<div><div><div>Scientists at IOCB Prague have developed a breakthrough technology that streamlines aptamer development. The new method integrates several biochemical and bioinformatics steps and paves the way for new diagnostic and therapeutic applications. Ongoing applications include wearable sensors that monitor selected biomarkers in real time and provide early warnings of impending health complications. The findings were published in Nature Communications.</div></div><div>Aptamers are short DNA or RNA strands that can recognize and bind to a specific target molecule with high precision. Similar to antibodies, they can be used to detect these molecules or modulate their activity. Unlike antibodies, they are much more stable, can be produced synthetically, and can be chemically modified to achieve the desired properties. As a result, they can offer capabilities that cannot be achieved with antibodies.</div><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg" alt="IOCHB Prague: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Chemically modified DNA aptamer bound to the target protein." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg 228w," sizes="100vw" width="1343" height="916">As demand grows for accurate and rapid diagnostic tools, aptamers are often better suited to these applications than antibodies. However, developing aptamers is both experimentally demanding and time-consuming. A team of scientists from IOCB Prague, led by Dr. Marek Ondruš and <a target="_blank" rel="noopener noreferrer" href="https://hocek.group.uochb.cz/en">Prof. Michal Hocek</a>, has now developed a technology that significantly shortens the development process.“Developing an aptamer is often like looking for a needle in a haystack. Among trillions of different DNA sequences, we search for the one that binds strongly and specifically to a target molecule. Our new approach first screen for ‘aptamer families’ – hundreds of related sequences – and then identify the best-performing member of the family. This approach can reduce development time from several months to only a few days,” says Marek Ondruš.<blockquote>Video:<a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=_3zXUeVMugU"> New technology streamlines the development of aptamers, synthetic alternatives to antibodies&nbsp;</a></blockquote>The team has also expanded the potential applications of aptamers by chemically modifying their building blocks. For example, introducing functional groups similar to those found naturally in amino acids allows the resulting molecules to combine the advantages of both DNA and antibodies: Their sequence is encoded by the order of nucleotides in DNA, while the attached chemical modifications greatly expand their ability to recognize biologically relevant targets and mimic interactions between proteins that are underlying many biological processes.The researchers demonstrated the potential of this new method using the human insulin receptor, a key protein that regulates blood sugar levels. They developed an aptamer that specifically binds to the receptor and elucidated the molecular basis of this interaction using cryo-electron microscopy. “We have shown that chemically introduced functional groups play a crucial role not only in target recognition and formation of the aptamer–protein complex, but also in stabilizing the DNA aptamer’s structure. Our findings provide new insights into how chemically modified nucleic acids can mimic key properties of proteins,” says Marek Ondruš.In the future, the technology could complement – or, in some applications, even replace – antibody-based diagnostic tools. Researchers are collaborating with <a target="_blank" rel="noopener noreferrer" href="http://iocbtech.cz/">IOCB Tech</a> on its further development, as the company helps to bring discoveries from IOCB Prague into practical applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg" alt="IOCHB Prague/Tomáš Belloň: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Dr. Marek Ondruš, first author of the study, and Prof. Michal Hocek, head of the Bioorganic and Medicinal Chemistry of Nucleic Acids research group at IOCB Prague." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg 234w," sizes="100vw" width="2000" height="1335"><blockquote><h4>Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-73676-y#citeas">Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries</a></h4>ablo Alberto Franco-Urquijo, Marek Ondruš, Jaroslav Kurfürst, Jana Škerlová, Irena Selicharová, Lucie Mužíková Čechová, Hana Šváchová, Alena Semerádtová, Anatolij Filimoněnko, Adéla Fejfarová, Jiří Homola, Tomáš Kouba &amp; Michal HocekNat. Commun., 2026<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41467-026-73676-y">https://doi.org/10.1038/s41467-026-73676-y</a>&nbsp;<h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Discovery of functional nucleic acids from randomized libraries typically relies on multiple, time-consuming iterative rounds of in vitro selection with low success rate. Here, we present a single-round selection strategy for rapid screening of multiple over-represented nucleobase-modified DNA libraries and various selection conditions, capable of identifying high-affinity modified aptamers. Double partition followed by amplification of eluted sequences, NGS analysis and clustering provides fast identification of aptamer candidates. Screening of modified DNA libraries containing modified adenine and uracil nucleotides bearing hydrophobic aromatic phenyl and indole moieties results in development of an aptamer binding human insulin receptor with sub-nanomolar affinity and exquisite specificity. Cryo-EM structure reveals the importance of each aromatic modification, either in stabilizing the secondary structure or facilitating interactions with the protein surface. This approach addresses the main drawbacks of aptamer selection and has potential for high-throughput screening and accelerating the development of next-generation aptamers for diagnostics or therapeutics.</div>

