Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

News from LabRulezLCMS Library - Week 20, 2026

🎤Lindsay Repka&nbsp;Lindsay Repka is a chemistry professor at Middlebury College whose research explores photoredox chemistry, catalyst stability, and visible-light-driven transformations. Her lab emphasizes both mechanistic insight and hands-on student training in advanced analytical instrumentation.In this episode of Concentrating on Chromatography, we sit down with Lindsay Repka to discuss how LC-MS and GC-MS transformed her lab’s approach to photoredox chemistry.What began as a project to develop a visible-light photocrosslinking handle unexpectedly led to a major discovery: the solvent (DMF) was reacting with the photocatalyst itself. Using high-resolution LC-MS, Lindsay’s team observed multiple solvent adducts forming — sometimes with complete catalyst consumption. That discovery reshaped their research direction.This episode is a rare deep dive into both LC-MS and GC-MS within the same research project, showing how chromatography-driven insight can turn unexpected degradation into productive new reactivity.If you work in:<ul><li>Photoredox chemistry</li><li>Reaction optimization</li><li>Mass spectrometry method development</li><li>Catalyst screening</li><li>Academic synthetic chemistry</li></ul>…this conversation will resonate.<h4>Key Topics Covered</h4><ul><li>Photocatalyst stability in DMF, DCE, and MeCN</li><li>Demethylation under mild visible-light conditions</li><li>High-resolution Q-TOF LC-MS quantitation</li><li>Internal standard methodology in GC-MS</li><li>Signal-to-noise improvement using extracted ion chromatograms</li><li>Reaction reproducibility and quality control strategy</li></ul><h3>Video Transcription</h3>In this interview, Lindsay Repka discusses the role of photoredox chemistry in method development, photocrosslinking, solvent activation, catalyst stability, and the use of LC-MS and GC-MS to understand reaction pathways.<h4>What is photoredox chemistry?</h4>Photoredox chemistry uses light to activate a photocatalyst, which can either donate or accept an electron from a substrate. This generates radical species that can undergo new types of reactivity.Repka explains that one of the key advantages of photoredox chemistry is its ability to operate under mild conditions, often using visible light. This makes it attractive for sensitive molecules and biological systems, including potential applications in drug release, live-cell chemistry, and drug development.<h4>Photocrosslinking and drug-target interactions</h4>Repka’s group initially aimed to develop visible-light photocrosslinking methods to study drug interactions with biological targets. Photocrosslinking can covalently link a drug to its target, making it easier to identify the biological target of a compound.Traditional photocrosslinking often uses UV light, but visible light offers milder and more biologically compatible conditions.<h4>From photocrosslinking to solvent activation</h4>The project shifted direction when the desired photocrosslinking reactions gave very low yields. Instead of reacting with amino acids as intended, the radical intermediate mainly reacted with itself.The team explored different solvents to stabilize the radical, but found that some solvents, such as DMF, were not inert. Instead, the solvent reacted with the photocatalyst or radical species, revealing unexpected solvent activation chemistry.<h4>Why LC-MS was critical</h4>LC-MS became essential because the reaction mixtures were too complex and low-yielding for NMR or GC-MS. High-resolution LC-MS allowed the team to detect minor products, identify photocatalyst modifications, and observe solvent addition products with high confidence.Repka emphasizes that LC-MS was both a qualitative and quantitative tool. It was used quantitatively to measure how much photocatalyst remained after light exposure, and qualitatively to identify degradation products and new reactivity pathways.<h4>Photocatalyst stability workflow</h4>The team developed a systematic workflow to evaluate photocatalyst stability under light exposure. Because catalyst degradation was time-sensitive, reproducibility required strict control of experimental conditions.Key steps included using a glove box, commercial deoxygenated solvents, accurate weighing on a microbalance, controlled light exposure in a photoreactor, and temperature control with a chiller. These precautions helped reduce day-to-day variability and enabled reproducible measurements of catalyst remaining.<h4>Quantifying catalyst remaining by LC-MS</h4>Calibration curves were prepared using pure photocatalyst solutions and LC-MS ion areas. The team worked in the nanomolar range to maintain linearity.Instead of relying only on total ion chromatograms, they used extracted ion chromatograms (EICs) to improve signal-to-noise for the photocatalyst signal. Baseline subtraction was also applied to reduce noise and improve quantitation at low concentrations.Calibration curves and quality control samples were prepared and measured on the same day as the reaction samples, because LC-MS signal intensity can vary significantly from day to day.<h4>Why GC-MS was used later</h4>For demethylation studies, the group moved to GC-MS because the substrates and products were small and volatile enough for gas chromatography. GC-MS was better suited for rapidly screening many reaction conditions, including different photocatalysts, solvents, and oxidants.Instead of generating full calibration curves for every experiment, the team used an internal standard to streamline analysis and determine percent conversion based on relative response factors.<h4>Practical lessons from the project</h4>Repka highlights the importance of careful sample preparation, especially when working with very small quantities of photocatalysts. A high-quality microbalance, stable granite platform, antistatic tools, and aluminum weigh boats improved weighing accuracy and reproducibility.She also argues that mass spectrometry should be a standard part of photoredox method development because it can reveal minor products, support reaction optimization, and provide insights that may be difficult or impossible to obtain by NMR alone.<h4>Final perspective</h4>Beyond the chemistry, Repka emphasizes the importance of student training and mentoring. For her, research is not only about discovering new reactivity but also about giving students practical experience, helping them develop independence, and preparing them for future scientific careers.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Using LC-MS & GC-MS to Decode Photoredox Catalyst Stability

