Struggling to accurately measure semi-volatile analytes? Stricter temperature control with HPLC-CAD analysis just might be the solution you need.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_1_f2eefd5901.jpeg" alt="Thermo Fisher Scientific How to Get More Accurate HPLC Quantitation of Semi-Volatiles" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_1_f2eefd5901.jpeg 245w," sizes="100vw" width="1024" height="512">The charged aerosol detector (CAD) is cited as one of the most capable near-universal HPLC detectors available because the response is solely based on particle charge and fully independent of chemical structure.One caveat to know is your mobile phase components must be less volatile than the analytes in solution for detection by the CAD. And while all non-volatiles give a similar uniform response, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/liquid-chromatography-information/hplc-system-components/how-hplc-detectors-work/charged-aerosol-detection/charged-aerosol-detection-cad-performance-tips.html">the response of semi-volatiles</a> depends on volatility and consequently is less predictable.So, the question then becomes is there a way to address this performance difference and make semi-volatile detection more predictable? One approach for charged semi-volatile analytes is to add a volatile ion-pairing agent of the opposite charge in the mobile phase to force non-volatile behavior.However, this method is analyte specific and may also react with impurities in your mobile phase, leading to higher noise and poorer signal-to-noise (S/N) ratio. What’s really needed is a more global approach to improve the accuracy for measuring semi-volatiles.This approach is now achievable with Temperature Coupling Mode (TCM) in the <a target="_blank" rel="noopener noreferrer" href="https://discover-pharma.com/thermo-fisher-upgrades-charged-aerosol-detection-tech-to-improve-complex-drug-analysis/">Thermo Scientific Vanquish CAD P Series</a>.<h3>Why stricter temperature control matters for measuring semi-volatiles</h3>Before we get into the specifics, you need to understand why temperature control in the CAD is critical to quantitative accuracy, especially for semi-volatiles (Figure 1).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_2_e33cff4cac.jpeg" alt="Thermo Fisher Scientific: How to Get More Accurate HPLC Quantitation of Semi-Volatiles: Figure 1. Illustration showing how the CAD works." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_2_e33cff4cac.jpeg 245w," sizes="100vw" width="1024" height="512">The two <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/liquid-chromatography-information/hplc-system-components/how-hplc-detectors-work/charged-aerosol-detection.html">most important functional components in the CAD that guide sensitivity</a> are the evaporation tube temperature (EvapT) and the charging detection module (CDM).<ul><li>EvapT plays a crucial role in determining the performance of the detector, and changing this temperature may alter analyte “selectivity”</li><li>The CDM is where particle charge and measurement happen</li></ul>In previous CAD models, the EvapT is adjustable, but the temperature of the CDM is permanently set at 40 °C. This fixed temperature difference between the EvapT and CDM could negatively impact the measurement of semi-volatile compounds that require a lower EvapT.Adding TCM resolves this issue, by linking the EvapT and CDM temperatures, thereby improving the quantitation of semi-volatile species.<h3>Using TCM to help improve quantification of semi-volatiles, like fatty acids</h3>To demonstrate the effectiveness of this new temperature coupling feature, we anlazyed a working solution of four individual fatty acids (FAs) and compared the CAD repsonse with TCM turned on and turned off (Figure 2).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_3_da6cf5006c.jpeg" alt="Thermo Fisher Scientific: How to Get More Accurate HPLC Quantitation of Semi-Volatiles: Figure 2. The mean S/N ratio of the FAs analyzed with TCM turned on and off." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_3_da6cf5006c.jpeg 245w," sizes="100vw" width="700" height="410">Notably, we observed 46.8% increase in the S/N at the optimal EvapT of 25 °C and with the TCM switched on for lauric acid, the most semi-volatile compound!Interestingly, the observed S/N improvement matches the respective analyte volatility—lauric acid (lowest MW) shows the greatest improvement, and stearic acid (highest MW) the least.Taken together, these findings show how stricter temperature control in the CAD can allow you to extend the range and improve quantification of semi-volatiles.