Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

News from LabRulezLCMS Library - Week 20, 2026

🎤Lindsay Repka&nbsp;Lindsay Repka is a chemistry professor at Middlebury College whose research explores photoredox chemistry, catalyst stability, and visible-light-driven transformations. Her lab emphasizes both mechanistic insight and hands-on student training in advanced analytical instrumentation.In this episode of Concentrating on Chromatography, we sit down with Lindsay Repka to discuss how LC-MS and GC-MS transformed her lab’s approach to photoredox chemistry.What began as a project to develop a visible-light photocrosslinking handle unexpectedly led to a major discovery: the solvent (DMF) was reacting with the photocatalyst itself. Using high-resolution LC-MS, Lindsay’s team observed multiple solvent adducts forming — sometimes with complete catalyst consumption. That discovery reshaped their research direction.This episode is a rare deep dive into both LC-MS and GC-MS within the same research project, showing how chromatography-driven insight can turn unexpected degradation into productive new reactivity.If you work in:<ul><li>Photoredox chemistry</li><li>Reaction optimization</li><li>Mass spectrometry method development</li><li>Catalyst screening</li><li>Academic synthetic chemistry</li></ul>…this conversation will resonate.<h4>Key Topics Covered</h4><ul><li>Photocatalyst stability in DMF, DCE, and MeCN</li><li>Demethylation under mild visible-light conditions</li><li>High-resolution Q-TOF LC-MS quantitation</li><li>Internal standard methodology in GC-MS</li><li>Signal-to-noise improvement using extracted ion chromatograms</li><li>Reaction reproducibility and quality control strategy</li></ul><h3>Video Transcription</h3>In this interview, Lindsay Repka discusses the role of photoredox chemistry in method development, photocrosslinking, solvent activation, catalyst stability, and the use of LC-MS and GC-MS to understand reaction pathways.<h4>What is photoredox chemistry?</h4>Photoredox chemistry uses light to activate a photocatalyst, which can either donate or accept an electron from a substrate. This generates radical species that can undergo new types of reactivity.Repka explains that one of the key advantages of photoredox chemistry is its ability to operate under mild conditions, often using visible light. This makes it attractive for sensitive molecules and biological systems, including potential applications in drug release, live-cell chemistry, and drug development.<h4>Photocrosslinking and drug-target interactions</h4>Repka’s group initially aimed to develop visible-light photocrosslinking methods to study drug interactions with biological targets. Photocrosslinking can covalently link a drug to its target, making it easier to identify the biological target of a compound.Traditional photocrosslinking often uses UV light, but visible light offers milder and more biologically compatible conditions.<h4>From photocrosslinking to solvent activation</h4>The project shifted direction when the desired photocrosslinking reactions gave very low yields. Instead of reacting with amino acids as intended, the radical intermediate mainly reacted with itself.The team explored different solvents to stabilize the radical, but found that some solvents, such as DMF, were not inert. Instead, the solvent reacted with the photocatalyst or radical species, revealing unexpected solvent activation chemistry.<h4>Why LC-MS was critical</h4>LC-MS became essential because the reaction mixtures were too complex and low-yielding for NMR or GC-MS. High-resolution LC-MS allowed the team to detect minor products, identify photocatalyst modifications, and observe solvent addition products with high confidence.Repka emphasizes that LC-MS was both a qualitative and quantitative tool. It was used quantitatively to measure how much photocatalyst remained after light exposure, and qualitatively to identify degradation products and new reactivity pathways.<h4>Photocatalyst stability workflow</h4>The team developed a systematic workflow to evaluate photocatalyst stability under light exposure. Because catalyst degradation was time-sensitive, reproducibility required strict control of experimental conditions.Key steps included using a glove box, commercial deoxygenated solvents, accurate weighing on a microbalance, controlled light exposure in a photoreactor, and temperature control with a chiller. These precautions helped reduce day-to-day variability and enabled reproducible measurements of catalyst remaining.<h4>Quantifying catalyst remaining by LC-MS</h4>Calibration curves were prepared using pure photocatalyst solutions and LC-MS ion areas. The team worked in the nanomolar range to maintain linearity.Instead of relying only on total ion chromatograms, they used extracted ion chromatograms (EICs) to improve signal-to-noise for the photocatalyst signal. Baseline subtraction was also applied to reduce noise and improve quantitation at low concentrations.Calibration curves and quality control samples were prepared and measured on the same day as the reaction samples, because LC-MS signal intensity can vary significantly from day to day.<h4>Why GC-MS was used later</h4>For demethylation studies, the group moved to GC-MS because the substrates and products were small and volatile enough for gas chromatography. GC-MS was better suited for rapidly screening many reaction conditions, including different photocatalysts, solvents, and oxidants.Instead of generating full calibration curves for every experiment, the team used an internal standard to streamline analysis and determine percent conversion based on relative response factors.<h4>Practical lessons from the project</h4>Repka highlights the importance of careful sample preparation, especially when working with very small quantities of photocatalysts. A high-quality microbalance, stable granite platform, antistatic tools, and aluminum weigh boats improved weighing accuracy and reproducibility.She also argues that mass spectrometry should be a standard part of photoredox method development because it can reveal minor products, support reaction optimization, and provide insights that may be difficult or impossible to obtain by NMR alone.<h4>Final perspective</h4>Beyond the chemistry, Repka emphasizes the importance of student training and mentoring. For her, research is not only about discovering new reactivity but also about giving students practical experience, helping them develop independence, and preparing them for future scientific careers.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Using LC-MS & GC-MS to Decode Photoredox Catalyst Stability

