Meeting EU Drinking Water Standards: The Critical Role of Sample Prep in Trace Contaminant Analysis

<h3>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</h3>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711">Among the analytical techniques available today, anion exchange chromatography (AEX) stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</a></blockquote><h4>How Scientists Read Oligonucleotides Using Charge</h4>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.<h4>Why Ion Exchange Works: Binding, Retention, and Release</h4>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, anion exchange is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.The system consists of two main components:<ul><li>Stationary phase: A positively charged resin that binds anionic analytes.</li><li>Mobile phase: An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"><h4>The Five-Step Workflow of Ion Exchange Chromatography</h4>AEX separations generally follow a well-established sequence of steps:<ol><li>Column equilibration The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li>Sample loading Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li>Washing The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li>Elution Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li>Regeneration and re-equilibration A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2 Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"><h4>Why AEX Is Especially Effective for Oligonucleotides</h4>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.Key factors influencing AEX behavior include:<ul><li>Charge density: Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li>Resin chemistry: Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li>Buffer composition: The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"><h4>AEX as the Gold Standard for Oligonucleotide Separation</h4>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:<ul><li>High resolving power: Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li>Predictable retention behavior: In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li>Compatibility with modified oligonucleotides: Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li>Excellent control via gradient elution: Salt gradients provide precise tuning of selectivity and resolution.</li></ul>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"><h4>Final Perspective: Mastering AEX with Confidence</h4>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.<blockquote>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at sales@knauer.net. More insights into practical oligonucleotide analysis will follow in our “Oligos Made Easy” series.For technical questions or in-depth discussion, the author can be reached at kindler@knauer.net</blockquote>

Oligos Made Easy - Part 2: AEX

Although modern agriculture needs to be based on accurate real-time data on soil, plant, and environmental conditions, affordable, sufficiently sensitive, and robust monitoring devices for this purpose are currently missing. According to scientists, low-cost sensors based on new smart materials and printing technologies offer a suitable solution. The results of multidisciplinary research thus far and proposals for the development of these devices have been presented by researchers from Czechia, Great Britain, Spain, and the USA in a review article in the prestigious journal <a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-68778-6#Abs1">Nature Communications</a>.“Sensor technologies for monitoring plant health are still in their infancy. Only simple sensors capable of monitoring basic parameters such as pH, soil moisture, or temperature are commonly available on the market. However, if farmers are to face current challenges, including climate change, and ensure sufficient food for humanity, they need much more effective tools that will enable them to monitor, for example, various pathogens, including bacteria and fungi. In our opinion, a suitable solution is a combination of new smart materials and printing for the production of inexpensive, durable, and scalable materials,” said the article’s first author, David Panáček from CATRIN, who worked on the study during his internship at Imperial College London.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg" alt="CATRIN: David Panáček" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg 208w," sizes="100vw" width="1536" height="1152">Sensors for monitoring plant health, for example, lag behind similar devices used in human medicine. According to the authors, advances in materials science, which will enable the development of scalable sensor technologies, play a key role in this potential shift. “Printing techniques, including inkjet printing, screen printing, aerosol printing, 3D printing, and direct laser writing, offer flexible ways to produce flexible sensors, large-area sensors, and sensors integrated directly into plants. The review article summarizes recent advances in printable low-dimensional materials for agricultural sensors, analyzes their physico-chemical properties in relation to sensor performance, and discusses key challenges and future opportunities requiring interdisciplinary integration,” said one of the corresponding authors, Michal Otyepka.The research brought together materials scientists, chemists, biologists, sensing experts, and printing technology specialists. Materials research and the use of graphene-based smart materials were led by scientists from CATRIN at Palacký University and VŠB-Technical University of Ostrava. Plant biologists from CATRIN contributed their knowledge of plant development, their responses to stress conditions, and the monitoring of their properties. Researchers from Imperial College London took care of the electronics, while representatives from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/36"> CEITEC – VUT</a> focused on printing technologies. The team also included leading experts in sensing from the Catalan Institute of Nanoscience and Nanotechnology in Barcelona, and Joseph Wang of the University of California San Diego, the “father of sensing and electrochemistry,” also contributed his extensive knowledge.“This is a groundbreaking study on this topic, which has managed to attract some truly big names in their respective fields. Last but not least, the study demonstrates that interdisciplinarity at CATRIN gives rise to completely new and promising research topics, such as plant sensing and its use for studying plant-environment interactions and smart agriculture,” added another corresponding author, Nuria De Diego.

