<div>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</div><div><div><div><div><h3>What is Protein Purification?</h3>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.This process is especially vital in biotechnological areas inside immunology and microbiology. In immunology, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In microbiology, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</div></div></div></div><div><div><div><div><h3>Types of Protein Purification Methods</h3></div></div></div></div><div>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</div><div><div><div><div><h4>Non-Chromatographic Approaches</h4></div></div><div>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.<h5>Precipitation Techniques</h5>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:<ul><li>Organic solvent precipitation involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li>Isoelectric point precipitation exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li>Nonionic polymer precipitation uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5>Dialysis</h5>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.<h5>Immunocapture (Magnetic Bead-Based Purification</h5>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:<ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.<h5>Centrifugation</h5>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.Preparative Ultracentrifugation Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.Differential Centrifugation Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.Rate-zonal Gradient Centrifugation This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.Isopycnic Centrifugation (Equilibrium Centrifugation) Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</div></div></div><div><div><div><div><h4>Chromatographic Approaches</h4>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.<h5>Ion Exchange Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526">Luna SCX </a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540">Biozen WCX</a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5>Size Exclusion Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.Phenomenex offers a range of Biozen columns for SEC separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534">Biozen dSEC-2</a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"> Biozen dSEC-7 </a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533">Biozen SEC-2</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532">Biozen SEC-3</a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.<h5>Reversed-Phase Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.<h5>Hydroxyapatite Chromatography</h5>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.<h5>Hydrophobic Interaction Chromatography</h5>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.<h5>Affinity Chromatography</h5>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

Article

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Application

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Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Separation Science

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Shimadzu Corporation

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Waters Corporation

In this webinar, Dr. Zijie “Beryl” Xia will provide a sneak peek inside the non-commercial, research driven PFAS testing lab at Claros Technologies. 
Beryl’s lab focuses on supporting the research and development of PFAS remediation and destruction at Claros Technologies, and has encountered some interesting problems, while learning some fun facts about PFAS in the process. 
Data from various methods on Waters Xevo TQ-Absolute will be shared, including both the standard methods and some customized approaches. The goal for this webinar is to share her experiences and knowledge about PFAS with the community.
For more information please contact Jenifer_Lewis@Waters.com or narendra_meruva@Waters.com
<blockquote>
Presenter: Dr. Zijie “Beryl” Xia (Director of the Analytical Laboratory and Service, Claros Technologies)
</blockquote>
<blockquote>
Dr. Zijie “Beryl” Xia obtained her Ph.D. in chemistry from University of California, Berkeley, with a focus in analytical and physical chemistry, especially in the field of mass spectrometry. She currently serves as the Director of the Analytical Laboratory and Service at Claros Technologies, offering both standard PFAS analysis as well as customized method development for non-standard compounds and diverse matrices, including non-potable water and textiles. Dr. Xia is an industry leader in PFAS analysis and is an active member of the ASTM International committee for PFAS method development.
</blockquote>
<blockquote>
Moderator: Jenifer Lewis (Senior Environmental Market Development Manager, Waters Corporation)
</blockquote>
<blockquote>
Jenifer Lewis is a Senior Market Development Manager with Waters Corp, focusing on theenvironmental market. She supports Waters’ business development teams in the Americas region,bringing over 30 years of experience in the environmental industry to assist with our customer’schallenges and needs. She has a bachelor’s degree in chemistry and an extensive background in GCand LC applications.
</blockquote>

Waters Corporation: Learnings from An Atypical PFAS Testing Lab

Learnings from An Atypical PFAS Testing Lab

In this webinar, Dr. Zijie “Beryl” Xia will provide a sneak peek inside the non-commercial, research driven PFAS testing lab at Claros Technologies. 

