<div>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</div><div><div><div><div><h3>What is Protein Purification?</h3>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.This process is especially vital in biotechnological areas inside immunology and microbiology. In immunology, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In microbiology, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</div></div></div></div><div><div><div><div><h3>Types of Protein Purification Methods</h3></div></div></div></div><div>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</div><div><div><div><div><h4>Non-Chromatographic Approaches</h4></div></div><div>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.<h5>Precipitation Techniques</h5>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:<ul><li>Organic solvent precipitation involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li>Isoelectric point precipitation exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li>Nonionic polymer precipitation uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5>Dialysis</h5>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.<h5>Immunocapture (Magnetic Bead-Based Purification</h5>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:<ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.<h5>Centrifugation</h5>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.Preparative Ultracentrifugation Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.Differential Centrifugation Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.Rate-zonal Gradient Centrifugation This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.Isopycnic Centrifugation (Equilibrium Centrifugation) Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</div></div></div><div><div><div><div><h4>Chromatographic Approaches</h4>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.<h5>Ion Exchange Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526">Luna SCX </a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540">Biozen WCX</a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5>Size Exclusion Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.Phenomenex offers a range of Biozen columns for SEC separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534">Biozen dSEC-2</a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"> Biozen dSEC-7 </a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533">Biozen SEC-2</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532">Biozen SEC-3</a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.<h5>Reversed-Phase Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.<h5>Hydroxyapatite Chromatography</h5>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.<h5>Hydrophobic Interaction Chromatography</h5>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.<h5>Affinity Chromatography</h5>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

Article

Product

Application

Academy

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

DataApex

Agilent Technologies

Waters Corporation

Is your lab ready to begin its digital transformation?
Using IoT sensor technologies, AI modeling, and advanced analytics, your digital lab can safely capture data, create business intelligence,and implement actioned programs. Connect your lab digitally by watching this episode of The Lab Central.
<blockquote>
Presenter: Kamesh Janakiraman (Product Specialist - Lab Informatics, Agilent Technologies)
</blockquote>

Agilent Technologies: The Digital Lab Connection

The Digital Lab Connection

Using IoT sensor technologies, AI modeling, and advanced analytics, your digital lab can safely capture data.

Data integrity is strengthened through a detailed understanding of your data. This includes mapping your data flow, which necessitates questions such as:
<ul>
<li>How is data created?
</li>
<li>Where is data stored?
</li>
<li>What other data is relevant?
</li>
<li>How and where is data used within your business process?
</li>
<li>Where could risk exist?
</li>
</ul>
Understanding your data workflow allows you to focus in on the most critical data so you can identify potential data integrity risks and vulnerabilities. Facilitating a risk-based approach to process design, data integrity controls, workflow validation, and computerized system validation (CSV) enables you to confidently identify areas to monitor as you strive to ensure quality.
Please join us for a lively discussion as we explore this topic. As always, don’t forget to bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.
<blockquote>
Presenter: Neil Lander (Principal Product Manager - Informatics Group, Waters Corporation)
</blockquote>
<blockquote>
Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager in Waters in the Informatics group. Neil provides internal support for Empower CDS software and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters Corporation, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.
</blockquote>
<blockquote>
Presenter: Fiona O&#39;Leary (Empower Informatics Consultant, Waters Corp.)
</blockquote>
<blockquote>
Fiona is an experienced Empower Software and Data Integrity professional working with the EU Professional Services Team. She has ~18 years of experience with Waters, beginning with Technical Support &amp; Empower Training, moving to Empower Consultancy and Workflow Optimization in more recent years. Her focus now is on building Data Integrity into the Lab workflow, Custom Field automation and Electronic Data Review / Reporting optimization.
</blockquote>
<blockquote>
Prior to joining Waters, Fiona spent some years in the GxP Pharmaceutical Manufacturing world, setting the scene for her currently goal of helping organizations achieve their scientific and operational objectives efficiently and within compliance guidelines. She graduated from Dublin City University in 2001 with a BSc Hons. Analytical Science.
</blockquote>
<blockquote>
Presenter: Tracy Hibbs (Principal Consulting Product and Marketing Manager, Waters Corp.)
</blockquote>
<blockquote>
Tracy has worked for Waters Corporation since 1999 and has held a variety of positions including Technical Trainer, Business Impact Consultant, Training Manager for Informatics and Data Integrity, and currently, Principal Consulting Marketing Manager focusing on Informatics solutions. Tracy provides support for Waters Empower Software Suite and is concentrated on developing laboratory data management software solutions that help organizations achieve their scientific and operational objectives in regulated environments. Prior to joining Waters, Tracy was a Research Scientist where she gained experience in chromatography and the associated data systems.​
</blockquote>

Waters: Empower Tips: Ask Me Anything About Data Flow and Data Process Mapping with Data Governance in Mind

Empower Tips: Ask Me Anything About Data Flow and Data Process Mapping with Data Governance in Mind

Empower: Data Flow, Data Process Mapping and Data Governance

How about a 20 minute coffee break along with a bite of <a href="https://lcms.cz/products/627">Empower</a>? Here you go!
In this session we demonstrate how to create and use Customer Fields to generate workflows in Empower
A Custom Field is a user defined field (or column) of information in a table. Creating Custom Fields gives you the ability to create tailored sample identifiers and calculations beyond what Empower provides by default. During this series, we will explore the various types of Custom Fields that can be created and provide some practical examples.
Learn more and register!
<blockquote>
Presenter: Carsten Fischer (Informatics Product Marketing, EMEA, Waters Corporation)
</blockquote>

Waters - Informatics series #12 Adjust Empower very flexible to your specific needs

Adjust Empower very flexible to your specific needs

In this session we demonstrate how to create and use Customer Fields to generate workflows in Empower.

