<p><strong>Tasty beer starts with knowing what’s in your hops. Explore how online SPE makes bitter acid analysis in hops faster and more reliable.</strong></p><h4><strong>How can you detect bitter acid profiles quickly, reliably, and more sustainably?</strong></h4><p>For craft brewers and beer lovers alike, a refreshing experience is all about flavor.</p><p>So how can you achieve the desired bitterness in your beer? The answer is not just about the recipe. Finding the right flavor profile starts with the bitter acids present in the starting hops material.</p><div><div><p>Hops (the heart of your favorite beer) are the source of the brew’s distinctive flavor tied to bitterness and aroma.</p><p>At the molecular level, bitterness is driven by <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0308814622016338">α- and β-bitter acids</a>, like humulones and lupulones, whose concentrations determine the final sensory profile of the beer (<strong>Figure 1</strong>).</p><p>Accurately characterizing these compounds in each batch allows brewers to unlock the full potential of every variety of hops.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 1. General chemical structure of hop bitter acids." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg 222w," sizes="100vw" width="700" height="493"></p><p>While conventional analytical methods rely on long, manual sample preparation steps and hazardous solvents, laboratories now strive for greener and more efficient workflows.</p><p>And moving from manual to online sample preparation is one of the easiest ways your lab can reduce waste and — most importantly — improve reliability with automation.</p><h4><strong>Key takeaways</strong></h4><ul><li>Online SPE reduces hands-on work, shortens turnaround time, and supports more sustainable methods by cutting solvent use and eliminating single-use cartridges.</li><li>Automated UHPLC workflows are easier for new users to execute, offering more reproducible quantifications of α- and β-bitter acids in hops.</li><li>Accurate bitter acid profiles help brewers distinguish hop varieties and control flavor with confidence.</li></ul><h4><strong>Comparing offline vs. online solid phase extraction for bitter acid HPLC analysis</strong></h4><p>If you’ve spent long hours in the lab preparing samples for hops analysis, you’re not alone.</p><p>Manual sample preparation often takes the most time in hops analysis, an area where online <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/chromatography-consumables/sample-prep.html">solid phase extraction</a> (SPE) can make a real difference. Here’s why.</p><p>Manual SPE has a lot of manual steps. Extraction. Cartridge loading. Washing. Elution. And sometimes solvent exchange or reduction. All before your actual analysis begins. This labor-intensive workflow can take up to an hour and depends strongly on operator skillsets, making reproducibility a challenge for new hires.</p><h4><strong>Online SPE analysis provides key benefits like:</strong></h4><ul><li>Hands-off workflow free of cartridges, manifolds, or vacuum steps.</li><li>Column washing and re-equilibration occur in parallel with separation.</li><li>SPE step is complete in just two minutes.</li><li>Minimizes operator-dependent variability.</li><li>Uses less than 8 mL of solvent.</li></ul><p>Let’s explore what this technique looks like in practice.</p><h4><strong>Online SPE for analyzing α- and β-bitter acids in hops standard solutions.</strong></h4><p>Changing your sample preparation from manual to automated isn’t complicated. With the right tools you can fully integrate online SPE with your UHPLC system (<strong>Figure 2</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 2. Schematic workflow for the determination of bitter acids in hops. The process involves clean up and instrumental analysis using the Thermo Scientific Vanquish Online SPE UHPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg 245w," sizes="100vw" width="700" height="323"></p><h4><strong>The process for analyzing a hops standard solution is straightforward:</strong></h4><ol><li>First, extract hop samples with ethanol (a greener alternative to toluene or diethyl ether in conventional methods).</li><li>Inject the extract directly into your instrument for analysis. Bitter acids retain<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1970"><strong> on the SPE column</strong></a> while matrix interferences that can comprise quantitation are removed.