Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"><h4>The Role of Chromatography in Forensic Food Analysis</h4>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:<ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate adulteration.</li></ul>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.<h4>Isocratic vs. Gradient Elution</h4>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:<ul><li>Isocratic HPLC uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li>Gradient HPLC changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.<h4>Watrex Praha and the Contribution of Modern Systems to Food Analysis</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha, s.r.o.</a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline">Streamline™ product line</a> includes several HPLC system concepts suitable for food analysis:<ul><li>Isocratic HPLC system – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li>Binary gradient HPLC system – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures (e.g., phenolic profiles of wine, components of aromatic extracts).</li><li>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography) – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf">Product catalogue HERE</a></blockquote>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.<h4>Practical Examples of Adulteration Revealed by HPLC</h4><ul><li>Detection of olive oil dilution: HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li>Estimation of sugar addition in honey: Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li>Identification of aromatic adulterants in wine: Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4>Conclusion</h4>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.<blockquote>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true">LinkedIn</a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague">Facebook</a>) for more engaging topics.</blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.<h4>In this episode, you'll learn:</h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.<h4>Referenced Papers:</h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.<h3>Video Transcription</h3><h4>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</h4><h5>Introduction</h5>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.<h5>Research Focus: Malaria Drug Discovery and Kinase Targets</h5>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.<h5>Chromatography in Protein Expression and Purification Workflows</h5>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.<h5>Integrating Size Exclusion Chromatography into the Workflow</h5>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.<h5>The Role of Mass Spectrometry in Biomolecular Characterization</h5>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.These approaches are essential for understanding how candidate drug targets function within complex biological systems.<h5>Sample Preparation Challenges in Biological Research</h5>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.<h5>Advances in Chromatography and MS for Malaria Research</h5>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.<h5>Malaria, Drug Resistance, and Global Impact</h5>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.<h5>Advice for Early-Career Researchers</h5>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.<h5>Closing Remarks</h5>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

Article

Product

Video

Scientists

Food

Interview

Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

Agilent Technologies

Technology Networks

SelectScience

Wiley

SCIEX

The biopharma industry has used capillary electrophoresis for many years to help improve the quality of biopharmaceuticals. Traditionally, scientists have utilized single capillaries or chips in these systems, requiring samples to be run in series.
However, a new multi-capillary system was recently launched, enabling scientists to parallel process eight samples simultaneously providing a positive impact in the development of cell and gene therapies and therapeutic proteins.
In this webinar, you will:
<ul>
<li>Uncover how this system achieves CE-SDS separations of the same quality as traditional systems</li>
<li>Gain an understanding of how the system designers have prioritized ease of use</li>
<li>Discover how this system offers N-glycan analysis and charge variant detection</li>
<li>Explore how this novel approach is accelerating the development of cell and gene therapy and vaccine products</li>
</ul>
<blockquote>
Presenter: Dr. Stephen Lock (Senior Market Development Manager, Biopharma, SCIEX)
</blockquote>

Technology Networks: Empower Your Protein and Gene Therapy Development With Multi-Capillary Electrophoresis

Empower Your Protein and Gene Therapy Development With Multi-Capillary Electrophoresis

The biopharma industry has used capillary electrophoresis for many years to help improve the quality of biopharmaceuticals.

Protein and Gene Therapy Development With CE-SDS

In this talk, learn about the capabilities of the Agilent Oligo Pro II system and how it uses automated parallel capillary electrophoresis for purity analysis of oligonucleotides.
<blockquote>
Presenter: James Taub, PhD (Biopharma Market Specialist, Agilent Technologies, Inc.)
</blockquote>

Agilent Technologies: Chill out with the Agilent Oligo Pro II system

Chill out with the Agilent Oligo Pro II system

The Agilent Oligo Pro II system and how it uses automated parallel capillary electrophoresis for purity analysis of oligonucleotides.

