<h3>A Dedicated Solution for Oligonucleotide Analysis</h3>Oligonucleotides have become key players in modern pharmaceutical and biological research, particularly in the development of highly targeted and personalized therapies. As increasing numbers of oligonucleotide-based drugs enter clinical trials or receive regulatory approval, analytical laboratories face growing demands for both throughput and data quality. These molecules present unique chromatographic challenges due to their long, highly polar structures and subtle variations in nucleobase sequence or functionalization.<h3>Sepapure oliGO – Designed for Oligonucleotide Separations</h3>Selecting the optimal chromatographic approach for oligonucleotide analysis can be complex. Many laboratories rely on ion-pair reversed-phase (IP-RP) chromatography because of its speed, robustness, and compatibility with mass spectrometry. However, commonly used ion-pair reagents can degrade conventional silica-based stationary phases.<a target="_blank" rel="noopener noreferrer" href="https://www.knauer.net/columns-oligonucleotide-columns">Sepapure oliGO</a> addresses this limitation through a hybrid silica matrix combined with specialized surface end-capping, ensuring resistance to aggressive IP-RP reagents. Its functionalized surface enables both strong reversed-phase interactions and controlled ionic contributions, effectively creating a mixed-mode separation mechanism. This design results in improved durability compared with standard mixed-mode columns, making Sepapure oliGO a robust choice for routine oligonucleotide workflows.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Sepapure_oli_GO_columns_with_laser_etched_column_information_23f545be81.jpg" alt="KNAUER: Figure 1 - Sepapure oliGO columns with laser-etched column information." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Sepapure_oli_GO_columns_with_laser_etched_column_information_23f545be81.jpg 223w," sizes="100vw" width="1186" height="828"><h3>Method Transfer and Comparative Performance</h3>In collaboration with BianoGMP GmbH, KNAUER’s application team evaluated Sepapure oliGO against a widely used reference column. The study compared oligonucleotides of varying lengths and purities, including a fully thiolated 43-mer DNA. Particular attention was paid to potential interactions between thiolated oligonucleotides and stainless-steel hardware, which have been reported to cause peak tailing.The results demonstrated that direct method transfer between the reference column and Sepapure oliGO was generally feasible without significant adjustment. Chromatographic resolution remained comparable, while retention times were slightly shorter on Sepapure oliGO. Furthermore, the anticipated benefits of expensive bioinert hardware were not confirmed under the tested conditions, suggesting that such effects may primarily influence very fast (U)HPLC separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Overlayed_chromatogram_of_crude_product_of_a_43mer_thiolate_oligonucleotide_7ae9613efd.jpg" alt="KNAUER: Figure 2: Overlayed chromatogram of crude product of a 43mer thiolate oligonucleotide. Light blue - reference column and dark blue – Sepapure oliGO." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Overlayed_chromatogram_of_crude_product_of_a_43mer_thiolate_oligonucleotide_7ae9613efd.jpg 245w," sizes="100vw" width="1223" height="453"><h3>Low Backpressure for Reliable Operation</h3>Sepapure oliGO can be applied effectively in both HPLC and (U)HPLC workflows. Its low operating backpressure minimizes risks associated with clogging, excessive system wear, and performance deterioration over time. Typical IP-RP separations using 3 µm particles generated pressures around 200 bar, enabling laboratories without ultra-high-pressure systems to perform high-quality oligonucleotide analysis.<h3>Best Practices for Column Performance</h3>To maintain optimal performance, users should observe recommended operating conditions, including a broad pH range of 1.5–10 and temperatures up to 60 °C. Employing a guard column can prevent coarse synthesis by-products from reaching the analytical column. If pressure increases are observed, backflushing at low flow rates can restore performance.When the system is idle, thorough flushing to remove salts is essential. For storage, the column should be kept in a 1:1 mixture of methanol–water or acetonitrile–water. Gradient transitions are recommended when switching between standard RP eluents and IP-RP reagents. Regeneration procedures developed for RP columns are also suitable for Sepapure oliGO.<h3>Beyond Analytical Separations</h3>For large-scale purification, ion-exchange chromatography (AEX) may offer advantages. Scalable preparative systems are available to support purification workflows, ranging from compact laboratory setups to pilot-scale platforms with flow rates up to 800 mL/min. Bioinert configurations can be integrated where required, allowing laboratories to tailor purification solutions to their specific oligonucleotide applications.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at sales@knauer.net. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at folmert@knauer.net&nbsp;</li></ul></blockquote>

