the chlorophyll molecule, and its levels in the chloroplast regulate the activity of key photosynthetic enzymes. <a href="javascript:void(0);">(44)</a> It was reported that approximately 15–35% of total Mg is in the chloroplasts of a plant, <a href="javascript:void(0);">(45)</a> and it also serves as an osmotically active ion in the regulation of cells <a href="javascript:void(0);">(46)</a><h4>Comparison Between Metallome Measured Directly and Reconstructed from Proteins</h4>A large proportion of metals, such as Fe, Mn, and Cu, are found in membrane proteins,and may contribute significantly to total cellular quotas. <a href="javascript:void(0);">(54,55)</a> However, for method development purpose, we endeavored to remove the cell membranes from our samples to focus mostly on the analysis of intracellular metal quotas and metalloproteins in the cytosol fraction in this study. We note that our analysis includes some membrane-bound proteins likely derived from organelles or surface membranes disrupted during sonication.The derivation of cellular metal composition, as determined by two different methods, is compared in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig3">Figure 3</a>. The abundance of metal in the proteins is determined as the relative abundance of the protein multiplied by the number of metal ions needed for the specific protein. The abundance of the proteins was normalized to ensure that the total intensities from each replicate measurement for the same species were similar. Since the proteomic data used here were generated from different studies using different instruments, we cannot quantify or compare the absolute biomass of proteins used for each species. Therefore, we cannot compare the absolute trace metal abundance between species solely from metal inferences of expressed proteomes. Nevertheless, we can estimate the relative abundance of different metals in the same species. For the elements that are both measured by the ICP-MS and identified in the proteome, there are generally good correlations (r2 = 0.81 in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig3">Figure 3</a>a and r2 = 0.74 in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig3">Figure 3</a>b) between the two approaches. This indicates that the metal compositions determined from the protein modeling may be reliable and, therefore, can reflect the metal usage in different phytoplankton. This would provide a level of assurance to further investigate the metal binding associated with various proteins, thereby deepening our understanding of the roles that different metals play in phytoplankton.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Environ_Sci_Technol_2025_59_50_27367_27377_Figure_3_Relationship_between_the_abundance_of_metals_determined_by_ICP_MS_analysis_e7b259e376.jpeg" alt="Environ. Sci. Technol. 2025, 59, 50, 27367–27377: Figure 3. Relationship between the abundance of metals determined by ICP-MS analysis (normalized to C, mmol/mol C) and metal abundance determined from the metalloproteome analysis (the unit is arbitrary). To account for strain variations, the ICP-MS data for the cellular metal content of G. huxleyi are average values from 7 G. huxleyi strains isolated from different areas of the ocean, and the proteome-derived metal composition data are average values calculated from 2 of those G. huxleyi strains (OA1 and OA16) isolated previously, and data from Mckew et al., 2013. (24) The ICP-MS data for Synechocystis are from Synechocystis sp. FACHB898 measured in this study, and the proteome data are calculated from Chen et al., 2018. (22) Noted that the abundance of metals from each proteome was calculated by using the metal stoichiometry multiplied by the relative abundance of proteins, which provides only a relative relationship of different trace metals, not absolute abundance. The symbols represent the mean values, and the error bars indicate standard deviations." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Environ_Sci_Technol_2025_59_50_27367_27377_Figure_3_Relationship_between_the_abundance_of_metals_determined_by_ICP_MS_analysis_e7b259e376.jpeg 245w," sizes="100vw" width="1384" height="641">Compared to the other metals investigated in this study, Ca and Mg appear to have a much higher relative abundance in the ICP-MS analysis than in the proteomic analysis of G. huxleyi and Synechocystis (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig3">Figure 3</a>). These two elements are essential macronutrients for phytoplankton and plants. Ca is the most prominent secondary messenger that plays a crucial role in response to extracellular stimuli in all eukaryotes. Ca2+ fluxes are transported via Ca channels and transporters, such as Ca-ATPase, regulating a series of important biological functions, including mineral uptake and utilization, K+ homeostasis adjustment, and regulatory network activity in response to P or N deficiency. <a href="javascript:void(0);">(56)</a> Ca is not always incorporated into protein structures. The Ca abundance determined from the proteins in this study, therefore, is much lower than the actual Ca needed intracellularly. Similarly, the intracellular Mg2+ and K+ abundances should also be higher than those determined from the protein structures. Free Mg2+ and K+ are known to regulate cation–anion balance and serve as osmotically active ions in the regulation of cells <a href="javascript:void(0);">(46)</a> but free metal ion concentrations cannot be determined through the approach used in this study. Thus, it is not surprising to see much higher relative abundances of Ca and Mg in ICP-MS analysis than in proteomic analysis (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.est.5c11233#fig3">Figure 3</a>).