<h3>Extending this application to impurity analysis of polysorbates</h3>Now that we’ve seen TCM is useful for analyzing fatty acids, let’s consider more complex solutions, like polysorbates.Given the CAD is well-established for determining <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0021967324010240?via%3Dihub">polysorbate impurities</a>, and fatty acid impurities are quite abundant in polysorbates, and a logical next step is to use this technique for analyzing these solutions.Afterall, free acids in polysorbates <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/blog/analyteguru/review-of-analytical-methods-for-quantifying-amp-profiling/">may form insoluble particles</a> that can seriously compromise product quality, efficacy, and patient safety. Thus, your ability to accurately measure these acids in raw materials and finished goods is of the upmost importance.Download our paper to see how the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_003867_hplc_vanquish_cad_gradient_optimized_evap_temp_an003867_na_en_b64411eb47.pdf">Vanquish CAD P Series</a> can help your lab measure fatty acids with greater confidence today.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_4_70473e3696.jpeg" alt="Thermo Fisher Scientific: How to Get More Accurate HPLC Quantitation of Semi-Volatiles: Figure 3. Thermo Scientific Vanquish CAD P Series" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_4_70473e3696.jpeg 245w," sizes="100vw" width="700" height="410"><h3>FAQs</h3><ol><li>What are the advantages of using a charged aerosol detector for HPLC analysis?</li></ol>The <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/c/b/vanquish-charged-aerosol-detector-p-series.html">charged aerosol detector</a> (CAD) is now recognized as the most capable “universal” detector available and offers several advantages over other UHPLC-based detectors including:<ul><li>Uniform response for all non-volatile analytes (ideal for quantitation when certified standards are unavailable)</li><li>Universal response independent of chemical structure (analyte does not have to form gas phase ions or possess a chromophore)</li><li>High sensitivity at high pg to low ng on column (ideal for drug impurity analysis)</li><li>Wide linear and dynamic range (measure API and impurities in one run)</li><li>Simple calibration curves (no need to deal with complex, unpredictable sigmoidal calibration curves)</li><li>Gradient compatible (inverse gradient available to ensure consistent response throughout the gradient)</li></ul><ol start="2"><li>Why should I use CAD for measuring fatty acid impurities in polysorbates?</li></ol>Fatty acids don’t contain strong chromophores, so HPLC-CAD analysis is the <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/blog/analyteguru/cad-vs-elsd-which-hplc-detector-is-your-better-option/">ideal choice</a> since the detector response depends on particle charge and is independent of analyte structure.<ol start="3"><li>How does changing the evaporation tube temperature affect the CAD response for semi-volatiles?</li></ol>A high EvapT can <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_72806_uhplc_charged_aerosol_detection_tn72806_en_5979117651/tn-72806-uhplc-charged-aerosol-detection-tn72806-en.pdf">improve sensitivity</a> for non-volatiles by reducing noise but may negatively impact measurement of semi-volatile species. Conversely, a lower EvapT can improve the detection of semi-volatile species, but sensitivity for all analytes may be adversely impacted due to increased noise.Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#HPLC #CAD #biopharma<section><blockquote><h5>Dr. Kimberly M. Lincoln</h5>Dr. Kimberly M. Lincoln is a marketing specialist in the HPLC division at Thermo Fisher Scientific. She holds a Ph.D. in bioinorganic chemistry and a B.S. in chemistry, with professional experience in digital marketing, medical device manufacturing, and chemical synthesis. Kimberly enjoys writing educational chemical content as a guide to help customers solve analytical problems. She is passionate about living a clean lifestyle and keeping the planet healthy for future generations. In her spare time, Kimberly loves to cook, cycle, attend rock concerts, and spend time with her pit bulls.</blockquote></section>