Oligos Made Easy - Part 8: Sepapure oliGO

Visit the LabRulez booth at <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/871">analytica 2026</a> in Munich, 24. - 27. March 2026! You can find us in Hall A1, Booth A1.506. At the booth, you will be able to explore live demonstrations of how the LabRulez portals work and see selected analytical instruments from our pre-owned marketplace section.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg" alt="LabRulez at Analytica 2026: Hall A1 Floor Plan and Booth Location" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg 245w," sizes="100vw" width="1516" height="881"><h3>Would you like to learn how the LabRulez portals work?</h3>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/">LabRulezLCMS</a> ➡︎ <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/">LabRulezGCMS</a> ➡︎ <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/">LabRulezICPMS</a><ul><li>A unique online solution for your marketing</li><li>A targeted audience from the analytical chemistry community</li><li>Modern technologies and a unique concept</li><li>Design optimized for most smart devices – PC, mobile, and tablet</li><li>Advanced statistical tools fully available to users</li></ul><h3>Services Offered</h3><h4>News + Company Updates</h4><ul><li>Publish news related to your instrumentation, applications, services, events, job openings, and more</li><li>News is shared both on our portals and across our social media channels</li></ul><h4>Library – All Documents in One Place</h4><ul><li>Applications, posters, presentations, technical articles, brochures, specifications</li><li>Fast and advanced search capabilities</li><li>Over 32,000 documents currently available in our database</li></ul><h4>Webinars</h4><ul><li>Upcoming and on-demand webinars from the field of analytical chemistry</li><li>More than 5,500 webinars covering chromatography, mass spectrometry, and spectroscopy</li></ul><h4>Careers</h4><ul><li>Targeted job listings in analytical chemistry</li><li>Clear filtering by specialization or location</li><li>Increased visibility for job postings</li></ul><h4>Companies</h4><ul><li>Company profile with key information and contacts</li><li>Integration of all your activities on our portal: News, Library documents, Webinars, Upcoming Events, Careers, and mentions by partners or customers</li><li>Connection to your Products listed on LabRulez portals</li></ul><h4>Products and Services</h4><ul><li>Comprehensive overview of instrumental techniques and services</li><li>Targeting the analytical chemistry community and customers in the growing online environment</li></ul><h4>Marketplace</h4><ul><li>Buying and selling pre-owned analytical equipment</li><li>We purchase analytical instrumentation of all brands and ages</li><li>Sell your equipment through LabRulez</li><li>Request and purchase complete systems, modules, or spare parts with warranty and service</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg" alt="LabRulez at analytica 2026" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg 88w," sizes="100vw" width="1600" height="2844"><h3>What Will Be on Display at Our Booth?</h3>Come and explore selected spare parts and modules from our marketplace section. These include both new, tested, and pre-owned items.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar">LabRulezLCMS Marketplace</a></h4>Spare parts for LC/MS:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=378">Vacuum turbo pumps for LC/MS</a></li></ul>Spare parts for HPLC:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=397">Degassers for HPLC/UHPLC</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=368&amp;search=valve">Automatic valves for HPLC</a></li><li>HPLC components for Agilent 1100, 1200, and 1260 Infinity II series</li></ul>Consumables:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=377">HPLC lamps</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=48">HPLC columns</a></li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar">LabRulezGCMS Marketplace</a></h4>Spare parts for GC/MS:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=367&amp;search=turbo">Turbo pumps for GC/MS</a></li></ul>Spare parts for GC:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=366&amp;search=EPC">EPC modules</a></li></ul>Sample injection<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar/432">Autosampler 7693A for GC</a></li></ul>Consumables:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=7">GC columns </a>and selected accessories</li></ul>

LabRulez at analytica 2026

News from LabRulezLCMS Library - Week 13, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Separation Science