By attending this presentation, you will learn about:
<ul>
<li>The strategies for analyzing broad panels of pesticides with both LC/MS/MS and GC/MS/MS
</li>
<li>The approaches to address matrix interferences and consideration for instruments and methodologies
</li>
<li>Method validation requirements and accreditation criteria from the government perspective
</li>
</ul>
This presentation will review the laboratory perspective on the regulation and surveillance of approximately 270 pesticides and how this testing ties into federal government programs. Resulting methods used by the Ohio Department of Agriculture (ODA) Pesticide Residue laboratory show a lower quantitation limit at or below 10 parts per billion in complex matrices using a QuEChERS extraction paired with GC/MS/MS and LC/MS/MS. This work will summarize data found using these methods and compare goals, analysis requirements, and challenges between the USDA Agriculture Monitoring Service Pesticide Data Program and the U.S. EPA Region 5 with respect to pesticide use.
Additionally discussed will be strategies and challenges of using multiresidue methods on mass spectrometry platforms from the ODA including:
<ul>
<li>Workflow strategies
</li>
<li>Matrix interferences and cleanup
</li>
<li>Instrumental and methodological considerations, including compatibility of pesticides in these methods
</li>
<li>Method validation requirements and accreditation criteria
</li>
</ul>
<blockquote>
Presenter: Deidre E. Damon (Scientist and Mass Spectrometrist, Ohio Department of Agriculture’s Consumer Protection Laboratory)
</blockquote>
<blockquote>
Deidre Damon has been a scientist and mass spectrometrist for the Ohio Department of Agriculture’s Consumer Protection laboratory since 2019. She earned her PhD in analytical chemistry at the Ohio State University, focusing on alternative sampling and ionization sources for mass spectrometry. Since 2021, she has supervised the Pesticide Residue and Industrial Hemp laboratories where she oversees regulatory and surveillance testing of pesticides in environmental and food commodities on behalf of the U.S. EPA, USDA, and FDA for the state of Ohio.
</blockquote>

Separation Science - A Government Perspective on Pesticide Surveillance and Enforcement in Food and the Environment

A Government Perspective on Pesticide Surveillance and Enforcement in Food and the Environment

Learn about the strategies for analyzing broad panels of pesticides with both LC/MS/MS and GC/MS/MS

A Government Perspective on Pesticide Surveillance

Join us for an insightful webinar delving into the complexities surrounding nitrosamines in pharmaceutical products. Nitrosamines - known carcinogens, have emerged as a critical concern in the pharmaceutical industry, triggering stringent regulatory scrutiny. This webinar will explore the compendial standards, regulatory landscape, and analytical hurdles associated with detecting and mitigating nitrosamine contamination.
Gain invaluable insights from experts as we navigate the multifaceted challenges and discuss practical strategies for compliance and risk management.
Key Learnings:
<ul>
<li>Best practices for conducting risk assessment and testing for nitrosamine impurities
</li>
<li>How to assess the risk associated with Nitrosamines and developing control strategies
</li>
<li>Workflow for 17 Nitrosamines covering Sample Prep and LC-MS/MS method
</li>
<li>Control strategies aimed at addressing sensitivity, selectivity, and reproducibility issues with particular emphasis on sample preparation challenges
</li>
</ul>
<blockquote>
Presenter: Dr. Amanda Guiraldelli, Ph.D (Scientific Affairs Manager, United States Pharmacopeia)
</blockquote>
<blockquote>
Amanda Guiraldelli has been with USP since 2012 and holds the position of scientific affairs manager. She also serves as scientific liaison for the USP Measurement and Data Quality Expert Committee, where she works to develop and revise USP standards related to analytical procedure lifecycle/validation. Previously, Amanda worked as senior scientist at the USP reference standard laboratory for 8 years with characterization of compendial standards. She is visiting professor at the Institute of Chemistry at the University of Campinas (UNICAMP) in Brazil and is a frequent speaker and instructor on topics related to analytical procedure life cycle, AQbD and Chromatography.
</blockquote>
<blockquote>
Amanda is specialist in mass spectrometry, chromatography, omics and chemometrics and has more than 15 years of experience in pharmaceutical R&amp;D areas. Prior to joining USP, she was R&amp;D scientist in a Brazilian pharmaceutical industry and visiting scientist at TU Berlin in Germany and Leiden University in Netherlands (Center for Proteomics and Metabolomics) working on proteins characterization by LC-HRMS and procedure development using UHPLC-HRMS. Amanda is graduated in pharmacy biochemistry and holds a Ph.D. in analytical chemistry from the FCFRP - University of São Paulo (metabolomics by UHPLC-HRMS, GC-MS and 1H NMR and chemometrics).
</blockquote>
<blockquote>
Presenter: Mr. Naiffer Romero (MSc, MPH, United States Pharmacopeia)
</blockquote>
<blockquote>
Naiffer has more than 19+ years of pharmaceutical industry experience. In his 15 years with USP, he has served several roles: lead scientist in performance testing compendial reference standards development, Manager in charge of LATAM Compendial engagement and education with stakeholders and national regulatory bodies, and certified USP Education instructor.
</blockquote>
<blockquote>
Naiffer joined USP’s Scientific Affair performance cell in 2020, where he leads scientific outreach and technical engagement for LATAM &amp; US region on critical national health priority topics. His combined pharmaceutical expertise includes Analytical development, salt and polymorph selection, Development of dissolution methods, IVIVC modeling, and impurity analytical strategy. Naiffer is a recognized subject matter expert in Nitrosamines Impurities, and he also serves as a member of USP’s Nitrosamine workstream Committee and community manager to ‘Nitrosamine Exchange’, a knowledge community hosted by USP in All-things Nitrosamines Impurities.
</blockquote>
<blockquote>
Naiffer also liaises technical discussions on pharmacopeial collaboration, including the International Meeting of World Pharmacopeias (part of WHO), international compendial discussion groups, and several regional regulatory agencies’ nitrosamines working groups.
</blockquote>
<blockquote>
Presenter: Mr. Tobias Weimer (Produktspezialist LC &amp; LC/MSD, Agilent Technologies) 
</blockquote>
<blockquote>
Tobias hat in Marburg an der Philipps Universität Chemie studiert Seine Leidenschaft für die analytische Chemie und Chromatographie wurde bereits in der Oberschule geweckt und im Studium vertieft mit einer gezielten Ausrichtung auf analytische Trenntechniken. Über die Flüssigkeitschromatographie im Bereich LC Säulen führte Ihn sein Weg zu LC Systemen.
</blockquote>

