<p><strong>Tasty beer starts with knowing what’s in your hops. Explore how online SPE makes bitter acid analysis in hops faster and more reliable.</strong></p><h4><strong>How can you detect bitter acid profiles quickly, reliably, and more sustainably?</strong></h4><p>For craft brewers and beer lovers alike, a refreshing experience is all about flavor.</p><p>So how can you achieve the desired bitterness in your beer? The answer is not just about the recipe. Finding the right flavor profile starts with the bitter acids present in the starting hops material.</p><div><div><p>Hops (the heart of your favorite beer) are the source of the brew’s distinctive flavor tied to bitterness and aroma.</p><p>At the molecular level, bitterness is driven by <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0308814622016338">α- and β-bitter acids</a>, like humulones and lupulones, whose concentrations determine the final sensory profile of the beer (<strong>Figure 1</strong>).</p><p>Accurately characterizing these compounds in each batch allows brewers to unlock the full potential of every variety of hops.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 1. General chemical structure of hop bitter acids." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg 222w," sizes="100vw" width="700" height="493"></p><p>While conventional analytical methods rely on long, manual sample preparation steps and hazardous solvents, laboratories now strive for greener and more efficient workflows.</p><p>And moving from manual to online sample preparation is one of the easiest ways your lab can reduce waste and — most importantly — improve reliability with automation.</p><h4><strong>Key takeaways</strong></h4><ul><li>Online SPE reduces hands-on work, shortens turnaround time, and supports more sustainable methods by cutting solvent use and eliminating single-use cartridges.</li><li>Automated UHPLC workflows are easier for new users to execute, offering more reproducible quantifications of α- and β-bitter acids in hops.</li><li>Accurate bitter acid profiles help brewers distinguish hop varieties and control flavor with confidence.</li></ul><h4><strong>Comparing offline vs. online solid phase extraction for bitter acid HPLC analysis</strong></h4><p>If you’ve spent long hours in the lab preparing samples for hops analysis, you’re not alone.</p><p>Manual sample preparation often takes the most time in hops analysis, an area where online <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/chromatography-consumables/sample-prep.html">solid phase extraction</a> (SPE) can make a real difference. Here’s why.</p><p>Manual SPE has a lot of manual steps. Extraction. Cartridge loading. Washing. Elution. And sometimes solvent exchange or reduction. All before your actual analysis begins. This labor-intensive workflow can take up to an hour and depends strongly on operator skillsets, making reproducibility a challenge for new hires.</p><h4><strong>Online SPE analysis provides key benefits like:</strong></h4><ul><li>Hands-off workflow free of cartridges, manifolds, or vacuum steps.</li><li>Column washing and re-equilibration occur in parallel with separation.</li><li>SPE step is complete in just two minutes.</li><li>Minimizes operator-dependent variability.</li><li>Uses less than 8 mL of solvent.</li></ul><p>Let’s explore what this technique looks like in practice.</p><h4><strong>Online SPE for analyzing α- and β-bitter acids in hops standard solutions.</strong></h4><p>Changing your sample preparation from manual to automated isn’t complicated. With the right tools you can fully integrate online SPE with your UHPLC system (<strong>Figure 2</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 2. Schematic workflow for the determination of bitter acids in hops. The process involves clean up and instrumental analysis using the Thermo Scientific Vanquish Online SPE UHPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg 245w," sizes="100vw" width="700" height="323"></p><h4><strong>The process for analyzing a hops standard solution is straightforward:</strong></h4><ol><li>First, extract hop samples with ethanol (a greener alternative to toluene or diethyl ether in conventional methods).</li><li>Inject the extract directly into your instrument for analysis. Bitter acids retain<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1970"><strong> on the SPE column</strong></a> while matrix interferences that can comprise quantitation are removed.