Agilent Technologies

Shimadzu Corporation

Waters Corporation

With the worldwide growth in the oligonucleotide markets, applying the appropriate tools for oligonucleotide analysis and purification is essential. Ion-paired reverse-phase (IP-RP) liquid chromatography involves the use of ion-pairing reagents to form neutral, hydrophobic complexes with charged oligonucleotides, facilitating their retention and separation on reversed phase columns.This presentation introduces the principles and applications of IP-RP in oligonucleotide analysis. It will cover key factors influencing the efficiency of IP-RP methods such as column chemistry, choice of ion-pairing reagent, and mobile phase composition. Additionally, we will discuss method considerations for scaling up your oligonucleotide purification method.<blockquote>Presenter: Melissa Goodlad (Application Engineer, Agilent Technologies, Inc.)Melissa Goodlad is an Application Engineer providing applications assistance and technical support for Agilent’s HPLC columns and consumables. Before joining Agilent, Melissa was completing her PhD in Physical Chemistry at UC Merced studying nanoparticle self-assemblies.</blockquote>

Agilent Technologies: Becoming a Better Chromatographer - Educational Webinar Series

An Introduction to Ion-Paired Reverse-Phase Oligonucleotide Separations: From Analysis to Purification

This presentation introduces the principles and applications of IP-RP in oligonucleotide analysis.

An Introduction to IP-RP Oligonucleotide Separations

<div><div>Let's go back to the Basics of Chromatography, Mass Spectrometry, Data Analysis and Trouble-shooting: understanding the importance of the basics will enable you to be more effective in your daily laboratory life.This journey started in 2024 and we are now pleased to invite you to join us to go deeper in fundamentals, troubleshooting and application specific topics.<h3>EPISODE 3: Fit for Purpose Analysis: How to Develop a Method for Challenging Separations</h3>What will you learn?<ul><li>Predict the Molecule's Retention in LC</li><li>Select the Critical Parameters during Method Development</li></ul>What's next?<ul><li>Check out the full Webinar Series Episodes List <a target="_blank" rel="noopener noreferrer" href="https://event.on24.com/eventRegistration/EventLobbyServlet?target=reg20.jsp&amp;eventid=4817889&amp;sessionid=1&amp;key=B9182AE813B3F8A30217606156F96B75&amp;groupId=5901784&amp;sourcepage=register">here</a></li></ul>Not sure to be able to attend live sessions?&nbsp;<ul><li>Register today and watch the webinars on-demand</li></ul><blockquote>Presenter: Tobias Langrock (Senior Application Specialist Chemistry, Europe Waters Corporation)During his time as a PhD student Tobias discovered his passion for LC and LC-MS/MS and has not lost it since. Joining Waters in 2008 was the perfect opportunity to expand his understanding and expertise in chromatography and mass spectrometry. Since then, he has been involved with a very wide range of instruments and applications, whether small or large molecules, reversed phase LC, IEX or SEC, SFC or GPC. His focus is always on the customers and their daily chromatographic challenges.Presenter: Andrea Gheduzzi (Principal LC Market Dev. Manager, EMEA, Waters Corporation)Andrea Gheduzzi, is a principal LC market development manager for EMEA at Waters Corporation. Andrea Gheduzzi has spent more than 30 years in the chromatography field. Across his career he had various roles, service engineer, chromatography lab manager position, and technical manager for chromatography columns. He has mainly been dealing with pharma accounts, also supporting LC method development activities using DoE/QbD approaches. In his previous role as Pharma Business Development Manager at Waters he has further expanded his expertise in AQbD and related applications. Since 2023 he became principal LC market development manager for EMEA and member of the AQbD working party at the EDQM.Presenter: Arjan Timmerman (Pharma Market Development Manager EMEA, Waters Corporation)Arjan Timmerman is a market development manager at Waters working in EMEA. Prior to joining Waters in 2014 he worked for 17 years as an Analytical Scientist in the Small Molecule Pharmaceutical industry in various Chemical and Pharmaceutical late-stage development labs. Since 2023 he became QA/QC market development manager for EMEA working in a dedicated team and focuses on Pharmaceutical workflows, AQbD, Impurities, LC Chromatography and Compliance.</blockquote></div></div>

Waters Corporation: Back to Basics Advanced Webinar Series 2025

Back to Basics Advanced - Pharma Impurities: Separations

Predict the Molecule's Retention in LC. Select the Critical Parameters during Method Development.