Agilent: Unraveling Nitrosamines: Navigating Compendial, Regulatory, and Analytical Challenges

Unraveling Nitrosamines: Navigating Compendial, Regulatory, and Analytical Challenges

Webinar will explore the compendial standards, regulatory landscape, and analytical hurdles associated with detecting and mitigating nitrosamine contamination.

Unraveling Nitrosamines: Navigating Compendial, Regulatory

Human blood plasma contains thousands of distinct lipid species. These lipids can provide new insights into health and disease, with lipidomic profiling revealing new biomarkers for various conditions and being used to monitor the efficacy of treatments.
This webinar explores a new workflow for lipidomics using dia-PASEF – a mass spectrometry approach that allows for faster, more comprehensive analysis. Our expert speaker will show how this method increases lipid library coverage by 60% and allows you to capture complete lipid data in a single run. They will also unveil the optimal approach for high-throughput lipid analysis in human plasma, helping you to enhance your lipidomics research. 
Attend this webinar to:
<ul>
<li>Increase your lipid library coverage with dia-PASEF</li>
<li>Hear how to gain complete lipid data in a single run</li>
<li>Discover an optimized analysis scheme for high-throughput lipidomics</li>
</ul>
<blockquote>
Presenter: Dr. Hannes Röst (Assistant Professor and Canada Research Chair in Mass Spectrometry-Based Personalized Medicine at University of Toronto)
</blockquote>
<blockquote>
I am a bioinformatics researcher interested in high-throughput technologies that allow us to study the molecular phenotype of a biological system comprehensively. I have worked on theoretical questions in targeted proteomics, contributed to the development of SWATH-MS and wrote the first software capable of targeted analysis of SWATH-MS data in high throughput. I studied at ETH Zurich, Switzerland, worked with Ruedi Aebersold during my PhD and I am now working with Mike Snyder at Stanford University to apply mass spectrometry in a personalized medicine context.
</blockquote>

Technology Networks: Next-Generation Plasma Lipidomics: Quantification With dia-PASEF

Next-Generation Plasma Lipidomics: Quantification With dia-PASEF

New workflow for lipidomics using dia-PASEF – a mass spectrometry approach that allows for faster, more comprehensive analysis.

