Ask any analytical chemist which hyphenated technique has become indispensable over the past decade, and the answer will almost certainly be the same: tandem chromatography–mass spectrometry. Whether it is <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis">LC–MS/MS</a> quantifying a novel biologic’s metabolic fate, or <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis">GC–MS/MS</a> chasing per- and polyfluoroalkyl substances (PFAS) down to sub-part-per-trillion levels in municipal water, the combination of chromatographic separation with the specificity of tandem mass detection has become the analytical workhorse of choice across virtually every regulated sector.Below we examine the key forces propelling this expansion, from biopharma pipeline dynamics and PFAS regulatory mandates to AI-driven automation and reshoring of pharmaceutical manufacturing—and explore what each trend means for laboratories managing sample preparation and analytical workflows.<h3>Biopharma: The Engine Room of LC–MS/MS Demand</h3><h4>GLP-1 Agonists and the Metabolomics Gold Rush</h4>The explosive commercial and scientific interest in GLP-1 receptor agonists—<a target="_blank" rel="noopener noreferrer" href="https://www.sepscience.com/advancing-glp-1-peptide-analysis-chromatography-challenges-and-innovations-12163">semaglutide and related peptide therapeutics </a>for diabetes and obesity—has dramatically expanded the analytical demands placed on tandem MS platforms. Discovery programs require precise quantification of peptide drug candidates and endogenous biomarkers across complex biological matrices. These workflows rely on high-sensitivity LC–MS/MS for pharmacokinetic profiling, receptor occupancy studies, and metabolomics characterization. Metabolomics is, in fact, projected to be the fastest-growing application segment in mass spectrometry, driven in part by its growing role in cancer screening, biomarker discovery, and metabolic disease research.I have been spending more time learning about GLP-1s because this is the hot topic that people want to discuss on the Concentrating on <a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL?si=n9GLz9zjSCOrVfXPxFPhng&amp;nd=1&amp;dlsi=52868e027aad4d22">Chromatography podcast</a>. With that said, I was astounded looking at CNBC.com during breakfast this morning to see that <a target="_blank" rel="noopener noreferrer" href="https://www.cnbc.com/2026/03/21/glp-1-diets-restaurants-protein-fiber-weight-loss-drugs.html">one in eight Americans are currently taking one of these drugs such as Ozempic</a>.<h4>Antibody–Drug Conjugates and Gene Therapy: Complexity at Every Step</h4>The ascent of antibody–drug conjugates (ADCs) and gene therapies has introduced a new level of analytical complexity. ADCs blend the target specificity of monoclonal antibodies with the cytotoxic potency of small-molecule payloads—and characterizing the linker chemistry, drug-to-antibody ratio (DAR), and conjugate stability demands high-resolution LC–MS/MS at every stage from discovery through QC release testing. Gene therapy vectors require equally rigorous characterization of impurities, aggregates, and empty/full capsid ratios.This translates into a direct need for low-evaporation, high-recovery concentration methods that preserve labile protein structures and maintain sample integrity prior to LC–MS/MS injection. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">Nitrogen evaporation workflows</a> that minimize oxidative stress and maintain sample temperature are increasingly critical in these pipelines.<h4>Precision Medicine and Multi-Omics Integration</h4>The shift toward precision medicine has accelerated adoption of high-throughput, multi-analyte MS platforms. Proteomics, metabolomics, and lipidomics workflows are converging on a single analytical platform—tandem MS—and generating datasets of a scale and complexity that were unimaginable ten years ago.Make no mistake: the triple quadrupole serves an irreplaceable role in quantitative targeted analysis. At the same time, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=134&amp;manufacturerItems=3">Orbitrap </a>and Q–TOF hybrid platforms are growing rapidly as researchers demand full-scan accurate mass for untargeted discovery.<h3>Environmental Mandates: PFAS Testing Drives Adoption</h3>Environmental testing is something that every person, chemist or not, can understand the importance of. Mounting mandates from the U.S. Environmental Protection Agency and European regulators regarding PFAS—the “forever chemicals” whose extraordinary environmental persistence is matched only by the analytical challenge of detecting them at trace levels—have created immediate, compliance-driven investment in LC–MS/MS infrastructure.<h4>The Regulatory Framework Chemists Need to Know</h4>The EPA has codified a suite of LC–MS/MS-based methods as the analytical standard for PFAS in water and solid matrices:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/epa-533-drying-down-pfas-samples-after-spe">EPA Method 533</a>: Weak anion exchange SPE combined with LC–MS/MS; covers 25 PFAS including short-chain compounds in drinking water.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/sample-concentration-epa-method-537-pfas-determination">EPA Method 537.1</a>: Reversed-phase SPE followed by LC–MS/MS; covers 18 PFAS including the GenX compound, with method detection limits in the low ng/L range.</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/en/solutions/environmental/water-testing/pfas-in-water/us-epa-method-8327?srsltid=AfmBOooCY1pcavj7B1PH-75d3HO4BGDglNlhmyTyrpDY3zvUksMO1dlg">EPA Method 8327</a>: LC–MS/MS for selected PFAS in prepared extracts of aqueous and solid matrices.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/preparing-diverse-sample-matrices-for-epa-method-1633">EPA Draft Method 1633</a>: The emerging benchmark for PFAS in non-aqueous matrices—aqueous, solid, biosolids, and tissue—quantifying up to 40 PFAS.</li></ul>All of these methods designate LC–MS/MS, specifically LC coupled to triple quadrupole or tandem-in-time platforms operating in negative electrospray ionization mode with multiple reaction monitoring (MRM), as the required or recommended analytical technique. PFAS analysis is, at its core, an LC–MS/MS problem.