Ask any analytical chemist which hyphenated technique has become indispensable over the past decade, and the answer will almost certainly be the same: tandem chromatography–mass spectrometry. Whether it is <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis">LC–MS/MS</a> quantifying a novel biologic’s metabolic fate, or <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis">GC–MS/MS</a> chasing per- and polyfluoroalkyl substances (PFAS) down to sub-part-per-trillion levels in municipal water, the combination of chromatographic separation with the specificity of tandem mass detection has become the analytical workhorse of choice across virtually every regulated sector.Below we examine the key forces propelling this expansion, from biopharma pipeline dynamics and PFAS regulatory mandates to AI-driven automation and reshoring of pharmaceutical manufacturing—and explore what each trend means for laboratories managing sample preparation and analytical workflows.<h3>Biopharma: The Engine Room of LC–MS/MS Demand</h3><h4>GLP-1 Agonists and the Metabolomics Gold Rush</h4>The explosive commercial and scientific interest in GLP-1 receptor agonists—<a target="_blank" rel="noopener noreferrer" href="https://www.sepscience.com/advancing-glp-1-peptide-analysis-chromatography-challenges-and-innovations-12163">semaglutide and related peptide therapeutics </a>for diabetes and obesity—has dramatically expanded the analytical demands placed on tandem MS platforms. Discovery programs require precise quantification of peptide drug candidates and endogenous biomarkers across complex biological matrices. These workflows rely on high-sensitivity LC–MS/MS for pharmacokinetic profiling, receptor occupancy studies, and metabolomics characterization. Metabolomics is, in fact, projected to be the fastest-growing application segment in mass spectrometry, driven in part by its growing role in cancer screening, biomarker discovery, and metabolic disease research.I have been spending more time learning about GLP-1s because this is the hot topic that people want to discuss on the Concentrating on <a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL?si=n9GLz9zjSCOrVfXPxFPhng&amp;nd=1&amp;dlsi=52868e027aad4d22">Chromatography podcast</a>. With that said, I was astounded looking at CNBC.com during breakfast this morning to see that <a target="_blank" rel="noopener noreferrer" href="https://www.cnbc.com/2026/03/21/glp-1-diets-restaurants-protein-fiber-weight-loss-drugs.html">one in eight Americans are currently taking one of these drugs such as Ozempic</a>.<h4>Antibody–Drug Conjugates and Gene Therapy: Complexity at Every Step</h4>The ascent of antibody–drug conjugates (ADCs) and gene therapies has introduced a new level of analytical complexity. ADCs blend the target specificity of monoclonal antibodies with the cytotoxic potency of small-molecule payloads—and characterizing the linker chemistry, drug-to-antibody ratio (DAR), and conjugate stability demands high-resolution LC–MS/MS at every stage from discovery through QC release testing. Gene therapy vectors require equally rigorous characterization of impurities, aggregates, and empty/full capsid ratios.This translates into a direct need for low-evaporation, high-recovery concentration methods that preserve labile protein structures and maintain sample integrity prior to LC–MS/MS injection. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">Nitrogen evaporation workflows</a> that minimize oxidative stress and maintain sample temperature are increasingly critical in these pipelines.<h4>Precision Medicine and Multi-Omics Integration</h4>The shift toward precision medicine has accelerated adoption of high-throughput, multi-analyte MS platforms. Proteomics, metabolomics, and lipidomics workflows are converging on a single analytical platform—tandem MS—and generating datasets of a scale and complexity that were unimaginable ten years ago.Make no mistake: the triple quadrupole serves an irreplaceable role in quantitative targeted analysis. At the same time, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=134&amp;manufacturerItems=3">Orbitrap </a>and Q–TOF hybrid platforms are growing rapidly as researchers demand full-scan accurate mass for untargeted discovery.