Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"><h4>The Role of Chromatography in Forensic Food Analysis</h4>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:<ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate adulteration.</li></ul>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.<h4>Isocratic vs. Gradient Elution</h4>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:<ul><li>Isocratic HPLC uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li>Gradient HPLC changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.<h4>Watrex Praha and the Contribution of Modern Systems to Food Analysis</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha, s.r.o.</a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline">Streamline™ product line</a> includes several HPLC system concepts suitable for food analysis:<ul><li>Isocratic HPLC system – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li>Binary gradient HPLC system – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures (e.g., phenolic profiles of wine, components of aromatic extracts).</li><li>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography) – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf">Product catalogue HERE</a></blockquote>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.<h4>Practical Examples of Adulteration Revealed by HPLC</h4><ul><li>Detection of olive oil dilution: HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li>Estimation of sugar addition in honey: Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li>Identification of aromatic adulterants in wine: Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4>Conclusion</h4>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.<blockquote>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true">LinkedIn</a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague">Facebook</a>) for more engaging topics.</blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.<h4>In this episode, you'll learn:</h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.<h4>Referenced Papers:</h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.<h3>Video Transcription</h3><h4>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</h4><h5>Introduction</h5>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.<h5>Research Focus: Malaria Drug Discovery and Kinase Targets</h5>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.<h5>Chromatography in Protein Expression and Purification Workflows</h5>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.<h5>Integrating Size Exclusion Chromatography into the Workflow</h5>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.<h5>The Role of Mass Spectrometry in Biomolecular Characterization</h5>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.These approaches are essential for understanding how candidate drug targets function within complex biological systems.<h5>Sample Preparation Challenges in Biological Research</h5>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.<h5>Advances in Chromatography and MS for Malaria Research</h5>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.<h5>Malaria, Drug Resistance, and Global Impact</h5>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.<h5>Advice for Early-Career Researchers</h5>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.<h5>Closing Remarks</h5>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

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The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

Phenomenex Kinetex Core-Shell HILIC Preparative HPLC Columns

Kinetex HILIC is a core-shell particle with unbonded silica, providing selectivity for retaining and separating polar compounds in HILIC chromatography.

Hydrophilic Polar Selectivity and Performance!The Kinetex HILIC is a core shell based particle with a unbonded silica phase is used under hydrophilic interaction chromatography conditions to provide the selectivity needed for the retention and separation of polar compounds. This versatile column can easily upgrade existing fully porous 5 µm and 3 µm methods to shorten run time, increase sensitivity, and even provide greater resolution with much higher efficiency levels.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems</li><li>Unique Polar Retentive L3 Silica</li><li>Unbonded Silica Phase</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_Core_Shell_HILIC_HPLC_Column_9ff82b0e20.webp" alt="Phenomenex: Kinetex Core-Shell HILIC HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_Core_Shell_HILIC_HPLC_Column_9ff82b0e20.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 0 %</li><li>Particle Size (μm): 5</li><li>Recommended Use: Retention and separation of polar compounds hydrophilic interaction chromatography conditions</li></ul><h4>Alternative Polar Selectivity to a C18 &nbsp;</h4>A Core-Shell based silica phase that combines high-performance with novel polar HILIC selectivity.<h4>Increase Polar Retention</h4>Used under HILIC running conditions, this phase provides the highest polar selectivity for retention and separation of hydrophilic compounds.<blockquote>Stationary phase Kinetex Core-Shell HILIC is provided in these column types: analytical and preparative</blockquote>Axia Packed Preparative Columns<ul><li>Column length (mm): 100, 150, 250</li><li>Column ID (mm): 21.2</li><li>Particle size (µm): 5</li></ul><h3>Kinetex Core-Shell Technology Columns</h3>Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media, which can be leveraged to increase resolution, significantly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.Advantages of Kinetex HPLC Columns<ul><li>Performance gains on ANY liquid chromatography system</li><li>System-to-system and lab-to-lab method portability</li><li>Improve the productivity of older, established methods.</li></ul><h4>How Does Core-Shell Technology Work?</h4>Core-shell technology in chromatography is a revolutionary approach that enhances the efficiency of HPLC systems while minimizing costs. Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for faster analyte mass transfer between the mobile and stationary phases, as diffusion occurs only through the thin, porous shell rather than the entire particle. This minimizes band broadening, leading to higher separation efficiency and sharper peaks.The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.<h3>Kinetex Core-Shell Performance on Any LC System</h3>The 5 versatile particle sizes allow you to select the best fit for your instrument.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"><h3>Particle Chemistry</h3>Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.<ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul>Kinetex particles are characterized by an exceptionally consistent size distribution, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to enhanced separation efficiency and sharper peaks.The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"><h3>Core-shell vs Fully Porous</h3><h4>High-Efficiency‚ High-Density Particle</h4>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"><h3>Expand Your Core-Shell Selectivity Toolbox</h3>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of 11 diverse stationary phases, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.<h3>Top 5 Pros of Kinetex Core-Shell Technology</h3><ul><li>Productivity: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li>Portability The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li>Performance Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li>Phases With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li>Pressure Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Phenomenex Lux Cellulose-1 Chiral HPLC Columns

