<div>Mulching films used in agriculture gradually break down and can become a significant source of microplastics in soils. A new study shows that these particles affect not only soil structure but also microbial life and nitrogen cycling.“We wanted to understand how microplastics from both conventional and biodegradable mulching films influence soil properties, microbial diversity and functions in real conditions,” explains Klára Šmídová, the study’s co-author . “That’s why we added realistic amounts of microplastics to soils in experimental plots in Finland, Germany, and Spain, monitored them over two growing seasons, and compared them with the control plots.”The results revealed that microplastics alter soil characteristics and microbial communities, although not always in the same way. “ However, a consistent finding was the decline in microbial activity associated with key processes, including nitrogen cycling, with negative effects increasing at higher microplastic concentrations and becoming more pronounced in the second year,” Šmídová adds. Because the microplastic levels used in the study reflect amounts already found in European soils, the findings highlight real risks to soil health. “We also found that the effects were stronger in southern than in northern Europe, likely due to differences in climate and soil conditions. This means that protecting soils from plastic pollution will require region-specific risk assessments,” she says.Over 60% of European soils are estimated to be unhealthy, with ongoing degradation driven by unsustainable land use, contamination, climate change, and extreme weather events. In response, the EU Parliament adopted the Soil Monitoring Law in October 2025, aiming to achieve healthy soils across Europe by 2050. “The law highlights the role of soil health in food security, water quality, and climate objectives, and establishes consistent soil monitoring across Member States. It includes measures to support farmers and foresters, while also creating a public list of potentially contaminated sites and an indicative watch list of emerging soil contaminants,” explains Klára Šmídová.The project brought together 19 research groups across Europe. “After each season, hundreds of samples were colle cted and sent to partners for analysis. This paper focuses on soil properties, microbial diversity, and greenhouse gas emissions. Two more manuscripts—on plastic additives and microplastic fate, and on soil invertebrates—are in preparation. Together, they will give us a complete picture of how agricultural microplastics affect soil ecosystems,” concludes Klára Šmídová.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/RECETOX_Vysledky_nove_studie_ukazaly_ze_mikroplasty_meni_vlastnosti_pudy_a_mikrobialni_spolecenstva_i_v_realnych_podminkach_6ee3413a7b.jpg" alt="RECETOX: New Study Reveals How Agricultural Plastics Impact Soil Health In Real Conditions" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_RECETOX_Vysledky_nove_studie_ukazaly_ze_mikroplasty_meni_vlastnosti_pudy_a_mikrobialni_spolecenstva_i_v_realnych_podminkach_6ee3413a7b.jpg 171w," sizes="100vw" width="738" height="674"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0269749125015866?via%3Dihub#sec2">Conventional and biodegradable agricultural microplastics: effects on soil properties and microbial functions across a European pedoclimatic gradient</a></h4>Šmídová K., Soinne H., Kim S. W., Tirronniemi J., Meffe R., Redondo-Hasselerharm P. E., Braun M., Rillig M. C., Fritze H., Adamczyk B., Nikama J., Kaseva J., Saartama V., Amelung W., Hurley R., Hofman J., Nizzetto L., Selonen S., Velmala S.Environ. Pollut. 386 (2025) 127212<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.envpol.2025.127212">10.1016/j.envpol.2025.127212</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4><div>Agricultural plastics like mulching films may become a major source of microplastic (MP) soil contamination during their degradation and fragmentation. This study investigates the effects of agricultural MPs from conventional (linear low-density polyethylene, PE) and biodegradable (starch-blended polybutylene adipate co-terephthalate, PBAT-BD) mulching films on soil physicochemical properties, aggregation, microbial diversity and functions, litter decomposition, and greenhouse gases emissions (GHG). For this purpose, MPs were mixed into soils at realistic MP concentrations of 0.005&nbsp;% and 0.05&nbsp;% (w/w) in 2022 on experimental plots in three EU countries representing different pedoclimatic conditions (Finland, Germany and Spain), followed by monitoring of the above-mentioned variables in the subsequent growing seasons 2022 and 2023. We found several significant MP-induced effects for soil properties, aggregation, microbial diversity, litter decomposition, and GHG, but the effect endpoints were less pronounced or varied considerably. Contrarily, microbial activity, contributing to soil functions such as nitrogen cycling, was consistently reduced by both conventional and biodegradable MPs. The reductions were more pronounced after the second season and for the higher MP treatment. As the higher MP concentration (i.e., 0.05&nbsp;% w/w) is environmentally relevant in Europe, our findings emphasize the potential effects of environmentally relevant MP concentrations on soil health. Furthermore, the effects increased from north to south, probably modulated by varying pedoclimatic conditions, inducing reflection of a need for regionally tailored risk</div></div>