<h3>Environmental Mandates: PFAS Testing Drives Adoption</h3>Environmental testing is something that every person, chemist or not, can understand the importance of. Mounting mandates from the U.S. Environmental Protection Agency and European regulators regarding PFAS—the “forever chemicals” whose extraordinary environmental persistence is matched only by the analytical challenge of detecting them at trace levels—have created immediate, compliance-driven investment in LC–MS/MS infrastructure.<h4>The Regulatory Framework Chemists Need to Know</h4>The EPA has codified a suite of LC–MS/MS-based methods as the analytical standard for PFAS in water and solid matrices:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/epa-533-drying-down-pfas-samples-after-spe">EPA Method 533</a>: Weak anion exchange SPE combined with LC–MS/MS; covers 25 PFAS including short-chain compounds in drinking water.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/sample-concentration-epa-method-537-pfas-determination">EPA Method 537.1</a>: Reversed-phase SPE followed by LC–MS/MS; covers 18 PFAS including the GenX compound, with method detection limits in the low ng/L range.</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/en/solutions/environmental/water-testing/pfas-in-water/us-epa-method-8327?srsltid=AfmBOooCY1pcavj7B1PH-75d3HO4BGDglNlhmyTyrpDY3zvUksMO1dlg">EPA Method 8327</a>: LC–MS/MS for selected PFAS in prepared extracts of aqueous and solid matrices.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/preparing-diverse-sample-matrices-for-epa-method-1633">EPA Draft Method 1633</a>: The emerging benchmark for PFAS in non-aqueous matrices—aqueous, solid, biosolids, and tissue—quantifying up to 40 PFAS.</li></ul>All of these methods designate LC–MS/MS, specifically LC coupled to triple quadrupole or tandem-in-time platforms operating in negative electrospray ionization mode with multiple reaction monitoring (MRM), as the required or recommended analytical technique. PFAS analysis is, at its core, an LC–MS/MS problem.<h4>The Sample Preparation Bottleneck</h4>Chemists who have worked with PFAS methods understand the particular demands they place on sample preparation. The ubiquity of<a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/tackling-pfas-contamination-practical-insights-from-epa-537.1-method-development"> PFAS in laboratory materials</a>—PTFE tubing, some SPE sorbents, standard bench supplies—means that blank contamination control is as analytically demanding as the detection itself. EPA 537.1 requires that sample volumes of approximately 250 mL be extracted and reconstituted in as little as 1 mL, creating a 250:1 concentration factor that can expose even trace contamination in the sample processing chain. The installation of a delay column between the LC pump and autosampler to prevent instrument background interference is now standard practice.This is precisely where robust, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5385">inert nitrogen evaporation </a>systems earn their keep. PFAS workflows that use nitrogen-assisted evaporation to concentrate extracts prior to reconstitution benefit from materials that avoid PTFE and other fluoropolymers that can leach PFAS background—a consideration Organomation addresses in its concentration system designs.The analytical community is also moving beyond targeted PFAS analysis. High-resolution accurate mass (HRAM) spectrometry via Orbitrap technology, paired with non-targeted screening workflows, is gaining traction for the identification of the more than 5,000 currently catalogued PFAS compounds and the novel structures that continue to emerge. LC–MS/MS remains the quantitative gold standard; HRAM provides the discovery layer.<h3>Technological Trends Reshaping the LC–MS/MS Workflow</h3><h4>Artificial Intelligence and Automated Data Interpretation</h4>The datasets generated by modern tandem MS platforms—particularly in omics applications—are too large and too complex for conventional manual review. AI-driven spectral deconvolution, automated peak integration, and AI-assisted hypothesis generation are now embedded in the software suites accompanying leading instruments. <a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/about/newsroom/presrel/2022/17feb-gp22004.html?srsltid=AfmBOop3warRIaEojXM2YyMML6RXfMIDzE-Wm0h-SiKkkocBeeET2m7u">Agilent’s acquisition of Virtual Control’s ACIES </a>software specifically to automate LC–MS and GC–MS data analysis is emblematic of this direction.For laboratory managers, AI integration means smaller specialized teams can operate high-throughput multi-analyte workflows—but it also means that the front-end sample preparation must be even more reproducible, since algorithmic interpretation amplifies inconsistencies introduced upstream.