Article

Application

Video

Scientists

Interview

Science and research

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Agilent Technologies

Phenomenex

Thermo Fisher Scientific

C&EN

Shimadzu Corporation

SCIEX

Evosep Biosystems

Waters Corporation

Oligonucleotides (oligos) have become fast-growing modalities in recent years. Along with the development of these candidates has come the increased need for robust analytical methods and easy-to-use data analysis workflows to characterize them. Critical quality attributes of oligos include their mass, purity, and sequence – each of which can be difficult, time consuming, and tedious. As such, software that supports these efforts can be of great value.
In this work, we present novel, automated, and integrated software to support these workflows using LC HRAM MS instruments. Purity and sequencing data are presented for a multitude of oligos including antisense, aptamer, and long synthetic samples.
Key Learning Objectives:
<ul>
<li>Techniques for analyzing the critical quality attributes (CQAs) of oligos — including their mass, purity, and sequence</li>
<li>How novel, automated, and integrated software can facilitate the analysis of oligo CQAs using LC–HRAM MS instruments.</li>
<li>Purity and sequencing data for a multitude of oligos including antisense, aptamer, and long synthetic samples</li>
</ul>
Who Should Attend:
<ul>
<li>Researchers analyzing the critical quality attributes (CQAs) of oligos — including their mass, purity, and sequence</li>
<li>Chromatographers</li>
<li>Researchers interested in using LC–HRAM MS instruments for determination of oligo mass, purity and sequence</li>
</ul>
<blockquote>
Presenter: Peter Rye (Application Engineer Pre Sales, Agilent Technologies)
</blockquote>
<blockquote>
Presenter: Melissa O&#39;Meara (Forensic Science Consultant, C&amp;EN Media Group)
</blockquote>

C&EN: Recent Advances in Oligo Purity and Sequence Determination by LCMS

Recent Advances in Oligo Purity and Sequence Determination by LCMS

In this work, we present novel, automated, and integrated software to support oligos workflows using LC HRAM MS instruments.