How to Get More Accurate HPLC Quantitation of Semi-Volatiles

We are pleased to announce that a maintenance release is now available for KnowItAll 2026. Maintenance releases are an important way to introduce improvements and bug fixes between major releases to make sure users get the most out of KnowItAll.In Fall 2025, we announced the KnowItAll 2026 major release featuring a new principal component analysis tool, Trendfinder, and various advances for spectral analysis workflows. This is the first update available after that release.<h5>Updates in the 2026.1 maintenance release include:</h5><ul><li>Continued to LC-MS deconvolution improvement</li><li>Updated the DEA regulations rulebase that’s used to automatically categorize structures in databases you build in MineIt according to current Drug Enforcement Agency controlled substance regulations.</li><li>Various bug fixes</li></ul>We encourage users to update now to the most recent version of the software to ensure the best performance.<ul><li><a target="_blank" rel="noopener noreferrer" href="https://get.knowitall.com/Download?_gl=1*18h0vjr*_gcl_au*Mjk3ODY0Mzg1LjE3MTMyNzcyMDEuMTkwNDkyNDI4NS4xNzEzMzY3NDc0LjE3MTMzNjc0Nzk.">Download the most recent version here</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/whats-new-in-knowitall-2026/">What's New in KnowItAll 2026</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/knowitall-version-history/">KnowItAll version history</a></li></ul><h4>About KnowItAll</h4>KnowItAll Software offers solutions to analyze and manage multiple types of spectral and chemical data in multiple file and instrument formats. KnowItAll's integrated toolsets eliminate the need for multiple software packages and increase overall lab efficiency and staff proficiency. Combined with Wiley’s comprehensive, high-quality spectral reference libraries, it provides an unparalleled solution for fast, reliable spectral analysis. Learn more.<h4>About <a target="_blank" rel="noopener noreferrer" href="https://www.wiley.com/en-ie/">Wiley</a></h4>Wiley (NYSE: WLY) is a global leader in authoritative content and research intelligence for the advancement of scientific discovery, innovation, and learning. With more than 200 years at the center of the scholarly ecosystem, Wiley combines trusted publishing heritage with AI-powered platforms to transform how knowledge is discovered, accessed, and applied. From individual researchers and students to Fortune 500 R&amp;D teams, Wiley enables the transformation of scientific breakthroughs into real-world impact. From knowledge to impact—Wiley is redefining what's possible in science and learning. Visit us at Wiley.com and Investors.Wiley.com. Follow us on Facebook, X, LinkedIn and Instagram.

KnowItAll 2026.1 maintenance release now available - Upgrade for the latest features and fixes