<h4>The Sample Preparation Bottleneck</h4>Chemists who have worked with PFAS methods understand the particular demands they place on sample preparation. The ubiquity of<a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/tackling-pfas-contamination-practical-insights-from-epa-537.1-method-development"> PFAS in laboratory materials</a>—PTFE tubing, some SPE sorbents, standard bench supplies—means that blank contamination control is as analytically demanding as the detection itself. EPA 537.1 requires that sample volumes of approximately 250 mL be extracted and reconstituted in as little as 1 mL, creating a 250:1 concentration factor that can expose even trace contamination in the sample processing chain. The installation of a delay column between the LC pump and autosampler to prevent instrument background interference is now standard practice.This is precisely where robust, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5385">inert nitrogen evaporation </a>systems earn their keep. PFAS workflows that use nitrogen-assisted evaporation to concentrate extracts prior to reconstitution benefit from materials that avoid PTFE and other fluoropolymers that can leach PFAS background—a consideration Organomation addresses in its concentration system designs.The analytical community is also moving beyond targeted PFAS analysis. High-resolution accurate mass (HRAM) spectrometry via Orbitrap technology, paired with non-targeted screening workflows, is gaining traction for the identification of the more than 5,000 currently catalogued PFAS compounds and the novel structures that continue to emerge. LC–MS/MS remains the quantitative gold standard; HRAM provides the discovery layer.<h3>Technological Trends Reshaping the LC–MS/MS Workflow</h3><h4>Artificial Intelligence and Automated Data Interpretation</h4>The datasets generated by modern tandem MS platforms—particularly in omics applications—are too large and too complex for conventional manual review. AI-driven spectral deconvolution, automated peak integration, and AI-assisted hypothesis generation are now embedded in the software suites accompanying leading instruments. <a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/about/newsroom/presrel/2022/17feb-gp22004.html?srsltid=AfmBOop3warRIaEojXM2YyMML6RXfMIDzE-Wm0h-SiKkkocBeeET2m7u">Agilent’s acquisition of Virtual Control’s ACIES </a>software specifically to automate LC–MS and GC–MS data analysis is emblematic of this direction.For laboratory managers, AI integration means smaller specialized teams can operate high-throughput multi-analyte workflows—but it also means that the front-end sample preparation must be even more reproducible, since algorithmic interpretation amplifies inconsistencies introduced upstream.<h4>Online SPE Coupling and “Chromatography Without Walls”</h4>A significant trend is the integration of solid-phase extraction directly into the HPLC flow path—so-called online SPE–HPLC coupling. Systems that combine extraction and chromatographic separation in a single analytical instrument reduce manual transfer steps, minimize the risk of sample loss or contamination, and dramatically increase throughput. For analytes at very low concentrations, online SPE provides the on-column concentration capability that traditional offline preparation achieves with manual evaporation steps.That said, offline sample preparation—including <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">nitrogen-assisted evaporation</a> under controlled conditions, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2407">liquid–liquid extraction</a>, and protein precipitation—retains significant advantages in flexibility, matrix handling, and the ability to process sample volumes that exceed online system capacity. The two approaches are increasingly complementary rather than competitive in well-designed analytical laboratories.<h4>High-Purity Solvents and Reference Materials</h4>As detection limits push further into the femtogram range and regulatory methods demand demonstrable blank performance, the quality of analytical consumables—mobile phase solvents, derivatization reagents, reference standards—has moved from background consideration to front-of-mind. Method validation in regulated environments now routinely requires vendor certification of solvent purity at a level that directly interfaces with instrument performance.<h3>The Ever-Present Need for Sample Evaporation</h3>As LC–MS/MS methods push detection limits lower, the analytical burden shifts increasingly upstream. The quality of the concentration, cleanup, and reconstitution steps preceding injection determines whether the instrument’s theoretical sensitivity is achievable in practice.Several specific developments are particularly relevant:<ul><li>ADC and gene therapy characterization workflows require gentle, low-temperature evaporation that preserves protein structure and prevents aggregation prior to LC–MS/MS. Nitrogen evaporation under controlled conditions is the preferred approach.</li><li>PFAS trace analysis demands evaporation systems constructed from PFAS-inert materials, with rigorous attention to avoiding fluoropolymer surfaces throughout the sample path.</li><li>High-throughput pharmaceutical QC driven by reshoring demands parallel processing capability—multi-position evaporators that maintain consistent flow and temperature across all positions simultaneously.</li><li>Multi-omics and metabolomics workflows generate large sample batches that benefit from automated, walk-away evaporation with programmable endpoints to ensure reproducible final volumes across a cohort.</li></ul>Tandem chromatography–mass spectrometry is not merely growing; it is becoming structurally embedded in the analytical workflows of every major regulated industry. The convergence of biopharma complexity, PFAS regulatory mandates, AI-enabled data analysis, and pharmaceutical reshoring is creating a multi-year demand cycle that will require laboratories to invest not only in instruments, but in the entire sample preparation ecosystem that makes those instruments perform.For analytical chemists, the practical takeaway is this: as mass spectrometry sensitivity continues to advance, the sample preparation step becomes the binding constraint on method performance. Whether you are developing a new PFAS method to meet EPA 1633, characterizing an ADC’s drug-to-antibody ratio, or running a 500-sample metabolomics cohort overnight, the reliability and cleanliness of your concentration and cleanup workflow determines the quality of every data point that follows.&nbsp;