There are hundreds of drug candidates to screen for purity, integrity and stability during early drug development, which can become a considerable bottleneck.
High-throughput analytical platforms and fast analysis methods are attractive approaches to address high sample volume issues. CE-SDS is a widely recognized workflow for the determination of purity in protein and gene therapy products. However, a major limitation of this method is its low throughput, resulting in highly inefficient method development and optimization when onboarding new molecules.
This webinar discusses how to assess the purity of mAb and viral vector samples using a capillary electrophoresis-based strategy that enables a significant reduction in turnaround time without compromising data quality. 
Attend this webinar to:
<ul>
<li>Learn about rapid method development for new modalities
</li>
<li>Discover reproducible characterization of impurities
</li>
<li>Enable an eight-fold increase in throughput
</li>
</ul>
<blockquote>
Presenter: Andy Blum (Scientist, Biogen)
</blockquote>

Technology Networks: Tackling Analytical Bottlenecks in Next-Generation Drug Development

Tackling Analytical Bottlenecks in Next-Generation Drug Development

How to assess the purity of mAb and viral vector samples using a capillary electrophoresis-based strategy.

Tackling Analytical Bottlenecks

This online event has been designed for our customers and brings together opinion leaders, top scientists and industry innovators to share their research in the biopharma field.
The technologies presented will be CE and LC-MS.
Agenda:
<h5>10:00–10:10 Welcome and introduction</h5>
<ul>
<li>Steve Lock, SCIEX</li>
</ul>
<h5>10:10–10:35 Use of LC-MS in process analytical testing as part of development of a continuous manufacturing workflow</h5>
<ul>
<li>Daniel Myatt / Ronan Gough, CPI</li>
</ul>
<h5>10:35–11:00 Tips and tricks for biotherapeutics characterization by peptide mapping</h5>
<ul>
<li>Giulia Calloni, SCIEX</li>
</ul>
<h5>11:00–11:25 Developing methods for SEC-MS and IEX-MS for the analysis of biotherapeutics</h5>
<ul>
<li>Sarah Nguyen, Strasbourg University</li>
</ul>
<h5>11:25–11:35 Break</h5>
<h5>11:35–12:00 The power of CZE analysis for characterization of NANOBODY® molecules</h5>
<ul>
<li>Dries Dejaegere, Sanofi</li>
</ul>
<h5>12:00–12:25 Multi-capillary gel electrophoresis to increase throughput for the purity analysis of biotherapeutic proteins</h5>
<ul>
<li>Theresa Merlino, The Janssen Pharmaceutical Companies of Johnson &amp; Johnson</li>
</ul>
<h5>12:25–12:50 A disruptive approach to the characterization of mAb charge variants during biopharmaceutical process development</h5>
<ul>
<li>Greg Adams, FUJIFILM</li>
</ul>
<h5>12:50–13:00 Wrap-up and Q&amp;A</h5>
<blockquote>
Presenter: Steve Lock, PhD (Snr. Marketing and Market Development Manager, Biopharma, EMEAI, SCIEX)
</blockquote>
<blockquote>
Steve obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry. He has worked for SCIEX in various technical roles for over 20 years and has presented at over 80 international meetings around the world. He currently works closely with customers in the biopharmaceutical industry. Steve is also currently an Adjunct Professor at the University of Pannonia.
</blockquote>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SCIEX_agenda_day_1_6818ee0331/SCIEX-agenda-day-1.jpg" alt="SCIEX - agenda day 1.jpg"/>

SCIEX: SCIEX Biopharma meeting - 3rd edition! Day 1 - Gene therapy, vaccines and oligonucleotides

SCIEX Biopharma meeting (Day 1) - Gene therapy, vaccines and oligonucleotides

Gene therapy, vaccines and oligonucleotides. The technologies presented will be CE and LC-MS.

Day 1 - Gene therapy, vaccines and oligonucleotides

This online event has been designed for our customers and brings together opinion leaders, top scientists and industry innovators to share their research in the biopharma field.
The technologies presented will be CE and LC-MS.
Agenda:
<h5>10:00–10:10 Welcome and introduction</h5>
<ul>
<li>Steve Lock, SCIEX</li>
</ul>
<h5>10:10–10:35 Particle seizing by CE</h5>
<ul>
<li>Hervé Cottet, Montpellier University</li>
</ul>
<h5>10:35–11:00 Application of CE for purity analysis in cell and gene therapy</h5>
<ul>
<li>Ryan Hylands / Luiza Chrojan, Pharmaron</li>
</ul>
<h5>11:00–11:25 Tips and tricks for success in CE analysis</h5>
<ul>
<li>Whitney Smith, SCIEX</li>
</ul>
<h5>11:25–11:35 Break</h5>
<h5>11:35–12:00 RNA payload analysis: development and analytical challenges</h5>
<ul>
<li>Jérémie Parot, SINTEF Industry</li>
</ul>
<h5>12:00–12:25 The use of CE for AAV analytics</h5>
<ul>
<li>Padraig Flaherty, MeiraGTx</li>
</ul>
<h5>12:25–12:50 Oligo analysis by LC-MS</h5>
<ul>
<li>Alexandra Bushby, Labcorp</li>
</ul>
<h5>12:50–13:00 Wrap-up and Q&amp;A</h5>
<blockquote>
Presenter: Steve Lock, PhD (Snr. Marketing and Market Development Manager, Biopharma, EMEAI, SCIEX)
</blockquote>
<blockquote>
Steve obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry. He has worked for SCIEX in various technical roles for over 20 years and has presented at over 80 international meetings around the world. He currently works closely with customers in the biopharmaceutical industry. Steve is also currently an Adjunct Professor at the University of Pannonia.
</blockquote>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SCIEX_agenda_day_2_b442145213/SCIEX-agenda-day-2.jpg" alt="SCIEX - agenda day 2.jpg"/>