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Biomonitoring of toxicants like pesticides and PFAS in humans is essential for understanding how chemical exposure affects our health, yet chemical coverage is currently limited with targeted LC-MS approaches.Untargeted data acquisition, however, is emerging as a promising tool for broader chemical coverage and understanding the possible outcomes of chemical exposure on human health.In this webinar, our expert speaker will discuss how a new method with sequential acquisition of targeted and untargeted data can be used to analyze the exposome and the metabolome in a single LC-MS run, providing greater insights into the effects of chemical exposure.Attend this webinar to:<ul><li>Find out about the benefits of sequential targeted and untargeted data acquisition in a single LC-MS run</li><li>Understand how high sensitivity and broad chemical coverage can be achieved simultaneously for performing exposomic and metabolomic experiments</li><li>Discover a novel method that can be tailored for many different applications in the field of small molecule omics</li></ul><blockquote>Presenter: Dr. Vinicius Verri Hernandes (Postdoctoral Researcher at University of Vienna)</blockquote><blockquote>Vinicius obtained his degree in Chemistry followed by a Master in Analytical Chemistry in 2013 from the University of Campinas in Brazil, with focus on mass spectrometry. He later started a joint PhD program between the same university and the Centre for Excellence in Metabolomics in Bioanalysis in Madrid, Spain, working on multiplatform metabolomics on dried blood spots. From 2018 to 2022 he worked as a postdoctoral researcher at the European Research Academy (EURAC) in Bolzano, Italy, being responsible for targeted and untargeted data generation for 5000 samples from the CHRIS populational study. Currently at the University of Vienna, Vinicius focus his work on the combination of targeted and untargeted approaches for exposomics and metabolomics, as well as in the development of analytical workflows for exposure analysis on dried blood spots.</blockquote>

Technology Networks: Bridging Exposomics and Metabolomics in a Single LC-MS Run

Bridging Exposomics and Metabolomics in a Single LC-MS Run

How a new method with sequential acquisition of targeted and untargeted data can be used to analyze the exposome and the metabolome in a single LC-MS run.

