Ask any analytical chemist which hyphenated technique has become indispensable over the past decade, and the answer will almost certainly be the same: tandem chromatography–mass spectrometry. Whether it is <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis">LC–MS/MS</a> quantifying a novel biologic’s metabolic fate, or <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis">GC–MS/MS</a> chasing per- and polyfluoroalkyl substances (PFAS) down to sub-part-per-trillion levels in municipal water, the combination of chromatographic separation with the specificity of tandem mass detection has become the analytical workhorse of choice across virtually every regulated sector.Below we examine the key forces propelling this expansion, from biopharma pipeline dynamics and PFAS regulatory mandates to AI-driven automation and reshoring of pharmaceutical manufacturing—and explore what each trend means for laboratories managing sample preparation and analytical workflows.<h3>Biopharma: The Engine Room of LC–MS/MS Demand</h3><h4>GLP-1 Agonists and the Metabolomics Gold Rush</h4>The explosive commercial and scientific interest in GLP-1 receptor agonists—<a target="_blank" rel="noopener noreferrer" href="https://www.sepscience.com/advancing-glp-1-peptide-analysis-chromatography-challenges-and-innovations-12163">semaglutide and related peptide therapeutics </a>for diabetes and obesity—has dramatically expanded the analytical demands placed on tandem MS platforms. Discovery programs require precise quantification of peptide drug candidates and endogenous biomarkers across complex biological matrices. These workflows rely on high-sensitivity LC–MS/MS for pharmacokinetic profiling, receptor occupancy studies, and metabolomics characterization. Metabolomics is, in fact, projected to be the fastest-growing application segment in mass spectrometry, driven in part by its growing role in cancer screening, biomarker discovery, and metabolic disease research.I have been spending more time learning about GLP-1s because this is the hot topic that people want to discuss on the Concentrating on <a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL?si=n9GLz9zjSCOrVfXPxFPhng&amp;nd=1&amp;dlsi=52868e027aad4d22">Chromatography podcast</a>. With that said, I was astounded looking at CNBC.com during breakfast this morning to see that <a target="_blank" rel="noopener noreferrer" href="https://www.cnbc.com/2026/03/21/glp-1-diets-restaurants-protein-fiber-weight-loss-drugs.html">one in eight Americans are currently taking one of these drugs such as Ozempic</a>.<h4>Antibody–Drug Conjugates and Gene Therapy: Complexity at Every Step</h4>The ascent of antibody–drug conjugates (ADCs) and gene therapies has introduced a new level of analytical complexity. ADCs blend the target specificity of monoclonal antibodies with the cytotoxic potency of small-molecule payloads—and characterizing the linker chemistry, drug-to-antibody ratio (DAR), and conjugate stability demands high-resolution LC–MS/MS at every stage from discovery through QC release testing. Gene therapy vectors require equally rigorous characterization of impurities, aggregates, and empty/full capsid ratios.This translates into a direct need for low-evaporation, high-recovery concentration methods that preserve labile protein structures and maintain sample integrity prior to LC–MS/MS injection. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">Nitrogen evaporation workflows</a> that minimize oxidative stress and maintain sample temperature are increasingly critical in these pipelines.<h4>Precision Medicine and Multi-Omics Integration</h4>The shift toward precision medicine has accelerated adoption of high-throughput, multi-analyte MS platforms. Proteomics, metabolomics, and lipidomics workflows are converging on a single analytical platform—tandem MS—and generating datasets of a scale and complexity that were unimaginable ten years ago.Make no mistake: the triple quadrupole serves an irreplaceable role in quantitative targeted analysis. At the same time, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=134&amp;manufacturerItems=3">Orbitrap </a>and Q–TOF hybrid platforms are growing rapidly as researchers demand full-scan accurate mass for untargeted discovery.<h3>Environmental Mandates: PFAS Testing Drives Adoption</h3>Environmental testing is something that every person, chemist or not, can understand the importance of. Mounting mandates from the U.S. Environmental Protection Agency and European regulators regarding PFAS—the “forever chemicals” whose extraordinary environmental persistence is matched only by the analytical challenge of detecting them at trace levels—have created immediate, compliance-driven investment in LC–MS/MS infrastructure.<h4>The Regulatory Framework Chemists Need to Know</h4>The EPA has codified a suite of LC–MS/MS-based methods as the analytical standard for PFAS in water and solid matrices:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/epa-533-drying-down-pfas-samples-after-spe">EPA Method 533</a>: Weak anion exchange SPE combined with LC–MS/MS; covers 25 PFAS including short-chain compounds in drinking water.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/sample-concentration-epa-method-537-pfas-determination">EPA Method 537.1</a>: Reversed-phase SPE followed by LC–MS/MS; covers 18 PFAS including the GenX compound, with method detection limits in the low ng/L range.</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/en/solutions/environmental/water-testing/pfas-in-water/us-epa-method-8327?srsltid=AfmBOooCY1pcavj7B1PH-75d3HO4BGDglNlhmyTyrpDY3zvUksMO1dlg">EPA Method 8327</a>: LC–MS/MS for selected PFAS in prepared extracts of aqueous and solid matrices.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/preparing-diverse-sample-matrices-for-epa-method-1633">EPA Draft Method 1633</a>: The emerging benchmark for PFAS in non-aqueous matrices—aqueous, solid, biosolids, and tissue—quantifying up to 40 PFAS.</li></ul>All of these methods designate LC–MS/MS, specifically LC coupled to triple quadrupole or tandem-in-time platforms operating in negative electrospray ionization mode with multiple reaction monitoring (MRM), as the required or recommended analytical technique. PFAS analysis is, at its core, an LC–MS/MS problem.