Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

High-performance liquid chromatography (HPLC) remains one of the most widely used analytical techniques for separating, identifying, and quantifying compounds in complex mixtures. Successful HPLC method development depends not only on selecting the correct column and optimizing the mobile phase composition, but also on choosing the appropriate detector. Even a perfectly optimized chromatographic separation provides little value if the detection system cannot properly capture and interpret the analytes of interest.In many ways, the detector acts as the “vision system” of the chromatograph. It converts separated compounds into measurable electronic signals, allowing analysts to identify components and determine their concentrations. The choice of detector therefore has a major impact on sensitivity, selectivity, robustness, and the overall quality of analytical data.This article provides an overview of the most common HPLC detection techniques, discusses their strengths and limitations, and outlines the most important criteria to consider when selecting a detector for a specific analytical task.<h3>HPLC Detection Technologies</h3>HPLC detectors are typically divided into two major categories:<ol><li>Specific detectors (solute-property detectors)</li><li>Bulk property detectors</li></ol>Specific detectors respond to a particular chemical or physical property of the analyte. Ideally, their signal is largely unaffected by changes in mobile phase composition. Common examples include UV/Vis detectors, fluorescence detectors, and mass spectrometers.Bulk property detectors, on the other hand, measure differences between the pure mobile phase and the mobile phase containing analytes. These detectors are considered more universal because they respond to properties shared by many compounds. Refractive index detectors are among the best-known examples, alongside conductivity and light-scattering detectors.Both detector categories have important advantages depending on the application, sample type, and analytical goals.<h3>Common Detector Types Used in HPLC</h3>A wide variety of detectors are available for HPLC systems, ranging from highly universal devices to extremely selective and sensitive technologies.Common detector options include:<ul><li>UV/Vis detectors</li><li>Diode array detectors (DAD/PDA)</li><li>Fluorescence detectors</li><li>Refractive index detectors (RID)</li><li>Conductivity detectors</li><li>Evaporative light scattering detectors (ELSD)</li><li>Charged aerosol detectors (CAD)</li><li>Mass spectrometry detectors (MS)</li></ul>Each technology offers a different balance of sensitivity, selectivity, robustness, operational complexity, and cost.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg" alt="KNAUER: Detector overview with characteristics. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg 245w," sizes="100vw" width="1293" height="596"><h3>Selecting the Right HPLC Detector</h3>There is no universally ideal detector for all HPLC applications. The best choice depends on several factors, including analyte chemistry, matrix complexity, required detection limits, and instrument compatibility.Careful detector selection is essential for obtaining reliable and meaningful analytical results.The following considerations can help guide the selection process.<h4>1. Chemical and Physical Properties of the Analytes</h4>The chemical nature of the compounds being analyzed is one of the most important selection criteria.Questions to consider include:<ul><li>Do the analytes contain chromophores?</li><li>Are the compounds naturally fluorescent?</li><li>What is their polarity?</li><li>What is their molecular weight?</li><li>Are they volatile or non-volatile?</li></ul>Compounds containing UV-active groups are often well suited for UV/Vis detection, while fluorescent molecules can benefit from the high sensitivity of fluorescence detectors. Non-UV-active compounds may require universal detection approaches such as CAD, ELSD, or mass spectrometry.<h4>2. Required Sensitivity</h4>Detector sensitivity determines the minimum concentration that can be reliably detected and quantified.Routine quality control analyses often rely on UV/Vis detectors because they provide sufficient sensitivity for many applications while remaining relatively simple and cost-effective. However, trace-level analysis or highly complex matrices may require more advanced technologies such as:<ul><li>Fluorescence detection</li><li>Charged aerosol detection</li><li>Mass spectrometry</li></ul>These techniques offer significantly lower detection limits and greater analytical specificity.<h4>3. Detector Linearity</h4>Linearity describes how well detector response correlates with analyte concentration across a defined range.A detector with strong linearity ensures accurate quantification and simplifies calibration procedures. Poor linearity can introduce quantification errors, especially when analyzing samples across broad concentration ranges.<h4>4. Dynamic Range</h4>Dynamic range refers to the concentration interval over which the detector can reliably measure analytes.Applications involving samples with highly variable concentrations require detectors capable of maintaining accurate response over a broad range. A wide dynamic range reduces the need for repeated dilutions or multiple analytical runs.<h4>5. Selectivity</h4>Selectivity defines the detector’s ability to distinguish target analytes from other matrix components.This factor becomes particularly important for complex samples containing many interfering substances. Highly selective detectors reduce the risk of overlapping signals and false positives.Mass spectrometry is often advantageous when dealing with unknown compounds or highly complicated sample matrices because it provides structural information in addition to quantitative data.<h4>6. Budget Considerations</h4>Detector cost is another practical factor that laboratories must evaluate.UV/Vis and refractive index detectors are generally among the more economical choices, making them attractive for routine analyses and standard applications.More sophisticated technologies such as fluorescence detection, CAD, or mass spectrometry typically require:<ul><li>Higher initial investment</li><li>Additional maintenance</li><li>More complex infrastructure</li><li>Greater operational expertise</li></ul>However, the overall value of the detector should always be considered in relation to analytical performance requirements and long-term application needs.<h4>7. Instrument and Mobile Phase Compatibility</h4>The selected detector must be compatible with the operational conditions of the HPLC system.Important parameters include:<ul><li>Flow rate range</li><li>Pressure limitations</li><li>Mobile phase composition</li><li>Solvent compatibility</li></ul>Some detectors are highly sensitive to mobile phase composition or gradient changes, while others tolerate a broader range of operating conditions.Ensuring proper compatibility helps prevent baseline instability, signal interference, and reduced detector performance.<h4>8. Destructive vs. Non-Destructive Detection</h4>Another important consideration is whether the analytical workflow involves only detection or also preparative purification.Non-destructive detectors allow collected fractions to remain intact after detection, which is highly beneficial for preparative chromatography and purification workflows.Destructive detectors, by contrast, consume or alter the sample during measurement and are therefore more suitable for analytical applications where sample recovery is not required.<h4>9. Reliability and Ease of Operation</h4>Practical usability is often just as important as analytical performance.Some detectors are designed for straightforward plug-and-play operation, while others require:<ul><li>Frequent calibration</li><li>Specialized maintenance</li><li>Dedicated gas supplies</li><li>Vacuum systems</li><li>Advanced operator expertise</li></ul>Laboratories should evaluate the available technical support, user experience, and maintenance capabilities before implementing more sophisticated detection technologies.Reliable operation and manageable maintenance requirements can significantly improve laboratory productivity and reduce downtime.Choosing the correct HPLC detector starts with understanding both the sample and the analytical objective. Rather than selecting a detector simply because it is available, laboratories should ensure that the technology aligns with the chemistry of the analytes, required performance characteristics, and workflow demands.In many cases, combining multiple detectors within a single HPLC system can provide even greater analytical insight by delivering complementary information from the same chromatographic separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg" alt="KNAUER: Flow scheme for detector selection. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg 245w," sizes="100vw" width="1316" height="808">Interested in learning more? Future articles will explore individual HPLC detection techniques in greater detail, including their operating principles, application areas, and practical advantages for different analytical workflows.

