Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"><h4>The Role of Chromatography in Forensic Food Analysis</h4>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:<ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate adulteration.</li></ul>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.<h4>Isocratic vs. Gradient Elution</h4>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:<ul><li>Isocratic HPLC uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li>Gradient HPLC changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.<h4>Watrex Praha and the Contribution of Modern Systems to Food Analysis</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha, s.r.o.</a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline">Streamline™ product line</a> includes several HPLC system concepts suitable for food analysis:<ul><li>Isocratic HPLC system – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li>Binary gradient HPLC system – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures (e.g., phenolic profiles of wine, components of aromatic extracts).</li><li>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography) – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf">Product catalogue HERE</a></blockquote>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.<h4>Practical Examples of Adulteration Revealed by HPLC</h4><ul><li>Detection of olive oil dilution: HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li>Estimation of sugar addition in honey: Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li>Identification of aromatic adulterants in wine: Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4>Conclusion</h4>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.<blockquote>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true">LinkedIn</a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague">Facebook</a>) for more engaging topics.</blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.<h4>In this episode, you'll learn:</h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.<h4>Referenced Papers:</h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.<h3>Video Transcription</h3><h4>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</h4><h5>Introduction</h5>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.<h5>Research Focus: Malaria Drug Discovery and Kinase Targets</h5>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.<h5>Chromatography in Protein Expression and Purification Workflows</h5>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.<h5>Integrating Size Exclusion Chromatography into the Workflow</h5>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.<h5>The Role of Mass Spectrometry in Biomolecular Characterization</h5>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.These approaches are essential for understanding how candidate drug targets function within complex biological systems.<h5>Sample Preparation Challenges in Biological Research</h5>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.<h5>Advances in Chromatography and MS for Malaria Research</h5>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.<h5>Malaria, Drug Resistance, and Global Impact</h5>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.<h5>Advice for Early-Career Researchers</h5>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.<h5>Closing Remarks</h5>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

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The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

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Agilent Technologies Molecular Instrumentation and Applications...
<blockquote>
Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)
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<blockquote>
Mark, an Application Scientist for Agilent Technologies, has over 30 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.
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Demystifying Molecular Instrumentation and Applications

Agilent Technologies Molecular Instrumentation and Applications...

This webinar will guide you on the best practices, tools, and techniques needed to get the most reliable, verifiable insights into your optical components. Appropriate choice of technique and methodology is critical to getting the information you need to improve design, verify product performance, and accelerate manufacturing.
We will explore the subtleties of reflection and transmission measurements at normal incidence and at oblique angles, emphasizing high absorbance, optical density, linearity, and effects of incident beam collimation. We will discuss diffuse scattering measurements and automation/robotics to broaden quality assurance programs and speed up analysis. 
The webinar is relevant to developers of optics, photonics, and electro-optical or semiconductor devices such as visual displays, laser-based range detection (LiDAR), optical communications systems and light-based scientific instrumentation. Improve your understanding of the link between your desired outcomes and the spectrophotometric approaches needed to achieve those insights. 
Key Learning Objectives:
<ul>
<li>The best spectrophotometric practices to extract deeper insights into the characteristics of optical samples.</li>
<li>How to overcome the limitations of traditional spectrophotometric tools and verify outcomes.</li>
<li>Accelerating QA/QC of optics in a production environment.</li>
</ul>
Who Should Attend:
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<li>Commercial or Academic organizations in the fields of optics, photonics, electro-optical, or semiconductors</li>
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Presenter: Travis Burt (Global Product Manager – Cary UV-Vis-NIR Spectrophotometers, Agilent Technologies)
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Presenter: Catherine Dold (Health &amp; Environment Writer, C&amp;EN Media Group)
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C&EN: Optimizing Optics Using Advanced Spectrophotometry

Optimizing Optics Using Advanced Spectrophotometry

This webinar will guide you on the best practices, tools, and techniques needed to get the most reliable, verifiable insights into your optical components.

