<div><p><strong>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</strong></p></div><div><div><div><div><h3><strong>What is Protein Purification?</strong></h3><p>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.</p><p>This process is especially vital in biotechnological areas inside immunology and microbiology. In <strong>immunology</strong>, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In <strong>microbiology</strong>, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</p></div></div></div></div><div><div><div><div><h3><strong>Types of Protein Purification Methods</strong></h3></div></div></div></div><div><p>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</p></div><div><div><div><div><h4><strong>Non-Chromatographic Approaches</strong></h4></div></div><div><p>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.</p><h5><strong>Precipitation Techniques</strong></h5><p>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:</p><ul><li><strong>Organic solvent precipitation</strong> involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li><strong>Isoelectric point precipitation </strong>exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li><strong>Nonionic polymer precipitation</strong> uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5><strong>Dialysis</strong></h5><p>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.</p><h5><strong>Immunocapture (Magnetic Bead-Based Purification</strong></h5><p>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:</p><ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul><p>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.</p><h5><strong>Centrifugation</strong></h5><p>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.</p><p><strong>Preparative Ultracentrifugation</strong><br>Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.</p><p><strong>Differential Centrifugation</strong><br>Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.</p><p><strong>Rate-zonal Gradient Centrifugation</strong><br>This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.</p><p><strong>Isopycnic Centrifugation (Equilibrium Centrifugation)</strong><br>Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</p></div></div></div><div><div><div><div><h4><strong>Chromatographic Approaches</strong></h4><p>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.</p><h5><strong>Ion Exchange Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526"><strong>Luna SCX </strong></a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540"><strong>Biozen WCX</strong></a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5><strong>Size Exclusion Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.</p><p>Phenomenex offers a range of <strong>Biozen columns for SEC</strong> separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534"><strong>Biozen dSEC-2</strong></a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"><strong> Biozen dSEC-7 </strong></a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.</p><p>For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533"><strong>Biozen SEC-2</strong></a><strong> </strong>and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532"><strong>Biozen SEC-3</strong></a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.</p><h5><strong>Reversed-Phase Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.</p><p>The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.</p><h5><strong>Hydroxyapatite Chromatography</strong></h5><p>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.</p><p>Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.</p><h5><strong>Hydrophobic Interaction Chromatography</strong></h5><p>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.</p><h5><strong>Affinity Chromatography</strong></h5><p>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.</p><p>This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</p></div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3><span><strong>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</strong></span></h3><p><span><strong>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.</strong></span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"></p><h4><span><strong>1. Improper Filter Handling</strong></span></h4><p><span>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.</span></p><ul><li><span><strong>Solution:</strong> Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</span></li></ul><h4><span><strong>2. Selecting an Inappropriate Pore Size</strong></span></h4><p><span>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.</span></p><ul><li><span><strong>Solution:</strong> A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</span></li></ul><h4><span><strong>3. Choosing the Wrong Membrane Material</strong></span></h4><p><span>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.</span></p><ul><li><span><strong>Solution:</strong> Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</span></li><li><span><strong>Pro Tip:</strong> For additional guidance, see our blog article <strong>"How to Choose the Right Membrane for your Syringe Filter"</strong>, which provides a detailed comparison of membrane materials and their recommended applications.</span></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"></p><h4><span><strong>4. Ignoring Filter Capacity</strong></span></h4><p><span>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.</span></p><ul><li><span><strong>Solution:</strong> When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</span></li></ul><h4><span><strong>5. Overlooking Sample Adsorption</strong></span></h4><p><span>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.</span></p><ul><li><span><strong>Solution:</strong> For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</span></li></ul><h4><span><strong>6. Failing to Optimize Filtration Performance</strong></span></h4><p><span>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.</span></p><ul><li><span><strong>Solution:</strong> If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</span></li></ul><h4><span><strong>Final Thoughts</strong></span></h4><p><span>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.</span></p><p><span>With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627"></p><p><span><strong>Happy filtering!</strong></span></p>