</li><li>Next, backflush the SPE column to transfer the analytes to the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/423"><strong> analytical column </strong></a>for separation. While this separation is taking place, the SPE column is washed and re-equilibrated in parallel, keeping the total run time to 15 minutes, with the separation of all six major bitter acids in a single analysis (<strong>Figure 3</strong>).</li></ol><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 3. UV chromatogram of hop bitter acids in standard solution (1-cohumulone, 2-humulone, 3-adhumulone, 4-colupulone, 5-lupulone, 6-adlupulone)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg 245w," sizes="100vw" width="700" height="347"></p><h4><strong>Online SPE for analyzing α- and β-bitter acids in real hops samples</strong></h4><p>Analytical labs need methods that are accurate and reliable for brewers to develop distinct and reproducible flavor profiles across batches.</p><p>To assess the applicability of online SPE UHPLC methods for hops analysis, we analyzed bitter acid profiles from four species — Sultana, Barbe Rouge, Azacca, and Sabro.</p><p>The concentration of α-acids dominated all samples, while each hops species exhibited distinct profiles in terms of α-acid and β-acid content, making them suitable for different brewing strategies (<strong>Figure 4</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 4. Bitter acid profiles in four different hop species, error bars display SD (n=6). To check method consistency, we measured each hops extract six times. Variation between measurements was low, with relative standard deviation below 3.5 % across all hops species." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg 245w," sizes="100vw" width="700" height="410"></p><ul><li>For testing labs, this process translates into a workflow that is easy to run, fast, and delivers reliable results without added complexity.</li><li>For craft brewers, reliability means clear, trustworthy insights into hops bitter acid profiles, for better hop selection and more precise control of bitterness and flavor in your beer.</li></ul><h4><strong>From carefully procured samples to tasty sips</strong></h4><p>Efficient UHPLC workflows support faster results, less solvent use, and reliable bitterness data for brewers. And online SPE turns those advantages into practical, day-to-day benefits for brewers and labs alike.</p><ul><li>Less hands-on skills required for lab analysts</li><li>Faster results from labs to brewers</li><li>A more sustainable way to work</li><li>Clear insights into hops flavor</li></ul><p>To learn more about the method and see the full application data, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_004238_hplc_vanquish_online_spe_bitter_acids_hops_cn004238_na_en_587a5d300b.pdf"><strong>check out our application note today</strong></a>.</p><h4><strong>FAQs</strong></h4><div><div><h5><strong>What are the key compounds in hops analyzed by HPLC?</strong></h5><p>Bitter acids, namely α-acids and β-acids, especially the six major bitter acids (humulones and lupulones) that define hop bitterness and are required for quality control and brewing decisions.</p></div><div><h5><strong>What column types are recommended for hop acid separations?</strong></h5><p>Classical C18 Silica columns are ideal for bitter acids separation. UHPLC compatible columns with particle size ≤ 2 µm boost analysis with higher flow rate, thus shorter analysis time and solvent consumption.</p></div><div><h5><strong>What’s the best workflow for hops analysis?</strong></h5><p>A typical workflow for hop analysis consists of three main steps: extraction of the target compounds, clean-up of co-extracted matrix components, and instrumental analysis by HPLC or UHPLC. To keep this workflow efficient and reproducible, you should minimize manual handling and add in automation when possible. Integrating online SPE into your LC instrument allows sample clean-up to run automatically, reducing hands-on time, solvent use, and variability while delivering faster, more reliable results.</p></div></div><blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a>&nbsp;#UHPLC #Automation</p></blockquote></div></div>

