<div>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</div><div><div><div><div><h3>What is Protein Purification?</h3>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.This process is especially vital in biotechnological areas inside immunology and microbiology. In immunology, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In microbiology, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</div></div></div></div><div><div><div><div><h3>Types of Protein Purification Methods</h3></div></div></div></div><div>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</div><div><div><div><div><h4>Non-Chromatographic Approaches</h4></div></div><div>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.<h5>Precipitation Techniques</h5>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:<ul><li>Organic solvent precipitation involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li>Isoelectric point precipitation exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li>Nonionic polymer precipitation uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5>Dialysis</h5>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.<h5>Immunocapture (Magnetic Bead-Based Purification</h5>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:<ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.<h5>Centrifugation</h5>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.Preparative Ultracentrifugation Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.Differential Centrifugation Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.Rate-zonal Gradient Centrifugation This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.Isopycnic Centrifugation (Equilibrium Centrifugation) Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</div></div></div><div><div><div><div><h4>Chromatographic Approaches</h4>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.<h5>Ion Exchange Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526">Luna SCX </a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540">Biozen WCX</a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5>Size Exclusion Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.Phenomenex offers a range of Biozen columns for SEC separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534">Biozen dSEC-2</a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"> Biozen dSEC-7 </a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533">Biozen SEC-2</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532">Biozen SEC-3</a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.<h5>Reversed-Phase Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.<h5>Hydroxyapatite Chromatography</h5>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.<h5>Hydrophobic Interaction Chromatography</h5>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.<h5>Affinity Chromatography</h5>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

Article

Product

Application

Academy

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Separation Science

Metrohm

Mettler-Toledo

Technology Networks

SelectScience

Waters Corporation

By attending this eSeminar, you will:
<ul>
<li>Gain practical insights into optimizing sample preparation in alignment with EPA Method 1633’s regulatory requirements by the use of dual-phase SPE cartridges, covering background analysis, MDL studies, and recoveries</li>
<li>Benefit from real-world experiences from Complete Environmental Testing, offering valuable insights on navigating EPA 1633 in an end-to-end workflow, while utilizing dual-phase SPE cartridges to improve laboratory efficiency</li>
<li>Understand key details for successful PFAS analysis</li>
<li>Learn how to optimize analysis for ultra-short chain PFAS compounds</li>
<li>See how to expand the range of PFAS compounds to analyze in a single run</li>
</ul>
<h4>Event Overview</h4>
Separation Science, in collaboration with its sponsors Waters and Restek, is pleased to bring you an upcoming online eSeminar focusing on advances in PFAS analysis. This unique event will bring together key experts in the field and provide an interactive learning environment for scientists working in this industry.
<h4>Presentations</h4>
Efficiency in EPA Method 1633: Validation and Implementation Strategies
by Jeffrey Smith, Director of Operations, Complete Environmental Testing (CET)
Join us for a presentation focused on optimizing EPA Method 1633 in your laboratory highlighting the validation and implementation of dual-phase WAX and GCB cartridges. EPA 1633 establishes a method for the determination of 40 PFAS from challenging matrices such as non-potable waters, soils, biosolids, and tissue. For these matrix types, sample preparation has moved beyond the use of a WAX cartridge alone. GCB has been added to the sample preparation workflow to provide additional sample cleanup. We will discuss the full EPA 1633 workflow, including topics such as background analysis for soils and water, MDLs achieved, observed recovery in authentic samples, and the practical advantages of dual-phase cartridges in reducing manual labor steps compared to loose GCB in EPA 1633.
This presentation is designed for scientists, laboratory managers, and others looking to implement and validate EPA Method 1633 or optimize their sample preparation process in their current workflow. Join us as Complete Environmental Testing shares experiences on working with the complex matrices in EPA 1633 and breaks down advantages in their method workflow with the use of dual-phase SPE cartridges.
Untapped Ultrashort-chain PFAS Analysis Combined with Routine PFAS Analysis in a Single LC-MS/MS Run
by Mike Chang, Market Development Manager, Restek Corporation
Starting from measuring C8 many decades ago, today’s PFAS testing requires a lot more chromatography capabilities when an extended list of PFAS analysis is desired. Chain length ranging from C1 to C18 and a functional group changing from carboxylic acids to ethers, sulfonamides, and more, with multiple oxygens in the structure, can be challenging for a chromatograph all in one run. In this presentation, we will see examples of a combined PFAS panel analyzed in one run with chain length from C1 and above, in addition to AFFF and FTS compounds.
<h4>What you need to know:</h4>
Date: 16 April, 2024
Start times:
<ul>
<li>Broadcast 1: 9 am BST (London) / 10 am CEST (Paris/Berlin) / 1:30 pm IST (New Delhi) / 4 pm SGT (Singapore)
</li>
<li>Broadcast 2: 10 am PDT (Los Angeles) / 12 pm CDT (Chicago) / 1 pm EDT (New York)
</li>
</ul>
Duration: Approximately 90 minutes
<blockquote>
Presenter: Jeffrey Smith (Director of Operations, Complete Environmental Testing (CET))
</blockquote>
<blockquote>
Jeffrey Smith holds a BS in Chemistry from Quinnipiac University. He has been working in environmental analytical chemistry for 18 years, with experience in several disciplines, including GC/MS and LC-MS/MS. Jeffrey now serves as the director of operations at Complete Environmental Testing. For the past few years, his focus as director has been building the PFAS department at CET from the ground up, including method development and researching LC/MS/MS instrumentation for the laboratory. Jeffrey serves on several PFAS work groups and is an active member of the Reasonable Confidence QA/QC Protocols work group for the state of Connecticut.
</blockquote>
<blockquote>
Presenter: Mike Chang (Market Development Manager, Restek Corporation)
</blockquote>
<blockquote>
With an analytical chemistry background and 15+ years of experience in the chromatography industry, from instrumentation to applications, Mike is the key liaison to testing agencies and industries, bringing better ways of measuring contaminants in the environment and food. He has been chairing PFAS sessions and presenting PFAS topics at various conferences.
</blockquote>

