<div><p><strong>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</strong></p></div><div><div><div><div><h3><strong>What is Protein Purification?</strong></h3><p>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.</p><p>This process is especially vital in biotechnological areas inside immunology and microbiology. In <strong>immunology</strong>, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In <strong>microbiology</strong>, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</p></div></div></div></div><div><div><div><div><h3><strong>Types of Protein Purification Methods</strong></h3></div></div></div></div><div><p>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</p></div><div><div><div><div><h4><strong>Non-Chromatographic Approaches</strong></h4></div></div><div><p>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.</p><h5><strong>Precipitation Techniques</strong></h5><p>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:</p><ul><li><strong>Organic solvent precipitation</strong> involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li><strong>Isoelectric point precipitation </strong>exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li><strong>Nonionic polymer precipitation</strong> uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5><strong>Dialysis</strong></h5><p>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.</p><h5><strong>Immunocapture (Magnetic Bead-Based Purification</strong></h5><p>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:</p><ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul><p>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.</p><h5><strong>Centrifugation</strong></h5><p>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.</p><p><strong>Preparative Ultracentrifugation</strong><br>Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.</p><p><strong>Differential Centrifugation</strong><br>Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.</p><p><strong>Rate-zonal Gradient Centrifugation</strong><br>This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.</p><p><strong>Isopycnic Centrifugation (Equilibrium Centrifugation)</strong><br>Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</p></div></div></div><div><div><div><div><h4><strong>Chromatographic Approaches</strong></h4><p>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.</p><h5><strong>Ion Exchange Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526"><strong>Luna SCX </strong></a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540"><strong>Biozen WCX</strong></a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5><strong>Size Exclusion Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.</p><p>Phenomenex offers a range of <strong>Biozen columns for SEC</strong> separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534"><strong>Biozen dSEC-2</strong></a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"><strong> Biozen dSEC-7 </strong></a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.</p><p>For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533"><strong>Biozen SEC-2</strong></a><strong> </strong>and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532"><strong>Biozen SEC-3</strong></a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.</p><h5><strong>Reversed-Phase Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.</p><p>The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.</p><h5><strong>Hydroxyapatite Chromatography</strong></h5><p>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.</p><p>Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.</p><h5><strong>Hydrophobic Interaction Chromatography</strong></h5><p>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.</p><h5><strong>Affinity Chromatography</strong></h5><p>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.</p><p>This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</p></div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3><span><strong>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</strong></span></h3><p><span><strong>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.</strong></span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"></p><h4><span><strong>1. Improper Filter Handling</strong></span></h4><p><span>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.</span></p><ul><li><span><strong>Solution:</strong> Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</span></li></ul><h4><span><strong>2. Selecting an Inappropriate Pore Size</strong></span></h4><p><span>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.</span></p><ul><li><span><strong>Solution:</strong> A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</span></li></ul><h4><span><strong>3. Choosing the Wrong Membrane Material</strong></span></h4><p><span>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.</span></p><ul><li><span><strong>Solution:</strong> Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</span></li><li><span><strong>Pro Tip:</strong> For additional guidance, see our blog article <strong>"How to Choose the Right Membrane for your Syringe Filter"</strong>, which provides a detailed comparison of membrane materials and their recommended applications.</span></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"></p><h4><span><strong>4. Ignoring Filter Capacity</strong></span></h4><p><span>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.</span></p><ul><li><span><strong>Solution:</strong> When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</span></li></ul><h4><span><strong>5. Overlooking Sample Adsorption</strong></span></h4><p><span>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.</span></p><ul><li><span><strong>Solution:</strong> For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</span></li></ul><h4><span><strong>6. Failing to Optimize Filtration Performance</strong></span></h4><p><span>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.</span></p><ul><li><span><strong>Solution:</strong> If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</span></li></ul><h4><span><strong>Final Thoughts</strong></span></h4><p><span>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.</span></p><p><span>With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627"></p><p><span><strong>Happy filtering!</strong></span></p>