The Continued Rise of Tandem Chromatography–Mass Spectrometry

News from LabRulezLCMS Library - Week 27, 2026

🎤Dr. Christopher Reid (Professor of Chemistry and Biochemistry at Bryant University in Rhode Island)In this episode of Concentrating on Chromatography, host Dave Oliva sits down with Chris Reid to explore how chemical biology and LC-MS are being used to tackle one of the most urgent global challenges: antimicrobial resistance.As antibiotic resistance continues to rise—and new drug development struggles to keep pace—researchers are looking beyond traditional approaches. Instead of targeting how bacteria build their cell walls, Chris’s work focuses on how they break them down, opening the door to entirely new antimicrobial strategies.<h4>What You’ll Learn in This Episode:</h4><ul><li>Why antimicrobial resistance is accelerating globally</li><li>What peptidoglycan is and why it’s essential to bacterial survival</li><li>How targeting cell wall degradation differs from traditional antibiotics</li><li>The strategy behind designing small molecule probes and inhibitors</li><li>How LC-MS is used to analyze peptidoglycan fragments and enzyme activity</li><li>Key analytical challenges: complex mixtures, low-abundance species, and structural similarity</li><li>The importance of sample preparation and reproducibility in LC-MS workflows</li><li>How this research could lead to new antibiotic targets</li></ul><h3>Video Transcription</h3><h4>The Growing Challenge of Antibiotic Resistance</h4>According to Reid, antibiotic resistance is increasing across a broad spectrum of bacterial pathogens that were once readily treated with existing therapies. In some cases, clinicians must now rely on only a handful of last-line antibiotics, while resistance is also rising in diseases such as tuberculosis.One of the major obstacles is the limited commercial incentive to develop entirely new antibiotics. Because bacterial resistance can emerge relatively quickly after a drug reaches the market, pharmaceutical companies often favor modifying existing antibiotics or combining them with resistance-modifying agents rather than investing in completely new drug classes. As a result, genuinely novel antibacterial targets remain urgently needed.<h4>Why the Bacterial Cell Wall Remains an Attractive Target</h4>The bacterial cell wall consists primarily of peptidoglycan, a highly cross-linked polymer that provides structural integrity, maintains cell shape, and enables bacterial growth and division.Many widely used antibiotics—including the β-lactam antibiotics such as penicillins—target enzymes responsible for synthesizing or cross-linking peptidoglycan. These pathways have proven highly successful therapeutic targets because peptidoglycan is unique to bacteria and absent from human cells, minimizing unwanted effects on the host.However, most existing antibiotics target only specific stages of peptidoglycan biosynthesis. Reid's research explores another part of this complex pathway: enzymes responsible for degrading the carbohydrate backbone of peptidoglycan during normal bacterial growth and remodeling. These enzymes may represent an underexplored class of antimicrobial targets capable of overcoming existing resistance mechanisms.<h4>Targeting the Glycan Backbone</h4>A key advantage of focusing on the carbohydrate backbone is its remarkable conservation across both Gram-positive and Gram-negative bacteria.Whereas the peptide components of peptidoglycan vary considerably between bacterial species—and frequently acquire mutations associated with antibiotic resistance—the glycan backbone remains highly conserved and tolerates relatively few structural modifications.This biological constraint makes enzymes acting on the glycan backbone particularly attractive targets. Reid's group developed a series of small-molecule probes and inhibitors designed to recognize enzymes known as N-acetylglucosaminidases, which cleave glycosidic bonds within peptidoglycan and generate soluble degradation products.Rather than beginning with purified enzymes, the researchers adopted a top-down phenotypic screening strategy. Candidate compounds were first evaluated for their ability to inhibit bacterial growth before progressively narrowing the list of potential molecular targets through genetic studies, microscopy, enzyme assays, and biochemical characterization. This iterative workflow ultimately identified compounds capable of inhibiting the desired enzyme class while simultaneously providing valuable chemical probes for studying bacterial cell wall biology.<h4>LC-MS as a Central Analytical Tool</h4>Liquid chromatography coupled with mass spectrometry plays a central role throughout the project by enabling detailed characterization of structural changes occurring within peptidoglycan after bacterial cells are treated with candidate inhibitors.Because intact peptidoglycan is an insoluble polymer, sample preparation begins with isolation of the bacterial cell wall through detergent extraction and differential centrifugation. Additional purification steps remove proteins, nucleic acids, and teichoic acids before enzymatic digestion converts the insoluble material into soluble fragments suitable for chromatographic analysis.The resulting digest is analyzed by LC-MS to examine changes in the distribution of peptidoglycan fragments. Chromatographic retention times provide an immediate indication of changes in cross-linking, with larger oligomers such as trimers and tetramers generally eluting later than monomeric fragments. Mass spectrometry then confirms the identity of these fragments by measuring their intact molecular masses, while tandem MS experiments can provide further structural information when required.<h4>Sample Preparation Determines Data Quality</h4>Reid emphasized that sample preparation represents one of the most critical—and challenging—stages of the entire workflow.Because peptidoglycan analysis involves multiple purification, digestion, and reduction steps, even minor procedural differences between samples can introduce analytical artifacts that complicate biological interpretation.Maintaining strict consistency throughout sample preparation is therefore essential. Appropriate controls must accompany every experiment, ensuring that observed chromatographic and mass spectrometric differences truly reflect biological responses rather than experimental variability. The complexity of bacterial cell wall composition further increases the analytical challenge, as subtle differences in amino acid substitutions and low-abundance cross-linked species can complicate LC-MS data interpretation.<h4>Chemical Biology for Future Antibiotic Discovery</h4>Although the identified compounds may eventually serve as starting points for antibiotic development, Reid views them primarily as chemical biology tools.By selectively inhibiting individual enzymes, these probes allow researchers to investigate previously unexplored aspects of bacterial cell wall remodeling that cannot easily be studied using classical genetic approaches alone.The research team is now expanding this strategy by combining their probes with proximity-labeling proteomics and mass spectrometry, aiming to map protein interaction networks associated with bacterial cell wall degradation. Such studies may reveal entirely new antibacterial targets and provide deeper insight into the biological processes governing bacterial growth and division.Ultimately, the work illustrates how integrating synthetic chemistry, microbiology, molecular biology, and advanced LC-MS can accelerate the search for innovative therapeutic strategies against antimicrobial resistance. As existing antibiotics become progressively less effective, multidisciplinary approaches such as these may play an increasingly important role in identifying the next generation of antibacterial targets.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

 Fighting Antibiotic Resistance with LC-MS: Targeting Bacterial Cell Walls

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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Agilent Technologies

Phenomenex

Thermo Fisher Scientific

Shimadzu Corporation

Waters Corporation

When we encounter unexpected things with our chromatography, we wonder why did that happen? As we look at these problems, we’ll also take a closer look at our sample and mobile phase to better understand how they may be contributing to our chromatography problems.
A better understanding of where problems can come from helps us narrow down the possibilities, solve our problem faster, and identify ways to prevent them from happening in the future.
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Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
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<blockquote>
Golnar Javadi has a Master’s degree in Chemical Engineering with more than 20 years of experience in the field of analytical chemistry. Prior to her current role, she taught Chemistry at college level for 5 years and worked in the pharmaceutical and nutraceutical industries where she obtained firsthand experience with analytical method development and sample preparation techniques.
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Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
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WHY Did That Happen?