<h4>The Sample Preparation Bottleneck</h4>Chemists who have worked with PFAS methods understand the particular demands they place on sample preparation. The ubiquity of<a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/tackling-pfas-contamination-practical-insights-from-epa-537.1-method-development"> PFAS in laboratory materials</a>—PTFE tubing, some SPE sorbents, standard bench supplies—means that blank contamination control is as analytically demanding as the detection itself. EPA 537.1 requires that sample volumes of approximately 250 mL be extracted and reconstituted in as little as 1 mL, creating a 250:1 concentration factor that can expose even trace contamination in the sample processing chain. The installation of a delay column between the LC pump and autosampler to prevent instrument background interference is now standard practice.This is precisely where robust, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5385">inert nitrogen evaporation </a>systems earn their keep. PFAS workflows that use nitrogen-assisted evaporation to concentrate extracts prior to reconstitution benefit from materials that avoid PTFE and other fluoropolymers that can leach PFAS background—a consideration Organomation addresses in its concentration system designs.The analytical community is also moving beyond targeted PFAS analysis. High-resolution accurate mass (HRAM) spectrometry via Orbitrap technology, paired with non-targeted screening workflows, is gaining traction for the identification of the more than 5,000 currently catalogued PFAS compounds and the novel structures that continue to emerge. LC–MS/MS remains the quantitative gold standard; HRAM provides the discovery layer.<h3>Technological Trends Reshaping the LC–MS/MS Workflow</h3><h4>Artificial Intelligence and Automated Data Interpretation</h4>The datasets generated by modern tandem MS platforms—particularly in omics applications—are too large and too complex for conventional manual review. AI-driven spectral deconvolution, automated peak integration, and AI-assisted hypothesis generation are now embedded in the software suites accompanying leading instruments. <a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/about/newsroom/presrel/2022/17feb-gp22004.html?srsltid=AfmBOop3warRIaEojXM2YyMML6RXfMIDzE-Wm0h-SiKkkocBeeET2m7u">Agilent’s acquisition of Virtual Control’s ACIES </a>software specifically to automate LC–MS and GC–MS data analysis is emblematic of this direction.For laboratory managers, AI integration means smaller specialized teams can operate high-throughput multi-analyte workflows—but it also means that the front-end sample preparation must be even more reproducible, since algorithmic interpretation amplifies inconsistencies introduced upstream.<h4>Online SPE Coupling and “Chromatography Without Walls”</h4>A significant trend is the integration of solid-phase extraction directly into the HPLC flow path—so-called online SPE–HPLC coupling. Systems that combine extraction and chromatographic separation in a single analytical instrument reduce manual transfer steps, minimize the risk of sample loss or contamination, and dramatically increase throughput. For analytes at very low concentrations, online SPE provides the on-column concentration capability that traditional offline preparation achieves with manual evaporation steps.That said, offline sample preparation—including <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">nitrogen-assisted evaporation</a> under controlled conditions, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2407">liquid–liquid extraction</a>, and protein precipitation—retains significant advantages in flexibility, matrix handling, and the ability to process sample volumes that exceed online system capacity. The two approaches are increasingly complementary rather than competitive in well-designed analytical laboratories.<h4>High-Purity Solvents and Reference Materials</h4>As detection limits push further into the femtogram range and regulatory methods demand demonstrable blank performance, the quality of analytical consumables—mobile phase solvents, derivatization reagents, reference standards—has moved from background consideration to front-of-mind. Method validation in regulated environments now routinely requires vendor certification of solvent purity at a level that directly interfaces with instrument performance.<h3>The Ever-Present Need for Sample Evaporation</h3>As LC–MS/MS methods push detection limits lower, the analytical burden shifts increasingly upstream. The quality of the concentration, cleanup, and reconstitution steps preceding injection determines whether the instrument’s theoretical sensitivity is achievable in practice.Several specific developments are particularly relevant:<ul><li>ADC and gene therapy characterization workflows require gentle, low-temperature evaporation that preserves protein structure and prevents aggregation prior to LC–MS/MS. Nitrogen evaporation under controlled conditions is the preferred approach.</li><li>PFAS trace analysis demands evaporation systems constructed from PFAS-inert materials, with rigorous attention to avoiding fluoropolymer surfaces throughout the sample path.</li><li>High-throughput pharmaceutical QC driven by reshoring demands parallel processing capability—multi-position evaporators that maintain consistent flow and temperature across all positions simultaneously.</li><li>Multi-omics and metabolomics workflows generate large sample batches that benefit from automated, walk-away evaporation with programmable endpoints to ensure reproducible final volumes across a cohort.</li></ul>Tandem chromatography–mass spectrometry is not merely growing; it is becoming structurally embedded in the analytical workflows of every major regulated industry. The convergence of biopharma complexity, PFAS regulatory mandates, AI-enabled data analysis, and pharmaceutical reshoring is creating a multi-year demand cycle that will require laboratories to invest not only in instruments, but in the entire sample preparation ecosystem that makes those instruments perform.For analytical chemists, the practical takeaway is this: as mass spectrometry sensitivity continues to advance, the sample preparation step becomes the binding constraint on method performance. Whether you are developing a new PFAS method to meet EPA 1633, characterizing an ADC’s drug-to-antibody ratio, or running a 500-sample metabolomics cohort overnight, the reliability and cleanliness of your concentration and cleanup workflow determines the quality of every data point that follows.&nbsp;

