Understanding the fundamentals of the on-off retention mechanism of oligonucleotides and their application to high throughput analysis

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 13th January 2025? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications by <strong>Thermo Fisher Scientific,</strong> <strong>Shimadzu</strong>,<strong> </strong>a presentation by <strong>Agilent Technologies</strong>, a manual by <strong>Metrohm,</strong> and a poster from <strong>ASMS </strong>by <strong>Waters Corporation</strong>!</p><h4><strong>1. Thermo Fisher Scientific: High-resolution DIA proteomics workflow for single-cell samples on the Orbitrap Astral mass spectrometer</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/28638"><strong>Full PDF for download</strong></a></li></ul><p>Assess proteome coverage and sample throughput performance for single-cell samples using a library-free and library-based data-independent acquisition (DIA) method on the Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer.</p><h5><strong>Introduction</strong></h5><p>In recent years, there has been a shift to analyzing smaller sample quantities, driven by the interest in profiling the proteome of individual cells. This increased focus on LC-MS analysis of limited sample amounts, e.g., single cells, requires the highest possible sensitivity. At the same time, throughput and chromatographic performance must be preserved to generate sufficiently high-quality data from large data sets to draw meaningful conclusions. This trend toward analyzing smaller sample quantities reflects the need to understand the heterogeneous nature of biology through the dissection of complex systems into their individual parts or, specifically, individual cells. Traditional proteomics approaches read out the average of a sample while single-cell proteomics provides insights into the exact nature of each of the components in such samples.</p><p>The Orbitrap Astral mass spectrometer delivers a new standard for single-cell samples with deeper proteome coverage and faster throughput. It enables these capabilities through the synchronized acquisition of high-resolution and high-dynamic range full scan spectra with the Thermo Scientific™ Orbitrap™ mass analyzer and high speed and sensitivity MS/MS spectra with the Thermo Scientific™ Astral™ mass analyzer. The Thermo Scientific™ Vanquish™ Neo UHPLC system delivers maximum performance in terms of precise, low flow rates and gradient formation, as well as low injection volumes—an important benefit for single-cell proteomics and limited sample amounts.</p><p>The Thermo Scientific™ μPAC™ Neo HPLC columns with novel micro-pillar array deliver highly reproducible chromatographic separation and increased sensitivity with maximum resolution.<br>The Thermo Scientific™ FAIMS Pro Duo interface enhances sensitivity by removing background signals.</p><p>In this technical note, we present a single-cell proteomics workflow (Figure 1). To first evaluate sensitivity and quantitative precision and accuracy and linearity of the workflow, a dilution series from 5 ng to 50 pg of the Thermo Scientific™ Pierce™ HeLa protein digest standard was analyzed.</p><h4><strong>2. Shimadzu: Automatic Optimization of Gradient Conditions by AI Algorithm - Consecutive Optimization at Different Column Oven Temperatures</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/28642"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>The AI algorithm of LabSolutions MD can automatically optimize gradient conditions to greatly reduce labor of LC method development.</li><li>Gradient conditions are automatically optimized at different column oven temperatures consecutively.</li><li>Automatic optimization of gradient conditions can be applied not only to new method development, but also to existing method to efficiently improve resolution.</li></ul><h5><strong>Introduction</strong></h5><p>In the typical LC method development, the process begins with “preparation” which includes mobile phase preparation, column installation, and creation of analysis schedules, then the analysis is started. After that, the acquired data is analyzed and “preparation” for the subsequent analysis is carried out, followed by starting the next analysis again. The method development progresses by repeating these processes, but in addition to the significant time required to repeatedly create analysis schedules, expertise in chromatography is necessary to explore optimal conditions based on data analysis. In other words, typical method development requires “human intervention”. Therefore, eliminating human involvement and automating such method development processes would be desirable to improve labor efficiency. This article introduces an approach for automatically optimizing gradient conditions at different column oven temperatures consecutively, identifying the combination of temperature and gradient conditions that meet the resolution criteria.</p><h5><strong>Conclusion</strong></h5><p>Automatic optimization of gradient conditions using AI algorithm of LabSolutions MD was applied to a model sample (mixture of seven compounds of small molecule) at different column oven temperatures. As a result, the temperature and gradient conditions that met the resolution criteria were successfully explored. In method development, human intervention, such as analysis batch creation and data analysis, is required to optimize gradient conditions. LabSolutions MD can provide significant labor savings in this area. For more information on LabSolutions MD, please refer to the Technical Report <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/c190_e284_1ca86b48ea/c190-e284.pdf">“Efficient Method Development Based on Analytical Quality by Design with LabSolutions MD Software (C190-E284)”</a>.</p><h4><strong>3. Agilent Technologies: Optimize Your Polymer Analysis by Selecting the Ideal GPC/SEC Column</strong></h4><ul><li>Presentation</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/28578"><strong>Full PDF for download</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4481"><strong>Recorded webinar</strong></a></li></ul><p>&nbsp;</p><ul><li><strong>GPC/SEC Separation Mechanism</strong></li><li><strong>Conventional GPC/SEC Workflow</strong></li><li><strong>Considerations for GPC/SEC Column Selection (Key questions to ask)</strong><ul><li>What polymer are you analysing?</li><li>Which solvent (or solvents) is your polymer soluble in?</li><li>What is the expected molecular weight range of your polymer?</li><li>What is the requirement for your analysis or what would you like to improve about your existing GPC/SEC separation?<ul><li>Resolution is important</li><li>Reproducibility of sample chromatography and results</li><li>Speed of analysis or sample throughput is something to improve on</li></ul></li></ul></li><li><strong>Further considerations</strong><ul><li>Know the properties of the sample</li><li>Be familiar with the properties of the columns being considered</li><li>It is important to balance polarities for the sample, solvent, and column packing</li></ul></li><li><strong>Common Column Chemistries for GPC/SEC</strong></li><li><strong>Agilent Magic Triangles</strong></li><li><strong>Column Selection: Porosity</strong></li><li><strong>Individual Pore Versus MultiPorous Particle</strong></li><li><strong>Column Selection: Particle size</strong></li><li><strong>Columns for Low Polarity Organic Eluents</strong></li><li><strong>Columns for Medium Polarity Organic Eluents</strong></li><li><strong>Columns for High Temperature GPC/SEC</strong></li><li><strong>Columns for Fluorinated Organic Eluents</strong></li><li><strong>Columns for Aqueous Eluents: Neutral or weak Anions</strong></li><li><strong>Columns for Aqueous Eluents: Polycations and Strong Polyanions</strong></li><li><strong>Column Selection: Additional Considerations</strong></li><li><strong>Columns in Series</strong></li><li><strong>Wrongly Coupled Columns</strong></li><li><strong>Combination of Individual Pore Size Columns</strong></li><li><strong>Column Selection and Importance of Solvent Choice</strong></li><li><strong>Solvent Selection</strong></li><li><strong>Successful Solvent Choice</strong></li><li><strong>Eluent Modification in Organic GPC</strong></li><li><strong>Common Questions Around GPC/SEC Standards</strong></li><li><strong>Agilent Polymer Calibration Standard Offerings</strong></li><li><strong>Well Characterized Polymer Standards</strong></li><li><strong>Easivial Certificate of Analysis</strong></li><li><strong>Common Questions Around SEC Standards</strong></li><li><strong>Installation and Care of Your Column</strong></li><li><strong>Before Your Analysis</strong></li><li><strong>Column Selection – Additional considerations</strong></li><li><strong>System Tubing</strong></li><li><strong>Your GPC/SEC Toolbox</strong></li></ul><h4><strong>4. Metrohm: Installation Instruction for ProfIC Vario 7 Cation</strong></h4><ul><li>Manual</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/28584"><strong>Full PDF for download</strong></a></li></ul><p>The ProfIC Vario 7 Cation is a Professional IC Vario system with Inline Dilution and Inline Dialysis for the fully automatic determination of cations in high concentrations in matrices containing emulsions. This system enables users to dilute the sample and eliminate droplets of fat or organic solvents or particles prior to the analysis.</p><h4><strong>5. Waters Corporation: IMPROVED TISSUE HOMOGENIZATION AND SPE-BASED SAMPLE PREPARATIONS FOR THE QUANTITATIVE LC-MS ANALYSIS OF OLIGONUCLEOTIDE THERAPEUTICS</strong></h4><ul><li>Poster/ASMS</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/27580"><strong>Full PDF for download</strong></a></li></ul><p>Efficient extraction of drug analytes is a critical aspect of drug metabolism and pharmacokinetics (DMPK) studies. This has long applied to small molecules and still applies to the bioanalysis of oligonucleotides. Oligonucleotide drugs and their metabolites must be quantified within both biofluids and tissue samples. The newest of oligonucleotide drugs are both extensively modified and conjugated. These modified residues and conjugate moieties can complicate extraction recovery and reproducibility. In this work, we report several key insights on how to achieve improved extractions. Protocols for solvent-assisted proteinase K sample pre-treatments are investigated using a weak anion exchange (WAX) microplate-based solid phase extraction (SPE) device. Direct injection LC-MS quantitation is demonstrated for all three antisense oligonucleotides (ASOs).</p><h5><strong>CONCLUSIONS</strong></h5><ul><li>Selective extraction and elution of ASO oligonucleotides is feasible from multiple tissue types.</li><li>Extracted ASOs are amenable for either full scan mode analysis on benchtop small footprint TOF MS or MRM analysis by Triple Quad MS</li><li>Reproducible and repeatable data were collected even without the application of an internal standard</li><li>One novel aspect of the SPE eluate is that no further sample preparation is required for subsequent LC-MS analysis saving time and avoiding sample losses during drying and reconstitution</li><li>Eluted nucleic acid components can be readily analyzed by regular ion-pairing reversed phase chromatography and UV/MS-based detection and quantitation</li><li>This robust tissue extraction protocols for improved LC-MS based bioanalysis of ASO drugs was repeatable and highly reproducible across multiple batches of SPE sorbent, providing confidence in the suitability of the optimized protocols</li></ul>