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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<p><strong>Are strong solvent effects after QuEChERS a challenge in your LC or LC/MS analysis? Agilent Feed Injection offers a solution. Learn more about this technology and application examples from food safety in this webinar.</strong></p>
<p>In recent years, QuEChERS has greatly simplified sample preparation in food safety analysis. However, downstream of QuEChERS, a strong solvent sample can cause strong solvent effects in LC and LC/MS analysis, leading to peak breakthrough and poor peak shapes, especially as the injection volume increases.</p>
<p>Agilent Feed Injection offers a solution to this challenge. The technique mediates the strong solvent effects by infusing the sample into the mobile phase stream. This allows you to achieve perfect peak shapes and easy peak integration without the need for additional sample preparation.</p>
<p>Join this webinar for a comprehensive introduction to Agilent Feed Injection from Dr. Juan Liang-Schenkelberg, Product Manager, and Dr. Edgar Naegele, Application Scientist, from Agilent. The presentation will be complemented by application examples from the field of food safety analysis.</p>
<p>Learning points:</p>
<ul>
<li><p><strong>Learn about common challenges in LC and LC/MS analysis following QuEChERS.</strong></p>
</li>
<li><p><strong>Learn about Agilent Feed Injection technology and how it helps overcome these challenges.</strong></p>
</li>
<li><p><strong>Discuss application examples from the field of food safety analysis with our expert speakers.</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Juan Liang-Schenkelberg (Strategic Product Manager at Agilent Technologies)</p>
</blockquote>
<blockquote>
<p>Juan Liang-Schenkelberg holds a PhD in biochemical engineering from the Hamburg University of Technology (TUHH) in Germany. Juan joined Agilent Technologies in 2016 as Product Manager of analytical HPLC. She is currently responsible for high-end UHPLC systems and for the food segment.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Edgar Naegele (Application Scientist at Agilent Technologies)</p>
</blockquote>
<blockquote>
<p>Edgar Naegele holds a PhD in organic chemistry from the Karlsruhe Institute of Technology (KIT) in Germany. Edgar joined Agilent Technologies in 2001 as an Application Scientist for LC and MS. Edgar is currently focused on the application of online LC and the new Agilent 1260 Infinity II Hybrid Multisampler in the pharmaceutical and food industries.</p>
</blockquote>


Less work, better peaks

Are strong solvent effects after QuEChERS a challenge in your LC or LC/MS analysis? Agilent Feed Injection offers a solution.

<p><strong>Welcome to Thermo Fisher Scientific’s Powering Productivity in Food and Environmental Testing webinar series!</strong></p>
<p><strong>Sample preparation for POPs analysis creates one of the biggest bottlenecks in the analytical workflow.  With such diverse matrices and compounds of potential interference, the sample prep process can be very manual and therefore prone to introducing errors- threatening productivity in food and environmental testing labs globally.</strong></p>
<p>In this presentation, our expert Chris Shevlin will introduce a new solution for automating this process, allowing you to extract your analyte of interest, whether a pesticide, dioxin or organic pollutant, in a way that is robust, reproducible and enables your lab to become more efficient by handling multiple samples and extractions at the same time.</p>
<blockquote>
<p><strong>Presenter:</strong> Chris Shevlin (Scientific and Educational Affairs Manager, Thermo Fisher Scientific)</p>
</blockquote>
<blockquote>
<p>Chris Shevlin is the Scientific and Educational Affairs Manager for Ion Chromatography and Automated Sample Preparation systems at Thermo Fisher Scientific. After graduating from the University of Buffalo, he started working in a pharmaceutical laboratory running HPLC, LC-MS and other chromatographic methods in a technical services and QC laboratory. After earning an MBA in 2004, Chris has worked in sales, product management and marketing with a few of the major analytical instrument manufacturers since then.</p>
</blockquote>
<blockquote>
<p>In 2015, Chris joined Thermo Scientific working with their chromatography and mass spectrometry systems and in 2019 moved into a product marketing role for Ion Chromatography and Automated Sample Preparation business unit. In his current role, he is partnering with scientists on new applications through collaborations, developing scientific and educational content, and working with new products and innovations.</p>
</blockquote>


Thermo Scientific: Recent Advances in Sample Prep for POPs

Recent Advances in Sample Prep for POPs

New solution for automating this process, allowing you to extract your analyte of interest, whether a pesticide, dioxin or organic pollutant.