Advances in PFAS Analysis

Understand essential details for successful PFAS analysis and optimize EPA Method 1633 in your lab.

Liquid handling is one of the most important yet also tedious tasks in the analytical laboratory. Only the correct volume of sample, solvent, reagent, titrant, or auxiliary solution makes sure analytical results are accurate and reproducible.
In this webinar, Jennifer Lüber, Product Specialist Liquid Handling and Lab Robotics at Metrohm International Headquarters, will give you an introduction to the automation of liquid handling. After a brief overview of the capabilities of various dosing systems, Jennifer will demonstrate how the automation of basic liquid handling tasks will help you improve the quality of your analytical results while freeing up valuable time for other tasks. From this, a path forward can emerge to an automation solution that supports your analytical workflow best.
During a Q&amp;A session at the end of her webinar you can ask Jennifer any questions you may have about liquid handling and the solutions from Metrohm. Join us on April 17 and get inspired by what’s possible when it comes to the automation of your liquid handling!
Key learnings of this webinar:
<ul>
<li>Better results, less risk of errors – the benefits of automated liquid handling</li>
<li>When to automate and what: How to find the best automation solution to support your analytical workflow</li>
<li>Tips and tricks how to keep your system at peak performance</li>
</ul>
Who should attend?
Lab technicians, chemists, and everybody else performing liquid handling tasks in the laboratory.
<blockquote>
Presenter: Jennifer Lüber (Product Specialist for Liquid Handling and Lab Robotics, Metrohm International Headquarters)
</blockquote>
<blockquote>
Jennifer Lüber is currently the Product Specialist for Liquid Handling and Lab Robotics at Metrohm International Headquarters in Switzerland. She finished her apprenticeship as a Lab Technician in 2014 and joined the Competence Center Titration at Metrohm International Headquarters in 2015. In 2020 Jennifer received her bachelor degree in business process management and in 2023, she joined the Competence Center Liquid Handling and Lab Robotics to support the team with her experience.
</blockquote>

Metrohm: Automated liquid handling – the key to accurate and reproducible results

Automated liquid handling – the key to accurate and reproducible results

In this webinar, we will give you an introduction to the automation of liquid handling.