Current sensors are not good enough. Scientists propose a new way to monitor plant health

Article

Environment

Scientific article

Science and research

Video

The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Metrohm

Bruker

Phenomenex

Thermo Fisher Scientific

Wiley

SCIEX

Waters Corporation

Per- and poly-fluorinated substances (PFAS) are synthetic organic compounds widely used in products and processes because of their hydrophobic and non-stick properties. Known as ‘forever chemicals’ due to their persistence in the environment, PFAS are identified as a class of environmental contaminants of high concern. Increasingly, environmental and regulatory agencies are taking action to address PFAS contamination, setting sub-parts-per-billion (ppb) tolerance thresholds to limit exposure and protect public health.
While targeted techniques can detect these compounds at low levels, the number of current targets is limited. Consequently, the use of non-targeted High Resolution Mass Spectrometry (HRMS) is becoming important for the analysis of PFAS. This is due to the rapid rate of detection of novel PFAS in samples and a lack of analytical standards.
Join us for these short two-part series (30 min) to take your PFAS analysis to the next level, where we will demonstrate the benefit of using HRMS workflows for advanced discovery, identification and quantitation of PFAS. 
Learn about recent LC developments and how the Xevo™ G3 QTof, and a SELECT SERIES™ Cyclic™ Ion Mobility Spectrometry (IMS) rise to this analytical challenge. 
Gain practical insights on workflows used by our scientists for your own PFAS investigations; see the range of inlet options available, how to run samples, experience the latest software for data acquisition and interpretation.
Register for:
Part 1 – 16th May (10:30-11:00 BST | 11:30-12:00 CEST)
LC developments and using Xevo G3 QTof for quantitation and screening of PFAS 
Part 2 – 23rd May (10:30- 11:00 BST | 11:30-12:00 CEST)
Advanced non-target approaches using Ion mobility
Key learning topics:
<ul>
<li>Recent developments in LC and why selection of column chemistries is critical for PFAS analysis </li>
<li>Learn about HRMS and IMS in the quest to discover new PFAS compounds and enhance confidence in identification of PFAS </li>
<li>See our chemists in action in the lab and gain practical insights on workflows</li>
</ul>
Who should attend?
<ul>
<li>Researchers and scientists actively addressing the PFAS issue using Liquid Chromatography or Mass Spectrometry.</li>
<li>PFAS Program Managers</li>
<li>Laboratory Managers</li>
</ul>
<blockquote>
Presenter: Stuart Adams (Consulting Scientist, Testing &amp; Lab Services, Food and Environmental, Waters Corporation)
</blockquote>
<blockquote>
Stuart started his career by attending the University of Glasgow studying for an honours degree in Food and Environmental Chemistry where his passion for solving analytical challenges started. He furthered his education with a doctorate from the University of Leeds in analytical chemist. Subsequently he spent 17 years working at Fera on a variety of projects looking at the detection of chemicals in challenging food and environmental samples using a variety of detection techniques. Whilst at Fera he has represented the UK at several European Union Reference Laboratory meetings for pesticide analysis and is a recognised international expert in his field. Stuart joined Waters Corporation in 2018 and currently works as a Consulting Scientist where he still loves to work on solving the analytical challenges that matter.
</blockquote>
<blockquote>
Presenter: Dr. Hania Khoury-Hollins (Principal Scientist, Waters Corporation)
</blockquote>
<blockquote>
Hania Khoury-Hollins is a scientist and researcher at Waters Corporation. She holds a bachelor’s degree in science, which laid the foundation for her scientific pursuits. In 2001, Hania earned her master’s degree in Toxicology, deepening her understanding of the impact of chemicals on living organisms. In 2005 she successfully defended her Ph.D. in physical chemistry from University René Descartes - Paris V (France). After undertaking several different projects in academia Hania spent some time working at Agilent before joining Waters.
</blockquote>
<blockquote>
At Waters, her work revolves around developing cutting-edge analytical techniques and their applications with a focus on Liquid Chromatography Coupled with High-Resolution Mass Spectrometry (LC-HRMS). Her expertise encompasses a diverse range of applications, including drug development, proteomics, metabolomics, and lipidomics. More recently, Hania has been developing applications strategies for per- and polyfluoroalkyl substances (PFAS).
</blockquote>