<h3>Introduction</h3>Modern analytical laboratories often operate multiple chromatographic instruments simultaneously, which places high demands on software usability and workspace navigation. <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/999">Clarity Chromatography Software</a> developed by DataApex provides a structured, instrument-oriented user interface designed to simplify work even in complex multi-instrument environments. This article explains how to efficiently move between windows in Clarity.<h3>Concept of Instrument-Centric Workspace Organization</h3><h4>Instrument-Specific Color Schemes</h4>Each instrument in Clarity is visually distinguished by its own color scheme. This design choice allows users to:<ul><li>Instantly recognize which windows belong to the same instrument</li><li>Avoid confusion when multiple instruments are open simultaneously</li><li>Maintain orientation within complex analytical workflows</li></ul>Color coding is consistent across all windows associated with a given instrument.<h3>Types of Windows in Clarity</h3>Within a single instrument context, Clarity typically provides access to several interconnected windows, including:<ul><li>Instrument Window – the central control and overview window</li><li>Chromatogram Window – displays acquired chromatographic data</li><li>Sequence Window – manages sample sequences and runs</li></ul>Efficient navigation between these windows is essential for smooth operation.<h3>Navigation Methods Between Windows</h3>Clarity offers multiple complementary ways to switch between related windows. Users can choose the method that best fits their workflow preferences.<h4>1. Instrument Window Tiles</h4>Within the Instrument Window, large tiles represent related windows.Example workflow:<ul><li>Select the large chromatogram tile to switch directly from the Instrument Window to the Chromatogram Window</li></ul>This tile-based approach is intuitive and visually guided.<h4>2. Toolbar Icons</h4>Each instrument window contains a toolbar with window icons that provide direct access to other related windows.Key characteristics:<ul><li>The same set of window icons is available across all instrument-related windows</li><li>Enables quick transitions, for example:<ul><li>From Chromatogram Window → Sequence Window</li></ul></li><li>Ideal for experienced users who prefer icon-based navigation</li></ul><h4>3. Window Menu</h4>For users who prefer menu-driven control, Clarity includes a Window menu.Typical use case:<ul><li>Select Main from the Window menu to return to the Clarity main window</li></ul>This method is particularly useful when:<ul><li>Navigating back to the global workspace</li><li>Managing multiple instruments at once</li></ul><h3>Practical Navigation Workflow Example</h3>A typical navigation sequence demonstrated in the video follows this logic:<ol><li>Start in the Instrument Window</li><li>Click the Chromatogram tile to open chromatographic data</li><li>Use the toolbar icon to switch to the Sequence Window</li><li>Open the Window menu and select Main to return to the Clarity main interface</li></ol>This combination of tiles, icons, and menus ensures flexible and efficient workspace control.<h3>Advantages of Clarity’s Navigation Design</h3>The navigation system in Clarity provides several practical benefits:<ul><li>Consistency – identical navigation tools across instrument windows</li><li>Efficiency – rapid switching without losing context</li><li>Scalability – effective even with many instruments open</li><li>User preference flexibility – toolbar or menu-based workflows</li></ul>These features are especially valuable in high-throughput laboratories and 24/7 operational environments.<h3>Conclusion</h3>Clarity Chromatography Software uses a well-structured, instrument-oriented window system that simplifies navigation even in complex multi-instrument setups. By combining color-coded instruments, tile-based access, universal toolbar icons, and traditional menus, Clarity allows users to move quickly and confidently between windows.Mastering these navigation techniques significantly improves workflow efficiency, reduces errors, and enhances overall user experience—making Clarity a robust platform for modern chromatographic laboratories.For additional tutorials and practical demonstrations, users are encouraged to explore further educational resources provided by DataApex.

How to switch between the Clarity Windows

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ECOM ECS06 Gradient Analytical System

Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump, a gradient box, and an autosampler with/without cooling and heating, 40 MPa (5801 psi).