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

Article

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Tosoh Bioscience

Thermo Fisher Scientific

SelectScience

VWR/Avantor

Waters Corporation

<p><strong>PhenoAcademy Webinars</strong></p>
<p> <strong>Liquid Chromatography Series</strong></p>
<h4><strong>HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns</strong></h4>
<ul>
<li>Recognize columns exhibiting comparable selectivity to your current column selection</li>
<li>Identify columns with different or orthogonal selectivity to your current column of choice</li>
<li>Understand the resources that are available for selectivity data for HPLC columns</li>
</ul>
<blockquote>
<p><strong>Presenter</strong>: Kevin Zhao (Senior Technical Specialist)</p>
</blockquote>
<blockquote>
<p>Kevin Zhao is the Senior Technical Specialist with Phenomenex and has 5 years of experience in biochemistry research; 4 years of Protein/Cancer research with CSULA-City of Hope Cancer Collaborative and over 9 years in Phenomenex. Mr. Zhao is a member of a global Technical Support Team which includes providing problem-solving, troubleshooting, and applications assistance for customers worldwide in the fields of chromatography (liquid and gas) and sample preparation, and also handling Industries supported include – pharmaceutical and biopharmaceutical, clinical research and clinical toxicology, food safety, environmental, fuels and specialty chemicals. Mr. Zhao received his B.S. in Biology at the California State Polytechnic University, Pomona. earned his M.S. in Biochemistry at California State University, Los Angeles, and also earned a MBA at the University of the Incarnate Word.</p>
</blockquote>
<blockquote>
<p><strong>Presenter</strong>: Koen Askamp (Technical Specialist)</p>
</blockquote>
<blockquote>
<p>Koen is from the Netherlands, studied Molecular Life Sciences at Wageningen University (Netherlands), majoring in immunology. Analytical Chemistry was one of my favorite courses – who doesn’t love solving puzzles?</p>
</blockquote>
<blockquote>
<p>I like drumming, scuba diving, motorcycle-riding, and this past winter I learned how to ski (a little)!</p>
</blockquote>
<blockquote>
<p><strong>Presenter</strong>: Namrata Saxena (Technical Manager)</p>
</blockquote>
<blockquote>
<p>Namrata Saxena is a Technical Manager - Asia/Pacific” with Phenomenex and is based in Sydney, Australia. She has a post graduate degree with a specialization in Analytical Chemistry, with extensive experience in the Liquid chromatography domain, and her current focus is in Omics and Biologics field. </p>
</blockquote>
<blockquote>
<p>Before joining Phenomenex she had worked as “Product Specialist” and spent several years in column chemistry, column manufacturing and method development in the application and manufacturing labs of Bischoff Chromatography, Germany. </p>
</blockquote>
<blockquote>
<p>In her current role, she provides product selection, method development, training and troubleshooting support to customers as well as Phenomenex Sales Consultants globally.  </p>
</blockquote>


Phenomenex: PhenoAcademy Webinars - Liquid Chromatography Series

HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns

Recognize columns exhibiting comparable selectivity to your current column selection.

Identification of Equivalent and Orthogonal HPLC Columns

<p><strong>Aggregate analysis of Adeno Associated Virus (AAV) is crucial for quality assessment and currently presents various challenges including long run time, high volume sample consumption where samples are scares and over all requires reliable technique to address all aspects related to quality and throughput.</strong></p>
<p>In this webinar, the speakers will focus on optimizing a high throughput SEC method using a novel size exclusion chemistry to reduce sample consumption and  run time. Additionally, method versatility with multiple mobile phase, multiple detectors across various AAV serotypes will be explored. </p>
<h5><strong>Key Learning Points:</strong></h5>
<ul>
<li>Learn about a novel Size exclusion chemistry that can improve lab throughput and reduce sample consumption for AAV analysis</li>
<li>Find new method optimization tips and tricks to maximize AAV Aggregate separation</li>
<li>Understand how optimizing particle chemistry, pore size and hardware can improve Aggregate separation in Size Exclusion Chromatography of AAVs</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Shilin Cheung, PhD (Sr. Research Scientist, Phenomenex)</p>
</blockquote>
<blockquote>
<p>Dr. Cheung received her BS (2006) and Ph.D. (2012) in Bioanalytical Chemistry from the University of Toronto. She has developed, qualified, and validated innovative analytical methods in CE based microfluidics, automated low volume UV-vis spec., and UHPLC assays. Her methods have been applied to manufacturing process developments, formulation and stability and DS/DP characterization and specification testing. At the Phenomenex Innovation Campus she is focused on developing fit for purpose analytical methods to help advance developments in gene therapeutics.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Ramkumar Dhandapani, PhD (Senior Product Manager, Phenomenex)</p>
</blockquote>
<blockquote>
<p>Dr. Dhandapani has been in the chromatography industry for over 20 years with hands-on and troubleshooting experience. He has earnt a Masters and PhD degree in Analytical chemistry from Seton Hall University with specialization in Microextractions, Multidimentional Chromatography and Tandem Mass Specs techniques. He has developed and validated several regulatory compliant methods in Pharmaceutical, Food, Fuels and Environmental industry as well as incorporated method improvement and troubleshooting across a range of separation techniques. Dr. Dhandapani joined Phenomenex in August of 2014 and he serves as a Senior Product Manager at Phenomenex. In addition to managing the product line, Dr. Dhandapani presents on innovations in chromatography at various chromatography conference.</p>
</blockquote>


