Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

News from LabRulezLCMS Library - Week 20, 2026

🎤Lindsay Repka&nbsp;Lindsay Repka is a chemistry professor at Middlebury College whose research explores photoredox chemistry, catalyst stability, and visible-light-driven transformations. Her lab emphasizes both mechanistic insight and hands-on student training in advanced analytical instrumentation.In this episode of Concentrating on Chromatography, we sit down with Lindsay Repka to discuss how LC-MS and GC-MS transformed her lab’s approach to photoredox chemistry.What began as a project to develop a visible-light photocrosslinking handle unexpectedly led to a major discovery: the solvent (DMF) was reacting with the photocatalyst itself. Using high-resolution LC-MS, Lindsay’s team observed multiple solvent adducts forming — sometimes with complete catalyst consumption. That discovery reshaped their research direction.This episode is a rare deep dive into both LC-MS and GC-MS within the same research project, showing how chromatography-driven insight can turn unexpected degradation into productive new reactivity.If you work in:<ul><li>Photoredox chemistry</li><li>Reaction optimization</li><li>Mass spectrometry method development</li><li>Catalyst screening</li><li>Academic synthetic chemistry</li></ul>…this conversation will resonate.<h4>Key Topics Covered</h4><ul><li>Photocatalyst stability in DMF, DCE, and MeCN</li><li>Demethylation under mild visible-light conditions</li><li>High-resolution Q-TOF LC-MS quantitation</li><li>Internal standard methodology in GC-MS</li><li>Signal-to-noise improvement using extracted ion chromatograms</li><li>Reaction reproducibility and quality control strategy</li></ul><h3>Video Transcription</h3>In this interview, Lindsay Repka discusses the role of photoredox chemistry in method development, photocrosslinking, solvent activation, catalyst stability, and the use of LC-MS and GC-MS to understand reaction pathways.<h4>What is photoredox chemistry?</h4>Photoredox chemistry uses light to activate a photocatalyst, which can either donate or accept an electron from a substrate. This generates radical species that can undergo new types of reactivity.Repka explains that one of the key advantages of photoredox chemistry is its ability to operate under mild conditions, often using visible light. This makes it attractive for sensitive molecules and biological systems, including potential applications in drug release, live-cell chemistry, and drug development.<h4>Photocrosslinking and drug-target interactions</h4>Repka’s group initially aimed to develop visible-light photocrosslinking methods to study drug interactions with biological targets. Photocrosslinking can covalently link a drug to its target, making it easier to identify the biological target of a compound.Traditional photocrosslinking often uses UV light, but visible light offers milder and more biologically compatible conditions.<h4>From photocrosslinking to solvent activation</h4>The project shifted direction when the desired photocrosslinking reactions gave very low yields. Instead of reacting with amino acids as intended, the radical intermediate mainly reacted with itself.The team explored different solvents to stabilize the radical, but found that some solvents, such as DMF, were not inert. Instead, the solvent reacted with the photocatalyst or radical species, revealing unexpected solvent activation chemistry.<h4>Why LC-MS was critical</h4>LC-MS became essential because the reaction mixtures were too complex and low-yielding for NMR or GC-MS. High-resolution LC-MS allowed the team to detect minor products, identify photocatalyst modifications, and observe solvent addition products with high confidence.Repka emphasizes that LC-MS was both a qualitative and quantitative tool. It was used quantitatively to measure how much photocatalyst remained after light exposure, and qualitatively to identify degradation products and new reactivity pathways.<h4>Photocatalyst stability workflow</h4>The team developed a systematic workflow to evaluate photocatalyst stability under light exposure. Because catalyst degradation was time-sensitive, reproducibility required strict control of experimental conditions.Key steps included using a glove box, commercial deoxygenated solvents, accurate weighing on a microbalance, controlled light exposure in a photoreactor, and temperature control with a chiller. These precautions helped reduce day-to-day variability and enabled reproducible measurements of catalyst remaining.<h4>Quantifying catalyst remaining by LC-MS</h4>Calibration curves were prepared using pure photocatalyst solutions and LC-MS ion areas. The team worked in the nanomolar range to maintain linearity.Instead of relying only on total ion chromatograms, they used extracted ion chromatograms (EICs) to improve signal-to-noise for the photocatalyst signal. Baseline subtraction was also applied to reduce noise and improve quantitation at low concentrations.Calibration curves and quality control samples were prepared and measured on the same day as the reaction samples, because LC-MS signal intensity can vary significantly from day to day.<h4>Why GC-MS was used later</h4>For demethylation studies, the group moved to GC-MS because the substrates and products were small and volatile enough for gas chromatography. GC-MS was better suited for rapidly screening many reaction conditions, including different photocatalysts, solvents, and oxidants.Instead of generating full calibration curves for every experiment, the team used an internal standard to streamline analysis and determine percent conversion based on relative response factors.<h4>Practical lessons from the project</h4>Repka highlights the importance of careful sample preparation, especially when working with very small quantities of photocatalysts. A high-quality microbalance, stable granite platform, antistatic tools, and aluminum weigh boats improved weighing accuracy and reproducibility.She also argues that mass spectrometry should be a standard part of photoredox method development because it can reveal minor products, support reaction optimization, and provide insights that may be difficult or impossible to obtain by NMR alone.<h4>Final perspective</h4>Beyond the chemistry, Repka emphasizes the importance of student training and mentoring. For her, research is not only about discovering new reactivity but also about giving students practical experience, helping them develop independence, and preparing them for future scientific careers.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Using LC-MS & GC-MS to Decode Photoredox Catalyst Stability

