<div><div><div>Scientists at IOCB Prague have developed a breakthrough technology that streamlines aptamer development. The new method integrates several biochemical and bioinformatics steps and paves the way for new diagnostic and therapeutic applications. Ongoing applications include wearable sensors that monitor selected biomarkers in real time and provide early warnings of impending health complications. The findings were published in Nature Communications.</div></div><div>Aptamers are short DNA or RNA strands that can recognize and bind to a specific target molecule with high precision. Similar to antibodies, they can be used to detect these molecules or modulate their activity. Unlike antibodies, they are much more stable, can be produced synthetically, and can be chemically modified to achieve the desired properties. As a result, they can offer capabilities that cannot be achieved with antibodies.</div><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg" alt="IOCHB Prague: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Chemically modified DNA aptamer bound to the target protein." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg 228w," sizes="100vw" width="1343" height="916">As demand grows for accurate and rapid diagnostic tools, aptamers are often better suited to these applications than antibodies. However, developing aptamers is both experimentally demanding and time-consuming. A team of scientists from IOCB Prague, led by Dr. Marek Ondruš and <a target="_blank" rel="noopener noreferrer" href="https://hocek.group.uochb.cz/en">Prof. Michal Hocek</a>, has now developed a technology that significantly shortens the development process.“Developing an aptamer is often like looking for a needle in a haystack. Among trillions of different DNA sequences, we search for the one that binds strongly and specifically to a target molecule. Our new approach first screen for ‘aptamer families’ – hundreds of related sequences – and then identify the best-performing member of the family. This approach can reduce development time from several months to only a few days,” says Marek Ondruš.<blockquote>Video:<a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=_3zXUeVMugU"> New technology streamlines the development of aptamers, synthetic alternatives to antibodies&nbsp;</a></blockquote>The team has also expanded the potential applications of aptamers by chemically modifying their building blocks. For example, introducing functional groups similar to those found naturally in amino acids allows the resulting molecules to combine the advantages of both DNA and antibodies: Their sequence is encoded by the order of nucleotides in DNA, while the attached chemical modifications greatly expand their ability to recognize biologically relevant targets and mimic interactions between proteins that are underlying many biological processes.The researchers demonstrated the potential of this new method using the human insulin receptor, a key protein that regulates blood sugar levels. They developed an aptamer that specifically binds to the receptor and elucidated the molecular basis of this interaction using cryo-electron microscopy. “We have shown that chemically introduced functional groups play a crucial role not only in target recognition and formation of the aptamer–protein complex, but also in stabilizing the DNA aptamer’s structure. Our findings provide new insights into how chemically modified nucleic acids can mimic key properties of proteins,” says Marek Ondruš.In the future, the technology could complement – or, in some applications, even replace – antibody-based diagnostic tools. Researchers are collaborating with <a target="_blank" rel="noopener noreferrer" href="http://iocbtech.cz/">IOCB Tech</a> on its further development, as the company helps to bring discoveries from IOCB Prague into practical applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg" alt="IOCHB Prague/Tomáš Belloň: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Dr. Marek Ondruš, first author of the study, and Prof. Michal Hocek, head of the Bioorganic and Medicinal Chemistry of Nucleic Acids research group at IOCB Prague." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg 234w," sizes="100vw" width="2000" height="1335"><blockquote><h4>Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-73676-y#citeas">Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries</a></h4>ablo Alberto Franco-Urquijo, Marek Ondruš, Jaroslav Kurfürst, Jana Škerlová, Irena Selicharová, Lucie Mužíková Čechová, Hana Šváchová, Alena Semerádtová, Anatolij Filimoněnko, Adéla Fejfarová, Jiří Homola, Tomáš Kouba &amp; Michal HocekNat. Commun., 2026<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41467-026-73676-y">https://doi.org/10.1038/s41467-026-73676-y</a>&nbsp;<h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Discovery of functional nucleic acids from randomized libraries typically relies on multiple, time-consuming iterative rounds of in vitro selection with low success rate. Here, we present a single-round selection strategy for rapid screening of multiple over-represented nucleobase-modified DNA libraries and various selection conditions, capable of identifying high-affinity modified aptamers. Double partition followed by amplification of eluted sequences, NGS analysis and clustering provides fast identification of aptamer candidates. Screening of modified DNA libraries containing modified adenine and uracil nucleotides bearing hydrophobic aromatic phenyl and indole moieties results in development of an aptamer binding human insulin receptor with sub-nanomolar affinity and exquisite specificity. Cryo-EM structure reveals the importance of each aromatic modification, either in stabilizing the secondary structure or facilitating interactions with the protein surface. This approach addresses the main drawbacks of aptamer selection and has potential for high-throughput screening and accelerating the development of next-generation aptamers for diagnostics or therapeutics.</div>