Waters Corporation: Take your PFAS Analysis to the Next Level (1) with Advanced MS for Non-Targeted Analysis

LC developments and using Xevo G3 QTof for quantitation and screening of PFAS

Learn about HRMS and IMS in the quest to discover new PFAS compounds and enhance confidence in identification of PFAS.

LC developments and using Xevo G3 QTof for PFAS

Waters Corporation: Take your PFAS Analysis to the Next Level (2) with Advanced MS for Non-Targeted Analysis

Advanced non-target approaches (PFAS) using Ion mobility

Learn about HRMS and IMS in the quest to discover new PFAS compounds and enhance confidence in identification of PFAS

Recognizing the importance of cellular metabolism and the complexity of cell-cell heterogeneity, SpaceM has emerged as a promising technology with high potential for investigating single-cell metabolomics. SpaceM is expected to grant broad access to single-cell metabolomics, enabling investigations across diverse cellular models. Additionally, addressing the challenge of reproducibility, we present HT-SpaceM, a high-throughput method showcasing robust reproducibility in identifying metabolites in 10 cell lines, highlighting the promise of the SpaceM method in understanding cellular metabolism and disease.
<blockquote>
Presenter: Shawn Owens, Ph.D. (Imaging Mass Spectrometrist, SpaceM, BioInnovation Institute, Copenhagen, Denmark)
</blockquote>
<blockquote>
Shawn began his mass spectrometry imaging journey over 10 years ago, at the University of California- Santa Barbara- where he earned his PhD. With his degree in hand, he began a journey that led him through roles in industry- first at Bruker for nearly 5 years, followed by a year at BASF in New York. Eager to explore new horizons, Shawn embarked on a transformative journey in a startup, joining SpaceM in Copenhagen, Denmark- where he assumed the role of R&amp;D Team Leader and Mass Spectrometrist, a role he has held for the past 18 months.
</blockquote>

Bruker: High Throughput Single-Cell Metabolomics

High Throughput Single-Cell Metabolomics

We present HT-SpaceM, a high-throughput method showcasing robust reproducibility in identifying metabolites in 10 cell lines.

One of the key aspects of water quality assurance is monitoring disinfection byproducts (DBPs), especially haloacetic acids (HAAs), bromate, and dalapon.
As these substances pose a significant health risk, regulatory bodies across the world, such as the US EPA and the European Union, have established strict guidelines to limit their presence in drinking water. The EPA Method 557 by the US EPA standardizes the comprehensive monitoring of 9 HAAs, bromate, and dalapon.
This webinar will discuss the latest advancements in analytical techniques and instrumentation that meet the regulatory requirements. The focus will be on exploring the capabilities of ion chromatography coupled with mass spectrometry (IC-MS and IC-MS/MS), a method that is highly sensitive, reliable, and efficient in detecting low levels of HAAs and other DBPs without requiring extensive sample preparation.
Attendees will have the opportunity to witness the seamless integration of HAA analysis by IC-ESI-MS/MS and IC-ESI-MS within water laboratories, demonstrating consistent results and efficient lab processes. Our experts will showcase how ion chromatography and mass spectrometry provide a streamlined workflow, from sample injection to analysis, significantly reducing manual labor and associated costs. 
<h5>Key learnings of this webinar:</h5>
<ul>
<li>Learn how IC coupled to mass spectrometry is the ideal and most reliable technique for analysis of HAA, bromate, and dalapon in drinking water</li>
<li>Develop an understanding of how ion chromatography and mass spectrometry can complement each other to enhance selectivity and sensitivity</li>
<li>Learn to know Metrohm and Agilent as your reliable lab partners</li>
</ul>
<h5>Who should attend?</h5>
<ul>
<li>Water utility operators who need robust and dependable analytical techniques to guarantee the safety of drinking water</li>
<li>Scientists, researchers, and analytical method developers who need to stay up-to date on state-of-the-art methods for water analysis</li>
<li>Laboratory staff and managers considering to implement reliable, cost- and time-efficient analysis of HAA in their lab</li>
</ul>