The Continued Rise of Tandem Chromatography–Mass Spectrometry

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

Article

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Application

Academy

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Technology Networks

Bruker

Thermo Fisher Scientific

Wiley

SCIEX

Evosep Biosystems

Waters Corporation

ADCs represent complex biologics combing a targeting antibody molecule with a cytotoxic warhead via a linker system. The design of the linker systems is crucial to ensure the warhead is only released at the target site, whilst enabling conjugation to an active site on the protein.
Mass spectrometry can be utilized in various modalities to monitor loss of payloads when incubated in plasma providing a rapid measurement of relative stability.
This webinar will detail how mass spectrometry is used in ADC development and how the X500B QTOF system represents a versatile platform for measurement of intact, reduced, and digested proteins.
Attend this webinar to:
<ul>
<li>Understand how LC-MS can be used to study the stability of an ADC in plasma</li>
<li>Find out how LC-MS can be used to calculate the antibody drug ratio of an intact ADC</li>
<li>Discover how LC-MS has been used to study the payload release of an ADC</li>
</ul>
<blockquote>
Presenter: Chris Nortcliffe (Mass Spectrometry Manager | Sterling Pharma Solutions | UK)
</blockquote>
<blockquote>
Dr Christopher Nortcliffe is the mass spectrometry manager in the UK at Sterling Pharma Solutions, a CDMO pharmaceutical partner company. He has a background in protein and DNA analysis, obtaining his PhD from the University of Manchester on studying noncovalent interactions by mass spectrometry in 2016. After Manchester, Christopher worked for SCIEX as a specialist in LC-MS analysis of biologics before moving to Sterling Pharma Solutions, where he manages the LC-MS service and is an expert in the LC-MS analysis of biotherapeutics and ADCs.
</blockquote>

SCIEX: Use of mass spectrometry to assess plasma stability of Antibody Drug Conjugates

Use of mass spectrometry to assess plasma stability of Antibody Drug Conjugates

How mass spectrometry is used in ADC development and how the X500B QTOF system represents a versatile platform.