Automated liquid handling

Key Learning Points:
<ul>
<li>Determine the best SPE sorbent and starting conditions for your analytes in a single run</li>
<li>Conserve valuable lab resources by spending less time developing SPE methods</li>
<li>Learn how to quickly identify SPE conditions for multi-analyte panels</li>
</ul>
<blockquote>
Presenter: Stephanie J. Marin (Clinical and Forensics Global Market Development Manager at Phenomenex)
</blockquote>
<blockquote>
She received her Ph.D. in chemistry from Arizona State University, and has expertise in sample preparation, liquid chromatography and mass spectrometry. She has over 10 years of experience developing and validating clinical methods from her tenure at the ARUP Institute for Clinical and Experimental Pathology. Dr Marin has worked in marketing, product development, and applications development, in addition to customer facing roles in customer and technical support and service and customer education and training. She has also held positions at Hamilton Company, Selerity Technologies and Biotage, and in analytical services for specialty chemicals and polymers at Rohm and Haas (now Dow). She was also a supervisor at an EPA certified laboratory. She is the author of over 30 peer reviewed publications and book chapters and over 100 abstracts presented at national and international meetings. 
</blockquote>

Phenomenex: Effortless Solid Phase Extraction (SPE) Method Development in a Single Experiment

Effortless Solid Phase Extraction (SPE) Method Development in a Single Experiment

Determine the best SPE sorbent and starting conditions for your analytes in a single run.

Solid Phase Extraction (SPE) Method Development

At the forefront of scientific inquiry lies the transformative potential of high-throughput screening in advancing drug discovery. Through the ingenious integration of cutting-edge technologies and automation, industry leaders are reshaping the landscape, enhancing efficiency and scaling up processes like never before.
In this exclusive extended webinar, immerse yourself in a world of innovation as our expert speakers unveil state-of-the-art systems and workflows designed to optimize screening assays with unparalleled throughput and reproducibility. Discover how these advancements propel drug discovery pipelines forward with unprecedented speed and precision.
Attend this webinar to:
<ul>
<li>Understand the benefits of integrated high-throughput screening automation in enhancing efficiency and scalability in drug discovery processes</li>
<li>Explore the features of a high-capacity workcell designed for complex compound screens with multiple analytical readouts</li>
<li>Learn about the advantages of a complete walkaway and tipless system in reducing downtime, minimizing waste and accelerating compound screening processes</li>
</ul>
<h3>Agenda</h3>
<h4>TALK 1</h4>
Next-Level Efficiency: Supercharged High-throughput Screening Capacity Through Integrated Automation
Discover how Automata’s cutting-edge platform streamlines drug discovery workflows, boasting a tipless system for seamless operation and enhanced scalability. Learn how the high-capacity workcell empowers researchers to conduct complex compound screens with unparalleled efficiency and reproducibility.
In this session, Alice Tone-Fernandez from Automata will valuable insights into leveraging integrated automation to supercharge high-throughput screening capacity, ultimately revolutionizing efficiency in drug discovery processes.
In her talk, she will:
<ul>
<li>Discuss the benefits of integrated high-throughput screening automation in enhancing efficiency and scalability in drug discovery processes</li>
<li>Explore the features of a high-capacity workcell designed for complex compound screens with multiple analytical readouts</li>
<li>Showcase the advantages of a complete walkaway and tipless system</li>
</ul>
<blockquote>
Presenter: Alice Tome-Fernandez (Senior Application Scientist, Automata)
</blockquote>
<h4>TALK 2</h4>
96 and 384 channel Electronic Pipettes from INTEGRA: Affordable, Simple, Small and Powerful
96 and 384 channel Electronic Pipettes from INTEGRA: Affordable, Simple, Small and Powerful
If you’re working in 24, 96, 384, or even 1536 plate formats, this session will show you 3 instruments which will make you faster, give you more reproducible results, are simple to learn and don’t take up much space - while fitting in every budget. The perfect productivity upgrade for any lab looking to get better.
In this session, Tom Bentivegna from INTEGRA will provide insights into best practices for utilizing these electronic pipettes effectively. Whether you’re a seasoned researcher or new to the field, this talk will provide valuable guidance for optimizing your laboratories workflows.
In his talk, he will:
<ul>
<li>Discuss how to optimize 96 and 384 well-plates to be faster and more reproducible</li>
<li>Showcase novel devices to enhance your high-throughput screening workflows</li>
</ul>
<blockquote>
Presenter: Tom Bentivegna (Group Product Manager, INTEGRA Biosciences)
</blockquote>
<h4>TALK 3</h4>
High-throughput SPR Assay for FcγR Binding to Drug Candidates on the New Carterra LSAXT
Discover the power of high-throughput surface plasmon resonance (HT-SPR) for assessing FcγR binding to drug candidates using the new Carterra LSAXT platform. In this webinar, Peter Hsueh, PhD from ACROBiosystems will showcase how this streamlined approach, coupled with ACROBiosystems’ recombinant FcγR proteins, enables rapid characterization of FcγR interactions across species.
In his talk, he will:
<ul>
<li>Explain the significance of FcγR binding in therapeutic efficacy and cross-reactivity assessment</li>
<li>Exploring a streamlined approach using HT-SPR on the Carterra LSAXT platform</li>
<li>Showcase the benefits of increased sensitivity, reproducibility and time/resource savings in characterizing FcγR interactions compared to traditional SPR methods</li>
</ul>
<blockquote>
Presenter: Peter Hsueh, PhD (Marketing Product Manager, ACROBiosystems)
</blockquote>