Metrohm: Water safety: Improve your analysis of haloacetic acids with IC-ESI-MS/MS

Water safety: Improve your analysis of haloacetic acids with IC-ESI-MS/MS

The latest advancements in analytical techniques that meet the regulatory requirements in monitoring disinfection byproducts (DBPs).

Improve your analysis of haloacetic acids with IC-ESI-MS/MS

<h5>Overview:</h5>
<ul>
<li>The reporting limit of a method is also dependent upon the consistency of clean blanks and elimination of sporadic contamination.</li>
<li>Vendors of PFAS consumables need to provide certified, trace level PFAS free products.</li>
<li>PFAS are everywhere! They are widely used, vendors/suppliers when contacted don&#39;t know their processes are using them.</li>
<li>Since PFAS contamination has become an issue with PFAS analysis all field samples are taken in duplicate, not just field blanks. </li>
<li>The complete data package and replicates need to be evaluated by the user in order to make sound conclusions and identify false positives. </li>
<li>Every step there is a chance to incorporate “free-range” PFAS in your sample, minimize the preparation steps</li>
<li>How PFAS is analyzed at EPA Region 5 Laboratory</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Anyone lab scientists, or managers involved in PFAS Analysis</li>
</ul>
<h5>Key Learning Points:</h5>
<ul>
<li>Learn how to approach analyzing for PFAS, it is not rocket science like they made it out to be</li>
<li>Become aware of Sporadic PFAS Contamination</li>
<li>Understand how to identify false positives</li>
<li>Require all analysis quality control and samples to be analyzed in replicates to identify false positives.</li>
</ul>
<blockquote>
Presenter: Larry Zintek (Organic Methods Group Leader, UA EPA)
</blockquote>
<blockquote>
Presenter: Maryam Sayeed (Product Management Specialist, Phenomenex)
</blockquote>

Phenomenex: Identifying and Avoid False Positives During Routine PFAS Analysis

Identifying and Avoid False Positives During Routine PFAS Analysis

Learn how to approach analyzing for PFAS, it is not rocket science like they made it out to be.

False Positives During Routine PFAS Analysis

<h4>LC-MS/MS fundamentals webinar series 2.0</h4>
Advanced techniques for data acquisition and processing
Elevate your analytical expertise with advanced techniques and workflows
Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series. Based on our LC-MS/MS 1.0 webinar series, these sessions will go beyond the fundamentals of LC-MS/MS and introduce ways to advance your analytical methods and data processing workflows.
Every other week, we will explore how to optimize SCIEX OS software with short, 30-minute expert-led presentations.
<h4>This webinar:</h4>
May 21, 2024 11:00 a.m. - 11:30 a.m. CET - Advanced MS/MS method building 
Gain insight into advanced features for maximizing sensitivity in your mass spectrometry analyses.
<h4>Other webinars from the series:</h4>
May 7, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3820">Beyond the fundamentals</a>
June 4, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3853">Mastering chromatography</a>
Discover how to optimize your chromatography for even the most challenging compounds while creating and building LC methods.
June 18, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3854">It&#39;s a TRAP! Getting the most out of your QTRAP system</a>
Learn when to utilize QTRAP functionality to create more selective and sensitive methods. July 2, 2024
11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3855">Troubleshooting at the source</a>
Acquire the knowledge needed to optimize source parameters and identify and resolve ionization-related problems.
July 16, 2024 11:00 a.m. - 11:30 a.m. CET <a href="https://lcms.labrulez.com/webinar/3856">Automating data processing</a>
Learn how to automate your data processing with custom flagging and rules for efficient data review in SCIEX OS software.
July 30, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3857">Excel strategies for mass spectrometry data analysis</a>
Take an in-depth look at when and how to use Excel to increase your productivity.