Use of mass spectrometry to assess plasma stability of ADCs

The field of synthetic biology revolves around the design-build-test-learn (DBTL) cycle, where biological components are adjusted in multiple iterations. Automation allows synthetic biologists to spend less time pipetting liquids and devote more time understanding the science and finding creative solutions to problems.
By combining automation for sample preparation with rapid separation methods we provide a phenotyping workflow suited to the needs of the synthetic biology community.
Attend this webinar to discover:
<ul>
<li>Automation solutions for synthetic biology applications</li>
<li>Rapid separation method workflows</li>
<li>How to improve confidence in annotation using ion mobility</li>
</ul>
<blockquote>
Presenter: Dr. Tessa Moses (Metabolomics Specialist and Facility Manager of EdinOmics, Center for Engineering Biology, The University of Edinburgh)
</blockquote>

Technology Networks: An Automated Workflow for Untargeted Metabolomics of Complex Biological Samples

An Automated Workflow for Untargeted Metabolomics of Complex Biological Samples

By combining automation for sample preparation with rapid separation methods we provide a phenotyping workflow for the synthetic biology community.

An Automated Workflow for Untargeted Metabolomics

Unlock new perspectives with our accurate mass 101 webinar series.
Accelerate your workflows and expand your knowledge around accurate mass with these informative webinars.
In this webinar series, SCIEX applications scientists will explain the benefits of using accurate mass, sample analysis, instrument operation and data processing in real-time.
Get the inside scoop and register today!
<h3>Accurate mass 101: non-targeted analysis</h3>
Join us for this webinar to see how to discover the unknown using non-targeted analysis. We will showcase workflows and strategies to drive success with suspect screening and non-target identification. Discover the ability to identify peaks of interest and confidently identify unknown compounds.

SCIEX: Accurate mass 101: non-targeted analysis

Accurate mass 101: non-targeted analysis

SCIEX applications scientists will explain the benefits of using accurate mass, sample analysis, instrument operation and data processing in real-time.

As an analytical scientist, you need robust, flexible, and easy solutions to help you characterize and understand your oligonucleotides faster.
Have a closer look at the Waters’ solution:
Exceptional LC-MS performance to maximize sample analysis and comprehensive informatics capabilities, specifically designed for biopharmaceutical analytical laboratories that need to be able to make more confident and more efficient analytical decisions.
Join our 30 Min live session, directly from the Waters Wilmslow Demo Lab:
<ul>
<li>Find out how Waters solutions can help you to know more about your oligonucleotides – quicker and easier than ever!
</li>
<li>Learn about the benefits of a flexible system and gain insight into this exciting solution and how it can help your lab overcome analytical challenges.
</li>
<li>Discover the Waters solution for extended Oligonucleotide analyses, including a preview of the INTACT Mass app and the CONFIRM Sequence app in waters_connect
</li>
</ul>
Waters MS Systems - including ACQUITY Premier LC-System, MaxPeak Premier Columns, and waters_connect Software - are a workflow-based solution: It is easy to implement and operate and will improve decision-making and time to results in your laboratory.
Join Dr. Jo-Anne Riley for this live demo. She will show you how easy it is to set up and operate the Xevo G3 QTof system and introduce you to our INTACT Mass and CONFIRM Sequence workflows from samples to results.

Waters Corporation: Oligonucleotide Analyses using LC-MS: A Live Demo

Oligonucleotide Analyses using LC-MS: A Live Demo

Find out how Waters solutions can help you to know more about your oligonucleotides – quicker and easier than ever!

Unleash the power of DIA and enable the exploration of complex biological systems with unprecedented depth and precision for large-scale quantitative protein analysis combined with short methods on the Evosep One.
<h3>Global proteomics with Evosep and DIA-PASEF</h3>
Recent progress in liquid chromatography and mass spectrometry, as well as sample preparation and search algorithms for spectra deconvolution and identification, plays an important role in bringing proteomics to the forefront of drug discovery and development. Nanoflow liquid chromatography coupled to tandem mass spectrometry is one of the most essential elements in a proteomics workflow. The four dimensional ion detection achieved with the latest generation of Bruker’s trapped ion mobility spectrometers led to superior speed and sensitivity. Evosep One liquid chromatography platform offers speed and robustness while maintaining, even exceeding previously achieved performance level with high-performing nano LC systems. We share here some results only possible with this combination of LC-MS and dia-PASEF.
<blockquote>
Presenter: Anh Reynolds (Scientist, Bristol Myers Squibb)
</blockquote>
<h3>Extending sensitivity limits with Evosep and Slice-PASEF</h3>
Sensitivity improvements in LC-MS proteomics have recently led to exciting new applications, such as comprehensive proteomic profiling of single cells or 2-dimensional proteomics of tissue slices. In this talk, I will describe Slice-PASEF, a new acquisition technology for timsTOF instruments, which, in combination with the DIA-NN data analysis software, provides a fold-change signal boost. When paired with the Evosep system, Slice-PASEF allows to achieve high sensitivity in robust high-throughput workflows, paving a way for accessible sensitive proteomics on a large scale.
<blockquote>
Presenter: Vladim Demichev (Group Leader, Quantitative ProteomicS, Charité – Universitätsmedizin Berlin)
</blockquote>