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The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

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Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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<h4>Overview</h4><ul><li>Overview of HPLC troubleshooting approach</li><li>Main HPLC components and potential issues</li><li>General Chromatographic Problems</li></ul><h4>Key Learning Points</h4><ul><li>Understand systematic elimination as the best approach for troubleshooting</li><li>Learn the potential issues from different HPLC parts</li><li>Discover chromatographic problems, including abnormal pressure, various retention time, data quality and baseline issue.</li></ul><h4>Who Should Attend</h4><ul><li>Any scientists who would like to gain more knowledge on HPLC troubleshooting or anyone who is experiencing challenges on system or column related issues.</li></ul><blockquote>Speaker: Grace Guo (Senior Technical Specialist, Phenomenex)Grace Guo graduated from the Johns Hopkins University with a Master’s degree in Material Science and Engineering. She joined Phenomenex Technical Team in 2017 providing technical support globally. With more than 9 years of experience on SPE, HPLC and GC, she delivered 80+ seminars and webinars globally covering food and environmental, clinical, pharma and biopharma industry.</blockquote>

Phenomenex: LC Troubleshooting: Crack the Code with Systematic Solutions!

LC Troubleshooting: Crack the Code with Systematic Solutions!

Discover chromatographic problems, including abnormal pressure, various retention time, data quality and baseline issue.

LC Troubleshooting: Crack the Code with Systematic Solutions

<div><div>Let's go back to the Basics of Chromatography, Mass Spectrometry, Data Analysis and Trouble-shooting: understanding the importance of the basics will enable you to be more effective in your daily laboratory life.This journey started in 2024 and we are now pleased to invite you to join us to go deeper in fundamentals, troubleshooting and application specific topics.<h3>EPISODE 2: A Systematic Way to Approach your LC Troubleshooting Journey</h3>What will you learn?In this session, we will cover some of the most common LC troubleshooting issues and how to address them:<ul><li>Poor Peak Shapes</li><li>Varying Reproducibility</li><li>Contamination and Carry Over</li><li>Over Pressure</li></ul>What's next?<ul><li>Check out the full Webinar Series Episodes List <a target="_blank" rel="noopener noreferrer" href="https://event.on24.com/eventRegistration/EventLobbyServlet?target=reg20.jsp&amp;eventid=4817889&amp;sessionid=1&amp;key=B9182AE813B3F8A30217606156F96B75&amp;groupId=5901784&amp;sourcepage=register">here</a></li></ul>Not sure to be able to attend live sessions?&nbsp;<ul><li>Register today and watch the webinars on-demand</li></ul><blockquote>Presenter: Nicole Baumgarten (Senior Sales Application Chemist, Waters)Nicole is part of the Customer Success team at Waters with a focus on LC-MS/MS application and sales support. She works in this role for more than 10 years and gained experience in previous roles as analyst for biopharmaceuticals in process controls at Boehringer Ingelheim and research Assistent at Zentrum für Humangenetik und Laboratoriumsmedizin in Munich after finishing a Master of Chemical engineering at Hochschule Fresenius, Idstein.</blockquote></div></div>

Waters Corporation: Back to Basics Advanced Webinar Series 2025

Back to Basics Advanced - Troubleshooting: LC

In this session, we will cover some of the most common LC troubleshooting issues .