SCIEX: LC-MS/MS fundamentals webinar series 2.0

Advanced MS/MS method building

Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series.

The new Thermo Scientific Orbitrap Astral mass spectrometer is setting a new standard for mass spectrometry performance, redefining what is possible and unlocking additional insights into biology and disease mechanisms.
The ability to conduct large cohort protein analysis with faster throughput, deeper coverage, higher sensitivity and accurate and precise quantitation enables a better understanding of the dynamic, temporal and spatial complexities of biology.
In this interactive webinar, we will briefly introduce underlying technology of the Orbitrap Astral mass spectrometry platform before opening the floor to a group of renowned cancer researchers who will describe their own early experiences with the system and their hopes and ambitions for advancing their own researches in this important and rapidly-advancing field.
As a participants in this webinar, you will have the opportunity to engage in an interactive panel discussion with these key opinion leaders and ask questions to further enhance your understanding of the current and future state of mass spectrometry-driven proteome analysis of cancer samples and how that can identify differentially expressed proteins, protein-protein interactions and post-translational modifications that may be associated with cancer development, progression, or response to treatment.
What you will learn:
<ul>
<li>View the latest research in protein profiling</li>
<li>Learn about the Orbitrap mass spectrometry solutions</li>
<li>Discover how your cancer research can be empowered</li>
</ul>
Who should attend?
<ul>
<li>Scientific researchers in the field of cancer proteomics</li>
<li>Mass spectrometrists working in core labs in cancer institutes</li>
<li>Laboratory managers in cancer institutes</li>
</ul>
If you have questions for our experts, please submit them during registrations or send them anytime in advance via email to analyze.eu@thermofisher.com
Live questions will be handled during the webinar in our Q&amp;A chat. 
Short notice: Can&#39;t attend the live broadcast? No problem! Register anyway and you will automatically receive the link to the webinar recording.
<blockquote>
Presenter: Dr. Christian Hock (Senior Manager Instrument Development, Thermo Fisher Scientific)
</blockquote>
<blockquote>
The Orbitrap Astral development team is led by Dr. Christian Hock, who joined the Research team of Dr. Alexander Makarov as Lead Scientist in early 2013. With a strong passion for science and an innate curiosity, Dr. Hock has been involved in all aspects of Astral technology development, from its initial stages of basic research to its successful transition into serial production.
</blockquote>
<blockquote>
In 2017, Dr. Hock transferred to the Bremen R&amp;D organization, where he took the initiative to establish a dedicated R&amp;D team which has been instrumental in bringing the Orbitrap Astral product to fruition. His overarching goal is to exceed customer expectations by delivering cutting-edge products that enable researchers worldwide to accelerate their scientific output. Through his leadership and expertise, he continuously pushes the boundaries of science &amp; technology and strives for excellence in every stage of product development.
</blockquote>
<blockquote>
Presenter: Prof. Michiel Vermeulen (Senior Group Leader, Netherlands Cancer Institure NKI)
</blockquote>
<blockquote>
Presenter: Dr. Harmjan Vos (Head of the Proteomics Facility, University Medical Centre Utrecht)
</blockquote>
<blockquote>
Started his proteomics career as a postdoc in a project of Jeroen Krijgsveld in the lab of Albert Heck at the UU. Then he moved to the lab of Boudewijn Burgering at the Center for Molecular Medicine (CMM) at the UMC Utrecht, trying to understand the intricate connections between cellular signaling pathways and metabolic processes. Since 2014 he is heading the Proteomics facility at CMM, which is involved in the research infrastructure of the Oncode Institute, with an emphasis on single cell proteomics.
</blockquote>
<blockquote>
Presenter: Prof. Jyoti Choudhary (Group Leader, The Institute of Cancer Research)
</blockquote>
<blockquote>
Professor Jyoti Choudhary&#39;s research focuses on the development and implementation of novel mass spectrometry and data analysis approaches for proteome discovery. She is Head of the Proteomics Core Facility and Career Faculty Leader of the Functional Proteomics Research Group.
</blockquote>
<blockquote>
Presenter: Dr. Joanna Kirkpatrick (Technical Sales Specialist, Proteomics, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Jo has a degree in Chemistry from the University of York and a PhD in analytical chemistry from the University of Reading. Following a post-doc in mass spectrometry in the Chemistry department at Oxford, and working as an application scientist at Waters, she has worked in and ran Proteomics Core Facilities in the UK and Germany, including the EMBL in Heidelberg, the Leibniz Institute on Aging in Jena, The Francis Crick Institute in London and CRUK Cambridge.
</blockquote>
<blockquote>
After many years as an experienced Thermo customer, Jo joined the company last year as a Technical Sales Specialist, supporting the whole of the UK/Ireland, Benelux and Nordic regions, in all things proteomics.
</blockquote>
<blockquote>
When she&#39;s not talking about proteomics and mass spectrometry, Jo enjoys ceroc dancing, tennis, scuba diving, travelling, and spending time with her cats, who like to join in on Teams meetings whenever she works from home.
</blockquote>