How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors

<p><strong>In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</strong></h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul><p>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.</p><h5><strong>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</strong></h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul><p>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.</p><h5><strong>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</strong></h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul><p>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.</p><h5><strong>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</strong></h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul><p>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.</p><h5><strong>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</strong></h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul><p>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.</p><h5><strong>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</strong></h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul><p>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.</p>

Webinars LabRulezLCMS Week 27/2026

<p><strong>The international standard ISO/IEC 17025 [1] clause 7.7.2 with regards to ensuring the validity of results, requires a laboratory to participate in proficiency testing (PT) and/or to participate in interlaboratory comparisons (ILCs) other than PT. However, no guidance as to what these other ILCs might be, or indeed how they might compare in relation to PT, is provided. This leaflet aims to outline other ILCs available, pointing out some of the limitations as compared to PT.&nbsp;</strong></p><p>There are three major types of ILCs in which a group of laboratories analyses identical portions from a homogeneous, stable test sample, with each type being characterized by its intended purpose [2,3]:</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg 245w," sizes="100vw" width="1507" height="607"></p><p>A key point is that PT is an ILC that is designed to specifically evaluate the performance of participants, providing them with an infrastructure to monitor and assess the validity of their results. This is not the key purpose of the two other ILCs, and thus depending on their intended purpose they have a number of limitations that laboratories need to be aware of when using them rather than PT. Only proficiency testing should have been conducted in full compliance to the competency standard ISO/IEC 17043 [4].&nbsp;</p><h4><strong>Evaluation of measurement procedure performance&nbsp;</strong></h4><p>These ILCs, are designed to assess how a particular measurement procedure performs and whether it is fit for its intended purpose. These ILCs are often referred to as a ‘method performance study’ or a ‘collaborative study’ [2]. Some limitations of using such ILCs for monitoring laboratory performance as an alternative to PT are:&nbsp;</p><ul><li>All participants use the same measurement procedure i.e. the measurement procedure that is being validated. Unless the measurement procedure is being routinely used by the participating laboratory such an ILC will not assess the routine performance of the laboratory using their own routine measurement procedure. Nor does it enable them to compare their performance with other laboratories using alternative measurement procedures.&nbsp;</li><li>The measurement procedure is likely to be new, so participants may not have established stable procedures and quality control, so performance may not reflect routine use.&nbsp;</li><li>The report from such an ILC would not provide information on the performance of the participant, if the statistical model used makes the assumption that all laboratories perform with equal variability.</li><li>The design of the study may require the participants to assess multiple samples and/or to undertake multiple replicate analysis on the test materials. This may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li><li>The conclusion of the ILC might be that the measurement procedure being assessed is not valid.&nbsp;</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg 157w," sizes="100vw" width="570" height="566"></p><h4><strong>Characterisation of candidate reference materials&nbsp;</strong></h4><p>These are ILCs that assign a property value to a candidate reference material, usually with a stated measurement uncertainty. These are often referred to as a ‘material certification study’ [2]. Limitations of using such ILCs as an alternative to PT are:&nbsp;</p><ul><li>The analysis of the candidate reference material is likely to require additional care in exercising the routine measurement procedure, such as substantially more replication, and/or may require quite different reporting formats (e.g. all valid replicates instead of a mean result). Thus, such an ILC will not necessarily provide an evaluation of the performance of the laboratory based on the routine use of their measurement procedures.&nbsp;</li><li>The report from such an ILC may include the results from the participating laboratories but would not provide information on the performance of each laboratory.&nbsp;</li><li>Participating laboratories may be required to assess multiple samples and/or to undertake multiple replicate analysis on the candidate reference materials. Thus this may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li></ul><h4><strong>Other ILCs&nbsp;</strong></h4><p>Some other ILCs, whilst not being conducted in full compliance of the requirements of ISO/IEC 17043, may be designed to evaluate participant performance. These would include small ILCs as defined by EA‑2/21 [5] or split sample ILCs i.e. where a portion of a single sample is sent to two or three laboratories. Whilst these ILCs are specifically designed to evaluate participant performance, they may not provide a comprehensive comparison that a PT might provide. In such cases the laboratory needs to evaluate carefully the competence of the organization managing the ILC and the suitability of the service externally provided (ISO/IEC 17025 clause 6.6).</p>

Interlaboratory comparisons other than proficiency testing

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<p><strong>This webinar hosted by Select Science together with Metrohm shows the possibilities of ion chromatography systems that are optimized for multiple applications.</strong></p>


Metrohm: Ion chromatography for multiple applications

Ion chromatography for multiple applications

Possibilities of ion chromatography systems that are optimized for multiple applications.

<p><strong>Learn in this webinar how automatic calibration in ion chromatography can help you save time and gain reliability.</strong></p>


Metrohm: Automatic calibration in ion chromatography

Automatic calibration in ion chromatography

Learn in this webinar how automatic calibration in ion chromatography can help you save time and gain reliability.

<p><strong>Learn how you can determine glyphosate and its primary metabolite AMPA in drinking water by ion chromatography in a straightforward manner.</strong></p>


Metrohm: Glyphosate and AMPA analysis by ion chromatography

Glyphosate and AMPA analysis by ion chromatography

Learn how you can determine glyphosate and its primary metabolite AMPA in drinking water by ion chromatography in a straightforward manner.