<h4>Online SPE Coupling and “Chromatography Without Walls”</h4>A significant trend is the integration of solid-phase extraction directly into the HPLC flow path—so-called online SPE–HPLC coupling. Systems that combine extraction and chromatographic separation in a single analytical instrument reduce manual transfer steps, minimize the risk of sample loss or contamination, and dramatically increase throughput. For analytes at very low concentrations, online SPE provides the on-column concentration capability that traditional offline preparation achieves with manual evaporation steps.That said, offline sample preparation—including <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">nitrogen-assisted evaporation</a> under controlled conditions, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2407">liquid–liquid extraction</a>, and protein precipitation—retains significant advantages in flexibility, matrix handling, and the ability to process sample volumes that exceed online system capacity. The two approaches are increasingly complementary rather than competitive in well-designed analytical laboratories.<h4>High-Purity Solvents and Reference Materials</h4>As detection limits push further into the femtogram range and regulatory methods demand demonstrable blank performance, the quality of analytical consumables—mobile phase solvents, derivatization reagents, reference standards—has moved from background consideration to front-of-mind. Method validation in regulated environments now routinely requires vendor certification of solvent purity at a level that directly interfaces with instrument performance.<h3>The Ever-Present Need for Sample Evaporation</h3>As LC–MS/MS methods push detection limits lower, the analytical burden shifts increasingly upstream. The quality of the concentration, cleanup, and reconstitution steps preceding injection determines whether the instrument’s theoretical sensitivity is achievable in practice.Several specific developments are particularly relevant:<ul><li>ADC and gene therapy characterization workflows require gentle, low-temperature evaporation that preserves protein structure and prevents aggregation prior to LC–MS/MS. Nitrogen evaporation under controlled conditions is the preferred approach.</li><li>PFAS trace analysis demands evaporation systems constructed from PFAS-inert materials, with rigorous attention to avoiding fluoropolymer surfaces throughout the sample path.</li><li>High-throughput pharmaceutical QC driven by reshoring demands parallel processing capability—multi-position evaporators that maintain consistent flow and temperature across all positions simultaneously.</li><li>Multi-omics and metabolomics workflows generate large sample batches that benefit from automated, walk-away evaporation with programmable endpoints to ensure reproducible final volumes across a cohort.</li></ul>Tandem chromatography–mass spectrometry is not merely growing; it is becoming structurally embedded in the analytical workflows of every major regulated industry. The convergence of biopharma complexity, PFAS regulatory mandates, AI-enabled data analysis, and pharmaceutical reshoring is creating a multi-year demand cycle that will require laboratories to invest not only in instruments, but in the entire sample preparation ecosystem that makes those instruments perform.For analytical chemists, the practical takeaway is this: as mass spectrometry sensitivity continues to advance, the sample preparation step becomes the binding constraint on method performance. Whether you are developing a new PFAS method to meet EPA 1633, characterizing an ADC’s drug-to-antibody ratio, or running a 500-sample metabolomics cohort overnight, the reliability and cleanliness of your concentration and cleanup workflow determines the quality of every data point that follows.&nbsp;