Technology Networks: Revolutionizing Drug Discovery: Unleashing the Power of High-Throughput Screening

Revolutionizing Drug Discovery: Unleashing the Power of High-Throughput Screening

Learn about state-of-the-art systems and workflows designed to optimize screening assays with unparalleled throughput and reproducibility.

The Power of High-Throughput Screening

The Right Laboratory Balance Service at the Right Time
Event Program:
<ul>
<li>Selection of the right instrument for your application</li>
<li>Installation and qualification</li>
<li>Preventive maintenance</li>
<li>Calibration</li>
<li>Routine testing</li>
<li>Replacement</li>
</ul>
Weighing is a core process in almost every application in a lab. To maintain consistent results, it&#39;s essential to regularly service your equipment. But what are the most important services, and how often do they need to be performed?
In this webinar, we will guide you, based on the lifecycle of a balance, through the different services that can help you to keep your instrument accurate and extend its lifetime – from the instrument selection to its installation, throughout its routine operation, and up to the time for replacement.
Join our live webinar with METTLER TOLEDO experts to gain a better understanding.
<blockquote>
Presenter: Simona Venzin (Head of Service Product Management, METTLER TOLEDO)
</blockquote>
<blockquote>
Presenter: Walter Krebs (Head of GWP Product Management, METTLER TOLEDO)
</blockquote>

Mettler-Toledo: Service Balances the Smart Way

Service Balances the Smart Way

A guide through different services that can help you to keep your instrument accurate and extend its lifetime.

In this webinar, Dr. Stephanie Marin, Global Clinical Market Development Manager at Phenomenex, will provide a comprehensive overview of mixed-mode polymeric extraction (SPE) fundamentals and the properties of polymeric hydrophobic interaction (reversed-phase) SPE sorbents. Attendees will learn how to choose the appropriate SPE phase for their specific compounds of interest, along with an explanation of the ionic functional groups that provide the mixed-mode functionalities, including both weak and strong cation and anion exchange.
Dr. Marin will also delve into the initial extraction conditions required to initiate method development for each type of SPE sorbent, as well as specialized SPE products tailored for drug testing, phospholipid removal, and microelution SPE. Furthermore, the webinar will include numerous clinical research applications to exemplify these concepts.
Key learning objectives
• Discover how to differentiate different polymeric mixed-mode SPE extraction sorbents • Explore the starting conditions for each sorbent type • Learn how to choose the best SPE sorbent for different analytes • Understand how to use an SPE method development plate
Who should attend?
Scientists involved in SPE method development Labratorians who want to increase their knowledge of polymeric mixed-mode SPE Lab managers who want to implement SPE methods in their laboratories
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
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Presenter: Stephanie J. Marin, PhD (Global Clinical Market Development Manager, Phenomenex)
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<blockquote>
Dr. Stephanie Marin received her Ph.D. in chemistry from Arizona State University and has expertise in sample preparation, LC and LC-MS method development and analysis. She has over 10 years of experience in clinical R&amp;D from her tenure at the ARUP Institute for Clinical and Experimental Pathology. Dr. Marin has worked in multiple laboratory, marketing, and customer-facing roles in the clinical, chromatography consumables, and specialty chemical industries. She is the author of over 30 peer-reviewed publications and book chapters and over 100 abstracts presented at national and international meetings.
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Moderator: Lawrence Howes (Editorial Team, SelectScience)
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Lawrence studied biological sciences at Swansea University, UK, before completing a master’s in epidemiology at the University of Bristol. As a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience.
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SelectScience: Unlocking the Potential of Polymeric Mixed-Mode SPE in Clinical Research