<div style="display:block;" aria-labelledby="tab_item-0">Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump, a gradient box, and an autosampler with/without cooling and heating, 40 MPa (5801 psi).The detector with eight channels in the range of 200-800 nm and a very low noise level also offers high-speed scanning up to 100 Hz. The units are communicated through RS232 or Ethernet (LAN). An additional PC A/D converter card is not required for this system. The system contains an autosampler and Clarity software. All units are supported in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a> from installation.A wireless mouse and keyboard set (P/N: EPC-0028), a PC (P/N: EPC-0026), and a computer monitor (P/N: EPC-0027) can be purchased with the system.<h3>Main parts</h3><h5>ECDA2800 UV-VIS PDA DETECTOR</h5>Analytical Diode Array Detector measures at eight wavelengths simultaneously or performs a full scan with a speed of up to 100 Hz. 3D support in Clarity SW. Noise level ±5 x 10-6 AU. Four analog outputs. Two special mathematical channels are available. AD 05 analytical cell is included.<h5>ECO2080L COLUMN OVEN WITH HEATING AND COOLING</h5>This column oven allows precise temperature stabilization in the range of 0-80°C. A big compartment room can accommodate up to 3 columns with lengths up to 30 cm. Peltier technology offers the possibility of heating and cooling independently from room temperature.<h5>ECP2010 ANALYTICAL HPLC PUMP</h5>This isocratic pump works with Gradient Box ECB2004B as a quaternary gradient pump. The flow rate range is 0.001-10.00 ml/min and the pressure limit is 40 MPa. Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.<h5>ECB2004B GRADIENT BOX WITH DEGASSER</h5>The box is suited for handling liquids in ECOM gradient systems. It accommodates a container for solvent bottles, a built-in high-efficiency vacuum degasser, and a four-way gradient valve. Up to four solvent lines may be degassed simultaneously by one unit. A four-way gradient valve is controlled from the pump ECP2010.<h5>AUTOSAMPLER AS96</h5>A reliable and precise autosampler for a wide range of applications in HPLC and LC-MS in variants with or without a cooling and heating module. State-of-the-art injection technology with fast injection and wash cycles. For 2x48 vials, max. pressure 40 MPa (5801 psi)<h5>CLARITY CHROMATOGRAPHY SOFTWARE</h5>This system is fully controlled by Clarity Chromatography software, which is also a part of delivery. Clarity software complies with CFR21GLP requirements.<h3>SYSTEM CONTAINS&nbsp;</h3>Pcs / Description (P/N)<ul><li>1x / ECDA2800 Analytical UV-VIS PDA Detector (ANW0000X)</li><li>1x /&nbsp;ECB2004B Gradient box with degasser (ABA0000X)</li><li>1x / ECP2010 Analytical HPLC pump (ACA0000X)</li><li>1x / ECO2080L Column oven with heating and cooling (AOA00004)</li><li>1x / Autosampler AS96 for 2x48 vials, 40 MPa, with or without heating and cooling module (ASA97010/ASA97000)</li><li>1x / Clarity – Station for 1 instrument (AP000C50)</li><li>1x / Clarity module for HPLC control (AP000A24)</li><li>1x / Clarity SW module for AS controlvAP000A26)</li><li>1x / Module for PDA data Clarity SW (AP000A29)</li><li>1x / Accessories for analyt. system series 2000 (ASA00180)</li><li>1x / Accessories for autosampler (ASA96180)</li></ul>Note: Column and computer not included. PC system has to be equipped for handling 3 serial RS232 or 1 Ethernet port at least. Please, check also <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a> requirements..<h3>Configuration options</h3><ul><li>ASA0600X - Gradient analytical system - incl. autosampler without cooling and heating, up to 40 MPa (5801 psi)</li><li>ASA06C0X - Gradient analytical system – incl. autosampler with cooling and heating, up to 40 MPa (5801 psi)</li></ul></div>

ECOM ECS07 Gradient Analytical System

Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump (60 MPa, 8702 psi), a gradient box, and an autosampler.