<p><strong>In this session, we present an overview of the automated DEXTech Cleanup system, which offers a practical and affordable solution for detecting dioxins/furans, 209 PCB or PCNs, CPs, and PBDEs.</strong></p>
<p>The importance of enhancing laboratory setups to monitor persistent organic pollutants (POPs) is emphasized, particularly in light of incidents like the East Palestine, Ohio train accident. The DEXTech Cleanup system provides several advantages, including rapid turnaround time for results, suitability for processing high sample volumes, reduced manpower requirements, and affordability.</p>
<p>Three versions of the system will be presented: a standard unit without heating, a heated version, and an automated system capable of processing 15 samples. Data is presented for various POPs analysis methods, such as US-EPA Method 1668C for 209 PCBs, US-EPA Method 1613 for 2,3,7,8 substituted PCDD/Fs, and Commission Regulation (EU) 2017/644 for dioxin-like PCBs and indicator PCBs.</p>
<blockquote>
<p><strong>Presenter:</strong> Angelika Köpf (Head of Sales, LCTech, GmbH)</p>
</blockquote>


Gerstel: Automated Sample Cleanup with DEXTech: A Simple and Cost-Effective Solution for the Determination of POPs in a Wide Variety of Matrices

Automated Sample Cleanup with DEXTech: A Simple and Cost-Effective Solution for the Determination of POPs in a Wide Variety of Matrices

Overview of the automated DEXTech Cleanup system.

Automated Sample Cleanup with DEXTech

<p><strong>This session focuses on LCTech&#39;s specialized equipment designed to optimize each step of the dioxin workflow, ensuring efficient processing, high-quality data, and safety.</strong></p>
<p>Building on the success of the automated cleanup DEXTech system, LCTech has developed automated units for every stage of sample preparation, specifically tailored for PCDD/F, PCBs, PCNs, CPs, and PBDEs. These units prioritize user-friendly operation, robustness, high throughput, and prevention of cross-contamination.</p>
<p>The X-TRACTION unit is designed for pressurized fluid extraction (PFE) and offers exceptional ease of handling and cost efficiency while maintaining robust performance.</p>
<blockquote>
<p><strong>Presenter:</strong> Angelika Köpf (Head of Sales, LCTech, GmbH)</p>
</blockquote>


Gerstel: Introducing an Automated Solution to Enhance Efficiency and Streamline the Entire Dioxin Workflow

Introducing an Automated Solution to Enhance Efficiency and Streamline the Entire Dioxin Workflow

LCTech's specialized equipment designed to optimize each step of the dioxin workflow, ensuring efficient processing, high-quality data, and safety.