Advances in Oligo Purity and Sequence Determination by LCMS

<ul>
<li>Receive a demonstration of how non-denaturing SEC-Native MS can be leveraged to streamline mAb characterization, including methods for the digestion of mAbs
</li>
<li>Listen in to a column selection discussion, which includes pore volume and column dimensions to ensure monomer and aggregate resolution, and optimize mobile phases for complex characterizations
</li>
<li>Learn about SEC combined with nHRMS, and how to select a SEC column
</li>
</ul>
Who Should Attend:
Any scientists wanting to learn how to combine their size exclusion chromatography and High-resolution Mass Spec for improvements in their mAb characertizations. 
<blockquote>
Presenter: Lorne Nelson (Senior Scientist - Field Applications, Phenomenex)
</blockquote>

Phenomenex: SEC-nHRMS for the Characterization of mAbs

SEC-nHRMS for the Characterization of mAbs

Receive a demonstration of how non-denaturing SEC-Native MS can be leveraged to streamline mAb characterization, including methods for the digestion of mAbs

Sepsis is a growing global challenge due to factors such as high mortality rate and the increasing prevalence of antimicrobial resistance.
<h4>A single and rapid Zeno SWATH DIA proteomic analysis for concomitant identification and antibiotic resistance profiling of micro-organisms from positive blood cultures</h4>
<ul>
<li>Presenter: Iulia MACAVEI, PhD (student in Analytical Chemistry, Biological Analysis and Mass Spectrometry, University of lyon)</li>
</ul>
Today, approximately 1.3 million people die each year worldwide from bacterial antimicrobial resistance (AMR). AMR is of great concern in the context of bloodstream infections (BSIs) when it evolves into sepsis. If MALDI-TOF has markedly shortened the delay of the pathogen identification step, AMR evaluation is still based on growth tests in the presence of antibiotics. With the aim of streamlining this turnaround time to less than 90min from a positive blood culture, the LC/Zeno SWATH DIA sensitivity and specificity performances were evaluated to concomitantly provide the identity and antibiotic resistance profile of etiological agents related to BSIs based on peptide surrogates.
<h4>Plasma proteomic landscape and patient heterogeneity in sepsis revealed by high-throughput mass spectrometry</h4>
<ul>
<li>Presenter: Dr. Yuxin Mi (Researcher at Wellcome Centre for Human Genetics, University of Oxford)</li>
</ul>
Incomplete knowledge of the sepsis plasma proteome has limited our understanding of pathophysiology in the heterogeneous syndrome of sepsis. Here we apply high-throughput liquid chromatography – tandem mass spectrometry to delineate the plasma proteome for sepsis and comparator groups, involving 2622 samples in a single batch. We show how this scale of data can establish shared and specific proteins, pathways and co-expression modules in sepsis, and be integrated with paired leukocyte transcriptomic data. This work reveals novel subphenotypes informative for the response state and differential outcome, highlighting opportunities for a systems-based precision medicine approach to sepsis.

SEPSIS

In this webinar we will uncover how our users combine technology with the Evosep One to further research into sepsis and its biomarkers.

Evosep SEPSIS

The ZenoTOF 7600 system was ranked the most innovative technology by The Analytical Scientist for its ability to help scientists address analytical challenges across many markets. Zeno trap coupled with electron activated dissociation (EAD) fragmentation technology unlocks sensitivity gains allowing you to uncover new information for certainty in your results to make better-informed decisions, faster.
Highlights of this technology:
<ul>
<li>Drive new limits of quantification achievable with accurate mass</li>
<li>Detect up to 20x more ions in every experiment</li>
<li>Deliver high depth of coverage on low abundance species, quickly and robustly with Zeno SWATH data-independent acquisition (DIA)</li>
</ul>
<blockquote>
Presenter: Bhavya Shukla (Sales Development Manager, SCIEX)
</blockquote>
<blockquote>
Bhavya Shukla, Sales Development Manager at SCIEX has more than 8 years of experience in the analytical industry. He pursued his post-graduation in chemical engineering from the Institute of Chemical Technology Mumbai. His major role is to support LCMS users by providing them optimal solutions through a keen understanding of their needs.
</blockquote>

SCIEX: Leveraging the power of Zeno trap and EAD

Leveraging the power of Zeno trap and EAD

Zeno trap coupled with electron activated dissociation (EAD) fragmentation technology unlocks sensitivity gains allowing you to uncover new information