New technology streamlines the development of aptamers, synthetic alternatives to antibodies

Clarity 10.2 expands instrument control capabilities with new control modules and updates the MS extension to help you work more efficiently.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity</a> 10.2 highlights</h4><ul><li>New and updated control modules</li><li>Improved MS data import and export</li><li>Obsolete hardware support ended</li></ul><h4>Instrument control modules</h4>Clarity 10.2 introduces new instrument control modules from Annuo (AS-3117, FAS-3823) and updated instrument controls form Sykam and Ecom.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Instrument_control_modules_5de26782b0.jpg" alt="DataApex: Instrument control modules" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Instrument_control_modules_5de26782b0.jpg 228w," sizes="100vw" width="380" height="260"><h4>MS data import/export improvements</h4>Clarity improves MS data exchange, especially for *.cdf files. MS instruments now support export and import of multiple spectral data and non-spectral signal data in the same CDF file. Import also better handles uniform sampling information, m/z Step values, scale factors, and file names with non-ASCII characters. Exporting individual chromatogram signals from MS instruments is now available through a separate Export Chromatogram dialog.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg" alt="DataApex: MS data import-export improvements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg 228w," sizes="100vw" width="378" height="259"><h4>Obsolete hardware support terminated</h4>Due to changes in Windows driver security requirements, this update discontinues support for obsolete hardware, including INT7, INT9, U-PAD, U-PAD2, and CB20.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg" alt="DataApex: Obsolete hardware support terminated" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg 228w," sizes="100vw" width="378" height="259"><blockquote>Explore the complete list of all changes, fixes, and additions in version 10.2.👉<a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1">See the complete changelog&nbsp;</a></blockquote>

Clarity Chromatography Software 10.2 released!