Introducing an Automated Solution to Enhance Efficiency

<p><strong>Immunopeptidomics is a field that combines proteomics and immunology to study the complex repertoire of peptides presented by major histocompatibility complex (MHC) molecules on the cell surface.</strong></p>
<p>These MHC-bound peptides, known as immunopeptidomes, play crucial roles in immune recognition and activation, influencing various physiological and pathological processes such as immune surveillance, infectious diseases, autoimmunity, and cancer.</p>
<p><strong>Join our webinar to explore recent advancements, challenges, and applications in immunopeptidomics. Discover how EvosepOne accelerates discoveries, and support the understanding of immune responses and diseases.</strong></p>
<h4><strong>IMMUNOPEPTIDOMICS ON THE EVOSEP ONE – NEW LEVELS OF SAMPLE THROUGHPUT AND PRODUCTIVITY</strong></h4>
<p>Immunopeptidomics is the study of peptide antigens that are bound to and presented on the cell surface by molecules encoded by the Major Histocompatibility, a highly polymorphic region of the genome that encode molecules involved in immune responses. These peptides are derived from both intracellular and extracellular degradation of the parental proteins, generating a highly complex repertoire of peptide ligands, including peptides derived from pathogens, tissue specific and cancer specific antigens when relevant.  In humans, human leukocyte antigen (HLA) class I and class II molecules are of particular interest for immunopeptidomic analysis. These molecules are encoded by several loci and constitute thousands different alleles that vary between individuals. Thus, immunopeptidomes are highly individualized and almost impossible to accurately predict.  This predicates the empirical measurement of the complexity and diversity of immunopeptidomes using mass spectrometry. Due to the relatively small amounts of material available for analysis the use of nanoflow LC-MS for immunopeptidome analysis is critical. Here I explore the implementation of the high throughput EvoSEP one platform couples to a Bruker timsTOF Pro 2 for robust analysis of immunopeptidomes across a variety of tissues and cell lines. Using the Whisper 20SPD method data quality and depth is maintained whilst sample carryover is negligible allowing more productive use of instrument time; I highlight significant improvements in throughput with over 1500 immunopeptidomes characterised in just 3 months.</p>
<blockquote>
<p><strong>Presenter:</strong> Anthony Purcell (Professor, Purcel Lab, Monsah University)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Nicolai Bache (Head of Applications at Evosep)</p>
</blockquote>
<h4><strong>MAPPS: High throughput immunopeptidomics for the assessment of the immunogenic potential of protein biotherapeutics</strong></h4>
<p>Biologics, mostly monoclonal antibodies and recombinant proteins, are a class of drugs used to treat some particularly difficult conditions. These proteins, when recognized as non-self by the immune system, can elicit unwanted immunogenicity. One of the first steps in this cascade is the presentation of fragments of this protein by MHC complexes on antigen presenting cells, which might ultimately lead to generation of anti-drug antibodies (ADAs). These ADAs can lower the therapeutic efficiency and can lead to severe immunogenic effects. To bring safe biotherapeutics to the market it is critical to thoroughly assess the immunogenic profile of a biotherapeutic. MAPPS (MHC associated peptides proteomics) assays are the method of choice to define all potential immunogenic regions of a protein. ImmuneSpec, an advanced immunopeptidomics platform, offers MAPPS as a service. Here we show how the combination of high-throughput MHC peptide purification, fast LC using EvoSep, and high sensitive MS addresses these needs.</p>
<blockquote>
<p><strong>Presenter:</strong> Kurt Boonen (Assistant Professor, Deparment of biomedical sciences, University of Antwerp)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Nicolai Bache (Head of Applications at Evosep)</p>
</blockquote>


Evosep: ADVANCING IMMUNOPEPTIDOMICS: LEVERAGING EVOSEP ONE FOR HIGH-THROUGHPUT ANALYSIS AND DISCOVERY

ADVANCING IMMUNOPEPTIDOMICS: LEVERAGING EVOSEP ONE FOR HIGH-THROUGHPUT ANALYSIS AND DISCOVERY

Recent advancements, challenges, and applications in immunopeptidomics.