Phenomenex Lux Cellulose-1 chiral HPLC column offers reliable enantiomer separation, matching or exceeding CHIRALCEL® OD® performance across multiple modes.

This universally trusted cellulose phenyl carbamate derivative is absolutely essential to any chiral screen. Lux Cellulose-1 is a guaranteed alternative to CHIRALCEL® OD® and OD-HX. Expect equivalent or better performance when using this Lux phase.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Cellulose_1_Chiral_HPLC_Columns_f70a9f21a0.jpg" alt="Phenomenex: Lux Cellulose-1" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Cellulose_1_Chiral_HPLC_Columns_f70a9f21a0.jpg 245w," sizes="100vw" width="500" height="150"><ul><li>Separation Mode: Chiral</li><li>Phase Composition: Regenerated Cellulose (RC)</li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.</li><li>Stationary phase: Cellulose tris(3,5-dimethylphenylcarbamate)</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux Cellulose-1 columns are guaranteed to perform as well or better than the equivalent CHIRALCEL® OD® and OD-H® Chiral Technologies column. Lux phases can also provide alternative selectivity to other chiral selectors for the most challenging chiral separations. Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects.The helical structure of the cellulose creates a complex steric environment that provides stronger interactions and better chiral recognition abilities. Additionally, the selector’s constituents produce hydrogen bonding, dipole, and π-π interactions that all affect the CSPs ability to separate your enantiomers effectively.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Performance_Advantage_f239557883.jpg" alt="Phenomenex: Lux Performance Advantage" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Performance_Advantage_f239557883.jpg 182w," sizes="100vw" width="677" height="580">

Phenomenex Lux Cellulose-2 Chiral HPLC Columns

Phenomenex Lux Cellulose-2 chiral HPLC column, a halogenated cellulose phase, ensures superior enantiomer separation and is a proven alternative to CHIRALCEL® OZ®.

This first to market halogenated cellulose phenyl carbamate derivative offers unique chiral recognition abilities that complement the rest of the Lux family of columns.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomene_Lux_Cellulose_2_Chiral_HPLC_Columns_4aa21cccda.jpg" alt="Phenomenex: Lux Cellulose-2 Chiral HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomene_Lux_Cellulose_2_Chiral_HPLC_Columns_4aa21cccda.jpg 245w," sizes="100vw" width="768" height="438"><ul><li>Separation Mode: Chiral</li><li>Stationary phase: Regenerated Cellulose (RC)<ul><li>Cellulose tris(3-chloro-4-methylphenylcarbamate)</li></ul></li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux Cellulose-2 columns are guaranteed to perform as well or better than the equivalent CHIRALCEL-OZ and OZ-H® Chiral Technologies column. Lux phases can also provide alternative selectivity to other chiral selectors for the most challenging chiral separations.Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects. The helical structure of the cellulose creates a complex steric environment that provides stronger interactions and better chiral recognition abilities. Additionally, the selector’s constituents produce hydrogen bonding, dipole, and π-π interactions that all affect the CSPs ability to separate your enantiomers effectively.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Performance_Advantage_cellulose_2_bbd747a32c.jpg" alt="Phenomenex: Lux Performance Advantage" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Performance_Advantage_cellulose_2_bbd747a32c.jpg 179w," sizes="100vw" width="677" height="590">