<div><div><h3>Oligos, Oligos, Oligos!</h3>Oligonucleotide therapeutics and reagents are everywhere. They are helping the world address previously untreatable diseases, and they are also being used in new ways to sensitively quantify and sequence important translational research samples. Whether it be a CRISPR guide RNA, lipid conjugated anti-sense oligonucleotide, or a specially modified assay probe, these molecules must be tested to ensure they have the right sequence, pinpoint-specific modifications, conjugate moieties and purities. &nbsp;And when dosed as a therapeutic, they need to be carefully quantified to confirm pharmacokinetic models. &nbsp;Join the Waters Chromatography and LC-MS Team and their collaborators from MRIGlobal, Regeneron, and Acuitas Therapeutics in this special episodic, 3 week webinar series to learn more about the science of oligos and the power of LC-based approaches to analyze them.&nbsp;<h3>EPISODE 3</h3><h4>Part 1: Deformulation and Nucleic Acid Quantification in LNPs Using Size-Exclusion Chromatography</h4>Learn about an alternative high-throughput approach for the deformulation and quantification of nucleic acid payloads in lipid nanoparticles (LNPs). Using a low adsorption 2.5 µm SEC 450 Å Column, this size-exclusion chromatography (SEC) method employs deformulating conditions with detergent and organic solvents in the mobile phase to disrupt LNPs without prior sample treatment.<blockquote>Presenter: Mateusz Imiolek, PhD (Waters Corporation, Geneva, Switzerland)Mateusz joined Waters in 2022 where he has been studying challenges associated with characterizing gene therapeutics and ensuring that new Waters products are fit for this purpose. Before that, he completed his academic training in bioorganic chemistry, which allows him to apply detailed molecular insights to ensure the newly developed columns and assays are carefully designed for such analytes.</blockquote><h4>Part 2: High-Throughput Nucleic Acid and Lipid Adduct Quantification in LNPs Using Ion-Paired Reverse-Phase Chromatography</h4>A high-throughput LC-based assay developed for nucleic acid quantification in lipid nanoparticles (LNPs). By using a short, low adsorption 2.5 µm BEH C18 column, the assay allows for the efficient separation of LNP payloads, including guide RNA and mRNA, from detergent-disrupted samples. The assay is designed for excellent robustness and reproducibility, making it suitable for routine application.<blockquote>Presenter: Razvan Cojocaru (Research Scientist II, Acuitas Therapeutics)Razvan has been a research scientist in the Analytical department at Acuitas Therapeutics since 2021, where he focuses on developing new methodology for characterizing nucleic acids in LNP therapeutics. Prior to Acuitas, Razvan completed his studies at Simon Fraser University in Molecular Biology and Biochemistry, where he focused on Ribozyme and RNA Aptamer in vitro selection and optimization.</blockquote></div></div>

LC for LNP RNA Molecular Composition Analysis

Join Waters Chromatography, LC-MS Team & collaborators for a 3-week webinar series on oligos science & LC-based analysis.

Polysaccharide vaccines play a critical role in preventing diseases caused by encapsulated bacteria, such as Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis. These vaccines are in complex polysaccharide protein conjugate molecules that stimulate the immune system and provide long-lasting protection.&nbsp;In this webinar, we will explore analytical techniques used to evaluate the efficacy, safety, and quality of polysaccharide vaccines.Key learning objectives:<ul><li>Understand regulatory perspectives and the resources provided by USP for the characterization of polysaccharide vaccines</li><li>Explore the benefits of LC coupled with Multi-Angle Light Scattering (MALS) as a quality attribute</li></ul>Who should attend:<ul><li>Analytical scientists involved with the characterization of polysaccharide vaccines.</li></ul><blockquote>Presenter: Mandy Alger (Senior Scientist II, Global Biologics US Pharmacopeia)Mandy has been working on biopharmaceuticals and vaccine development for more than twenty years. Prior to her tenure at the United States Pharmacopeia, she was employed by organizations like GlaxoSmithKline (GSK), the National Institutes of Health (NIH), and AstraZeneca (AZ).Joined the Global Biologics Division of USP in 2021 and currently serves as a Senior Scientist II at USP. Collaborates with the USP teams, the pharmaceutical industry, and the FDA to develop USP reference standards and documentation standards pertaining to pharmaceuticals, includingcomplex Proteins, Heparin, Dextran, Oxytocin, Oligosaccharide, and polysaccharide vaccine molecular weight standards.Presenter:&nbsp;Katayoun Morakabi, Ph.D. (Application Scientist Waters l Wyatt Technology)Katayoun Morakabi is an application scientist in the Analytical Sciences team at Waters | Wyatt Technology. She assists customers in identifying the best light scattering solutions for their analytical needs by leveraging her expertise in multi-angle light scattering. She performs feasibility studies to characterize customer samples and participates in Wyatt’s Light Scattering University. She completed her Ph.D. in Biochemistry from the University of Texas at Dallas. Her doctoral work focused on characterizing the structure, dynamics, and functional outcomes of histone acetyltransferases and histone chaperone proteins.Moderator: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.</blockquote>

Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

Polysaccharide Vaccines

In this webinar, we will explore analytical techniques used to evaluate the efficacy, safety, and quality of polysaccharide vaccines.