🎤Dr. Leigh Terry (University of Alabama)In this episode of Concentrating on Chromatography, we sit down with Dr. Leigh Terry from the University of Alabama to explore one of the biggest challenges in environmental analysis today: understanding—and accurately measuring—PFAS in water systems.While most regulatory methods focus on a small number of compounds like PFOA and PFOS, there may be thousands of PFAS compounds in the environment—and much of that burden goes undetected.<h4>In this episode, we cover:</h4><ul><li>Why PFAS are called “forever chemicals” (and why that label is evolving)</li><li>The limitations of targeted LC-MS/MS methods</li><li>What the fluorine mass balance problem reveals about PFAS destruction</li><li>How combustion ion chromatography (CIC) helps measure total organofluorine</li><li>Why “99.99% removal” doesn’t always mean what you think</li><li>Where PFAS actually end up in wastewater treatment systems</li><li>The most effective technologies for PFAS removal today</li><li>Why drinking water may only account for ~20% of total PFAS exposure</li></ul><h4>Key Insight:</h4>Even when analyzing dozens of PFAS compounds, researchers may only be capturing a small fraction of total organic fluorine—raising major questions about how we monitor and regulate these contaminants.<h3>Video Transcription</h3>In this episode of Concentrating on Chromatography, Dr. Leigh Terry discusses PFAS contamination in drinking water, the analytical challenges associated with measuring these compounds, current treatment technologies, and future directions for monitoring and regulation. The conversation highlights how analytical chemistry, environmental engineering, and public health intersect in addressing one of today's most discussed classes of emerging contaminants.<h4>Understanding PFAS and Their Significance</h4><h5>The University of Alabama Water Quality Research Center</h5>Dr. Leigh Terry is part of the University of Alabama's Center for Water Quality Research. The center operates an analytical laboratory focused on drinking water quality and the detection of contaminants at extremely low concentrations.The laboratory specializes in:<ul><li>Drinking water analysis</li><li>Organic contaminants</li><li>Emerging contaminants</li><li>PFAS monitoring</li><li>Water quality research</li></ul>Their work supports both research initiatives and practical solutions for water utilities and environmental agencies.<h4>Why Focus on PFAS?</h4>Although PFAS compounds have existed since the 1940s, public awareness has increased dramatically only in recent years.According to Dr. Terry, several factors contributed to the rapid growth of PFAS research:<ul><li>Increased media attention</li><li>New regulatory initiatives</li><li>Growing evidence of human exposure</li><li>Expanding research opportunities</li><li>Greater understanding of potential health risks</li></ul>PFAS are not new contaminants. Rather, society is only beginning to understand their prevalence in the environment and their potential impacts on human health.<h4>The Development of Standardized PFAS Methods</h4>One major advancement in PFAS analysis has been the development of standardized EPA methods.These methods allow:<ul><li>Consistent testing procedures</li><li>Comparable results between laboratories</li><li>Regulatory monitoring</li><li>Reliable data interpretation</li></ul>Dr. Terry notes that many of these standardized approaches began appearing around 2018–2019, providing a common framework for analytical laboratories and researchers.<h4>How Large Is the PFAS Universe?</h4>Many people are familiar with PFOA and PFOS, two of the most heavily studied PFAS compounds. However, these represent only a small fraction of the broader PFAS family.PFOA and PFOS are often referred to as:<ul><li>Legacy PFAS compounds</li><li>Original PFAS contaminants</li><li>Early commercial PFAS chemicals</li></ul>The challenge is that even minor modifications to molecular structure create entirely new PFAS compounds.Researchers estimate:<ul><li>Thousands of PFAS compounds exist</li><li>Some estimates exceed 10,000 compounds</li><li>Exact numbers depend on how PFAS are defined</li></ul>Scientific debate continues regarding classification and definition, making precise counting difficult.<h4>Why PFAS Are Called "Forever Chemicals"</h4>Dr. Terry notes that the phrase "forever chemicals" has become widely used, although she suggests the terminology could benefit from rebranding.PFAS persistence results from two key chemical properties.<h5>1. Amphiphilic Structure</h5>PFAS molecules contain:<ul><li>A water-loving (hydrophilic) head group</li><li>A water-repelling (hydrophobic) fluorinated tail</li></ul>This unusual combination allows PFAS to partition between:<ul><li>Water</li><li>Solids</li><li>Air-water interfaces</li><li>Foam</li></ul>Unlike many organic compounds, PFAS can interact effectively with multiple environmental phases.<h5>2. Extremely Strong Carbon–Fluorine Bonds</h5>The carbon–fluorine bond is among the strongest bonds in organic chemistry.As Dr. Terry explains:<ul><li>Large amounts of energy are required to break these bonds</li><li>Natural degradation processes are often ineffective</li><li>Destruction can require highly aggressive treatment conditions</li></ul>These properties make PFAS extremely persistent in both environmental systems and biological organisms.<h4>Toxicity, Regulations, and Environmental Behavior</h4><h5>Understanding PFAS Toxicity</h5>One of the biggest challenges in PFAS regulation is the incomplete understanding of toxicity.Dr. Terry explains that EPA regulations generally consider three factors:<ul><li>Presence in drinking water sources</li><li>Toxicity to humans</li><li>Availability of practical treatment options</li></ul>While some PFAS compounds are known to be harmful, many others remain insufficiently studied. Furthermore, PFAS often occur as mixtures rather than individual compounds.This creates important scientific questions:<ul><li>What is the toxicity of individual PFAS?</li><li>What is the toxicity of PFAS mixtures?</li><li>How should cumulative exposure be evaluated?</li></ul>These uncertainties continue to drive active research.<h4>What Happens to PFAS in Wastewater Treatment Plants?</h4>PFAS do not behave like many conventional contaminants.Rather than remaining fully dissolved in water, they often accumulate at interfaces such as:<ul><li>Water-solid interfaces</li><li>Water-air interfaces</li><li>Foam layers</li><li>Biological solids</li></ul>As a result:<ul><li>Some PFAS accumulate in biosolids</li><li>Some partition into foam</li><li>Some remain in treated effluent</li></ul>Their distribution depends on wastewater composition and treatment processes.