Therapeutic monoclonal antibody (mAb) biosimilars are booming in Asia and across the world, as well as faces significant challenges in quality control.
Peptide mapping plays a critical role in structure characterization of antibody drugs. This webinar will introduce 4 Application News for structure characterization of bevacizumab biosimilar using LCMS-9030 Q-TOF mass spectrometry, including peptide mapping, C-terminal sequencing, disulfide bond analysis, and N-glycan analysis.
Learning Objectives:
<ul>
<li>Understand the critical quality attributes of biosimilar mAb 
</li>
<li>Explore how LCMS-QTOF can be used to characterize Bevacizumab biosimilar (Peptide mapping, terminal sequencing, disulfide bonds and N-glycan analysis)
</li>
</ul>
<blockquote>
Presenter: Lu Yonghai (Ph.D.) (Senior Application Chemist, Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Lu earned his PhD degree in Biology from the Chinese University of Hong Kong. In September 2018, Lu joined Shimadzu (Asia Pacific) as a Senior Application Chemist in Life Science and Advanced MS. Prior to working at Shimadzu, Lu was a Senior Scientist at the National University of Singapore in a Multi-Omics research lab specializing in LC-MS and GC-MS analysis.
</blockquote>

Shimadzu: LCMS-9030 Q-TOF for peptide mapping and structural characterization of mAb biosimilars

LCMS-9030 Q-TOF for peptide mapping and structural characterization of mAb biosimilars

4 Application News for structure characterization of bevacizumab biosimilar using LCMS-9030 Q-TOF mass spectrometry, including peptide mapping.

LCMS-9030 Q-TOF for peptide mapping and mAb biosimilars

The increasing complexity of the food supply chain, the exponential growth of chemical hazards and their regulations have brought several challenges to the field of food safety.
The need for productive gains and increased confidence in reporting non-compliant samples are particularly noteworthy challenges for laboratories that are routinely relied upon for maintaining a safe food supply chain. To meet these demands, mass spectrometric techniques such as tandem mass spectrometry and quadruple-time-of-flight mass spectrometry have emerged as the detectors of choice for the analysis of organic chemical hazards in foods and beverages.
One of the more recent significant developments is Shimadzu’s ultra fast family of mass spectrometric detectors that provide opportunities to increase both productivity and reporting confidence compared with more conventional mass spectrometric detectors. The importance of these state-of-the-art instruments to the analysis of chemical hazards in food will be discussed in the context of the regulatory requirements for the analysis of contaminants in foods and a general overview of chemical hazards in foods.
By viewing this webinar you will:
<ul>
<li>Understand the core challenges for food testing
</li>
<li>Recognize the key elements that can accelerate and streamline your workflow and increase your data confidence
</li>
<li>Learn and apply in various food safety analysis (e.g. multi-residual pesticides, veterinary drugs and mycotoxins).
</li>
</ul>
Who should view it?
<ul>
<li>Scientists and lab personnel conducting food analysis
</li>
<li>Lab managers in testing lab, regulatory agency and the food industry
</li>
<li>Everyone who is keen to increase their lab productivity and data confidence for food and beverage analysis.
</li>
</ul>
<blockquote>
Presdenter: Dr Peter Varelis (Applications Manager, Shimadzu Scientific Instruments (Oceania) )
</blockquote>
<blockquote>
Dr Peter Varelis has more than 20 years of research experience in both government and industry. He was also on an Elsevier editorial board (Food Chemistry and Analysis). His current research interest is the application of mass spectrometry to investigate organic compounds that have implications for human health and nutrition.
</blockquote>

Shimadzu: The Importance of Ultra Fast Mass Spectrometry for the Analysis of Chemical Hazards in Food & Beverages

The Importance of Ultra Fast Mass Spectrometry for the Analysis of Chemical Hazards in Food & Beverages

Understand the core challenges for food testing.