A comprehensive guide for selecting the appropriate HPLC detector

News from LabRulezLCMS Library - Week 20, 2026

Article

Product

Application

Scientists

Science and research

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Agilent Technologies

Phenomenex

Restek Corporation

SelectScience

VWR/Avantor

Waters Corporation

In preparative HPLC method development, our goal is the separation and isolation of a valuable compound. Throughput, yield, and purity of the compound are the important parameters for preparative HPLC.
In this webinar, we will discuss the process of scaling up from an analytical to a preparative HPLC method. We will also look at column considerations to increase sample throughput using superficially porous particles in preparative LC columns to drive speed and efficiency.
<blockquote>
Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.
</blockquote>

Scale Up with Confidence: Column Selection for Preparative HPLC

In this webinar, we will discuss the process of scaling up from an analytical to a preparative HPLC method.

Scale Up: Column Selection for Preparative HPLC

The emergence of NPS (novel psychoactive substances) on the illicit drug market has created a challenge for toxicology laboratories. With the constant rise of novel compounds, keeping a laboratory testing scope up-to-date is proving increasingly difficult.
This webinar will provide a background on NPS as well as strategies and tools, such as Restek’s Pro EZLC Chromatogram Modeler, that can be used to effectively add NPS compounds to a laboratory’s testing scope using Restek’s Raptor Biphenyl column.
<ul>
<li>Learn key information on how to effectively analyze NPS (novel psychoactive substances) compounds.</li>
<li>See how the Pro EZLC Chromatogram Modeler can help with adding these compounds to your analysis.</li>
<li>Learn how Raptor Biphenyl allows for easy incorporation of these compounds into existing methods.</li>
</ul>
<blockquote>
Presenter: Haley Berkland (LC Applications Scientist)
</blockquote>

Restek Corporation: Strategies for Adding Xylazine, Nitazenes, and Other NPS to Laboratory Testing Scopes

Strategies for Adding Xylazine, Nitazenes, and Other NPS to Laboratory Testing Scopes

We will provide a background on NPS as well as strategies and tools, such as Restek’s Pro EZLC Chromatogram Modeler...