We invite you to join a hybrid event on October 15th to explore the latest solutions and workflows for overcoming environmental and food contaminant testing challenges.This event will be broadcast live from the laboratory at Thermo Fisher Scientific Customer Solutions Center (CSC) in Paris. You can join in person at one of our many 'satellite' venues worldwide, where we will offer an extended agenda, or participate remotely through the live broadcast.During the live stream broadcast, you will meet our Customer Solution Centre specialist team and leading industry expert, John Quick, as they discuss innovative solutions to meet the ever-evolving testing demands. There will be plenty of time to ask these experts your questions.The following sessions will be streamed all over Europe and beyond:<ul><li>Detecting PFAS in drinking water to meet regulations across Europe – New automated sample preparation high-resolution MS solution to achieve parts per trillion detection limits. Aristide Ganci,&nbsp;Senior Product Application Specialist, Thermo Fisher Scientific; Valérie Thibert, Senior Product Application Specialist, Thermo Fisher Scientific; Bénédicte Gauriat, Senior Product Application Specialist, Thermo Fisher Scientific</li><li>New ready-to-go pesticide residue solution for faster, more confident results Zoe Julian, Product Marketing Specialist, Manager; Bénédicte Gauriat, Senior Product Application Specialist, Thermo Fisher Scientific; Julie Moriceau, Senior Product Application Specialist, Thermo Fisher Scientific</li><li>Low-level contaminant testing in environmental water matrices&nbsp;– High-Resolution Accurate Mass GC-MS approach to meet future regulations John Quick, Principal Scientist, ALS Environmental Ltd</li></ul>After the live stream concludes, 'satellite' locations offer a unique program onsite. Take this unique opportunity to connect with industry professionals, or join us to explore, network, and collaborate on your future projects. Scroll below to the venue of your choice to discover the individual agenda.Participation is free of charge, registration is required.For any questions about this event or regarding your registration, please email <a href="mailto:CMDEvents@thermofisher.com">CMDEvents@thermofisher.com</a>.<h3>Preliminary agenda</h3><h5>10:15 Live streaming of this event to ‘satellite’ locations and online audience</h5><h5>10:30 Introduction</h5><ul><li>Jeff Stubbs (Business Development Manager, Environment and Food Safety, Thermo Fisher Scientific)</li></ul><h5>10:55 Detecting PFAS in drinking water to meet regulations across Europe - New automated sample preparation high-resolution MS solution to achieve ppt detection limits</h5><ul><li>A Ganci, V Thibert and B Gauriat (Senior Product Application Specialists, Thermo Fisher Scientific)</li></ul><h5>11:35 Break</h5><h5>12:05 New ready-to-go pesticide residue solution for faster, more confident results</h5><ul><li>Z Julian, Product Marketing, B Gauriat, and J Moriceau (Senior Product Application Specialists Thermo Fisher Scientific)</li></ul><h5>12:45 Low-level contaminant testing in environmental water matrices – high-resolution GCMS approach to meet future regulations</h5><ul><li>John Quick (Principal Scientist, ALS Environmental Ltd)</li></ul><h5>13:15 Closing comments</h5><h5>13:30 End of live streaming</h5><div><div container-id="cq-colctrl-lt7" comp-id="B1"><div><div><div><div><div><div><blockquote><h3>How to attend?</h3>3 ways of attending:Customer Solution Centre (in-person event):&nbsp; Join the heart of the event at our Customer Solution Center in Paris, where the main agenda will be hosted and live-streamed worldwide to our ‘Satellite’ locations and our audience.Join a ‘Satellite’ location (in-person event):&nbsp; Our in-person ‘Satellite’ events will broadcast the main agenda live-streamed from our Customer Solution Center in Paris and supplement the day with local presentations, lab tours, or workshops. Take this opportunity to meet your Thermo Fisher representatives and industry experts from the region. Discover the individual agenda for your ‘Satellite’ location of interest.Online attendees (remote):&nbsp; Unable to travel to a ‘Satellite’ event? Not a problem! You can register to attend conveniently online from your desk or anywhere else through the live stream from our Customer Solutions Center in Paris.</blockquote></div></div></div></div></div></div></div></div>

Thermo Scientific: Solutions for environmental and food contaminant testing

Solutions for environmental and food contaminant testing

New and advancing workflows to meet and exceed European regulations.