New technology streamlines the development of aptamers, synthetic alternatives to antibodies

Clarity 10.2 expands instrument control capabilities with new control modules and updates the MS extension to help you work more efficiently.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity</a> 10.2 highlights</h4><ul><li>New and updated control modules</li><li>Improved MS data import and export</li><li>Obsolete hardware support ended</li></ul><h4>Instrument control modules</h4>Clarity 10.2 introduces new instrument control modules from Annuo (AS-3117, FAS-3823) and updated instrument controls form Sykam and Ecom.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Instrument_control_modules_5de26782b0.jpg" alt="DataApex: Instrument control modules" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Instrument_control_modules_5de26782b0.jpg 228w," sizes="100vw" width="380" height="260"><h4>MS data import/export improvements</h4>Clarity improves MS data exchange, especially for *.cdf files. MS instruments now support export and import of multiple spectral data and non-spectral signal data in the same CDF file. Import also better handles uniform sampling information, m/z Step values, scale factors, and file names with non-ASCII characters. Exporting individual chromatogram signals from MS instruments is now available through a separate Export Chromatogram dialog.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg" alt="DataApex: MS data import-export improvements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg 228w," sizes="100vw" width="378" height="259"><h4>Obsolete hardware support terminated</h4>Due to changes in Windows driver security requirements, this update discontinues support for obsolete hardware, including INT7, INT9, U-PAD, U-PAD2, and CB20.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg" alt="DataApex: Obsolete hardware support terminated" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg 228w," sizes="100vw" width="378" height="259"><blockquote>Explore the complete list of all changes, fixes, and additions in version 10.2.👉<a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1">See the complete changelog&nbsp;</a></blockquote>

Clarity Chromatography Software 10.2 released!