New Study Reveals How Agricultural Plastics Impact Soil Health In Real Conditions

<h3>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</h3>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711">Among the analytical techniques available today, anion exchange chromatography (AEX) stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</a></blockquote><h4>How Scientists Read Oligonucleotides Using Charge</h4>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.<h4>Why Ion Exchange Works: Binding, Retention, and Release</h4>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, anion exchange is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.The system consists of two main components:<ul><li>Stationary phase: A positively charged resin that binds anionic analytes.</li><li>Mobile phase: An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"><h4>The Five-Step Workflow of Ion Exchange Chromatography</h4>AEX separations generally follow a well-established sequence of steps:<ol><li>Column equilibration The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li>Sample loading Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li>Washing The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li>Elution Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li>Regeneration and re-equilibration A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2 Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"><h4>Why AEX Is Especially Effective for Oligonucleotides</h4>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.Key factors influencing AEX behavior include:<ul><li>Charge density: Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li>Resin chemistry: Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li>Buffer composition: The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"><h4>AEX as the Gold Standard for Oligonucleotide Separation</h4>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:<ul><li>High resolving power: Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li>Predictable retention behavior: In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li>Compatibility with modified oligonucleotides: Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li>Excellent control via gradient elution: Salt gradients provide precise tuning of selectivity and resolution.</li></ul>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"><h4>Final Perspective: Mastering AEX with Confidence</h4>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.<blockquote>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at sales@knauer.net. More insights into practical oligonucleotide analysis will follow in our “Oligos Made Easy” series.For technical questions or in-depth discussion, the author can be reached at kindler@knauer.net</blockquote>

Oligos Made Easy - Part 2: AEX

News from LabRulezLCMS Library - Week 07, 2026

Article

Application

Plastics

Environment

Science and research

Poster

RECETOX (Research Centre for Toxic Compounds in the Environment) is an independent department of the Faculty of Science of Masaryk University in Brno, which has been established in 1983. RECETOX is focused on the research and education in cross-cutting area of Environment and Health. It addresses interactions among chemicals, environment, and biological systems, including consequences at local, regional, and global levels.

RECETOX - Centrum pro výzkum toxických látek v prostředí

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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PhenoAcademy Webinars
 Liquid Chromatography Series
<h4>HPLC Troubleshooting</h4>
<ul>
<li>Learn how to prevent and minimize your LC problems before they occur</li>
<li>Understand how to approach problem solving and troubleshooting in a logical way, including: diagnose cause and possible solutions</li>
<li>Review peak shape, and column performance problems</li>
</ul>
<blockquote>
Presenter: Kevin Zhao (Senior Technical Specialist)
</blockquote>
<blockquote>
Kevin Zhao is the Senior Technical Specialist with Phenomenex and has 5 years of experience in biochemistry research; 4 years of Protein/Cancer research with CSULA-City of Hope Cancer Collaborative and over 9 years in Phenomenex. Mr. Zhao is a member of a global Technical Support Team which includes providing problem-solving, troubleshooting, and applications assistance for customers worldwide in the fields of chromatography (liquid and gas) and sample preparation, and also handling Industries supported include – pharmaceutical and biopharmaceutical, clinical research and clinical toxicology, food safety, environmental, fuels and specialty chemicals. Mr. Zhao received his B.S. in Biology at the California State Polytechnic University, Pomona. earned his M.S. in Biochemistry at California State University, Los Angeles, and also earned a MBA at the University of the Incarnate Word.
</blockquote>
<blockquote>
Presenter: Koen Askamp (Technical Specialist)
</blockquote>
<blockquote>
Koen is from the Netherlands, studied Molecular Life Sciences at Wageningen University (Netherlands), majoring in immunology. Analytical Chemistry was one of my favorite courses – who doesn’t love solving puzzles?
</blockquote>
<blockquote>
I like drumming, scuba diving, motorcycle-riding, and this past winter I learned how to ski (a little)!
</blockquote>
<blockquote>
Presenter: Namrata Saxena (Technical Manager)
</blockquote>
<blockquote>
Namrata Saxena is a Technical Manager - Asia/Pacific” with Phenomenex and is based in Sydney, Australia. She has a post graduate degree with a specialization in Analytical Chemistry, with extensive experience in the Liquid chromatography domain, and her current focus is in Omics and Biologics field. 
</blockquote>
<blockquote>
Before joining Phenomenex she had worked as “Product Specialist” and spent several years in column chemistry, column manufacturing and method development in the application and manufacturing labs of Bischoff Chromatography, Germany. 
</blockquote>
<blockquote>
In her current role, she provides product selection, method development, training and troubleshooting support to customers as well as Phenomenex Sales Consultants globally. 
</blockquote>