Pixabay/sethink: Revolutionizing proteomics with Evosep-DIA

Revolutionizing proteomics with Evosep-DIA

Global proteomics with Evosep and DIA-PASEF & Extending sensitivity limits with Evosep and Slice-PASEF.

Single cell proteomics is growing as an analytical tool, delivering in-depth information of molecular processes occurring at the cellular level. The release of the timsTOF SCP in June 2021 opened the gate to label-free analysis of single cells.
This approach removes the needs of labelling and their associated costs and bias. Removing this step is not sufficient to handle all the challenges associated with the extremely diluted sample preparation, handling and processing.
In this live from the lab, we will present and demonstrate the use of a complete SCP ecosystem allowing to address the sensitivity and robustness issues at all stages of the analytical process, starting from single cell sorting and digestion down to the data analysis, considering sample transfer, separation, dia-PASEF® acquisition and real-time data processing.

Bruker: Single cell proteomics, from sample preparation to results processing

Single cell proteomics, from sample preparation to results processing

We will demonstrate a complete SCP ecosystem and address different challenges associated with diluted sample preparation, handling, and processing.

Single cell proteomics, from sample preparation to results

Cell line development scientists and upstream bioprocess engineers aim at optimizing biotherapeutics production processes to maximize drug product quality, yield, and manufacturing efficiency. Currently, information on critical process and product attributes required for informed decision making is often insufficient and not readily available. This has a negative impact on the process robustness, throughput, and outcome.
Direct measurement of cell culture media and product quality attributes (PQAs) has recently been offered thanks to the Waters BioAccord™ LC-MS system with dedicated workflows for process analytics of biopharmaceuticals. The BioAccord™ LC-MS can be directly coupled to multi parallel bioreactor systems, like the Sartorius Ambr® 15 or 250 HT platforms, for accelerated process development. 
With this integrated solution, process engineers have direct access to the information they need to be in control: drug substance quality and yield can be maximized, process and product related impurities minimized, and more robust processes can be developed, thereby de-risking batch failure. This ultimately results in accelerated drug product development and manufacturing release from months or weeks to days, saving time and resources but more importantly enable faster delivery of safer treatments to patients. 
Please join us as Magnus Wetterhall will present how Waters BioAccord™ LC-MS system offer enhanced capabilities for direct measurements of critical process and product quality attributes in cell line and process development and thereby enabling you to make more informed decisions with your own high-quality data. 
<blockquote>
Presenter: Magnus Wetterhall, Ph.D. (Global Marketing Manager – Bioprocessing, Waters Corporation)
</blockquote>
<blockquote>
Magnus Wetterhall has more than 25 years’ experience in upstream &amp; downstream bioprocessing, advanced mass spectrometry, biomarker discovery, protein characterization, working at start-ups, large biotech companies as well as academics. Magnus earned his M.Sc. and Ph.D. degree in analytical chemistry at Uppsala University, Sweden. He has published over 40 scientific papers and 50 posters in the areas of mass spectrometry, biomolecule characterization and bioseparations, and has been an invited presenter at national and international conferences on these topics. He is currently the Global Marketing Manager at Waters Corporation – Bioprocessing, where he is dedicated to deliver analytical solutions to advance upstream and downstream bioprocess.
</blockquote>
<blockquote>
Presenter: Terence Teo (BioPharma/BMR Market Development Manager, APAC, Waters Corporation)
</blockquote>
<blockquote>
Terence Teo has more than 15 years of experience in bioprocessing, specializing in downstream processing R&amp;D during the early phase of his career before moving to business development roles in GE Life Sciences (now Cytiva), Pall Biotech and most recently, Waters Corporation. Terence is motivated in supporting regional customers in APAC with technologies enabling continuous bioprocessing. He is currently working actively in Waters to drive the use of LC-MS analytical solutions to support near real-time CQA measurement in bioprocess applications, as well as use of LC-MS in multi-attribute method (MAM) and emerging drug modalities such as mRNA and viral vectors. Terence earned his M.Eng degree in Chemical and Biomolecular Engineering at National University of Singapore (NUS).
</blockquote>