🎤Dr. Leigh Terry (University of Alabama)In this episode of Concentrating on Chromatography, we sit down with Dr. Leigh Terry from the University of Alabama to explore one of the biggest challenges in environmental analysis today: understanding—and accurately measuring—PFAS in water systems.While most regulatory methods focus on a small number of compounds like PFOA and PFOS, there may be thousands of PFAS compounds in the environment—and much of that burden goes undetected.<h4>In this episode, we cover:</h4><ul><li>Why PFAS are called “forever chemicals” (and why that label is evolving)</li><li>The limitations of targeted LC-MS/MS methods</li><li>What the fluorine mass balance problem reveals about PFAS destruction</li><li>How combustion ion chromatography (CIC) helps measure total organofluorine</li><li>Why “99.99% removal” doesn’t always mean what you think</li><li>Where PFAS actually end up in wastewater treatment systems</li><li>The most effective technologies for PFAS removal today</li><li>Why drinking water may only account for ~20% of total PFAS exposure</li></ul><h4>Key Insight:</h4>Even when analyzing dozens of PFAS compounds, researchers may only be capturing a small fraction of total organic fluorine—raising major questions about how we monitor and regulate these contaminants.<h3>Video Transcription</h3>In this episode of Concentrating on Chromatography, Dr. Leigh Terry discusses PFAS contamination in drinking water, the analytical challenges associated with measuring these compounds, current treatment technologies, and future directions for monitoring and regulation. The conversation highlights how analytical chemistry, environmental engineering, and public health intersect in addressing one of today's most discussed classes of emerging contaminants.<h4>Understanding PFAS and Their Significance</h4><h5>The University of Alabama Water Quality Research Center</h5>Dr. Leigh Terry is part of the University of Alabama's Center for Water Quality Research. The center operates an analytical laboratory focused on drinking water quality and the detection of contaminants at extremely low concentrations.The laboratory specializes in:<ul><li>Drinking water analysis</li><li>Organic contaminants</li><li>Emerging contaminants</li><li>PFAS monitoring</li><li>Water quality research</li></ul>Their work supports both research initiatives and practical solutions for water utilities and environmental agencies.<h4>Why Focus on PFAS?</h4>Although PFAS compounds have existed since the 1940s, public awareness has increased dramatically only in recent years.According to Dr. Terry, several factors contributed to the rapid growth of PFAS research:<ul><li>Increased media attention</li><li>New regulatory initiatives</li><li>Growing evidence of human exposure</li><li>Expanding research opportunities</li><li>Greater understanding of potential health risks</li></ul>PFAS are not new contaminants. Rather, society is only beginning to understand their prevalence in the environment and their potential impacts on human health.<h4>The Development of Standardized PFAS Methods</h4>One major advancement in PFAS analysis has been the development of standardized EPA methods.These methods allow:<ul><li>Consistent testing procedures</li><li>Comparable results between laboratories</li><li>Regulatory monitoring</li><li>Reliable data interpretation</li></ul>Dr. Terry notes that many of these standardized approaches began appearing around 2018–2019, providing a common framework for analytical laboratories and researchers.<h4>How Large Is the PFAS Universe?</h4>Many people are familiar with PFOA and PFOS, two of the most heavily studied PFAS compounds. However, these represent only a small fraction of the broader PFAS family.PFOA and PFOS are often referred to as:<ul><li>Legacy PFAS compounds</li><li>Original PFAS contaminants</li><li>Early commercial PFAS chemicals</li></ul>The challenge is that even minor modifications to molecular structure create entirely new PFAS compounds.Researchers estimate:<ul><li>Thousands of PFAS compounds exist</li><li>Some estimates exceed 10,000 compounds</li><li>Exact numbers depend on how PFAS are defined</li></ul>Scientific debate continues regarding classification and definition, making precise counting difficult.<h4>Why PFAS Are Called "Forever Chemicals"</h4>Dr. Terry notes that the phrase "forever chemicals" has become widely used, although she suggests the terminology could benefit from rebranding.PFAS persistence results from two key chemical properties.<h5>1. Amphiphilic Structure</h5>PFAS molecules contain:<ul><li>A water-loving (hydrophilic) head group</li><li>A water-repelling (hydrophobic) fluorinated tail</li></ul>This unusual combination allows PFAS to partition between:<ul><li>Water</li><li>Solids</li><li>Air-water interfaces</li><li>Foam</li></ul>Unlike many organic compounds, PFAS can interact effectively with multiple environmental phases.<h5>2. Extremely Strong Carbon–Fluorine Bonds</h5>The carbon–fluorine bond is among the strongest bonds in organic chemistry.As Dr. Terry explains:<ul><li>Large amounts of energy are required to break these bonds</li><li>Natural degradation processes are often ineffective</li><li>Destruction can require highly aggressive treatment conditions</li></ul>These properties make PFAS extremely persistent in both environmental systems and biological organisms.<h4>Toxicity, Regulations, and Environmental Behavior</h4><h5>Understanding PFAS Toxicity</h5>One of the biggest challenges in PFAS regulation is the incomplete understanding of toxicity.Dr. Terry explains that EPA regulations generally consider three factors:<ul><li>Presence in drinking water sources</li><li>Toxicity to humans</li><li>Availability of practical treatment options</li></ul>While some PFAS compounds are known to be harmful, many others remain insufficiently studied. Furthermore, PFAS often occur as mixtures rather than individual compounds.This creates important scientific questions:<ul><li>What is the toxicity of individual PFAS?</li><li>What is the toxicity of PFAS mixtures?</li><li>How should cumulative exposure be evaluated?</li></ul>These uncertainties continue to drive active research.<h4>What Happens to PFAS in Wastewater Treatment Plants?</h4>PFAS do not behave like many conventional contaminants.Rather than remaining fully dissolved in water, they often accumulate at interfaces such as:<ul><li>Water-solid interfaces</li><li>Water-air interfaces</li><li>Foam layers</li><li>Biological solids</li></ul>As a result:<ul><li>Some PFAS accumulate in biosolids</li><li>Some partition into foam</li><li>Some remain in treated effluent</li></ul>Their distribution depends on wastewater composition and treatment processes.