Strategies for Adding Xylazine, Nitazenes, and Other NPS

Key Learning Points:
<ul>
<li>Learn why chiral screening is done, what are the benefits</li>
<li>Take a deep dive into columns, and screening modes/methods</li>
<li>Listen into sample compatibility considerations including: detection, solubility, and chiral centers</li>
<li>Understand quick, easy, and cost-effective ways to identify a solution for your chiral separation needs</li>
</ul>
Who Should Attend:
<ul>
<li>Any LC users who would like to get tips on a chiral screening process for method development</li>
<li>Any academia or pharmaceutical scientists who need to rapidly develop a method for separation and characterization of their chiral compounds</li>
</ul>
<blockquote>
Presenter: Zandra Baja (Applications Scientist, Phenomenex)
</blockquote>

Phenomenex: Chiral Chromatography Screening 101

Chiral Chromatography Screening 101

Join us in this upcoming webinar and take a deep dive into columns and screening modes/methods!

<h5>Key learning objectives:</h5>
<ul>
<li>What is chiral chromatography, chiral screening, why do it, what are the benefits...</li>
<li>What the chiral screening process looks like</li>
<li>Scope, deliverables, and timelines for chiral screening</li>
<li>How to request chiral screening services</li>
</ul>
<blockquote>
Presenter: Zandra Baja (Applications Scientist)
</blockquote>
<blockquote>
Zandra is an Applications Scientist at PhenoLogix specializing in chiral screening, USP and PhEur applications, and environmental GC-MS applications. Zandra obtained her bachelor&#39;s degree in chemistry from California State University of Long Beach. Prior to joining Phenomenex in 2021, she spent 3 years working as a chemist in the petroleum industry and 8 years as an analytical chemist working with gas chromatography in an environmental air testing laboratory. Zandra has also spent several years in quality assurance management where she was responsible for maintaining ISO/IEC 17025 and TNI Standard laboratory accreditations. 
</blockquote>

What is chiral chromatography, chiral screening, why do it, what are the benefits.... What the chiral screening process looks like....

As a continuation of our Chromatography of Bioseparations 101 series, we bring to you 2 NEW SESSIONS in our Chromatography of Bioseparations 102 webinar series! Join us live for our new parts 6 and 7. If you haven&#39;t had an opportunity to register for Parts 1-5, feel free to regster and listen to them on-demand now.
These new webinars will focus on the analytical separation of biomolecules such as peptides and amino acids.
Reverse-phase chromatography is the predominant technique used for the analyses and or purification of chemically synthesized peptides or peptides generated by the enzymatic digestion of proteins (i.e., peptide mapping). It is advantageous to have a simplified step-by-step approach to develop a method that can be applied to chemically synthesized peptides or protein digests.
Amino acid analysis is required in several different areas of research and is a fundamental tool in various product analytical activities. Due to the varied properties of amino acids (i.e., acidic, basic, etc.), the separation and detection represents an analytical challenge and depending on throughput, sample preparation can be laborious. 
Who should attend?
<ul>
<li>Those interested in a refresher on foundational principles and tips of reverse phase separations of peptides.</li>
<li>Those interested in how to select columns and mobile phases that are optimal for peptide and amino acid workflows.</li>
<li>Those looking for an overview of industry advancements and new offerings.</li>
</ul>
<h3>PART 6 | June 20th, 2023 | 10:30AM – 12:00PM EDT</h3>
<h3>PRINCIPLES AND PRACTICAL STEPS IN DEVELOPING REVERSE-PHASE PEPTIDE SEPARATIONS</h3>
<ul>
<li>Learn how column chemistry and mobile phase affects peptide separation and selectivity.</li>
<li>Review the factors that synergistically work to obtain desired resolution of peptides contained in a complex mixture.</li>
</ul>
<blockquote>
Presenter: Bill Warren (Consulting Product Manager – Consumables and Lab Automation, Waters Corporation)
</blockquote>
<blockquote>
Bill received his MS in Microbiology and Immunology from the University of Louisville School of Medicine. In 1986, joined Waters Corporation as an senior applications chemist involved with the research and development of HPLC, FPLC, and Capillary Electrophoresis applications for the analysis and purification of proteins, peptides, and nucleic acids. Following four years as a field based technical sales representative for Waters, Bill enjoyed a tenure as Manager, Core Product Evaluation Laboratory within the Waters Worldwide Marketing Organization. Bill currently is a Principal Bioseparation Columns Product Manager whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of proteins and peptides.
</blockquote>