Day two: Characterization and discovery<ul><li>Characterize and quantify calreticulin (CALR) arginylation using electron-activated top-down proteomics</li><li>Achieve in-depth profiling of metabolites and lipids in biological samples</li><li>Obtain accurate and reliable metabolite identification using electron-based fragmentation (EAD)</li><li>Detect trace amounts of pesticide residues in food by targeted and non-targeted, IDA and SWATH approaches</li><li>Enhance routine characterization of low-abundant sequence variant (SV) through fast and sensitive EAD-DDA</li></ul><h5>Agenda-at-a-glance</h5>Welcome and introduction to characterization and discoveryTop-down characterization of post-translational arginylation using electron activated dissociation (EAD)<ul><li>Learn about our top-down proteomics workflow for the characterization and quantification of calreticulin (CALR) arginylation. MS1 profile and MS2 fragmentation were characterized by EAD. Preferential fragmentation at the CALR N-terminals yielded sufficient c ions facilitating precise localization of the arginylation sites. Learn about:<ul><li>Arginylation regulates CALR degradation and ER function</li><li>EAD is efficient in characterizing N-terminal arginylation</li><li>The workflow sets up the foundation for unbiased top-down activity-based arginylation profiling</li></ul></li></ul>In-depth metabolomics and lipidomics analyses<ul><li>Routine measurement of metabolites and lipids has become a cornerstone of biomedical research. At the MPC, Helmholtz Munich, non-targeted analysis is based on the SCIEX ZenoTOF 7600 for accurate and in-depth analysis and annotation of metabolites and lipids from different biological specimens.<ul><li>Application of Zeno trap enabled non-targeted workflows to large scale metabolomics and lipidomics studies</li><li>Enhanced sensitivity enables deeper profiling of biological samples</li><li>Increased confidence in identifications through comprehensive structural elucidation using Electron activated dissociation (EAD)</li></ul></li></ul>MetID innovations in pharma: exploring new avenues for metabolite structure elucidationTime-of-flight mass spectrometry applications in food science: targeted and non-targeted approaches<ul><li>Time-of-flight mass spectrometry has become a key tool in analytical laboratories involved in food analyses. This instrumentation can be used for both targeted and non-targeted approaches. In this presentation, we will discuss:<ul><li>Overview of practical targeted and non-targeted approaches in food analysis</li><li>Application of a SWATH® approach to the analysis of pesticide residues in food</li><li>Application of IDA approaches in non-targeted food analyses</li></ul></li></ul>Reduce risk and accelerate cell line development with iCIEF-UV/MS technology<ul><li>Guide decisions during cell line development with the Intabio ZT system with more information on how process changes affect cell line and product stability. &nbsp;<ul><li>How to utilize icIEF-UV/MS to identify sequence variants</li><li>How this information can be used to create a mitigation strategy</li><li>What more information in less time means to groups throughout process development</li></ul></li></ul>Q&amp;A roundtable: A deeper look at high-resolution mass spectrometry<ul><li>Moderated by: ose Castro-Perez, Vice President of Product Management, SCIEX</li><li>Panelists include:<ul><li>Tom Lin, Instructor, Washington University in St. Louis</li><li>Michael Witting, Deputy Head Metabolomics and Proteomics Core and Executive Manager Metabolomics, Helmholtz Munich</li><li>Francisco José Díaz-Galiano, Postdoctoral researcher, University of Almería</li><li>Kirsten Nields, Principal Scientist, J&amp;J Innovative Medicines</li><li>Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEX</li></ul></li></ul><blockquote>Moderator: Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEXTatjana is a Senior Product Manager at SCIEX, overseeing accurate mass systems since 2021. She collaborates with R&amp;D, sales, and marketing teams to ensure effective project execution and portfolio management.Previously, Tatjana was a Product Manager at MOBILion Systems, leading the pre-commercialization of SLIM-based ion mobility instruments. She also has held roles at Thermo Fisher Scientific as a Sr. Vertical Marketing Specialist and Sr. Field Applications Scientist.Tatjana holds a B.A. in chemistry from Austin College and a Ph.D. in biomedical sciences and biochemistry from the University of North Texas Health Science Center.Jamie holds a B.Sc. in engineering physics from Queen’s University.Presenter: Tom Lin, Instructor, Washington University in St. LouisPresenter: Michael Witting, Deputy Head Metabolomics and Proteomics Core and Executive Manager Metabolomics, Helmholtz MunichPresenter: Francisco José Díaz-Galiano, Postdoctoral researcher, University of AlmeríaPresenter: Kirsten Nields, Principal Scientist, J&amp;J Innovative MedicinesPresenter: Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEX</blockquote>

SCIEX summit Day two: Characterization and discovery

Importance of LC-MS in food and environmental testing, pharmaceutical and biopharmaceutical discovery and development and biomedical and omics research.