Immunopeptidomics: HIGH-THROUGHPUT ANALYSIS AND DISCOVERY

<p><strong>The identification of extractables, leachables and chemical migration is a challenge for manufacturers and suppliers of packaging materials, medical devices and food contact materials. E&amp;L analytics is already complex, but it is compounded by legislative and regulatory efforts to recycle plastics. Analytical methods need to screen for not only the known components but also identify degradants and other unexpected compounds. Manufacturers, producers and retailers face the challenge of keeping up with evolving requirements and developing and adapting strategies to reduce plastic waste.</strong></p>
<p>Thus, ways are being explored to incorporate more recycled plastics into products and packaging while maintaining excellent safety, quality and performance characteristics. As they innovate and combine new materials, companies must demonstrate safety and confirm compliance before bringing their products to market. This again increases the demand and importance of highly effective analytics.</p>
<p>Register now for this virtual event and share knowledge and challenges with peers and E&amp;L experts.</p>
<p>Who Should Attend?</p>
<p>The webinars are focused on the needs of scientists working in the area of Extractables &amp; Leachables and Food Contact Materials. During these webinars, attendees will learn:</p>
<ul>
<li><p><strong>Upcoming and new regulations in the field of food, pharma, biopharma and medical devices</strong></p>
</li>
<li><p><strong>Targeted and non-targeted screening workflows by using Waters_Connect</strong></p>
</li>
<li><p><strong>Characterization and structural elucidation of unknowns</strong></p>
</li>
<li><p><strong>Choice of chromatographic techniques, extraction methods and analytical standards &amp; reagents</strong></p>
</li>
</ul>
<h3>EPISODE 3 - Virtual European Workshop on Extractables &amp; Leachables for Medical Devices</h3>
<p>Agenda:</p>
<h4><strong>The role of chemical characterization in the evaluation of the biological safety of medical devices</strong></h4>
<ul>
<li>Dr. Séverine Colas, Chief Scientific Officer, TentaConsult Pharma &amp; Med GmbH, Germany</li>
</ul>
<h4><strong>Chemical Characterisation of Dressings: Extraction and Analytical Challenges</strong></h4>
<ul>
<li>Pauline Kisseleff, Analytical Expert, URGO Recherche Innovation et Développement, France</li>
</ul>
<h4><strong>Leachables from Pharma and Medical device materials, a non-target challenging analysis</strong></h4>
<ul>
<li>Prof. Cristina Nerin, University of Zaragossa, Spain</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Séverine Colas (Chief Scientific Officer, TentaConsult Pharma &amp; Med GmbH)</p>
</blockquote>
<blockquote>
<p>Séverine Colas is a PhD and a toxicologist (ERT) with more than fifteen years of experience in regulatory toxicology. She has always worked in services for the safety and regulatory compliance of products, initially with pharmaceuticals, then with chemicals and for more than 10 years now with medical devices. She has joined the TentaConsult team to provide to the client her strong expertise on biological evaluation of medical devices (MDR, ISO 10993-1 and related guidelines) and support them to establish the best strategy for the compliance of their medical device throughout their life cycle. If testing is needed to complete the evaluation, she also provides the client with her experience in conducting biocompatibility studies to find the suitable laboratory and to establish the more pertinent study design. As Chief Scientific Officer and Sales Director in TentaConsult, she is the preferred contact of the client to provide the best offer for the need.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Pauline Kisseleff (Analytical Expert, URGO Recherche Innovation et Développement)</p>
</blockquote>
<blockquote>
<p>Pauline Kisseleff has a Master degree in analytical chemistry and received her PhD on container-content interactions applied to cosmetic products in 2020. She is currently in charge of extractables and leachables studies at URGO Research, Innovation and Development, where she works on several medical devices such as technological dressings</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Cristina Nerin (Professor, Department of Analytical Chemistry, University of Zaragoza)</p>
</blockquote>
<blockquote>
<p>Full Professor at the University of Zaragoza (Spain), PhD in Analytical Chemistry, Master in Science (Analytical Chemistry) and Degree in Chemistry at the University of Zaragoza (Spain). Director of the Research Group GUIA at the University of Zaragoza and Director of the Master in Environmental Engineering at the University of Zaragoza from 1990 to 2012. Author of more than 395 Scientific Publications included in the Citation Index, Director of 38 PhD Thesis (5 more are in process). Principal Investigator of more than 240 Research Projects in both Competitive calls and also R&amp;D&amp;I with Industry Inventor in six International Patents dealing with active packaging and in one International Patent on Intelligent Packaging. All of them are in exploitation (market). Six Research Awards received. Participant in more than 140 International Conferences and Professor of several International Courses n Europe, Asia and South-America.</p>
</blockquote>


Waters Corporation: Virtual European Workshop on Extractables & Leachables for Medical Devices

Virtual European Workshop on Extractables & Leachables for Medical Devices

Register now for this virtual event and share knowledge and challenges with peers and E&L experts.

Virtual European Workshop on Extractables & Leachables

<p><strong>In this session, explore the operation and latest developments of automated SPME for VOCs and SVOCs by headspace, direct immersion, and derivatization techniques.</strong></p>
<p>Furthermore, discover how SPME has key applications for food, water, environmental and forensic samples for use in laboratories for GC-MS and LC-MS and direct MS analysis.</p>
<blockquote>
<p><strong>Presenter:</strong> Hans-Joachim Huebschmann (Consultant, VP Sample Preparation, CTC Analytics)</p>
</blockquote>


SelectScience: Solid phase microextraction – How does it work for my samples?