Waters Corporation: PRINCIPLES AND PRACTICAL STEPS IN DEVELOPING REVERSE-PHASE PEPTIDE SEPARATIONS

PRINCIPLES AND PRACTICAL STEPS IN DEVELOPING REVERSE-PHASE PEPTIDE SEPARATIONS

Learn how column chemistry and mobile phase affects peptide separation and selectivity. Review the factors that synergistically work to obtain resolution.

DEVELOPING REVERSE-PHASE PEPTIDE SEPARATIONS

Involved in developing oligonucleotide therapeutics? Oligonucleotides have attracted increasing attention in the development of direct therapeutic agents against a wide range of disease conditions.
The analysis of oligonucleotide therapeutics requires robust and accurate analytical characterization to confirm identity, and to determine purity, quality, and strength.
<h5>During this webinar, Thermo Scientific’s Ken Cook will examine developments and tips for:</h5>
<ul>
<li>Column chemistry to fully sequence a 100mer by LCMS/MS and HCD fragmentation
</li>
<li>A method to monitor IVT reactions by rapid UHPLC
</li>
<li>Observe disterio-isomer profiles in siRNA and ASO RNA
</li>
<li>Change separation selectivity in ion exchange and ion pair reverse phase chromatography of oligonucleotides with eluent choice and oligonucleotides with pH
</li>
<li>Methods for strand ratio determination with double stranded oligonucleotides
</li>
</ul>
Thermo Scientific has over 20 years of innovations and publications around the separation of oligonucleotides, please join our webinar to learn how the DNAPac family of Ion Exchange and Reversed Phase columns can optimize and enhance your nucleic acid separations. The session will finish with a short Q&amp;A and include a live chat with an expert.
<blockquote>
Presenter: Dr. Ken Cook
</blockquote>
<blockquote>
Dr. Ken Cook began his career in academia as a Biochemistry lecturer focused on protein chemistry and metabolism. Today, with well over 30 years’ industry experience working for Dionex, then Thermo Fisher Scientific, his vast knowledge supporting and developing solutions within chromatography brand him a bio-separations expert. 
</blockquote>
<blockquote>
Currently, his work involves the support of Bio-Pharmaceutical applications to characterise protein and oligonucleotide-based bio-therapeutics. This involves ongoing collaborations with the Biopharmaceutical industry and academia. Methods for the characterisation of therapeutic proteins include peptide mapping by LC/HRMS. Developments in charged variant analysis including novel coupling to MS, Glycan analysis, Aggregate analysis and on-line process monitoring. Gene therapy characterisation is a growing interest which has had much success. 
</blockquote>
<blockquote>
Given his immense experience, it is an honour to have Ken share his knowledge.
</blockquote>

Avantor: New Developments and Tips for Oligonucleotide Analyses

New Developments and Tips for Oligonucleotide Analyses

Topics: Column chemistry to fully sequence a 100mer by LCMS/MS and HCD fragmentation; A method to monitor IVT reactions by rapid UHPLC...