Unlocking the Potential of Polymeric Mixed-Mode SPE in Clinical Research

A comprehensive overview of mixed-mode polymeric extraction (SPE) fundamentals and the properties of polymeric hydrophobic interaction SPE sorbents.

Polymeric Mixed-Mode SPE in Clinical Research

Based on The New ISO 8655 p. 10 for 2024
Event Program
<ul>
<li>Introduction and overview of what&#39;s new in the 2024 ISO 8655 part 10</li>
<li>Requirements and best practices</li>
<li>User qualification and re-qualification</li>
<li>POVA qualifitcation and requalification (routine testing, pass/fail rules, etc)</li>
<li>Solutions to fulfill requirements</li>
</ul>
In this live webinar, we will give you an overview of the ISO 8655 part 10, which was released in March 2024.
You will learn about updated requirements and how the new chapter can affect your lab. Ensure you are up to date with this new regulation while at the same time improving the quality of your results. 
We will also give you an overview of Rainin&#39;s solutions that help you comply with this new chapter of ISO 8655. As this is a webinar you will also have the chance to ask questions to our experts and discuss what aspects need to be considered in comp;iance with the new chapter. 
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Presenter: Irene Ni
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Presenter: Martin Huber
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Presenter: Fabian Troll
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Mettler-Toledo: User Guidance and Qualification in Pipetting

User Guidance and Qualification in Pipetting

In this live webinar, we will give you an overview of the ISO 8655 part 10, which was released in March 2024.

<h4>European Free Educational Webinar Series</h4>
Let&#39;s go back to the basics of Chromatography, Mass Spectrometry, Data Analysis and Trouble-shooting: understanding the importance of the basics will enable you to be more effective in your daily laboratory life.
<h4>EPISODE 1</h4>
Sample Preparation - Theory and Overview
What will you learn?
<ul>
<li>Goals and available Techniques</li>
<li>Intro to Solid Phase Extraction (SPE)</li>
</ul>
<h4>What&#39;s next?</h4>
Full Webinar Series Episodes List is available <a href="https://event.on24.com/wcc/r/4551012/F69479929A905500D8B21508527807E5/5385652">here</a>
Back to Basics - Sample Preparation: Theory and Overview
<ul>
<li>Tuesday, May 07, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Sample Preparation: Tips &amp; Tricks to make it simple
<ul>
<li>Wednesday, June 05, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Theory of Liquid Chromatography
<ul>
<li>Wednesday, July 03, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Basics of Liquid Chromatography
<ul>
<li>Tuesday, September 10, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Analytical Method Development
<ul>
<li>Wednesday, October 02, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - The importance of Compliance
<ul>
<li>Tuesday, October 22, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Introduction to Mass Spectrometry
<ul>
<li>Wednesday, November 13, 2024, 11:00 AM CET</li>
</ul>
Back to Basics - Troubleshooting your LC and MS Systems
<ul>
<li>Tuesday, December 10, 2024, 11:00 AM CET</li>
</ul>
Not sure to be able to attend live sessions?
Register today and watch the webinars on-demand
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Presenter: Xavier Rodriguez (Principal Sales Application Specialist, Waters)
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Waters Corporation: Back to Bacisc Free Educational Webinar Serries

Sample Preparation: Theory and Overview

The first episode will introduce you into goals and available techniques of Solid Phase Extraction (SPE).