<div><div><h3>Oligos, Oligos, Oligos!</h3>Oligonucleotide therapeutics and reagents are everywhere. They are helping the world address previously untreatable diseases, and they are also being used in new ways to sensitively quantify and sequence important translational research samples. Whether it be a CRISPR guide RNA, lipid conjugated anti-sense oligonucleotide, or a specially modified assay probe, these molecules must be tested to ensure they have the right sequence, pinpoint-specific modifications, conjugate moieties and purities. &nbsp;And when dosed as a therapeutic, they need to be carefully quantified to confirm pharmacokinetic models. &nbsp;Join the Waters Chromatography and LC-MS Team and their collaborators from MRIGlobal, Regeneron, and Acuitas Therapeutics in this special episodic, 3 week webinar series to learn more about the science of oligos and the power of LC-based approaches to analyze them.&nbsp;<h3>EPISODE 3</h3><h4>Part 1: Deformulation and Nucleic Acid Quantification in LNPs Using Size-Exclusion Chromatography</h4>Learn about an alternative high-throughput approach for the deformulation and quantification of nucleic acid payloads in lipid nanoparticles (LNPs). Using a low adsorption 2.5 µm SEC 450 Å Column, this size-exclusion chromatography (SEC) method employs deformulating conditions with detergent and organic solvents in the mobile phase to disrupt LNPs without prior sample treatment.<blockquote>Presenter: Mateusz Imiolek, PhD (Waters Corporation, Geneva, Switzerland)Mateusz joined Waters in 2022 where he has been studying challenges associated with characterizing gene therapeutics and ensuring that new Waters products are fit for this purpose. Before that, he completed his academic training in bioorganic chemistry, which allows him to apply detailed molecular insights to ensure the newly developed columns and assays are carefully designed for such analytes.</blockquote><h4>Part 2: High-Throughput Nucleic Acid and Lipid Adduct Quantification in LNPs Using Ion-Paired Reverse-Phase Chromatography</h4>A high-throughput LC-based assay developed for nucleic acid quantification in lipid nanoparticles (LNPs). By using a short, low adsorption 2.5 µm BEH C18 column, the assay allows for the efficient separation of LNP payloads, including guide RNA and mRNA, from detergent-disrupted samples. The assay is designed for excellent robustness and reproducibility, making it suitable for routine application.<blockquote>Presenter: Razvan Cojocaru (Research Scientist II, Acuitas Therapeutics)Razvan has been a research scientist in the Analytical department at Acuitas Therapeutics since 2021, where he focuses on developing new methodology for characterizing nucleic acids in LNP therapeutics. Prior to Acuitas, Razvan completed his studies at Simon Fraser University in Molecular Biology and Biochemistry, where he focused on Ribozyme and RNA Aptamer in vitro selection and optimization.</blockquote></div></div>

LC for LNP RNA Molecular Composition Analysis

Join Waters Chromatography, LC-MS Team & collaborators for a 3-week webinar series on oligos science & LC-based analysis.

Polysaccharide vaccines play a critical role in preventing diseases caused by encapsulated bacteria, such as Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis. These vaccines are in complex polysaccharide protein conjugate molecules that stimulate the immune system and provide long-lasting protection.&nbsp;In this webinar, we will explore analytical techniques used to evaluate the efficacy, safety, and quality of polysaccharide vaccines.Key learning objectives:<ul><li>Understand regulatory perspectives and the resources provided by USP for the characterization of polysaccharide vaccines</li><li>Explore the benefits of LC coupled with Multi-Angle Light Scattering (MALS) as a quality attribute</li></ul>Who should attend:<ul><li>Analytical scientists involved with the characterization of polysaccharide vaccines.</li></ul><blockquote>Presenter: Mandy Alger (Senior Scientist II, Global Biologics US Pharmacopeia)Mandy has been working on biopharmaceuticals and vaccine development for more than twenty years. Prior to her tenure at the United States Pharmacopeia, she was employed by organizations like GlaxoSmithKline (GSK), the National Institutes of Health (NIH), and AstraZeneca (AZ).Joined the Global Biologics Division of USP in 2021 and currently serves as a Senior Scientist II at USP. Collaborates with the USP teams, the pharmaceutical industry, and the FDA to develop USP reference standards and documentation standards pertaining to pharmaceuticals, includingcomplex Proteins, Heparin, Dextran, Oxytocin, Oligosaccharide, and polysaccharide vaccine molecular weight standards.Presenter:&nbsp;Katayoun Morakabi, Ph.D. (Application Scientist Waters l Wyatt Technology)Katayoun Morakabi is an application scientist in the Analytical Sciences team at Waters | Wyatt Technology. She assists customers in identifying the best light scattering solutions for their analytical needs by leveraging her expertise in multi-angle light scattering. She performs feasibility studies to characterize customer samples and participates in Wyatt’s Light Scattering University. She completed her Ph.D. in Biochemistry from the University of Texas at Dallas. Her doctoral work focused on characterizing the structure, dynamics, and functional outcomes of histone acetyltransferases and histone chaperone proteins.Moderator: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.</blockquote>

Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

Polysaccharide Vaccines

In this webinar, we will explore analytical techniques used to evaluate the efficacy, safety, and quality of polysaccharide vaccines.

Oligonucleotides are highly important biopharmaceuticals with strongly increasing relevance. Due to the high similarity of impurities originating from the synthesis, however, purification can be challenging.This seminar demonstrates a successful large scale purification of an oligonucleotide using anion exchange chromatography (AEX) as well as efficient quality control by applying ion-pairing reversed phase chromatography (IP-RP).<h4>Content</h4><ul><li>How to perform a large scale AEX purification: Design of preparative system and useful features</li><li>Benefits and limitations using AEX for preparative purification</li><li>What to consider for developing an IP-RP methods: selection of stationary phase, ion-pair reagent, and solvent conditions</li><li>How to improve analytical results by utilizing bioinert column hardware</li></ul><h4>Who are the seminar for?</h4><ul><li>Advanced and beginners interested in peptides and/or oligonucleotides.</li></ul><blockquote>Presenter: Dr. Ulrike Krop (KNAUER Wissenschaftliche Geräte GmbH)Presenter: Dr. Daniel Eßer (YMC Europe GmbH)</blockquote>

KNAUER: Purification and quality control of oligonucleotides

Purification and quality control of oligonucleotides

This seminar demonstrates a successful large scale purification of an oligonucleotide using anion exchange chromatography (AEX).

Ensuring food safety and quality is increasingly challenging. Whether you're analyzing for pesticide residues, ensuring that label claims are correct and accurate, or profiling for food authenticity, Agilent works with you, helping you deliver what your customers demand.Agilent's food and beverage testing products and services keep you at the forefront of the latest trends, ahead of issues, and focused where you need to be – delivering highest quality and value to your customers.In this webinar you will learn:<ul><li>Latest trends and regulatory updates in food testing.</li><li>Agilent latest HPLC &amp; UHPLC series introduction.</li><li>Agilent application solutions for various important food quality parameters for food &amp; beverages testing.</li></ul>Who should attend?<ul><li>Managers &amp; Scientists from food testing labs, food processing companies, contract testing labs, government regulatory bodies, labs, etc.</li></ul><blockquote>Presenter: Dr. Lalitha R Gowda (Chief Scientist (Rtd), CSIR - Central Food Technological Research Institute)Dr. Gowda retired as Chief Scientist at CSIR-Central Food Technological Research Institute, Mysore in the year 2014. She obtained her Ph. D in Biochemistry from Baylor University, Texas USA and a Post-doctoral training at the Department of Biochemistry University of Cambridge, UK.Her professional experience as a research scientist, with specialization in the areas of structural biology of plant proteins, protein engineering and analytical biochemistry and food safety, including grass-root work, academic activities, consultation, and positions of institutional leadership spans over three and half decades. She has over 90 peer-reviewed research publications in journals of reputeShe has a long experience as advisor and consultant to the Government in regulatory developments around health and nutrition, food analysis, and harmonization of standards in biotechnology and food safety programs.Presenter:&nbsp;Mr. Nitin G (Product Specialist – Liquid Chromatography &amp; Dissolution, Liquid Phase Division, Agilent Technologies India Pvt. Ltd.)Nitin is an established professional in the analytical industry with over 13 years of experience. He has made significant contributions at Agilent Technologies, where he currently serves as Product Specialist for Liquid phase division supporting our esteemed customers to get the optimal technological solutions in liquid chromatography domain for their laboratory needs and application requisites. With expertise in analytical technologies of Liquid Chromatography and Dissolution testing, Nitin is engaged in various technical knowledge exchange platforms focusing technological advancements, automation of workflows, and lab efficiency enhancement.</blockquote>

Agilent Technologies: Unlock the Possibilities of Food Quality Testing Using Modern Analytical Techniques

Unlock the Possibilities of Food Quality Testing Using Modern Analytical Techniques

In this webinar you will learn the latest trends and regulatory updates in food testing and Agilent latest HPLC & UHPLC series introduction.