SCIEX: SCIEX Biopharma meeting - 3rd edition! Day 2 - Therapeutic proteins and peptides

SCIEX Biopharma meeting (Day 2) - Therapeutic proteins and peptides

Therapeutic proteins and peptides. The technologies presented will be CE and LC-MS.

Day 2 - Therapeutic proteins and peptides

Parallel capillary electrophoresis is a powerful technique for automated protein analysis. The ProteoAnalyzer system is a novel instrument that uses this technique to overcome the limitations of traditional methods. It features 12 capillaries, validated reagents, and automated cleaning and rejuvenation after each run, enabling the user to obtain high-quality data for various protein sample types.
In this webinar, Dr. Kyle Luttgeharm, Product Manager and Dr. Gizem Bergdahl, Research Scientist, DAKO Denmark R&amp;D &amp; Manufacturing, will discuss how the ProteoAnalyzer system can transform protein analysis workflows.
Key learning objectives
<ul>
<li>Understand more about the ProteoAnalyzer</li>
<li>Learn how to improve the consistency of your protein analysis results</li>
<li>Explore how the ProteoAnalyzer can be used to analyze challenging sample types including total protein extracts and crude cell lysates</li>
</ul>
Who should attend?
<ul>
<li>Anyone who needs help to gain laboratory efficiency, free up technician time, and minimize delays due to ambiguous results leading to re-sample analysis.</li>
</ul>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Dr. Kyle Luttgeharm (Product Manager, Agilent Technologies)
</blockquote>
<blockquote>
Dr. Kyle Luttgeharm is a Product Manager with Agilent Technologies. He holds undergraduate degrees in Biology and Biochemistry from Southwestern College in Winfield, Kansas, and a doctorate in Biochemistry from the University of Nebraska-Lincoln. Dr. Luttgeharm previously worked as an Application Scientist with Advanced Analytical Technologies, Inc. and as an R&amp;D Scientist with Agilent Technologies. He has extensive experience developing methodologies and assays for Agilent’s parallel capillary electrophoresis technology.
</blockquote>
<blockquote>
Presenter: Dr. Gizem Bergdahl (Research Scientist, DAKO Denmark R&amp;D &amp; Manufacturing, Agilent Technologies, (Glostrup, Denmark)
</blockquote>
<blockquote>
Dr. Gizem Bergdahl has a deep understanding of diagnostics, clinical biochemistry, human diseases, and in vitro diagnostics, and extensive experience in areas including immunoassays (ELISA, immunoturbidimetry, and chemiluminescence), ligand-binding assays (SPR), antibody conjugation to latex, and magnetic beads.
</blockquote>
<blockquote>
Presenter: Blake Forman (Scientific Content Creator, SelectScience)
</blockquote>
<blockquote>
Blake studied chemistry at the University of Surrey, UK, and further went on to study a master’s in chemistry at the University of Southampton, UK. Blake has previously worked in the Medicines and Healthcare Products Regulatory Agency (MHRA) lab at LGC and as a technical sales specialist for Waters. As a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience.
</blockquote>

Agilent: Improve laboratory efficiency with automated protein analysis

Improve laboratory efficiency with automated protein analysis

In this webinar, will be discussed how the ProteoAnalyzer system can transform protein analysis workflows.