Phenomenex: PhenoAcademy Webinars - Liquid Chromatography Series

HPLC Troubleshooting

Learn how to prevent and minimize your LC problems before they occur.

<h4>LC-MS/MS fundamentals webinar series 2.0</h4>
Advanced techniques for data acquisition and processing
Elevate your analytical expertise with advanced techniques and workflows
Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series. Based on our LC-MS/MS 1.0 webinar series, these sessions will go beyond the fundamentals of LC-MS/MS and introduce ways to advance your analytical methods and data processing workflows.
Every other week, we will explore how to optimize SCIEX OS software with short, 30-minute expert-led presentations.
<h4>This webinar:</h4>
June 4, 2024 11:00 a.m. - 11:30 a.m. CET - Mastering chromatography
Discover how to optimize your chromatography for even the most challenging compounds while creating and building LC methods.
<h4>Other webinars from the series:</h4>
May 7, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.cz/webinar/3820">Beyond the fundamentals</a>
May 21, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3852">Advanced MS/MS method building</a>
Gain insight into advanced features for maximizing sensitivity in your mass spectrometry analyses.
June 18, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3854">It&#39;s a TRAP! Getting the most out of your QTRAP system</a>
Learn when to utilize QTRAP functionality to create more selective and sensitive methods.
July 2, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3855">Troubleshooting at the source</a>
Acquire the knowledge needed to optimize source parameters and identify and resolve ionization-related problems.
July 16, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3856">Automating data processing</a>
Learn how to automate your data processing with custom flagging and rules for efficient data review in SCIEX OS software.
July 30, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3857">Excel strategies for mass spectrometry data analysis</a> Take an in-depth look at when and how to use Excel to increase your productivity.

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Mastering chromatography

Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series.

The successful regulatory filing of any pharmaceutical product development dossier relies on scientific and rational way of analytical method development.
There are many analytical development strategies which can be followed like “Traditional” or “Enhanced approach”. Enhanced Approach as stated by ICH, or DoE based Quality-by-Design (QbD) approach are one of such method development strategies which will be discussed in webinar. These approaches are now part of regulations across USP, ICH and USFDA. We will also discuss the different concepts like ATP, Lifecycle Management, Risk Assessment etc. Join us for this webinar to understand different guidelines related to analytical development and how can you adopt QbD or Enhanced approach in the analytical method development.
<blockquote>
Presenter: Manu Grover (Business Development Manager – Pharma Markets, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Manu Grover holds a postgraduate degree in Pharmacy from Manipal Academy, India. He has more than 21 years of chromatography experience and has worked in pharmaceuticals to support IND, NDA, and ANDA filings incompanies such as Dr. Reddy&#39;s, Panacea Biotec, and Ozone Pharmaceuticals. During his professional journey, he has developed and validated analytical methods on HPLC, GC, particle sizing, DSC, pXRD, and LC/MS instruments, as well as successfully filing multiple analytical dossiers in U.S./EU markets. In his current role, he works closely with the Agilent sales team, regulators, and pharmaceutical scientists. He shares market updates, trends, and expertise related tothe Agilent portfolio of columns and supplies.
</blockquote>

Agilent: Becoming a Better Chromatographer - Educational Webinar Series

Regulatory Guidelines on Analytical Method Development: USFDA, ICH and USP Perspective

Understand different guidelines related to analytical development and how can you adopt QbD or Enhanced approach in the analytical method development.

