Ask any analytical chemist which hyphenated technique has become indispensable over the past decade, and the answer will almost certainly be the same: tandem chromatography–mass spectrometry. Whether it is <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis">LC–MS/MS</a> quantifying a novel biologic’s metabolic fate, or <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis">GC–MS/MS</a> chasing per- and polyfluoroalkyl substances (PFAS) down to sub-part-per-trillion levels in municipal water, the combination of chromatographic separation with the specificity of tandem mass detection has become the analytical workhorse of choice across virtually every regulated sector.Below we examine the key forces propelling this expansion, from biopharma pipeline dynamics and PFAS regulatory mandates to AI-driven automation and reshoring of pharmaceutical manufacturing—and explore what each trend means for laboratories managing sample preparation and analytical workflows.<h3>Biopharma: The Engine Room of LC–MS/MS Demand</h3><h4>GLP-1 Agonists and the Metabolomics Gold Rush</h4>The explosive commercial and scientific interest in GLP-1 receptor agonists—<a target="_blank" rel="noopener noreferrer" href="https://www.sepscience.com/advancing-glp-1-peptide-analysis-chromatography-challenges-and-innovations-12163">semaglutide and related peptide therapeutics </a>for diabetes and obesity—has dramatically expanded the analytical demands placed on tandem MS platforms. Discovery programs require precise quantification of peptide drug candidates and endogenous biomarkers across complex biological matrices. These workflows rely on high-sensitivity LC–MS/MS for pharmacokinetic profiling, receptor occupancy studies, and metabolomics characterization. Metabolomics is, in fact, projected to be the fastest-growing application segment in mass spectrometry, driven in part by its growing role in cancer screening, biomarker discovery, and metabolic disease research.I have been spending more time learning about GLP-1s because this is the hot topic that people want to discuss on the Concentrating on <a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL?si=n9GLz9zjSCOrVfXPxFPhng&amp;nd=1&amp;dlsi=52868e027aad4d22">Chromatography podcast</a>. With that said, I was astounded looking at CNBC.com during breakfast this morning to see that <a target="_blank" rel="noopener noreferrer" href="https://www.cnbc.com/2026/03/21/glp-1-diets-restaurants-protein-fiber-weight-loss-drugs.html">one in eight Americans are currently taking one of these drugs such as Ozempic</a>.<h4>Antibody–Drug Conjugates and Gene Therapy: Complexity at Every Step</h4>The ascent of antibody–drug conjugates (ADCs) and gene therapies has introduced a new level of analytical complexity. ADCs blend the target specificity of monoclonal antibodies with the cytotoxic potency of small-molecule payloads—and characterizing the linker chemistry, drug-to-antibody ratio (DAR), and conjugate stability demands high-resolution LC–MS/MS at every stage from discovery through QC release testing. Gene therapy vectors require equally rigorous characterization of impurities, aggregates, and empty/full capsid ratios.This translates into a direct need for low-evaporation, high-recovery concentration methods that preserve labile protein structures and maintain sample integrity prior to LC–MS/MS injection. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">Nitrogen evaporation workflows</a> that minimize oxidative stress and maintain sample temperature are increasingly critical in these pipelines.<h4>Precision Medicine and Multi-Omics Integration</h4>The shift toward precision medicine has accelerated adoption of high-throughput, multi-analyte MS platforms. Proteomics, metabolomics, and lipidomics workflows are converging on a single analytical platform—tandem MS—and generating datasets of a scale and complexity that were unimaginable ten years ago.Make no mistake: the triple quadrupole serves an irreplaceable role in quantitative targeted analysis. At the same time, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=134&amp;manufacturerItems=3">Orbitrap </a>and Q–TOF hybrid platforms are growing rapidly as researchers demand full-scan accurate mass for untargeted discovery.<h3>Environmental Mandates: PFAS Testing Drives Adoption</h3>Environmental testing is something that every person, chemist or not, can understand the importance of. Mounting mandates from the U.S. Environmental Protection Agency and European regulators regarding PFAS—the “forever chemicals” whose extraordinary environmental persistence is matched only by the analytical challenge of detecting them at trace levels—have created immediate, compliance-driven investment in LC–MS/MS infrastructure.<h4>The Regulatory Framework Chemists Need to Know</h4>The EPA has codified a suite of LC–MS/MS-based methods as the analytical standard for PFAS in water and solid matrices:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/epa-533-drying-down-pfas-samples-after-spe">EPA Method 533</a>: Weak anion exchange SPE combined with LC–MS/MS; covers 25 PFAS including short-chain compounds in drinking water.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/sample-concentration-epa-method-537-pfas-determination">EPA Method 537.1</a>: Reversed-phase SPE followed by LC–MS/MS; covers 18 PFAS including the GenX compound, with method detection limits in the low ng/L range.</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/en/solutions/environmental/water-testing/pfas-in-water/us-epa-method-8327?srsltid=AfmBOooCY1pcavj7B1PH-75d3HO4BGDglNlhmyTyrpDY3zvUksMO1dlg">EPA Method 8327</a>: LC–MS/MS for selected PFAS in prepared extracts of aqueous and solid matrices.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/preparing-diverse-sample-matrices-for-epa-method-1633">EPA Draft Method 1633</a>: The emerging benchmark for PFAS in non-aqueous matrices—aqueous, solid, biosolids, and tissue—quantifying up to 40 PFAS.</li></ul>All of these methods designate LC–MS/MS, specifically LC coupled to triple quadrupole or tandem-in-time platforms operating in negative electrospray ionization mode with multiple reaction monitoring (MRM), as the required or recommended analytical technique. PFAS analysis is, at its core, an LC–MS/MS problem.<h4>The Sample Preparation Bottleneck</h4>Chemists who have worked with PFAS methods understand the particular demands they place on sample preparation. The ubiquity of<a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/tackling-pfas-contamination-practical-insights-from-epa-537.1-method-development"> PFAS in laboratory materials</a>—PTFE tubing, some SPE sorbents, standard bench supplies—means that blank contamination control is as analytically demanding as the detection itself. EPA 537.1 requires that sample volumes of approximately 250 mL be extracted and reconstituted in as little as 1 mL, creating a 250:1 concentration factor that can expose even trace contamination in the sample processing chain. The installation of a delay column between the LC pump and autosampler to prevent instrument background interference is now standard practice.This is precisely where robust, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5385">inert nitrogen evaporation </a>systems earn their keep. PFAS workflows that use nitrogen-assisted evaporation to concentrate extracts prior to reconstitution benefit from materials that avoid PTFE and other fluoropolymers that can leach PFAS background—a consideration Organomation addresses in its concentration system designs.The analytical community is also moving beyond targeted PFAS analysis. High-resolution accurate mass (HRAM) spectrometry via Orbitrap technology, paired with non-targeted screening workflows, is gaining traction for the identification of the more than 5,000 currently catalogued PFAS compounds and the novel structures that continue to emerge. LC–MS/MS remains the quantitative gold standard; HRAM provides the discovery layer.<h3>Technological Trends Reshaping the LC–MS/MS Workflow</h3><h4>Artificial Intelligence and Automated Data Interpretation</h4>The datasets generated by modern tandem MS platforms—particularly in omics applications—are too large and too complex for conventional manual review. AI-driven spectral deconvolution, automated peak integration, and AI-assisted hypothesis generation are now embedded in the software suites accompanying leading instruments. <a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/about/newsroom/presrel/2022/17feb-gp22004.html?srsltid=AfmBOop3warRIaEojXM2YyMML6RXfMIDzE-Wm0h-SiKkkocBeeET2m7u">Agilent’s acquisition of Virtual Control’s ACIES </a>software specifically to automate LC–MS and GC–MS data analysis is emblematic of this direction.For laboratory managers, AI integration means smaller specialized teams can operate high-throughput multi-analyte workflows—but it also means that the front-end sample preparation must be even more reproducible, since algorithmic interpretation amplifies inconsistencies introduced upstream.<h4>Online SPE Coupling and “Chromatography Without Walls”</h4>A significant trend is the integration of solid-phase extraction directly into the HPLC flow path—so-called online SPE–HPLC coupling. Systems that combine extraction and chromatographic separation in a single analytical instrument reduce manual transfer steps, minimize the risk of sample loss or contamination, and dramatically increase throughput. For analytes at very low concentrations, online SPE provides the on-column concentration capability that traditional offline preparation achieves with manual evaporation steps.That said, offline sample preparation—including <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">nitrogen-assisted evaporation</a> under controlled conditions, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2407">liquid–liquid extraction</a>, and protein precipitation—retains significant advantages in flexibility, matrix handling, and the ability to process sample volumes that exceed online system capacity. The two approaches are increasingly complementary rather than competitive in well-designed analytical laboratories.<h4>High-Purity Solvents and Reference Materials</h4>As detection limits push further into the femtogram range and regulatory methods demand demonstrable blank performance, the quality of analytical consumables—mobile phase solvents, derivatization reagents, reference standards—has moved from background consideration to front-of-mind. Method validation in regulated environments now routinely requires vendor certification of solvent purity at a level that directly interfaces with instrument performance.<h3>The Ever-Present Need for Sample Evaporation</h3>As LC–MS/MS methods push detection limits lower, the analytical burden shifts increasingly upstream. The quality of the concentration, cleanup, and reconstitution steps preceding injection determines whether the instrument’s theoretical sensitivity is achievable in practice.Several specific developments are particularly relevant:<ul><li>ADC and gene therapy characterization workflows require gentle, low-temperature evaporation that preserves protein structure and prevents aggregation prior to LC–MS/MS. Nitrogen evaporation under controlled conditions is the preferred approach.</li><li>PFAS trace analysis demands evaporation systems constructed from PFAS-inert materials, with rigorous attention to avoiding fluoropolymer surfaces throughout the sample path.</li><li>High-throughput pharmaceutical QC driven by reshoring demands parallel processing capability—multi-position evaporators that maintain consistent flow and temperature across all positions simultaneously.</li><li>Multi-omics and metabolomics workflows generate large sample batches that benefit from automated, walk-away evaporation with programmable endpoints to ensure reproducible final volumes across a cohort.</li></ul>Tandem chromatography–mass spectrometry is not merely growing; it is becoming structurally embedded in the analytical workflows of every major regulated industry. The convergence of biopharma complexity, PFAS regulatory mandates, AI-enabled data analysis, and pharmaceutical reshoring is creating a multi-year demand cycle that will require laboratories to invest not only in instruments, but in the entire sample preparation ecosystem that makes those instruments perform.For analytical chemists, the practical takeaway is this: as mass spectrometry sensitivity continues to advance, the sample preparation step becomes the binding constraint on method performance. Whether you are developing a new PFAS method to meet EPA 1633, characterizing an ADC’s drug-to-antibody ratio, or running a 500-sample metabolomics cohort overnight, the reliability and cleanliness of your concentration and cleanup workflow determines the quality of every data point that follows.&nbsp;

The Continued Rise of Tandem Chromatography–Mass Spectrometry

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

Article

Product

Application

Academy

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Tosoh Bioscience

Separation Science

Axcend

Technology Networks

Phenomenex

C&EN

Waters Corporation

Accelerating Routine Food &amp; Beverage Testing with Optimized Methods
Explore the latest technology and methodology solutions to help you deliver results more easily and efficiently for routine Food and Beverage QC testing for : Sugars, Sugar Substitutes Additives, Vitamins and Amino Acids.
<h3>EPISODE 2 - Sugar Additives Analysis</h3>
<h5>Developing Efficient Methodologies for Sugar Substitute Analysis</h5>
<ul>
<li>Presenter: Carsten Fischer, Ph.D. (Principal Technical Specialist Informatics Product Marketing EMEA - Waters)</li>
</ul>
Sugar taxes and consumer demand for sugar-free versions of popular beverage brands have seen a continued increase in the development of diet and zero sugar products. The concentration and combination of different sweeteners in beverages are key to a product’s characteristic flavor and need to be consistent wherever the product is manufactured. Analyzing sugar substitutes and other ingredients in food and beverages is an important assay to ensure a product is within specification. In this talk, we will explore how Waters separation technologies can support the routine analysis of sweeteners and other ingredients in beverages to ensure high and consistent quality of products.
<h5>Increase your laboratory efficiency with Empower</h5>
<ul>
<li>Presenter: Carsten Fischer, Ph.D. (Principal Technical Specialist Informatics Product Marketing EMEA - Waters)</li>
</ul>
Increase your laboratory efficiency with Empower. Learn how Empower&#39;s system suitability checks and custom calculation fields can help streamline food and beverage testing. Ensure system readiness, simplify workflows, and eliminate transcription errors.
<h5>Three methods of sweeteners: Steviol, Acesulfame, and Sucralose on soda and tea matrices</h5>
<ul>
<li>Presenter: Marie-Laure Riandiere (Laboratory Manager - Antartic)</li>
</ul>
We are pleased to receive a special guest Marie-Laure Riandière from the company Antartic SAS, Mousquetaires group. A great thanks for their participation and collaboration to share their quality control expertise in our webinar. Marie-Laure Riandière and her team will be pleased to present their QC analysis of different sweeteners in beverages with HPLC systems and give some highlights on their methods , and chromatograms examples on various matrices.
<h5>Live Q&amp;A session</h5>
<ul>
<li>Moderator: Cecile Pinto (Food &amp; Environmental Market Development Manage, South Europe - Waters)
</li>
<li>Presenter: Carsten Fischer, Ph.D. (Principal Technical Specialist Informatics Product Marketing EMEA - Waters)
</li>
<li>Presenter: Carsten Fischer, Ph.D. (Principal Technical Specialist Informatics Product Marketing EMEA - Waters)
</li>
<li>Presenter: Marie-Laure Riandiere (Laboratory Manager - Antartic)
</li>
</ul>
<blockquote>
Presenter: Dr. Jinchuan Yang (Principal Scientist, Scientific Operations, Waters Corporation)
</blockquote>
<blockquote>
Dr. Jinchuan Yang is a principal scientist in the Scientific Operations at Waters Corporation. Since he joined Waters in 2002, his roles at Waters have mainly focused on application development, marketing development, as well as support for R&amp;D and training. His main technical expertise is in the food and the environmental analysis areas as well as in material characterization. He volunteers in technical groups at AOAC International and USP and serves as a member of expert panels in these organizations.
</blockquote>
<blockquote>
Presenter: Carsten Fischer, Ph.D. (Principal Technical Specialist Informatics Product Marketing, EMEA, Waters)
</blockquote>
<blockquote>
Carsten Fischer, Ph.D., joined Waters in 2004. After a number of years in the Empower data support group, in business development and in informatics sales, his current role is principal technical specialist in the informatics product marketing group for the EMEA region. He provides internal support for Empower CDS Software and is focused on enterprise informatics, compliance, and data science.
</blockquote>
<blockquote>
Presenter: Marie-Laure Riandiere (Laboratory Manager, Antartic SAS)
</blockquote>
<blockquote>
Marie-Laure Riandiere joined Antartic SAS in 1990 and is Laboratory Manager since 2015
</blockquote>

Waters Corporation: Analysis of Sugar Substitutes in Food and Beverages​

Analysis of Sugar Substitutes in Food and Beverages​

Developing Efficient Methodologies for Sugar Substitute Analysis. Laboratory efficiency with Empower. Three methods of sweeteners...

In extractables studies, LC/UV/MS is commonly used to detect and annotate the non-volatile fraction of compounds. Industry guidance and regulatory requirements indicate an analytical evaluation threshold (AET), above which compounds need to be identified to allow for toxicological risk assessment, requiring estimated quantitation before their identity is known. UV and MS detector response is known to vary for analytes, requiring the use of an uncertainty factor to account for difference in response relative to a reference standard at the AET.
In this webinar, we will discuss the challenges associated with these analyses and present strategies that can be deployed to reduce uncertainty in LC/MS extractable studies, including the combination of the three detection techniques in one system to allow the concurrent measurement of UV absorption, CAD signal, and high resolution-accurate mass (HRAM) mass spectra from one injection.
<h5>By attending this presentation you will learn about:</h5>
<ul>
<li>Ways to decrease analytical uncertainty in quantitation of unknown extractables by LC/MS</li>
<li>How incorporating UV, CAD, and MS in one system provides orthogonal detection based on different properties, increasing compound coverage and annotation confidence</li>
<li>How the universal response of the CAD detector, when used with the Vanquish Duo for inverse gradient system, enables semi-quantitation of unknown compounds against surrogate standards with lower uncertainty</li>
</ul>
<blockquote>
Presenter: Chongming Liu
</blockquote>
<blockquote>
Chongming Liu is currently a Supervisor in the Extractables and Leachables (E&amp;L) team at SGS Health Science in Fairfield, New Jersey, USA. At SGS, his daily responsibility is to assist the team members in performing E&amp;L testing on pharmaceutical containers and medical devices and to elucidate structures for compounds of interest based on high-resolution GC-FID/MS and LC-PDA/MS data. Prior to that, he joined the E&amp;L team at SGS in 2018 as an Instrumentation Chemist. Chongming holds a PhD degree in Analytical Chemistry from Stevens Institute of Technology, in Hoboken, New Jersey and a BSc degree in Chemistry from the University of Science and Technology of China (USTC), in Hefei, China.
</blockquote>
<blockquote>
Presenter: Sven Hackbusch
</blockquote>
<blockquote>
Sven Hackbusch is currently a Senior Application Scientist at Thermo Fisher Scientific, responsible for the development of LC/MS applications related to structural elucidation for and in collaboration with customers in the Pharma/Biopharma markets. He joined Thermo Fisher Scientific in 2017 as an Application Scientist in the LC/MS product demonstration laboratory, focusing on untargeted small molecule applications using Thermo Scientific Orbitrap technology. Sven holds a PhD in Pharmaceutical and Chemical Sciences from the University of the Pacific in Stockton, California, USA and a BSc in Chemistry from the Justus Liebig Universität Gießen in Gießen, Germany.
</blockquote>

Pixabay/Jan Felix Christiansen: Practical strategies to decrease uncertainty in comprehensive extractables and leachables analysis by LC/MS

Practical strategies to decrease uncertainty in comprehensive extractables and leachables analysis by LC/MS

We will discuss the challenges associated with these analyses and present strategies that can be deployed to reduce uncertainty in LC/MS extractable studies.

Comprehensive extractables and leachables analysis by LC/MS

Purification is critical to many scientific workflows. Compounds of interest are rarely found to be the right purity in their native state. Join Waters and Virscidian for this 6-part webinar series to learn the basics of purification and application of purification principles for more advanced workflows.
<h3>PART I | Basics of High Performance Liquid Chromatography Purification</h3>
High Performance Liquid Chromatography (HPLC) is a common and effective technique to isolate a compound(s) of interest from a mixture. In this module, we will discuss factors involved in HPLC purification and scale up considerations.
Learning Objectives: 
<ul>
<li>Understand how to use HPLC purification</li>
<li>Make informed decisions on separation modes</li>
<li>Understand impact of decisions made during purification on larger workflow</li>
</ul>
<blockquote>
Presenter: Darcy Shave (Senior Product Manager, Waters Corporation)
</blockquote>
<blockquote>
Darcy Shave is currently aprincipalproduct manager in the Integrated Systems group atWaters Corporation (Milford, MA, USA), responsible for the purification and hardwareautomation products. Darcy has been with Waters for over 20 years, starting as anapplications chemist in the purification group. From there, he moved into a variety ofbusiness development roles before becoming a product manager for purification softwareabout 10 years ago and more recently for the entire purification portfolio.​
</blockquote>
<blockquote>
​He has a degree in Agricultural Chemistry from the University of Saskatchewan and workedin value added processing of natural products before joining Waters.
</blockquote>

Waters Corporation: Basics of High Performance Liquid Chromatography Purification

Basics of High Performance Liquid Chromatography Purification

We will discuss factors involved in HPLC purification and scale up considerations.

Basics High Performance Liquid Chromatography Purification

Axcend Focus LC Free Webinar

What's New in HPLC?

Axcend Focus LC Free Webinar by Axcend team.

Psychedelics are a diverse group of drugs that are known for their ability to alter consciousness, perception, mood, and thought. Detecting the presence, quantity and quality of these compounds is crucial to research development and involves various analytical tools such as High Performance Liquid Chromatography (HPLC) with optical or mass detectors, and other instruments types.
These analytical tests are performed for a variety of reasons, including product, drug, or safety testing, all of which are subject to regulations and guidelines set by the licensing authorities. Besides the regulations, we face several other challenges with psychedelic analysis, such as the lack of standardized testing methods, difficulties in sample preparation, and analyte stability.
During this webinar we will explore:
<ul>
<li>The testing methods used for psychedelic drug analysis, including the headlining compounds of LSD, psilocybin, and MDMA</li>
<li>Ideas to improve our analytical techniques to ensure the most accurate and reliable testing of psychedelic drugs</li>
</ul>
<blockquote>
Presenter: Markus Roggen, PhD (President &amp; CSO, Delic Labs)
</blockquote>
<blockquote>
Presenter: Callum Teevens (Student, University of Victoria)
</blockquote>

Technology Networks: How Long Is Your Trip? Analyzing the Micros and Heroics of Psychedelics

How Long Is Your Trip? Analyzing the Micros and Heroics of Psychedelics

Detecting the presence, quantity and quality of psychedelics is crucial to research development and involves various analytical tools such as HPLC or LC/MS.

Analyzing the Micros and Heroics of Psychedelics

<h5>Key learning objectives:</h5>
<ul>
<li>What is chiral chromatography, chiral screening, why do it, what are the benefits...</li>
<li>What the chiral screening process looks like</li>
<li>Scope, deliverables, and timelines for chiral screening</li>
<li>How to request chiral screening services</li>
</ul>
<blockquote>
Presenter: Zandra Baja (Applications Scientist)
</blockquote>
<blockquote>
Zandra is an Applications Scientist at PhenoLogix specializing in chiral screening, USP and PhEur applications, and environmental GC-MS applications. Zandra obtained her bachelor&#39;s degree in chemistry from California State University of Long Beach. Prior to joining Phenomenex in 2021, she spent 3 years working as a chemist in the petroleum industry and 8 years as an analytical chemist working with gas chromatography in an environmental air testing laboratory. Zandra has also spent several years in quality assurance management where she was responsible for maintaining ISO/IEC 17025 and TNI Standard laboratory accreditations. 
</blockquote>

Phenomenex: Chiral Chromatography Screening 101

Chiral Chromatography Screening 101

What is chiral chromatography, chiral screening, why do it, what are the benefits.... What the chiral screening process looks like....

Understanding the biophysical state, quality and properties of biopharmaceuticals is essential in product and process development. However, available material, time and resources are often limiting.
Dynamic light scattering (DLS) is an industry standard technique for protein formulation, particle sizing and stability studies. This webinar will illuminate how high-throughput DLS (HT-DLS), performed in low-volume microwell plates, is used to understand aggregation as well as colloidal and thermal stability of therapeutic protein candidates and formulations. Application examples include various studies of freeze-thaw, formulation buffer screening and thermal stability.
Key Learning Objectives:
<ul>
<li>Background and instrumentation for HT-DLS</li>
<li>How DLS is used to study aggregation under applied stresses</li>
<li>How DLS evaluates protein colloidal stability via second virial coefficient A2 and the diffusion interaction parameter kD</li>
</ul>
Who Should Attend:
<ul>
<li>Biopharma product development scientists in need of automated methods for characterizing protein stability</li>
<li>Formulation lab managers seeking to improve productivity with high-throughput DLS instrumentation</li>
</ul>
<blockquote>
Presenter: Felix Gloge, Ph.D. (Field Application Scientist, Wyatt Technology)
</blockquote>
<blockquote>
Presenter: Christian Sieg, Ph.D. (Application Scientist, Wyatt Technology)
</blockquote>
<blockquote>
Presenter: Melissa O&#39;Meara (Forensic Science Consultant, C&amp;EN Media Group)
</blockquote>

C&EN: Analyzing Protein Developability and Stability at High-Throughput

Analyzing Protein Developability and Stability at High-Throughput

How high-throughput DLS (HT-DLS), performed in low-volume microwell plates, is use to understand aggregation, colloidal and thermal stability of protein

Protein Developability and Stability at High-Throughput

Purification is critical to many scientific workflows. Compounds of interest are rarely found to be the right purity in their native state. Join Waters and Virscidian for this 6-part webinar series to learn the basics of purification and application of purification principles for more advanced workflows.
<h3>PART 3 | Things to Consider When Configuring Purification Systems and Informatics</h3>
Preparative chromatography is a separation technique where one or more peaks are collected for use in another experiment or process.
In this module, we will discuss the various hardware options available, from pumps and injectors through detection techniques to fraction collection. We’ll also touch on some of the software options available and look at different schemes for triggering fraction collection. 
Learning Objectives: 
<ul>
<li>Understand various instrument configuration and the impact on purification</li>
<li>Optimize system performance</li>
</ul>
<blockquote>
Presenter: Darcy Shave (Senior Product Manager, Waters Corporation)
</blockquote>
<blockquote>
Darcy Shave is currently aprincipalproduct manager in the Integrated Systems group atWaters Corporation (Milford, MA, USA), responsible for the purification and hardwareautomation products. Darcy has been with Waters for over 20 years, starting as anapplications chemist in the purification group. From there, he moved into a variety ofbusiness development roles before becoming a product manager for purification softwareabout 10 years ago and more recently for the entire purification portfolio.​
</blockquote>
<blockquote>
He has a degree in Agricultural Chemistry from the University of Saskatchewan and workedin value added processing of natural products before joining Waters.
</blockquote>

Waters Corporation: Things to Consider When Configuring Purification Systems and Informatics

Things to Consider When Configuring Purification Systems and Informatics

We will discuss the various hardware options available, from pumps and injectors through detection techniques to fraction collection.

When Configuring Purification Systems and Informatics

On December 1, 2022 a harmonized standard for General Chapter 621 Chromatography was released. This standard incorporates the USP with 2.2.46. Chromatographic Separation Techniques European Pharmacopoeia (EuPh) and 2.01 Liquid Chromatography Japanese Pharmacopeia (JP) texts. Harmonization of these regulatory guidance provides increased method flexibility through the employment of modernized of chromatographic tools without the need for full monograph re-validation.
The extent to which the various parameters of a compendial chromatographic test may be adjusted without fundamentally modifying the pharmacopeial analytical procedures is defined in U.S. Pharmacopeia (USP) General Chapter 621 Chromatography.
In this presentation, we combine the gradient method adjustments described in this chapter to achieve both column and system modernization for the USP monograph separation of antiviral drug abacavir sulfate. 
<h5>Key Learning Objectives:</h5>
<ul>
<li>Learn how to modernize the HPLC column and reduce operation costs using the USP 621 allowable adjustments</li>
<li>Learn how to modernize the LC platform and further reduce operation costs using the USP 621 allowable adjustments</li>
<li>Leverage Empower CDS fields to apply the updated chromatography reporting calculations</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Scientists and lab managers involved with compendial methods with a drive towards modernization, productivity and compliance.</li>
</ul>
<blockquote>
Presenter: Catharine Layton (Principal Scientist, Scientific Operations, Waters Corporation)
</blockquote>

Waters Corporation: Modernizing LC Methods as per Harmonized USP Chapter 621

Modernizing LC Methods as per Harmonized USP Chapter 621

We combine the gradient method adjustments described in this chapter to achieve both column and system modernization for the USP monograph.

Christian Rohrer, Director of Sales &amp; Support – EMEA at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.
The talk introduced various chromatographic methods applied in protein purification such as protein A and L affinity chromatography for antibody-derived molecules, chelate affinity for proteins expressed with His-tag, and ion exchange as a versatile first step for protein purification.
<blockquote>
Presenter: Christian Rohrer (Director of Sales &amp; Support)
</blockquote>

Tosoh Bioscience: Tosoh Tuesday - Unlocking the Secrets of Recombinant Protein Purification: A Beginner's Guide

LCMS webinars focusing on HPLC - page 38

Modernizing LC Methods as per Harmonized USP Chapter 621

Things to Consider When Configuring Purification Systems and Informatics

Unlocking the Secrets of Recombinant Protein Purification: A Beginner's Guide

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Chiral Chromatography Screening 101

Analyzing Protein Developability and Stability at High-Throughput

Basics of High Performance Liquid Chromatography Purification

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How Long Is Your Trip? Analyzing the Micros and Heroics of Psychedelics

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Modernizing LC Methods as per Harmonized USP Chapter 621

Things to Consider When Configuring Purification Systems and Informatics

Unlocking the Secrets of Recombinant Protein Purification: A Beginner's Guide

What's New in HPLC?

Chiral Chromatography Screening 101

Analyzing Protein Developability and Stability at High-Throughput

Basics of High Performance Liquid Chromatography Purification

Practical strategies to decrease uncertainty in comprehensive extractables and leachables analysis by LC/MS

How Long Is Your Trip? Analyzing the Micros and Heroics of Psychedelics

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The Continued Rise of Tandem Chromatography–Mass Spectrometry

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Avoiding Common Syringe Filter Mistakes

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