News from LabRulezLCMS Library - Week 3, 2025

<p><strong>In the week of January 20, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over&nbsp;</p><p><strong>4 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Characterization and sequence mapping of large RNA and mRNA therapeutics using HRAM</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4580">Registration</a></div></li></ul></div><p>The presentation showcases the innovative partial T1 digest strategy developed at the University of Sheffield for sequencing mRNA therapeutics.</p></div><h5><strong>2.&nbsp;SelectScience/Agilent Technologies: From Start to Finish: How Agilent Can Support Better Decisions in IVT mRNA-Based Biopharmaceutical Analysis</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4674">Registration</a></div></li></ul></div><p>We will discuss how automated electrophoresis and LC/MS systems solutions from Agilent provide reliable insights for IVT mRNA workflows at many steps.</p></div><h5><strong>3.&nbsp;Phenomenex: LC Troubleshooting Quick Guide: Spot Problems Fast &amp; Fix Them Like a Pro!</strong></h5><div><div><ul><li><div>Th, 23.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4676">Registration</a></div></li></ul></div><p>In this webinar - General troubleshooting approach, HPLC troubleshooting steps and diagnosing tips and preventive maintenance and column care.</p></div><h5><strong>4.&nbsp;Thermo Fisher Scientific: State-of-the-art E&amp;L is not rocket science</strong></h5><div><div><ul><li><div>Th, 23.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4581">Registration</a></div></li></ul></div><p>Ulli Honester, a team leader from Solvias, presented on advancements in mass spectrometry for identifying extractables and leachables (E&amp;L).</p></div><h5><strong>5.&nbsp;Waters Corporation: Empower Tips: Ask Me Anything About Navigating Data Governance - Risk and Vigilant Monitoring</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4550">Registration</a></div></li></ul></div><p>We will explore critical aspects of data governance and critical thinking, insights and strategies to help navigate the current environment, evolving your approach and modernizing your way of working.</p></div><h5><strong>6.&nbsp;Evosep Biosystems: A beginner’s guide to Evosep 2025</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4548">Registration</a></div></li></ul></div><p>This webinar is your opportunity to gain a comprehensive understanding of the Evosep One’s key features and how it can seamlessly integrate into your current processes.</p></div><h5><strong>7.&nbsp;Shimadzu Corporation: ASTM D8421 and D8535: Cost Effective Methods for Rapid PFAS Testing in Wastewater and Soil</strong></h5><div><div><ul><li><div>Th, 23.1.2025 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4465">Registration</a></div></li></ul></div><p>Discover simplified standardized methods for PFAS analysis based on co-solvation as sample preparation.</p></div>