<h4>Regulatory Monitoring and Targeted LC-MS Analysis</h4>Current EPA regulations focus on targeted analysis of specific PFAS compounds.Examples include:<ul><li>PFOA</li><li>PFOS</li></ul>Drinking water regulations currently establish maximum contaminant levels (MCLs) for these compounds at extremely low concentrations.Advantages of targeted LC-MS analysis include:<ul><li>Clearly defined analytes</li><li>Established regulatory limits</li><li>High analytical sensitivity</li><li>Reliable quantitation</li></ul>However, limitations remain:<ul><li>Only a small subset of PFAS is measured</li><li>Many PFAS compounds remain undetected</li><li>Total PFAS burden may be underestimated</li></ul>As Dr. Terry notes, targeted analysis provides only part of the overall picture.<h4>The Fluorine Mass Balance Problem</h4>One of the most important concepts discussed in the interview is fluorine mass balance.When PFAS degrade, fluorinated fragments may remain in the environment.This creates a critical question:Are treatment technologies truly destroying PFAS, or merely transforming them into shorter-chain PFAS compounds?To answer this, researchers perform fluorine mass balance calculations using:<ul><li>Targeted PFAS analysis</li><li>Fluoride measurements</li><li>Total organic fluorine measurements</li></ul>A successful destruction process should ultimately convert PFAS into fluoride ions rather than simply producing smaller PFAS molecules.<h4>Advanced Analysis, Treatment Technologies, and Future Directions</h4><h5>Combining Targeted and Bulk Fluorine Measurements</h5>Dr. Terry's group complements targeted LC-MS analysis with measurements of total organic fluorine.Approaches include:<ul><li>Absorbable Organic Fluorine (AOF)</li><li>Extractable Organic Fluorine (EOF)</li><li>Combustion Ion Chromatography (CIC)</li></ul>In these methods:<ul><li>Organic fluorine is captured</li><li>Samples are combusted</li><li>Released fluoride is quantified</li></ul>This provides insight into total fluorinated content, although contributions may originate from sources beyond PFAS, including pharmaceuticals and consumer products.<h4>Surprising Findings from Combined Analysis</h4>One important finding from wastewater studies was that targeted PFAS measurements accounted for only a small fraction of total organic fluorine.Dr. Terry reports that:<ul><li>Approximately 4% of measured AOF could be explained by targeted PFAS compounds</li><li>The majority of organic fluorine remained unidentified</li></ul>This suggests that environmental fluorine chemistry is considerably more complex than currently represented by routine monitoring programs.<h4>Preventing PFAS Contamination in the Laboratory</h4>PFAS analysis requires exceptionally strict quality control.Dr. Terry's laboratory routinely:<ul><li>Tests every new batch of SPE cartridges</li><li>Tests carbon tubes before use</li><li>Uses DI water blanks</li><li>Verifies consumables labeled as PFAS-free</li><li>Collects field blanks</li><li>Monitors analytical blanks throughout workflows</li></ul>These practices help prevent contamination from laboratory materials and consumables, a common challenge in ultra-trace PFAS analysis.<h4>Current PFAS Treatment Technologies</h4>Three technologies currently dominate PFAS removal in drinking water treatment:<ul><li>Granular Activated Carbon (GAC)</li><li>Ion Exchange Resins</li><li>Reverse Osmosis (RO)</li></ul>Selection depends on:<ul><li>PFAS concentration</li><li>Water quality</li><li>Local operating conditions</li><li>Utility infrastructure</li></ul>Although many emerging technologies are being investigated, these remain the most widely implemented solutions.<h4>Membrane Filtration and Pretreatment</h4>Dr. Terry collaborates with colleagues studying membrane-based PFAS treatment.Her own work focuses on pretreatment through biological filtration.The strategy involves:<ul><li>Removing natural organic matter</li><li>Reducing membrane fouling</li><li>Improving membrane performance</li><li>Extending operational efficiency</li></ul>This integrated approach demonstrates how multiple treatment technologies can work together to improve PFAS removal.<h4>Regional Monitoring Projects</h4>The University of Alabama team participates in EPA-supported monitoring efforts along the Gulf Coast.Their observations indicate:<ul><li>PFAS rarely occur alone</li><li>Multiple PFAS compounds frequently co-occur</li><li>Contamination often originates from identifiable point sources</li></ul>Examples include:<ul><li>Industrial facilities</li><li>Manufacturing operations</li><li>Military installations</li><li>Carpet manufacturing facilities</li></ul>Source identification remains a critical component of environmental monitoring programs.<h4>Future Directions for PFAS Monitoring</h4>Looking ahead, Dr. Terry sees several opportunities for improvement.Desired advances include:<ul><li>In situ monitoring technologies</li><li>Portable detection systems</li><li>Reduced analytical complexity</li><li>Lower instrument costs</li><li>Less expensive consumables</li><li>Faster analysis workflows</li></ul>Current PFAS testing requires substantial expertise, specialized instrumentation, and rigorous quality control, making widespread implementation challenging.<h4>Dr. Terry's Perspective on Long-Term Solutions</h4>When asked what major PFAS challenge she would most like to solve, Dr. Terry offered a broader perspective.Rather than focusing solely on remediation, she emphasized prevention.Her view is that society should:<ul><li>Better evaluate chemical toxicity before commercialization</li><li>Assess risks before large-scale deployment</li><li>Reduce introduction of harmful compounds into the environment</li><li>Support utilities tasked with contaminant removal</li></ul>She notes that water utilities often bear the responsibility of removing contaminants they did not create, highlighting the need for broader societal and regulatory support.<h4>Final Thoughts</h4>Dr. Terry encourages consumers to:<ul><li>Learn where their drinking water originates</li><li>Read annual water quality reports</li><li>Stay informed about local water quality</li><li>Consider appropriate home filtration where needed</li></ul>At the same time, she emphasizes confidence in municipal water systems and the importance of continued investment in water quality research, monitoring, and treatment technologies.<h4>Conclusion</h4>PFAS remain one of the most complex challenges in environmental chemistry and water treatment. Through advanced analytical methods such as LC-MS/MS, combustion ion chromatography, fluorine mass balance approaches, and large-scale monitoring programs, researchers like Dr. Leigh Terry are helping improve our understanding of these persistent contaminants.The interview highlights that solving the PFAS challenge will require a combination of better analytical tools, improved treatment technologies, stronger regulatory frameworks, and a greater emphasis on prevention before contaminants enter the environment.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