Learn incredible tricks and tips for maximizing your experience with <a href="https://gcms.cz/products/305">Clarity Chromatography Software</a>. Dive into advanced functionalities and discover how to elevate your workflows.
We will closely look at Sequences and Calibrations.

Clarity Tips & Tricks Webinar

Learn incredible tricks and tips for maximizing your experience with Clarity Chromatography Software

DataApex Clarity software Tips & Tricks Webinar

How about a 20 minute coffee break along with a bite of Empower? Here you go!
Avoid manual weight transcriptions to your Empower Sample Set
Many roads lead to Rome - In this session we show different options to transfer sample weight to Empower.
Two completely different options will be highlighted: The Sample Weight Importer toolkit app versus a test execution template in Empower LMS.
The sample (and standard) weights play an important role in the quantitative chromatographic analysis. In this session you will get examples of how to eliminate the manual transcription of sample weights by using a direct read from your balance.
Learn more and register!
<blockquote>
Presenter:: Ralf Schröder (Informatics Product Marketing Europe, Waters GmbH)
</blockquote>
<blockquote>
Presenter: Carsten Fischer (Informatics Product Marketing, EMEA, Waters GmbH)
</blockquote>

Waters Corporation: Informatics series #13 Sample Weights in Empower

Informatics series #13 Sample Weights in Empower

In this session we show different options to transfer sample weight to Empower.

Sample Weights in Empower

Agilent and METTLER TOLEDO have jointly developed an integrated workflow solution that addresses the most pressing root causes for laboratory failure investigations and rework, while supporting the current processes in the laboratory.
The integrated solution between Mettler Toledo LabX Balance Software &amp; Agilent OpenLab CDS, addresses the challenges like:
<ul>
<li>Manual data entries of weight</li>
<li>Potential errors due to manual calculations</li>
</ul>
It also enhances productivity in overall Chromatography workflow.
Register here for our webinar and learn more on how our solution can increase the weighing accuracy, automatically transfer the weighing data to the CDS, and eliminate manual transcription errors
<blockquote>
Presenter: Kevin Kölliker (Lab Informatics Application Engineer, Agilent Technologies)
</blockquote>
<blockquote>
Kevin Kölliker is a Lab Informatics Application Engineer for Agilent Technologies. He studied Chemistry and Business informatics, and he worked as a Lab Technician, and as a Field Service Engineer before joining Agilent. Combined he has more than 10 Years of Experience in the analytical laboratory and therefore met already multiple different workflows. He works together with the Lab Informatics Product Specialists to demonstrate the Power of OpenLab, and to customize it to meet the needs of our Customers.
</blockquote>
<blockquote>
Presenter: Andrea Brütsch (Global Product Manager, Mettler Toledo)
</blockquote>
<blockquote>
Andrea Brütsch is a Global Product Manager at METTLER TOLEDO. He holds a master&#39;s degree in Chemistry and Business Studies from the University of Zurich. After various experiences at METTLER TOLEDO, Andrea wrote a study on the trends in Laboratory Automation. Currently, he is responsible for the area of lab automation in the Laboratory Weighing business.
</blockquote>

Agilent: Error-Free Sample Preparation Workflows

Error-Free Sample Preparation Workflows

Agilent and METTLER TOLEDO have jointly developed an integrated workflow that addresses the most pressing root causes for laboratory failure investigations.