Phenomenex: Overcoming Challenges with AAV Aggregate Analysis Using a Novel Size Exclusion Particle

Overcoming Challenges with AAV Aggregate Analysis Using a Novel Size Exclusion Particle

Optimization of high throughput SEC method using a novel size exclusion chemistry to reduce sample consumption and  run time

AAV Aggregate Analysis Using a Novel Size Exclusion Particle

<p><strong>HPLC and LC-MS method development can be challenging, especially for complex samples. Whether the complexity comes from the sample itself, or the sample matrix, there are different approaches that can be taken to help resolve more peaks in these types of sample.</strong></p>
<h4><strong>1. A practical guide to maximize the sample peak capacity for complex small molecule separations</strong></h4>
<ul>
<li>Dr. Arianne Soliven – Product Specialist, Avantor, UK</li>
</ul>
<p>Method development for complex small molecule samples using reversed-phase (RP) separation conditions is a challenging problem, especially when the sample matrix itself may contain many unknown analytes in a dynamic range of different concentrations. This talk is based on guiding analysts on an established approach published in 2013 aimed at optimizing the practical method decisions: (column length (L), temperature (T), flow rate (F), and final organic strength mobile phase composition (∅final) to maximize the separation’s peak capacity.</p>
<h4><strong>2. Software-assisted method development enables the optimization of chromatographic separation and enhancement of peak capacity</strong></h4>
<ul>
<li>Dr. Imad Haidar Ahmad - Research Director, Amgen, USA</li>
</ul>
<p>Software-assisted method development is a powerful tool to achieve optimal performance in chromatographic separations. In the pharmaceutical industry, chromatographic modelling is a fundamental part of the analytical workflow, which focuses on chromatography column selection along with optimizing mobile phase composition and gradient conditions. This integration of chromatography modelling into analytical workflow enables accelerating method development and ensures compliance to regulatory requirements. In this presentation, we show how chromatography modelling can be used to pick the optimum selectivity, shorten cycle time, decrease peak width, and enhance resolution, which is translated into higher peak capacity. We will highlight the use of modelling to increase peak capacity and address challenging separations by showcasing several real-life methods employed in the pharmaceutical industry.</p>
<h4><strong>3. Strategies for trace contaminant analysis in complex sample matrices</strong></h4>
<ul>
<li>Dr. Andrés Pérez-Parada - Professor at the University of the Republic, Uruguay</li>
</ul>
<p>Various types of organic pollutants can be present in both food and environmental samples. This presentation aims to showcase the practical applications of LC-FLD in the routine analysis of glyphosate in freshwater and agricultural soils. Additionally, rapid determination of Ochratoxin A in wines will be demonstrated, employing advanced core-shell Avantor LC columns. Furthermore, the presentation will delve into the environmental dissipation of rice pesticides, presenting large-scale multi-residue methods using LC-MS/MS. This overview will give insights into the analytical versatility and efficacy of LC in addressing diverse challenges related to organic pollutant analysis.</p>


Avantor: Different Perspectives and Strategies for Liquid Chromatography Peak Capacity Optimization and Method Development

Different Perspectives and Strategies for Liquid Chromatography Peak Capacity Optimization and Method Development