PFAS in Water: Why We’re Missing 96% of the Problem

Article

Product

Video

Scientists

Water

Interview

Science and research

Software

Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

DataApex

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Tosoh Bioscience

Mettler-Toledo

Agilent Technologies

Phenomenex

Waters Corporation

<ul>
<li>Learn the various optical performance parameters</li>
<li>Learn which parameters need to be monitored</li>
<li>Benefits of automated performance qualification</li>
<li>Solution for data integrity compliance in optical instrument qualification</li>
<li>Overview of available service offerings from METTLER TOLEDO</li>
</ul>
During the webinar, we will go into depth about these questions and more:
<h5>What certified reference materials (CRMs) are specified for wavelength accuracy and precision tests by both Pharmacopeias?</h5>
USP: Rare earth oxide solutions/glasses (Ce, Ho, Didymium) or Hg/D2 emission lines
Ph. Eur.: Ho(ClO4)3, Ce2(SO4)3 solutions, or Ho/Didymium glasses, or Hg, D2, Ne, Xe lines
<h5>What CRMs are specified for photometric accuracy and precision tests by both Pharmacopeias?</h5>
USP: for UV range, Nicotinic acid in HCl (6 – 60 mg/L, up to 2.5 A, 213 and 261 nm) or K2Cr2O7 in HClO4 (6 – 200 mg/L, up to 3.0 A, 235, 257, 313, 350 nm); for visible range, neutral density glass filters (up to 3.5 A, 440, 465, 546.1, 590, and 635 nm)
Ph. Eur.: Suitable solid and liquid filters such as certified PDC or neutral density filters; or, instead of certified PDC, Nicotinic acid in HCl.
<h5>What CRMs are specified for photometric linearity tests by both Pharmacopeias?</h5>
USP and Ph. Eur., respectively, specify to use the materials for photometric accuracy tests also for photometric linearity tests.
<h5>Target Audience</h5>
Lab Managers, Lab Technicians, System Owners, Quality Assurance Managers, Quality Assurance Operators
<blockquote>
Presenter: Dr. Fabian Müller (Product Manager SPG UV/VIS)
</blockquote>
<blockquote>
Presenter: Dr. Hans-Joachim Muhr (Business Development Manager BioTech)
</blockquote>

METTLER TOLEDO: Pharmacopeia – What to Check on UV Vis Spectrophotometers? 