The Continued Rise of Tandem Chromatography–Mass Spectrometry

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

Article

Product

Application

Academy

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

New Food magazine

Agilent Technologies

Phenomenex

Waters Corporation

You ran your HPLC and sample yesterday. Everything looked as expected. Today, the chromatography looks different, so it must be the column, correct? Not so fast. We can be quick to assume that the problem with our chromatography is with the HPLC column, but often there is another reason why the chromatography has changed.
In this seminar, we will discuss practical steps to identify the source of the problem and troubleshoot common issues. Considerations from sample preparation, mobile phase preparation, the column, to the HPLC system itself will be discussed.
Having an understanding for where problems can occur helps us quickly identify the problem, correct it, and prevent it from happening again.
<blockquote>
Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
</blockquote>

It Isn’t Always the Column: Troubleshooting Your HPLC Separation

We will discuss practical steps to identify the source of the problem and troubleshoot common issues.

It Isn’t the Column: Troubleshooting Your HPLC Separation

Having good peak shape can improve resolution and produce accurate and reliable quantitation results. Problems with peak shape, like fronting, tailing, shoulders, and splits, are all indicative of chromatography issues.
We will discuss factors affecting peak shape, such as the column, sample, mobile phase, and more, and offer remedies for improving peak shape.
<blockquote>
Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.
</blockquote>
<blockquote>
Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.
</blockquote>
<blockquote>
Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
</blockquote>

Peak Shape and Why It Matters: How to Get Good Peak Shape

We will discuss factors affecting peak shape, such as the column, sample, mobile phase, and more, and offer remedies for improving peak shape.

Purification is critical to many scientific workflows. Compounds of interest are rarely found to be the right purity in their native state. Join Waters and Virscidian for this 6-part webinar series to learn the basics of purification and application of purification principles for more advanced workflows.
<h3>PART 5 | Request to Results: Automating Purification Decisions</h3>
High quality purification software demands superior data processing, efficient handling of information and metadata, and trustworthy results. Is all this really possible in such a complex, multi-stage workflow? Tune in to learn about Virscidian’s capabilities and ways to bring more value to your lab. 
Learning Objectives: 
<ul>
<li>Ways to reduce the cycle time from crude reaction to purified product</li>
<li>How Virscidian is tackling complex challenges in purification</li>
<li>How decisions made manually in your laboratory today can be automated</li>
</ul>
<blockquote>
Presenter: Mark Spears, Ph.D. (Senior Scientist, Virscidian)
</blockquote>
<blockquote>
Mark Spears is a Senior Scientist at Virscidian and is passionate about making each customer’s research a success story. In addition to supporting purification, he leads the HTE and affinity selection mass spec teams at the company. Mark has developed expertise across research disciplines and brings a breadth of experience from the laboratory and industry vendors.
</blockquote>

Waters Corporation: Request to Results: Automating Purification Decisions

Request to Results: Automating Purification Decisions

Learn about Virscidian’s capabilities and ways to bring more value to your lab.

Purification is critical to many scientific workflows. Compounds of interest are rarely found to be the right purity in their native state. Join Waters and Virscidian for this 6-part webinar series to learn the basics of purification and application of purification principles for more advanced workflows.
<h3>PART 6 | Cost-Effective Oligonucleotide Purification and Scaling without HFIP</h3>
Chemists face hurdles to achieving cost-effective laboratory scale purification of oligonucleotides; coping with expensive HFIP ion pairing mobile phases, and scaling from analytical UPLC methods to semi-preparative scale separations is not as simple as it seems. Here we present the impact of several techniques in the purification of a 20-mer oligo in the absence of HFIP. 
Learning Objectives: 
<ul>
<li>Recognize alternative separations chemistry for purification</li>
<li>Balance purity and recovery requirements</li>
<li>Learn purification techniques to employ in the laboratory</li>
</ul>
<blockquote>
Presenter: Andrew Leightner (Market Development Manager – Americas Field Marketing, Waters Corporation)
</blockquote>
<blockquote>
Andy Leightner has over 10 years of experience serving the pharma market from contract research organizations. He specialized in method development and validation leveraging LC, GC, and mass spectrometry to meet complex client needs. He supports pharmaceutical and biopharmaceutical discovery and development in the Americas for Waters Corporation. Andy received a bachelors degree in biology from the University of Wisconsin-Madison.
</blockquote>

Waters Corporation: Cost-Effective Oligonucleotide Purification and Scaling without HFIP

Cost-Effective Oligonucleotide Purification and Scaling without HFIP

Here we present the impact of several techniques in the purification of a 20-mer oligo in the absence of HFIP.