There are many different applications which can be run on an UV-Vis-nir instrument. Within these applications there are a number of instrument parameters which the user can adjust to improve the quality of the data collected or use to optimize the collection time.
A discussion will be held around both available applications and pertinent instrument parameters.
<blockquote>
Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark, an Application Scientist for Agilent Technologies, has over 30 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.
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Agilent Technologies: Navigating Your Way through Parameters and Applications of an UV-Vis-nir Instrument

Navigating Your Way through Parameters and Applications of an UV-Vis-nir Instrument

A discussion will be held around both available applications and pertinent instrument parameters.

Parameters and Applications of an UV-Vis-nir Instrument

Agilent&#39;s Cary 3500 Flexible UV-VIS Spectrometer makes concentration analysis easy. Its unique hardware design allows you to effortlessly change cuvette pathlengths for switching between assays.
In this presentation, we will discuss concentration analysis workflows and how to streamline these measurements with reliable hardware and software.
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Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)
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Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.
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Agilent Technologies: Simple UV-VIS Workflows For Concentration Analysis

Simple UV-VIS Workflows For Concentration Analysis

We will discuss concentration analysis workflows and how to streamline these measurements with reliable hardware and software.

Agilent&#39;s Cary 3500 Flexible UV-VIS Spectrometer provides the accuracy and reliability needed for meeting compliance standards.
This presentation will discuss an overview of the instrument&#39;s newest functions as well as the software&#39;s ability to maintain data integrity through Agilent&#39;s OpenLab Platform.
<blockquote>
Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.
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Agilent Technologies: Meeting 21 CFR 11 Compliance with the Cary 3500 Flexible UV-VIS Spectrometer

Meeting 21 CFR 11 Compliance with the Cary 3500 Flexible UV-VIS Spectrometer

We will discuss an overview of the instrument's newest functions as well as the software's ability.

Meeting 21 CFR 11 Compliance with the Cary 3500 UV-VIS

Have you fully exploited the potential of your analytical techniques in edible oil analysis? Our experts will reveal what you can achieve with CNSX-analysis, ICP-OES, AAS and UV/Vis in regulated standard analysis and beyond.
Price fluctuations, supply bottlenecks and export stops have highlighted in recent years how volatile and complex the supply chains of edible oils are. Exporters, importers and processors of edible oils face the challenges of rapid supplier qualification while achieving regulatory compliant, seamless, and efficient quality monitoring of their crude oil, intermediates and final products.
As agricultural products, edible oils are subject to natural quality fluctuations, while cultivation methods and long transport routes pose risks of contamination and oxidation. In addition, they go through a temperature- and additive-intensive process with the refinery, during which undesirable by-products can arise under certain circumstances.
Our webinar is aimed at industry, regulatory and contract laboratories involved in the analysis of edible oils. With combustion elemental analysis, ICP-OES, AAS and UV/Vis molecular spectroscopy, our experts will introduce four techniques which enable precise, efficient, and standard-compliant quality and process analysis of edible oils. They will also show you tips and tricks on how to exploit the additional potential for optimising your analytical workflows regarding the targeted and economic use of elaborate specific techniques.
Learn how to use these techniques for a variety of oils at different steps in the value and processing chain, and how to achieve optimal results and increase sample throughput.
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<ul>
<li>Explore how to carry out the comprehensive analysis of toxic elements, process inhibitors, catalyst poisons as well as the macro-mineral content with ICP-OES.
</li>
<li>Learn what combustion analysis can achieve in quality control and process monitoring, especially regarding the formation of unwanted compounds during refining.
</li>
<li>Discover how to streamline analytical workflows with quick screens for organic and inorganic Sulfur and Nitrogen as sum parameters with combustion analysis.
</li>
<li>Find out how to assess oxidation in oils via DOBI index, and how to monitor the efficiency of bleaching in refinery using UV/Vis spectrometry.
</li>
<li>Discover what AAS can do as an alternative spectrometric element technique in the analysis of edible oils.
</li>
<li>Meet our experts and discuss with us in the Q&amp;A.
</li>
</ul>
<blockquote>
Presenter: Dr. Angela Gröbel (Senior Product Manager / Expert Supervisor Chemical Analysis)
</blockquote>
<blockquote>
Angela is a Senior Product Manager and Expert Supervisor for the combustion elemental analysis and related sample preparation portfolio. She holds a PhD in analytical chemistry. Angela developed Analytik Jena’s first trace elemental analysis product line for organic matter, later she became an expert in determination of Sulfur, Nitrogen, and Chlorine across a broad range of industry segments, including oil and gas, food and agriculture and environmental protection. Since 2013 Angela has been Product Manager, responsible for the entire combustion elemental analysis and related sample preparation portfolio.
</blockquote>
<blockquote>
Presenter: Dr. Holger Sievers (Application Specialist Chemical Analysis)
</blockquote>
<blockquote>
Holger Sievers is an Application Specialist at Analytik Jena. He holds a PhD from the Technical University of Munich in the field of ultra-trace analysis of noble metals which he obtained after his Master’s focusing on inorganic and analytical chemistry. Holger joined Analytik Jena in 2018 after seven years of teaching at the University and is currently engaged in chemical analysis. His primary focus lies in atomic absorption spectrometry and element analysis in general.
</blockquote>
<blockquote>
Presenter: Sandra Wunsch (Application Specialist Chemical Analysis)
</blockquote>
<blockquote>
Sandra Wunsch has been an Application Specialist for molecular spectrometry at Analytik Jena since 2016. She has intense in-depth knowledge in scientific and industrial application of molecular spectrometry, and extensive experience in the market and application fields, especially in the areas of environment and food &amp; agriculture. As a graduated Nutritionist, she is an expert in the use of chemical analysis and especially the determination of molecular compounds in the food industry and related fields.
</blockquote>