<div style="display:block;" aria-labelledby="tab_item-0">Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump, a gradient box, and an autosampler with/without cooling and heating, 60 MPa (8702 psi).The detector with eight channels in the range of 200-800 nm and a very low noise level also offers high-speed scanning up to 100 Hz. The units are communicated through RS232 or Ethernet (LAN). An additional PC A/D converter card is not required for this system. The system contains an autosampler and Clarity software. All units are supported in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a> from installation.A wireless mouse and keyboard set (P/N: EPC-0028), a PC (P/N: EPC-0026), and a computer monitor (P/N: EPC-0027) can be purchased with the system.<h3>Main parts</h3><h5>ECDA2800 UV-VIS PDA DETECTOR</h5>Analytical Diode Array Detector measures at eight wavelengths simultaneously or performs a full scan with a speed of up to 100 Hz. 3D support in Clarity SW. Noise level ±5 x 10-6 AU. Four analog outputs. Two special mathematical channels are available. AD 05 analytical cell is included.<h5>ECO2080L COLUMN OVEN WITH HEATING AND COOLING</h5>This column oven allows precise temperature stabilization in the range of 0-80°C. A big compartment room can accommodate up to 3 columns with lengths up to 30 cm. Peltier technology offers the possibility of heating and cooling independently from room temperature.&nbsp;<h5>ECP2010H ANALYTICAL HPLC PUMP</h5>This isocratic pump works with Gradient Box ECB2004B as a quaternary gradient pump. The flow rate range is 0.001-10.00 ml/min and the pressure limit is 60 MPa. Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.<h5>ECB2004B GRADIENT BOX WITH DEGASSER</h5>The box is suited for handling liquids in ECOM gradient systems. It accommodates a container for solvent bottles, a built-in high-efficiency vacuum degasser, and a four-way gradient valve. Up to four solvent lines may be degassed simultaneously by one unit. A four-way gradient valve is controlled from the pump ECP2010.<h5>AUTOSAMPLER AS96</h5>A reliable and precise autosampler for a wide range of applications in HPLC and LC-MS in variants with or without a cooling and heating module. State-of-the-art injection technology with fast injection and wash cycles. Max. pressure 60 MPa.<h5>CLARITY CHROMATOGRAPHY SOFTWARE</h5>This system is fully controlled by Clarity Chromatography software, which is also a part of delivery. Clarity software complies with CFR21GLP requirements.<h3>SYSTEM CONTAINS&nbsp;</h3>Pcs / Description (P/N)<ul><li>1x / ECDA2800 Analytical UV-VIS PDA Detector (ANW0000X)</li><li>1x /&nbsp;ECB2004B Gradient box with degasser (ABA0000X)</li><li>1x / ECP2010H Analytical HPLC pump (ACF0000X)</li><li>1x / ECO2080L Column oven with heating and cooling (AOA00004)</li><li>1x / Autosampler AS96 for 2x48 vials, 60 MPa, with or without heating and cooling module (ASA96000/ASA96010)</li><li>1x / Clarity – Station for 1 instrument (AP000C50)</li><li>1x / Clarity module for HPLC control (AP000A24)</li><li>1x / Clarity SW module for AS controlvAP000A26)</li><li>1x / Module for PDA data Clarity SW (AP000A29)</li><li>1x / Accessories for analyt. system series 2000 (ASA00180)</li><li>1x / Accessories for autosampler (ASA96180)</li></ul>Note: Column and computer not included. PC system has to be equipped for handling 3 serial RS232 or 1 Ethernet port at least. Please, check also <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a> requirements..<h3>Configuration options</h3><ul><li>ASA0700X - Gradient analytical system - incl. autosampler without cooling and heating, up to 60 MPa.</li><li>ASA07C0X - Gradient analytical system – incl. autosampler with cooling and heating, up to 60 MPa.</li></ul></div>

ECOM LED20M FIX Dual UV Detector with display

The LED20M FIX Dual UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.

<div style="display:block;" aria-labelledby="tab_item-0">The LED20M FIX Dual UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.<h3>Why choose LED20M FIX detectors for your application</h3><ul><li>The LED detectors allow easy and convenient detection in the UV-VIS range. They are manufactured in single or dual channel models with fixed wavelength setup.</li><li>The detectors are widely used in chromatography techniques using gravity glass columns for purification and isolation of organic small molecules and other compounds of interest. Long-lasting and reliable light source is provided by LED.</li><li>The optical flow cell is easily accessible and replaceable, this feature allows flexible choice of detection sensitivity if required. Low cell heating due to low power dissipation of LEDs makes this detector ideal for applications where analytes may be susceptible to degradation due to elevated temperatures. Absorbance information is displayed in detail on the integrated OLED display in numerical and graphical formats.</li><li>The units have convenient USB-C connectivity and power. When the detector is connected to a PC, the recorded data can be stored and processed using the ECOMAC software. ECOMAC basic software license is available with the price of the product without any additional fees. All these features and capabilities allow LED20M FIX to be an affordable, cost-effective, reliable and robust choice of an UV-VIS detector.</li></ul><h4>Wavelengths</h4><ul><li>The dual UV detector version measures at two fixed wavelengths, which are adjusted in production and given by the built-in LEDs.</li><li>By default, diodes with wavelengths of 255 and 280 nm are used. Optionally, the customer can select a wavelength in the range of 255–⁠⁠⁠⁠⁠⁠600 nm in steps of 5 nm.</li></ul><h4>Control</h4><ul><li>The detector can work without any additional SW support, but it is also possible to interconnect it via USB-C cable to a PC with ECOMAC SW, to get a data set for later use or to get reports for unit diagnostics.</li><li>The unit has an innovative PCB board that powers the LEDs, controls the unit, and has many new features, including higher sampling speed.</li></ul><h4>Key features and benefits</h4><ul><li>OLED display</li><li>Numerical and graphical view mode</li><li>Long lifetime of the LED light source</li><li>Low cell heating due to low power losses of the LEDs</li><li>USB C powering and communication port</li><li>ECOMAC Basic software for free</li><li>Autozero function</li><li>Easy flow cell replacement</li><li>Small dimensions</li><li>ECOMAC SW - &nbsp;sophisticated software for data acquisition and for diagnostics</li></ul></div>