Plasma Lipidomics: Quantification With dia-PASEF

<h4>LC-MS/MS fundamentals webinar series 2.0</h4>
Advanced techniques for data acquisition and processing
Elevate your analytical expertise with advanced techniques and workflows
Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series. Based on our LC-MS/MS 1.0 webinar series, these sessions will go beyond the fundamentals of LC-MS/MS and introduce ways to advance your analytical methods and data processing workflows.
Every other week, we will explore how to optimize SCIEX OS software with short, 30-minute expert-led presentations.
<h4>This webinar:</h4>
May 7, 2024 11:00 a.m. - 11:30 a.m. CET - Beyond the fundamentals
<h4>Other webinars from the series:</h4>
May 21, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3852">Advanced MS/MS method building</a>
Gain insight into advanced features for maximizing sensitivity in your mass spectrometry analyses.
June 4, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3853">Mastering chromatography</a>
Discover how to optimize your chromatography for even the most challenging compounds while creating and building LC methods.
June 18, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3854">It&#39;s a TRAP! Getting the most out of your QTRAP system</a>
Learn when to utilize QTRAP functionality to create more selective and sensitive methods.
July 2, 2024 11:00 a.m. - 11:30 a.m. CET- <a href="https://lcms.labrulez.com/webinar/3855">Troubleshooting at the source</a>
Acquire the knowledge needed to optimize source parameters and identify and resolve ionization-related problems.
July 16, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3856">Automating data processing</a>
Learn how to automate your data processing with custom flagging and rules for efficient data review in SCIEX OS software.
July 30, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3857">Excel strategies for mass spectrometry data analysis</a>
Take an in-depth look at when and how to use Excel to increase your productivity.

SCIEX: LC-MS/MS fundamentals webinar series 2.0

Beyond the fundamentals

Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series.

Beyond the LC-MS/MS fundamentals

<ul>
<li>Gain insights into current food legislation relating to PFAS
</li>
<li>Learn about targeted and untargeted approaches for PFAS analysis using LC-MS/MS
</li>
<li>Uncover the capabilities of the LCMS PFAS in eggs analysis with online SPE clean-up
</li>
<li>Find out about the benefits of automated and &quot;ready-to-use&quot; methods
</li>
</ul>
<blockquote>
Presenter: Jonathan McGeeha (Food Safety Market Specialist, Shimadzu)
</blockquote>

PFAS in Food – A not so “eggs-cellent” discovery! Analytical approaches for PFAS in food

Join the webinar to learn more about analytical approaches into analysing PFAS in food.

Analytical approaches for PFAS in food

Recognizing the importance of cellular metabolism and the complexity of cell-cell heterogeneity, SpaceM has emerged as a promising technology with high potential for investigating single-cell metabolomics. SpaceM is expected to grant broad access to single-cell metabolomics, enabling investigations across diverse cellular models. Additionally, addressing the challenge of reproducibility, we present HT-SpaceM, a high-throughput method showcasing robust reproducibility in identifying metabolites in 10 cell lines, highlighting the promise of the SpaceM method in understanding cellular metabolism and disease.
<blockquote>
Presenter: Shawn Owens, Ph.D. (Imaging Mass Spectrometrist, SpaceM, BioInnovation Institute, Copenhagen, Denmark)
</blockquote>
<blockquote>
Shawn began his mass spectrometry imaging journey over 10 years ago, at the University of California- Santa Barbara- where he earned his PhD. With his degree in hand, he began a journey that led him through roles in industry- first at Bruker for nearly 5 years, followed by a year at BASF in New York. Eager to explore new horizons, Shawn embarked on a transformative journey in a startup, joining SpaceM in Copenhagen, Denmark- where he assumed the role of R&amp;D Team Leader and Mass Spectrometrist, a role he has held for the past 18 months.
</blockquote>

Bruker: High Throughput Single-Cell Metabolomics

High Throughput Single-Cell Metabolomics

We present HT-SpaceM, a high-throughput method showcasing robust reproducibility in identifying metabolites in 10 cell lines.