Unlocking Phytoplankton Metallomes with Comparative Analysis of Metal Quotas, Quantitative Proteomics, and Inferred Metalloproteomes

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Phenomenex Kinetex Core-Shell HILIC Preparative HPLC Columns

Kinetex HILIC is a core-shell particle with unbonded silica, providing selectivity for retaining and separating polar compounds in HILIC chromatography.

Hydrophilic Polar Selectivity and Performance!The Kinetex HILIC is a core shell based particle with a unbonded silica phase is used under hydrophilic interaction chromatography conditions to provide the selectivity needed for the retention and separation of polar compounds. This versatile column can easily upgrade existing fully porous 5 µm and 3 µm methods to shorten run time, increase sensitivity, and even provide greater resolution with much higher efficiency levels.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems</li><li>Unique Polar Retentive L3 Silica</li><li>Unbonded Silica Phase</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_Core_Shell_HILIC_HPLC_Column_9ff82b0e20.webp" alt="Phenomenex: Kinetex Core-Shell HILIC HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_Core_Shell_HILIC_HPLC_Column_9ff82b0e20.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 0 %</li><li>Particle Size (μm): 5</li><li>Recommended Use: Retention and separation of polar compounds hydrophilic interaction chromatography conditions</li></ul><h4>Alternative Polar Selectivity to a C18 &nbsp;</h4>A Core-Shell based silica phase that combines high-performance with novel polar HILIC selectivity.<h4>Increase Polar Retention</h4>Used under HILIC running conditions, this phase provides the highest polar selectivity for retention and separation of hydrophilic compounds.<blockquote>Stationary phase Kinetex Core-Shell HILIC is provided in these column types: analytical and preparative</blockquote>Axia Packed Preparative Columns<ul><li>Column length (mm): 100, 150, 250</li><li>Column ID (mm): 21.2</li><li>Particle size (µm): 5</li></ul><h3>Kinetex Core-Shell Technology Columns</h3>Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media, which can be leveraged to increase resolution, significantly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.Advantages of Kinetex HPLC Columns<ul><li>Performance gains on ANY liquid chromatography system</li><li>System-to-system and lab-to-lab method portability</li><li>Improve the productivity of older, established methods.</li></ul><h4>How Does Core-Shell Technology Work?</h4>Core-shell technology in chromatography is a revolutionary approach that enhances the efficiency of HPLC systems while minimizing costs. Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for faster analyte mass transfer between the mobile and stationary phases, as diffusion occurs only through the thin, porous shell rather than the entire particle. This minimizes band broadening, leading to higher separation efficiency and sharper peaks.The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.<h3>Kinetex Core-Shell Performance on Any LC System</h3>The 5 versatile particle sizes allow you to select the best fit for your instrument.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"><h3>Particle Chemistry</h3>Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.<ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul>Kinetex particles are characterized by an exceptionally consistent size distribution, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to enhanced separation efficiency and sharper peaks.The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"><h3>Core-shell vs Fully Porous</h3><h4>High-Efficiency‚ High-Density Particle</h4>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"><h3>Expand Your Core-Shell Selectivity Toolbox</h3>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of 11 diverse stationary phases, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.<h3>Top 5 Pros of Kinetex Core-Shell Technology</h3><ul><li>Productivity: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li>Portability The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li>Performance Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li>Phases With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li>Pressure Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Phenomenex Lux Cellulose-1 Chiral HPLC Columns

Phenomenex Lux Cellulose-1 chiral HPLC column offers reliable enantiomer separation, matching or exceeding CHIRALCEL® OD® performance across multiple modes.

This universally trusted cellulose phenyl carbamate derivative is absolutely essential to any chiral screen. Lux Cellulose-1 is a guaranteed alternative to CHIRALCEL® OD® and OD-HX. Expect equivalent or better performance when using this Lux phase.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Cellulose_1_Chiral_HPLC_Columns_f70a9f21a0.jpg" alt="Phenomenex: Lux Cellulose-1" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Cellulose_1_Chiral_HPLC_Columns_f70a9f21a0.jpg 245w," sizes="100vw" width="500" height="150"><ul><li>Separation Mode: Chiral</li><li>Phase Composition: Regenerated Cellulose (RC)</li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.</li><li>Stationary phase: Cellulose tris(3,5-dimethylphenylcarbamate)</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux Cellulose-1 columns are guaranteed to perform as well or better than the equivalent CHIRALCEL® OD® and OD-H® Chiral Technologies column. Lux phases can also provide alternative selectivity to other chiral selectors for the most challenging chiral separations. Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects.The helical structure of the cellulose creates a complex steric environment that provides stronger interactions and better chiral recognition abilities. Additionally, the selector’s constituents produce hydrogen bonding, dipole, and π-π interactions that all affect the CSPs ability to separate your enantiomers effectively.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Performance_Advantage_f239557883.jpg" alt="Phenomenex: Lux Performance Advantage" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Performance_Advantage_f239557883.jpg 182w," sizes="100vw" width="677" height="580">