LC Peak Capacity Optimization and Method Development

<p><strong>In this live webinar, we will introduce the brand new Thermo Scientific™ Hypersil GOLD™ Peptide LC Column, designed for all peptide separation, including excellent retention of hydrophilic peptides and separation of deamidated species in a single run. This column is ideal for the development and manufacturing of biopharmaceuticals, achieving minimal RSD% and impeccable lot-to-lot consistency.</strong></p>
<p>Following a product presentation of this advanced technology, you will get a chance to <strong>hear testimonies</strong> and <strong>results from our customers</strong> and early-adopters in the field. Finally, our experts will proceed to dive deeper into the related applications, such as <strong>peptide mapping and monitoring</strong> as part of the <strong>Multi-Attribute Method</strong> (<strong>MAM</strong>), to provide you with the solutions to resolve all your peptide challenges. We hope to see you there!   </p>
<p><strong>Learning Points:</strong></p>
<ul>
<li><strong>Introduction to the new Hypersil GOLD Peptide LC Column</strong></li>
<li>Hear from early adopters about their use of this advanced technology</li>
<li>Dive deeper into the <strong>related applications to resolve all peptide challenges</strong></li>
</ul>
<p><strong>Who should attend?</strong></p>
<ul>
<li>Biopharmaceutical research scientists</li>
<li>Analytical scientists from biopharma companies working on or interested in peptide mapping and monitoring</li>
<li>PhD students and scientists looking to advance their knowledge of peptide mapping and monitoring</li>
</ul>
<p><strong>Short notice:</strong></p>
<p>Can&#39;t attend the live broadcast? No problem! Register anyway and you will automatically receive the link to the webinar recording. </p>
<blockquote>
<p><strong>Presenter:</strong> Serdar Bilgesoy (Product Manager Premier LC columns, Thermo Fisher Scientific)</p>
</blockquote>
<blockquote>
<p>As the Global Product Manager for LC Columns at Thermo Fisher Scientific, Serdar brings over 15 years of experience in analytical instruments across various roles in product management, sales, marketing, application, and service responsibilities. He has specialized in segments such as Life Sciences, Clinical, Pharma, Biopharma, and Forensics. He is an experienced Application, Sales, and Marketing Executive in HPLC, LC-MS/MS, LC-MS/TOF, and MALDI-TOF instruments, possessing sophisticated marketing knowledge, a vast sales network, technical background, and innovative solutions expertise.</p>
</blockquote>


ThermoFisher Scientific: Resolve all your peptide challenges: deep dive into new separation technology and applications

Resolve all your peptide challenges: deep dive into new separation technology and applications

We will introduce the brand new Thermo Scientific™ Hypersil GOLD™ Peptide LC Column, designed for all peptide separation.