This webinar will look at available resources to help meet your chromatography needs like:
<ul>
<li>Who can help me with method development questions, like what column to choose?</li>
<li>How can I get help with troubleshooting?</li>
<li>Where can I find videos to help with my instrument maintenance?</li>
<li>Is there a forum where I can ask other chromatographers for their input?</li>
</ul>
We’ll talk about these and other resources to help you be successful.
<blockquote>
Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Golnar Javadi has a Master’s degree in Chemical Engineering with more than 20 years of experience in the field of analytical chemistry. Prior to her current role, she taught Chemistry at college level for 5 years and worked in the pharmaceutical and nutraceutical industries where she obtained firsthand experience with analytical method development and sample preparation techniques.
</blockquote>
<blockquote>
Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
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Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
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Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
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Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)
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Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.
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Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
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Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
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Presenter: Paul Altiero (Application Engineer, Agilent Technologies, Inc.)
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Paul Altiero is an Applications Engineer located at the Agilent Little Falls site in Wilmington, DE. Prior to coming to Agilent in 2009, Paul worked in the pharmaceutical industry performing LCMS bioanalysis supporting Drug Discovery DMPK. While at Agilent, Paul has been a field service engineer for LCMS as well as the integrating program manager for the RapidFire high throughput LCMS system. In his current role Paul’s responsibilities include technical support and application assistance for LCMS consumables.
</blockquote>

Agilent Technologies: WHO Do I Turn To? Chromatography Resources

WHO Do I Turn To? Chromatography Resources

Cultivation-independent sequencing techniques have revealed a tremendous diversity of microorganisms on our planet. Although, the majority of microorganisms are not isolated and do not have sequenced genomes. While this applies to all fields of environmental microbiology, it does not prevent research from progressing. Conversely, in the field of subsurface microbiology the dearth of cultured representatives heavily restricts the simplest hypothesis driven experimentation.
In this work, we discovered a novel strict anaerobic thermophilic bacterium from a monitoring borehole at a depth of 691 meters below land surface. This borehole is of particular interests as it resides within the US Great Basin, in particular the Nevada National Security Site, underlain by the Death Valley Regional Flow System. The characterized isolate was given the name Thermoanaerosceptrum fracticalcis strain DRI-13T, and the sequenced genome coupled with methylome analysis reveal interesting insights into metabolism/energy conservation, gene regulation, DNA defences, and archival of previous viral aggressions. This microorganism and others have been analysed using HPLC to track substrate utilization and waste product production in order to better understand the syntrophic interactions of subsurface life. The deep biosphere may reveal itself as an untapped resource of microbial biodiversity holding novel solutions to industrial, medical, and potential providing answer to the origins of life.
Our panel will host a Q&amp;A session after the presentation to address any questions or comments. 
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Presenter: Scott D. Hamilton-Brehm, Ph.D. (Assistant Professor Department of Microbiology, Southern Illinois University)
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Scott Hamilton-Brehm received his B.S. (1997) from San Louis Obispo in San Louise Obispo, CA. where he studied bacteria inside the abdomens of prehistoric bees trapped in amber. He worked for the biotech company Promega Biosciences for 4 years before returning to academia. He received his Ph.D. in biochemistry and molecular biology (2008) from University of Georgia (UGA) in Athens, GA. At UGA he studied the model hyperthermophilic archaeon Pyrococcus furiosus and how it reacted to iron-sulfur-nitrogen based antibiotic molecules. He discovered his talent and skill of culturing thermophilic microorganisms. This skill set led him to work as a Postdoctoral Research Associate (2008-2012) at Oak Ridge National Laboratory (ORNL). At ORNL he work for the BioEnergy Science Center (BESC) whose mandate was to research the application microbial mediated consolidated bio-processing (CBP) to generate biofuels. Afterwards, Scott was part of the Division of Earth and Ecosystem Sciences (DEES) at the Desert Research Institute in Las Vegas, NV (2012-2016), where he studied and cultivated strict anaerobic thermophilic microbial communities from the deep subsurface of the Southern Nevada Desert. Now he is part of the Microbiology Department at Southern Illinois University of Carbondale (SIUC), where he is continuing his research into culturing deep subsurface microbes and bacterial driven biofuel production. During the beginning of the 2020 COVID-19 pandemic, Chicago was fighting against becoming a new hot spot for the virus, testing in Illinois slowed when the availability of viral transport medium (VTM) became strained. The governor of Illinois made an emergency call to university researchers to make VTM in an effort protect the state’s citizens. Hamilton-Brehm’s laboratory rallied, pulling support from the departments of microbiology and zoology at SIUC to answer the governor’s call. Professors and student volunteers together produced 115K vials of VTM for the state of Illinois over two months.
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Presenter: Mathew Chaidez B.S. (Field Sales Engineer, Shimadzu)
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<blockquote>
<ul>
<li>4 years in the 82nd Airborne Division from 2000-04</li>
</ul>
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<ul>
<li>B.S. in Chemistry from Saint Louis University</li>
<li>5 years analytical experience in a radiopharmaceutical lab</li>
<li>5 years sales with Shimadzu</li>
</ul>