Webinars LabRulezLCMS Week 4/2025

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Webinars

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Scientific article

Various

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

ECOM ECP201L Preparative HPLC Pump

Preparative HPLC pump with a flow rate range of up to 1000 ml/min.

<div style="display:block;" aria-labelledby="tab_item-0"><p>The <strong>ECP201L Preparative Pump</strong> is an innovative, powerful, and cost-effective unit for preparative liquid chromatography where high pressure is needed.&nbsp;The pump with 4 low-pressure valves delivers a flow rate of up to <strong>1000 ml/min</strong> at a very&nbsp;high output pressure of up to <strong>15 MPa (2176 psi).</strong></p><h3><strong>Main features and benefits</strong></h3><ul><li><strong>Durability&nbsp;</strong>– The pump is constructed for high pressure and flow rates. This makes it suitable for day-to-day long-term use in production</li><li><strong>Wide application possibilities</strong> – The unit is used for pharmaceutical separations and plant extract purification, such as cannabinoids, peptides, and oligonucleotides. Also, it has much wider use in other chemical production, such as dosing into chemical reactors, and in various laboratory uses. Another advantage is the usability of the same device for separation method development.</li><li><strong>Smart controls and diagnostics</strong>&nbsp;– It is easy to operate, especially with ECOMAC and Clarity software. This makes unit management powerful and convenient. The <strong>basic version</strong> of the <strong>ECOMAC software</strong> is available <strong>free of charge</strong>. It is also used f<strong>or service and diagnostics</strong>.</li><li><strong>Other useful features</strong><ul style="list-style-type:circle;"><li>A new learning algorithm for <strong>pulsation suppression</strong></li><li>A <strong>leakage sensor</strong></li><li>A <strong>seal wash kit for longer piston seal life</strong></li><li>Also available in configurations made of&nbsp;<strong>certified bio-inert materials</strong></li></ul></li></ul><h4><strong>Parameters</strong></h4><ul><li><strong>Flow rate: </strong>1– 1000 ml/min</li><li><strong>Maximum operating pressure: </strong>15 MPa up to 1000 ml/min</li><li><strong>Flow rate setting: </strong>1 ml/min steps</li><li><strong>Repeatability of flow rate adjusting:&nbsp;</strong>± 1 %</li><li><strong>Accuracy of flow rate setting:&nbsp;</strong>± 2 %</li><li><strong>Wetted materials:&nbsp;</strong>stainless steel, PEEK, TefzelTM, PE, ceramic, seals</li><li><strong>Communication:&nbsp;</strong>RS232,Ethernet (LAN),USB,Remote digital I/O</li><li><strong>Power supply:&nbsp;</strong>100-240V VAC</li><li><strong>Power input: </strong>500 VA</li><li><strong>Dimensions W x H x D:&nbsp;</strong>276 x 153 x 620 mm (10.87 x 6.14 x 24.40 in)</li><li><strong>Weight:&nbsp;</strong>26 kg (57.32 lb)</li><li><strong>Pressure Compensation Learning:&nbsp;</strong>yes&nbsp;</li></ul></div>

ECOM PREPBOX A3L8E Chromatography Separation System

PREPBOX is a compact alternative to modular preparative systems. It is assembled according to the customer's wishes.