Pharmacopeia – What to Check on UV Vis Spectrophotometers? 

USP and Ph. Eur. Requirements Placed on UV/Vis Spectrophotometers

Pharmacopeia – What to Check on UV Vis Spectrophotometers?

Contaminants in the sample matrix can create chromatography issues, system downtime, and unreliable test results. Having clean samples can help prevent most of these problems. There are many options for sample cleanup.
We’ll discuss the following options:
<ul>
<li>Solid phase extraction (SPE), which provides the cleanest sample.
</li>
<li>“Targeted” filtration, which can remove specific interferences from the sample matrix and provides clean samples.
</li>
</ul>
In this talk, we’ll discuss tips for selecting, developing, and implementing SPE methods. We’ll also discuss newly developed targeted filtration options and their benefits.
<blockquote>
Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.
</blockquote>
<blockquote>
Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.
</blockquote>
<blockquote>
Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
</blockquote>

Agilent Technologies: Sample Cleanup: Method Development for Solid Phase Extraction and Beyond

Sample Cleanup: Method Development for Solid Phase Extraction and Beyond

We’ll discuss tips for selecting, developing, and implementing SPE methods. We’ll also discuss newly developed targeted filtration options and their benefits.

Sample Cleanup: Method Development for SPE

Our team of experts will share tips and tricks, dive into practical recommendations and highlight selected applications such as IVT mRNA and long-read sequencing.
Here’s a glimpse of what awaits you:
<ul>
<li>Top tips for achieving high-quality analysis with the Fragment Analyzer and Femto Pulse systems
</li>
<li>Best practices for IVT mRNA analysis using the Fragment Analyzer
</li>
<li>Learn how to achieve a better prediction of long-read sequencing read lengths
</li>
</ul>
Whether you’re a seasoned professional or just starting out, this event promises valuable takeaways!
<blockquote>
Presenter: Samina Kaufmann (Product Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Presenter: Leslie Friedmann (Product Manager, Agilent Technologies)
</blockquote>

Agilent Technologies: Back to the Lab with Your Fragment Analyzer and Femto Pulse

Back to the Lab with Your Fragment Analyzer and Femto Pulse

Top tips for achieving high-quality analysis with the Fragment Analyzer and Femto Pulse systems.

This seminar will guide you through troubleshooting HPLC systems and chromatographic issues using a structured workflow.We will share a novel approach to emphasize the importance of system monitoring, as troubleshooting without monitoring is considerably more challenging. After introducing some key concepts and a troubleshooting workflow, we'll explore practical examples by applying the workflow to failed chromatograms to identify the cause of failure and then use a test cycle to confirm the fix.Who should attend: Analysts working in regulated environments (eg. GLP, GMP) or in non-regulated environments (eg. R&amp;D) who want to troubleshoot their HPLC systems and chromatographic problems effectively.<h4>Key Learnings:</h4>1. The importance of routine system monitoring2. Using qualification checks to diagnose a failure3. Understanding Failure Indicators (HOW the chromatogram failed) vs. Failure Causes (WHY the chromatogram failed)4. Using troubleshooting workflows to quickly identify the failure cause<blockquote>Presenter: Maureen DeLoffi (Senior Product Manager, Waters COrporation)Maureen DeLoffi is a Senior Product Manager at Waters Corporation, where she manages the small molecule portfolio of Analytical HPLC columns. Prior to her Product Manager role, she spent over 12 years as a scientist in the Column Technology Development Lab at Waters. She holds a bachelor’s degree in Mechanical Engineering from the University of New Hampshire.</blockquote>

Waters Corporation: HPLC Troubleshooting: A System Monitoring Approach

HPLC Troubleshooting: A System Monitoring Approach

Troubleshooting HPLC systems and chromatographic issues using a structured workflow.