<h4>Regulatory Monitoring and Targeted LC-MS Analysis</h4>Current EPA regulations focus on targeted analysis of specific PFAS compounds.Examples include:<ul><li>PFOA</li><li>PFOS</li></ul>Drinking water regulations currently establish maximum contaminant levels (MCLs) for these compounds at extremely low concentrations.Advantages of targeted LC-MS analysis include:<ul><li>Clearly defined analytes</li><li>Established regulatory limits</li><li>High analytical sensitivity</li><li>Reliable quantitation</li></ul>However, limitations remain:<ul><li>Only a small subset of PFAS is measured</li><li>Many PFAS compounds remain undetected</li><li>Total PFAS burden may be underestimated</li></ul>As Dr. Terry notes, targeted analysis provides only part of the overall picture.<h4>The Fluorine Mass Balance Problem</h4>One of the most important concepts discussed in the interview is fluorine mass balance.When PFAS degrade, fluorinated fragments may remain in the environment.This creates a critical question:Are treatment technologies truly destroying PFAS, or merely transforming them into shorter-chain PFAS compounds?To answer this, researchers perform fluorine mass balance calculations using:<ul><li>Targeted PFAS analysis</li><li>Fluoride measurements</li><li>Total organic fluorine measurements</li></ul>A successful destruction process should ultimately convert PFAS into fluoride ions rather than simply producing smaller PFAS molecules.<h4>Advanced Analysis, Treatment Technologies, and Future Directions</h4><h5>Combining Targeted and Bulk Fluorine Measurements</h5>Dr. Terry's group complements targeted LC-MS analysis with measurements of total organic fluorine.Approaches include:<ul><li>Absorbable Organic Fluorine (AOF)</li><li>Extractable Organic Fluorine (EOF)</li><li>Combustion Ion Chromatography (CIC)</li></ul>In these methods:<ul><li>Organic fluorine is captured</li><li>Samples are combusted</li><li>Released fluoride is quantified</li></ul>This provides insight into total fluorinated content, although contributions may originate from sources beyond PFAS, including pharmaceuticals and consumer products.<h4>Surprising Findings from Combined Analysis</h4>One important finding from wastewater studies was that targeted PFAS measurements accounted for only a small fraction of total organic fluorine.Dr. Terry reports that:<ul><li>Approximately 4% of measured AOF could be explained by targeted PFAS compounds</li><li>The majority of organic fluorine remained unidentified</li></ul>This suggests that environmental fluorine chemistry is considerably more complex than currently represented by routine monitoring programs.<h4>Preventing PFAS Contamination in the Laboratory</h4>PFAS analysis requires exceptionally strict quality control.Dr. Terry's laboratory routinely:<ul><li>Tests every new batch of SPE cartridges</li><li>Tests carbon tubes before use</li><li>Uses DI water blanks</li><li>Verifies consumables labeled as PFAS-free</li><li>Collects field blanks</li><li>Monitors analytical blanks throughout workflows</li></ul>These practices help prevent contamination from laboratory materials and consumables, a common challenge in ultra-trace PFAS analysis.<h4>Current PFAS Treatment Technologies</h4>Three technologies currently dominate PFAS removal in drinking water treatment:<ul><li>Granular Activated Carbon (GAC)</li><li>Ion Exchange Resins</li><li>Reverse Osmosis (RO)</li></ul>Selection depends on:<ul><li>PFAS concentration</li><li>Water quality</li><li>Local operating conditions</li><li>Utility infrastructure</li></ul>Although many emerging technologies are being investigated, these remain the most widely implemented solutions.<h4>Membrane Filtration and Pretreatment</h4>Dr. Terry collaborates with colleagues studying membrane-based PFAS treatment.Her own work focuses on pretreatment through biological filtration.The strategy involves:<ul><li>Removing natural organic matter</li><li>Reducing membrane fouling</li><li>Improving membrane performance</li><li>Extending operational efficiency</li></ul>This integrated approach demonstrates how multiple treatment technologies can work together to improve PFAS removal.<h4>Regional Monitoring Projects</h4>The University of Alabama team participates in EPA-supported monitoring efforts along the Gulf Coast.Their observations indicate:<ul><li>PFAS rarely occur alone</li><li>Multiple PFAS compounds frequently co-occur</li><li>Contamination often originates from identifiable point sources</li></ul>Examples include:<ul><li>Industrial facilities</li><li>Manufacturing operations</li><li>Military installations</li><li>Carpet manufacturing facilities</li></ul>Source identification remains a critical component of environmental monitoring programs.<h4>Future Directions for PFAS Monitoring</h4>Looking ahead, Dr. Terry sees several opportunities for improvement.Desired advances include:<ul><li>In situ monitoring technologies</li><li>Portable detection systems</li><li>Reduced analytical complexity</li><li>Lower instrument costs</li><li>Less expensive consumables</li><li>Faster analysis workflows</li></ul>Current PFAS testing requires substantial expertise, specialized instrumentation, and rigorous quality control, making widespread implementation challenging.<h4>Dr. Terry's Perspective on Long-Term Solutions</h4>When asked what major PFAS challenge she would most like to solve, Dr. Terry offered a broader perspective.Rather than focusing solely on remediation, she emphasized prevention.Her view is that society should:<ul><li>Better evaluate chemical toxicity before commercialization</li><li>Assess risks before large-scale deployment</li><li>Reduce introduction of harmful compounds into the environment</li><li>Support utilities tasked with contaminant removal</li></ul>She notes that water utilities often bear the responsibility of removing contaminants they did not create, highlighting the need for broader societal and regulatory support.<h4>Final Thoughts</h4>Dr. Terry encourages consumers to:<ul><li>Learn where their drinking water originates</li><li>Read annual water quality reports</li><li>Stay informed about local water quality</li><li>Consider appropriate home filtration where needed</li></ul>At the same time, she emphasizes confidence in municipal water systems and the importance of continued investment in water quality research, monitoring, and treatment technologies.<h4>Conclusion</h4>PFAS remain one of the most complex challenges in environmental chemistry and water treatment. Through advanced analytical methods such as LC-MS/MS, combustion ion chromatography, fluorine mass balance approaches, and large-scale monitoring programs, researchers like Dr. Leigh Terry are helping improve our understanding of these persistent contaminants.The interview highlights that solving the PFAS challenge will require a combination of better analytical tools, improved treatment technologies, stronger regulatory frameworks, and a greater emphasis on prevention before contaminants enter the environment.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