<p>The future begins with innovation. For 45 years, innovative Thermo Scientific Dionex Ion Chromatography (IC) systems have enabled extraordinary breakthroughs and discoveries. Laboratory professionals like you have been propelling science forward with reliable, reproducible analyses based on Dionex solutions. Confidence drives your passion for excellence and exploration every day.</p>
<p>Your scientific talent, combined with our exceptional IC innovations, continues to deliver impact for the world’s scientific community, empowering health and safety in society and spearheading positive environmental developments.</p>
<p>Together, let us advance IC science for greater future impact. Because we can’t meet in person, we’re meeting virtually – with events happening over three days to conveniently engage all global regions and time zones. One day for Asia, one for Europe and one for the Americas (although you’re welcome to join the symposium any day to post questions and view sessions of interest).</p>
<h3><strong>Attend the virtual event to:</strong></h3>
<ul>
<li>Hear from industry experts about the impact of ion chromatography on applied sciences</li>
<li>Learn how IC is helping your colleagues achieve new advancements</li>
<li>Raise topics pertaining to ion chromatography and its application, which will be addressed by a panel of IC experts in the forum</li>
<li>Browse resources to see how IC can be applied in your lab</li>
</ul>
<h3><strong>27th October  (Tuesday) Agenda - Ideal for the Americas</strong></h3>
<ul>
<li><p><strong>8:00 am PT (17:00 CEST): Plenary Talk: 45 Years of Ion Chromatography – Milestones of the Development</strong> (Dr. Joachim Weiss | Technical Director | Thermo Fisher Scientific)</p>
</li>
<li><p><strong>8:45 am PT (17:45 CEST): What I See Undersea with IC</strong> (Nathan F. Dalleska, Ph.D. | Director , Environmental Analysis Center | Caltech)</p>
</li>
<li><p><strong>9:05 am PT (18:05 CEST): Improved understanding of the hydrogeochemistry of arsenic and selenium in ambient and industrial waters</strong> (Dirk Wallschläger | Professor, Environmental Chemistry |Trent University)</p>
</li>
<li><p><strong>9:25 am PT (18:25 CEST): Fundamentals of IC and Virtual Column</strong> (Dr. Charles Lucy | Professor Emeritus | University of Alberta)</p>
</li>
<li><p><strong>Q&amp;A Session</strong></p>
</li>
<li><p><strong>9:45 am PT (18:45 CEST): Forum</strong> (Facilitator: Dana Stradtner)</p>
</li>
<li><p><strong>Panelists: Dr. Jeffrey Rohrer</strong> (Thermo Fisher Scientific</p>
</li>
<li><p><strong>Panelists: Kirk Chassinol</strong> (Thermo Fisher Scientific)</p>
</li>
<li><p><strong>Panelists: Michael Hvizd</strong> (Thermo Fisher Scientific)</p>
</li>
<li><p><strong>10:30 am PT (19:30 CEST): End of Day 3</strong></p>
</li>
</ul>


Thermo Fisher Scientific: Global IC Virtual Symposium - Day 3

Global IC Virtual Symposium - Day 3

<p><strong>For food and beverage analysis, HPLC is a known and established analysis technique, whereas ion chromatography is typically known for ion analysis in water samples.</strong></p>
<p>But is this classification correct? Which analyses can be done with ion chromatography for food and beverage samples? What are the benefits of working with Metrohm IC? And what other Metrohm instrumentation is available for food and beverage analysis?</p>
<p>This webinar will answer these questions and provide insights into how to optimize your IC analysis. </p>
<h3><strong>Who should attend the webinar:</strong></h3>
<p>The webinar provides valuable input for chemists as well as for lab technicians, lab staff or students who handle food and beverage analysis. It provides practical tips for correct and time-saving analysis.</p>
<h3><strong>By attending this webinar you will:</strong></h3>
<ul>
<li>Get a better understanding of the differences between the two techniques, IC and HPLC</li>
<li>Learn how you can save time in daily routine work using Metrohm Inline Sample Preparation Techniques</li>
<li>Get an understanding of the applications that can be done with IC or other Metrohm instrumentation for food and beverage analysis</li>
</ul>
<blockquote>
<p><strong>Presenter: Dr. Gabriele Zierfels (Sr. Product Specialist IC, Metrohm International Headquarters)</strong></p>
</blockquote>
<blockquote>
<p>Dr. Gabriele Zierfels studied food chemistry and developed HPLC methods for the determination of antibiotics in animal food matrices for her PhD. As a laboratory manager, she worked for almost 14 years in the field of chemical analysis for textile and leather testing in a contract lab. During this time, she gained extensive practical knowledge in sample preparation techniques and the operation of HPLC, GC-MS, and LC-MS. Gabriele has worked in the Competence Center Ion Chromatography at Metrohm for more than 10 years. As a Sr. Product Specialist, she is responsible for method development – especially for the food industry – as well as customer support and training.</p>
</blockquote>


Pixabay/Peggy cci: Solutions for Analysis in Food and Beverages Save Time by using IC