USP &lt;621&gt; is a crucial chapter in the USP guidelines, affecting around 5000 monographs. It emphasizes the importance of establishing meaningful system suitability criteria for chromatography procedures to ensure valid, compendial, and regulatory compliance. The latest version of USP &lt;621&gt;, effective from December 2022, provides detailed guidance on method adjustments and transfers, facilitating the modernization of existing USP monograph methods. The revisions also expand the scope for modifying gradient method parameters and allow the transition from totally porous silica-based analytical columns to superficially porous particle-based columns to better meet industry needs.<h5>Key Learnings:</h5><ul><li>Strategies for optimizing chromatographic performance using right HPLC system</li><li>Understand the latest updates in USP &lt;621&gt; and how they impact chromatographic practices</li><li>Techniques for enhancing laboratory productivity and reducing operational costs</li><li>Strategies to modernize chromatographic conditions with case-studies</li><li>Revised System suitability requirements and allowable chromatographic system adjustments</li><li>and more</li></ul><blockquote>Presenter: Dr. Manoj Kumar Singh (Senior Vice President, Microlabs Analytical Research Lab)Dr. Manoj K Singh hold over 30 yrs of professional experience of working with global pharma companies leading their Analytical labs to support different pharma work-flows.He holds a Ph.D. degree (Chemistry).He started his professional career in 1993 with Wockhardt and spent significant tenure at major pharma R&amp;Ds like Lupin, Novartis, Orchid, Dabur &amp; Sentiss Pharma.Currently he is associated with MicroLabs Bangalore as Senior Vice President (ADL) leading team of Analytical Chemistry scientists to support formulation development, Validations and method transfer.Presenter: Dr. Kuldeep Sharma (Sales Development Manager - Pharma, Agilent Technologies)Kuldeep Sharma has over 19 years of experience in area/s of Bioanalytical research, Drug metabolism &amp; pharmacokinetics and Pharma regulatory compliance. He has accomplished PhD in Biotechnology. His professional journey covers over 12 years in Analytical research (associated with R&amp;D’s of Zydus Cadila, Lambda, Pliva and Jubilant Biosys) and last 7 years in analytical instruments sales, marketing &amp; business development. With over 21 research articles published in international Journals, He presents convention of continues learning.</blockquote>

Agilent: Enhance Lab Productivity While Meeting Regulatory Requirements: USP 621 Decoded

USP 621 Decoded

Enhance Lab Productivity While Meeting Regulatory Requirements

<h4>Key Learning Points:</h4><ul><li>Identify the common challenges associated with solid phase extraction methods &nbsp;</li><li>Understand and troubleshoot the recovery, contamination and reproducibility issues &nbsp;</li><li>Learn how to find and apply solutions to develop robust and reproducible extraction methods for their sample preparation needs</li></ul><h4>Who Should Attend:</h4><ul><li>Any scientists who wants to learn about solid phase extraction and it’s associated challenges, common troubleshooting steps and solutions to develop robust and reproducible extraction methods for their sample preparation needs</li></ul><blockquote>Presnter: Namrata Saxena (Technical Manager, Phenomenex)Namrata is the Technical Manager for the Asia-Pacific region in Phenomenex and is based in Sydney, Australia. As a member of the global technical team, she is responsible for assisting external customers as well as internal marketing and sales teams of Phenomenex for multifarious technical queries. She specifically support company’s bio-products portfolio through external and internal seminars/webinars, trainings and also contribute in biologics related technical content creation.Before joining Phenomenex she had worked as a Product Specialist and spent several years in stationary phase chemistry, column manufacturing and method development groups in a German company Bischoff Chromatography GmbH.Namrata has a post graduate degree with specialization in Analytical Chemistry. Her competence and focus lies in biotherapeutics characterization through liquid chromatography, antibodies and oligonucleotide chemistry, gene therapy and new bio-therapeutic modalities.</blockquote>

Phenomenex: Solid Phase Extraction Troubleshooting Tips and Tricks

Solid Phase Extraction Troubleshooting Tips and Tricks

Identify the common challenges associated with solid phase extraction methods.

This is the second in our three-part Ask Me Anything webinar series on one of the most frequently asked about topics - processing data. The Components tab in the Processing Method displays many fields of information, giving you options about peak identification, calibration, reference peaks, and fields used in conjunction with custom fields.In Part 2 of this series, we will discuss:<ul><li>Peak identification</li><li>Calibration options including external and internal calibration</li><li>Reference Peaks for peak identification and calibration</li><li>Impurity fields</li><li>CCompRef1, 2, 3, and CConst1-7</li><li>Fields related to chemical structures</li></ul>Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.<blockquote>Presenter: Neil Lander (Principal Product Manager, Informatics, Waters Corporation)Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.</blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Processing Data - Part 2

Empower Tips: Ask Me Anything About Processing Data - Part 2

This is the second in our three-part Ask Me Anything webinar series on one of the most frequently asked about topics - processing data.