Cost-Effective Oligonucleotide Purification and Scaling

Are strong solvent effects after QuEChERS a challenge in your LC or LC/MS analysis? Agilent Feed Injection offers a solution. Learn more about this technology and application examples from food safety in this webinar.
In recent years, QuEChERS has greatly simplified sample preparation in food safety analysis. However, downstream of QuEChERS, a strong solvent sample can cause strong solvent effects in LC and LC/MS analysis, leading to peak breakthrough and poor peak shapes, especially as the injection volume increases.
Agilent Feed Injection offers a solution to this challenge. The technique mediates the strong solvent effects by infusing the sample into the mobile phase stream. This allows you to achieve perfect peak shapes and easy peak integration without the need for additional sample preparation.
Join this webinar for a comprehensive introduction to Agilent Feed Injection from Dr. Juan Liang-Schenkelberg, Product Manager, and Dr. Edgar Naegele, Application Scientist, from Agilent. The presentation will be complemented by application examples from the field of food safety analysis.
Learning points:
<ul>
<li>Learn about common challenges in LC and LC/MS analysis following QuEChERS.
</li>
<li>Learn about Agilent Feed Injection technology and how it helps overcome these challenges.
</li>
<li>Discuss application examples from the field of food safety analysis with our expert speakers.
</li>
</ul>
<blockquote>
Presenter: Dr. Juan Liang-Schenkelberg (Strategic Product Manager at Agilent Technologies)
</blockquote>
<blockquote>
Juan Liang-Schenkelberg holds a PhD in biochemical engineering from the Hamburg University of Technology (TUHH) in Germany. Juan joined Agilent Technologies in 2016 as Product Manager of analytical HPLC. She is currently responsible for high-end UHPLC systems and for the food segment.
</blockquote>
<blockquote>
Presenter: Dr. Edgar Naegele (Application Scientist at Agilent Technologies)
</blockquote>
<blockquote>
Edgar Naegele holds a PhD in organic chemistry from the Karlsruhe Institute of Technology (KIT) in Germany. Edgar joined Agilent Technologies in 2001 as an Application Scientist for LC and MS. Edgar is currently focused on the application of online LC and the new Agilent 1260 Infinity II Hybrid Multisampler in the pharmaceutical and food industries.
</blockquote>

Less work, better peaks

Are strong solvent effects after QuEChERS a challenge in your LC or LC/MS analysis? Agilent Feed Injection offers a solution.

MOSH and MOAH refer to Mineral Oil Saturated Hydrocarbons and Mineral Oil Aromatic Hydrocarbons. These mineral oils are hydrocarbon mixtures containing hundreds of chemical compounds of various sizes and structural configurations which are mainly derived from crude oil. Also, they are derived from liquefaction of coal, natural gas, and biomass.
MOAH are hydrocarbons majorly consisting of highly alkylated mono- and/or poly-aromatic rings. These compounds can be found everywhere in the environment, whereas MOSH are paraffin-like, open chained, commonly branched hydrocarbons (e.g., alkanes) and naphthene-like cyclic hydrocarbons (cycloalkanes).
The analysis of such contaminants in food is a challenging task and requires a comprehensive approach to tackle the cumbersome issues related to their determination. Additionally, their toxicity is still under investigation and requires further studies supported by more detailed analytical data.
In this live webinar in association with Gulf Bio Analytical LLC (GBA), you will learn:
1. Basics of MOSH/MOAH Analysis
2. Tackling challenges of MOSH/MOAH Analysis
3. Hyphenated chromatographic techniques as powerful tools to gain deeper insights into the MOSH and MOAH problem
Who should attend: Lab chemists, scientists, R&amp;D chemists, lab managers, technical assistants, food chemists
Why you should attend: To learn and mitigate challenges of MOSH/MOAH analysis through unique technologies
Hurry, register today.
<blockquote>
Presenter: Sajit Parameshwaran (Product Manager, Chromatography and Mass Spectrometry, Gulf Bio Analytical LLC (GBA))
</blockquote>
<blockquote>
Sajit Parameshwaran is currently working as a Product manager, Chromatography &amp; Mass Spectrometry in the Gulf Bioanalytical group, located in Dubai. He has completed his Masters in Analytical chemistry from Mumbai university. Prior to joining Gulf Bioanalytical, he worked as an application specialist &amp; HPLC trainer at Agilent Technologies India Pvt. Ltd. He has more than 20 years of experience in the analytical instrumentation industry. In the current organization, he has been instrumental in delivering application training and method setup on chromatography and mass spectrometry instruments related to food testing, forensics, petrochemicals and pharmaceuticals. In his current role, he is responsible to provide the right instrument solutions based on customer’s application requirements. He is actively involved in delivering talks in various webinars &amp; seminars on new product technologies and applications catering to applied markets.
</blockquote>

Agilent Technologies: Let's Talk MOSH/MOAH Analysis

Let's Talk MOSH/MOAH Analysis

Basics of MOSH/MOAH Analysis. Tackling challenges of MOSH/MOAH Analysis. Hyphenated chromatographic techniques.