</li><li>Next, backflush the SPE column to transfer the analytes to the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/423"><strong> analytical column </strong></a>for separation. While this separation is taking place, the SPE column is washed and re-equilibrated in parallel, keeping the total run time to 15 minutes, with the separation of all six major bitter acids in a single analysis (<strong>Figure 3</strong>).</li></ol><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 3. UV chromatogram of hop bitter acids in standard solution (1-cohumulone, 2-humulone, 3-adhumulone, 4-colupulone, 5-lupulone, 6-adlupulone)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg 245w," sizes="100vw" width="700" height="347"></p><h4><strong>Online SPE for analyzing α- and β-bitter acids in real hops samples</strong></h4><p>Analytical labs need methods that are accurate and reliable for brewers to develop distinct and reproducible flavor profiles across batches.</p><p>To assess the applicability of online SPE UHPLC methods for hops analysis, we analyzed bitter acid profiles from four species — Sultana, Barbe Rouge, Azacca, and Sabro.</p><p>The concentration of α-acids dominated all samples, while each hops species exhibited distinct profiles in terms of α-acid and β-acid content, making them suitable for different brewing strategies (<strong>Figure 4</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 4. Bitter acid profiles in four different hop species, error bars display SD (n=6). To check method consistency, we measured each hops extract six times. Variation between measurements was low, with relative standard deviation below 3.5 % across all hops species." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg 245w," sizes="100vw" width="700" height="410"></p><ul><li>For testing labs, this process translates into a workflow that is easy to run, fast, and delivers reliable results without added complexity.</li><li>For craft brewers, reliability means clear, trustworthy insights into hops bitter acid profiles, for better hop selection and more precise control of bitterness and flavor in your beer.</li></ul><h4><strong>From carefully procured samples to tasty sips</strong></h4><p>Efficient UHPLC workflows support faster results, less solvent use, and reliable bitterness data for brewers. And online SPE turns those advantages into practical, day-to-day benefits for brewers and labs alike.</p><ul><li>Less hands-on skills required for lab analysts</li><li>Faster results from labs to brewers</li><li>A more sustainable way to work</li><li>Clear insights into hops flavor</li></ul><p>To learn more about the method and see the full application data, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_004238_hplc_vanquish_online_spe_bitter_acids_hops_cn004238_na_en_587a5d300b.pdf"><strong>check out our application note today</strong></a>.</p><h4><strong>FAQs</strong></h4><div><div><h5><strong>What are the key compounds in hops analyzed by HPLC?</strong></h5><p>Bitter acids, namely α-acids and β-acids, especially the six major bitter acids (humulones and lupulones) that define hop bitterness and are required for quality control and brewing decisions.</p></div><div><h5><strong>What column types are recommended for hop acid separations?</strong></h5><p>Classical C18 Silica columns are ideal for bitter acids separation. UHPLC compatible columns with particle size ≤ 2 µm boost analysis with higher flow rate, thus shorter analysis time and solvent consumption.</p></div><div><h5><strong>What’s the best workflow for hops analysis?</strong></h5><p>A typical workflow for hop analysis consists of three main steps: extraction of the target compounds, clean-up of co-extracted matrix components, and instrumental analysis by HPLC or UHPLC. To keep this workflow efficient and reproducible, you should minimize manual handling and add in automation when possible. Integrating online SPE into your LC instrument allows sample clean-up to run automatically, reducing hands-on time, solvent use, and variability while delivering faster, more reliable results.</p></div></div><blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a>&nbsp;#UHPLC #Automation</p></blockquote></div></div>