The development momentum for RNA modalities has accelerated with the recent approvals of mRNA-based vaccines and RNA(CRISPR)-based gene therapies. These approvals have highlighted the need for the development of robust analytical methods for monitoring the relevant critical quality attributes (CQAs) of RNA molecules to ensure proper control of manufacturing processes and product quality. Oligonucleotide mapping via liquid chromatography coupled to mass spectrometry (LC-MS) has now been established as an effective tool for RNA CQA assessment, however, the limited tool sets for efficient acquisition and processing of the LC-MS data from an RNA digest have presented a time-consuming challenge to the innovators bringing these early molecules to market.
In this webinar, we present a workflow for RNA CQA analysis developed on the waters_connect informatics platform. Data acquired on this compliant-ready platform was analyzed using the INTACT Mass application as the engine for data processing and producing RNA fragment assignments, assisted by two external microApps: mRNA Cleaver (to generate in silico fragments for targeted search), and Coverage Viewer (to visualize digestion data coverage). We will demonstrate these tools with application to CQA analysis of both sgRNA (ID and Structural Fidelity) and mRNA (ID, Structural Fidelity, 5’Capping, 3’ PolyA Tail A) molecules. 
What you will learn from this presentation:
<ul>
<li>A workflow for acquisition and data analysis from RNA digestion fragment mapping studies.</li>
<li>Application of this workflow to sgRNA and mRNA CQAs.</li>
<li>Discussion of the challenges and opportunities deploying these methods for routine analysis, including the use of the BioAccord LC-MS System and waters_connect informatics to make MS based RNA analysis accessible to non-MS experts.</li>
</ul>
Who should attend?
<ul>
<li>Scientists, Technicians and Managers, who are charged with analyzing therapeutic RNA samples.</li>
<li>Others from pharmaceutical and contract organizations looking towards developing and commercializing these molecules, who want to better understand the analytical challenges of producing high quality products and processes.</li>
</ul>
<blockquote>
Presenter: Ying Qing Yu (Biopharmaceutical Program Science Team Leader, Waters Corporation)
</blockquote>
<blockquote>
Ying Qing is the Biopharmaceutical Program Science Team Leader at Waters Corporation. She joined Waters Corporation in 2001, shortly after she received her Ph.D. from the Analytical Chemistry Department at Purdue University. Her area of expertise is on analytical method development for various Biotherapeutics using UPLC/QTOF MS platform, including sample preparations, LC-MS optimization, and development of application specific software tools. Recently, her team has been taking on the challenges of developing LC-MS solutions for nucleic acid characterization.
</blockquote>

Waters: LC-MS for RNA Therapeutics to Ensure Product Quality and Process Consistency

LC-MS for RNA Therapeutics to Ensure Product Quality and Process Consistency

Workflow for RNA CQA analysis developed on the Waters_connect informatics platform.

LC-MS for RNA Therapeutics Product Quality

Minimize risk, streamline workflows and improve the reliability of your analytical measurements.
How do the Alliance iS and Empower work together to reduce common human errors?
Empower 3.8.0 offers unique features that work specifically with Waters Alliance iS HPLC System and can reduce common made human errors.
Learn how to identify potential errors earlier, reduce the number of retests and failure investigations and register!
<blockquote>
Presenter: Arjan Timmerman (Principal Pharma Market Development Manager Pharmaceuticals, Compliance and Impurities)
</blockquote>
<blockquote>
Presenter: Andrea Gheduzzi (Principal LC Market Development Manager, EMEA)
</blockquote>
<blockquote>
Presenter: Carsten Fischer (Informatics Product Marketing, EMEA)
</blockquote>

Waters Corporation: Reducing Human Errors with Empower 3.8.0

Reducing Human Errors with Empower 3.8.0

Empower 3.8.0 offers unique features that work specifically with Waters Alliance iS HPLC System and can reduce common made human errors.

In this session we demonstrate how to create and use Customer Fields to generate workflows in Empower

Waters Corporation: Waters Empower Custom Fields

Empower Custom Fields

In this session we demonstrate how to create and use Customer Fields to generate workflows in Empower

Waters Empower Custom Fields

Regardless of your sample, it is critical to simplify your routine analysis work and infuse as much automation as possible into your workflow so you can increase confidence in your results and reduce the time it takes to make decisions. Empower Software offers you the flexibility to optimize operations in your lab.
To ensure that your method is performing as expected, routine food analysis calls for calibration/quantitation along with system suitability and/or control samples. These applications call for some commonly made calculations such as:
<ul>
<li>Percent Difference compared to a Target Amount</li>
<li>Average Amount in Duplicate Samples</li>
<li>Percent Deviation for Standards</li>
</ul>
Determining authenticity of botanical ingredients used in dietary supplements can be challenging. This routine test is simplified by automating evaluation of the following:
<ul>
<li>Chromatographic Pattern</li>
<li>Mass Spectral Pattern</li>
<li>Spectral Library Match</li>
</ul>
To further streamline, using tailored reports in Empower Software makes interpretation of results easy and decision-making faster with simple pass/fail, so you know whether you can ship a batch.
Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.
<blockquote>
Presenter: Neil Lander (Principal Product Manager, Informatics, Waters Corporation)
</blockquote>
<blockquote>
Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.
</blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Streamlining Efficiencies in Your Routine Analysis – Food and Dietary Supplement Applications

LCMS webinars focusing on Software - page 28

Reducing Human Errors with Empower 3.8.0

Empower Tips: Ask Me Anything About Streamlining Efficiencies in Your Routine Analysis – Food and Dietary Supplement Applications

LC-MS for RNA Therapeutics to Ensure Product Quality and Process Consistency

Error-Free Sample Preparation Workflows

Empower Tips: Ask Me Anything About Data Flow and Data Process Mapping with Data Governance in Mind

Informatics series #13 Sample Weights in Empower

Clarity Tips & Tricks Webinar

The Digital Lab Connection

Empower Custom Fields

Adjust Empower very flexible to your specific needs

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