ThermoFisher Scientific: High throughput protein profiling for cancer research by Orbitrap Astral MS

High throughput protein profiling for cancer research by Orbitrap Astral MS

Cancer research by Orbitrap Astral MS

Since 2018 when the presence of Nitrosamines was first reported in several ARB (angiotensin II receptor blocker) drugs, there has been increasing regulatory focus to mitigate the presence of these potential human carcinogens in drug substances and drug products.
<h5>Who Should Attend:</h5>
<ul>
<li>Anyone with an interest in, or challenged by, the analysis N-Nitrosamines and Nitrosamine Drug Substance Related Impurities (NDSRI) in API and/or Drug Products
</li>
<li>Analysts and Scientists working in the Pharmaceutical and Generic Pharmaceutical industries.
</li>
</ul>
<h5>Key Learning Points:</h5>
<ul>
<li>Review the latest regulatory updates (FDA and EMA)
</li>
<li>Learn about published LC-MS/MS applications which address the N-Nitrosamines and NDSRI analysis challenges in drug products
</li>
<li>Find solutions to sample preparation challenges for N-Nitrosamines analysis in drug products
</li>
<li>Obtain examples of specific Phenomenex applications for nitrosamines (10 mutagenic nitrosamine impurities in Acyclovir) and NDSRIs (N-nitroso propranolol in a propranolol drug substance and product)
</li>
</ul>
<blockquote>
Presenter: Phil Koerner (Market Development Manager, Pharmaceuticals)
</blockquote>

Phenomenex: Nitrosamines in Drug Products: Where Are We Now?

Nitrosamines in Drug Products: Where Are We Now?

Learn about published LC-MS/MS applications which address the N-Nitrosamines and NDSRI analysis challenges in drug products