ECOM LED20M FIX Single UV Detector with display

The LED20M FIX Single UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.

<div style="display:block;" aria-labelledby="tab_item-0">The LED20M FIX Single UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.<h3>Why choose LED20M FIX detectors for your application</h3><ul><li>The LED detectors allow easy and convenient detection in the UV-VIS range. They are manufactured in single or dual channel models with fixed wavelength setup.</li><li>The detectors are widely used in chromatography techniques using gravity glass columns for purification and isolation of organic small molecules and other compounds of interest. Long-lasting and reliable light source is provided by LED.</li><li>The optical flow cell is easily accessible and replaceable, this feature allows flexible choice of detection sensitivity if required. Low cell heating due to low power dissipation of LEDs makes this detector ideal for applications where analytes may be susceptible to degradation due to elevated temperatures. Absorbance information is displayed in detail on the integrated OLED display in numerical and graphical formats.</li><li>The units have convenient USB-C connectivity and power. When the detector is connected to a PC, the recorded data can be stored and processed using the ECOMAC software. ECOMAC basic software license is available with the price of the product without any additional fees. All these features and capabilities allow LED20M FIX to be an affordable, cost-effective, reliable and robust choice of an UV-VIS detector.</li></ul><h3>Wavelengths</h3><ul><li>The single UV detector version measures at 1 fixed wavelength, which is adjusted in production and given by the built-in LEDs.</li><li>By default, diodes with wavelengths of 255 or 280 nm are used. Optionally, customers can select a wavelength within the range of 255–⁠⁠⁠⁠⁠⁠600 nm in steps of 5 nm.</li></ul><h3>Control</h3><ul><li>The detector can work without any additional SW support, but it is also possible to interconnect it via USB-C cable to a PC with ECOMAC SW, to get a data set for later use or to get reports for unit diagnostics.</li><li>The unit has an innovative PCB board that powers the LEDs, controls the unit, and has many new features, including higher sampling speed.</li></ul><h3>Key features and benefits</h3><ul><li>Practical OLED display</li><li>Numerical and graphical view mode</li><li>Long lifetime of the LED light source</li><li>Low cell heating due to low power losses of the LEDs</li><li>USB -C powering and communication port</li><li>Autozero function</li><li>Easy flow cell replacement</li><li>Small dimensions</li><li>ECOMAC SW - &nbsp;sophisticated software for data acquisition and for diagnostics</li></ul>Note: A preparative cell PFC 200 Basic is supplied with the unit. Other flow cells are available at request.</div>

ECOM LED20G Dual UV Detector

LED20G Dual UV detector for preparative and flash chromatography. ECOM's unique patented technology radically improves the detectors´ features. LEDs are used as the source of light.