Oligonucleotides are highly important biopharmaceuticals with strongly increasing relevance. Due to the high similarity of impurities originating from the synthesis, however, purification can be challenging.This seminar demonstrates a successful large scale purification of an oligonucleotide using anion exchange chromatography (AEX) as well as efficient quality control by applying ion-pairing reversed phase chromatography (IP-RP).<h4>Content</h4><ul><li>How to perform a large scale AEX purification: Design of preparative system and useful features</li><li>Benefits and limitations using AEX for preparative purification</li><li>What to consider for developing an IP-RP methods: selection of stationary phase, ion-pair reagent, and solvent conditions</li><li>How to improve analytical results by utilizing bioinert column hardware</li></ul><h4>Who are the seminar for?</h4><ul><li>Advanced and beginners interested in peptides and/or oligonucleotides.</li></ul><blockquote>Presenter: Dr. Ulrike Krop (KNAUER Wissenschaftliche Geräte GmbH)Presenter: Dr. Daniel Eßer (YMC Europe GmbH)</blockquote>

KNAUER: Purification and quality control of oligonucleotides

Purification and quality control of oligonucleotides

This seminar demonstrates a successful large scale purification of an oligonucleotide using anion exchange chromatography (AEX).

Ensuring food safety and quality is increasingly challenging. Whether you're analyzing for pesticide residues, ensuring that label claims are correct and accurate, or profiling for food authenticity, Agilent works with you, helping you deliver what your customers demand.Agilent's food and beverage testing products and services keep you at the forefront of the latest trends, ahead of issues, and focused where you need to be – delivering highest quality and value to your customers.In this webinar you will learn:<ul><li>Latest trends and regulatory updates in food testing.</li><li>Agilent latest HPLC &amp; UHPLC series introduction.</li><li>Agilent application solutions for various important food quality parameters for food &amp; beverages testing.</li></ul>Who should attend?<ul><li>Managers &amp; Scientists from food testing labs, food processing companies, contract testing labs, government regulatory bodies, labs, etc.</li></ul><blockquote>Presenter: Dr. Lalitha R Gowda (Chief Scientist (Rtd), CSIR - Central Food Technological Research Institute)Dr. Gowda retired as Chief Scientist at CSIR-Central Food Technological Research Institute, Mysore in the year 2014. She obtained her Ph. D in Biochemistry from Baylor University, Texas USA and a Post-doctoral training at the Department of Biochemistry University of Cambridge, UK.Her professional experience as a research scientist, with specialization in the areas of structural biology of plant proteins, protein engineering and analytical biochemistry and food safety, including grass-root work, academic activities, consultation, and positions of institutional leadership spans over three and half decades. She has over 90 peer-reviewed research publications in journals of reputeShe has a long experience as advisor and consultant to the Government in regulatory developments around health and nutrition, food analysis, and harmonization of standards in biotechnology and food safety programs.Presenter:&nbsp;Mr. Nitin G (Product Specialist – Liquid Chromatography &amp; Dissolution, Liquid Phase Division, Agilent Technologies India Pvt. Ltd.)Nitin is an established professional in the analytical industry with over 13 years of experience. He has made significant contributions at Agilent Technologies, where he currently serves as Product Specialist for Liquid phase division supporting our esteemed customers to get the optimal technological solutions in liquid chromatography domain for their laboratory needs and application requisites. With expertise in analytical technologies of Liquid Chromatography and Dissolution testing, Nitin is engaged in various technical knowledge exchange platforms focusing technological advancements, automation of workflows, and lab efficiency enhancement.</blockquote>

Agilent Technologies: Unlock the Possibilities of Food Quality Testing Using Modern Analytical Techniques

Unlock the Possibilities of Food Quality Testing Using Modern Analytical Techniques

In this webinar you will learn the latest trends and regulatory updates in food testing and Agilent latest HPLC & UHPLC series introduction.