CIEX summit Day two: Characterization and discovery

Day three: Quantitation and screening<ul><li>Enhanced sensitivity and robustness deliver stringent quantitation for veterinary drug testing for meat</li><li>See how intuitive, compliant-ready SCIEX OS software enables pharmacokinetic parameter monitoring with ease and reliability</li><li>Understand the challenges and best practices for oligoneucleotide analysis by LC-MS&nbsp;</li><li>Discover how exploiting fast acquisition rates can be used to identify and quantify over 2250 fatty-acids lipids</li><li>Hear about the accurate detection and quantitation of trace-lelvel PFAS compounds</li></ul><h5>Agenda-at-a-glance</h5>Welcome and introduction to quantitation and screening&nbsp;&nbsp;&nbsp;&nbsp;Veterinary drug analysis: SCIEX 7500 system vs 7500+ system<ul><li>Biochemie Lab shares their expertise in veterinary drug analysis. With over 25 years of experience and strict adherence to Commission Regulation (EU) No 37/2010, Biochemie Lab processes more than 100,000 samples annually, providing comprehensive food and environmental testing.<ul><li>Learn how Biochemie Lab conducts veterinary drug testing with specialized veterinarians, covering a wide range of analyses, including residual veterinary drugs, anabolic substances, and microbiological tests.&nbsp;</li><li>Discover how they overcome challenges in analyzing complex animal-origin matrices like meat and offal using the SCIEX QTRAP 7500 system.</li><li>Explore their proficiency testing, method validation results, and ask questions directly from leading experts in the field.</li></ul></li></ul>Maximizing analytical potential and navigating changes in the validated laboratory with SCIEX OS software<ul><li>Having the right information available at every stage of the process is vital to bringing safe and effective therapies to market.. SCIEX OS software is a vital component of this process, from discovery to development. See how:<ul><li>Your pharma lab workflows can benefit from SCIEX OS software</li><li>Central Admin Console enables comprehensive audit configurations for full traceability</li><li>Professional services are available to assist in migrating from legacy Analyst software and change control support plansis</li></ul></li></ul>Approaches for the bioanalytical analysis of oligonucleotide therapies<ul><li>Oligonucleotide therapeutics are on the rise in drug development and regulatory submissions. These drugs face challenges with bioanalytical extraction and analytical detection due to their chemical backbone or other stability-enhancing modifications.<ul><li>Learn about bioanalytical challenges and best practices for oligonucleotide analysis</li><li>Hear case studies in the quantitation of oligonucleotide therapies using different mass spectrometry platforms</li><li>Gain insights into improved method development for obtaining validated assays for regulatory evaluations using high-resolution mass spectrometry in early-stage development</li></ul></li></ul>Fast scanning quantitative lipidomics analysis using the SCIEX 7500+ system<ul><li>A panel of &gt;2250 lipids was used to demonstrate the power and capability of the SCIEX 7500+ system to sensitively identify and quantify lipids at the fatty acid level using a multiple reaction monitoring (MRM). HILIC-like chromatography provides class separation to mitigate inter-lipid class isobaric overlap, but means that the speed at which individual MRM transitions are measured is crucial to ensure good coverage and quantitative accuracy.<ul><li>The ~4X faster analytical speed of the SCIEX 7500+ system improves the quantitative performance of a large, broadly targeted lipid panel</li><li>The number of lipid molecular species quantified with %CV &lt; 20 increased 59% during a period of the highest</li><li>MRM concurrency (n = 925) compared to the fastest speed recommended on the SCIEX 7500 system The faster MRM acquisition rates improve peak shapes by increasing the number of data points across the peak and providing better resolution of near-eluting lipid isomers</li></ul></li></ul>From sample collection to data processing - PFAS program at State Hygienic Lab<ul><li>The State Hygienic Lab at University of Iowa had the PFAS program running for couple of years and is accredited and certified by the state, EPA, and UCMR5 program to test for PFAS in water samples. However, testing encompasses different environmental and biological matrices. This presentation will look at the program and focus on:<ul><li>Approaches from sample collection to data processing and reporting.</li><li>Testing for PFAS in water matrices (drinking, raw, well, surface, and groundwater) based on EPA 537.1 and 533 FAS methods.</li><li>New method for PFAS in biological samples.</li></ul></li></ul>Q&amp;A roundtable: &nbsp;quantitation and screening<ul><li>Moderated by: Ian Moore, Sr. Technical Product Manager, Nominal Mass, SCIEX</li><li>Panelists include:<ul><li>Roberto Riccio, Biochemie Lab</li><li>Shane Karnik, Senior Laboratory Director, Aliri Bioanalysis</li><li>Ebru Selen, Biologics Applications Scientist, SCIEX</li><li>Paul Baker, Senior Scientific Liason, Lipidomics and Metabolomics, SCIEX</li><li>Craig Butt, Senior Manager, Scientific Marketing, Applied Markets, SCIEX</li></ul></li></ul><blockquote>Moderator: Ian Moore, Sr. Technical Product Manager, Nominal Mass, SCIEXWith over ten years in product development, Ian is the Senior Technical Product Manager for nominal mass LC-MS/MS systems at SCIEX. He has also served as a Technical Product Manager and Global Technical Marketing Manager. Ian began his SCIEX career as an Application Scientist, focusing on biologics bioanalysis and DiscoveryQuant software validation.Before SCIEX, Ian was the Lead Analytical Scientist at Custom Biologics, overseeing LC-MS/MS method development and clinical trial analysis. He also served as a Senior Scientist at Affinium Pharmaceuticals, focusing on metabolic stability profiling and enzyme inhibition assays.Ian holds a postdoctoral position at McMaster University, specializing in antibiotic resistance and enzyme inhibitor screeningPresenter: Roberto Riccio (Biochemie Lab)Presenter: Shane Karnik (Senior Laboratory Director, Aliri Bioanalysis)Presenter: Ebru Selen (Biologics Applications Scientist, SCIEX)Presenter: Paul Baker (Senior Scientific Liason, Lipidomics and Metabolomics, SCIEX)Presenter: Craig Butt (Senior Manager, Scientific Marketing, Applied Markets, SCIEX)</blockquote>