<div style="display:block;" aria-labelledby="tab_item-0"><p><strong>Complete preparative modular system</strong> with one basic gradient pump and optionally secondary pump for repetitive sampling or with injection valve. Also switching valve for&nbsp;<abbr title="Countercurrent Chromatography"><strong>CCC</strong></abbr><strong> applications</strong>. PDA four channel detector up to 800&nbsp;nm.&nbsp;It is assembled according to the customer's wishes. It is used for high-capacity systems or with centrifugal chromatography systems like in pharmaceutical purification of <strong style="caret-color:auto;">cannabis extracts</strong><span style="caret-color:auto;"> (</span><abbr title="Cannabidiol"><span style="caret-color:auto;"><strong>CBD</strong></span></abbr><span style="caret-color:auto;">, </span><abbr title="Cannabinol"><span style="caret-color:auto;"><strong>CBN</strong></span></abbr><span style="caret-color:auto;">, </span><abbr title="Cannabigerol"><span style="caret-color:auto;"><strong>CBG</strong></span></abbr><span style="caret-color:auto;">, </span><abbr title="Cannabichromene"><span style="caret-color:auto;"><strong>CBC</strong></span></abbr><span style="caret-color:auto;">, </span><abbr title="Tetrahydrocannabinol"><span style="caret-color:auto;"><strong>THC</strong></span></abbr><span style="caret-color:auto;">...).</span></p><p><strong>ECOMAC SW</strong> installed at internal PC controls the system. Fractions are collected by means of&nbsp;<strong>10 position valve</strong>&nbsp;<br><br><strong>It is necessary to purchase an operating system for the PREPBOX</strong>&nbsp;- in most cases we recommend <strong>Windows OS</strong>&nbsp;(P/N:&nbsp;ABB90000) or <strong>Linux</strong>&nbsp;(on request).<br>The system also supports the Clarity software. It is also possible to purchase a wireless mouse and keyboard set&nbsp;(P/N:&nbsp;EPC-0028) and a computer monitor&nbsp;(P/N:&nbsp;EPC-0027).</p><h5><strong>TOY18DAD 800L Detector</strong></h5><p>It is UV-VIS diode array detector, which allow measuring absorbance of four wavelengths in range of 200-800&nbsp;nm simultaneously in one cell just as scanning of whole spectra.</p><h5><strong>ECP201L Preparative HPLC Pump</strong></h5><p>Preparative pump suited for preparative liquid chromatography with very high pressure <strong>up to 15&nbsp;MPa</strong>&nbsp;at flow rate of <strong>1000&nbsp;ml/min</strong>. Advantage of this pump is a very stable run even at low flow rates.</p><h5><strong>ECP2300 Preparative Gradient Pump</strong></h5><p><strong>Preparative HPLC pump</strong> suited for&nbsp;<strong>preparative</strong>&nbsp;applications in liquid chromatography with a pressure <strong>up to 15 MPa</strong> at flow rate of <strong>300&nbsp;ml/min</strong>.&nbsp;New sophisticated unit allows achieving a precise&nbsp;<strong>low-pressure gradient</strong>, i.e. gradual changes of solvent composition by mixing&nbsp;<strong>up to four liquids</strong>&nbsp;at the pump entry.</p><h3><strong>Parameters</strong></h3><ul><li><strong>Interface: </strong>5x USB, HDMI, 2X RS232, LAN, VGA</li><li><strong>Power supply: </strong>100 - 240 V AC</li><li><strong>Power input: </strong>490 W</li><li><strong>Dimensions W x H x D: </strong>405x490x525 mm (15.94x19.29x20.67 in)</li><li><strong>Weight: </strong>55 kg</li></ul></div>

<div style="display:block;" aria-labelledby="tab_item-0"><p><strong>Complete preparative modular system</strong> with one basic gradient pump and optionally secondary pump for repetitive sampling or with injection valve. Also switching valve for&nbsp;<abbr title="Countercurrent Chromatography"><strong>CCC</strong></abbr><strong> applications</strong>. PDA four channel detector up to 800&nbsp;nm.&nbsp;It is assembled according to the customer's wishes. It is used for high-capacity systems or with centrifugal chromatography systems like in pharmaceutical purification of <strong style="caret-color:auto;">cannabis extracts</strong><span style="caret-color:auto;"> (</span><abbr title="Cannabidiol"><span style="caret-color:auto;"><strong>CBD</strong></span></abbr><span style="caret-color:auto;">, </span><abbr title="Cannabinol"><span style="caret-color:auto;"><strong>CBN</strong></span></abbr><span style="caret-color:auto;">, </span><abbr title="Cannabigerol"><span style="caret-color:auto;"><strong>CBG</strong></span></abbr><span style="caret-color:auto;">, </span><abbr title="Cannabichromene"><span style="caret-color:auto;"><strong>CBC</strong></span></abbr><span style="caret-color:auto;">, </span><abbr title="Tetrahydrocannabinol"><span style="caret-color:auto;"><strong>THC</strong></span></abbr><span style="caret-color:auto;">...).</span></p><p><strong>ECOMAC SW</strong> installed at internal PC controls the system. Fractions are collected by means of&nbsp;<strong>10 position valve</strong>&nbsp;<br><br><strong>It is necessary to purchase an operating system for the PREPBOX</strong>&nbsp;- in most cases we recommend <strong>Windows OS</strong>&nbsp;(P/N:&nbsp;ABB90000) or <strong>Linux</strong>&nbsp;(on request). The system also supports the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity software</strong></a>. It is also possible to purchase a wireless mouse and keyboard set&nbsp;(P/N:&nbsp;EPC-0028) and a computer monitor&nbsp;(P/N:&nbsp;EPC-0027).</p><h5><strong>TOY18DAD 800L Detector</strong></h5><p>It is UV-VIS diode array detector, which allow measuring absorbance of four wavelengths in range of 200-800&nbsp;nm simultaneously in one cell just as scanning of whole spectra.</p><h5><strong>ECP2300 Preparative Gradient Pump</strong></h5><p><strong>Two peparative HPLC pump</strong> suited for&nbsp;<strong>preparative</strong>&nbsp;applications in liquid chromatography with a pressure <strong>up to 15 MPa</strong> at flow rate of <strong>300&nbsp;ml/min</strong>.&nbsp;New sophisticated unit allows achieving a precise&nbsp;<strong>low-pressure gradient</strong>, i.e. gradual changes of solvent composition by mixing&nbsp;<strong>up to four liquids</strong>&nbsp;at the pump entry.</p><h4><strong>Complete preparative modular system with:</strong></h4><ul><li>one basic gradient pump</li><li>for sampling secondary pump or injection valve</li><li>optionally switching valve for CCC</li><li>PDA detector up to 800 nm</li><li>10 positions valve for fractions collecting</li><li>PC board with ECOMAC SW</li></ul><h3><strong>Parameters</strong></h3><ul><li><strong>Interface: </strong>5x USB, HDMI, 2X RS232, LAN, VGA</li><li><strong>Power supply: </strong>100 - 240 V AC</li><li><strong>Power input: </strong>4900 W</li><li><strong>Dimensions W x H x D: </strong>405x490x525 mm (15.94x19.29x20.67 in)</li><li><strong>Weight: </strong>50 kg</li></ul></div>

ECOM ECS06 Gradient Analytical System

Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump, a gradient box, and an autosampler with/without cooling and heating, 40 MPa (5801 psi).