Peptide challenges: new separation technology

<p><strong>By attending this eSeminar, you will:</strong></p>
<ul>
<li>Gain practical insights into optimizing sample preparation in alignment with EPA Method 1633’s regulatory requirements by the use of dual-phase SPE cartridges, covering background analysis, MDL studies, and recoveries</li>
<li>Benefit from real-world experiences from Complete Environmental Testing, offering valuable insights on navigating EPA 1633 in an end-to-end workflow, while utilizing dual-phase SPE cartridges to improve laboratory efficiency</li>
<li>Understand key details for successful PFAS analysis</li>
<li>Learn how to optimize analysis for ultra-short chain PFAS compounds</li>
<li>See how to expand the range of PFAS compounds to analyze in a single run</li>
</ul>
<h4><strong>Event Overview</strong></h4>
<p>Separation Science, in collaboration with its sponsors Waters and Restek, is pleased to bring you an upcoming online eSeminar focusing on advances in PFAS analysis. This unique event will bring together key experts in the field and provide an interactive learning environment for scientists working in this industry.</p>
<h4><strong>Presentations</strong></h4>
<p><strong>Efficiency in EPA Method 1633: Validation and Implementation Strategies</strong></p>
<p><em>by Jeffrey Smith, Director of Operations, Complete Environmental Testing (CET)</em></p>
<p>Join us for a presentation focused on optimizing EPA Method 1633 in your laboratory highlighting the validation and implementation of dual-phase WAX and GCB cartridges. EPA 1633 establishes a method for the determination of 40 PFAS from challenging matrices such as non-potable waters, soils, biosolids, and tissue. For these matrix types, sample preparation has moved beyond the use of a WAX cartridge alone. GCB has been added to the sample preparation workflow to provide additional sample cleanup. We will discuss the full EPA 1633 workflow, including topics such as background analysis for soils and water, MDLs achieved, observed recovery in authentic samples, and the practical advantages of dual-phase cartridges in reducing manual labor steps compared to loose GCB in EPA 1633.</p>
<p>This presentation is designed for scientists, laboratory managers, and others looking to implement and validate EPA Method 1633 or optimize their sample preparation process in their current workflow. Join us as Complete Environmental Testing shares experiences on working with the complex matrices in EPA 1633 and breaks down advantages in their method workflow with the use of dual-phase SPE cartridges.</p>
<p><strong>Untapped Ultrashort-chain PFAS Analysis Combined with Routine PFAS Analysis in a Single LC-MS/MS Run</strong></p>
<p><em>by Mike Chang, Market Development Manager, Restek Corporation</em></p>
<p>Starting from measuring C8 many decades ago, today’s PFAS testing requires a lot more chromatography capabilities when an extended list of PFAS analysis is desired. Chain length ranging from C1 to C18 and a functional group changing from carboxylic acids to ethers, sulfonamides, and more, with multiple oxygens in the structure, can be challenging for a chromatograph all in one run. In this presentation, we will see examples of a combined PFAS panel analyzed in one run with chain length from C1 and above, in addition to AFFF and FTS compounds.</p>
<h4><strong>What you need to know:</strong></h4>
<p><strong>Date</strong>: 16 April, 2024</p>
<p><strong>Start times</strong>:</p>
<ul>
<li><p>Broadcast 1: 9 am BST (London) / 10 am CEST (Paris/Berlin) / 1:30 pm IST (New Delhi) / 4 pm SGT (Singapore)</p>
</li>
<li><p>Broadcast 2: 10 am PDT (Los Angeles) / 12 pm CDT (Chicago) / 1 pm EDT (New York)</p>
</li>
</ul>
<p><strong>Duration</strong>: Approximately 90 minutes</p>
<blockquote>
<p><strong>Presenter:</strong> Jeffrey Smith (Director of Operations, Complete Environmental Testing (CET))</p>
</blockquote>
<blockquote>
<p>Jeffrey Smith holds a BS in Chemistry from Quinnipiac University. He has been working in environmental analytical chemistry for 18 years, with experience in several disciplines, including GC/MS and LC-MS/MS. Jeffrey now serves as the director of operations at Complete Environmental Testing. For the past few years, his focus as director has been building the PFAS department at CET from the ground up, including method development and researching LC/MS/MS instrumentation for the laboratory. Jeffrey serves on several PFAS work groups and is an active member of the Reasonable Confidence QA/QC Protocols work group for the state of Connecticut.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Mike Chang (Market Development Manager, Restek Corporation)</p>
</blockquote>
<blockquote>
<p>With an analytical chemistry background and 15+ years of experience in the chromatography industry, from instrumentation to applications, Mike is the key liaison to testing agencies and industries, bringing better ways of measuring contaminants in the environment and food. He has been chairing PFAS sessions and presenting PFAS topics at various conferences.</p>
</blockquote>


Advances in PFAS Analysis

Understand essential details for successful PFAS analysis and optimize EPA Method 1633 in your lab.

<p>Christian Rohrer, Director Sales and Marketing at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.</p>
<p><strong>On Tuesday, April 16, Corey Meadows, Product Specialist Bioanalysis at Tosoh Bioscience, dives into the analysis of antibody-drug conjugates and their drug-antibody ratio by hydrophobic interaction chromatography.</strong></p>
<p>Antibody-drug conjugates (ADCs) are dual-action cancer biotherapeutics, where monoclonal antibodies carrying tumor-killing payloads specifically bind to cancer cells and accelerate cancer cell death. Characterizing the ratio of tumor-killing payload to cancer-binding antibody (DAR) is a critical quality attribute needed to determine the potential potency of a given ADC candidate. This webinar features HIC as a solution for quantifying DARs in ADC biotherapeutics. An overview of ADC and HIC fundamentals will be presented, targeting analytical method development scientists seeking insights for mobile-phase and stationary-phase selections for separating variable conjugated ADC variants.</p>
<p>After the webinar, ask your questions during the live Q&amp;A session. </p>
<blockquote>
<p><strong>Presenter:</strong> Christian Rohrer (Director Sales and Marketing at Tosoh Bioscience GmbH)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Corey Meadows (Product Specialist Bioanalysis at Tosoh Bioscience)</p>
</blockquote>