This webinar will provide a detailed understanding of new revisions to allowed adjustments for USP and Ph. Eur. HPLC methods through the use of case studies.
<ul>
<li>Intro: Review revisions to allowed adjustments for USP and Ph. Eur.</li>
<li>Isocratic Case Study: Adjustments to column length and ID, particle size, and flow rate</li>
<li>Gradient Case Study: Adjustments to column length and ID, particle size, flow rate, and gradient timetable</li>
</ul>
Key Learning Points:
<ul>
<li>Review of recent revision to allowed adjustments for USP and Ph. Eur. HPLC methods</li>
<li>Utilize a case study to highlight the approach for making adjustments to an isocratic method and the resulting benefits</li>
<li>Understand the benefits of working within the allowed adjustments for improving the performance of compendial HPLC methods (isocratic and gradient) in your laboratory</li>
</ul>
Who Should Attend:
Scientists and analysts in the pharmaceutical industry who work with Pharmacopeia HPLC methods that are interested in:
<ul>
<li>Improving performance</li>
<li>Reducing analysis time</li>
<li>Understanding allowable adjustment application examples</li>
</ul>
<blockquote>
Presenter: Phil Koerner (Global Pharmaceutical Market Development Manager, Phenomenex)
</blockquote>

Phenomenex: Allowed Adjustments to Pharmacopeia HPLC Methods

Allowed Adjustments to Pharmacopeia HPLC Methods

This webinar will provide a detailed understanding of new revisions to allowed adjustments for USP and Ph. Eur. HPLC methods through the use of case studies.

<h5>Solving Problems that Matter: Risk Mitigation in the Pharma QC Laboratory</h5>
Expert Insights 3-Part Webinar Series: Risk Assessment, Control Strategies, and Intuitive Innovation to Improve Data Quality, Data Integrity and Lab Productivity. 
Pharma QC labs are facing increasing pressure on the cost of quality while maintaining compliance and assuring patient safety. From the harmonization of USP &lt;621&gt; to Empower™ workflows supporting data integrity to improving method transfer outcomes, you will learn from subject matter experts how to reduce errors and mitigate risk for reproducible data and enhanced productivity. 
Who should attend
<ul>
<li>Pharma QC Lab directors, managers and senior scientists with a drive to reduce errors, improve lab productivity and enhance data quality &amp; integrity.</li>
</ul>
<h3>PART 1 l The Harmonization of the USP General Chapter &lt;621&gt; Chromatography</h3>
<ul>
<li>Wednesday, August 2nd, 11:30-12:30 ET</li>
</ul>
This webinar will cover the harmonization process of the USP general chapter &lt;621&gt; Chromatography (harmonized with Ph. Eur. And JP) which became official in the USP-NF on December 1, 2022.
In this seminar, major changes and their implications will be discussed including changes in terminology and definition of chromatographic parameters, new system suitability requirements and allowed adjustments of chromatographic conditions for gradient elution.
<blockquote>
Presenter: Amanda Guiraldelli, Ph.D. (Scientific Affairs Manager, U.S. Pharmacopoeia)
</blockquote>
<blockquote>
Amanda Guiraldelli has been with USP since 2012 and holds the position of scientific affairs manager and principle scientist in the compendial science group-general chapters. She also serves as scientific liaison for the USP Measurement and Data Quality Expert Committee, where she works to develop and revise USP standards related to analytical procedure lifecycle/validation. Previously, Amanda worked as senior scientist at the USP reference standard laboratory for 8 years with characterization of compendial standards. She is visiting professor at the Institute of Chemistry at the University of Campinas (UNICAMP) in Brazil and is a frequent speaker and instructor on topics related to analytical procedure life cycle, Analytical Quality by Design (AQbD) and Chromatography. Amanda is specialist in mass spectrometry, chromatography, omics and chemometrics and has more than 15 years of experience in pharmaceutical R&amp;D areas. Prior to joining USP, she was R&amp;D scientist in a brazilian pharmaceutical industry and visiting scientist at TU Berlin in Germany and Leiden University in Netherlands (Center for Proteomics and Metabolomics) working on proteins characterization by LC-HRMS and procedure development using UHPLC-HRMS. Amanda is graduated in pharmacy biochemistry and holds a Ph.D. in analytical chemistry from the FCFRP - University of São Paulo (metabolomics by UHPLC-HRMS, GC-MS and 1H NMR and chemometrics).
</blockquote>