<div style="display:block;" aria-labelledby="tab_item-0"><p><strong>Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump, a gradient box, and an autosampler with/without cooling and heating, 40 MPa (5801 psi).</strong></p><p>The detector with eight channels in the range of 200-800 nm and a very low noise level also offers high-speed scanning <strong>up to 100 Hz</strong>. The units are communicated through <strong>RS232</strong> or Ethernet (LAN). An additional PC A/D converter card is not required for this system. The system contains an autosampler and Clarity software. All units are supported in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity software</strong></a> from installation.</p><p>A wireless mouse and keyboard set (P/N: EPC-0028), a PC (P/N: EPC-0026), and a computer monitor (P/N: EPC-0027) can be purchased with the system.</p><h3><strong>Main parts</strong></h3><h5><strong>ECDA2800 UV-VIS PDA DETECTOR</strong></h5><p>Analytical Diode Array Detector measures at eight wavelengths simultaneously or performs a full scan with a speed of up to 100 Hz. 3D support in Clarity SW. Noise level ±5 x 10-6 AU. Four analog outputs. Two special mathematical channels are available. AD 05 analytical cell is included.</p><h5><strong>ECO2080L COLUMN OVEN WITH HEATING AND COOLING</strong></h5><p>This column oven allows precise temperature stabilization in the range of 0-80°C. A big compartment room can accommodate up to 3 columns with lengths up to 30 cm. Peltier technology offers the possibility of heating and cooling independently from room temperature.</p><h5><strong>ECP2010 ANALYTICAL HPLC PUMP</strong></h5><p>This isocratic pump works with Gradient Box ECB2004B as a quaternary gradient pump. The flow rate range is 0.001-10.00 ml/min and the pressure limit is 40 MPa. Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.</p><h5><strong>ECB2004B GRADIENT BOX WITH DEGASSER</strong></h5><p>The box is suited for handling liquids in ECOM gradient systems. It accommodates a container for solvent bottles, a built-in high-efficiency vacuum degasser, and a four-way gradient valve. Up to four solvent lines may be degassed simultaneously by one unit. A four-way gradient valve is controlled from the pump ECP2010.</p><h5><strong>AUTOSAMPLER AS96</strong></h5><p>A reliable and precise autosampler for a wide range of applications in HPLC and LC-MS in variants with or without a cooling and heating module. State-of-the-art injection technology with fast injection and wash cycles. For 2x48 vials, max. pressure 40 MPa (5801 psi)</p><h5><strong>CLARITY CHROMATOGRAPHY SOFTWARE</strong></h5><p>This system is fully controlled by Clarity Chromatography software, which is also a part of delivery. Clarity software complies with CFR21GLP requirements.</p><h3>SYSTEM CONTAINS&nbsp;</h3><p><strong>Pcs / Description (P/N)</strong></p><ul><li>1x / ECDA2800 Analytical UV-VIS PDA Detector (ANW0000X)</li><li>1x /&nbsp;ECB2004B Gradient box with degasser (ABA0000X)</li><li>1x / ECP2010 Analytical HPLC pump (ACA0000X)</li><li>1x / ECO2080L Column oven with heating and cooling (AOA00004)</li><li>1x / Autosampler AS96 for 2x48 vials, 40 MPa, with or without heating and cooling module (ASA97010/ASA97000)</li><li>1x / Clarity – Station for 1 instrument (AP000C50)</li><li>1x / Clarity module for HPLC control (AP000A24)</li><li>1x / Clarity SW module for AS controlvAP000A26)</li><li>1x / Module for PDA data Clarity SW (AP000A29)</li><li>1x / Accessories for analyt. system series 2000 (ASA00180)</li><li>1x / Accessories for autosampler (ASA96180)</li></ul><p>Note: Column and computer not included. PC system has to be equipped for handling 3 serial RS232 or 1 Ethernet port at least. Please, check also <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity software</strong></a> requirements..</p><h3>Configuration options</h3><ul><li><strong>ASA0600X</strong> - Gradient analytical system - incl. autosampler <strong>without cooling and heating</strong>, up to 40 MPa (5801 psi)</li><li><strong>ASA06C0X</strong> - Gradient analytical system – incl. autosampler <strong>with cooling and heating</strong>, up to 40 MPa (5801 psi)</li></ul></div>

ECOM ECS07 Gradient Analytical System

Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump (60 MPa, 8702 psi), a gradient box, and an autosampler.