Regulatory Guidelines on Analytical Method Development

Sample-to-sample and run-to-run reproducibility in chromatography is of utmost importance.
What do you do when your chromatography suddenly changes?
In this webinar, we’ll discuss some of the most common chromatography issues that we see as technical support specialists. Whether you have retention time changes, pressure changes, new peaks, ghost peaks, or peak shape changes, troubleshooting these issues can be a challenge. We’ll provide explanations for why these changes may be happening and make recommendations for how to avoid them in the future.
<blockquote>
Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
</blockquote>

Agilent Technologies: My Chromatography Has Changed: Steps for Effective Troubleshooting

My Chromatography Has Changed: Steps for Effective Troubleshooting

We’ll discuss some of the most common chromatography issues that we see as technical support specialists.

Steps for Effective HPLC Troubleshooting

HPLC is a powerful analytical technique widely used in the food industry, research labs and commercial testing labs for quality control, safety assessment, and nutritional analysis. It is particularly valuable for analyzing compounds such as vitamins, amino acids, sugars, oils and toxins like mycotoxins. HPLC plays a crucial role in ensuring the safety, quality, and nutritional integrity of food products. Its ability to provide detailed information about the composition of food samples makes it an indispensable tool for food testing laboratories and the food industry.
Altium is happy to announce webinar on Agilent LC solutions for food industry. Reserve your spot now!
<a href="https://events.teams.microsoft.com/event/ecf82ef6-1be9-404d-8dda-732d993c8699@3a2a17c9-a748-438b-8c0e-b375b3713c75">REGISTRATION</a>
Key learning points:
<ul>
<li>HPLC introduction.</li>
<li>Key HPLC features which can reduce human error and enhance efficiency.</li>
<li>HPLC applications for vitamins analysis.</li>
<li>HPLC applications for amino acid analysis using automated pre column derivatization method.</li>
<li>HPLC application for sugar analysis.</li>
<li>HPLC application for aflatoxin analysis.</li>
<li>GPC as a technique of sample prep for removing high molecular matrix.</li>
</ul>
There will be a Q&amp;A session with the support of local Altium teams.
Looking forward to meeting you.
<blockquote>
Speaker: Laura Montis (IDO Product Specialist LPS Agilent Technologies)
</blockquote>

Altium International: Agilent LC Solutions For Food Testing and Food Fortification Webinar

Agilent LC Solutions For Food Testing and Food Fortification Webinar

HPLC is widely used in the food industry, research labs and commercial testing labs for quality control, safety assessment, and nutritional analysis.