New Food: Edible oil processing: How to monitor elements and compounds

Edible oil processing: How to monitor elements and compounds

How to use these techniques for a variety of oils at different steps in the value and processing chain, and how to achieve optimal results.

While making a UV-Vis measurement is simple, making an accurate measurement requires an understanding of instrument parameters and their effects on the measurement. Parameters include Baseline correction, spectral bandwidth, data interval, and many additional settings.
This presentation will provide an insight into these parameters and more.
<blockquote>
Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark, an Application Scientist for Agilent Technologies, has over 35 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.
</blockquote>
<blockquote>
Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.
</blockquote>

Agilent Technologies: Understanding UV-Vis Instrumental Parameters

Understanding UV-Vis Instrumental Parameters

This presentation will provide an insight into baseline correction, spectral bandwidth, data interval, and many additional settings

Examining powdered samples in the presence of a reactive gas or at elevated temperatures could be a difficult measurement to make.
During this workshop, we will be looking at how to make such diffuse reflection measurements using a high-end Cary instrument and an accessory from Harrick Scientific known as the Praying Mantis. Tips and tricks of using this accessory will also be discussed.
<blockquote>
Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark, an Application Scientist for Agilent Technologies, has over 35 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.
</blockquote>
<blockquote>
Presenter: Sue Berets, PhD (Applications Scientist, Harrick Scientific Products)
</blockquote>
<blockquote>
Sue, an Applications Chemist for Harrick Scientific Products, has over 30 years of experience in accessories and optics for molecular spectroscopy. Sue received her doctorate in physical chemistry from Tufts University and has carried out basic research in theoretical and applied spectroscopy. Her responsibilities include technical and sales support, including accessing products for specific customer applications. She is involved in new product development, from initial concept to finished product, to meet the every changing applications needs of the market.
</blockquote>

Agilent Technologies: Unique Measurement Capabilities Using the Praying Mantis and a Cary UV-Vis system

Unique Measurement Capabilities Using the Praying Mantis and a Cary UV-Vis system

How to make such diffuse reflection measurements using a high-end Cary instrument and an accessory from Harrick Scientific known as the Praying Mantis.