PFAS in Water: Why We’re Missing 96% of the Problem

Article

Product

Video

Scientists

Environment

Water

Interview

Science and research

Software

Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

DataApex

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Metrohm

Agilent Technologies

Phenomenex

Thermo Fisher Scientific

SelectScience

VWR/Avantor

Waters Corporation

In this session, explore the operation and latest developments of automated SPME for VOCs and SVOCs by headspace, direct immersion, and derivatization techniques.
Furthermore, discover how SPME has key applications for food, water, environmental and forensic samples for use in laboratories for GC-MS and LC-MS and direct MS analysis.
<blockquote>
Presenter: Hans-Joachim Huebschmann (Consultant, VP Sample Preparation, CTC Analytics)
</blockquote>

SelectScience: Solid phase microextraction – How does it work for my samples?

Solid phase microextraction – How does it work for my samples?

Explore the operation and latest developments of automated SPME for VOCs and SVOCs.

Solid phase microextraction–How does it work for my samples?

Key Learning Points:
<ul>
<li>Understand how to obtain good resolution by utilizing selectivity
</li>
<li>Learn the basics of different stationary phase particle characteristics 
</li>
<li>Discover how to choose different stationary phases based on compound classes
</li>
<li>Find how mobile phase can be used to change selectivity of columns
</li>
</ul>
Who Should Attend:
<ul>
<li>Any LC users who are looking for are looking for a basic overview of Reverse Phase Method Development by looking at different small molecule compound classes</li>
</ul>
<blockquote>
Presenter: Genevieve Hodson (Senior Application Scientist, Phenomenex)
</blockquote>
<blockquote>
Genevieve graduated from the University of Texas with a BS in Chemistry located in her home town of Austin Texas. She spent 2 years of her undergraduate performing research in an Organic Chemistry Lab synthesizing intermediates. Out of school she spent 4 years as an Analytical Chemist in the QC and R&amp;D Department of Cerilliant (a Sigma Millipore Company). There she worked with many analytical instruments but became an expert at LC by performing purity analysis, customer specific methods, validations, qc testing and stability studies on a wide range of small molecules including pharmaceuticals, pesticides, explosives and illegal substances. From there she moved to Los Angeles California where she worked at Johnson and Johnson and then a small dietary supplement company. After Genevieve spent a year living abroad in Israel raising her first son, she moved back to LA where she got hired as a Technical Specialist at Phenomenex. At Phenomenex she enjoys continuing to learn about chromatography every day through helping customer with all their chromatography needs!
</blockquote>

Phenomenex: Understanding Reverse Phase Selectivity for Different Compound Classes

Understanding Reverse Phase Selectivity for Different Compound Classes

Understand how to obtain good resolution by utilizing selectivity.

Understanding Reverse Phase Selectivity

Having a list of things to consider before starting your HPLC analysis is good practice. It can help you to avoid chromatography problems down the line.
In this webinar, we will discuss:
<ul>
<li>Sample: Get to know your target analytes, interferences present in the sample matrix, and how to prepare the sample for HPLC analysis
</li>
<li>Supplies: The importance of using appropriate supplies to get the best results
</li>
<li>Column: How to select the column, equilibrate it, benchmark it with your system, and take care of it
</li>
<li>LC instrument: Getting to know your LC system, the importance of maintaining it, and having a “housekeeping” list for it
</li>
</ul>
<blockquote>
Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.
</blockquote>
<blockquote>
Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.
</blockquote>
<blockquote>
Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
</blockquote>

Agilent Technologies: What to Consider Before Starting Your HPLC Analysis

What to Consider Before Starting Your HPLC Analysis

The importance of using appropriate supplies to get the best results.