Phenomenex Lux Cellulose-2 Chiral HPLC Columns

Phenomenex Lux Cellulose-2 chiral HPLC column, a halogenated cellulose phase, ensures superior enantiomer separation and is a proven alternative to CHIRALCEL® OZ®.

This first to market halogenated cellulose phenyl carbamate derivative offers unique chiral recognition abilities that complement the rest of the Lux family of columns.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomene_Lux_Cellulose_2_Chiral_HPLC_Columns_4aa21cccda.jpg" alt="Phenomenex: Lux Cellulose-2 Chiral HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomene_Lux_Cellulose_2_Chiral_HPLC_Columns_4aa21cccda.jpg 245w," sizes="100vw" width="768" height="438"><ul><li>Separation Mode: Chiral</li><li>Stationary phase: Regenerated Cellulose (RC)<ul><li>Cellulose tris(3-chloro-4-methylphenylcarbamate)</li></ul></li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux Cellulose-2 columns are guaranteed to perform as well or better than the equivalent CHIRALCEL-OZ and OZ-H® Chiral Technologies column. Lux phases can also provide alternative selectivity to other chiral selectors for the most challenging chiral separations.Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects. The helical structure of the cellulose creates a complex steric environment that provides stronger interactions and better chiral recognition abilities. Additionally, the selector’s constituents produce hydrogen bonding, dipole, and π-π interactions that all affect the CSPs ability to separate your enantiomers effectively.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Performance_Advantage_cellulose_2_bbd747a32c.jpg" alt="Phenomenex: Lux Performance Advantage" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Performance_Advantage_cellulose_2_bbd747a32c.jpg 179w," sizes="100vw" width="677" height="590">

Phenomenex Lux Cellulose-3 Chiral HPLC Columns

Phenomenex Lux Cellulose-3 chiral HPLC column provides reliable enantiomer separation, performing as well or better than CHIRALCEL® OJ® with unique chiral selectivity.

This first to market halogenated cellulose phenyl carbamate derivative offers unique chiral recognition abilities that complement the rest of the Lux family of columns.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Cellulose_3_Chiral_HPLC_Columns_2_74f7d52275.jpg" alt="Phenomenex: Lux Cellulose-3 Chiral HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Cellulose_3_Chiral_HPLC_Columns_2_74f7d52275.jpg 216w," sizes="100vw" width="903" height="650"><ul><li>Separation Mode: Chiral</li><li>Stationary phase: Regenerated Cellulose (RC)<ul><li>Cellulose tris(4-methylbenzoate)</li></ul></li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux Cellulose-3 columns are guaranteed to perform as well or better than the equivalent CHIRALCEL® OJ® and OJ-H® Chiral Technologies column. Lux phases can also provide alternative selectivity to other chiral selectors for the most challenging chiral separations. Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects.The helical structure of the cellulose creates a complex steric environment that provides stronger interactions and better chiral recognition abilities. Additionally, the selector’s constituents produce hydrogen bonding, dipole, and π π interactions that all affect the CSPs ability to separate your enantiomers effectively.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Performance_Advantage_cellulose_3_a232dc359a.jpg" alt="Phenomenex: Lux Cellulose-3 Performance Advantage" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Performance_Advantage_cellulose_3_a232dc359a.jpg 179w," sizes="100vw" width="677" height="590">

Phenomenex Lux i-Cellulose-5 Chiral HPLC Columns

Phenomenex Lux i-Cellulose-5 chiral HPLC column delivers broad enantioselectivity, strong solvent compatibility, and scalable performance for LC and SFC separations.