Superficially porous particles (SPPs) have advantages over traditional fully porous particles (FPPs) when packed into columns for chromatographic separation of biomolecules. Due to the presence of a solid silica core, analytes have a smaller distance to travel within a particle, thus minimizing diffusion due to mass transfer effects. Combined with a large pore diameter and a thin, porous shell, these particles can yield highly efficient peaks even for large proteins such as monoclonal antibodies.
In this webinar, you will learn about the advantages of using SPP-packed BIOshell™ columns for various large molecule separation challenges. Aspects of peptide mapping, intact analysis and middle-up analysis will be reviewed, and strategies for improving these assays will be provided. In addition, a unique 1.5 mm I.D. column geometry and its advantages in improving sensitivity and making methods more sustainable will be reviewed. Finally, strategies for characterizing oligonucleotides will be discussed in relation to how BIOshell™ can assist in analyzing this class of biomolecule.
Key learning objectives
<ul>
<li>Discover the importance of SPP particle architecture (pore size and shell thickness) and how it contributes to high-efficiency separations of biomolecules
</li>
<li>Explore how column chemistry can play a role in improving peptide mapping accuracy
</li>
<li>Learn how extra column dispersion can affect chromatographic performance and discover strategies to mitigate it
</li>
</ul>
Who should attend?
<ul>
<li>R&amp;D scientists/method developers (from Pharma, Clinical, University)
</li>
<li>R&amp;D supervisors/managers (from Pharma, Clinical, University)
</li>
<li>QC scientists (from Pharma, Clinical, University)
</li>
<li>QC managers (from Pharma, Clinical, University)
</li>
</ul>
<blockquote>
Presenter: Cory E. Muraco (MilliporeSigma)
</blockquote>
<blockquote>
Cory Muraco is the Biomolecule Workflows Product Manager at MilliporeSigma, Bellefonte, USA. Muraco joined the company in 2013, starting in the Chemical Standards division, developing and validating analytical methods for testing the purity of certified reference materials using GC and LC methods. In 2022, Muraco started his current role, where he is tasked with supporting the design, development, and execution of the marketing and R&amp;D strategies around MilliporeSigma’s bioanalytical strategy.
</blockquote>
<blockquote>
Presenter: Jemima Arnold (Editorial Team, SelectScience)
</blockquote>
<blockquote>
Jemima studied Cellular and Molecular Medicine at the University of Bristol, UK, and as a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
</blockquote>

Merck: Breaking the Boundaries of Biomolecule Separation with BIOshell™ UHPLC Columns

Breaking the Boundaries of Biomolecule Separation with BIOshell™ UHPLC Columns

Advantages of using SPP-packed BIOshell™ columns for various large molecule separation challenges.

Biomolecule Separation with BIOshell UHPLC Columns

Nitrosamines have been compounds of interest to the pharmaceutical industry in recent years due to the mutagenic nature of these impurities. N-Nitrosodimethylamine (NDMA) was the first of these impurities to be highlighted as hazardous to human health. Eventually, the list expanded to many nitrosamines in various drug substances and drug products.
More recently, nitroso drug substance-related impurities (NDSRI) became a major concern for the industry. As per regulations, any possibility for formation of nitroso impurities during synthesis or the manufacturing process of the drug substance must be evaluated, and steps must be taken to identify, quantify, and minimize or eliminate such impurities. Therefore, analytical laboratories require robust methods for detection and quantitation of these impurities.
By attending this webinar session, you will understand:
<ul>
<li>What nitrosamines are and why they have been discussed in recent years</li>
<li>Which parameters are critical in nitrosamine analysis in pharmaceutical drugs</li>
<li>What nitroso drug substance-related impurities (NDSRI) are and why it is critical to evaluate and eliminate these impurities</li>
<li>Why selectivity is too critical in nitrosamine analysis</li>
<li>Why the presence of alternative chemistries is crucial in handling current and upcoming nitrosamine challenges</li>
<li>The impact of column dimensions in achieving optimal chromatography for nitrosamines</li>
<li>How different Agilent LC chemistries help in developing robust methods for nitrosamines</li>
<li>The best practices for nitrosamine analysis</li>
</ul>
<blockquote>
Presenter: Manu Grover (HPLC Columns Business Development Manager, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Postgraduate in Pharmacy from Manipal Academy of Higher Education, India
<ul>
<li>Have more than 21 years of chromatography experience</li>
<li>Have worked in field of Manufacturing and Analytical Research to support IND, NDA and ANDA filings in pharmaceutical companies like Dr. Reddy&#39;s, &gt; &gt; - Panacea Biotec &amp; Ozone Pharmaceuticals.</li>
</ul>
</blockquote>
<blockquote>
During the Professional journey have:
<ul>
<li>Developed and validated many analytical methods on HPLC, GC, Particle sizing, DSC, pXRD, LC/MS etc.</li>
<li>Successfully filed multiple analytical Dossiers in US and EU market.</li>
<li>Filed analytical methods to USP</li>
<li>Transferred analytical methods</li>
<li>Handled multiple US-FDA audits</li>
<li>Trained many scientists on “Data integrity and compliance” during sessions organized by Pharmexcil-Govt. of India</li>
</ul>
</blockquote>
<blockquote>
The current role with Agilent sales team and pharmaceutical scientists share market updates, trends, expertise related to Agilent portfolio of columns and supplies. Also, ensure effective implementation of Agilent solutions in the lab.
</blockquote>
<blockquote>
Presenter: Chander Mani (Field Technical Specialist-LC Columns, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
After completing M.Sc. (Hons.) in Chemistry at Punjab University, Mr. Chander Mani started his career at Panacea Biotec in Analytical &amp; Bioanalytical department of new drug discovery research and developed several methods for analytical and bioanalytical, and ADME/DMPK related studies. He also worked for several LC-MS based methods for the analysis of textiles and environmental analytes of interests during his stay in Bureau Veritas. For the past more than 10 years at Agilent, Chander has been actively involved in providing LC-MS based application support to pharmaceuticals, food safety and environmental based applications in his various roles. Recently he has worked extensively for Nitrosamine application in various pharmaceuticals using both LCTQ and LCQTOF platforms and published various application notes.
</blockquote>