Food Quality Testing by Modern Analytical Techniques

Liquid chromatography (LC) is one of the most widely used analytical techniques in the world. With recent initiatives for greener and more sustainable science, the large amounts of hazardous solvent waste generated by analytical scale LC instrumentation have been a topic of interest. By reducing the inner diameter (ID) of the fluidic pathway to the capillary scale (&lt;0.5 mm), separations comparable to their analytical scale counterparts can be performed using several orders of magnitude less solvent (mL down to µL).&nbsp;This presentation will focus on advances in the instrumentation used in the field of capillary LC. Additionally, practical applications of capillary LC will be discussed, including pharmaceutical and illicit drug analysis, food/beverage analysis, and automated reaction monitoring. Large molecule separations will also be featured, including various CQA validations of monoclonal antibodies.<h5>By attending this presentation, you will:</h5><ul><li>Gain insights into the latest advancements in capillary LC</li><li>Understand the environmental benefits of capillary LC and how it can contribute to greener and more sustainable analytical practices</li><li>Discover practical applications of capillary LC</li><li>Explore how capillary LC is used for large molecule separations</li></ul><h5>Who Should View?</h5>Scientists, chemists, analytical researchers, lab directors and managers, VPs and directors of QC/QA, lab owners<blockquote>Presenter: Sam Foster (Ph.D. Student, Department of Chemistry &amp; Biochemistry, Rowan University)Sam Foster is a 5th year Ph.D. student from Rowan University working under Dr. James Grinias. His research has included works on comprehensive two-dimensional liquid chromatography at the capillary scale, novel methods for UV-controlled electrospray ionization, and open-source data acquisition software and hardware. After graduation, he plans to continue in the field, working on furthering capillary scale chromatographic instrumentation.</blockquote>

Separation Science: Compact Capillary Liquid Chromatography: Advances and Applications

Compact Capillary Liquid Chromatography: Advances and Applications

This presentation will focus on advances in the instrumentation used in the field of capillary LC. 

Compact Capillary Liquid Chromatography

Amgen Synthetics Separations Group supports several stages of the pipeline, from early drug discovery to commercial therapeutics. Chromatography technologies such as supercritical fluid chromatography (SFC) and high-performance liquid chromatography (HPLC) have been employed for various purification needs to support the portfolio throughout the years. But trends in drug development have focused on creating more complex and targeted molecules at the same time as a shift in embracing greener, more sustainable research and development practices.&nbsp;Our group has worked to overcome these new challenges by expanding chiral and achiral screening platforms, utilization of software-based method optimization and offline chromatographic modeling, and leveraging mass-directed purification systems for our parallel synthesis libraries. The results are reduced solvent consumption, increased efficiency, and enhanced selectivity for the standard workflows, and a creation of a ugHTPP scale purification platform.<blockquote>Presenter: Kevin Crossley (Scientist, Amgen)Kevin Crossley has worked in the pharmaceutical industry for 23 years and acquired extensive purification and chromatography experience. Kevin graduated from CSUN (California State University of Northridge) in 1998 with a B.A. in Biology with an emphasis in animal physiology. He began his scientific career as a bench chemist and purification scientist performing large scale extractions and isolations of natural products at Miravant Medical Technologies and later found an opportunity to work with Dr. Greg Cauchon, learning analytical techniques for the characterization of polymers and mAbs and renewing his scientific ambitions. In 2008, Kevin moved to Amgen supporting FIH through commercial products with impurity isolations, and large scale (multigram to kilogram) purifications of chiral and achiral molecules by SFC, RPLC and NPLC. In 2019, he joined Larry Miller’s group, utilizing a multitude of analytical and preparative skills to advance the Discovery pipeline and expand the purification capabilities. Kevin is a recipient of the EPA Green Chemistry Award (Greener Reaction Conditions) in the 2017 Green Chemistry Challenge, the Amgen SMP&amp;PD Green Chemistry Award in 2011, patent holder and contributor to several publications.&nbsp;</blockquote>

Agilent Technologies: Evolving Multiscale Separation Capabilities Towards High-Throughput Microgram Purification and Analysis

Evolving Multiscale Separation Capabilities Towards High-Throughput Microgram Purification and Analysis

Amgen Synthetics optimizes drug purification with advanced chromatography, greener practices, and software modeling for greater efficiency and selectivity.