How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors

<p><strong>In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</strong></h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul><p>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.</p><h5><strong>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</strong></h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul><p>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.</p><h5><strong>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</strong></h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul><p>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.</p><h5><strong>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</strong></h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul><p>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.</p><h5><strong>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</strong></h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul><p>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.</p><h5><strong>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</strong></h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul><p>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.</p>

Webinars LabRulezLCMS Week 27/2026

<p><strong>The international standard ISO/IEC 17025 [1] clause 7.7.2 with regards to ensuring the validity of results, requires a laboratory to participate in proficiency testing (PT) and/or to participate in interlaboratory comparisons (ILCs) other than PT. However, no guidance as to what these other ILCs might be, or indeed how they might compare in relation to PT, is provided. This leaflet aims to outline other ILCs available, pointing out some of the limitations as compared to PT.&nbsp;</strong></p><p>There are three major types of ILCs in which a group of laboratories analyses identical portions from a homogeneous, stable test sample, with each type being characterized by its intended purpose [2,3]:</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg 245w," sizes="100vw" width="1507" height="607"></p><p>A key point is that PT is an ILC that is designed to specifically evaluate the performance of participants, providing them with an infrastructure to monitor and assess the validity of their results. This is not the key purpose of the two other ILCs, and thus depending on their intended purpose they have a number of limitations that laboratories need to be aware of when using them rather than PT. Only proficiency testing should have been conducted in full compliance to the competency standard ISO/IEC 17043 [4].&nbsp;</p><h4><strong>Evaluation of measurement procedure performance&nbsp;</strong></h4><p>These ILCs, are designed to assess how a particular measurement procedure performs and whether it is fit for its intended purpose. These ILCs are often referred to as a ‘method performance study’ or a ‘collaborative study’ [2]. Some limitations of using such ILCs for monitoring laboratory performance as an alternative to PT are:&nbsp;</p><ul><li>All participants use the same measurement procedure i.e. the measurement procedure that is being validated. Unless the measurement procedure is being routinely used by the participating laboratory such an ILC will not assess the routine performance of the laboratory using their own routine measurement procedure. Nor does it enable them to compare their performance with other laboratories using alternative measurement procedures.&nbsp;</li><li>The measurement procedure is likely to be new, so participants may not have established stable procedures and quality control, so performance may not reflect routine use.&nbsp;</li><li>The report from such an ILC would not provide information on the performance of the participant, if the statistical model used makes the assumption that all laboratories perform with equal variability.</li><li>The design of the study may require the participants to assess multiple samples and/or to undertake multiple replicate analysis on the test materials. This may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li><li>The conclusion of the ILC might be that the measurement procedure being assessed is not valid.&nbsp;</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg 157w," sizes="100vw" width="570" height="566"></p><h4><strong>Characterisation of candidate reference materials&nbsp;</strong></h4><p>These are ILCs that assign a property value to a candidate reference material, usually with a stated measurement uncertainty. These are often referred to as a ‘material certification study’ [2]. Limitations of using such ILCs as an alternative to PT are:&nbsp;</p><ul><li>The analysis of the candidate reference material is likely to require additional care in exercising the routine measurement procedure, such as substantially more replication, and/or may require quite different reporting formats (e.g. all valid replicates instead of a mean result). Thus, such an ILC will not necessarily provide an evaluation of the performance of the laboratory based on the routine use of their measurement procedures.&nbsp;</li><li>The report from such an ILC may include the results from the participating laboratories but would not provide information on the performance of each laboratory.&nbsp;</li><li>Participating laboratories may be required to assess multiple samples and/or to undertake multiple replicate analysis on the candidate reference materials. Thus this may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li></ul><h4><strong>Other ILCs&nbsp;</strong></h4><p>Some other ILCs, whilst not being conducted in full compliance of the requirements of ISO/IEC 17043, may be designed to evaluate participant performance. These would include small ILCs as defined by EA‑2/21 [5] or split sample ILCs i.e. where a portion of a single sample is sent to two or three laboratories. Whilst these ILCs are specifically designed to evaluate participant performance, they may not provide a comprehensive comparison that a PT might provide. In such cases the laboratory needs to evaluate carefully the competence of the organization managing the ILC and the suitability of the service externally provided (ISO/IEC 17025 clause 6.6).</p>

Interlaboratory comparisons other than proficiency testing

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Waters Corporation

<p><strong>With the worldwide growth in the oligonucleotide markets, applying the appropriate tools for oligonucleotide analysis and purification is essential. Ion-paired reverse-phase (IP-RP) liquid chromatography involves the use of ion-pairing reagents to form neutral, hydrophobic complexes with charged oligonucleotides, facilitating their retention and separation on reversed phase columns.</strong></p><p>This presentation introduces the principles and applications of IP-RP in oligonucleotide analysis. It will cover key factors influencing the efficiency of IP-RP methods such as column chemistry, choice of ion-pairing reagent, and mobile phase composition. Additionally, we will discuss method considerations for scaling up your oligonucleotide purification method.</p><blockquote><p><strong>Presenter:</strong> Melissa Goodlad (Application Engineer, Agilent Technologies, Inc.)</p><p>Melissa Goodlad is an Application Engineer providing applications assistance and technical support for Agilent’s HPLC columns and consumables. Before joining Agilent, Melissa was completing her PhD in Physical Chemistry at UC Merced studying nanoparticle self-assemblies.</p></blockquote>

Agilent Technologies: Becoming a Better Chromatographer - Educational Webinar Series

An Introduction to Ion-Paired Reverse-Phase Oligonucleotide Separations: From Analysis to Purification

This presentation introduces the principles and applications of IP-RP in oligonucleotide analysis.