Solid-Phase Extraction (SPE) is a fundamental sample preparation technique widely used across all areas of analytical chemistry.
Whether new to SPE or seeking a refresher, this webinar will introduce the basics of sample preparation and provide practical insights and technical background on the benefits when choosing SPE over another form of sample preparation, especially for analytes in complex matrices. Join us in exploring SPE retention mechanisms, device format and sorbent selection, and best practices for implementing successful SPE methods. This webinar is designed for both beginners and experienced scientists looking to reinforce their understanding of this sample clean-up tool.
Learning Objectives Include:
<ul>
<li>Understanding SPE Fundamentals
</li>
<li>Optimizing SPE by Selecting an Appropriate Sorbent, Format, and Protocol
</li>
<li>Verifying Successful SPE Implementation and Troubleshooting Tips
</li>
</ul>
<blockquote>
Presenter: Chelsea Plummer (Senior Product Marketing Manager, Waters Corporation)
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<blockquote>
Chelsea Plummer, PhD is a Senior Product Marketing Manager with a background in bioanalytical mass spectrometry. Graduating from Purdue University and The University of Alabama, her research focused on analysis of small molecules and peptides by both quantitative and high resolution MS. At Waters, Chelsea has supported customers across product specialist roles in both consumables and mass spectrometry. Later moving to Waters’ product team, she now covers sample preparation, particularly focused on solid-phase extraction products for use in bioanalysis, food, and environmental applications. Chelsea enjoys working closely with the scientific team at Waters to provide customers with helpful sample preparation solutions.
</blockquote>

Waters Corporation: Solid-Phase Extraction: Back to Basics

Solid-Phase Extraction: Back to Basics

Basics of sample preparation and provide practical insights and technical background on the benefits when choosing SPE over another form of sample preparation.

In this on-demand webinar, Wai-Chi Man, Product Marketing Manager at Thermo Fisher Scientific, discussed the basics of why Ion Chromatography coupled with Mass Spectrometry (IC-MS) can be an added benefit to any High-Performance Liquid Chromatography with Mass Spectrometry (LC-MS), and complimentary to any Hydrophilic Interaction Liquid Chromatography (HILIC) analysis.
You can also expect to:
<ul>
<li>Enhance your understanding of the instrument requirements for IC and MS: which one is best for your application of interest.
</li>
<li>Learn how to set up the connectivity or simply switch from IC-MS to LC-MS.
</li>
<li>Explore the switch over from applications such as Polar Pesticides from Anionic to Cationic with one instrument set-up, and how it’s applicable to other applications such as Metabolomics.
</li>
</ul>
<blockquote>
Presenter: Wai-chi Man (Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Wai-Chi Man, MRSC has been with Thermo Fisher Scientific since 2011. She started on the application sales support team but is now a product marketing manager. She has specialized in ion chromatography (IC) for more than 33 years, with over 36 years of experience in analytical chemistry in a wide variety of techniques. Before Thermo Fisher Scientific, she spent seven years at Wellcome Research supporting structural elucidation for medicinal chemists using a wide range of analytical instrumentation. Wai-Chi spent a total of 20 years in the pharma industry as part of GlaxoSmith Kline, from research to transferring methods to manufacturing. She worked on and was responsible for many drugs from anti-viral to respiratory in the market today.
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<blockquote>
Wai-Chi’s work at Thermo Fisher Scientific has supported a wide range of customers from various industries, providing IC support in method development, troubleshooting, sample preparation, trainings, seminars, events, and workshops. This experience has helped her build greater knowledge, skill and understanding within areas such as IC-MS, IC-ICP-MS, HPAE-PAD analysis, metabolomics, and polar pesticides.
</blockquote>

Thermo Scientific: Back to Basics – IC-MS 

Back to Basics – IC-MS 

The basics of why Ion Chromatography coupled with Mass Spectrometry (IC-MS) can be an added benefit to any High-Performance Liquid Chromatography.