Phenomenex Lux Cellulose-3 Chiral HPLC Columns

Phenomenex Lux Cellulose-3 chiral HPLC column provides reliable enantiomer separation, performing as well or better than CHIRALCEL® OJ® with unique chiral selectivity.

This first to market halogenated cellulose phenyl carbamate derivative offers unique chiral recognition abilities that complement the rest of the Lux family of columns.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Cellulose_3_Chiral_HPLC_Columns_2_74f7d52275.jpg" alt="Phenomenex: Lux Cellulose-3 Chiral HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Cellulose_3_Chiral_HPLC_Columns_2_74f7d52275.jpg 216w," sizes="100vw" width="903" height="650"><ul><li>Separation Mode: Chiral</li><li>Stationary phase: Regenerated Cellulose (RC)<ul><li>Cellulose tris(4-methylbenzoate)</li></ul></li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux Cellulose-3 columns are guaranteed to perform as well or better than the equivalent CHIRALCEL® OJ® and OJ-H® Chiral Technologies column. Lux phases can also provide alternative selectivity to other chiral selectors for the most challenging chiral separations. Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects.The helical structure of the cellulose creates a complex steric environment that provides stronger interactions and better chiral recognition abilities. Additionally, the selector’s constituents produce hydrogen bonding, dipole, and π π interactions that all affect the CSPs ability to separate your enantiomers effectively.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Performance_Advantage_cellulose_3_a232dc359a.jpg" alt="Phenomenex: Lux Cellulose-3 Performance Advantage" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Performance_Advantage_cellulose_3_a232dc359a.jpg 179w," sizes="100vw" width="677" height="590">

Phenomenex Lux i-Cellulose-5 Chiral HPLC Columns

Phenomenex Lux i-Cellulose-5 chiral HPLC column delivers broad enantioselectivity, strong solvent compatibility, and scalable performance for LC and SFC separations.

<h4>Your New First Choice Chiral Column!</h4>The immobilization and bonding technology used within the Lux i-Cellulose-5 promotes column stability in strong organic solvents, which affords you the ability to expand your chiral separation success with more solvent systems and separation modes.<ul><li>Wide Solvent Flexibility</li><li>Broad Enantioselectivity</li><li>Strong Solvent Compatibility</li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, immobilized with a polysaccharide stationary phase. This Immobilization provides additional strong organic solvent ruggedness so that DCM, DMSO and other related strong solvents can be used on column.</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux® i-Cellulose-5 contains achemical crosslinking between the polysaccharide and silica supports which inturn provides incredible strong solvent (THF, DCM, DMSO, MtBE, Ethyl Acetate, etc.) robustness compared to coated phases and prevents the phase from being dissolved in strong solvents.Lower pressure, all purpose particle size for analytical LC (HPLC) or SFC methods and especially for high performance preparative LC or SFC puriﬁcation work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_i_Cellulose_5_Chiral_HPLC_Columns_28bc99dbb1.jpg" alt="Phenomenex: Lux i-Cellulose-5 Chiral HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_i_Cellulose_5_Chiral_HPLC_Columns_28bc99dbb1.jpg 245w," sizes="100vw" width="1541" height="595"><h4>Dependability and Seamless Scalability</h4>Our highest standards of quality will ensure that you are fully satisfied with each and every Lux chiral column as consistent quantitation and results come with every Lux batch and column that we manufacture. With matching selectivity, the 3 μm and 5 μm i-Cellulose-5 particle sizes allow you to scale down to increase resolution or easily scale up for preparative purification work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Incredible_consistency_batch_to_batch_and_between_particle_sizes_4a8adc47b8.jpg" alt="Phenomenex: Incredible consistency batch-to-batch and between particle sizes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Incredible_consistency_batch_to_batch_and_between_particle_sizes_4a8adc47b8.jpg 103w," sizes="100vw" width="677" height="1025">

Phenomenex Lux Amylose-1 Chiral HPLC Columns

Phenomenex Lux Amylose-1 chiral HPLC column ensures reliable enantiomer separation, matching or exceeding CHIRALPAK® AD® with strong recognition and broad selectivity.