Empower Tips: Ask Me Anything About Processing Data

<h5>Who Should Attend:</h5><ul><li>Any HPLC scientists beginning with method development&nbsp;or working with small molecules in pharmaceutical labs &nbsp;</li></ul><h5>Key Learning Points:</h5><ul><li>Learn about selectivity benefits of phenyl phases during reversed-phase HPLC</li><li>Review mobile-phase optimization when using a phenyl column</li><li>Examine a thorough comparison of different phenyl phases with a C18 phase</li></ul><blockquote>Presenter: Zachary Woodward (Senior Technical Specialist, Phenomenex)Zachary Woodward is a Senior Technical Specialist with Phenomenex, Inc, assisting laboratories with method development in the bio-pharma, environmental, and food industries. &nbsp;Zachary spent his formative years developing HPLC and GC methods with a pharmaceutical lab in Albany, NY, after which he developed HPLC and SPE methods in the clinical setting here in Southern California. &nbsp;Zachary received his B.Sci. in Chemistry from the College of William and Mary, was awarded his Master’s in Chemistry at San Diego State University, and is happy to speak with you today!</blockquote>

How to Choose a Phenyl Phase

Learn about selectivity benefits of phenyl phases during reversed-phase HPLC. Review mobile-phase optimization when using a phenyl column.

Are you ready to take your LC-TQ troubleshooting skills to the next level?Whether you’re a seasoned expert or just starting out, this session is packed with valuable insights and practical tips to help you maintain and optimize your system’s performance.<h5>What to Expect:</h5>Unlock the Secrets of LC-TQ: Get a first introduction to troubleshooting and understand why it’s crucial for maintaining optimal performance.<ul><li>Tackle Common Challenges: Learn how to identify and resolve issues like contamination, ion suppression, and clogged nebulizers.</li><li>Preventive Maintenance Tips: Discover best practices for keeping your system in top shape, including the importance of regular check tunes and effective issue reporting.</li><li>Get useful resources to deepen your knowledge about LC-TQ troubleshooting and guides on where to start</li></ul>Come with any questions you might have about troubleshooting your LC/MS Triple Quadrupole system and submit them for the Q&amp;A session.We look forward to welcoming you at this webinar!&nbsp;Presenter: Srinivasa Chakravarthy (Field System Specialist &amp; Certified Instructor (Single and Triple Quad LC/MS), Agilent Technologies)

Agilent Technologies: Troubleshoot Your Misbehaving Triple Quadrupole LC/MS

Troubleshoot Your Misbehaving Triple Quadrupole LC/MS

Are you ready to take your LC-TQ troubleshooting skills to the next level?

Christian Rohrer, Director Sales and Support at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.&nbsp;Analytical scientists are faced with the task of finding the right column from an almost unmanageable range of products. On Tuesday, October 22, Andrea Krumm, Product Manager Analytical Columns at Tosoh Bioscience, will focus on columns that enable protein analysis under native conditions through size exclusion, hydrophobic interaction and ion exchange chromatography. We will highlight the different column characteristics (pore size, particle size, base matrices, column dimensions, ligands) and which questions will help you decide which columns to use.After the webinar, ask your questions during the live Q&amp;A session.<blockquote>Presenter: Andrea Krumm (Product Manager Analytical Columns at Tosoh Bioscience)</blockquote>

Tosoh Bioscience: How to select (U)HPLC columns for protein analysis?

How to select (U)HPLC columns for protein analysis?

Columns that enable protein analysis under native conditions through size exclusion, hydrophobic interaction and ion exchange chromatography

LCMS webinars focusing on Tips & Tricks - page 14

Troubleshoot Your Misbehaving Triple Quadrupole LC/MS

How to Choose a Phenyl Phase

How to select (U)HPLC columns for protein analysis?

Empower Tips: Ask Me Anything About Processing Data - Part 2

Solid Phase Extraction Troubleshooting Tips and Tricks

USP 621 Decoded

Back to the Lab with Your Fragment Analyzer and Femto Pulse

Sample Cleanup: Method Development for Solid Phase Extraction and Beyond

HPLC Troubleshooting: A System Monitoring Approach

Pharmacopeia – What to Check on UV Vis Spectrophotometers?

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