Shimadzu: Subsurface Microbiology: Metabolism by HPLC

Subsurface Microbiology: Metabolism by HPLC

Agilent’s MassHunter WalkUp simplifies routine assays that allows all users access to instrumentation for their samples. WalkUp is a software interface with predefined users and acquisition methods for both LC and LC/MS instrumentation.
Here we will provide an introduction to WalkUp briefly discussing the user experience from the administrator (setting up groups, users, methods, data analysis confirmation) and users’ (running samples) perspectives. We will highlight a few workflows including small molecule mass analysis to large intact protein confirmation.
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Presenter: Caroline Chu (LC/MS Application Scientist, Agilent Technologies, Inc.)
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Caroline is an LC/MS Application Scientist with Agilent with over 7 years experience supporting the LC-Q/TOF, QQQ, and IM-QTOF MS and automation, including AssayMap and Rapid Fire. Her focus is on BioPharma applications including intact proteins, native MS, glycomics, high-throughput screening and life science applications including proteomics, targeted proteomics and metabolomics.
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Prior to joining Agilent she completed her undergraduate work at San Francisco State University in Biochemistry before moving on to her Ph.D in Biophysics at the University of California, Davis, working on clinical glycomics with high resolution MS, under the guidance of Professor Carlito Lebrilla. She completed her post-doctoral work at the Institute for Systems Biology in Seattle, WA working targeted proteomics on the human proteome under the guidance of Robert Moritz and in collaboration with Lee Hood and Ruedi Abersold.
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Agilent Technologies: Mass Spectrometry for the Masses, Open Access LC and LC/MS  Using Agilent WalkUp

Mass Spectrometry for the Masses, Open Access LC and LC/MS  Using Agilent WalkUp

Authorities expects from all manufacturers to prevent or reduce the presence of nitrosamines in active pharmaceutical ingredients (APIs) and drug products.
On March 31st, 2021, the EMA guidance for Marketing Authorization Holders will engage a new milestone, called Step 2, and related to the process of products identified to be at risk of N-nitrosamine formation. 
To give you all decision factors on the confirmatory testing, Eurofins BioPharma Product Testing and Waters are glad to invite you to this joined webinar, going through the latest regulatory requirements for testing nitrosamines in solvents, drug substance and drug products.
<ul>
<li>Eurofins’ scientists will provide examples of screening methods performed in their facility located in Toulouse, France.
</li>
<li>Waters will explain how critical it is to combine both LC and MS aspects to quantify traces in highly concentrated matrices.
</li>
</ul>
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Presenter: Elodie Barrau (Site Manager, Eurofins Amatsi Analytics France)
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Elodie Barrau is the Site Manager of Eurofins Amatsi Analytics in Toulouse area, France, with focus in chemistry, manufacturing and control, scientific support (GLP and GMP) for international preclinical, pharma, clinical development and/or registration, and implementation of analytical analysis. Elodie has a degree in Agronomist Engineering specialising in Phytochemistry Drug Development.
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<blockquote>
Presenter: Emmanuel Desmartin (Mass Spec and Bioanalysis Manager, Eurofins Amatsi Analytics France)
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<blockquote>
Emmanuel Desmartin is Mass Spec and Bioanalysis Lab Manager at Eurofins Amatsi Analytics, with 15 years of experience in bioanalysis; holding different positions in CROs as study director in development and validation of LC-MS methods, management of mass spec lab, and technical support.
</blockquote>
<blockquote>
Presenter: Arjan Timmerman (Pharmaceutical Marketing Manager EMEA Pharma, Compliance and Impurities, Waters European Head Quarters)
</blockquote>
<blockquote>
Arjan Timmerman, Experienced Pharmaceutical Marketing Manager with a demonstrated history of working in Regulated Pharmaceutical Industry, is skilled in Life Sciences, Impurities, Compliance and Data Integrity, Method Development and Validation.
</blockquote>

Waters: Nitrosamines Analysis (Step 2): Confirmatory testing

Nitrosamines Analysis (Step 2): Confirmatory testing

Enantiomers are compounds which are non-superimposable mirror image of each other and are challenging to separate without a streamlined method development strategy. Separation, identification and quantification of Chiral isomer ensures safety and efficacy. In addition, food flavors, new pesticide classes are also looked at for enantiomeric separation.
A simple method development strategy will be discussed to optimize chiral separation. Further tips and tricks on solvent switching, Normal phase, reverse phase, polar organic and SFC modes will be discussed on immobilized and coated chiral phases. In addition, scalability aspects from analytical to preparative chromatography will also be explored.
<blockquote>
Presenter: Ramkumar Dhandapani, PhD (Global Product Manager, Phenomenex)
</blockquote>

Phenomenex: Strategies, Tips and Tricks for Simplified Chiral Method Development

Strategies, Tips and Tricks for Simplified Chiral Method Development

Liquid chromatography (LC) is an indispensable method for quality control, characterization, and purification of the nucleic acids. Therapeutic oligonucleotides, mRNA vaccines, and diagnostic oligonucleotide PCR probes are typically analyzed by an ion-pairing reversed-phase LC (IP RP LC) method. Each of these application areas are readily applicable to Covid-19 research.
<h3>In this presentation we’ll discuss:</h3>
<ul>
<li>Principles of IP RP LC</li>
<li>Impact of ion-pairing system choice on oligonucleotide separation selectivity</li>
<li>Purification applications for labeled oligonucleotide probes used in PCR-based diagnostics</li>
<li>Applications of IP RP LC for LC MS for characterization of nucleic acids</li>
</ul>
In addition, we’ll review PREMIER solutions for the acidic nature of nucleic acids that can cause undesirable adsorption on metallic surfaces in LC systems and columns.
<blockquote>
Presenter: Martin Gilar, Ph.D. (Scientific Fellow, Separations Research, Waters Corporation)
</blockquote>
<blockquote>
Dr. Martin Gilar is a Scientific Fellow in Separations Research group at Waters Corporation. He has more than 30 years of experience in the separation sciences, namely chromatography, electrophoresis, and mass spectrometry. His research interest is analysis of biopolymers such as oligonucleotides, peptides, glycans and proteins. He has published over 70 peer reviewed papers.
</blockquote>
<blockquote>
Dr. Gilar’s received his Ph.D. in analytical chemistry from Institute of Chemical Technology in Prague (1996). He spent postdoc years in Hybridon Inc. (1996-1998) and Northeastern University in Boston (1998) developing separation methods for antisense oligonucleotides and fraction collector for DNA molecules. Since 1998 he works at Waters Corp. in Milford, Massachusetts.
</blockquote>