This universally trusted amylose phenylcarbamate derivative is absolutely essential to any chiral screen. Lux Amylose-1 is a guaranteed alternative to CHIRALPAK® AD®. Expect equivalent or better performance when using this Lux phase.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Amylose_1_Chiral_HPLC_Columns_a5e2773084.jpg" alt="Phenomenex: Lux Amylose-1 Chiral HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Amylose_1_Chiral_HPLC_Columns_a5e2773084.jpg 245w," sizes="100vw" width="1236" height="626"><ul><li>Stationary phase: Amylose tris(3,5-dimethylphenylcarbamate)</li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux columns are guaranteed to perform as well or better than the equivalent DAICEL® Chiral Technologies column. Lux phases can also provide alternative selectivity to other chiral selectors for the most challenging chiral separations. Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects.The helical structure of the amylose creates a complex steric environment that provides stronger interactions and better chiral recognition abilities. Additionally, the selector’s constituents produce hydrogen bonding, dipole, and π π interactions that all affect the CSPs ability to separate your enantiomers effectively.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Amylose_1_separation_c517f7e910.jpg" alt="Phenomenex: Separation comparison - Lux Amylose-1 vs. CHIRALPAK AD-H" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Amylose_1_separation_c517f7e910.jpg 179w," sizes="100vw" width="677" height="590">

Phenomenex Lux i-Amylose-3 Chiral HPLC Columns

Phenomenex Lux i-Amylose-3 chiral HPLC column offers broad enantioselectivity, strong solvent stability, and proven performance as an alternative to CHIRALPAK® IG®.

<h4>Your New First Choice Chiral Column!</h4>The immobilization and bonding technology used within the Lux i-Amylose-3 promotes column stability in strong organic solvents, which affords you the ability to expand your chiral separation success with more solvent systems and separation modes.<ul><li>Wide Solvent Flexibility</li><li>Broad Enantioselectivity</li><li>Strong Solvent Compatibility</li><li>Recommended Use: Separation of enantiomers and racemic mixtures with strong stability in strong organic solvents</li></ul><h4>Achieve Greater Chiral Separation Success</h4>The Lux i-Amylose-3, like the rest of the Lux product line, is an incredibly versatile phase that has been manufactured and developed to give you your desired results again and again. This is especially true under harsh conditions where coated phases are unable to perform.Lower pressure, all purpose particle size for analytical LC (HPLC) or SFC methods and especially for high performance preparative LC or SFC puriﬁcation work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_i_Cellulose_5_Chiral_HPLC_Columns_28bc99dbb1.jpg" alt="Phenomenex: Lux i-Amylose-3 Chiral HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_i_Cellulose_5_Chiral_HPLC_Columns_28bc99dbb1.jpg 245w," sizes="100vw" width="1541" height="595"><h4>Guaranteed Alternative to CHIRALPAK® IG®</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Guaranteed_Alternative_to_CHIRALPAK_IG_584996e87b.jpg" alt="Phenomenex: Guaranteed Alternative to CHIRALPAK® IG®" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Guaranteed_Alternative_to_CHIRALPAK_IG_584996e87b.jpg 176w," sizes="100vw" width="677" height="600">

Phenomenex Strata X-AW Solid Phase Extraction (SPE)

Strata-X-AW mixed-mode SPE sorbent enables strong retention of acids (pKa ≤5) with 100% organic wash, ensuring maximum removal of interferences for clean, reliable extractions.

Weak Anion Mixed-Mode Solid Phase Extraction (SPE)A weak anion-exchange functionalized polymeric sorbent that allows for complete retention of acidic compounds with a pKa less than 5, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_AW_SPE_cd5908db4d.jpg" alt="Phenomenex: Strata-X-AW SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_AW_SPE_cd5908db4d.jpg 245w," sizes="100vw" width="500" height="268"><ul><li>Recommended Use: Extraction of acidic compounds, particularly strong acids</li></ul><h4>Starting Method</h4><h5>For Acids with pKa ≤ 5</h5><ul><li>Column: 1 mL Methanol</li><li>Equilibrate: 1 mL Water, pH 6-7</li><li>Load: Diluted Sample pH 6-7</li><li>Wash 1: 1 mL 25 mM Ammonium Acetate Buffered, pH 6-7</li><li>Wash 2: 1 mL Methanol</li><li>Elute Any Acid: 2x 500 µL 5 % NH4OH in Methanol</li><li>Elute Weak Acids: 2x 500 µL 5 % Formic Acid in Methanol</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080">Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.<h4>Available Formats</h4><ul><li>96-Well-Plate</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>1, 3, and 6mL Tubes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>Giga Tubes (12-150mL Tubes)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080">

Phenomenex Strata X-A Solid Phase Extraction (SPE)

Strata-X-A strong anion-exchange SPE retains weak acids (pKa >2) with 100% organic wash, maximizing interference removal. Available in tubes, plates, and giga formats.

Strong Anion Mixed-Mode Solid Phase Extraction (SPE)A strong anion-exchange functionalized polymeric sorbent that allows for complete retention of weakly acidic compounds with a pKa greater than 2, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_A_SPE_e107f0b6bf.jpg" alt="Phenomenex: Strata-X-A SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_A_SPE_e107f0b6bf.jpg 245w," sizes="100vw" width="500" height="268"><ul><li>Recommended Use: Extraction of weakly acidic compounds</li></ul><h4>Starting Method</h4><h5>For Acids with pKa &gt; 2</h5><ul><li>Column: 1 mL Methanol</li><li>Equilibrate: 1 mL Water, pH 6-7</li><li>Load: Diluted Sample pH 6-7</li><li>Wash 1: 1 mL 25 mM Ammonium Acetate Buffered, pH 6-7</li><li>Wash 2: 1 mL Methanol (collect this fraction to analyze Neutral/Bases)</li><li>Elute Acid: 2x 500 µL 5 % Formic Acid in Methanol</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080">Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.<h4>Available Formats</h4><ul><li>96-Well-Plate</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>1, 3, and 6mL Tubes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>Giga Tubes (12-150mL Tubes)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080">

Phenomenex Strata X-C Solid Phase Extraction (SPE)

Strata-X-C strong cation-exchange SPE retains basic compounds (pKa ≤10.5) with 100% organic wash for maximum cleanup. Offered in tubes, plates, and giga formats.

Strong Cation Mixed-Mode Solid Phase Extraction (SPE)A strong cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pKa less than 10.5, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_C_SPE_4927d7340d.jpg" alt="Phenomenex: Strata-X-C SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_C_SPE_4927d7340d.jpg 245w," sizes="100vw" width="500" height="268"><ul><li>Recommended Use: Extraction of weakly basic compounds</li></ul><h4>Starting Method</h4><h5>For Bases with pKa ≤ 10.5</h5><ul><li>Column: 1 mL Methanol</li><li>Equilibrate: 1 mL Acidified Water</li><li>Load: Diluted Acidified Sample</li><li>Wash 1: 1 mL 0.1 N HCI in Water (collect this fraction to analyze Polar Neutrals)</li><li>Wash 2: 1 mL 0.1 N HCI in Methanol (collect this fraction to analyze Neutrals/Acids)</li><li>Elute Bases: 2x 500 µL 5 % NH4OH in Methanol</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080">Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.<h4>Available Formats</h4><ul><li>96-Well-Plate</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>1, 3, and 6mL Tubes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>Giga Tubes (12-150mL Tubes)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080">

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