Evolving Multiscale Separation for Microgram Purification

Preparative chromatography has been an integral component of pharmaceutical discovery and development for the past fifty plus years. The past thirty-five years have seen great advances in small molecule purification. Purification techniques have advanced from those centered around large irregular particles operating under low pressure to more efficient high-performance technologies utilizing small spherical particles. Technologies now routinely used include preparative HPLC, SMB, and SFC. At the discovery scale, these advances allow routine and rapid chiral and achiral purification of the large numbers of molecules synthesized at this stage of pharmaceutical R&amp;D. For pharmaceutical development these advances allow chromatographic purifications using HPLC and SMB to be a standard tool in the process development toolbox. The large number of pharmaceutical chiral SMB operations at the manufacturing scale has shown that chromatography can be a cost-effective alternative to asymmetric synthesis or other resolution techniques.This presentation will discuss the critical role small molecule purification plays within a pharmaceutical company. Multiple real-life examples from ug to metric ton scale supporting Discovery through Manufacturing activities will be presented. In addition, the importance of personnel, facility, automation, and information systems for a successfully integrated purification platform will be discussed.<blockquote>Presenter: Larry Miller (Associate Director, Novartis)Larry Miller is an Associate Director in the Global Discovery Chemistry Group at Novartis in Cambridge, MA responsible for analytical and purification support for Discovery. He has spent his career performing small molecule achiral and chiral purifications at the mg to multi-kg scale using a variety of chromatographic techniques. Larry has over 40 peer-reviewed publications and over 45 presentations at scientific meetings. He also co-authored “Modern Supercritical Fluid Chromatography: Carbon Dioxide Containing Mobile Phases” with David Pinkston and Larry Taylor. Larry also serves as co-instructor for an SFC short course which has been held at numerous chromatography conferences in the United States, Europe, and Asia over the past fifteen years. In addition, Larry is the current president of the Green Chemistry Group, a not-for-profit group that organizes the annual SFC conference.</blockquote>

Agilent Technologies: Small Molecule Chromatographic Purification in Pharmaceutical R&D and Manufacturing

Small Molecule Chromatographic Purification in Pharmaceutical R&D and Manufacturing

This presentation will discuss the critical role small molecule purification plays within a pharmaceutical company.

Small Molecule Purification in Pharma R&D and Manufacturing

Join us for this event that will discuss how Shimadzu SFC technology is being used to drive innovation throughout the drug development process. &nbsp;We will examine the break-through use of SFC with LabSolutions MD AQbD method development software to model and optimize peptide separations. &nbsp;The topic of Exposed Polar Surface Area analysis by SFC will be discussed and how its application to cyclic peptide therapeutics speeds up drug candidate selection. &nbsp;We will share recent developments in SFC separations for oligonucleotides, along with other enabling solutions for micro-prep SFC (analytical scale fractionation), hybrid UHPLC-SFC systems, and more.We will host a Q&amp;A session after the presentation to address any questions or comments.<blockquote>Presenter: Brian Domanski (Strategic Collaboration Scientist, Shimadzu Scientific Instruments)Brian has over 10 years working to provide LC and SFC solutions for Pharma, as well as experience with a wide range of chromatography techniques including RP, NP, HILIC, IEX, SEC/GPC, and IC. His focus also includes scalable Informatics and Data Integrity solutions.Presenter: Kevin Wons (Market Manager - Pharma/BioPharma, Shimadzu Scientific Instruments)Kevin has recently joined Shimadzu as Market Manager for Pharma/BioPharma. He has spent the last 6 years at AstraZeneca in the Analytical Sciences Department for product and process characterization for biologics in the AstraZeneca pipeline. Kevin has applications experience with HPLC and LC-MS to do Tryptic Peptide Mapping, Intact Mass Analysis, Subunit Mass Analysis, and Oligosaccharide Profiling. For his last 3 years, he acted as the Glycan Subject Matter Expert (SME) while at AstraZeneca. His work experience also includes Maryland Psychiatric Research Center, where he prepared animal tissue samples such as Brain, Liver, Blood, and Placenta to measure kynurenine pathway metabolites on GC/MS and HPLC. He has a Master’s degree in Professional Studies (MPS) focused on Biotechnology which prepares science professionals to fill management and leadership roles.</blockquote>

Shimadzu: Innovations in SFC Applications and Technology for Pharma and Biopharma

Innovations in SFC Applications and Technology for Pharma and Biopharma

Join us for this event that will discuss how Shimadzu SFC technology is being used to drive innovation throughout the drug development process. 

LCMS webinars focusing on HPLC - page 13