<h3>PFAS and us: The science</h3>
Driving change for a healthier world
Webinar series
<h4>Enrich your understanding of PFAS chemicals and their impact</h4>
Stay on the leading edge of all things PFAS with this enlightening monthly webinar series on testing workflows and cutting-edge technology that will demystify these “forever chemicals”.
Each month, in these 30-minute sessions, industry thought leaders unite to share the current state of PFAS testing and the far-reaching impact that PFAS have on human health and the environment. Build on your current knowledge of testing methodologies and research techniques that will help you develop your own innovative strategies for precise identification and quantitation. Make a lasting impact and join us for these conversations.
<h4>This Webinar:</h4>
May 8, 2024 10:00 a.m. PT/ 1:00 p.m. ET - Rapid PFAS testing with no compromises: direct-inject analysis
<blockquote>
Presenter: Jamie Fox (PFAS Technical Program Manager, Enthalpy Analytical)
</blockquote>
<h4>Other Webinars from the Series:</h4>
April 10, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3865">Examining the exogenous: PFAS and beyond</a>
<blockquote>
Presenter: Andrew Patterson (Vice President, Innovation and Development, Eurofins Environmental Testing America)
</blockquote>
June 12, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3867">Assessment of advanced analytical instruments to reach required limits of quantitation for PFAS in foods</a>
<blockquote>
Presenter: Susan Genualdi, PhD (Research Chemist, FDA, Center for Food Safety and Applied Nutrition, Office of Regulatory Science)
</blockquote>
July 17, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3868">Detecting and identifying known and novel per/polyfluoroalkyl substances (PFAS) in a marine apex predator</a>
<blockquote>
Presenter: Carrie McDonough (Assistant Professor, Chemistry, Carnegie Mellon University)
</blockquote>
August 14, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3869">Whole blood microsampling methods for PFAS exposure biomonitoring</a>
<blockquote>
Presenter: Andrew J. Morris, PhD (Professor, University of Arkansas for Medical Sciences, Research Career Scientist, Central Arkansas Veterans Affairs Healthcare System)
</blockquote>
September 11, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3870">Analysis of ultra short-chain PFCAs to better understand environmental sources and fate</a>
<blockquote>
Presenter: Cora J. Young (Associate Professor, Department of Chemistry, York University)
</blockquote>

Rapid PFAS testing with no compromises: direct-inject analysis

Stay on the leading edge of all things PFAS with this enlightening monthly webinar series.