SCIEX summit Day three: Quantitation and screening

Metabolomics, crucial for understanding biological interactions, faces challenges due to a lack of standardized protocols. Addressing this, the biocrates AbsoluteIDQ p400 HR kit offers a ready-to-use, quantitative protocol targeting 408 metabolites across 11 compound classes and includes calibration, internal standards, and quality controls.This webcast will describe how use of this targeted quantitative approach has now been validated for the Thermo Scientific Orbitrap Exploris family of mass spectrometers. Assessment using NIST SRM 1950 and human plasma samples demonstrates accuracy and reproducibility. Its applications span high-throughput analyses for large cohorts, drug discovery, and biomarker research, notably in neuroscience and cancer.You will learn:<ul><li>How targeted metabolomics can be applied on high resolution instruments</li><li>How standardization in metabolomics improves reproducibility across experiments and laboratories</li><li>How absolute quantification aids in data interpretation</li></ul><blockquote>Presenter: Stephen Dearth (Senior Applications Specialist, biocrates life sciences)Stephen Dearth is a senior applications specialist at biocrates life sciences ag, specializing in LC-MS-based targeted metabolomics. He has over 10 years of experience in the life sciences and has been with biocrates since 2017 after completing his PhD in chemistry with a focus on untargeted metabolomics and data analysis at the University of Tennessee.Moderator: Nikki Forrester (Freelance science, writer and editor)Nikki Forrester is a science journalist who covers biology, natural history, climate, and the culture of academic research. She earned a Ph.D. in ecology and evolutionary biology in 2019.</blockquote>

ThermoFisher Scientific: Quantitative metabolomics with validated standard protocols on high-resolution mass spectrometers

Quantitative metabolomics with validated standard protocols on high-resolution mass spectrometers

Targeted quantitative approach has now been validated for the Thermo Scientific Orbitrap Exploris family of mass spectrometers.