Waters Corporation: A Focus on PCR Diagnostic Probes: Oligonucleotide LC Analysis and Purification

A Focus on PCR Diagnostic Probes: Oligonucleotide LC Analysis and Purification

<h3>Basics and beyond</h3>
A wide range of detection options exist for liquid chromatography, but each has limitations. Charged aerosol detection (CAD) offers an alternative, universal detection option.
<h3>Presentations:</h3>
<h5>Basics and beyond</h5>
<blockquote>
Paul Gamache and Chris Tuczemskyi - Thermo Fisher Scientific
</blockquote>
<h5>Polysorbate analysis in drug products</h5>
<blockquote>
Georg Schuster - Coriolis Pharma
</blockquote>
<h5>CAD in Pharmaceutical Analysis – Selected applications and optimization strategies</h5>
<blockquote>
Ruben Pawellek - Institut für Pharmazie und Lebensmittelchemie University of Wuerzburg 
</blockquote>
<h5>High Throughput Analysis (HTA) for small molecule libraries with the Thermo Scientific Vanquish Duo UHPLC system</h5>
<blockquote>
Thomas Dann and Matthew Gill - Charles River Laboratories
</blockquote>
<h5>Roundtable and Q and A discussion</h5>
<h3>Learning points:</h3>
<ul>
<li>Learn how charged aerosol detection works </li>
<li>What makes charged aerosol detection so powerful </li>
<li>What benefits it offers </li>
<li>What applications are ideal for charged aerosol detection?</li>
</ul>
<h3>Who should attend:</h3>
<ul>
<li>Laboratory managers and Directors</li>
<li>Technology leaders </li>
<li>Principal scientists </li>
<li>Laboratory analyst</li>
</ul>
<blockquote>
Presenter: Chris Tuczemskyi (Applications &amp; Technical Support Specialist, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Chris Tuczemskyi is a chromatography specialist at Thermo Fisher Scientific, based in Cambridge, UK. After spending a number of years building up his experience with chromatography instruments in a water testing laboratory, he joined Thermo Fisher Scientific in 2014 as a chromatography service engineer for Unity Lab Services. Here he developed his passion for supporting customers using HPLC and in 2018 moved into the application specialist team. As a chromatography specialist, his role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Chris graduated from the University of Essex in Biochemistry (Bsc Hons).
</blockquote>
<blockquote>
Presenter: Georg Schuster (Coriolis Pharma)
</blockquote>
<blockquote>
Georg Schuster is senior scientist and group leader at Coriolis Pharma, a science-driven contract research organization, where he focusses on protein characterization and analytic method development for biopharmaceuticals with LC and CE based separation techniques and polysorbate characterization via LC coupled to charged aerosol detection. Georg has received his PhD degree in analytical chemistry from the University of Vienna, Austria, in the field of HILIC stationary phase development and further worked as post-doctoral scientist at the Australian Centre for Research on Separation Science, Tasmania. Prior to working at Coriolis Pharma (2016), he was a scientist engaged in protein separation method development at Novo Nordisk, Denmark.
</blockquote>
<blockquote>
Presenter: Ruben Pawellek (PhD Student, University of Wuerzburg, Germany)
</blockquote>
<blockquote>
Ruben Pawellek received his pharmacy degree at the university of Wuerzburg in 2016, followed by one year of practical training in the quality control unit of Boehringer Ingelheim and in a public pharmacy. Since 2017, he pursues his PhD studies in pharmaceutical chemistry at the university of Wuerzburg. His research is mainly focused on the application of HPLC-CAD in pharmaceutical analysis and on the optimization of the detector’s performance. In 2020, he completed a three-year professional training in pharmaceutical analysis.
</blockquote>
<blockquote>
Presenter: Thomas Dann (Analytical Scientist, Charles River Labs)
</blockquote>
<blockquote>
Graduating from the University of East Anglia with an MChem degree, I went on to complete a PhD there focusing on electroanalytical chemistry, where I synthesised organometallic compounds, characterising them by NMR, mass spectrometry and electrochemical techniques. From this I moved into industry working in a Compound Management lab where the role included QC by LCMS for newly solubilised compounds. Building on this experience, I then progressed onto a full analytical science role at Charles River.
</blockquote>
<blockquote>
Presenter: Matthew Gill (Charles River Labs)
</blockquote>
<blockquote>
Moderator: Frank Steiner (Senior Manager of Product Applications, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Frank Steiner is Senior Manager of Product Applications and coordinates HPLC based scientific collaborations in Thermo Fisher Scientific as scientific advisor. He joined Dionex Corporation in 2005, now a part of Thermo Fisher Scientific, and had been manager in various HPLC marketing functions. Frank received his PhD degree in chemistry in 1995 from Saarland University in Saarbruecken, Germany where he became assistant professor in 1997 and associate professor in 2003, after his postdoc at the nuclear research center in Saclay, France in 1996. Frank published more than 30 publications in refereed journals and more than 10 text book chapters on HPLC topics.
</blockquote>
<blockquote>
Moderator: Paul Gamache (Director Research Development, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Paul is an analytical chemist who joined Thermo Fisher Scientific in 2011 through acquisition of Dionex Corporation. He is a Director of R &amp;D responsible for development of analytical technologies with primary focus on charged aerosol detection (CAD) and electrochemical detection (ECD) for LC. Prior to joining Dionex in 2009, Paul led the development of first and second generation Corona CAD products. He has published more than 50 articles and book chapters and was co-awardee of an NIH Roadmap grant for development of hyphenated EC and LC-MS technologies for metabolomics research. Paul is editor and contributing author to the book “Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques” published in 2017 by John Wiley &amp; Sons, Inc.
</blockquote>