<div style="display:block;" aria-labelledby="tab_item-0"><p><strong>Highly effective HPLC system with a UV-VIS detector, a column oven, an analytical pump, a gradient box, and an autosampler with/without cooling and heating, 60 MPa (8702 psi).</strong></p><p>The detector with eight channels in the range of 200-800 nm and a very low noise level also offers high-speed scanning <strong>up to 100 Hz</strong>. The units are communicated through <strong>RS232</strong> or <strong>Ethernet (LAN)</strong>. An additional PC A/D converter card is not required for this system. The system contains an autosampler and Clarity software. All units are supported in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity software</strong></a> from installation.</p><p>A wireless mouse and keyboard set (P/N: EPC-0028), a PC (P/N: EPC-0026), and a computer monitor (P/N: EPC-0027) can be purchased with the system.</p><h3><strong>Main parts</strong></h3><h5><strong>ECDA2800 UV-VIS PDA DETECTOR</strong></h5><p>Analytical Diode Array Detector measures at eight wavelengths simultaneously or performs a full scan with a speed of up to 100 Hz. 3D support in Clarity SW. Noise level ±5 x 10-6 AU. Four analog outputs. Two special mathematical channels are available. AD 05 analytical cell is included.</p><h5><strong>ECO2080L COLUMN OVEN WITH HEATING AND COOLING</strong></h5><p>This column oven allows precise temperature stabilization in the range of 0-80°C. A big compartment room can accommodate up to 3 columns with lengths up to 30 cm. Peltier technology offers the possibility of heating and cooling independently from room temperature.&nbsp;</p><h5><strong>ECP2010H ANALYTICAL HPLC PUMP</strong></h5><p>This isocratic pump works with Gradient Box ECB2004B as a <strong>quaternary gradient pump</strong>. The flow rate range is <strong>0.001-10.00 ml/min</strong> and the pressure limit is <strong>60 MPa</strong>. Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.</p><h5><strong>ECB2004B GRADIENT BOX WITH DEGASSER</strong></h5><p>The box is suited for handling liquids in ECOM gradient systems. It accommodates a container for solvent bottles, a built-in high-efficiency vacuum degasser, and a four-way gradient valve. Up to four solvent lines may be degassed simultaneously by one unit. A four-way gradient valve is controlled from the pump ECP2010.</p><h5><strong>AUTOSAMPLER AS96</strong></h5><p>A reliable and precise autosampler for a wide range of applications in HPLC and LC-MS in <strong>variants with or without a cooling and heating module</strong>. State-of-the-art injection technology with fast injection and wash cycles. Max. pressure <strong>60 MPa.</strong></p><h5><strong>CLARITY CHROMATOGRAPHY SOFTWARE</strong></h5><p>This system is fully controlled by Clarity Chromatography software, which is also a part of delivery. Clarity software complies with CFR21GLP requirements.</p><h3>SYSTEM CONTAINS&nbsp;</h3><p><strong>Pcs / Description (P/N)</strong></p><ul><li>1x / ECDA2800 Analytical UV-VIS PDA Detector (ANW0000X)</li><li>1x /&nbsp;ECB2004B Gradient box with degasser (ABA0000X)</li><li>1x / ECP2010H Analytical HPLC pump (ACF0000X)</li><li>1x / ECO2080L Column oven with heating and cooling (AOA00004)</li><li>1x / Autosampler AS96 for 2x48 vials, 60 MPa, with or without heating and cooling module (ASA96000/ASA96010)</li><li>1x / Clarity – Station for 1 instrument (AP000C50)</li><li>1x / Clarity module for HPLC control (AP000A24)</li><li>1x / Clarity SW module for AS controlvAP000A26)</li><li>1x / Module for PDA data Clarity SW (AP000A29)</li><li>1x / Accessories for analyt. system series 2000 (ASA00180)</li><li>1x / Accessories for autosampler (ASA96180)</li></ul><p>Note: Column and computer not included. PC system has to be equipped for handling 3 serial RS232 or 1 Ethernet port at least. Please, check also <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity software</strong></a> requirements..</p><h3>Configuration options</h3><ul><li><strong>ASA0700X</strong> - Gradient analytical system - incl. autosampler <strong>without cooling and heating</strong>, up to 60 MPa.</li><li><strong>ASA07C0X </strong>- Gradient analytical system – incl. autosampler <strong>with cooling and heating</strong>, up to 60 MPa.</li></ul></div>

ECOM PREPSYSTEM - Chromatography Separation System

A complete flash/preparative/pilot scale/production modular system ready for customizable to the application needs.

<p>We would like to introduce an ECOM product news – <strong>a complete flash/preparative/pilot scale/production modular system with an excellent price/performance ratio.</strong></p><h3><strong>Applications</strong></h3><p>The system can be used e.g. in:</p><ul><li>high-capacity systems or with centrifugal liquid/liquid chromatography systems, like in pharmaceutical purification of plant extracts – e.g. cannabinoids (CBD, CBN, CBG, CBC, THC, etc.), peptides, and oligonucleotides, algae extracts, oleoresins, etc.,&nbsp;</li><li>Also in new molecule development or synthesis.</li></ul><h3><strong>Main parts</strong></h3><h4><strong>Pumps</strong></h4><ul><li>The device has 3 pumps, 2 main ones are intended for a "high" pressure gradient (parallel pump connection), and one of them has a <strong>6-solvent selection valve.</strong>&nbsp;</li><li>An optional third pump is used for repetitive sampling of high-volume samples. The unit also has a loop sample injection valve and a switching valve for <strong>CCC/CPC applications</strong> (ascending/descending mode).&nbsp;</li><li>The unit's universal <strong>PDA detector</strong> measures absorbance at <strong>four wavelengths at the same time up to 800 nm</strong>.</li></ul><h4><strong>Software</strong></h4><ul><li>The ECOMAC software or <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity software</strong></a> (FDA 21 CFR Part 11 compliant) is internally installed in the PC controlling the system.</li></ul><h4><strong>Customization</strong></h4><ul><li>The unit is completely customizable to the application needs to meet any scale criteria–from small flash sample separation testing through pilot scale prediction to high-capacity production.</li><li>It could work in ranges of <strong>10 ml/min.–6000 ml/min</strong> and it can be easily and automatically switched between these scales.</li></ul>

ECOM ECS23PC Quaternary Preparative Gradient System with Fraction Collector

A highly effective preparative system containing a UV-VIS DAD detector, a preparative pump, a gradient box with PC, and a fraction collector. The detector allows measuring at four wavelengths at the same time or an informative scan.