An Introduction to IP-RP Oligonucleotide Separations

<div><div><p><strong>Let's go back to the Basics of Chromatography, Mass Spectrometry, Data Analysis and Trouble-shooting: understanding the importance of the basics will enable you to be more effective in your daily laboratory life.</strong></p><p>This journey started in 2024 and we are now pleased to invite you to join us to go deeper in fundamentals, troubleshooting and application specific topics.</p><h3><strong>EPISODE 3: Fit for Purpose Analysis: How to Develop a Method for Challenging Separations</strong></h3><p><strong>What will you learn?</strong></p><ul><li>Predict the Molecule's Retention in LC</li><li>Select the Critical Parameters during Method Development</li></ul><p><strong>What's next?</strong></p><ul><li>Check out the full Webinar Series Episodes List <a target="_blank" rel="noopener noreferrer" href="https://event.on24.com/eventRegistration/EventLobbyServlet?target=reg20.jsp&amp;eventid=4817889&amp;sessionid=1&amp;key=B9182AE813B3F8A30217606156F96B75&amp;groupId=5901784&amp;sourcepage=register"><strong>here</strong></a></li></ul><p><strong>Not sure to be able to attend live sessions?&nbsp;</strong></p><ul><li>Register today and watch the webinars on-demand</li></ul><blockquote><p><strong>Presenter: </strong>Tobias Langrock (Senior Application Specialist Chemistry, Europe Waters Corporation)</p><p>During his time as a PhD student Tobias discovered his passion for LC and LC-MS/MS and has not lost it since. Joining Waters in 2008 was the perfect opportunity to expand his understanding and expertise in chromatography and mass spectrometry. Since then, he has been involved with a very wide range of instruments and applications, whether small or large molecules, reversed phase LC, IEX or SEC, SFC or GPC. His focus is always on the customers and their daily chromatographic challenges.</p><p><strong>Presenter: </strong>Andrea Gheduzzi (Principal LC Market Dev. Manager, EMEA, Waters Corporation)</p><p>Andrea Gheduzzi, is a principal LC market development manager for EMEA at Waters Corporation. Andrea Gheduzzi has spent more than 30 years in the chromatography field. Across his career he had various roles, service engineer, chromatography lab manager position, and technical manager for chromatography columns. He has mainly been dealing with pharma accounts, also supporting LC method development activities using DoE/QbD approaches. In his previous role as Pharma Business Development Manager at Waters he has further expanded his expertise in AQbD and related applications. Since 2023 he became principal LC market development manager for EMEA and member of the AQbD working party at the EDQM.</p><p><strong>Presenter: </strong>Arjan Timmerman (Pharma Market Development Manager EMEA, Waters Corporation)</p><p>Arjan Timmerman is a market development manager at Waters working in EMEA. Prior to joining Waters in 2014 he worked for 17 years as an Analytical Scientist in the Small Molecule Pharmaceutical industry in various Chemical and Pharmaceutical late-stage development labs. Since 2023 he became QA/QC market development manager for EMEA working in a dedicated team and focuses on Pharmaceutical workflows, AQbD, Impurities, LC Chromatography and Compliance.</p></blockquote></div></div>

Waters Corporation: Back to Basics Advanced Webinar Series 2025

Back to Basics Advanced - Pharma Impurities: Separations

Predict the Molecule's Retention in LC. Select the Critical Parameters during Method Development.