Thermo Scientific: Charged Aerosol Detection Symposium

Charged Aerosol Detection Symposium - Session 1

<h3>Pharma and biopharma applications</h3>
With the flexibility and performance for analytical R&amp;D, and the simplicity and reproducibility needed for manufacturing QA/QC, charged aerosol detection can be used for the analysis of pharmaceuticals (large and small molecule). Learn how CAD can be applied in your work with tips, tricks, pointers, and expert advice from our guest speakers.
<h3>Presentations:</h3>
<h5>Peptide Quantification by CAD</h5>
<blockquote>
Kilian Conde Frieboes - Novo Nordisk 
</blockquote>
<h5>How CAD enabled universal quantification in a high throughput library purification platform</h5>
<blockquote>
Marcel Reck - Novartis Institute for Biomedical Research
</blockquote>
<h5>LC-Charged aerosol detector in early and late-stage pharmaceutical development: Selection of regression models at optimum power function value</h5>
<blockquote>
Imad Haidar Ahmad - Merck Research Laboratories 
</blockquote>
<h5>Roundtable and Q and A discussion</h5>
<blockquote>
With Special guest Ulrike Holzgrabe, Institut für Pharmazie und Lebensmittelchemie University of Wuerzburg 
</blockquote>
<h3>Learning points:</h3>
<ul>
<li>Understand the technology you are using and set it up correctly</li>
<li>CAD is reliable and an easy solution to quantify novel compounds without the need for compound specific calibration </li>
<li>CAD is a key technology in our high throughput library purification platform </li>
<li>Using quantitative LC-CAD in highly regulated laboratories e.g., GLP, GMP</li>
</ul>
<h3>Who should attend:</h3>
<ul>
<li>Laboratory managers and directors </li>
<li>Technology leaders </li>
<li>Principal scientists </li>
<li>Laboratory analyst</li>
</ul>
<blockquote>
Presenter: Kilian Conde-Frieboes (Principal Scientist, Novo Nordisk)
</blockquote>
<blockquote>
Studied Chemistry in Kiel/Germany.Doctor of Science in 1992 (Asymmetric Synthesis)Post docs at the University of Frankfurt and University of California, San Diego (1992-1995).Assistant at the ETH Zurich (1995-1997).Joined Novo Nordisk in 1997.
</blockquote>
<blockquote>
Presenter: Marcel Reck (Research Scientist II, Novartis Institutes for BioMedical Research (NIBR), Switzerland)
</blockquote>
<blockquote>
Before I joined Novartis in 2006, I have previously finished my apprenticeship at the Johannes Gutenberg University in Mainz in 2004 and worked at the Julius Maximilians University of Würzburg. I started my research career at Novartis in Basel as a Scientific Associate supporting Global Discovery Chemistry research projects in the area of Autoimmunity/Transplantation and Inflammation. In 2015, I took an opportunity to focus on analytical and purification research optimizing small molecule separations in the area of analytical and preparative Supercritical Fluid Chromatography and RP HPLC. My organic chemistry expertise has enabled me the opportunity to collaborate more broadly with medicinal chemistry teams and this has recently led to me speer-heading a plate based library initiative focussing on best practice and efficient automated QC, purification and quantification using the CAD detector for MilliLibraries (2-5mg scale) of MedChem research projects.
</blockquote>
<blockquote>
Presenter: Imad Haidar Ahmad, Ph.D (Associate Principal Scientist and supervisor, Merck Rahway, USA)
</blockquote>
<blockquote>
Imad A. Haidar Ahmad received his Ph.D. from Florida State University under the mentorship of Dr. André Striegel. He completed his postdoctoral research with Prof. Peter Carr at University of Minnesota. He is currently an Associate Principal Scientist and supervisor in the Analytical Chemistry Enabling Technology group within MRL’s AR&amp;D department.
</blockquote>
<blockquote>
Moderator: Frank Steiner (Senior Manager of Product Applications, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Frank Steiner is Senior Manager of Product Applications and coordinates HPLC based scientific collaborations in Thermo Fisher Scientific as scientific advisor. He joined Dionex Corporation in 2005, now a part of Thermo Fisher Scientific, and had been manager in various HPLC marketing functions. Frank received his PhD degree in chemistry in 1995 from Saarland University in Saarbruecken, Germany where he became assistant professor in 1997 and associate professor in 2003, after his postdoc at the nuclear research center in Saclay, France in 1996. Frank published more than 30 publications in refereed journals and more than 10 text book chapters on HPLC topics.
</blockquote>
<blockquote>
Moderator: Paul Garnache (Director Research Development, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Paul is an analytical chemist who joined Thermo Fisher Scientific in 2011 through acquisition of Dionex Corporation. He is a Director of R &amp;D responsible for development of analytical technologies with primary focus on charged aerosol detection (CAD) and electrochemical detection (ECD) for LC. Prior to joining Dionex in 2009, Paul led the development of first and second generation Corona CAD products. He has published more than 50 articles and book chapters and was co-awardee of an NIH Roadmap grant for development of hyphenated EC and LC-MS technologies for metabolomics research. Paul is editor and contributing author to the book “Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques” published in 2017 by John Wiley &amp; Sons, Inc.
</blockquote>
<blockquote>
Presenter: Prof. Dr. Ulrike Holzgrabe (University of Würzburg)
</blockquote>
<blockquote>
Prof. Dr. Ulrike Holzgrabereceived her diploma degree in Chemistry at the University of Marburg in 1979, her approbation in Pharmacy in 1982, and her PhD in Pharmaceutical Chemistry in 1983 both at the University of Kiel. After habilitation in Pharmaceutical Chemistry in Kiel 1989 she became an associate professor at the University of Bonn (1990-1999). In 1988, she was a post-doc fellow at the University of Bath, UK, with A. Casy, in 1994 a visiting professor at the University of Erlangen with J. Gasteiger, and 1995 at the University of Illinois at Chicago with A.F. Hopfinger. Since 1999 she holds a full professorship at the University of Würzburg (declining offers from the universities of Tübingen, Münster and Berlin, and the CEO position of the BfArM). She served as vice-rector of the University of Bonn from 1997-1999 and as a vice-president of the University Wuerzburg from 2018-2021. She was president of the German Pharmaceutical Society from 2004 till 2007. She is/was a member of the German and European Committee of the Pharmacopoeia (BfArM, EDQM), and a member of the scientific boards of the BfArM, BPS of FIP, and the HIPS as well as a collegiate of the DFG. In 2020, she received the “Bayerischen Verdienstorden” (Bavarian order of merit). Her research interests are the development of antiinfectives and GPCR ligands as well as quality analysis of drugs.
</blockquote>
<blockquote>
She is co-owner of Aamuthera, a company dealing with the development of anticancer drugs.
</blockquote>