Agilent LC Solutions For Food Testing and Food Fortification

<h4>Waters 2024 LC Symposium</h4>
Pushing the Boundaries of LC to Address Today’s Challenges
<h5>Overview</h5>
Today’s scientists and lab managers need to confidently deliver accurate, reliable, and reproducible data while facing increasingly complex molecules, aggressive sustainability goals, and accelerated timelines. 
Join this free symposium to get insights from global LC thought leaders on the advancements of LC technology to address current challenges. 
<h3>Day 1 – June 12 (10:00 AM EDT | 15:00 PM BST | 16:00 PM CEST)</h3>
<h5>Advanced LC Technologies for Increasingly Complex Molecules</h5>
Our panel of industry leaders will discuss recent developments in liquid chromatography designed to meet today&#39;s analytical challenges. Our speakers will explore innovative technologies to mitigate unpredictable chromatography due to non-specific adsorption and explore the insights from light scattering detection.
<h5>Learning objectives</h5>
<ul>
<li>Hear from LC thought leaders about state-of-the-art LC technologies </li>
<li>Learn how to control metal-based adsorption</li>
<li>Explore orthogonal detection schemes</li>
<li>Discover the next generation of intuitively simple LC systems</li>
</ul>
Join us for Waters 2024 LC Symposium, we are really looking forward to answering your questions in our live Q&amp;A session and discussing this topic further with you.
<blockquote>
<h3>Day 2 – June 13 (10:00 AM EDT | 15:00 PM BST | 16:00 PM CEST)</h3>
</blockquote>
<blockquote>
<h5>Next Generation HPLC: the Quest for Performance and Simplicity</h5>
</blockquote>
<blockquote>
Advancements in HPLC technology impact routine testing laboratories where error reduction, ease of use and system performance play a crucial role. Our expert panel will discuss recent advances in HPLC technology, revisions to the USP &lt;621&gt; guidelines, application of Analytical Quality by Design (AQbD) software to assess method robustness, and innovative technologies which enhance simplicity and performance.
</blockquote>
<blockquote>
Presenter: Davy Guillarme, Ph.D. (Sr Lecturer and Research Associate, University of Geneva)
</blockquote>
<blockquote>
Davy Guillarme, holds a Ph.D. in analytical chemistry from the University of Lyon, France, currently serves as a senior lecturer and research associate at the University of Geneva in Switzerland. With over 340 published journal articles in pharmaceutical analysis and h-index of 75, his expertise spans various techniques, including HPLC, UHPLC, HILIC, LC−MS, SFC, SFC-MS, multidimensional LC, and the analysis of biopharmaceuticals (proteins, mAbs, oligonucleotides, gene therapy products, mRNA…).
</blockquote>
<blockquote>
He is an associate editor of the Journal of Chromatography A and has been honored with the LC-GC Emerging Leader Award in Chromatography (2013), the Jubilee Medal from the Chromatographic Society (2018), and the International Award of the Belgian Society of Pharmaceutical Sciences (2022). His global recognition extends to being listed among the world&#39;s most influential analytical scientists by &quot;Analytical Scientist&quot; magazine in multiple years.
</blockquote>
<blockquote>
Presenter: Guilherme (Gui) Guimaraes, Ph.D. (Scientist, Biogen)
</blockquote>
<blockquote>
Dr. Guilherme (Gui) Guimaraes is a scientist in drug metabolism and pharmacokinetics at Biogen. He is responsible for bioanalytical analysis of small molecules and antisense oligonucleotides. Gui holds a Ph.D in pharmaceutical and biomedical sciences and a masters in international biomedical regulatory sciences from the University of Georgia. His graduate work was focused on the application of novel analytical strategies to improve the chromatographic performance and sensitivity of oligonucleotide LC-MS methods. Some of these strategies include the use of low adsorption surfaces and microflow LC-MS.
</blockquote>
<blockquote>
Presenter: Michelle Chen, Ph.D. (Sr Director, Research and Development, Waters l Wyatt Technology)
</blockquote>
<blockquote>
Dr. Michelle Chen is a senior director at Waters | Wyatt Technology located in Santa Barbara, California. Michelle obtained her Ph.D. from the Chemical Engineering Department at Yale University, where she focused on developing novel HPLC approaches for high-speed and high-efficiency separations of biopolymers. Since joining Wyatt, Michelle has incorporated multi-angle light scattering and dynamic light scattering detection with HPLC and field-flow fractionation, for characterizing synthetic and biological polymers, proteins, and nanoparticles. In recent years she has led the application team at Wyatt Technology to develop novel methods to characterize and quantify emerging therapeutics and delivery vehicles including new protein conjugates, viral vectors, lipid nanoparticles, and extracellular vesicles.
</blockquote>
<blockquote>
Presenter: Moderated by: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)
</blockquote>
<blockquote>
Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.
</blockquote>

Waters Corporation: Waters 2024 LC Symposium

Advanced LC Technologies for Increasingly Complex Molecules

Free symposium to get insights from global LC thought leaders on the advancements of LC technology to address current challenges.