<div><div><h3><strong>Oligos, Oligos, Oligos!</strong></h3><p><strong>Oligonucleotide therapeutics and reagents are everywhere. They are helping the world address previously untreatable diseases, and they are also being used in new ways to sensitively quantify and sequence important translational research samples. Whether it be a CRISPR guide RNA, lipid conjugated anti-sense oligonucleotide, or a specially modified assay probe, these molecules must be tested to ensure they have the right sequence, pinpoint-specific modifications, conjugate moieties and purities. &nbsp;And when dosed as a therapeutic, they need to be carefully quantified to confirm pharmacokinetic models. &nbsp;</strong></p><p>Join the Waters Chromatography and LC-MS Team and their collaborators from MRIGlobal, Regeneron, and Acuitas Therapeutics in this special episodic, 3 week webinar series to learn more about the science of oligos and the power of LC-based approaches to analyze them.&nbsp;</p><h3><strong>EPISODE 3</strong></h3><h4><strong>Part 1: Deformulation and Nucleic Acid Quantification in LNPs Using Size-Exclusion Chromatography</strong></h4><p>Learn about an alternative high-throughput approach for the deformulation and quantification of nucleic acid payloads in lipid nanoparticles (LNPs). Using a low adsorption 2.5 µm SEC 450 Å Column, this size-exclusion chromatography (SEC) method employs deformulating conditions with detergent and organic solvents in the mobile phase to disrupt LNPs without prior sample treatment.</p><blockquote><p><strong>Presenter: </strong>Mateusz Imiolek, PhD (Waters Corporation, Geneva, Switzerland)</p><p>Mateusz joined Waters in 2022 where he has been studying challenges associated with characterizing gene therapeutics and ensuring that new Waters products are fit for this purpose. Before that, he completed his academic training in bioorganic chemistry, which allows him to apply detailed molecular insights to ensure the newly developed columns and assays are carefully designed for such analytes.</p></blockquote><h4><strong>Part 2: High-Throughput Nucleic Acid and Lipid Adduct Quantification in LNPs Using Ion-Paired Reverse-Phase Chromatography</strong></h4><p>A high-throughput LC-based assay developed for nucleic acid quantification in lipid nanoparticles (LNPs). By using a short, low adsorption 2.5 µm BEH C18 column, the assay allows for the efficient separation of LNP payloads, including guide RNA and mRNA, from detergent-disrupted samples. The assay is designed for excellent robustness and reproducibility, making it suitable for routine application.</p><blockquote><p><strong>Presenter: </strong>Razvan Cojocaru (Research Scientist II, Acuitas Therapeutics)</p><p>Razvan has been a research scientist in the Analytical department at Acuitas Therapeutics since 2021, where he focuses on developing new methodology for characterizing nucleic acids in LNP therapeutics. Prior to Acuitas, Razvan completed his studies at Simon Fraser University in Molecular Biology and Biochemistry, where he focused on Ribozyme and RNA Aptamer in vitro selection and optimization.</p></blockquote></div></div>

LC for LNP RNA Molecular Composition Analysis

Join Waters Chromatography, LC-MS Team & collaborators for a 3-week webinar series on oligos science & LC-based analysis.

<p><strong>Oligonucleotides are highly important biopharmaceuticals with strongly increasing relevance. Due to the high similarity of impurities originating from the synthesis, however, purification can be challenging.</strong></p><p>This seminar demonstrates a successful large scale purification of an oligonucleotide using anion exchange chromatography (AEX) as well as efficient quality control by applying ion-pairing reversed phase chromatography (IP-RP).</p><h4><strong>Content</strong></h4><ul><li>How to perform a large scale AEX purification: Design of preparative system and useful features</li><li>Benefits and limitations using AEX for preparative purification</li><li>What to consider for developing an IP-RP methods: selection of stationary phase, ion-pair reagent, and solvent conditions</li><li>How to improve analytical results by utilizing bioinert column hardware</li></ul><h4><strong>Who are the seminar for?</strong></h4><ul><li>Advanced and beginners interested in peptides and/or oligonucleotides.</li></ul><blockquote><p><strong>Presenter: </strong>Dr. Ulrike Krop (KNAUER Wissenschaftliche Geräte GmbH)</p><p><strong>Presenter: </strong>Dr. Daniel Eßer (YMC Europe GmbH)</p></blockquote>

KNAUER: Purification and quality control of oligonucleotides

Purification and quality control of oligonucleotides

This seminar demonstrates a successful large scale purification of an oligonucleotide using anion exchange chromatography (AEX).