<h4>Your New First Choice Chiral Column!</h4>The immobilization and bonding technology used within the Lux i-Cellulose-5 promotes column stability in strong organic solvents, which affords you the ability to expand your chiral separation success with more solvent systems and separation modes.<ul><li>Wide Solvent Flexibility</li><li>Broad Enantioselectivity</li><li>Strong Solvent Compatibility</li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, immobilized with a polysaccharide stationary phase. This Immobilization provides additional strong organic solvent ruggedness so that DCM, DMSO and other related strong solvents can be used on column.</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux® i-Cellulose-5 contains achemical crosslinking between the polysaccharide and silica supports which inturn provides incredible strong solvent (THF, DCM, DMSO, MtBE, Ethyl Acetate, etc.) robustness compared to coated phases and prevents the phase from being dissolved in strong solvents.Lower pressure, all purpose particle size for analytical LC (HPLC) or SFC methods and especially for high performance preparative LC or SFC puriﬁcation work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_i_Cellulose_5_Chiral_HPLC_Columns_28bc99dbb1.jpg" alt="Phenomenex: Lux i-Cellulose-5 Chiral HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_i_Cellulose_5_Chiral_HPLC_Columns_28bc99dbb1.jpg 245w," sizes="100vw" width="1541" height="595"><h4>Dependability and Seamless Scalability</h4>Our highest standards of quality will ensure that you are fully satisfied with each and every Lux chiral column as consistent quantitation and results come with every Lux batch and column that we manufacture. With matching selectivity, the 3 μm and 5 μm i-Cellulose-5 particle sizes allow you to scale down to increase resolution or easily scale up for preparative purification work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Incredible_consistency_batch_to_batch_and_between_particle_sizes_4a8adc47b8.jpg" alt="Phenomenex: Incredible consistency batch-to-batch and between particle sizes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Incredible_consistency_batch_to_batch_and_between_particle_sizes_4a8adc47b8.jpg 103w," sizes="100vw" width="677" height="1025">

Phenomenex Lux Amylose-1 Chiral HPLC Columns

Phenomenex Lux Amylose-1 chiral HPLC column ensures reliable enantiomer separation, matching or exceeding CHIRALPAK® AD® with strong recognition and broad selectivity.

This universally trusted amylose phenylcarbamate derivative is absolutely essential to any chiral screen. Lux Amylose-1 is a guaranteed alternative to CHIRALPAK® AD®. Expect equivalent or better performance when using this Lux phase.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Amylose_1_Chiral_HPLC_Columns_a5e2773084.jpg" alt="Phenomenex: Lux Amylose-1 Chiral HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Amylose_1_Chiral_HPLC_Columns_a5e2773084.jpg 245w," sizes="100vw" width="1236" height="626"><ul><li>Stationary phase: Amylose tris(3,5-dimethylphenylcarbamate)</li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux columns are guaranteed to perform as well or better than the equivalent DAICEL® Chiral Technologies column. Lux phases can also provide alternative selectivity to other chiral selectors for the most challenging chiral separations. Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects.The helical structure of the amylose creates a complex steric environment that provides stronger interactions and better chiral recognition abilities. Additionally, the selector’s constituents produce hydrogen bonding, dipole, and π π interactions that all affect the CSPs ability to separate your enantiomers effectively.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Amylose_1_separation_c517f7e910.jpg" alt="Phenomenex: Separation comparison - Lux Amylose-1 vs. CHIRALPAK AD-H" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Amylose_1_separation_c517f7e910.jpg 179w," sizes="100vw" width="677" height="590">

Phenomenex Lux i-Amylose-3 Chiral HPLC Columns

Phenomenex Lux i-Amylose-3 chiral HPLC column offers broad enantioselectivity, strong solvent stability, and proven performance as an alternative to CHIRALPAK® IG®.

<h4>Your New First Choice Chiral Column!</h4>The immobilization and bonding technology used within the Lux i-Amylose-3 promotes column stability in strong organic solvents, which affords you the ability to expand your chiral separation success with more solvent systems and separation modes.<ul><li>Wide Solvent Flexibility</li><li>Broad Enantioselectivity</li><li>Strong Solvent Compatibility</li><li>Recommended Use: Separation of enantiomers and racemic mixtures with strong stability in strong organic solvents</li></ul><h4>Achieve Greater Chiral Separation Success</h4>The Lux i-Amylose-3, like the rest of the Lux product line, is an incredibly versatile phase that has been manufactured and developed to give you your desired results again and again. This is especially true under harsh conditions where coated phases are unable to perform.Lower pressure, all purpose particle size for analytical LC (HPLC) or SFC methods and especially for high performance preparative LC or SFC puriﬁcation work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_i_Cellulose_5_Chiral_HPLC_Columns_28bc99dbb1.jpg" alt="Phenomenex: Lux i-Amylose-3 Chiral HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_i_Cellulose_5_Chiral_HPLC_Columns_28bc99dbb1.jpg 245w," sizes="100vw" width="1541" height="595"><h4>Guaranteed Alternative to CHIRALPAK® IG®</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Guaranteed_Alternative_to_CHIRALPAK_IG_584996e87b.jpg" alt="Phenomenex: Guaranteed Alternative to CHIRALPAK® IG®" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Guaranteed_Alternative_to_CHIRALPAK_IG_584996e87b.jpg 176w," sizes="100vw" width="677" height="600">

Phenomenex Strata X-AW Solid Phase Extraction (SPE)

Strata-X-AW mixed-mode SPE sorbent enables strong retention of acids (pKa ≤5) with 100% organic wash, ensuring maximum removal of interferences for clean, reliable extractions.