Tosoh Bioscience: Tosoh Tuesday - Hydrophobic Interaction Chromatography as a Platform for Characterizing ADCs

Hydrophobic Interaction Chromatography as a Platform for Characterizing ADCs

Hydrophobic Interaction Chromatography as a Platform for Characterizing Antbody-Drug Conjugates

HIC for Characterizing ADCs

<h4><strong>European Free Educational Webinar Series</strong></h4>
<p><strong>Let&#39;s go back to the basics of Chromatography, Mass Spectrometry, Data Analysis and Trouble-shooting: understanding the importance of the basics will enable you to be more effective in your daily laboratory life.</strong></p>
<h4><strong>EPISODE 1</strong></h4>
<p><strong>Sample Preparation - Theory and Overview</strong></p>
<p><strong>What will you learn?</strong></p>
<ul>
<li>Goals and available Techniques</li>
<li>Intro to Solid Phase Extraction (SPE)</li>
</ul>
<h4><strong>What&#39;s next?</strong></h4>
<p>Full Webinar Series Episodes List is available <a href="https://event.on24.com/wcc/r/4551012/F69479929A905500D8B21508527807E5/5385652"><strong>here</strong></a></p>
<p>Back to Basics - Sample Preparation: Theory and Overview</p>
<ul>
<li>Tuesday, May 07, 2024, 11:00 AM CEST</li>
</ul>
<p>Back to Basics - Sample Preparation: Tips &amp; Tricks to make it simple</p>
<ul>
<li>Wednesday, June 05, 2024, 11:00 AM CEST</li>
</ul>
<p>Back to Basics - Theory of Liquid Chromatography</p>
<ul>
<li>Wednesday, July 03, 2024, 11:00 AM CEST</li>
</ul>
<p>Back to Basics - Basics of Liquid Chromatography</p>
<ul>
<li>Tuesday, September 10, 2024, 11:00 AM CEST</li>
</ul>
<p>Back to Basics - Analytical Method Development</p>
<ul>
<li>Wednesday, October 02, 2024, 11:00 AM CEST</li>
</ul>
<p>Back to Basics - The importance of Compliance</p>
<ul>
<li>Tuesday, October 22, 2024, 11:00 AM CEST</li>
</ul>
<p>Back to Basics - Introduction to Mass Spectrometry</p>
<ul>
<li>Wednesday, November 13, 2024, 11:00 AM CET</li>
</ul>
<p>Back to Basics - Troubleshooting your LC and MS Systems</p>
<ul>
<li>Tuesday, December 10, 2024, 11:00 AM CET</li>
</ul>
<p><strong>Not sure to be able to attend live sessions?</strong></p>
<p>Register today and watch the webinars on-demand</p>
<blockquote>
<p><strong>Presenter:</strong> Xavier Rodriguez (Principal Sales Application Specialist, Waters)</p>
</blockquote>


Waters Corporation: Back to Bacisc Free Educational Webinar Serries

Sample Preparation: Theory and Overview

The first episode will introduce you into goals and available techniques of Solid Phase Extraction (SPE).

<h5><strong>Overview:</strong></h5>
<ul>
<li>The reporting limit of a method is also dependent upon the consistency of clean blanks and elimination of sporadic contamination.</li>
<li>Vendors of PFAS consumables need to provide certified, trace level PFAS free products.</li>
<li>PFAS are everywhere!  They are widely used, vendors/suppliers when contacted don&#39;t know their processes are using them.</li>
<li>Since PFAS contamination has become an issue with PFAS analysis all field samples are taken in duplicate, not just field blanks. </li>
<li>The complete data package and replicates need to be evaluated by the user in order to make sound conclusions and identify false positives. </li>
<li>Every step there is a chance to incorporate “free-range” PFAS in your sample, minimize the preparation steps</li>
<li>How PFAS is analyzed at EPA Region 5 Laboratory</li>
</ul>
<h5><strong>Who Should Attend:</strong></h5>
<ul>
<li>Anyone lab scientists, or managers involved in PFAS Analysis</li>
</ul>
<h5><strong>Key Learning Points:</strong></h5>
<ul>
<li>Learn how to approach analyzing for PFAS, it is not rocket science like they made it out to be</li>
<li>Become aware of Sporadic PFAS Contamination</li>
<li>Understand how to identify false positives</li>
<li>Require all analysis quality control and samples to be analyzed in replicates to identify false positives.</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Larry Zintek (Organic Methods Group Leader, UA EPA)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Maryam Sayeed (Product Management Specialist, Phenomenex)</p>
</blockquote>


Phenomenex: Identifying and Avoid False Positives During Routine PFAS Analysis

Identifying and Avoid False Positives During Routine PFAS Analysis

Learn how to approach analyzing for PFAS, it is not rocket science like they made it out to be.