<p><strong>Liquid chromatography (LC) is one of the most widely used analytical techniques in the world. With recent initiatives for greener and more sustainable science, the large amounts of hazardous solvent waste generated by analytical scale LC instrumentation have been a topic of interest. By reducing the inner diameter (ID) of the fluidic pathway to the capillary scale (&lt;0.5 mm), separations comparable to their analytical scale counterparts can be performed using several orders of magnitude less solvent (mL down to µL).&nbsp;</strong></p><p>This presentation will focus on advances in the instrumentation used in the field of capillary LC. Additionally, practical applications of capillary LC will be discussed, including pharmaceutical and illicit drug analysis, food/beverage analysis, and automated reaction monitoring. Large molecule separations will also be featured, including various CQA validations of monoclonal antibodies.</p><h5><strong>By attending this presentation, you will:</strong></h5><ul><li>Gain insights into the latest advancements in capillary LC</li><li>Understand the environmental benefits of capillary LC and how it can contribute to greener and more sustainable analytical practices</li><li>Discover practical applications of capillary LC</li><li>Explore how capillary LC is used for large molecule separations</li></ul><h5><strong>Who Should View?</strong></h5><p>Scientists, chemists, analytical researchers, lab directors and managers, VPs and directors of QC/QA, lab owners</p><blockquote><p><strong>Presenter:</strong> Sam Foster (<i>Ph.D. Student, Department of Chemistry &amp; Biochemistry,</i> <i>Rowan University)</i></p><p>Sam Foster is a 5th year Ph.D. student from Rowan University working under Dr. James Grinias. His research has included works on comprehensive two-dimensional liquid chromatography at the capillary scale, novel methods for UV-controlled electrospray ionization, and open-source data acquisition software and hardware. After graduation, he plans to continue in the field, working on furthering capillary scale chromatographic instrumentation.</p></blockquote>

Separation Science: Compact Capillary Liquid Chromatography: Advances and Applications

Compact Capillary Liquid Chromatography: Advances and Applications

This presentation will focus on advances in the instrumentation used in the field of capillary LC. 

Compact Capillary Liquid Chromatography

<p><strong>Preparative chromatography has been an integral component of pharmaceutical discovery and development for the past fifty plus years. The past thirty-five years have seen great advances in small molecule purification. Purification techniques have advanced from those centered around large irregular particles operating under low pressure to more efficient high-performance technologies utilizing small spherical particles. Technologies now routinely used include preparative HPLC, SMB, and SFC. At the discovery scale, these advances allow routine and rapid chiral and achiral purification of the large numbers of molecules synthesized at this stage of pharmaceutical R&amp;D. For pharmaceutical development these advances allow chromatographic purifications using HPLC and SMB to be a standard tool in the process development toolbox. The large number of pharmaceutical chiral SMB operations at the manufacturing scale has shown that chromatography can be a cost-effective alternative to asymmetric synthesis or other resolution techniques.</strong></p><p>This presentation will discuss the critical role small molecule purification plays within a pharmaceutical company. Multiple real-life examples from ug to metric ton scale supporting Discovery through Manufacturing activities will be presented. In addition, the importance of personnel, facility, automation, and information systems for a successfully integrated purification platform will be discussed.</p><blockquote><p><strong>Presenter: </strong>Larry Miller (Associate Director, Novartis)</p><p>Larry Miller is an Associate Director in the Global Discovery Chemistry Group at Novartis in Cambridge, MA responsible for analytical and purification support for Discovery. He has spent his career performing small molecule achiral and chiral purifications at the mg to multi-kg scale using a variety of chromatographic techniques. Larry has over 40 peer-reviewed publications and over 45 presentations at scientific meetings. He also co-authored “Modern Supercritical Fluid Chromatography: Carbon Dioxide Containing Mobile Phases” with David Pinkston and Larry Taylor. Larry also serves as co-instructor for an SFC short course which has been held at numerous chromatography conferences in the United States, Europe, and Asia over the past fifteen years. In addition, Larry is the current president of the Green Chemistry Group, a not-for-profit group that organizes the annual SFC conference.</p></blockquote>

Agilent Technologies: Small Molecule Chromatographic Purification in Pharmaceutical R&D and Manufacturing

Small Molecule Chromatographic Purification in Pharmaceutical R&D and Manufacturing

This presentation will discuss the critical role small molecule purification plays within a pharmaceutical company.

Small Molecule Purification in Pharma R&D and Manufacturing

<p><strong>Join us for this event that will discuss how Shimadzu SFC technology is being used to drive innovation throughout the drug development process. &nbsp;We will examine the break-through use of SFC with LabSolutions MD AQbD method development software to model and optimize peptide separations. &nbsp;</strong></p><p>The topic of Exposed Polar Surface Area analysis by SFC will be discussed and how its application to cyclic peptide therapeutics speeds up drug candidate selection. &nbsp;We will share recent developments in SFC separations for oligonucleotides, along with other enabling solutions for micro-prep SFC (analytical scale fractionation), hybrid UHPLC-SFC systems, and more.</p><p>We will host a Q&amp;A session after the presentation to address any questions or comments.</p><blockquote><p><strong>Presenter: </strong>Brian Domanski (Strategic Collaboration Scientist, Shimadzu Scientific Instruments)</p><p>Brian has over 10 years working to provide LC and SFC solutions for Pharma, as well as experience with a wide range of chromatography techniques including RP, NP, HILIC, IEX, SEC/GPC, and IC. His focus also includes scalable Informatics and Data Integrity solutions.</p><p><strong>Presenter: </strong>Kevin Wons (Market Manager - Pharma/BioPharma, Shimadzu Scientific Instruments)</p><p>Kevin has recently joined Shimadzu as Market Manager for Pharma/BioPharma. He has spent the last 6 years at AstraZeneca in the Analytical Sciences Department for product and process characterization for biologics in the AstraZeneca pipeline. Kevin has applications experience with HPLC and LC-MS to do Tryptic Peptide Mapping, Intact Mass Analysis, Subunit Mass Analysis, and Oligosaccharide Profiling. For his last 3 years, he acted as the Glycan Subject Matter Expert (SME) while at AstraZeneca. His work experience also includes Maryland Psychiatric Research Center, where he prepared animal tissue samples such as Brain, Liver, Blood, and Placenta to measure kynurenine pathway metabolites on GC/MS and HPLC. He has a Master’s degree in Professional Studies (MPS) focused on Biotechnology which prepares science professionals to fill management and leadership roles.</p></blockquote>

Shimadzu: Innovations in SFC Applications and Technology for Pharma and Biopharma

Innovations in SFC Applications and Technology for Pharma and Biopharma

Join us for this event that will discuss how Shimadzu SFC technology is being used to drive innovation throughout the drug development process. 

Innovations in SFC Applications in (Bio)Pharma 

<div><div><p><strong>The random appearance of unknown peaks (ghost peaks), contaminant peaks, loss of sensitivity or lack of reproducibility are not uncommon in chromatography. Determining the root cause of these issues can be time-consuming and difficult to troubleshoot. In many cases, the root cause is determined to be the sample vials.&nbsp;</strong></p><p>In this webinar we’ll delve into the critical role of chemically tested sample vials, ensuring accurate and reliable analytical outcomes. Discover how seemingly minor details—such as vial septum material, dimensions, and the glass surface can significantly impact your results. Our experts will guide you through real-world cases where choosing the right vial made all the difference.</p><h4><strong>Here’s what you’ll learn:</strong></h4><ul><li>Understand the vial characteristics that directly influence your analytical data.&nbsp;</li><li>Explore Waters’ range of sample vials designed to address common challenges.&nbsp;</li><li>Learn practical tips for diagnosing and preventing vial-related issues in chromatography.&nbsp;</li></ul><blockquote><p><strong>Presenter: </strong>Markus Wanninger (Principal Product Manager, Waters Corporation)</p><p>Markus is the principal product manager of sample vials and plates. He has a degree in chemical engineering and long-standing experience in the pharmaceutical industry. Markus has a keen understanding of the needs of analytical HPLC laboratories, and the controls required to have certainty in results and staying in compliance. The drive to control and optimize processes enabled the Markus and the vials team to drive continuous improvement of product lines to meet the demands of todays’ laboratories. Outside his professional life Markus is a passionate photographer and an inspiring chef and pitmaster.</p><p><strong>Presenter: </strong>Nikunj Tanna (Principal Scientist, Waters Corporation)</p><p>Nikunj Tanna is a Principal Scientist at Waters Corporation who is passionate about meeting people, making connections, and learning from every conversation. His role at Waters focuses on complete workflow solutions for quantitation of small and large molecules. These end-to-end workflows include automation, novel sample preparation and chromatographic separation approaches, followed by detection using TQ and/or HRMS platforms. In his current role, he has particular emphasis on sample preparation and automation for bioanalysis, ADME and DMPK applications. In previously roles, he lead a clinical diagnostics team at Berg LLC developing bioanalytical methods for drug candidates. He graduated with a BS in Biotechnology and MS in Bioanalytical Sciences from the University of Mumbai, and MS in Pharmaceutical Sciences from Northeastern University.</p></blockquote></div></div>

Waters Corporation: Are your sample vials causing problems in your HPLC analysis?