<h4>Waters 2024 LC Symposium</h4>
Pushing the Boundaries of LC to Address Today’s Challenges
<h5>Overview</h5>
Today’s scientists and lab managers need to confidently deliver accurate, reliable, and reproducible data while facing increasingly complex molecules, aggressive sustainability goals, and accelerated timelines. 
Join this free symposium to get insights from global LC thought leaders on the advancements of LC technology to address current challenges. 
<h3>Day 2 – June 13 (10:00 AM EDT | 15:00 PM BST | 16:00 PM CEST)</h3>
<h5>Next Generation HPLC: the Quest for Performance and Simplicity</h5>
Advancements in HPLC technology impact routine testing laboratories where error reduction, ease of use and system performance play a crucial role. Our expert panel will discuss recent advances in HPLC technology, revisions to the USP &lt;621&gt; guidelines, application of Analytical Quality by Design (AQbD) software to assess method robustness, and innovative technologies which enhance simplicity and performance.
Learning objectives
<ul>
<li>Hear from LC thought leaders about state-of-the-art LC technologies </li>
<li>Learn how to control metal-based adsorption</li>
<li>Explore orthogonal detection schemes</li>
<li>Discover the next generation of intuitively simple LC systems</li>
</ul>
Join us for Waters 2024 LC Symposium, we are really looking forward to answering your questions in our live Q&amp;A session and discussing this topic further with you.
<blockquote>
<h3>Day 1 – June 12 (10:00 AM EDT | 15:00 PM BST | 16:00 PM CEST)</h3>
</blockquote>
<blockquote>
<h5>Advanced LC Technologies for Increasingly Complex Molecules</h5>
</blockquote>
<blockquote>
Our panel of industry leaders will discuss recent developments in liquid chromatography designed to meet today&#39;s analytical challenges. Our speakers will explore innovative technologies to mitigate unpredictable chromatography due to non-specific adsorption and explore the insights from light scattering detection.
</blockquote>
<blockquote>
Presenter: Davy Guillarme, Ph.D. (Sr Lecturer and Research Associate, University of Geneva)
</blockquote>
<blockquote>
Davy Guillarme, holds a Ph.D. in analytical chemistry from the University of Lyon, France, currently serves as a senior lecturer and research associate at the University of Geneva in Switzerland. With over 340 published journal articles in pharmaceutical analysis and h-index of 75, his expertise spans various techniques, including HPLC, UHPLC, HILIC, LC−MS, SFC, SFC-MS, multidimensional LC, and the analysis of biopharmaceuticals (proteins, mAbs, oligonucleotides, gene therapy products, mRNA…).
</blockquote>
<blockquote>
He is an associate editor of the Journal of Chromatography A and has been honored with the LC-GC Emerging Leader Award in Chromatography (2013), the Jubilee Medal from the Chromatographic Society (2018), and the International Award of the Belgian Society of Pharmaceutical Sciences (2022). His global recognition extends to being listed among the world&#39;s most influential analytical scientists by &quot;Analytical Scientist&quot; magazine in multiple years.
</blockquote>
<blockquote>
Presenter: Guilherme (Gui) Guimaraes, Ph.D. (Scientist, Biogen)
</blockquote>
<blockquote>
Dr. Guilherme (Gui) Guimaraes is a scientist in drug metabolism and pharmacokinetics at Biogen. He is responsible for bioanalytical analysis of small molecules and antisense oligonucleotides. Gui holds a Ph.D in pharmaceutical and biomedical sciences and a masters in international biomedical regulatory sciences from the University of Georgia. His graduate work was focused on the application of novel analytical strategies to improve the chromatographic performance and sensitivity of oligonucleotide LC-MS methods. Some of these strategies include the use of low adsorption surfaces and microflow LC-MS.
</blockquote>
<blockquote>
Presenter: Michelle Chen, Ph.D. (Sr Director, Research and Development, Waters l Wyatt Technology)
</blockquote>
<blockquote>
Dr. Michelle Chen is a senior director at Waters | Wyatt Technology located in Santa Barbara, California. Michelle obtained her Ph.D. from the Chemical Engineering Department at Yale University, where she focused on developing novel HPLC approaches for high-speed and high-efficiency separations of biopolymers. Since joining Wyatt, Michelle has incorporated multi-angle light scattering and dynamic light scattering detection with HPLC and field-flow fractionation, for characterizing synthetic and biological polymers, proteins, and nanoparticles. In recent years she has led the application team at Wyatt Technology to develop novel methods to characterize and quantify emerging therapeutics and delivery vehicles including new protein conjugates, viral vectors, lipid nanoparticles, and extracellular vesicles.
</blockquote>
<blockquote>
Presenter: Moderated by: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)
</blockquote>
<blockquote>
Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.
</blockquote>