False Positives During Routine PFAS Analysis

<p><strong>Since 2018 when the presence of Nitrosamines was first reported in several ARB (angiotensin II receptor blocker) drugs, there has been increasing regulatory focus to mitigate the presence of these potential human carcinogens in drug substances and drug products.</strong></p>
<h5>Who Should Attend:</h5>
<ul>
<li><p>Anyone with an interest in, or challenged by, the analysis N-Nitrosamines and Nitrosamine Drug Substance Related Impurities (NDSRI) in API and/or Drug Products</p>
</li>
<li><p>Analysts and Scientists working in the Pharmaceutical and Generic Pharmaceutical industries.</p>
</li>
</ul>
<h5>Key Learning Points:</h5>
<ul>
<li><p><strong>Review  the latest regulatory updates (FDA and EMA)</strong></p>
</li>
<li><p><strong>Learn about published LC-MS/MS applications which address the N-Nitrosamines and NDSRI analysis challenges in drug products</strong></p>
</li>
<li><p><strong>Find solutions to sample preparation challenges for N-Nitrosamines analysis in drug products</strong></p>
</li>
<li><p><strong>Obtain examples of specific Phenomenex applications for nitrosamines (10 mutagenic nitrosamine impurities in Acyclovir) and NDSRIs (N-nitroso propranolol in a propranolol drug substance and product)</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Phil Koerner (Market Development Manager, Pharmaceuticals)</p>
</blockquote>


Phenomenex: Nitrosamines in Drug Products: Where Are We Now?

Nitrosamines in Drug Products: Where Are We Now?

Learn about published LC-MS/MS applications which address the N-Nitrosamines and NDSRI analysis challenges in drug products

<h4><strong>PhenoAcademy Webinars Sample Preparation Series</strong></h4>
<p><strong>Dealing with Proteins and Phospholipids: Advances using Simplified Liquid Extraction</strong></p>
<ul>
<li>Understand the challenges associated with matrices from clinical samples </li>
<li>Take a deep dive into a number of approaches to overcome the presence of proteins and lipids in the samples</li>
<li>Learn more about the methods through case studies on blood and urine</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Laura Ward (Technical Specialist)</p>
</blockquote>
<blockquote>
<p>Laura Ward is a Technical Specialist for the Asia-Pacific region with Phenomenex, located in New Zealand. She completed an Honours thesis in the field of Geochemistry at the University of Sydney, Australia where she discovered a love for Analytical Chemistry. Following her academic pursuits, Laura joined Phenomenex Australia as a Technical Sales Consultant where she supported various industries and laboratories. After 5 years and a move to New Zealand, she has joined the Phenomenex Technical Team where she supports scientists globally with their method development, troubleshooting and application support.  In her spare time Laura enjoys being outdoors in the New Zealand countryside and cooking from scratch, most recently venturing into cheesemaking!</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Lucia Geis Asteggiante (Technical Specialist)</p>
</blockquote>
<blockquote>
<p>Lucia is a Senior Technical Specialist that has recently joined Phenomenex’s Global Technical Team. She has a broad experience in the field of Analytical Chemistry with a particular focus on LC chromatography coupled with mass spectrometry. Lucia comes from a strong academic and regulatory background with experiences ranging from method development and analysis of small molecules to large protein complexes. She gained her Ph.D. at the University of Maryland - College Park, USA and did her post-doctoral work at the University of Oxford, UK. During her time as a researcher, she has authored/co-authored 23 scientific publications and 2 book chapters.</p>
</blockquote>


Phenomenex: Dealing with Proteins and Phospholipids: Advances using Simplified Liquid Extraction

LCMS webinars focusing on Consumables - page 14