Ultra Fast Mass Spectrometry in Food & Beverages analysis

In mass spectrometry-based lipidomics, complex lipid mixtures undergo chromatographic separation, ionization, and detection using a high resolution, accurate mass (HRAM) instrument to simultaneously quantify and structurally characterize eluting species. The reported structural variety of identified lipids is strongly reliant on the analytical techniques utilized.In this webinar we describe a novel lipidomics Real-Time Library Search (RTLS) approach on the Orbitrap Ascend™ Tribrid mass spectrometer which utilizes the lipid class of real-time identifications to trigger class-targeted MSn and to improve the structural characterization of glycerophospholipids and sphingomyelins in positive ion mode. Our class based RTLS method demonstrates improved selectivity compared to current methodology, enhancing lipid characterization applications.Key learning objectives<ul><li>How recent technology advancements improve lipid analysis</li><li>What type of samples can be analyzed using on-the-fly mass spectrometry</li><li>Tips and tricks end users can employ to get more confident lipid annotation &nbsp;</li></ul><blockquote>Presenter: Dain Brademan, Ph.D. Postdoctoral Fellow (Coon Laboratory, Morgridge Institute for Research, University of Wisconsin–Madison)Dr. Brademan’s research focuses on how mass spectrometry data can be acquired, analyzed, and disseminated more efficiently. He has developed several software packages and web-based data visualization platforms which facilitate the analysis and interpretation of mass spectrometry data in a secure and intuitive environment.</blockquote>

Thermo Fisher Scientific: Structural Lipidomics Using Real-Time Library Search or, how Mass Spectrometry with on-the-fly Software Improves Phospholipid Characterization

Structural Lipidomics Using Real-Time Library Search or, how Mass Spectrometry with on-the-fly Software Improves Phospholipid Characterization

In this webinar we describe a novel lipidomics Real-Time Library Search (RTLS) approach on the Orbitrap Ascend™ Tribrid mass spectrometer 

Structural Lipidomics with Real-Time MS Library Search

<div>Submit the short form below to watch an exclusive webinar where you will:<ul><li>Overcome challenges—Discover innovative solutions for detecting complex biotransformation products.</li><li>Explore advanced techniques—See a novel high-resolution LC/MS strategy in action.</li><li>Gain practical insights—Watch demonstrations of the workflow applied to known drugs and their metabolites.</li></ul></div>

Agilent Technologies: Empower Drug Metabolite Identification

Empower Drug Metabolite Identification

Discover innovative LC/MS workflows for detecting complex biotransformation products with practical demos on drugs and metabolites.

<div><h4>Time to unlock a new level of confidence with multi-reflecting time-of-flight technology&nbsp;</h4>Join us for this exciting new demo series where we take you into our application laboratories to see the Xevo MRT in Action and explore different workflows from the beginning to the end. Register now for the demo series and see for yourself how Xevo MRT MS can expand the possibilities in your research.In this first session you will get an overview of the hardware from Jason Wildgoose, our lead engineer on the Xevo MRT project before we pass over to Rachel Sanig, one of our talented scientists who will give an overview of the data acquisition and processing software.<h5>Why Should You Attend?</h5><ul><li>Learn about the operation of the novel Xevo MRT MS technology and see it in Action!</li><li>Experience the benefits of a system that delivers mass resolution consistent over a broad m/z range independent of scan speed.</li><li>Discover ultimate flexibility with the all new &nbsp;DATA Convert application which converts data sets into the universal file format, mzML.</li></ul>The session will conclude with a live Q&amp;A where members of the Waters Xevo MRT team will be on hand to answer all of your questions.&nbsp;<blockquote>Presenter: Dr Jayne Kirk (Principal Consulting Product Manager, Waters Corporation)Jayne is a Product Marketing Manager at Waters with over 18 years’ experience in LC/MS instrumentation, technologies and software. She graduated from York University, UK, with a PhD in Analytical Chemistry and has previously held several scientific roles at Waters, developing applications and supporting customers. Her passion for translating technical information into impactful scientific messaging was the driving force behinds Jayne’s move to marketing.Presenter: Jason Wildgoose (Technology and Engineering Lead, Xevo MRT program, Waters Corporation)Jason Wildgoose joined Waters in early 1997 straight from university as a test and installation engineer working on the original QToF product. After 3 years he moved into the research and development team, continuing to work on time of flight technology and adjacent technologies such as ion guides, detectors, and ion mobility. He remained in research for 18 years and ultimately assumed responsibility for time of flight design &amp; development. In 2018 he joined the newly formed Waters Advanced MS Technologies team focusing on innovation of new technologies as the technical lead.Presenter: Rachel Sanig (Senior Scientist, Xevo MRT program, Waters Corporation)<div>Rachel Sanig received a 1st Class Masters and BSc (Hons) in Chemistry from the University of Leeds. UK. She joined Waters as an applications scientist in 2016 working across clinical, pharmaceutical, and chemicals and materials market areas using a range of LC-MS analysis techniques. In 2023 she joined the characterization team, where she works as a senior scientist on ToF and MRT technologies with LC and GC experience. She is also currently studying for a PhD in recycled plastics analysis.</div></blockquote></div>