Next Generation HPLC: the Quest for Performance and Simplicity

Next Generation HPLC: Performance and Simplicity

Join us for thought provoking webinar series on the entire value chain of therapeutic peptides.
Register now and get an opportunity to learn about:
<ul>
<li>Peptide manufacturing specifications</li>
<li>Raw materials and intermediates</li>
<li>USP general chapters and regulatory considerations</li>
<li>Chromatographic strategies for optimizing peptide analysis and purification with reversed phase chromatography and suitable stationary phases</li>
<li>End-to-end analytical to preparative workflow for synthetic peptides</li>
</ul>
<h5>Untold Control Strategies in Complex Peptide Manufacturing</h5>
<ul>
<li>Dr. Vasanthakumar G. Ramu</li>
</ul>
<h5>Regulatory Considerations for Peptides Therapeutics</h5>
<ul>
<li>Dr. Manoj Kumar Metta</li>
</ul>
<h5>Analysis and Purification of Synthetic Peptides</h5>
<ul>
<li>Ms. Paramjeet Khandpur</li>
</ul>
<blockquote>
Presenter: Dr. Vasanthakumar G. Ramu (Vice President – Head R&amp;D, Peptides and Complex API’s, Alembic Pharmaceutical Ltd.)
</blockquote>
<blockquote>
Dr. Vasanthakumar Ramu, is one of the pragmatic scientists in the pharma industry working around complex API’s. He studied chemical biology from Bangalore University and Indian Institute of Science. In early 2007, hemoved to Max-Planck Institute of Molecular Physiology to work on peptide decorated supramolecular architectures as modulators to study cell protein interactions. Continued further studies as research scientist in Ruhr Universität Bochum, Germany, with the objective to work on backbone modified peptides of HIV protease inhibitors. In 2011 spring, moved to Spain, where he was working as Marie Curie Experienced Researcher to develop Kyotorphin derived peptides, antimicrobial peptides, and other small molecules for neurodegeneration. Returned to India in 2012 and led the startup company called Omgene Life Sciences Pvt. Ltd. Before joining Alembic, was heading peptide process development groups in Biocon Ltd., and Aurobindo Pharma Ltd., Transferred the technologies, successfully validated and filed various DMF’s and involved in commercialization of API’s.
</blockquote>
<blockquote>
His work was centered on developing diverse peptides and complex therapeutic API’s to treat T2D and various other health ailments. Published more than 30 peer reviewed scientific articles and 50+ patent applications filed/granted in various IP jurisdictions. Dr. Vasanthakumar’s efforts have been recognized through Marie Curie and Max Planck Institute fellowships. He is fellow of Chemical Research Society of India (CRSI) and Nuclear Magnetic Resonance Society of India (NMRS) and recently, he has been invited as Fellow of Royal Society of Chemistry, London in leadership category in recognition of his contributions in chemical sciences. Presently, heading R&amp;D, and leading end to end development and technology transfer activities for various peptides and complex generic API’s.
</blockquote>
<blockquote>
Presenter: Dr. Manoj Kumar Metta (Team Lead, Senior Scientist II Global Biologics, United States Pharmacopeia (USP))
</blockquote>
<blockquote>
Dr. Manoj Metta is a renowned Biological Scientist with 21 years of experience in Biologics Quality assessment, and Regulatory sciences. He has rich hands-on experience in commercial development of protein, and peptide biopharmaceutical products with emphasis on developing analytical control strategies, physicochemical and functional characterization, analytical method development/validations and CMC review to support product licensure in global markets.
</blockquote>
<blockquote>
Dr. Manoj brings a wealth of experience and expertise to his role at the US Pharmacopeia (USP), where he has served for over 12 years in various scientific capacities. His contributions span across multiple areas, including the development of Monographs, Reference standards and General chapters in therapeutic proteins, monoclonal antibodies, synthetic peptides, and oligonucleotides. Dr. Manoj is a frequent speaker at national and international biopharmaceutical and regulatory scientific events.
</blockquote>
<blockquote>
Academically, he received Ph.D. degree in Biotechnology from Gandhi Institute of Technology and Management, on “Development of novel mammalian expression systems by using polyomaviral elements in CHO cell line” for monoclonal antibody production”.
</blockquote>
<blockquote>
Presenter: Ms. Paramjeet Khandpur (Biopharma Workflow Commercialization Lead. Agilent Techologies)
</blockquote>
<blockquote>
Paramjeet Khandpur, M.Tech., holds a bachelor’s degree in chemical engineering from VTU University and a Master&#39;s in Bioprocess Technology from the Institute of Chemical Technology, Mumbai. Paramjeet is currently the Biopharma Workflow Commercialization Lead at Agilent. In this role, she leverages her expertise to deliver comprehensive workflow solutions and offer support to customers, catering to their analytical requirements across the Biopharma value chain.
</blockquote>
<blockquote>
Previously, she worked as an Application Engineer at Agilent&#39;s Centre of Excellence Lab in Bangalore, specializing in various liquid chromatography platforms. She also collaborated with users in the Pharma and Biopharma industry for method development, troubleshooting, and method transfer.
</blockquote>

Agilent: Advance Your Peptide Product Commercialization: CMC and Regulatory Considerations

Advance Your Peptide Product Commercialization: CMC and Regulatory Considerations

Webinar series on the entire value chain of therapeutic peptides.