Charged Aerosol Detection Symposium - Session 2

<h3>Food and beverage applications</h3>
Did you know that CAD can be used in application areas such as food and beverages, in lipid analysis for food fraud and for sugar fingerprinting? It’s also used to detect specialty chemicals and polymers, such the environmentally toxic agent polyacrylic acid.
<h3>Presentations:</h3>
<h5>CAD Tips and Tricks</h5>
<blockquote>
Paul Gamache and Chris Tuczemskyi - Thermo Fisher Scientific
</blockquote>
<h5>A UHPLC-PDA-CAD-HRMS Platform for Comprehensive Identification and Quantitation of Constituents in Complex Mixtures for Safety Risk Assessments</h5>
<blockquote>
Dr. Jason Price - Procter &amp; Gamble R&amp;D 
</blockquote>
<h5>Using UHPLC-CAD and PCA to detect olive oil adulteration via triacylglycerol analysis</h5>
<blockquote>
Hilary Green - UC Davies
</blockquote>
<h5>Roundtable and Q and A discussion</h5>
<h3>Learning points:</h3>
<ul>
<li>How food adulteration can be quickly detected</li>
<li>Predict the level of adulteration and the adulterant</li>
<li>Quantitation for analytes where analytical reference standards are not available</li>
<li>Using CAD with other detectors</li>
</ul>
<h3>Who should attend:</h3>
<ul>
<li>Laboratory managers and directors </li>
<li>Technology leaders </li>
<li>Principal scientists </li>
<li>Laboratory analyst</li>
</ul>
<blockquote>
Presenter: Jason M. Price (Principal Scientist, Procter &amp; Gamble, USA)
</blockquote>
<blockquote>
Jason Price received his Ph.D. in Chemistry from Purdue University in 2002 where he worked with Professor Hilkka Kenttämaa to understand the factors that influence the fundamental reactivity of organic radicals and biradicals via their ion-molecule reactions in Fourier transform ion cyclotron resonance mass spectrometers. After graduation, Jason joined Procter &amp; Gamble in Cincinnati, OH working in Beauty Analytical to drive upstream technologies to in-market products across Personal Cleansing, Skin Care and Hair Care categories. He developed expertise in quantifying deposition and bioavailability of actives to guide formulation development and claim support. Jason is currently a Principal Scientist in P&amp;G’s Trace Analytical Capability where he has worked since 2009. In this role, he supports all P&amp;G businesses by leading (1) the development of quantitative LC-MS/MS assays and (2) the identification of unknowns (e.g., impurities, degradation products, contaminants) in complex mixtures by high-resolution mass spectrometry.
</blockquote>
<blockquote>
Presenter: Hilary Green (Graduate student, Ph.D. Candidate, University of California, Davis USA)
</blockquote>
<blockquote>
Hilary’s is a Ph.D. candidate in the Agricultural and Environmental Chemistry group at the University of California, Davis. Her interest in research interests stem from her passions for food and the environment through the lens of analytical chemistry. Her dissertation focuses on ensuring the authenticity and quality of edible oils. She has published a faster and less wasteful method to detect olive oil adulteration. She also published a work evaluating the chemical composition of avocado oils on the US market. For the final part of her dissertation, she is working to understand the natural variance in avocado oils to support the standard development effort for this oil. In addition, she is developing a better method to detect adulteration in avocado oil. Hilary’s research is closely connected to the food industry and consumers; thus, she is broadly interested in educating the public on scientific research.
</blockquote>
<blockquote>
Presenter: Ulrike Holzgrabe (Univ of Wuerzburg)
</blockquote>
<blockquote>
Moderator: Frank Steiner (Senior Manager of Product Applications, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Frank Steiner is Senior Manager of Product Applications and coordinates HPLC based scientific collaborations in Thermo Fisher Scientific as scientific advisor. He joined Dionex Corporation in 2005, now a part of Thermo Fisher Scientific, and had been manager in various HPLC marketing functions. Frank received his PhD degree in chemistry in 1995 from Saarland University in Saarbruecken, Germany where he became assistant professor in 1997 and associate professor in 2003, after his postdoc at the nuclear research center in Saclay, France in 1996. Frank published more than 30 publications in refereed journals and more than 10 text book chapters on HPLC topics.
</blockquote>
<blockquote>
Moderator: Paul Gamache (Director Research Development, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Paul is an analytical chemist who joined Thermo Fisher Scientific in 2011 through acquisition of Dionex Corporation. He is a Director of R &amp;D responsible for development of analytical technologies with primary focus on charged aerosol detection (CAD) and electrochemical detection (ECD) for LC. Prior to joining Dionex in 2009, Paul led the development of first and second generation Corona CAD products. He has published more than 50 articles and book chapters and was co-awardee of an NIH Roadmap grant for development of hyphenated EC and LC-MS technologies for metabolomics research. Paul is editor and contributing author to the book “Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques” published in 2017 by John Wiley &amp; Sons, Inc.
</blockquote>

LCMS webinars focusing on HPLC - page 65