Quantitative metabolomics on HRMS

Food allergies are a public health and regulatory challenge, with an estimated 1–2% of adults affected by food allergies. Risk analysis and management of allergens within the food and beverage industry rely on accurate allergen analysis.High-performance liquid chromatography high-resolution mass spectrometry (HPLC-HRMS) could be the future of food allergen analysis.In this webinar, our expert speaker will discuss how HPLC-HRMS can detect a wide range of allergens in a single run, reducing the time and effort involved in food analysis. He’ll also highlight how integrating HPLC with HRMS approaches enhances accuracy and makes allergen detection more reliable, ensuring robust food safety and compliance with regulatory standards.<h4>Attend this webinar to:</h4><ul><li>Learn how to multiplex allergen detection &nbsp;</li><li>Improve the accuracy and speed of your food analysis &nbsp;</li><li>Discover a complete workflow including sample preparation and data analysis</li></ul><blockquote>Presenter: Prof. Jens Brockmeyer (Head of Department, Food Chemistry, University of Stuttgart, Germany)</blockquote>

Technology Networks: Moving Towards the Next Generation of Food Allergen Analysis

Moving Towards the Next Generation of Food Allergen Analysis

How HPLC-HRMS can detect a wide range of allergens in a single run, reducing the time and effort involved in food analysis.

Clinical blood analysis is crucial for monitoring health and diagnosing diseases, with blood-based biomarkers playing a key role.However, current detection methods often lack sufficient coverage. Mass spectrometry-based proteomics provides a robust solution for in-depth plasma proteome analysis, but precise sample preparation is essential.This webinar will guide you on maximizing plasma proteome coverage and optimizing sample preparation, whether using plasma or serum. Dr. König and Dr. Wendt will discuss developing an optimized, automated workflow for comprehensive plasma biomarker discovery, showcasing results from two complementary techniques: LC-MS and Olink.<h4>Attend this webinar to:</h4><ul><li>Discover how to efficiently prepare blood-derived samples, including plasma and serum</li><li>Explore how to implement a fully automatable, reproducible and standardized plasma workflow with scalable throughput</li><li>Achieve comprehensive plasma proteome characterization, addressing the dynamic range challenge for deeper insights</li><li>Learn to apply LC-MS-based proteomics combined with affinity-based methods to discover novel disease biomarkers</li></ul><blockquote>Presenter: Dr. Ann-Christine König (Proteomics Core Facility Manager, Helmholtz Zentrum Munich) Presenter:&nbsp;Dr. Fabian Wendt (Product and Application Manager, Mass Spectrometry, Tecan)</blockquote>

Technology Networks: Unlocking the Plasma Proteome: Advanced Profiling and Biomarker Discovery

Unlocking the Plasma Proteome: Advanced Profiling and Biomarker Discovery

Maximizing plasma proteome coverage and optimizing sample preparation, whether using plasma or serum.