<h4>Virtual event: Pharma conclave series 2024</h4>
SCIEX offers the platform for the experts to take the stage and drive technological progress
Welcome to Pharma conclave series 2024 – where our customers’ expertise meets innovation, transforming your knowledge and empowering you to revolutionize your work processes. Join us in discovering techniques that will enhance your skills to accelerate your daily operations in the development of new drugs.
From the research of new markers to the drug development moving into non and GLP bioanalysis. Confidently plan your next lab expansion with the knowledge gained from industry leaders and pioneers.
<ul>
<li>May 16 Event 1: Pharma omics</li>
<li>September 17 Event 2: Impurities</li>
<li>October 23-24 Event 3: Pharma</li>
</ul>
10:00 a.m.–12:00 p.m. CET
<h3>Event 1: Pharma omics</h3>
Lost in translation? Translational research meets drug discovery and development – 3rd edition
<h5>Topics covered:</h5>
<ul>
<li>‘Pathophysiological subtypes of mild cognitive impairment due to Alzheimer’s disease identified by CSF proteomics’ by Bruno Manadas, at CNC
</li>
<li>‘Scalable and automated workflows for global plasma proteomics by standardized LC-MS’ by Dorte Bekker-Jensen, at Evosep
</li>
<li>‘Quantitative, standardized and reproducible metabolomics solutions in pharmaceutical R&amp;D’, by Stefan Ledinger and Markus Langsdorf, at Biocrates Life sciences
</li>
<li>Multi-omics analysis on mouse plasma in a pre clinical development of a drug in Alzheimer
</li>
</ul>
<blockquote>
Presenter: Bruno Manadas, PhD
</blockquote>
<blockquote>
Dr Manadas is a prominent Cell Biology researcher at CNC-UC since 2017. With expertise in biochemistry, psychology, and biotechnology, he&#39;s authored 130+ publications and led multiple projects. As head of CNC&#39;s Mass Spectrometry node and Associate Editor for Frontiers, he&#39;s contributed significantly to neuroscience and psychiatry. Bruno&#39;s research focuses on cell biology mechanisms, translational biomarker research, and MS-based approaches for studying protein oxidation. He played a pivotal role in establishing the hospital biobank and teaches at the Portuguese Catholic University.
</blockquote>
<blockquote>
Presenter: Dorte Bekker-Jensen
</blockquote>
<blockquote>
Dorte is heading the Applications Development team in Proteomics Research at Evosep. She has long-term experience within proteomics and has focused on technology development by developing simple and automated workflows for high-throughput applications.
</blockquote>
<blockquote>
Presenter: Stefan Ledinger
</blockquote>
<blockquote>
Stefan has 20 years of experience in the life sciences and diagnostic industries. For the last dozen years, he has been serving various functions within biocrates life sciences ag. Most recently, he has been responsible for the market development for metabolomics solutions in the pharmaceutical and biotech industries. He currently serves as Global Head of Sales. Stefan holds degrees in business administration and bioengineering, combining commercial know-how with a passion for science.
</blockquote>
<blockquote>
Presenter: Markus Langsdorf
</blockquote>
<blockquote>
Markus has 14 years of experience in life sciences industries. As he joined biocrates in 2010, he worked for 11 years as application support specialist for biocrates’ metabolomics profiling solutions. 3 years ago he changed his position withing the company to his new function as product manager. Markus studied chemistry and graduated with a PhD in Analytical Chemistry.
</blockquote>

Pharma conclave series 2024: Pharma omics

Discover techniques that will enhance your skills to accelerate your daily operations in the development of new drugs.

Drug testing can be performed in many biological matrices, such as urine, blood, saliva, sweat, hair and nail. Although urine and blood testing are the most common forms of drug testing, the use of keratinized matrices such as hair and nail is becoming extremely valuable in providing a broader picture of past drug consumption.
This is due to the incorporation of drugs consumed by the subject in the hair and nail keratin matrices, offering a much wider detection window than conventional biological matrices. In addition to the non-invasive nature of sample collection, hair and nail specimens are easy to transport and store, which minimizes the risk of sample alteration and degradation overtime. Despite its rise in popularity, drug detection in keratinized matrices remains challenging due to the low concentration of drugs embedded in the keratin matrix. As a result, hair and nail analysis requires sensitive instrumentation and extensive validation. 
This webinar will focus on the method development and validation of LC-MS/MS-based methods required to perform comprehensive and sensitive drug analysis in keratinized matrices. Case examples of the use of these methods will be demonstrated, highlighting the need for high-sensitivity mass spectrometry instrumentation for low-level detection of drugs and metabolites in hair and nail samples. The result presented will highlight the benefits of hair and nail analyses in forensic applications, enabling researchers to obtain significant retrospective information about past exposure and long-term use of drugs in the consumer’s population. 
Key learnings in this webinar will include:
<ul>
<li>Attendees will learn about the advantages of hair and nail testing to determine long-term drug exposure </li>
<li>Attendees will get an understanding of the importance of method development and validation of LC-MS/MS-based methods to perform comprehensive drug analysis in keratinized matrices</li>
<li>Attendees will discover the benefits of sensitive mass spectrometry instrumentation for the detection of low-level drugs and metabolites in keratinized matrices</li>
</ul>

Wiley: Hair and Nail Analysis in Forensic Applications

Hair and Nail Analysis in Forensic Applications

Development and validation of LC-MS/MS methods for drug analysis in keratinized matrices.

LCMS webinars focusing on LC/MS/MS - page 41