Mass Spectrometry Imaging (MSI) is a technique that allows chemical information to be obtained directly or via the use of matrices from a wide range of surfaces. MS Imaging provides information on the spatial distribution of molecules at or near the surface. Imaging provides beautiful and information-rich images that allow scientists to answer the fundamental question: “Where is this component in my sample?”
In this webinar series, we will examine the basics of MS imaging, compare the techniques of untargeted and targeted MS Imaging and the information that can be gained from each, and end with an interview from Dr. Ryan Leib (Stanford University) and Dr. Roy Martin (Waters Corporation) on the practicalities of setting up a MS Imaging Core lab.
Who Should Attend: Any scientist, lab managers or lab directors looking to expand (or refresh) their knowledge of MS Imaging.
<h3>Part Two – October 17th, 2023: Untargeted versus Targeted Imaging</h3>
In the second session, we will look at the differences between untargeted and targeted imaging: the systems on which the data can be acquired, what data is collected using each technique, how data can be analyzed and how they can work together to provide a complete picture.
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Presenter: Anthony Midley (Principal Applications Support Scientist, Waters Corporation)
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<blockquote>
Anthony Midey is a Principal Applications Support Scientist in Waters Biomedical Research group at Americas Application Laboratory, providing pre- and post-sales support in DESI, MALDI, and imaging mass spectrometry. He is also the BMR team lead for targeted metabolomics and lipidomics LC-MSMS applications. Anthony received his Ph.D. in spectroscopy and mass spectrometry from the University of Rochester and joined Waters in 2015. He has over 55 publications in peer-reviewed journals, with over 10 years working in the analytical instruments sector.
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<blockquote>
HOST: Kieron Faherty, Ph.D. (Separations and Mass Spectrometry Field Marketing Manager, Waters Corporation)
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<blockquote>
Kieron Faherty is the Separations and Mass Spectrometry Field Marketing Manager for the Americas at Waters Corporation. He received his B.Sc. (Hons) in Chemistry from the University of Reading (Reading, Berkshire, UK) and his doctorate in Chemistry from the University of Massachusetts (Amherst, MA). He has held several field-based positions at Waters, most recently as a Field Applications Specialist focused on biopharmaceutical applications using Time-of-Flight mass spectrometry instrumentation.
</blockquote>

Waters Corporation: Untargeted versus Targeted Imaging

Untargeted versus Targeted Imaging

We will look at the differences between untargeted and targeted MS imaging.

Mass Spectrometry Imaging (MSI) is a technique that allows chemical information to be obtained directly or via the use of matrices from a wide range of surfaces. MS Imaging provides information on the spatial distribution of molecules at or near the surface. Imaging provides beautiful and information-rich images that allow scientists to answer the fundamental question: “Where is this component in my sample?”
In this webinar series, we will examine the basics of MS imaging, compare the techniques of untargeted and targeted MS Imaging and the information that can be gained from each, and end with an interview from Dr. Ryan Leib (Stanford University) and Dr. Roy Martin (Waters Corporation) on the practicalities of setting up a MS Imaging Core lab.
Who Should Attend: Any scientist, lab managers or lab directors looking to expand (or refresh) their knowledge of MS Imaging.
<h3>Part One – October 10th, 2023: What is mass spectrometric imaging?</h3>
In this session, we will examine the types of MS imaging techniques on the market and the platforms on which they can be run. We will also discuss the types of data and information that MS imaging can provide and what imaging provides a user that other mass spectrometric techniques cannot.
<blockquote>
Presenter: Bindesh Shrestha (Senior Application Support Scientist, Waters Corporation)
</blockquote>
<blockquote>
Bindesh Shrestha, PhD., is an expert in the field of imaging mass spectrometry and ambient ionization. He completed his graduate work at the George Washington University and is currently employed with the Waters Corporation. In Waters, Bindesh supports academic grant efforts with biomedical applications, such as imaging MS and metabolomics. Bindesh has co-authored about three dozen peer-reviewed articles in leading journals, such as Angewandte Chemie, Analytical Chemistry, Analyst, Nature Methods. In the last two years, he edited a book on single-cell metabolism with Springer and published an introductory book on imaging mass spectrometry with Elsevier.
</blockquote>
<blockquote>
HOST: Kieron Faherty, Ph.D. (Separations and Mass Spectrometry Field Marketing Manager, Waters Corporation)
</blockquote>
<blockquote>
Kieron Faherty is the Separations and Mass Spectrometry Field Marketing Manager for the Americas at Waters Corporation. He received his B.Sc. (Hons) in Chemistry from the University of Reading (Reading, Berkshire, UK) and his doctorate in Chemistry from the University of Massachusetts (Amherst, MA). He has held several field-based positions at Waters, most recently as a Field Applications Specialist focused on biopharmaceutical applications using Time-of-Flight mass spectrometry instrumentation.
</blockquote>

Waters Corporation: What is Mass Spectrometry Imaging?

What is Mass Spectrometry Imaging?

We will examine the types of MS imaging techniques on the market and the platforms on which they can be run.