<div style="display:block;" aria-labelledby="tab_item-0"><p>A highly effective preparative system containing a UV-VIS DAD detector, a preparative pump, a gradient box with PC, and a fraction collector. The detector allows measuring at four wavelengths at the same time or an informative scan.</p><p>Communication is through <strong>RS232</strong> or <strong>Ethernet (LAN)</strong>. An additional PC A/D converter card is not required for this system. The system contains a manual injector and <strong>ECOMAC</strong> software which include drivers for all ECOM units.</p><p><strong>It is necessary to purchase an OS for the system</strong> - in most cases, we recommend OEM <strong>Windows</strong> OS (P/N: ABB90000) or <strong>Linux </strong>(on request).<br>It is also possible to purchase a wireless mouse and keyboard set (P/N: EPC-0028) and a computer monitor (P/N: EPC-0027).</p><h3><strong>Main parts</strong></h3><h4><strong>TOY18DAD H 600 DETECTOR</strong></h4><p>A UV-VIS diode array detector, which allows measuring the absorbance of <strong>four wavelengths in the range of 200 – 600 nm simultaneously in one cell</strong> just as visualization of whole spectra. It is possible to use the detector in f<strong>lash and preparative applications</strong>. Standardly is assembled preparative cell PLCC 15 but other cells are available.</p><h4><strong>ECP2300 PREPARATIVE PUMP</strong></h4><p>This isocratic pump works with Gradient Box ECB2007PC as a <strong>quaternary gradient pump</strong>. The flow rate range is <strong>0.1-300 ml/min</strong> and the pressure limit at <strong>15 MPa</strong>. Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.</p><h4><strong>ECB2005PC GRADIENT BOX WITH PC, WITHOUT DEGASSER</strong></h4><p>The box is suited for handling liquids in ECOM gradient systems. It accommodates a container for solvent bottles and <strong>a four-way </strong>gradient valve. A four-way gradient valve is controlled from the pump ECP2300. In this box is assembled an <strong>industrial PC board</strong>. This solution allows the installation of <strong>OS</strong> (Windows or Linux) and chromatography <strong>SW ECOMAC or Clarity</strong>.&nbsp;</p><h4><strong>ECF2096 FRACTION COLLECTOR</strong></h4><p>Fractions are collected according to a chosen method which can be created manually by keyboard, just as using <strong>ECOMAC</strong> software. Analog input allows collecting based on detector signal. Racks for 9, 21, and 40 ml tubes.</p><h4><strong>ECOMAC CHROMATOGRAPHY SOFTWARE</strong></h4><p>This system is fully controlled by ECOMAC software, which is also a part of delivery. It allows all units control, gradient creation, and fraction collecting.</p><h3><strong>SYSTEM CONTAINS&nbsp;</strong></h3><p><strong>Pcs / Description (P/N)</strong></p><ul><li>1x / TOY18DAD H 600 Four Channel Detector (ANW0000X)</li><li>1x / ECB2005PC Gradient Box with PC, without Degasser (ABBPC00X)</li><li>1x / ECP2300 Preparative Pump (ACE0000X)</li><li>1x / ECOMAC software (SA100000)</li><li>1x / ECF2096 Fraction collector (AFA0000X)</li><li>1x / Preparative injection valve 1/8”; 2ml loop (SY2032022)</li><li>1x / Accessory for prep. system series 2000 (ASP00180)</li></ul><p><strong>Note:</strong> The system can be equipped with detectors <strong>with different wavelengths ranges</strong> and pumps with <strong>different flow rates</strong> from ECOM production.</p></div>

ECOM ECP2100 Preparative Pump

 Preparative HPLC pump with a flow-rate range up to 100 ml/min. 

<div style="display:block;" aria-labelledby="tab_item-0"><p><strong>Innovated pumps ECP2100 work as isocratic pumps and together with a gradient box also as a gradient pump. </strong>These powerful and durable pumps are suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. They are designed with<strong> 2 pump heads connected in parallel with a piston diameter of 3/8”.</strong></p><p>Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. New material versions have been added-in, beyond to stainless steel A316L, also Hastelloy, titanium and PEEK for biocompatible applications.The pumps can operate in two modes, i.e. using <strong>constant pressure </strong>or<strong> using the newly added option of constant flow rate. </strong>The ECP2100 pump now has an innovated spring-loaded outlet check-valve.</p><h4><strong>Gradient function</strong></h4><p>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes <strong>ECB2007</strong>, <strong>ECB2007PC</strong> are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.</p><p>Customers can use the pistons‘ <strong>back-washing function</strong>, after working, for example, with a buffered mobile phase.</p><h4><strong>Seals</strong></h4><p>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.</p><h4><strong>Software support</strong></h4><p>The pumps are supported by <strong>ECOMAC and Clarity software</strong>.</p><h3><strong>Main features and benefits</strong></h3><ul><li>All models include a <strong>display and keyboard</strong> for manual control</li><li><strong>Precise low-pressure gradient mixing</strong> (when connected with a gradient module)</li><li>Very good <strong>durability and reliability</strong> with proper maintenance</li><li>Extended <strong>number of wetted material versions</strong> – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li><strong>Piston back-washing function</strong> for work with buffered or aggressive mobile phases</li><li><strong>New learning algorithm for pulsation suppression</strong></li><li>Integrated<strong> leakage sensor</strong></li><li><strong>Heating unit for pump head</strong>s available</li><li>Custom pump <strong>modification</strong>s of request</li></ul><p>This pump is equipped with the <strong>Pressure Compensation Learning algorithm</strong>. Thanks to this smart adaptive process, the <strong>pump regulates the rotation speed of the camshaft</strong> and measures its impact on pressure pulsation to <strong>minimize the output pulsation</strong> as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg" alt="ECOM: ECP2100 - Preparative Pump - Pressure Compensation Learning algorithm " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg 188w," sizes="100vw" width="700" height="580"></p><h3>Optional configurations/variants</h3><p><strong>Pump head heating unit,</strong> see the accessories shown below. If you would like this function for your pump, add the <strong>heating unit </strong>to&nbsp;your cart. (Also can be found in the Accessories product category).</p><p><strong>PN / Wetted parts of pumping block</strong></p><ul><li>ACCH000X / Hastelloy</li><li>ACCP000X /<span style="color:rgb(69,69,69);font-family:Hind, sans-serif;font-size:13px;"><span style="-webkit-text-stroke-width:0px;display:inline !important;float:none;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;"> </span></span>PEEK</li><li>ACC0000X / Stainless steel</li><li>ACCT000X / Titanium</li></ul><h4><strong>Parameters:</strong></h4><ul><li><strong>Flow rate: </strong>0.1 – 100 ml/min</li><li><strong>Maximum operating pressure: </strong>30 MPa (4351 psi)</li><li><strong>Flow rate setting: </strong>0.001 ml/min steps</li><li><strong>Repeatability of flow rate adjusting: </strong>± 0,5 %</li><li><strong>Accuracy of flow rate setting: </strong>± 2 %</li><li><strong>Communication:</strong> RS232, Ethernet (LAN), USB, Remote digital I/O</li><li><strong>Power supply: </strong>100 240V 50/60Hz 250VA</li><li><strong>Dimensions W x H x D: </strong>280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li><strong>Weight:</strong> 12 (26,5 lb)</li><li><strong>Output capillary outer diameter:</strong> 1/16”</li><li><strong>Upper pressure limit (MPa): </strong>1– 30 (4351PSI)</li><li><strong>Precision of pressure measurement: </strong>± 2%</li><li><strong>Supported Gradient Box: </strong>ECB2005, ECB2005PC</li><li><strong>Display, keypad: </strong>yes&nbsp;</li><li><strong>Pressure Compensation Learning: </strong>yes</li></ul></div>