Agilent Technologies: Nitrosamines: Why Is Column Chemistry Critical?

Nitrosamines: Why Is Column Chemistry Critical?

Nitrosamines have been compounds of interest to the pharmaceutical industry in recent years due to the mutagenic nature of these impurities. 

Key Learning Points:
Discover the methodology for effectively evaluating and screening the most suitable HPLC column
Acquire comprehensive knowledge regarding the critical parameters crucial for column selectivity and evaluating a HPLC phase
This presentation will include a parallel comparison of Core-shell and fully porous columns
Who Should Attend:
<ul>
<li>Any HPLC, or Clinical users who are interested in learning tips and tricks in order to pick the right column</li>
</ul>
<blockquote>
Presenter: Shahana Huq (Senior Application Scientist, Phenomenex)
</blockquote>
<blockquote>
Shahana W. Huq is a Senior Application Scientist focusing on emerging technologies and solutions within Clinical Research and Sample Preparation, at the Phenomenex Applications Laboratory since the year 2000. She holds an MS in Biochemistry from California State University, Los Angeles. Her work involves experimental design, analytical method development, optimization, and troubleshooting. Furthermore, she has extensive expertise in R&amp;D product innovation and application development, as well as hands-on experience in sample preparation and LC-MS/MS. Shahana’s drive, and vision of the clinical field has motivated her to participate in various scientific publications and even as a coauthor of Phenomenex patent on sample preparation products (2006). Currently, Shahana works closely with global customers in the clinical research industry, providing technical support and consultation services as well as onsite seminars and webinars to promote peer development.
</blockquote>

Phenomonex: Column Selectivity Screening for Vitamin K Compounds

Column Selectivity Screening for Vitamin K Compounds

Methodology for effectively evaluating and screening the most suitable HPLC column.

For therapeutic antibodies and emerging gene-based medicines, light scattering techniques provide multi-attribute quantification (MAQ) and hence play a critical role in product and process development as well as quality control.
Size-exclusion chromatography with multi-angle light scattering (SEC-MALS) is employed throughout the development cycle of the protein therapeutics for bioanalytical characterization.
In addition, SEC-MALS offers a platform method for quantifying multiple quality attributes of AAV-delivered gene therapy products including titer, payload and aggregate content as well as extended biophysical characterization. In the webinar, we share some case studies using XBridge Premier BEH SEC columns combined with light scattering as a platform approach applied to these different pharmaceutical analytes.
<blockquote>
Presenter: Leo Liu, Ph.D. (Application Scientist, Waters-Wyatt Technology)
</blockquote>

Waters Corporation: Successful SEC-MALS analysis of proteins and AAVs

Successful SEC-MALS analysis of proteins and AAVs

Case studies using XBridge Premier BEH SEC columns combined with light scattering as a platform approach applied to these different pharmaceutical analytes.

LCMS webinars focusing on Columns - page 15

Column Selectivity Screening for Vitamin K Compounds

Nitrosamines: Why Is Column Chemistry Critical?

Successful SEC-MALS analysis of proteins and AAVs

Breaking the Boundaries of Biomolecule Separation with BIOshell™ UHPLC Columns

New Developments and Tips for Oligonucleotide Analyses

PRINCIPLES AND PRACTICAL STEPS IN DEVELOPING REVERSE-PHASE PEPTIDE SEPARATIONS

Chiral Chromatography Screening 101

Scale Up with Confidence: Column Selection for Preparative HPLC

Chiral Chromatography Screening 101

Strategies for Adding Xylazine, Nitazenes, and Other NPS to Laboratory Testing Scopes

Related content

News from LabRulezLCMS Library - Week 20, 2026

A comprehensive guide for selecting the appropriate HPLC detector

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells