Ask any analytical chemist which hyphenated technique has become indispensable over the past decade, and the answer will almost certainly be the same: tandem chromatography–mass spectrometry. Whether it is <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis">LC–MS/MS</a> quantifying a novel biologic’s metabolic fate, or <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis">GC–MS/MS</a> chasing per- and polyfluoroalkyl substances (PFAS) down to sub-part-per-trillion levels in municipal water, the combination of chromatographic separation with the specificity of tandem mass detection has become the analytical workhorse of choice across virtually every regulated sector.Below we examine the key forces propelling this expansion, from biopharma pipeline dynamics and PFAS regulatory mandates to AI-driven automation and reshoring of pharmaceutical manufacturing—and explore what each trend means for laboratories managing sample preparation and analytical workflows.<h3>Biopharma: The Engine Room of LC–MS/MS Demand</h3><h4>GLP-1 Agonists and the Metabolomics Gold Rush</h4>The explosive commercial and scientific interest in GLP-1 receptor agonists—<a target="_blank" rel="noopener noreferrer" href="https://www.sepscience.com/advancing-glp-1-peptide-analysis-chromatography-challenges-and-innovations-12163">semaglutide and related peptide therapeutics </a>for diabetes and obesity—has dramatically expanded the analytical demands placed on tandem MS platforms. Discovery programs require precise quantification of peptide drug candidates and endogenous biomarkers across complex biological matrices. These workflows rely on high-sensitivity LC–MS/MS for pharmacokinetic profiling, receptor occupancy studies, and metabolomics characterization. Metabolomics is, in fact, projected to be the fastest-growing application segment in mass spectrometry, driven in part by its growing role in cancer screening, biomarker discovery, and metabolic disease research.I have been spending more time learning about GLP-1s because this is the hot topic that people want to discuss on the Concentrating on <a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL?si=n9GLz9zjSCOrVfXPxFPhng&amp;nd=1&amp;dlsi=52868e027aad4d22">Chromatography podcast</a>. With that said, I was astounded looking at CNBC.com during breakfast this morning to see that <a target="_blank" rel="noopener noreferrer" href="https://www.cnbc.com/2026/03/21/glp-1-diets-restaurants-protein-fiber-weight-loss-drugs.html">one in eight Americans are currently taking one of these drugs such as Ozempic</a>.<h4>Antibody–Drug Conjugates and Gene Therapy: Complexity at Every Step</h4>The ascent of antibody–drug conjugates (ADCs) and gene therapies has introduced a new level of analytical complexity. ADCs blend the target specificity of monoclonal antibodies with the cytotoxic potency of small-molecule payloads—and characterizing the linker chemistry, drug-to-antibody ratio (DAR), and conjugate stability demands high-resolution LC–MS/MS at every stage from discovery through QC release testing. Gene therapy vectors require equally rigorous characterization of impurities, aggregates, and empty/full capsid ratios.This translates into a direct need for low-evaporation, high-recovery concentration methods that preserve labile protein structures and maintain sample integrity prior to LC–MS/MS injection. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">Nitrogen evaporation workflows</a> that minimize oxidative stress and maintain sample temperature are increasingly critical in these pipelines.<h4>Precision Medicine and Multi-Omics Integration</h4>The shift toward precision medicine has accelerated adoption of high-throughput, multi-analyte MS platforms. Proteomics, metabolomics, and lipidomics workflows are converging on a single analytical platform—tandem MS—and generating datasets of a scale and complexity that were unimaginable ten years ago.Make no mistake: the triple quadrupole serves an irreplaceable role in quantitative targeted analysis. At the same time, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=134&amp;manufacturerItems=3">Orbitrap </a>and Q–TOF hybrid platforms are growing rapidly as researchers demand full-scan accurate mass for untargeted discovery.<h3>Environmental Mandates: PFAS Testing Drives Adoption</h3>Environmental testing is something that every person, chemist or not, can understand the importance of. Mounting mandates from the U.S. Environmental Protection Agency and European regulators regarding PFAS—the “forever chemicals” whose extraordinary environmental persistence is matched only by the analytical challenge of detecting them at trace levels—have created immediate, compliance-driven investment in LC–MS/MS infrastructure.<h4>The Regulatory Framework Chemists Need to Know</h4>The EPA has codified a suite of LC–MS/MS-based methods as the analytical standard for PFAS in water and solid matrices:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/epa-533-drying-down-pfas-samples-after-spe">EPA Method 533</a>: Weak anion exchange SPE combined with LC–MS/MS; covers 25 PFAS including short-chain compounds in drinking water.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/sample-concentration-epa-method-537-pfas-determination">EPA Method 537.1</a>: Reversed-phase SPE followed by LC–MS/MS; covers 18 PFAS including the GenX compound, with method detection limits in the low ng/L range.</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/en/solutions/environmental/water-testing/pfas-in-water/us-epa-method-8327?srsltid=AfmBOooCY1pcavj7B1PH-75d3HO4BGDglNlhmyTyrpDY3zvUksMO1dlg">EPA Method 8327</a>: LC–MS/MS for selected PFAS in prepared extracts of aqueous and solid matrices.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/preparing-diverse-sample-matrices-for-epa-method-1633">EPA Draft Method 1633</a>: The emerging benchmark for PFAS in non-aqueous matrices—aqueous, solid, biosolids, and tissue—quantifying up to 40 PFAS.</li></ul>All of these methods designate LC–MS/MS, specifically LC coupled to triple quadrupole or tandem-in-time platforms operating in negative electrospray ionization mode with multiple reaction monitoring (MRM), as the required or recommended analytical technique. PFAS analysis is, at its core, an LC–MS/MS problem.<h4>The Sample Preparation Bottleneck</h4>Chemists who have worked with PFAS methods understand the particular demands they place on sample preparation. The ubiquity of<a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/tackling-pfas-contamination-practical-insights-from-epa-537.1-method-development"> PFAS in laboratory materials</a>—PTFE tubing, some SPE sorbents, standard bench supplies—means that blank contamination control is as analytically demanding as the detection itself. EPA 537.1 requires that sample volumes of approximately 250 mL be extracted and reconstituted in as little as 1 mL, creating a 250:1 concentration factor that can expose even trace contamination in the sample processing chain. The installation of a delay column between the LC pump and autosampler to prevent instrument background interference is now standard practice.This is precisely where robust, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5385">inert nitrogen evaporation </a>systems earn their keep. PFAS workflows that use nitrogen-assisted evaporation to concentrate extracts prior to reconstitution benefit from materials that avoid PTFE and other fluoropolymers that can leach PFAS background—a consideration Organomation addresses in its concentration system designs.The analytical community is also moving beyond targeted PFAS analysis. High-resolution accurate mass (HRAM) spectrometry via Orbitrap technology, paired with non-targeted screening workflows, is gaining traction for the identification of the more than 5,000 currently catalogued PFAS compounds and the novel structures that continue to emerge. LC–MS/MS remains the quantitative gold standard; HRAM provides the discovery layer.<h3>Technological Trends Reshaping the LC–MS/MS Workflow</h3><h4>Artificial Intelligence and Automated Data Interpretation</h4>The datasets generated by modern tandem MS platforms—particularly in omics applications—are too large and too complex for conventional manual review. AI-driven spectral deconvolution, automated peak integration, and AI-assisted hypothesis generation are now embedded in the software suites accompanying leading instruments. <a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/about/newsroom/presrel/2022/17feb-gp22004.html?srsltid=AfmBOop3warRIaEojXM2YyMML6RXfMIDzE-Wm0h-SiKkkocBeeET2m7u">Agilent’s acquisition of Virtual Control’s ACIES </a>software specifically to automate LC–MS and GC–MS data analysis is emblematic of this direction.For laboratory managers, AI integration means smaller specialized teams can operate high-throughput multi-analyte workflows—but it also means that the front-end sample preparation must be even more reproducible, since algorithmic interpretation amplifies inconsistencies introduced upstream.<h4>Online SPE Coupling and “Chromatography Without Walls”</h4>A significant trend is the integration of solid-phase extraction directly into the HPLC flow path—so-called online SPE–HPLC coupling. Systems that combine extraction and chromatographic separation in a single analytical instrument reduce manual transfer steps, minimize the risk of sample loss or contamination, and dramatically increase throughput. For analytes at very low concentrations, online SPE provides the on-column concentration capability that traditional offline preparation achieves with manual evaporation steps.That said, offline sample preparation—including <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">nitrogen-assisted evaporation</a> under controlled conditions, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2407">liquid–liquid extraction</a>, and protein precipitation—retains significant advantages in flexibility, matrix handling, and the ability to process sample volumes that exceed online system capacity. The two approaches are increasingly complementary rather than competitive in well-designed analytical laboratories.<h4>High-Purity Solvents and Reference Materials</h4>As detection limits push further into the femtogram range and regulatory methods demand demonstrable blank performance, the quality of analytical consumables—mobile phase solvents, derivatization reagents, reference standards—has moved from background consideration to front-of-mind. Method validation in regulated environments now routinely requires vendor certification of solvent purity at a level that directly interfaces with instrument performance.<h3>The Ever-Present Need for Sample Evaporation</h3>As LC–MS/MS methods push detection limits lower, the analytical burden shifts increasingly upstream. The quality of the concentration, cleanup, and reconstitution steps preceding injection determines whether the instrument’s theoretical sensitivity is achievable in practice.Several specific developments are particularly relevant:<ul><li>ADC and gene therapy characterization workflows require gentle, low-temperature evaporation that preserves protein structure and prevents aggregation prior to LC–MS/MS. Nitrogen evaporation under controlled conditions is the preferred approach.</li><li>PFAS trace analysis demands evaporation systems constructed from PFAS-inert materials, with rigorous attention to avoiding fluoropolymer surfaces throughout the sample path.</li><li>High-throughput pharmaceutical QC driven by reshoring demands parallel processing capability—multi-position evaporators that maintain consistent flow and temperature across all positions simultaneously.</li><li>Multi-omics and metabolomics workflows generate large sample batches that benefit from automated, walk-away evaporation with programmable endpoints to ensure reproducible final volumes across a cohort.</li></ul>Tandem chromatography–mass spectrometry is not merely growing; it is becoming structurally embedded in the analytical workflows of every major regulated industry. The convergence of biopharma complexity, PFAS regulatory mandates, AI-enabled data analysis, and pharmaceutical reshoring is creating a multi-year demand cycle that will require laboratories to invest not only in instruments, but in the entire sample preparation ecosystem that makes those instruments perform.For analytical chemists, the practical takeaway is this: as mass spectrometry sensitivity continues to advance, the sample preparation step becomes the binding constraint on method performance. Whether you are developing a new PFAS method to meet EPA 1633, characterizing an ADC’s drug-to-antibody ratio, or running a 500-sample metabolomics cohort overnight, the reliability and cleanliness of your concentration and cleanup workflow determines the quality of every data point that follows.&nbsp;

The Continued Rise of Tandem Chromatography–Mass Spectrometry

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

Article

Product

Application

Academy

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Agilent Technologies

Phenomenex

Restek Corporation

SelectScience

C&EN

Shimadzu Corporation

Waters Corporation

Accelerating Routine Food &amp; Beverage Testing with Optimized Methods
Explore the latest technology and methodology solutions to help you deliver results more easily and efficiently for routine Food and Beverage QC testing for : Sugars, Sugar Substitutes Additives, Vitamins and Amino Acids.
<h3>EPISODE 2 - Sugar Additives Analysis</h3>
<h3>Agenda</h3>
<h4>Developing Efficient Methodologies for Sugar Substitute Analysis</h4>
<ul>
<li>Presenter: Dr. Jinchuan Tran (Principal Scientist - Waters​)</li>
</ul>
Sugar taxes and consumer demand for sugar-free versions of popular beverage brands have seen a continued increase in the development of diet and zero sugar products. Analyzing sugar substitutes and other ingredients in food and beverages is an important assay to ensure a product is within specification. The concentration and combination of different sweeteners in beverages are key to a product’s characteristic flavor and need to be consistent wherever the product is manufactured. In this talk, we will explore how Waters separation technologies can support the development of efficient methodologies for the routine analysis of sugar
<h4>Increase your laboratory efficiency with Empower</h4>
Speaker: to be announced
Abstract: Increase your laboratory efficiency with Empower. Learn how Empower&#39;s system suitability checks and custom calculation fields can help streamline food and beverage testing. Ensure system readiness, simplify workflows, and eliminate transcription errors.
Three methods of sweeteners: Steviol, Acesulfame, and Sucralose on soda and tea matrices
<ul>
<li>Presenter: to be announced</li>
</ul>
Abstract: Available soon
<h5>Live Q&amp;A session</h5>
<ul>
<li>Moderator: Cecile Pinto (Food &amp; Environmental Market Development Manager, South Europe - Waters)
</li>
<li>Speaker: Dr. Jinchuan Yang (Principal Scientist - Waters)
</li>
</ul>
<blockquote>
Presenter: Dr. Jinchuan Yang (Principal Scientist, Scientific Operations, Waters Corporation)
</blockquote>
<blockquote>
Dr. Jinchuan Yang is a principal scientist in the Scientific Operations at Waters Corporation. Since he joined Waters in 2002, his roles at Waters have mainly focused on application development, marketing development, as well as support for R&amp;D and training. His main technical expertise is in the food and the environmental analysis areas as well as in material characterization. He volunteers in technical groups at AOAC International and USP and serves as a member of expert panels in these organizations.
</blockquote>

Waters Corporation: Analysis of Sugar Substitutes in Food and Beverages​

Analysis of Sugar Substitutes in Food and Beverages​

Topics: Developing Efficient Methodologies for Sugar Substitute Analysi; Increase your laboratory efficiency with Empower.

In recent years, there has been a growing movement to promote alternative methods to animal testing and the EU and a few other countries have even moved to ban the import and sale of cosmetics that have been tested on animals. The Amino acid Derivative Reactivity Assay (ADRA) Method developed by Fujifilm, is an innovative alternative test method for skin sensitization without the use of animals and it was adopted by the OECD in 2022 as a reliable test method.
In this webinar, we will present the technical background of the ADRA method, the use of reagent kits to perform ADRA testing, and our solutions for ADRA analysis that deliver both enhanced selectivity and high-speed testing. Register for this webinar and learn more about how the ADRA method will help you better test the safety of cosmetics and pharmaceuticals. 
<h5>Key Learning Objectives:</h5>
<ul>
<li>Outline of the OECD TG442C (ADRA: Amino acid Derivative Reactivity Assay) </li>
<li>Overview of ADRA kits and related products </li>
<li>How to conduct ADRA testing using HPLC</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>R&amp;D and Manufacturer of Cosmetics </li>
<li>Companies considering Alternative Methods to Animal Testing </li>
<li>Industries interested in Alternative Methods to Animal Testing</li>
</ul>
<blockquote>
Presenter: Toshihiko Kasahara, Ph.D. (Safety Eval. Center, ESG Div., Senior Expert, Fujifilm Corporation)
</blockquote>
<blockquote>
Presenter: Suguru Kitagawa (International Sales, EU Region, Fujifilm Wako Pure Chemical Corporation)
</blockquote>
<blockquote>
Presenter: Ayako Nomura (Analytical Instr. Div., Application Specialist, Shimadzu Corporation)
</blockquote>
<blockquote>
Presenter: Melissa O&#39;Meara (Forensic Science Consultant, C&amp;EN Media Group)
</blockquote>

Shimadzu: The Newest Alternative to Animal Testing for Skin Sensitization

The Newest Alternative to Animal Testing for Skin Sensitization

In this webinar, we will present the technical background of the ADRA method and how to conduct ADRA testing using HPLC.

The Alternative to Animal Testing for Skin Sensitization

<h5>This series will focus on:</h5>
<ul>
<li>An overview on how to ensure your LC system is performing optimally as well as the three main mechanisms that are available to adjust the selectivity of a separation</li>
<li>Allowable changes related to USP&lt;621&gt; with highlights on cost and time savings when modernizing your method</li>
<li>Column chemistry suggestions and tips on how to choose the appropriate column during method development</li>
</ul>
<h5>Who should attend?</h5>
Laboratory managers and scientists who would like to understand how method modernization can save you time and money, those who want to reduce method development time, and those who are curious about how to bring new technology into their laboratory.
<h3>PART 2: USP MONOGRAPHS: HOW TO MODERNIZE METHODS AND STILL BE IN COMPLIANCE</h3>
<ul>
<li>Review the allowable changes to USP monographs for gradient and isocratic assays</li>
<li>Examples of modernized assays from HPLC to UHPLC or UPLC</li>
</ul>
<blockquote>
Presenter: Kenneth Berthelette (Senior Scientist, Waters Corporation Chemistry and Consumables Group)
</blockquote>
<blockquote>
Ken earned his Bachelor&#39;s of Science degree in Professional Chemistry from Bridgewater State University, Bridgewater Massachusetts, in 2010. He has been with Waters Corporation since March 2012 and has worked on several product launches including CORTECS columns, UPC2 Torus and Trefoil columns, and the Atlantis Premier BEH Z-HILIC column. He changed job functions in 2021 and now supports Chemistry Aftermarket initiatives. His focus on applications and customer education has led to several seminars and webinars on topics including method development, method modernization and transfer, HILIC separation techniques, and LC system troubleshooting.
</blockquote>

Waters Corporation: USP MONOGRAPHS: HOW TO MODERNIZE METHODS AND STILL BE IN COMPLIANCE

USP MONOGRAPHS: HOW TO MODERNIZE METHODS AND STILL BE IN COMPLIANCE

Review the allowable changes to USP monographs for gradient and isocratic assays. Examples of modernized assays from HPLC to UHPLC or UPLC.

USP: HOW TO MODERNIZE METHODS AND STILL BE IN COMPLIANCE

Today’s analytical lab is busier than ever, regularly being asked to do more with less. In the name of efficiency, we are advised to “not reinvent the wheel” and simply transfer an existing method from a collaborative lab or a publication. Yet, how often do we attempt this and generate resulting data that is far different from our colleagues or the authors of the referenced article?
In this talk, we will discuss the factors that impact the fidelity of method transfer. We will look at how to measure and compensate for differences in these key system attributes.
<blockquote>
Presenter: Paul Altiero (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Paul Altiero is an Applications Engineer located at the Agilent Little Falls site in Wilmington, DE. Prior to coming to Agilent in 2009, Paul worked in the pharmaceutical industry performing LCMS bioanalysis supporting Drug Discovery DMPK. While at Agilent, Paul has been a field service engineer for LCMS as well as the integrating program manager for the RapidFire high throughput LCMS system. In his current role Paul’s responsibilities include technical support and application assistance for LCMS consumables.
</blockquote>

Why Won’t My HPLC Method Transfer? How to Build a More Robust Method

In this talk, we will discuss the factors that impact the fidelity of HPLC method transfer.

How to Build a More Robust HPLC Method

With a size comparable to that of viruses and self-adjuvating properties, outer membrane vesicles (OMVs) represent an excellent alternative to traditional carrier proteins for conjugate vaccines. An essential aspect of developing OMVs as vaccine products is characterization with respect to size and purity. Asymmetric-flow field-flow fractionation coupled with multi-angle light scattering (AF4-MALS) is an invaluable technique for bionanoparticle analysis, with an AF4-MALS method for quantifying OMV critical quality attributes recently being published in Analytical Chemistry.
This webinar will introduce AF4-MALS and OMV, and review the validation program that was established to assess the quality attributes obtained by AF4-MALS according to the principles described in ICH Guidelines Q2 (R1).
<h5>Key learning objectives</h5>
<ul>
<li>Understand the principles and technology of AF4-MALS</li>
<li>Discover the strategies and parameters for AF4-MALS method development</li>
<li>Learn how to assess OMV quality attributes by AF4-MALS</li>
<li>Consider AF4-MALS validation using ICH guidelines</li>
</ul>
<h5>Who should attend?</h5>
<ul>
<li>Scientists developing analytical methods for bionanoparticles and vaccines</li>
<li>Vaccine development scientists in need of robust, reliable, methods to characterize size and purity</li>
</ul>
<h5>Certificate of attendance</h5>
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Robert van der Put (Intravacc B.V.)
</blockquote>
<blockquote>
Robert van der Put, MSc., is a conjugate vaccine development scientist at Intravacc where he has worked on the development of an OMV-long-peptide vaccine targeting COVID-19 and a peptide conjugate vaccine against ALS. He is involved in the transfer of a Hib conjugate vaccine production process to partners in Asia and has done both in-house and on-site training of international partners for vaccine production, conjugation, purification and quality control testing. Robert is currently studying at the University of Utrecht for a PhD, whilst holding the position of Director of Business Development at Intravacc.
</blockquote>
<blockquote>
Presenter: Roger Scherrers, Ph.D. (Wyatt Technology)
</blockquote>
<blockquote>
Dr. Roger Scherrers studied chemistry at the Heinrich-Heine University in Düsseldorf, where he also completed his PhD. Roger has been with Wyatt Technology Europe since 2007 where he is head of the Application Laboratory. Robert specializes in solving analytical challenges for macromolecules and nanoparticles using light scattering in combination with size-based separation methods such as SEC (size-exclusion chromatography) and FFF (field-flow fractionation).
</blockquote>
<blockquote>
Presenter: Jemima Arnold (Editorial Team, SelectScience)
</blockquote>
<blockquote>
Jemima studied Cellular and Molecular Medicine at the University of Bristol, UK, and as a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
</blockquote>

SelectScience: Characterizing critical quality attributes of outer membrane vesicles for conjugate vaccines

Characterizing critical quality attributes of outer membrane vesicles for conjugate vaccines

We will introduce AF4-MALS and OMV, and review the validation program according to the principles described in ICH Guidelines Q2 (R1).

Characterizing critical quality attributes of vaccines

The emergence of NPS (novel psychoactive substances) on the illicit drug market has created a challenge for toxicology laboratories. With the constant rise of novel compounds, keeping a laboratory testing scope up-to-date is proving increasingly difficult.
This webinar will provide a background on NPS as well as strategies and tools, such as Restek’s Pro EZLC Chromatogram Modeler, that can be used to effectively add NPS compounds to a laboratory’s testing scope using Restek’s Raptor Biphenyl column.
<ul>
<li>Learn key information on how to effectively analyze NPS (novel psychoactive substances) compounds.</li>
<li>See how the Pro EZLC Chromatogram Modeler can help with adding these compounds to your analysis.</li>
<li>Learn how Raptor Biphenyl allows for easy incorporation of these compounds into existing methods.</li>
</ul>
<blockquote>
Presenter: Haley Berkland (LC Applications Scientist)
</blockquote>

Restek Corporation: Strategies for Adding Xylazine, Nitazenes, and Other NPS to Laboratory Testing Scopes

Strategies for Adding Xylazine, Nitazenes, and Other NPS to Laboratory Testing Scopes

We will provide a background on NPS as well as strategies and tools, such as Restek’s Pro EZLC Chromatogram Modeler...

Strategies for Adding Xylazine, Nitazenes, and Other NPS

Welcome to the &quot;Ultra-Fast Bites&quot; series - where you can get ultra-fast insights about the latest trends and hottest topics in bite-sized content to stay at the forefront of science.
In our previous For the Love of Food! series, we have previously discussed the use of LC-GC-FID, which is the industry standard for monitoring mineral oil pollutants in edible oils and food contact materials.
These pollutants are frequently encountered as saturated (MOSH) and aromatic hydrocarbons (MOAH), making it essential to analyze for food safety. However, with the analytical challenges, additional sample clean-up procedures (AlOx and Epox) must be used to improve the method&#39;s robustness and sensitivity.
In this presentation, join us as Dr. Andreas, Managing Director of Axel Semrau GmbH &amp; Co KG, introduces a fast and efficient analysis of MOSH/MOAH using a new automated saponification workflow that can save your time and effort while maximizing the results.
For the love of food!
Learning Objectives:
<ul>
<li>Learn more about LC-GC-FID, which is the standard instrumentation to monitor the levels of MOSH/MOAH</li>
<li>Understand automated clean-up techniques for the analysis of MOSH/MOAH samples</li>
<li>Discover how the new automated saponification workflow can help save your time and effort in analysis</li>
</ul>
<blockquote>
Presenter: Dr Andreas Bruchmann (Managing Director, Axel Semrau GmbH &amp; Co KG)
</blockquote>
<blockquote>
After studying Analytical Chemistry at the University of Bochum, Andreas Bruchmann joined Axel Semrau in 1992. He worked in different departments before becoming Managing Director in 2010. Now he is responsible for the Chromatography business unit, which supplies automated application solutions for the food and pharmaceutical industry worldwide. He was also involved in the development of the CHRONECT MOSH/MOAH Workstation from the beginning in 2010.
</blockquote>

Shimadzu Corporation: Ultra-Fast Bites - For the Love of Food! Latest Update on the Analysis of MOSH/MOAH Contamination

Latest Update on the Analysis of MOSH/MOAH Contamination

A fast and efficient analysis of MOSH/MOAH using a new automated saponification workflow that can save your time and effort while maximizing the results.

<h5>Key Learning Points:</h5>
<ul>
<li>Learn how to prevent and minimize problems before they occur</li>
<li>Understand how to approach problem solving and troubleshooting in a logical way, including: diagnose cause and possible solutions</li>
<li>Review peak shape, and column performance problems</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Any LC users who would like to get tips on extend the column lifetime</li>
</ul>
<blockquote>
Presenter: Kevin Zhao (Senior Technical Specialist, Phenomenex)
</blockquote>

HPLC Troubleshooting

Learn how to prevent and minimize problems before they occur. Understand how to approach problem solving and troubleshooting. Review peak shape.

For years HPLC is one of the golden standards in analytical chemistry. Commercially available systems have been available since the 1970s, and modern systems started in the 1990s and 2000s to dominate analytical labs e.g. in quality control or in research and development.
Today many running systems are 10 or 15 years old, sometimes even older. What are the pitfalls and risks when working on these systems and how can you use these systems at their best?
This webinar will give a short overview about the possibilities to keep an aged system running, to upgrade it or to exchange it to modern UHPLC systems.
<blockquote>
Presenter: Dr. Jens Trafkowski (Produktspezialist HPLC, Agilent Technologies)
</blockquote>
<blockquote>
Jens Trafkowski ist seit 2017 Produktspezialist für HPLC bei Agilent Technologies, nachdem er zuvor mehr als 5 Jahre als globaler Produktmanager für verschiedene HPLC-Systeme und -Produkte tätig war, vor allem bekannt für seine Verantwortung für die 1290 Infinity II 2D-LC. Vor seinem Wechsel zu Agilent Technologies im Jahr 2011 war er mehr als 6 Jahre als Applikationsspezialist für LC-MS tätig.
</blockquote>
<blockquote>
Nach seinem Studium der Lebensmittelchemie an der Universität Bonn promovierte er am Institut für Rechtsmedizin Bonn mit einer Arbeit über Anwendungen der HPLC-MS/MS in der forensischen und klinischen Toxikologie.
</blockquote>
<blockquote>
Jens Trafkowski has been Product Specialist for HPLC at Agilent Technologies in Basel since 2017, having previously worked as Product Manager at the development site in Waldbronn, responsible among other things for the analytical HPLC pumps, but primarily for the 1290 Infinity II 2D-LC Solution.
</blockquote>
<blockquote>
Before joining Agilent Technologies in 2011, he was an application specialist for LC-MS for more than six years, responsible for training, technical support, and application development. After studying food chemistry, Jens Trafkowski focused on the analysis of drugs and pharmaceuticals in human samples and completed his PhD at the Institute of Legal Medicine in Bonn on applications of HPLC-MS/MS in forensic and clinical toxicology.
</blockquote>

Agilent Technologies: HPLC systems running out of support - how can you handle?

HPLC systems running out of support - how can you handle?

This webinar will give a short overview about the possibilities to keep an aged system running, to upgrade it or to exchange it to modern UHPLC systems.

Integration is essential to chromatography for the identification and determination of peak area and height.
Although Software does an excellent job with integration, chromatography is not always perfect and careful consider must be given to integration.
In this presentation, three essential integration topics are covered: Integration parameters--some basic parameters must be understood when reviewing chromatography to ensure optimal analyses. Integration techniques--the most appropriate for solving some common problems are discussed. Finally, manual intervention--given the overall goal to achieve robust integration, when and why manual integration should be performed.
<blockquote>
Presenter: Greg Hunlen (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Gregory Hunlen has served as an Application Scientist with Agilent Technologies since 2012. He applies his expertise to the Agilent Infinity II series of LC instruments, providing knowledge of LC solutions such as two dimensional LC and LCMSD workflows. He also delivers product demonstrations and lunch &amp; learns. As a key value, Greg likes to talk about maximizing the use of all features our technologies provide. Often, instruments are under-utilized perhaps as a lack of understanding of all that they can offer. When we explore these features, we discover that we can truly optimize the efficiencies of our instruments. Prior to joining Agilent, Greg spent 20 + years in the pharmaceutical research industry on the liquid phase chromatography side of drug development.
</blockquote>

Agilent Technologies: HPLC Integrate with Confidence

HPLC Integrate with Confidence

Integration parameters--some basic parameters must be understood when reviewing chromatography to ensure optimal analyses.

LCMS webinars focusing on HPLC - page 39

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Characterizing critical quality attributes of outer membrane vesicles for conjugate vaccines

Why Won’t My HPLC Method Transfer? How to Build a More Robust Method

Latest Update on the Analysis of MOSH/MOAH Contamination

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HPLC Integrate with Confidence

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HPLC systems running out of support - how can you handle?

Strategies for Adding Xylazine, Nitazenes, and Other NPS to Laboratory Testing Scopes

Characterizing critical quality attributes of outer membrane vesicles for conjugate vaccines

Why Won’t My HPLC Method Transfer? How to Build a More Robust Method

Latest Update on the Analysis of MOSH/MOAH Contamination

HPLC Troubleshooting

HPLC Integrate with Confidence

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Related content

The Continued Rise of Tandem Chromatography–Mass Spectrometry

News from LabRulezLCMS Library - Week 27, 2026

Avoiding Common Syringe Filter Mistakes

Analysis of Sugar Substitutes in Food and Beverages