Solid phase microextraction – How does it work for my samples?

Explore the operation and latest developments of automated SPME for VOCs and SVOCs.

Solid phase microextraction–How does it work for my samples?

<p>Key Learning Points:</p>
<ul>
<li><p><strong>Learn different sample preparation techniques, including solid support liquid extraction, Quechers and SPE</strong></p>
</li>
<li><p><strong>How to develop a SPE method based on sample matrix and compound properties</strong></p>
</li>
<li><p><strong>The challenge and workflow in the food Industry</strong></p>
</li>
</ul>
<p>Who Should Attend:</p>
<ul>
<li>Any beginner or intermediate scientists looking to learn more about sample preparation</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Grace Guo (Senior Technical Specialist, Phenomenex)</p>
</blockquote>
<blockquote>
<p>Grace graduated from the Johns Hopkins University with a Master’s degree in Material Science and Engineering in 2014. In her graduate study, she developed a low-voltage organic phototransistor with an organic small molecule. After her graduation she moved from the east coast to west coast because she loves the all year long sunshine in South California.</p>
</blockquote>
<blockquote>
<p>She spent 3 years in a start-up company as Device Scientist doing single walled carbon nanotube separation and making thin film transistor with them. Most of her experience with Flash system are gained from there. In 2017, she joined Phenomenex as a Technical Specialist supporting globally, especially the Asia-Pacific area. Bilingual in Chinese and English makes it easy for her to communicate with customers in both US and China.</p>
</blockquote>
<blockquote>
<p>At Phenomenex she has more opportunity to have hands-on experience in HPLC, GC and SPE. Discussing the crazy troubleshooting cases with other technical members improves her chromatographic skills every day!</p>
</blockquote>


Phenomenex: Sample Prep for Crude Extraction Removal & SPE Method and Development

Sample Prep for Crude Extraction Removal & SPE Method and Development

Learn different sample preparation techniques, including solid support liquid extraction, Quechers and SPE.

Sample Prep for Crude Extraction Removal & SPE Method

<p><strong>Amino Acid Analysis is required in many applications related to foods, dietary supplements and animal feeds and is a fundamental tool for monitoring product QC and compliance with nutritional labeling.</strong></p>
<p>AOAC International has recently published a standardized method (AOAC Official Method 2018.06) for the analysis of total amino acids in infant formulas and adult nutritionals by UHPLC-UV.  For some samples, to obtain a full amino acid profile, the samples must first be hydrolyzed. Due to the varied properties of amino acids and diversity of sample matrices, hydrolysis, separation and detection represents an analytical challenge. Learn more about effective hydrolysis, separation and detection of amino acids in dietary supplements, plant-based meats, and pet food during a 3-part webinar series with CEM and Waters.</p>
<blockquote>
<p><strong>Presenter:</strong> Alicia Douglas Stell, Ph.D. (Market Development Manager, Applied Markets, CEM Corporation)</p>
</blockquote>
<blockquote>
<p>Dr. Alicia Douglas Stell has worked with CEM for the past 15 years, joining after completing her PhD in Analytical Chemistry. Her extensive background in chromatographic analysis and her mechanical expertise have been integral in the development of both instrumentation and novel applications. In her role as Market Development Manager, she is continuously exploring new applications and markets for innovative technology, focused around sample preparation.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Benedict Liu (Product Specialist, Organic Solutions, CEM Corporation)</p>
</blockquote>
<blockquote>
<p>Benedict Liu graduated from UNC Chapel Hill in 2018 with a bachelor&#39;s degree in chemistry and mathematics. He joined CEM soon after and has been with the company for 5 years serving as an applications chemist and product specialist for the Molecular Sample Preparation division. In his time at CEM, Ben has worked to develop solutions for the extraction of organic pollutants in soils and product, microwave amino acid hydrolysis protocols, and automated extraction methods for PFAS in solid and semi-solid matrices.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Paula Phenix (Principal Product Marketing Manager, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Paula is a Principal Product Marketing Manager at Waters with a focus on products for amino acid and glycan analysis. Prior to her current role in the Chemistry and Lab Automation Group, she was the Product Marketing Manager for Specialty LC and SFC Systems in the Instruments group. Before joining Waters, she held product management, marketing and sales roles for several companies (Life Technologies, Promega, MilliporeSigma) in the molecular biology, immunology, cell culture and single-use bioprocessing areas. Paula graduated from the University of Delaware with a Bachelor’s degree in Biology and from Johns Hopkins University with a Master’s degree in Business.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Ravali Alagandula, Ph.D. (Chemistry Market Development Manager, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Ravali is a Chemistry Market Development Manager at Waters with focus on all chemistry consumables and lab automation products. She holds a Ph. D. in Analytical Chemistry from Cleveland State University. She started her career as an LC applications scientist at Restek, developing LC-MS/MS and sample preparation methods in clinical and life science markets. Most recently, Ravali was a Chemistry Account Manager at Waters supporting key accounts and customers to promote chemistry consumable business in all market areas.</p>
</blockquote>