Using the Praying Mantis and a Cary UV-Vis system

UV-Vis spectrophotometric analysis is vital for Pharma and Biopharma labs, advancing drug development, quality control, and research.
Explore how the Agilent Cary 3500, in synergy with OpenLab Software, conquers challenges, ensures data integrity, and maximizes productivity. Its modular design tailored to your application needs streamlines complex measurements with minimal sample preparation. With an advanced xenon flash lamp offering a 10-year replacement warranty and no warm-up time, rest assured your sensitive samples are safeguarded for reliable and cost-effective analysis.
Learning Objectives:
<ol>
<li>Overcoming Challenges with Long Pathlength Analysis
</li>
<li>Latest Technology for Solid Sample Analysis
</li>
<li>Simultaneous Measurements with Temperature Control using Multicell
</li>
<li>Simplifying Complex Measurements for Protein and Biological Samples
</li>
<li>Enhancing Laboratory Productivity, Cost Saving, and Ease of Use
</li>
<li>Achieving Data Integrity and Compliance with Global Regulations
</li>
</ol>
Who should attend?
<ul>
<li>Pharma &amp; Biopharma professionals
</li>
<li>QA/QC laboratory analysts and managers
</li>
<li>Researchers, academicians and students in Life Sciences
</li>
</ul>
<blockquote>
Presenter: Shubham Sengupta (Product Specialist – Spectroscopy, Agilent Technologies)
</blockquote>
<blockquote>
Shubham Sengupta is a Spectroscopy Product Specialist at Agilent Technologies, with 14 years of diverse experience ranging from CRO to CTL. He holds a postgraduate degree in Chemistry from Indian School of Mines, Dhanbad (IIT-Dhanbad).
</blockquote>

Agilent Technologies: Powering UV-Vis Analysis in Pharma & Biopharma: Agilent Cary 3500

Powering UV-Vis Analysis in Pharma & Biopharma: Agilent Cary 3500

Overcoming Challenges with Long Pathlength Analysis.

Powering UV-Vis Analysis in Pharma & Biopharma

<h5>The Problem:</h5>Plasmid DNA (pDNA) is more popular than ever as a critical starting material for viral vectors and mRNA-LNPs, but its high cost means analytics need to deliver data from almost no sample. Knowing purity and total concentration is crucial when producing or working with pDNA, so you always need to monitor how much you have and when impurities are lurking. When samples aren’t pure pDNA, only looking at A260/230 and A260/280 ratios via spectrophotometry won’t help you find out the amount of plasmids you have and what else is really there.<h5>The Solution:</h5>Lunatic only needs 2 µL to quant and QC your pDNA, delivering spot-on results for up to 96 samples in just 10 minutes without any expensive dyes or standards. Besides QC’ing a boatload of plasmid preps, Lunatic also overcomes common hurdles to deliver results right on the money even when contaminants are present.<h5>The Proof:</h5>During this virtual seminar you’ll learn what Lunatic is all about and see how it quantifies plasmid DNA samples fast, accurately, and with just 2 µL of sample, no dilutions needed. We’ll check out how Lunatic’s lightning fast UV/Vis measurements spot any dirt in your samples, and take a sneak peek at other great tools to drive pDNA manufacturing success.<blockquote>Presenter:&nbsp;Kevin Lance PhD (Marketing Manager, Analytical Instruments, Unchained Labs)Presenter:&nbsp;Joanna Winkler (Application Scientist, Viral Vectors, Marketing Department, Unchained Labs)</blockquote>

Unchained Labs: Get a grip on your pDNA characterization with Lunatic

Get a grip on your pDNA characterization with Lunatic

You’ll learn what Lunatic is all about and see how it quantifies plasmid DNA samples fast, accurately, and with just 2 µL of sample

LCMS webinars focusing on UV-Vis - page 7