Peptide Product Commercialization: CMC and Regulatory

This webinar unveils the development of a high-throughput multidimensional liquid chromatography approach in combination with charged aerosol detection (CAD), leveraging a combination of ion exchange and reversed-phase columns, ensuring accurate and precise analysis within minutes.
Discover how this method advances formulation development, stability testing, and batch release, providing researchers with valuable insights into surfactant degradation dynamics. 
Learning Points: 
<ul>
<li>Discover cutting-edge techniques for rapid analysis of surfactants in antibody formulations</li>
<li>Unveil high-throughput liquid chromatography with charged aerosol detection</li>
<li>Gain valuable insights for optimizing formulation development and stability testing</li>
</ul>
Who should attend?
<ul>
<li>Biopharmaceutical scientists and researchers specializing in monoclonal antibody development</li>
<li>Analytical chemists and technicians involved in pharmaceutical formulation analysis</li>
<li>Quality control and regulatory affairs professionals in the pharmaceutical industry</li>
</ul>
<blockquote>
Presenter: Maksymilian Zegota, PhD (Senior Scientist, Coriolis Pharma)
</blockquote>
<blockquote>
Dr. Zegota earned his PH.D. in chemistry from the Max Planck Institute for polymer research in Mainz, Germany, concentrating on site-selective protein modification.
</blockquote>
<blockquote>
After completing his doctorate, he joined Coriolis Pharma in Martinsried, Germany.
</blockquote>
<blockquote>
Currently, Dr. Zegota focuses on developing Novel methods for surfactant analysis in biologics to assist in the root-cause analysis of polysorbate and poloxamer degradation, contributing to formulation development in the pharmaceutical field.
</blockquote>
<blockquote>
Presenter: Marcus Klee (Marcus Klee, Regional Marketing Manager HPLC, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Graduated in Chemical Engineering from Hochschule Fresenius in 2000, specialized in Marketing &amp; Sales. Since then, held various marketing roles since 2000, currently serving as the Regional Marketing Manager EMEA for HPLC
</blockquote>

Thermo Scientific: Advancing analytical techniques in monoclonal antibody formulations

Advancing analytical techniques in monoclonal antibody formulations

Explore cutting-edge methodology for online protein removal and characterization of polysorbates and poloxamer in monoclonal antibody formulations.

Analytical techniques in monoclonal antibody formulations

Advances in monoclonal antibody (mAb) engineering have led to additional challenges in the purification of these modalities, including high levels of aggregates. Ion exchange chromatography is often not sufficient to achieve purity targets meaning hydrophobic interaction chromatography (HIC) and mixed-mode resins are needed as orthogonal tools. These technologies can be implemented in flow-through mode allowing for high loadings and fast processing times, making them well-suited for large-scale implementation.
This webinar highlights three case studies that illustrate how novel chromatographic tools can improve the purification process for complex therapeutics by reducing the levels of aggregates and host cell proteins (HCPs) and effectively clearing retroviruses.
Attend this webinar to explore case studies on:
<ul>
<li>Effective aggregate removal and viral clearance via HIC flow-through unit operation</li>
<li>High molecular weight reduction and viral clearance for a novel, pre-conjugated mAb for ADC production</li>
<li>Simultaneous HCP reduction and aggregate removal with mixed-mode chromatography for a high aggregate mAb feedstream</li>
</ul>
<blockquote>
Presenter: Robert Stairs (Field Applications Staff Scientist, Thermo Fisher Scientific)
</blockquote>

Technology Networks: Advances in Flow-Through Technology To Enhance mAb Polishing

Advances in Flow-Through Technology To Enhance mAb Polishing

Case studies that illustrate how novel chromatographic tools can improve the purification process for complex therapeutics.

LCMS webinars focusing on HPLC - page 22