The pH value is one of the most important and therefore also one of the most frequently measured parameters in analytical chemistry.
Sometimes, a simple pH test strip will do for a quick check; however, a precise pH meter and the right choice of the pH electrode are a must when reliable results are needed. 
In our educational webinar on November 14, Doris Hoffmann, Product Manager at the Competence Center Titration at Metrohm International Headquarters, will show you that the determination of the pH value can be quite simple, if you pay attention to a few critical details. 
Whether you perform a pH measurement for the first time or are a routine user, we are sure there is room for improvement. Join this webinar and make the next step towards higher accuracy and better reproducibility of your pH measurement results.
Register &amp; participate in this webinar to learn:
<ul>
<li>What the pH value is and how it is measured
</li>
<li>The functional components and design of a pH electrode
</li>
<li>Which kind of pH electrode is suitable for which application
</li>
<li>What parameters are determined during a pH calibration, and what values are acceptable
</li>
<li>How to properly care for and maintain a pH electrode
</li>
</ul>
<blockquote>
Presenter: Doris Hoffmann (Product Manager at CC Titration, Metrohm International Headquarters)
</blockquote>
<blockquote>
Doris Hoffmann has studied chemistry and has specialized in analytical chemistry and electrochemistry in her Master`s degree. She joined Metrohm International Headquarters in 2021 as a Product Manager and is responsible for the extensive portfolio of Metrosensors including pH electrodes, electrodes for titration, and more.
</blockquote>

Metrohm: The basics of precise pH measurement

The basics of precise pH measurement

The determination of the pH value can be quite simple, if you pay attention to a few critical details.

Hydrophobic interaction chromatography (HIC) is a powerful method for analyzing and characterizing protein-based therapeutics under native conditions. It is the gold standard method for antibody-drug-conjugate (ADCs) analyses, but is equally suitable for the analysis of protein modifications such as oxidations, post-translational modifications or deamidations in protein therapeutics such as monoclonal antibodies (mAbs). This webinar will explain how HIC works, present application examples, and provide tips and tricks for method development.
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Presenter: Andrea Krumm (Product manager, Tosoh Bioscience GmbH)
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Andrea Krumm studied biotechnology before gaining a PhD in cell- and cancer-biology. After four years as an applications specialist for optical analytical devices used in academic and pharmaceutical research, she joined Tosoh Bioscience GmbH in 2020 as a product manager for analytical columns. As such she is responsible for gathering customer requirements, application areas and addressing these with new and existing products of the analytical columns line and Tosoh’s Multi-Angle-Light-Scattering Detector LenS3.
</blockquote>

VWR - Avantor: Hydrophobic interaction chromatography: a versatile method for ADC and protein analysis

Hydrophobic interaction chromatography: a versatile method for ADC and protein analysis

Hydrophobic interaction chromatography for ADC analysis

Polar analytes vary in complexity and chemical properties, ranging from nucleotides to small polar acids, like citric acid. It can be difficult to know where to begin when developing methods. Many options exist to retain these compounds. The differences between the two major techniques, reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC), are considerable. Each has its own benefits and drawbacks. Additionally, within each separation technique, specialized stationary phases can provide unique selectivity or retentivity benefits, specifically for polar retention.
This webinar will highlight the two most common techniques employed to retain polar analytes. A discussion of reversed-phase polar retention columns will demonstrate how low ligand density phases and mixed mode columns can be used successfully followed by a look at HILIC and the effective stationary phases for successful separations. In addition, information about how MaxPeak Premier High-Performance Surfaces (HPS) technology can be used to improve polar analyte analysis will be provided. By understanding the different techniques and considering the different stationary phases and technologies available, method developers can find more suitable analysis conditions for their tricky polar analytes.
<blockquote>
Presenter: Kenneth Berthelette (Senior Scientist, Waters Corporation Chemistry and Consumables Group)
Ken earned his Bachelor&#39;s of Science degree in Professional Chemistry from Bridgewater State University, Bridgewater Massachusetts, in 2010. He has been with Waters Corporation since March 2012 and has worked on several product launches including CORTECS columns, UPC2 Torus and Trefoil columns, and the Atlantis Premier BEH Z-HILIC column. He changed job functions in 2021 and now supports Chemistry Aftermarket initiatives. His focus on applications and customer education has led to several seminars and webinars on topics including method development, method modernization and transfer, HILIC separation techniques, and LC system troubleshooting.
</blockquote>

Waters Corporation: Reversed-Phase or HILIC? Optimizing Retention of Polar Analytes

LCMS webinars focusing on Academy/Principles - page 20