<div style="display:block;" aria-labelledby="tab_item-0">LED20G Dual UV LED detector for preparative and flash liquid chromatography. LEDs (Light Emitting Diodes) are used as the light source. A unique and patented ECOM technology radically improves the detector characteristics.&nbsp;The LEDs themselves typically have a peak wavelength with a tolerance of +/- 5 nm and a spectral half-width of 10-20 nm, which is unacceptable for some applications, as e.g. for FPLC. This ECOM solution, however, allows the spectral width to be narrowed and, thanks to our patented technology, the exact wavelength to be selected from the LED spectrum.This version of the dual UV detector measures at two fixed wavelengths given by the installed LEDs.The default wavelengths are 254 and 280 nm. Optionally, the customer can select a wavelength in the range 254-600 nm in 1 nm steps.The unit is controlled by an innovative printed circuit board that powers the LEDs, controls the unit, and has many new features including higher sampling speed.<h3>Key features and benefits</h3><ul><li>Sharp, high, and symmetrical peaks thanks to the ECOM patented technology</li><li>Excellent linearity</li><li>Excellent price-performance ratio</li><li>Compact device dimensions</li><li>Low cell heating due to LED low power supply (0.7 W) allows use for peptide analysis or FPLC</li><li>Easy flow cell replacement</li><li>Higher sampling speed</li><li>Sophisticated diagnostic software</li><li>Diodes with wavelengths of 254 and 280 nm and a PFC cell with an optical path length of 2 mm are used as standard.</li><li>Other cells and wavelengths on request.</li></ul><h3>Parameters</h3><ul><li>Number of channels (Signals): Two fix wavelength</li><li>Typical spectral half-width&nbsp;: 4</li><li>Light source: LEDs</li><li>Noise level at test cell (254 nm, TC 0.75 s): ± 10x10-6 AU</li><li>Drift at test cell (254 nm after 1 h): 1x10-3 AU/hr</li><li>Communication: Remote digital I/O</li><li>Power supply: 12-24 V DC</li><li>Power input: 2 W</li><li>Materials in contact with mobile phase: FEP; fused silica, stainless steel (cell)</li><li>Communication interface: USB-C</li><li>Optical cables: no</li><li>Dimensions W x H x D: 193 x 70 x 40 mm (7.60 x 2.76 x 1.57 in)</li><li>Weight: 0.66 kg (1.46 Ibs)</li></ul></div>

ECOM L3320 HPLC Autosampler

An automatic autosampler with excellent accuracy and linearity of sample injection. Innovated needle design reduces sample contamination and µl pick-up mode achieves zero sample loss.

Autosampler L3320 is an automatic autosampler with excellent accuracy and linearity of sample injection. Innovated needle design significantly reduces sample residue contamination. µl pick-up mode achieves zero sample loss.The autosampler is designed as a&nbsp;standalone unit.The user-friendly unit features computer control and shows information about the actual status on the unit display. Automated self-checking simplifies the usage of the autosampler, there is no need for calibration or needle position setting.Rheodyne valve secures the reliable operation of the device.The unit is supported by the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity&nbsp;software</a>.<h4>Parameters</h4><ul><li>Communication: RS232,USB</li><li>Syringe volume: Standard 250μl; 100μl optional</li><li>Reproducibility: Full loop injection: RSD≤0.3%, Partial loopfill injection: RSD≤0.5%, μl pickup injection: RSD≤1%</li><li>Control:&nbsp;PC (Clarity SW)</li><li>Loop volumes: Standard 100μl; 10μl, 20μl and 50μl optional</li><li>Injection Volume: Full loop injection = loop volume, Partial loop injection = ½ of loop volume, μl pickup injection = (loop volume – 3 x needle volume)/2</li><li>Sample Capacity: 2 standard 48-vial tray (1.5ml vials)</li><li>Pressure limit: 6000 PSI</li><li>Cross Contamination: &lt; 0.02% after programmed needle wash</li><li>Weight: 17kg</li><li>Dimensions W x H x D: 453 x 359 x 244 mm (17.8 x 14.1 x 9.6”)</li><li>Linearity: 0.9999</li><li>Power supply: 100-240VAC, 50-60Hz, 110W&nbsp;</li></ul>

Products focused on HPLC from ECOM - page 1

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