Improve lab efficiency with automated protein analysis

Reliable and robust analysis is essential throughout in vitro transcription (IVT) mRNA workflows, providing important quality information to help minimize risk and aid in the production of a consistent product. In this webinar, our expert speakers will discuss how the Agilent automated electrophoresis systems are ideal for this assessment, providing fragment sizing and mRNA integrity at a variety of checkpoints throughout the process.
<h5>Key learning objectives</h5>
<ul>
<li>Learn about the quality control (QC) steps that can be performed by the Fragment Analyzer and how they can improve the IVT process, including:<ul>
<li>Quality and size of the linearized plasmid size</li>
<li>Purity analysis of the IVT mRNA</li>
</ul>
</li>
</ul>
Who should attend?
Researchers, lab managers, lab directors, QA/QC managers doing IVT mRNA analysis in biotherapeutics research and production.
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Whitney Pike (Application Scientist, Agilent Technologies)
</blockquote>
<blockquote>
Whitney Pike joined Agilent in 2016 and is an application scientist with a focus on Agilent’s automated electrophoresis instruments. She played a pivotal role in the release of the Femto Pulse system as a scientist on the product development team. She completed her master&#39;s degree in Biology, with an emphasis on cellular, molecular, and developmental biology, before working at a core facility at Iowa State University, responsible for performing next-generation sequencing studies.
</blockquote>
<blockquote>
Presenter: Dr. Leslie Friedmann (Product Manager Parallel CE systems, Agilent Technologies)
</blockquote>
<blockquote>
Dr. Leslie Friedmann joined Agilent in 2017 as a Product Manager for the Automated Electrophoresis portfolio. She focused first on the ScreenTape portfolio and is now responsible for the parallel CE instruments. Her education includes a diploma in biology from the University of Freiburg, Germany, a PhD in biology from the University of Frankfurt, Germany and a post-doctoral fellowship at the University of Singapore in developmental biology using molecular biology and gene expression analysis techniques.
</blockquote>
<blockquote>
Presenter: Professor Harris Makatsoris (Professor Of Manufacturing Systems in the Department of Engineering, King’s College London)
</blockquote>
<blockquote>
Harris is Professor Of Manufacturing Systems in the Department of Engineering at King’s College London and the founder of Centillion Technology Limited, a UK based biotech specialising in the rapid scaling and manufacture of RNA-based therapeutics and vaccines. He has a process engineering background with 27-years experience in both industry and academia. In all his past and present roles, he is managing interdisciplinary projects that require integration and coordination. Harris is an expert in continuous RNA manufacturing. His work is focusing on addressing the challenges in the rapid design, development and scalable manufacture of RNA and other nucleic acids and has pioneered the application of flow technologies in RNA manufacture, of various types. His research has attracted over £26m and he is currently leading a unique multi-disciplinary, multi-million pound programme comprising both industry and academia, building the Biofoundry-in-a-Box (BiaB). The BiaB is a unique portable, multi purpose RNA microfactory designed for the rapid design and deployment of a range of therapeutics and vaccines, with Low and Middle Income Countries in mind. He has several patents in his name in the space of RNA manufacture and continuous bioprocessing. He is highly experienced in technology transfer to industry through a variety of commercial routes.
</blockquote>
<blockquote>
Presenter: Jemima Arnold (Editorial Assistant, SelectScience)
</blockquote>
<blockquote>
Jemima studied Cellular and Molecular Medicine at the University of Bristol, UK, and as a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
</blockquote>

SelectScience: Best Practices for Analysis of IVT mRNA Integrity using the Agilent Fragment Analyzer Systems

Best Practices for Analysis of IVT mRNA Integrity using the Agilent Fragment Analyzer Systems

Speakers will discuss how the Agilent automated electrophoresis systems are ideal for assessment of in vitro transcription (IVT) mRNA workflows.

Analysis of IVT mRNA using the Agilent Fragment Analyzer

Join us as we delve into the essential principles of Capillary Electrophoresis (CE) and explore its diverse applications across various scientific disciplines. Our industry expert Nandakumar Sundaramoorthy, Ph.D. will share valuable insights and practical knowledge to enhance your understanding of CE.
<h5>Discussion points:</h5>
<ul>
<li>CE and CE-MS: general theory, instrumentation and it&#39;s classification, applications and therapeutic areas</li>
<li>Significance of CE in the world of biotherapeutics and other bio-related products</li>
<li>Breakthrough of CE-based separation in novel biologics</li>
<li>Case study: CE-SDS and cIEF/CZE related problems, root cause and solutions</li>
<li>Quality control, regulatory expectations, and pharmacopeial evolution for CE-based methods</li>
</ul>
<blockquote>
Presenter: Dr. Nandakumar Sundaramoorthy
</blockquote>
<blockquote>
He is a scientific professional with nearly two decades of global experience, holding a Ph.D. in Applied Proteomics. He is currently the Director - Analytical Development and Characterization at Kemwell Biopharma Pvt Ltd. He has a proven track record of successfully leading end-to-end projects, specializing in protein characterization.
</blockquote>