Waters Corporation: XEVO MRT in Action - Demo Series

Unveiling the Xevo MRT: Hardware and Software Overview

Discover Xevo MRT MS with multi-reflecting TOF technology, delivering consistent resolution, flexible workflows, and data conversion to mzML.

Mastering Biopharmaceutical Characterization

<div><h4>Dive into real world applications with multi-reflecting time of flight technology</h4>Join us for in this second part of the Xevo MRT in Action series where we take you into our application laboratories explore different workflows from the beginning to the end for metabolite identification and PFAS analysis &nbsp;Register now for the demo series and see for yourself how Xevo MRT can expand the possibilities in your research.<h5>Why Should You Attend?</h5><ul><li>Learn about the operation of the novel Xevo MRT MS technology and see it in Action!</li><li>Experience the benefits of a system that delivers mass resolution consistent over a broad m/z range independent of scan speed.</li><li>Discover ultimate flexibility with the all new &nbsp;DATA Convert application which converts data sets into the universal file format, mzML.</li></ul>The session will conclude with a live Q&amp;A where members of the Waters Discovery MS portfolio science team will be on hand to answer all of your questions. &nbsp;<blockquote>Presenter: Isabel Riba (Principal Sales Applications Chemist, Waters Corporation)<div>Isabel is a pharmacist who obtained her PhD in Mass Spectrometry at UMIST, Manchester, UK. She has worked as a research scientist in university and pharmaceutical companies with focus on analytical chemistry and structural mechanisms for peptide fragmentation. In the last years she has worked at Waters Corporation in Manchester, as a demo chemist working on different applications for detection of small molecules, mainly using ion mobility mass spectrometry (IMS) as a orthogonal technique for more accurate compound identification.</div>Presenter: &nbsp;Dr. Hania Khoury-Hollins ( Principal Scientist, Waters Corporation)<div>Hania Khoury-Hollins is a scientist and researcher at Waters Corporation. She holds a bachelor’s degree in science, which laid the foundation for her scientific pursuits. In 2001, Hania earned her master’s degree in Toxicology, deepening her understanding of the impact of chemicals on living organisms. In 2005 she successfully defended her Ph.D. in physical chemistry from University René Descartes - Paris V (France). After undertaking several different projects in academia Hania spent some time working at Agilent before joining Waters.At Waters, her work revolves around developing cutting-edge analytical techniques and their applications with a focus on Liquid Chromatography Coupled with High-Resolution Mass Spectrometry (LC-HRMS). Her expertise encompasses a diverse range of applications, including drug development, proteomics, metabolomics, and lipidomics. More recently, Hania has been developing applications strategies for per- and polyfluoroalkyl substances (PFAS).</div></blockquote></div>

Insights into Small Molecule Applications

Discover Xevo MRT MS in action with metabolite ID and PFAS analysis, consistent high-resolution TOF, flexible workflows, and mzML data conversion.

LCMS webinars focusing on LC/HRMS - page 42