Unlocking the Plasma Proteome: Advanced Profiling

Join us as we introduce our path to visualize molecules from tissue at single cell resolution using DESI MS imaging, with the commercial DESI XS ionization source coupled to Xevo G3 QToF Mass Spectrometer.This webinar will explain how to easily transition to high resolution MS Imaging with a traditional entry level commercial DESI MS imaging toolkit.<h4>Learn how to:</h4><ul><li>Understand High spatial resolution MS Imaging is now accessible to all labs and applications</li><li>Access single cell DESI Imaging with the combination of DESI XS and Xevo G3 QToF</li><li>Leverage both high sensitivity and high speed of acquisition with the Xevo G3 QToF without compromising MS Imaging data quality</li><li>Utilize HDI 1.8 Imaging software for microscope mode analysis for effective use of time in MS Imaging experiments</li><li>Obtain comprehensive and detailed spatial information with limited sample preparationAdd MSI analysis into a multi-modal imaging workflow with ease</li></ul><blockquote>Presenter:&nbsp;Dr Jamie Reilly (Postdoctoral Researcher, University of Galway)Dr. Jamie Reilly is a postdoctoral researcher in NUIG Galway. Prior to joining the CELLIMAGE project, Jamie undertook his PhD in Regenerative Medicine in REMEDI, where he used bioinformatics to analyze molecular pathways in cellular and animal models of autism spectrum disorder (ASD). Jamie attended Trinity College Dublin (TCD) for his undergraduate degree in Genetics, and has a Masters in Biotechnology from Queens University Belfast.Presenter:&nbsp;Dr. Mark Towers (Principal Scientist - MS Technology, Waters)Mark Towers gained his PhD from the University of Reading in Bioanalytical chemistry on the Development of applications and techniques for UV-Liquid-MALDI-MS. Mark joined Waters over 10 years ago into the Scientific operations team as a senior scientist, working on high resolution mass spectrometry and imaging.Mark’s primary area of focus has been surface analysis by desorption electrospray ionisation (DESI) with over 7 years experience and matrix assisted laser desorption ionisation for over 14 years, principally but not limited to tissue sections for the detection and distribution mapping of a wide range of molecules.Presenter: Dr. Joanne Ballantyne (Director, MS Imaging program, Waters Corporation)Joanne completed a PhD in Mass Spectrometry at UMIST in Manchester 2002, in collaboration with UK Ministry of Defence's Science and Technology Lab (Dstl). This was followed by a post-doc at NIH in Bethesda, USA, working in OMICS for the NIMH. After returning to the UK, and time as a MALDI applications chemist at Shimadzu Biotech, Joanne joined Waters Corporation in 2007 as a member of the OMICS Application team in Manchester and progressed to managing the team. Following this Joanne spent a number of years in Business Development and Field Marketing for discovery applications in the Northern European region. At the end of 2018 Joanne joined the Product Management group with responsibility for the SYNAPT and MS Imaging technologies. After gaining ILM7 accreditation in strategic leadership, Joanne is now Director of the MS Imaging program.</blockquote>

Waters Corporation: Towards Single Cell Imaging Session 1

Towards Single Cell Imaging Session 1

How to easily transition to high resolution MS Imaging with a traditional entry level commercial DESI MS imaging toolkit.

This session will provide a comprehensive introduction to the user interface, ensuring you can navigate with ease. Discover the various acquisition modes available and learn how to select the best one for your specific application.Additionally, we will explore the innovative Intelligent Reflex feature, highlighting its benefits in boosting your productivity.Whether you’re new to MassHunter or looking to refine your skills, this webinar is designed to enhance your understanding and efficiency.<blockquote>Presenter: Christian Hegmanns (Application Specialist LC/MS, Agilent Technologies, Waldbronn, Germany)Christian Hegmanns is a state-certified food chemist and works at Agilent as an LC/MS application specialist.With over 10 years of experience in the field of instrumental analysis, Christian develops customer-oriented solutions for a variety of applications with a focus on food analysis.</blockquote>

Agilent Technologies: MassHunter Masterclass Months

MassHunter Acquisition Q-TOF Part I – Basic Overview

Join us for an insightful webinar where we delve into the essentials of MassHunter Acquisition Q-TOF. 

This webinar will guide you through optimizing your Q-TOF for superior data quality by selecting the most suitable tune mode for your samples.We will delve into various MS acquisition modes, including MS only, All Ions (QRAI), Auto MS/MS (directed MS/MS; targeted MS/MS), and PRM mode.Learn how to fine-tune method settings to enhance your data quality and achieve the best analytical results. Whether you’re a seasoned user or new to QTOF, this session will provide valuable insights to elevate your mass spectrometry.<blockquote>Presenter: &nbsp;Stefan Schaebler (Application Specialist LC/MS, Agilent Technologies)Stefan completed his PhD in Biology, specializing in LC-MS-based lipidomics and metabolomics. Following his doctoral studies, he joined Agilent Technologies as an LC/MS Application Specialist. In this role, Stefan focuses on small molecule applications using LC-QTOF, with a strong emphasis on software tools and data interpretation.</blockquote>

MassHunter Acquisition Q-TOF Part II – Advance Settings

Join us for an in-depth exploration of advanced settings in MassHunter Acquisition for Q-TOF systems.

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