Waters Corporation: Accelerating Upstream Bioprocess Development Through Data Driven Decisions

Accelerating Upstream Bioprocess Development Through Data Driven Decisions

We will present how Waters BioAccord™ LC-MS system offer enhanced capabilities for direct measurements of critical process and product quality attributes.

Accelerating Upstream Bioprocess Development

This webinar will focus on untargeted LC/MS Omics solutions that will provide the biological insights needed to produce meaningful results. An emphasis will be spent on an End-to-End HILIC metabolomics workflow for our LC/Q-TOF platform.
This includes automate sample preparation for cells and plasma, a Bio LC that is fit for purpose for metal sensitive analysis, robust and transferable HILIC chromatography, the 6546 LC/Q-TOF data independent analysis of samples, with an ID workflow that includes a new HILIC PCDL with 500 core metabolism analytes with spectra and retention time annotation.
For unknowns not found in Agilent PCDLs, SIRIUS/CSI:FingerID which uses AI predict structure from spectra and conducts online identification search will be demonstrated. A complementary targeted multi-omics workflow will be briefly mentioned before sharing the new Ion Mobility and ExD technologies for high end LC/MS Omics investigations.
<blockquote>
Presenter: Karen E. Yannell (Omics Workflow Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Karen E. Yannell is an Omics Workflow Specialist at Agilent Technologies. She is an expert in small molecule high throughput analysis by triple quadrupole (TQ) and quadrupole time-of-flight (Q-TOF) mass spectrometry for applied markets. Her recent work has focused on developing targeted metabolomics methods and routine Q-TOF workflows for both expert and non-expert users alike. Previously, Karen worked on forensic, metabolomics, and biomarker discovery applications with ambient ionization sources. She received her PhD in Analytical Chemistry from Purdue University where studied under the advisement of Prof. R. Graham Cooks. 
</blockquote>
<blockquote>
Presenter: Dr. Martin Graf-Utzmann (Editor, Wiley Analytical Science, Moderator)
</blockquote>
<blockquote>
Dr. Martin Graf-Utzmann has a Ph.D. in Theoretical Chemistry and is an editor for Wiley Analytical Science. His interests are mainly Laboratory Automation, Digitalization, and Lab Equipment.
</blockquote>

Wiley Analytical Science: Jump Start Your LC/Q-TOF Omics Investigation with Reliable Workflows

Jump Start Your LC/Q-TOF Omics Investigation with Reliable Workflows

This webinar will focus on untargeted LC/MS Omics solutions that will provide the biological insights needed to produce meaningful results.