Food Quality Testing by Modern Analytical Techniques

Liquid chromatography (LC) is one of the most widely used analytical techniques in the world. With recent initiatives for greener and more sustainable science, the large amounts of hazardous solvent waste generated by analytical scale LC instrumentation have been a topic of interest. By reducing the inner diameter (ID) of the fluidic pathway to the capillary scale (&lt;0.5 mm), separations comparable to their analytical scale counterparts can be performed using several orders of magnitude less solvent (mL down to µL).&nbsp;This presentation will focus on advances in the instrumentation used in the field of capillary LC. Additionally, practical applications of capillary LC will be discussed, including pharmaceutical and illicit drug analysis, food/beverage analysis, and automated reaction monitoring. Large molecule separations will also be featured, including various CQA validations of monoclonal antibodies.<h5>By attending this presentation, you will:</h5><ul><li>Gain insights into the latest advancements in capillary LC</li><li>Understand the environmental benefits of capillary LC and how it can contribute to greener and more sustainable analytical practices</li><li>Discover practical applications of capillary LC</li><li>Explore how capillary LC is used for large molecule separations</li></ul><h5>Who Should View?</h5>Scientists, chemists, analytical researchers, lab directors and managers, VPs and directors of QC/QA, lab owners<blockquote>Presenter: Sam Foster (Ph.D. Student, Department of Chemistry &amp; Biochemistry, Rowan University)Sam Foster is a 5th year Ph.D. student from Rowan University working under Dr. James Grinias. His research has included works on comprehensive two-dimensional liquid chromatography at the capillary scale, novel methods for UV-controlled electrospray ionization, and open-source data acquisition software and hardware. After graduation, he plans to continue in the field, working on furthering capillary scale chromatographic instrumentation.</blockquote>

Separation Science: Compact Capillary Liquid Chromatography: Advances and Applications

Compact Capillary Liquid Chromatography: Advances and Applications

This presentation will focus on advances in the instrumentation used in the field of capillary LC. 

Compact Capillary Liquid Chromatography

Preparative chromatography has been an integral component of pharmaceutical discovery and development for the past fifty plus years. The past thirty-five years have seen great advances in small molecule purification. Purification techniques have advanced from those centered around large irregular particles operating under low pressure to more efficient high-performance technologies utilizing small spherical particles. Technologies now routinely used include preparative HPLC, SMB, and SFC. At the discovery scale, these advances allow routine and rapid chiral and achiral purification of the large numbers of molecules synthesized at this stage of pharmaceutical R&amp;D. For pharmaceutical development these advances allow chromatographic purifications using HPLC and SMB to be a standard tool in the process development toolbox. The large number of pharmaceutical chiral SMB operations at the manufacturing scale has shown that chromatography can be a cost-effective alternative to asymmetric synthesis or other resolution techniques.This presentation will discuss the critical role small molecule purification plays within a pharmaceutical company. Multiple real-life examples from ug to metric ton scale supporting Discovery through Manufacturing activities will be presented. In addition, the importance of personnel, facility, automation, and information systems for a successfully integrated purification platform will be discussed.<blockquote>Presenter: Larry Miller (Associate Director, Novartis)Larry Miller is an Associate Director in the Global Discovery Chemistry Group at Novartis in Cambridge, MA responsible for analytical and purification support for Discovery. He has spent his career performing small molecule achiral and chiral purifications at the mg to multi-kg scale using a variety of chromatographic techniques. Larry has over 40 peer-reviewed publications and over 45 presentations at scientific meetings. He also co-authored “Modern Supercritical Fluid Chromatography: Carbon Dioxide Containing Mobile Phases” with David Pinkston and Larry Taylor. Larry also serves as co-instructor for an SFC short course which has been held at numerous chromatography conferences in the United States, Europe, and Asia over the past fifteen years. In addition, Larry is the current president of the Green Chemistry Group, a not-for-profit group that organizes the annual SFC conference.</blockquote>

Agilent Technologies: Small Molecule Chromatographic Purification in Pharmaceutical R&D and Manufacturing

Small Molecule Chromatographic Purification in Pharmaceutical R&D and Manufacturing

This presentation will discuss the critical role small molecule purification plays within a pharmaceutical company.