Solutions for Analysis in Food and Beverages Save Time by using IC

<p><strong>Reaching further in your metabolomics study with IC, GC and LC-MS analysis.</strong></p>
<p>The comprehensive analysis of small endogenous and exogenous molecules in a biological system results in a better understanding of metabolomic processes. The characterization of metabolic phenotypes enables biomarker discovery, disease taxonomy, and tracking treatment effects. </p>
<p>The metabolomic challenge includes a large number of compounds with diverse physicochemical properties. This leads to new analytical challenges when a more comprehensive coverage of the metabolic landscape is needed.</p>
<p>High-resolution accurate mass measurement (HRAM) mass spectrometry (MS) is an essential tool in metabolome analysis. Thermo Scientific Orbitrap-based mass spectrometers provide a leading combination of sensitivity, mass accuracy, and resolving power that helps answer the most challenging analytical tasks.</p>
<p>MS, however, suffers from disadvantages such as ion suppression and the differentiation of isomers. The combination of MS with separation techniques addresses such issues. Different chromatographic approaches can be used and fine-tuned to a specific set of metabolites, including polar and ionic compounds, glycans, volatile compounds, and steroids. The comprehensive analysis of the metabolome, however, requires a combination of this method.</p>
<h3><strong>We will present three topics:</strong></h3>
<ul>
<li>The untargeted screening workflow for identification and comparison of different wine origin using the GC Orbitrap technology and Compound discoverer,</li>
<li>The combination of Ion Chromatography (IC) with HRAM to determine polar and ionic components, </li>
<li>A HPLC-Orbitrap example study to showing the changes observed in mice plasma composition depending on the diet type.</li>
</ul>
<p>All experimental data were processed with the Thermo Scientific Compound Discoverer software, to comprehensively profile the samples, detect more compounds, and identify unknowns. </p>
<p>We will introduce our newest member of the LC-MS family, the Thermo Scientific Orbitrap Exploris mass spectrometer.</p>
<h3><strong>What you will learn:</strong></h3>
<ul>
<li>MS instrumentation designed for exceptional usability resulting in increased productivity in metabolomics</li>
<li>Benefits of high-resolution accurate mass spectrometry (HRAM) in unknown analysis and research</li>
<li>Analytical solutions for sample profiling</li>
<li>Examples of complete IC-MS, GC-MS, and LC-MS workflows for unknown identification using Orbitrap technology and Compound Discoverer software</li>
</ul>
<blockquote>
<p><strong>Presenter: Anas Kamleh (Expert Sales Support, Metabolomics, Thermo Fisher Scientific)</strong></p>
</blockquote>
<blockquote>
<p>Anas joined Thermo Fisher Scientific in 2015 as an application specialist in metabolomics. He is driving a new role since 2018 that entitles supervising collaboration projects and ensuring our hardware and software products provide the best experience for Orbitrap metabolomics users. His industrial and academic background extend to close to 15 years of experience in the field of metabolomics spanning a PhD thesis from University of Strathclyde, Scotland and postdoctoral research positions at Imperial College London and Karolinska Institutet in Stockholm, Sweden.</p>
</blockquote>
<blockquote>
<p><strong>Presenter: Racha Mailler (Sales Support Specialist GCMS/MS, EFS-Industrial, Thermo Fisher Scientific)</strong></p>
</blockquote>
<blockquote>
<p>Racha joined Thermo Fisher Scientific in 2016 as Technical support specialist for GC and GC/MS. She is driving a new role since 2018 as an application specialist for GC, GCMS and Orbitrap technologies. Her role has expand to cover EMEA zone. Her everyday job is to help our GCMS users getting the best out of their instrument by training them on the software and advising them with the method development. Racha holds a PhD degree in Analytical chemistry from the university of Lille 1.</p>
</blockquote>
<blockquote>
<p><strong>Presenter: Detlef Jensen (Sr. Expert, SST, EFS-Industrial, Thermo Fisher Scientific)</strong></p>
</blockquote>
<blockquote>
<p>Detlef holds a Ph.D. in Chemistry from the Technical University Berlin/Germany. For more than 30 years, he covered many positions at Dionex, now Thermo Fisher Scientific in ion chromatography. Since March 2013, he has been supporting our users and field teams all over Europe in his role as European Ion Chromatography Expert.</p>
</blockquote>


Thermo Scientific: Omics Winter Webinars - Metabolomics session 4: Chromatographic solutions