Are your sample vials causing problems in your HPLC analysis?

Discover how seemingly minor details—such as vial septum material, dimensions, and the glass surface can significantly impact your results. 

Are your sample vials causing problems in your HPLC analysis

<p><strong>Whether you are working in standard analytical lab, where unknown impurities are the biggest challenge or you are working in discovery labs where you need all the possible isolates for your further characterization or you are working nutraceuticals, natural product extract or working in R&amp;D department, or peptide/ Oligonucleotide synthesis. You need the most flexible, efficient purification / preparative LC systems and columns for the best outcomes.</strong></p><p>Any scientists working on Preparative HPLC or Purification system have few of the following questions to be addressed:</p><ul><li>Struggling with accurate &amp; precise compound purification?</li><li>Do you get the best recoveries of your isolation compounds?</li><li>Are your purification systems future upgradable?</li><li>Are your purification systems tailor made to your needs?</li></ul><p>Explore how Agilent purification systems help you support purification from analytical and full-scale purification system.</p><p>Join us to know the latest the developments in Agilent Purification / Preparative systems.</p><h4><strong>Who Should Attend?</strong></h4><ul><li>Scientists working on Preparative HPLC or Purification system</li><li>Lab Heads of Synthetic R&amp;D, CRO, CDMO&nbsp;</li><li>Lab Scientists working on Synthetic peptide or Oligonucleotide compounds and impurities isolation<br>&nbsp;</li></ul><blockquote><p><strong>Presenter: </strong>Mr. Sambasivarao Vattikuti (Pre-Sales Application Engineer, Agilent Technologies India Pvt. Ltd.)</p><p>Sambasivarao holds a Master of Pharmacy degree from SRM University, Chennai, and has 15 years of industry experience. He spent a decade working in New Product Development and Analytical Research &amp; Development at leading pharmaceutical companies such as Syngene and MicroLabs. Currently, he serves as an Application Engineer at Agilent Technologies, specializing in Chromatography, Dissolution, and Laboratory Informatics, with five years of experience in this role. His areas of expertise include Chromatography, Quality by Design (QbD), and Purification techniques.</p></blockquote>

Agilent Technologies: Everyday Purification & Isolation with Confidence

Everyday Purification & Isolation with Confidence

Explore how Agilent purification systems help you support purification from analytical and full-scale purification system.

<h4><strong>Key Learning Points</strong></h4><ul><li>Find how best to utilize modern UHPLC particles to optimize chromatographic performance.</li><li>Learn when to consider particle morphologies as a method development tool.&nbsp;</li><li>Learn when to consider phase selectivity as a method development tool.&nbsp;</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>Any LC User who is interested in the performance benefits, and differences, of both core- shell and full porous particles.</li></ul><blockquote><p><strong>Speaker: </strong>Sean Orlowicz (Principal Market Development Manager - Pharmaceutical, Phenomenex)</p><p>Sean Orlowicz has been working in the field of analytical chemistry for over 23yrs. &nbsp;After receiving his degree in Chemistry from the University of Illinois at Chicago, he started his career in large scale synthesis, using HPLC and GC techniques in reaction monitoring and purification. &nbsp;Sean took this experience to Phenomenex in 2003, &nbsp;working on site with clients worldwide on method development, troubleshooting and method transfer. &nbsp;Sean explored instrumentation for a few years, growing to specialize in LCMS, before returning to Phenomenex to help build and manage Phenomenex’s new applications laboratory. &nbsp;Since then, Sean has been driving innovation and growth though Business Development efforts, and most recently, in 2025 Sean has taken a Market Development Management role, spearheading our global efforts in Pharmaceuticals.</p></blockquote>

Phenomenex: Symbiosis of Core-Shell and Fully Porous Particles

Symbiosis of Core-Shell and Fully Porous Particles

In this webinar you will find how best to utilize modern UHPLC particles to optimize chromatographic performance.

LCMS webinars focusing on HPLC - page 14