The Harmonization of the USP General Chapter 621 Chromatography

The harmonization process of the USP general chapter <621> Chromatography which became official in the USP-NF on December 1, 2022.

The USP General Chapter 621 Chromatography

Analytical methods are major quality control and integrity tools for biotherapeutic drug development. Transferring these methods to one or more receiving sites or scaling them during the drug development process or post-approval is a typical, yet critical activity to meet drug development timelines. Therefore, method transfer and scaling activities must be managed effectively.
Key Learning Points:
<ul>
<li>Learn key factors of method scaling and transfer</li>
<li>Understand the options and guidelines listed in the FDA guideline and USP &lt;621&gt;, &lt;1224&gt;</li>
<li>Develop tips and tricks that eases the burden of scaling and transferring methods</li>
</ul>
Who Should Attend:
<ul>
<li>Any Method development scientists or anyone involved in optimizing/modernizing methods.</li>
</ul>
<blockquote>
Presenter: Mihir Thakar (Product Manager, Phenomenex)
</blockquote>
<blockquote>
Mihir holds master’s degree in biotechnology. He has over a decade of experience working with various Biopharma companies in characterization of therapeutic peptides, proteins, and Oligonucleotides. 
</blockquote>
<blockquote>
He has been associated with Phenomenex more than 5 years, served in various functions including Business Dev. Manager- Biologics. At present, he has been working as a Global Product Manager- Biopharma segment. 
</blockquote>

Phenomenex: Successful Scaling of Biotherapeutic Characterization Methods

Successful Scaling of Biotherapeutic Characterization Methods

Learn key factors of method scaling and transfer. Understand the options and guidelines listed in the FDA guideline and USP <621>, <1224>. Tips and tricks.

Scaling and Transfer of Biotherapeutic Char. Methods

In this presentation, we will present the relevant chromatography, mass spectrometry and spectroscopy based analytical strategies to confidently detect, quantify and purify oligonucleotides and their related impurities that can help you meet both the challenges of synthetic oligonucleotide assessment, and the productivity needs of your lab.
<blockquote>
Presenter: Christopher M. Colangelo, PhD, MBA (Biopharma Business Development Leader, Americas, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Christopher Colangelo, PhD, MBA is the Agilent Biopharma Business Development Leader for the Americas. Prior to joining Agilent, he was the 5th employee and co-inventor of the 454-sequencing platform. In 2003, Chris joined Yale University as a Research Faculty in where he mentored and supervised a research group producing 60+ peer-reviewed publications and directed core research laboratories for four NIH P30 grants, totaling over $21 million worth of research funds. In 2017, Chris joined Agilent to help customers develop, test, and implement end-to-end biopharma workflows.
</blockquote>

Agilent Technologies: Kick Back and Relax, We Got Your Synthetic Oligo Workflows Covered

Kick Back and Relax, We Got Your Synthetic Oligo Workflows Covered

Relevant chromatography, mass spectrometry and spectroscopy based analytical strategies to confidently detect, quantify and purify oligonucleotides.

LCMS webinars focusing on HPLC - page 36