Small Molecule Purification in Pharma R&D and Manufacturing

Join us for this event that will discuss how Shimadzu SFC technology is being used to drive innovation throughout the drug development process. &nbsp;We will examine the break-through use of SFC with LabSolutions MD AQbD method development software to model and optimize peptide separations. &nbsp;The topic of Exposed Polar Surface Area analysis by SFC will be discussed and how its application to cyclic peptide therapeutics speeds up drug candidate selection. &nbsp;We will share recent developments in SFC separations for oligonucleotides, along with other enabling solutions for micro-prep SFC (analytical scale fractionation), hybrid UHPLC-SFC systems, and more.We will host a Q&amp;A session after the presentation to address any questions or comments.<blockquote>Presenter: Brian Domanski (Strategic Collaboration Scientist, Shimadzu Scientific Instruments)Brian has over 10 years working to provide LC and SFC solutions for Pharma, as well as experience with a wide range of chromatography techniques including RP, NP, HILIC, IEX, SEC/GPC, and IC. His focus also includes scalable Informatics and Data Integrity solutions.Presenter: Kevin Wons (Market Manager - Pharma/BioPharma, Shimadzu Scientific Instruments)Kevin has recently joined Shimadzu as Market Manager for Pharma/BioPharma. He has spent the last 6 years at AstraZeneca in the Analytical Sciences Department for product and process characterization for biologics in the AstraZeneca pipeline. Kevin has applications experience with HPLC and LC-MS to do Tryptic Peptide Mapping, Intact Mass Analysis, Subunit Mass Analysis, and Oligosaccharide Profiling. For his last 3 years, he acted as the Glycan Subject Matter Expert (SME) while at AstraZeneca. His work experience also includes Maryland Psychiatric Research Center, where he prepared animal tissue samples such as Brain, Liver, Blood, and Placenta to measure kynurenine pathway metabolites on GC/MS and HPLC. He has a Master’s degree in Professional Studies (MPS) focused on Biotechnology which prepares science professionals to fill management and leadership roles.</blockquote>

Shimadzu: Innovations in SFC Applications and Technology for Pharma and Biopharma

Innovations in SFC Applications and Technology for Pharma and Biopharma

Join us for this event that will discuss how Shimadzu SFC technology is being used to drive innovation throughout the drug development process. 

Innovations in SFC Applications in (Bio)Pharma 

Whether you are working in standard analytical lab, where unknown impurities are the biggest challenge or you are working in discovery labs where you need all the possible isolates for your further characterization or you are working nutraceuticals, natural product extract or working in R&amp;D department, or peptide/ Oligonucleotide synthesis. You need the most flexible, efficient purification / preparative LC systems and columns for the best outcomes.Any scientists working on Preparative HPLC or Purification system have few of the following questions to be addressed:<ul><li>Struggling with accurate &amp; precise compound purification?</li><li>Do you get the best recoveries of your isolation compounds?</li><li>Are your purification systems future upgradable?</li><li>Are your purification systems tailor made to your needs?</li></ul>Explore how Agilent purification systems help you support purification from analytical and full-scale purification system.Join us to know the latest the developments in Agilent Purification / Preparative systems.<h4>Who Should Attend?</h4><ul><li>Scientists working on Preparative HPLC or Purification system</li><li>Lab Heads of Synthetic R&amp;D, CRO, CDMO&nbsp;</li><li>Lab Scientists working on Synthetic peptide or Oligonucleotide compounds and impurities isolation &nbsp;</li></ul><blockquote>Presenter: Mr. Sambasivarao Vattikuti (Pre-Sales Application Engineer, Agilent Technologies India Pvt. Ltd.)Sambasivarao holds a Master of Pharmacy degree from SRM University, Chennai, and has 15 years of industry experience. He spent a decade working in New Product Development and Analytical Research &amp; Development at leading pharmaceutical companies such as Syngene and MicroLabs. Currently, he serves as an Application Engineer at Agilent Technologies, specializing in Chromatography, Dissolution, and Laboratory Informatics, with five years of experience in this role. His areas of expertise include Chromatography, Quality by Design (QbD), and Purification techniques.</blockquote>

Agilent Technologies: Everyday Purification & Isolation with Confidence

Everyday Purification & Isolation with Confidence

Explore how Agilent purification systems help you support purification from analytical and full-scale purification system.

LCMS webinars focusing on HPLC - page 12