SCIEX: Fundamentals of capillary electrophoresis (CE)

Fundamentals of capillary electrophoresis (CE)

Seznamte se s námi se základními principy kapilární elektroforézy (CE) a prozkoumejte její rozmanité aplikace v různých vědních oborech.

We want to Help you gain the advantages from advanced automated electrophoresis solutions. Solutions that you’ll need to accelerate your next-generation sequencing projects, cell-free DNA and genomic DNA analysis, RNA and proteins quality controls.
Does your lab need a Tech Upgrade? A technology upgrade may help you save time and money. During the workshop, you’ll hear the benefits your lab can get if you upgrade.
Come to the Workshop to:
<ul>
<li>Learn how the Advanced AE platforms perform in terms of hardware, software, and assays as well as workflows.</li>
<li>See equivalent data between the Bioanalyzer, TapeStation, and Fragment Analyzer system.</li>
<li>Discover the key advantages and benefits of the advanced platforms.</li>
<li>Listen to a customer experience.</li>
</ul>
<blockquote>
Presenter: Dr. Matthias Meister (Product Manager, Agilent Technologies Germany)
</blockquote>
<blockquote>
Matthias Meister is Product Manager at Agilent for the Bioanalyzer portfolio. He joined the marketing team for Automated Electrophoresis in 2022 and is based in Waldbronn, Germany. Prior to joining Agilent, he worked as a postdoctoral researcher at the German Cancer Research Center (DKFZ) in Heidelberg, where he also received his PhD.
</blockquote>
<blockquote>
Presenter: Sébastien Boulanger (Scientific Expert, Institute of Pathology and Genetics (IPG), Belgium)
</blockquote>
<blockquote>
Sébastien Boulanger integrated the Institute of Pathology and Genetics (IPG) of Gosselies as a lab technician in the molecular biology department in 1999. IPG is one of the eight Genetics Centers recognized in Belgium.
</blockquote>
<blockquote>
He gradually moved to a more scientific position and currently recognized by the Belgian society of Human Genetics as a Medical Genetics Laboratory Expert being now one of the main responsible of the technical lab development.
</blockquote>

Agilent: Upgrade your Tech with Agilent Advanced Automated Electrophoresis Solutions

Upgrade your Tech with Agilent Advanced Automated Electrophoresis Solutions

We want to help you gain the advantages from advanced automated electrophoresis solutions.

Agilent Advanced Automated Electrophoresis Solutions

For years, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) has been the go-to method for protein purity analysis. Despite shortcomings in sensitivity, reproducibility, and its semi-quantitative nature, SDS-PAGE is still widely used.
Capillary electrophoresis (CE-SDS) with its superior separation power, accurate quantitation, and ability for automation is quickly replacing SDS-PAGE in laboratories around the world. In addition to the obvious advantages in time and labor, CE-SDS also stands out as a ‘greener’ method over SDS-PAGE. As laboratories worldwide increasingly focus on waste management with various methods, CE-SDS presents clear advantages over SDS-PAGE for protein purity analysis.​
In this webinar, Prof. Hermann Wätzig from the Institute of Medicinal and Pharmaceutical Chemistry, Braunschweig, Germany and Dr. Chris Heger, Director, Applications Science, Bio-Techne will discuss how transitioning from SDS-PAGE to CE-SDS benefits research, both from an academic and industrial perspective. The speakers will also discuss the current state-of-the art instrumentation and method development landscape.
<blockquote>
Presenter: Dr. Hermann Wätzig (Professor, Technische Universität Braunschweig)
</blockquote>
<blockquote>
Presenter: Dr. Chris Heger (Director, Applications Science, Bio-Techne)
</blockquote>

Wiley: Time to Turn the PAGE on SDS-PAGE!

Time to Turn the PAGE on SDS-PAGE!

Transitioning from SDS-PAGE to CE-SDS benefits research, both from an academic and industrial perspective.

LCMS webinars focusing on Electrophoresis - page 3