LED20M FIX Dual UV Detector with display

The LED20M FIX Dual UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.

<div style="display:block;" aria-labelledby="tab_item-0"><p>The <strong>LED20M FIX Dual UV Detector</strong> with a display uses <strong>Light Emitting Diodes (LED) </strong>as the light source. It is used for flash chromatography.</p><h3><strong>Why choose LED20M FIX detectors for your application</strong></h3><ul><li>The LED detectors allow easy and convenient detection in the UV-VIS range. They are manufactured in single or dual channel models with fixed wavelength setup.</li><li>The detectors are widely used in chromatography techniques using gravity glass columns for purification and isolation of organic small molecules and other compounds of interest. Long-lasting and reliable light source is provided by LED.</li><li>The <strong>optical flow cell </strong>is <strong>easily accessible </strong>and <strong>replaceable</strong>, this feature allows flexible choice of detection sensitivity if required. Low cell heating due to <strong>low power dissipation of LEDs</strong> makes this detector ideal for applications where analytes may be susceptible to degradation due to elevated temperatures. Absorbance information is displayed in detail on the <strong>integrated OLED display </strong>in <strong>numerical and graphical formats</strong>.</li><li>The units have convenient USB-C connectivity and power. When the detector is connected to a PC, the recorded data can be stored and processed using the ECOMAC software. ECOMAC basic software license is available with the price of the product without any additional fees. All these features and capabilities allow LED20M FIX to be an affordable, cost-effective, reliable and robust choice of an UV-VIS detector.</li></ul><h4><strong>Wavelengths</strong></h4><ul><li>The dual UV detector version measures at two fixed wavelengths, which are adjusted in production and given by the built-in LEDs.</li><li>By default, diodes with wavelengths of 255 and 280 nm are used. Optionally, the customer can select a wavelength in the range of 255–⁠⁠⁠⁠⁠⁠600 nm in steps of 5 nm.</li></ul><h4><strong>Control</strong></h4><ul><li>The detector can work without any additional SW support, but it is also possible to interconnect it via USB-C cable to a PC with <strong>ECOMAC SW</strong>, to get a data set for later use or to get reports for unit diagnostics.</li><li>The unit has an innovative PCB board that powers the LEDs, controls the unit, and has many new features, including higher sampling speed.</li></ul><h4><strong>Key features and benefits</strong></h4><ul><li><strong>OLED display</strong></li><li><strong>Numerical and graphical view mode</strong></li><li><strong>Long lifetime</strong> of the <strong>LED</strong> light source</li><li><strong>Low cell heating </strong>due to <strong>low power losses of the LEDs</strong></li><li><strong>USB C</strong> powering and communication port</li><li><strong>ECOMAC Basic software for free</strong></li><li><strong>Autozero</strong> function</li><li><strong>Easy flow cell replacement</strong></li><li>Small dimensions</li><li><strong>ECOMAC SW </strong>- &nbsp;sophisticated software for data acquisition and for diagnostics</li></ul></div>

ECOM L3320 HPLC Autosampler

An automatic autosampler with excellent accuracy and linearity of sample injection. Innovated needle design reduces sample contamination and µl pick-up mode achieves zero sample loss.

<p>Autosampler L3320 is an automatic autosampler with excellent accuracy and linearity of sample injection. Innovated needle design significantly reduces sample residue contamination. µl pick-up mode achieves zero sample loss.</p><p>The autosampler is designed as a&nbsp;<strong>standalone unit</strong>.</p><p>The user-friendly unit features computer control and shows information about the actual status on the unit display. Automated self-checking simplifies the usage of the autosampler, there is no need for calibration or needle position setting.</p><p>Rheodyne valve secures the reliable operation of the device.</p><p>The unit is supported by the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity&nbsp;software</strong></a>.</p><h4><strong>Parameters</strong></h4><ul><li><strong>Communication</strong>: RS232,USB</li><li><strong>Syringe volume</strong>: Standard 250μl; 100μl optional</li><li><strong>Reproducibility</strong>: Full loop injection: RSD≤0.3%, Partial loopfill injection: RSD≤0.5%, μl pickup injection: RSD≤1%</li><li><strong>Control</strong>:&nbsp;PC (Clarity SW)</li><li><strong>Loop volumes</strong>: Standard 100μl; 10μl, 20μl and 50μl optional</li><li><strong>Injection Volume</strong>: Full loop injection = loop volume, Partial loop injection = ½ of loop volume, μl pickup injection = (loop volume – 3 x needle volume)/2</li><li><strong>Sample Capacity</strong>: 2 standard 48-vial tray (1.5ml vials)</li><li><strong>Pressure limit</strong>: 6000 PSI</li><li><strong>Cross Contamination</strong>: &lt; 0.02% after programmed needle wash</li><li><strong>Weight</strong>: 17kg</li><li><strong>Dimensions W x H x D</strong>: 453 x 359 x 244 mm (17.8 x 14.1 x 9.6”)</li><li><strong>Linearity</strong>: 0.9999</li><li><strong>Power supply</strong>: 100-240VAC, 50-60Hz, 110W&nbsp;</li></ul>

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