Your LC/Q-TOF Omics Investigation with Reliable Workflows

This year we invite you to ignite your mind to Be Curious about what Ion chromatography (IC) can do with its versatility in many detections across the IC application spectrum to improve your workflows and solve challenges.
During this three day virtual event, learn from a panel of experts how the latest IC applications are helping colleagues achieve breakthroughs, and walk away with valuable information on how advancements in IC can increase laboratory analytical capabilities. 
Although each day is convenient for a specific region, you can attend any session on any day. All sessions and workshops will be available for on-demand viewing after the event concludes.
<h3>Day 1 Agenda | April 18 | North and South America</h3>
Discover IC column development, and where it’s headed with mass spectrometry, as they can be an integral detection for carbohydrate profiling and environmental metabolites characterization. Learn how standalone IC can solve industrial samples and degradation products with comprehensive IC information, all at once.
<h5>11:30 EDT: Opening Plenary: The Past, Present, and Future of IC Column Development</h5>
<ul>
<li>Alexandra Zatirakha (Thermo Fisher Scientific, IC/SP R&amp;D, Sunnyvale)</li>
</ul>
<h5>12:30 EDT: Characterizing Carbohydrate Degradation Products from Bacteroides Thetaiotaomicron to Understand Bacterial Dextran Utilization in the Gut</h5>
<ul>
<li>Neil Rumachik (Thermo Fisher Scientific, IC/SP R&amp;D, Sunnyvale)</li>
<li>Fiona Cuskin (Newcastle University, UK)</li>
</ul>
<h5>1:30 PM EDT: Toward Comprehensive Chemical Characterization of Metabolomic Using Orbitrap Fusion Tribrid Mass Spectrometer Coupled with Ion and Liquid Chromatography Techniques: Dissolved Organic Matter as an Example</h5>
<ul>
<li>Hussain Abdulla (Department of Physical and Environmental Sciences, Texas A&amp;M University-Corpus Christi)</li>
</ul>
<h5>2:30 PM EDT: The Use of Ion Chromatography in the Monitoring of Degradation Products from the Electrochemical Treatment Of Organic Compounds</h5>
<ul>
<li>Emily Tossi (Federal University of Rio Grande do Norte)</li>
</ul>
<h5>3:30 PM EDT: Ion Chromatography as a Tool for Industrial Problem Solving</h5>
<ul>
<li>Isaac Negrete and Stephen Fosdick (Dow)</li>
</ul>
<h5>4:30 PM EDT: Workshop: Everything, Everywhere, All at Once IC!</h5>
<ul>
<li>Kelly Femrite (IC/SP Product Support, Sunnyvale)</li>
<li>Carl Fisher (IC/SP Product Marketing, Sunnyvale)</li>
<li>Criss Corchard-Keeler (Service Marketing Manager, CMD/MOL Thermo Fisher Scientific)</li>
</ul>

Thermo Fisher Scientific: IC Symposium 2023

IC Symposium 2023 - Day 1

Join us for the 2023 Global IC Symposium! North and South America

This year we invite you to ignite your mind to Be Curious about what Ion chromatography (IC) can do with its versatility in many detections across the IC application spectrum to improve your workflows and solve challenges.
During this three day virtual event, learn from a panel of experts how the latest IC applications are helping colleagues achieve breakthroughs, and walk away with valuable information on how advancements in IC can increase laboratory analytical capabilities. 
Although each day is convenient for a specific region, you can attend any session on any day. All sessions and workshops will be available for on-demand viewing after the event concludes.
<h3>Day 3 Agenda | April 20 | Asia Pacific</h3>
Expand the world of electrochemistry applications and IC hyphenation with high-resolution mass spectrometry and understand how Inductively Coupled Plasma Spectrometry can speciate isotopes while simplifying Pharmacopeia QC analysis and keeping your IC in top-notch condition.
<h5>11:00 AM SGT: Opening Plenary: Electrochemical Detection and Its Application in Ion Chromatography</h5>
<ul>
<li>Yan Liu (Thermo Fisher Scientific, IC/SP R&amp;D, Sunnyvale)</li>
</ul>
<h5>12:00 PM SGT: Application of Anion Chromatography Coupled High-Resolution Mass Spectrometry to Increase Cellular Metabolite Coverage</h5>
<ul>
<li>Vinzenz Hofferek (McConville Lab, Department of Biochemistry and Pharmacology)</li>
</ul>
<h5>1:00 PM SGT: Analysis of Anhydrosugars and Xylooligosaccharides by Ion Chromatography with Electrospray Lithium Cationisation-Triple Quadrupole Tandem Mass Spectrometry</h5>
<ul>
<li>Dr. Estrella Sanz Rodriguez (Natural Products Analysis, Australian Centre for Research on Separation Science (ACROSS), University of Tasmania)</li>
</ul>
<h5>2:00 PM SGT: You Gotta Keep ‘Em Separated: Novel IC Applications for Subsequent Isotopic Analyses by Rapid and/or Hyphenated Techniques</h5>
<ul>
<li>Dr. Brandon Mahan (College of Science and Engineering, James Cook University)</li>
<li>Grace Manestar (IsoTropics PhD Candidate)</li>
</ul>
<h5>3:00 PM SGT: Use of Ion Chromatography in Pharmaceutical Quality Control</h5>
<ul>
<li>Catherine Frankis and Stella Planou (Reading Scientific Services Ltd., Reading Science Centre, Whiteknights Campus)</li>
</ul>
<h5>4:00 PM SGT: Tips on Keeping Your Ion Chromatography in Top-Notch Condition</h5>
<ul>
<li>Ryan Ong (Thermo Fisher Scientific, IC/SP Application Sales Support, Singapore)</li>
</ul>

LCMS webinars focusing on LC/HRMS - page 42

IC Symposium 2023 - Day 3