Metabolomics session 4: Chromatographic solutions

<p><strong>Haloacetic acids (HAAs) are disinfection byproducts caused by the reaction of chlorine and naturally occurring dissolved organic matter present in drinking water.  Five commonly found HAAs are regulated by the EPA with a Maximum Contaminant Level (MCL) of 60 ug/L.</strong></p>
<blockquote>
<p><strong>Presenter: Emily Parry, PhD (LC/MS Applications Scientist, Agilent Technologies, Inc.)</strong></p>
</blockquote>
<blockquote>
<p>Emily Parry received her PhD degree in Environmental Chemistry from the University of California, Davis. She joined Agilent after completing her postdoctoral work at the Environmental Protection Agency (EPA). Her specialty is method development and measurement of emerging contaminants.</p>
</blockquote>


Agilent Technologies: Haloacetic Acids Analysis by IC-TQ

Haloacetic Acids Analysis by IC-TQ

<p><strong>Over the last few years, attention to food quality and ingredients has seen a massive increase. Public authorities have responded to this demand with more stringent quality standards and labelling requirements (for example, EU regulation 1169/2011 and the US regulation 21CFR101). Hence, fast, robust and reliable analytical techniques have become ever more necessary.</strong></p>
<p>This New Food webinar in association with Metrohm will begin with an overview of the latest analytical techniques used for beverage analyses and the challenges they present. The presenters will then take a closer look at the benefits of ion chromatography for beverage analysis.</p>
<p>The main part of the webinar will guide attendees through the numerous dedicated Metrohm IC applications for beverage analysis. The audience will learn about the versatility of ion chromatography for food testing and how it can help increase the efficiency of their daily routine analytics.</p>
<h3><strong>Key learning objectives:</strong></h3>
<ul>
<li>Understand the advantages of using ion chromatography over competing methods for beverage analysis</li>
<li>Learn how automated inline sample preparation techniques can address the challenge of difficult samples, so they can be analysed completely unattended</li>
<li>Establish how ion chromatography works for multi-parameter analysis of beverages</li>
<li>Learn about the many possible applications of ion chromatography within beverage analysis.</li>
</ul>
<blockquote>
<p><strong>Presenter: Dr Gabriele Zierfels (Senior Product Specialist, Metrohm International)</strong></p>
</blockquote>
<blockquote>
<p>Dr Gabriele Zierfels studied food chemistry and for her PhD developed HPLC methods for the determination of antibiotics in animal food matrices. She worked for almost 14 years as a laboratory manager in the field of textile and leather testing at a leading contract laboratory. During this time, she gained extensive practical expertise in sample preparation techniques and the operation of a diversity of analytical methods, e. g., HPLC, GC-MS, and LC-MS.</p>
</blockquote>
<blockquote>
<p>For more than 10 years, Gabriele has been working at the Competence Center Ion Chromatography at Metrohm International HQ in Switzerland. As a Senior Product Specialist, she is responsible for product and method development, especially for the food industry, as well as for customer support and training.</p>
</blockquote>


Pixabay/bridgesward: Improving your analytics for beverage analysis with ion chromatography