Waters Corporation: Easy & Efficient Amino Acid Sample Prep followed by Accurate Detection - a look at Dietary Supplements

Easy & Efficient Amino Acid Sample Prep followed by Accurate Detection - a look at Dietary Supplements

Learn more about effective hydrolysis, separation and detection of amino acids in dietary supplements, plant-based meats, and pet food.

A look at Dietary Supplements

<h5><strong>The Problem</strong></h5><p>Buffer exchange is a critical step to developing optimal biologics, vectors, and payloads. Formulation development is a challenge to perform at scale, but critical for efficient drug development. Finding out early how leads perform in different buffer conditions saves valuable time. Performing buffer exchange and concentration for formulation development with conventional exchange methods is especially challenging when you have lots of conditions or samples to screen. Throw in unexpected variations like inconsistent starting sample volumes or concentrations, and you’ll be looking for a better solution.</p><h5><strong>The Solution</strong></h5><p>Big Tuna is the automated, high-throughput buffer exchange and concentration instrument designed for these situations. Using pressure based ultra-filtration/diafiltration (UF/DF) technology coupled with non-contact individual sample volume measurement, Big Tuna will deliver precise buffer exchange and concentration targets without putting a dent in your sample recovery. Researchers can now rely on Big Tuna to automatically normalize below target initial sample volumes to give consistent results even when sample preps are not. With the ability to parallel process up to 24 samples in a volume range between 0.5 – 48 mL or up to 96 samples in a 100 – 400 µL volume range Big Tuna can help with screening, formulation, quality control, and analytical prep workflows. Programmable settings, including the optional orbital mixing, can be used with default settings, or customized to your unique biologic and gene therapy samples.</p><h5><strong>The Proof</strong></h5><p>Join us to see firsthand data results demonstrating the power and flexibility of Big Tuna for biologics, nucleic acids, LNPs, and AAVs. Final exchange and concentration values, along with analytic characterization, will be shown under different conditions.</p><blockquote><p><strong>Presenter:&nbsp;</strong>Del Ray Jackson (Market Manager, Buffer Exchange &amp; Automation, Unchained Labs)</p><p><strong>Moderator: </strong>Alex Shephard (Market Manager, Cell Therapy at Unchained Labs)</p><p>Alex Shephard is the Market Manager for Cell Therapy at Unchained Labs. She has many years of experience at the bench and as a field application scientist characterizing exosomes and viruses, using techniques including NTA, DLS, nanoflow cytometry and interferometry. Alex completed a Ph.D. in Molecular Biology at Bristol University, UK, and held a postdoc position in the Tumour Microenvironment Group at Cardiff University, UK.</p></blockquote>

Unchained Labs: Get flippin' flexible biomolecule sample prep with powered-up Big Tuna

Get flippin' flexible biomolecule sample prep with powered-up Big Tuna

Join us to see firsthand data results demonstrating the power and flexibility of Big Tuna for biologics, nucleic acids, LNPs, and AAVs.

LCMS webinars focusing on Sample prep - page 25