Weak Anion Mixed-Mode Solid Phase Extraction (SPE)A weak anion-exchange functionalized polymeric sorbent that allows for complete retention of acidic compounds with a pKa less than 5, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_AW_SPE_cd5908db4d.jpg" alt="Phenomenex: Strata-X-AW SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_AW_SPE_cd5908db4d.jpg 245w," sizes="100vw" width="500" height="268"><ul><li>Recommended Use: Extraction of acidic compounds, particularly strong acids</li></ul><h4>Starting Method</h4><h5>For Acids with pKa ≤ 5</h5><ul><li>Column: 1 mL Methanol</li><li>Equilibrate: 1 mL Water, pH 6-7</li><li>Load: Diluted Sample pH 6-7</li><li>Wash 1: 1 mL 25 mM Ammonium Acetate Buffered, pH 6-7</li><li>Wash 2: 1 mL Methanol</li><li>Elute Any Acid: 2x 500 µL 5 % NH4OH in Methanol</li><li>Elute Weak Acids: 2x 500 µL 5 % Formic Acid in Methanol</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080">Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.<h4>Available Formats</h4><ul><li>96-Well-Plate</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>1, 3, and 6mL Tubes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>Giga Tubes (12-150mL Tubes)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080">

Phenomenex Strata X-A Solid Phase Extraction (SPE)

Strata-X-A strong anion-exchange SPE retains weak acids (pKa >2) with 100% organic wash, maximizing interference removal. Available in tubes, plates, and giga formats.

Strong Anion Mixed-Mode Solid Phase Extraction (SPE)A strong anion-exchange functionalized polymeric sorbent that allows for complete retention of weakly acidic compounds with a pKa greater than 2, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_A_SPE_e107f0b6bf.jpg" alt="Phenomenex: Strata-X-A SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_A_SPE_e107f0b6bf.jpg 245w," sizes="100vw" width="500" height="268"><ul><li>Recommended Use: Extraction of weakly acidic compounds</li></ul><h4>Starting Method</h4><h5>For Acids with pKa &gt; 2</h5><ul><li>Column: 1 mL Methanol</li><li>Equilibrate: 1 mL Water, pH 6-7</li><li>Load: Diluted Sample pH 6-7</li><li>Wash 1: 1 mL 25 mM Ammonium Acetate Buffered, pH 6-7</li><li>Wash 2: 1 mL Methanol (collect this fraction to analyze Neutral/Bases)</li><li>Elute Acid: 2x 500 µL 5 % Formic Acid in Methanol</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080">Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.<h4>Available Formats</h4><ul><li>96-Well-Plate</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>1, 3, and 6mL Tubes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>Giga Tubes (12-150mL Tubes)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080">

Phenomenex Strata X-C Solid Phase Extraction (SPE)

Strata-X-C strong cation-exchange SPE retains basic compounds (pKa ≤10.5) with 100% organic wash for maximum cleanup. Offered in tubes, plates, and giga formats.

Strong Cation Mixed-Mode Solid Phase Extraction (SPE)A strong cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pKa less than 10.5, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_C_SPE_4927d7340d.jpg" alt="Phenomenex: Strata-X-C SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_C_SPE_4927d7340d.jpg 245w," sizes="100vw" width="500" height="268"><ul><li>Recommended Use: Extraction of weakly basic compounds</li></ul><h4>Starting Method</h4><h5>For Bases with pKa ≤ 10.5</h5><ul><li>Column: 1 mL Methanol</li><li>Equilibrate: 1 mL Acidified Water</li><li>Load: Diluted Acidified Sample</li><li>Wash 1: 1 mL 0.1 N HCI in Water (collect this fraction to analyze Polar Neutrals)</li><li>Wash 2: 1 mL 0.1 N HCI in Methanol (collect this fraction to analyze Neutrals/Acids)</li><li>Elute Bases: 2x 500 µL 5 % NH4OH in Methanol</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080">Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.<h4>Available Formats</h4><ul><li>96-Well-Plate</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>1, 3, and 6mL Tubes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>Giga Tubes (12-150mL Tubes)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080">

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