Improving your analytics for beverage analysis with ion chromatography

<p><strong>There are many challenges in carbohydrate analysis, whether we want a simple profile or to discover the complexity of glycans. To separate all the groups of glycans into their size and charge can be very complicated and challenging, especially with one method.</strong></p>
<p>For many years Thermo Scientific Dionex CarboPac PA100 and PA200 Columns have provided an abundance of information in glycan identification analysis with High-Performance Anion Exchange-Pulsed Amperometry Detection (HPAE-PAD) standalone and with Mass Spectrometry. Here we will find out about the Thermo Scientific Dionex CarboPac PA300 column and what areas we can resolve to understand glycans better, whether it&#39;s O-linked or N-linked, and if it&#39;s charged or neutral.</p>
<h3><strong>Learning points</strong></h3>
<ul>
<li>Latest advances in carbohydrate column technology</li>
<li>Capabilities of the Dionex CarboPac PA300 column</li>
<li>Increase efficiency and cost savings</li>
</ul>
<h3><strong>Who should attend?</strong></h3>
<ul>
<li>Laboratory analysts or researchers performing glycan analysis</li>
<li>Laboratory managers looking to progress and simplify their glycan analysis</li>
<li>Biopharma analysts looking at complex carbohydrates</li>
</ul>
<blockquote>
<p><strong>Presenter: Wai-Chi Man (Product Marketing Manager, Thermo Fisher Scientific)</strong></p>
</blockquote>
<blockquote>
<p>Wai-Chi has over 34 years in Analytical Chemistry experience in a wide range of techniques (IC, LC, GC, CE, MS, NMR, ICP, FTIR, etc), and has specialized in Ion Chromatography for over 32years. She spent 7 years at Wellcome Research supporting Structural Elucidation for Medicinal Chemists with a total of 20 years in the Pharma industry as part of GlaxoSmith Kline, from research, to transferring methods, to manufacturing. She has worked on and has been responsible for many drugs, from diabetes to anti-virals.</p>
</blockquote>
<blockquote>
<p><strong>Moderator: Gemma Ellison (EMEA ICSP Regional Marketing Manager, Thermo Fisher Scientific)</strong></p>
</blockquote>
<blockquote>
<p>Gemma Ellison is the EMEA marketing manager for ion chromatography, sample preparation and discrete analyzer products. As a former analytical chemist, Gemma’s career began in the pharmaceutical industry building expertise in chromatographic and analytical techniques before joining Thermo Fisher Scientific as an ion chromatography and sample preparation specialist for the UK and Ireland. As a specialist Gemma supported a wide variety of application areas and challenges faced by customers giving her a solid understanding of the solutions required. Gemma holds a Bachelor of Science degree in Applied Chemistry from Sheffield Hallam University.</p>
</blockquote>


Thermo Fisher Scientific: Advancing carbohydrate analysis

Advancing carbohydrate analysis - Glycans

<p><strong>Carbohydrate analysis is challenging. Usually, sialic acids, simple mono, and disaccharides to the more complex oligosaccharides require multiple method conditions to separate. The eluents can range from simple hydroxide based eluents to a combination of hydroxide and acetate buffers.</strong></p>
<p>Sourcing the purest chemicals to use for the eluent can be challenging when the production process changes. When manually preparing the eluent, we have to take many cautious steps to ensure good reproducible data, which adds another level of complexity to method preparation. </p>
<p>Automated eluent generation can simplify carbohydrate analysis by providing cleaner and purer eluents, which in turn resolves challenges in reproducibility, and minimizes maintenance to your instrument. It can save time and resources so we can work smarter and be more productive.</p>
<h3><strong>Learning points:</strong></h3>
<ul>
<li>Capabilities of eluent and dual eluent generation</li>
<li>Latest advances in carbohydrate technology</li>
<li>How to increase productivity and cost savings.</li>
</ul>
<h3><strong>Who should attend:</strong></h3>
<ul>
<li>Laboratory analysts or researchers performing carbohydrate analysis</li>
<li>Analysts analyzing food and beverages for sugar content</li>
<li>Biopharma analysts looking at simple to complex carbohydrates</li>
</ul>
<blockquote>
<p><strong>Presenter: Wai-Chi Man (Product Marketing Manager, Thermo Fisher Scientific)</strong></p>
</blockquote>
<blockquote>
<p>Wai-Chi has over 34 years in Analytical Chemistry experience in a wide range of techniques (IC, LC, GC, CE, MS, NMR, ICP, FTIR, etc), and has specialized in Ion Chromatography for over 32years. She spent 7 years at Wellcome Research supporting Structural Elucidation for Medicinal Chemists with a total of 20 years in the Pharma industry as part of GlaxoSmith Kline, from research, to transferring methods, to manufacturing. She has worked on and has been responsible for many drugs, from diabetes to anti-virals.</p>
</blockquote>
<blockquote>
<p><strong>Moderator: Gemma Ellison (EMEA ICSP Regional Marketing Manager, Thermo Fisher Scientific)</strong></p>
</blockquote>
<blockquote>
<p>Gemma Ellison is the EMEA marketing manager for ion chromatography, sample preparation and discrete analyzer products. As a former analytical chemist, Gemma’s career began in the pharmaceutical industry building expertise in chromatographic and analytical techniques before joining Thermo Fisher Scientific as an ion chromatography and sample preparation specialist for the UK and Ireland. As a specialist Gemma supported a wide variety of application areas and challenges faced by customers giving her a solid understanding of the solutions required. Gemma holds a Bachelor of Science degree in Applied Chemistry from Sheffield Hallam University.</p>
</blockquote>


LCMS webinars focusing on IC - page 12