<h4>PhenoAcademy Webinars Sample Preparation Series</h4>
Overview of Sample Preparation and Introduction to Filtration
<ul>
<li>Learn about Sample Preparation basics and why it is used</li>
<li>Understand how mechanisms for how samples are cleaned up for analysis</li>
<li>Discover how to select the correct filtration membrane, size and format</li>
</ul>
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Presenter: Laura Ward (Technical Specialist)
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Laura Ward is a Technical Specialist for the Asia-Pacific region with Phenomenex, located in New Zealand. She completed an Honours thesis in the field of Geochemistry at the University of Sydney, Australia where she discovered a love for Analytical Chemistry. Following her academic pursuits, Laura joined Phenomenex Australia as a Technical Sales Consultant where she supported various industries and laboratories. After 5 years and a move to New Zealand, she has joined the Phenomenex Technical Team where she supports scientists globally with their method development, troubleshooting and application support. In her spare time Laura enjoys being outdoors in the New Zealand countryside and cooking from scratch, most recently venturing into cheesemaking!
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Presenter: Lucia Geis Asteggiante (Technical Specialist)
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Lucia is a Senior Technical Specialist that has recently joined Phenomenex’s Global Technical Team. She has a broad experience in the field of Analytical Chemistry with a particular focus on LC chromatography coupled with mass spectrometry. Lucia comes from a strong academic and regulatory background with experiences ranging from method development and analysis of small molecules to large protein complexes. She gained her Ph.D. at the University of Maryland - College Park, USA and did her post-doctoral work at the University of Oxford, UK. During her time as a researcher, she has authored/co-authored 23 scientific publications and 2 book chapters.
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Phenomenex: PhenoAcademy Webinars - Sample Preparation Series

Overview of Sample Preparation and Introduction to Filtration

Sample Preparation basics and why it is used.

Sample Preparation and Introduction to Filtration

<h5>By attending this presentation, you will learn:</h5>
<ul>
<li>Peptide analysis method development via the presentation of experiment results</li>
<li>An understanding of the Biotage sample preparation workflow and the benefits of automation</li>
</ul>
<h5>Event Overview</h5>
An overview of sample preparation approaches for the analysis of World Anti-Doping Agency (WADA) prohibited peptides from urine samples on LC-HRMS (Liquid Chromatography-High Resolution Mass Spectrometry).
The webinar will discuss sample preparation method development approaches undertaken to extract a panel of peptides using the full Biotage workflow. We will address the encountered challenges during development and share insights into optimizing recoveries and ensuring reproducibility for a range of analytes. We will also highlight the benefits of automating these methods to ensure reproducible analysis and free up laboratory analysts&#39; time for other important laboratory tasks.
The webinar is aimed at human and animal sport doping laboratories interested in preparing biofluids for peptide analysis. Attendees will benefit from a deepened understanding of how to achieve clean samples with reproducible results, thereby empowering laboratories to maintain the highest standards of analytical excellence in the field of anti-doping testing.
<h5>What you need to know:</h5>
<ul>
<li>Broadcast #1: 6 June - 10 am BST (London) / 11 am CEST (Paris/Berlin) / 2:30 pm IST (New Delhi) / 5 pm SGT (Singapore)
</li>
<li>Broadcast #2: 6 June - 10 am PDT (Los Angeles) / 12 pm CDT (Chicago) / 1 pm EDT (New York)
</li>
</ul>
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Presenter: Thomas Smith (EU Applications Team Leader, Biotage)
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Thomas has been with Biotage since July 2022 and is supporting customers to improve and streamline their sample preparation approaches and helping them automate their manual, labor-intensive tasks in the laboratory. Before this role, Thomas worked in a toxicology lab analyzing hair for drugs of abuse and alcohol markers. His main role here was to automate a method to increase reproducibility and turnaround time to meet the growing sample numbers.
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Separation Science: Novel Method Development Approaches for Peptide Analysis in Doping

Novel Method Development Approaches for Peptide Analysis in Doping

The webinar is aimed at human and animal sport doping laboratories interested in preparing biofluids for peptide analysis.

LCMS webinars focusing on Sample prep - page 20