Rapid PFAS testing: direct-inject analysis

<h4>Virtual event: Pharma conclave series 2024</h4>
SCIEX offers the platform for the experts to take the stage and drive technological progress
Welcome to Pharma conclave series 2024 – where our customers’ expertise meets innovation, transforming your knowledge and empowering you to revolutionize your work processes. Join us in discovering techniques that will enhance your skills to accelerate your daily operations in the development of new drugs.
From the research of new markers to the drug development moving into non and GLP bioanalysis. Confidently plan your next lab expansion with the knowledge gained from industry leaders and pioneers.
<ul>
<li>May 16 Event 1: Pharma omics</li>
<li>September 17 Event 2: Impurities</li>
<li>October 23-24 Event 3: Pharma</li>
</ul>
10:00 a.m.–12:00 p.m. CET
<h3>Event 1: Pharma omics</h3>
Lost in translation? Translational research meets drug discovery and development – 3rd edition
<h5>Topics covered:</h5>
<ul>
<li>‘Pathophysiological subtypes of mild cognitive impairment due to Alzheimer’s disease identified by CSF proteomics’ by Bruno Manadas, at CNC
</li>
<li>‘Scalable and automated workflows for global plasma proteomics by standardized LC-MS’ by Dorte Bekker-Jensen, at Evosep
</li>
<li>‘Quantitative, standardized and reproducible metabolomics solutions in pharmaceutical R&amp;D’, by Stefan Ledinger and Markus Langsdorf, at Biocrates Life sciences
</li>
<li>Multi-omics analysis on mouse plasma in a pre clinical development of a drug in Alzheimer
</li>
</ul>
<blockquote>
Presenter: Bruno Manadas, PhD
</blockquote>
<blockquote>
Dr Manadas is a prominent Cell Biology researcher at CNC-UC since 2017. With expertise in biochemistry, psychology, and biotechnology, he&#39;s authored 130+ publications and led multiple projects. As head of CNC&#39;s Mass Spectrometry node and Associate Editor for Frontiers, he&#39;s contributed significantly to neuroscience and psychiatry. Bruno&#39;s research focuses on cell biology mechanisms, translational biomarker research, and MS-based approaches for studying protein oxidation. He played a pivotal role in establishing the hospital biobank and teaches at the Portuguese Catholic University.
</blockquote>
<blockquote>
Presenter: Dorte Bekker-Jensen
</blockquote>
<blockquote>
Dorte is heading the Applications Development team in Proteomics Research at Evosep. She has long-term experience within proteomics and has focused on technology development by developing simple and automated workflows for high-throughput applications.
</blockquote>
<blockquote>
Presenter: Stefan Ledinger
</blockquote>
<blockquote>
Stefan has 20 years of experience in the life sciences and diagnostic industries. For the last dozen years, he has been serving various functions within biocrates life sciences ag. Most recently, he has been responsible for the market development for metabolomics solutions in the pharmaceutical and biotech industries. He currently serves as Global Head of Sales. Stefan holds degrees in business administration and bioengineering, combining commercial know-how with a passion for science.
</blockquote>
<blockquote>
Presenter: Markus Langsdorf
</blockquote>
<blockquote>
Markus has 14 years of experience in life sciences industries. As he joined biocrates in 2010, he worked for 11 years as application support specialist for biocrates’ metabolomics profiling solutions. 3 years ago he changed his position withing the company to his new function as product manager. Markus studied chemistry and graduated with a PhD in Analytical Chemistry.
</blockquote>

Pharma conclave series 2024: Pharma omics

Discover techniques that will enhance your skills to accelerate your daily operations in the development of new drugs.

Advances in enzymatic digestion techniques combined with new software analysis tools that incorporate in silico digestion capabilities for mRNA sequences, demonstrate the potential for sequence mapping and confirmation of mRNA using high-resolution LCMS. These new advances have the potential to progress mRNA sequencing by LCMS into the same well-defined workflow currently in place for the peptide mapping of therapeutic proteins.
Dr. Ken Cook, senior European team leader for the biopharma/pharma expert support group at Thermo Fisher Scientific, and Keeley Murphy, software product manager at Thermo Fisher Scientifc, will discuss the use of an automated workflow which includes enzymatic digestion, high-resolution LCMS analysis, and data analysis for mRNA sequencing.
Key learning objectives
<ul>
<li>Discover a new automated workflow for mRNA sequencing by high-resolution LCMS</li>
<li>Learn how to implement routine sequence confirmation of mRNA using high-resolution LCMS</li>
<li>Explore new software analysis tools that incorporate in silico digestion capabilities for mRNA sequences</li>
</ul>
Who should attend?
<ul>
<li>Biopharmaceutical research scientists</li>
<li>Analytical scientists from biopharma companies working on or interested in mRNA based therapies</li>
<li>PhD students in any field of biotherapeutic drug development</li>
<li>Scientists looking to advance their knowledge of oligonucleotide and mRNA analysis</li>
</ul>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Dr. Ken Cook (Senior European team leader for the biopharma/pharma expert support group, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Keeley Murphy (Software product manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Moderator: Moderator: Dora Wells (Editoral Team, SelectScience)
</blockquote>

SelectScience: Automated Workflow for mRNA Sequencing by High-Resolution LCMS

Automated Workflow for mRNA Sequencing by High-Resolution LCMS

We will discuss the use of an automated workflow which includes enzymatic digestion, high-resolution LCMS analysis, and data analysis for mRNA sequencing.

LCMS webinars focusing on LC/MS/MS - page 50