<h3>A Dedicated Solution for Oligonucleotide Analysis</h3>Oligonucleotides have become key players in modern pharmaceutical and biological research, particularly in the development of highly targeted and personalized therapies. As increasing numbers of oligonucleotide-based drugs enter clinical trials or receive regulatory approval, analytical laboratories face growing demands for both throughput and data quality. These molecules present unique chromatographic challenges due to their long, highly polar structures and subtle variations in nucleobase sequence or functionalization.<h3>Sepapure oliGO – Designed for Oligonucleotide Separations</h3>Selecting the optimal chromatographic approach for oligonucleotide analysis can be complex. Many laboratories rely on ion-pair reversed-phase (IP-RP) chromatography because of its speed, robustness, and compatibility with mass spectrometry. However, commonly used ion-pair reagents can degrade conventional silica-based stationary phases.<a target="_blank" rel="noopener noreferrer" href="https://www.knauer.net/columns-oligonucleotide-columns">Sepapure oliGO</a> addresses this limitation through a hybrid silica matrix combined with specialized surface end-capping, ensuring resistance to aggressive IP-RP reagents. Its functionalized surface enables both strong reversed-phase interactions and controlled ionic contributions, effectively creating a mixed-mode separation mechanism. This design results in improved durability compared with standard mixed-mode columns, making Sepapure oliGO a robust choice for routine oligonucleotide workflows.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Sepapure_oli_GO_columns_with_laser_etched_column_information_23f545be81.jpg" alt="KNAUER: Figure 1 - Sepapure oliGO columns with laser-etched column information." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Sepapure_oli_GO_columns_with_laser_etched_column_information_23f545be81.jpg 223w," sizes="100vw" width="1186" height="828"><h3>Method Transfer and Comparative Performance</h3>In collaboration with BianoGMP GmbH, KNAUER’s application team evaluated Sepapure oliGO against a widely used reference column. The study compared oligonucleotides of varying lengths and purities, including a fully thiolated 43-mer DNA. Particular attention was paid to potential interactions between thiolated oligonucleotides and stainless-steel hardware, which have been reported to cause peak tailing.The results demonstrated that direct method transfer between the reference column and Sepapure oliGO was generally feasible without significant adjustment. Chromatographic resolution remained comparable, while retention times were slightly shorter on Sepapure oliGO. Furthermore, the anticipated benefits of expensive bioinert hardware were not confirmed under the tested conditions, suggesting that such effects may primarily influence very fast (U)HPLC separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Overlayed_chromatogram_of_crude_product_of_a_43mer_thiolate_oligonucleotide_7ae9613efd.jpg" alt="KNAUER: Figure 2: Overlayed chromatogram of crude product of a 43mer thiolate oligonucleotide. Light blue - reference column and dark blue – Sepapure oliGO." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Overlayed_chromatogram_of_crude_product_of_a_43mer_thiolate_oligonucleotide_7ae9613efd.jpg 245w," sizes="100vw" width="1223" height="453"><h3>Low Backpressure for Reliable Operation</h3>Sepapure oliGO can be applied effectively in both HPLC and (U)HPLC workflows. Its low operating backpressure minimizes risks associated with clogging, excessive system wear, and performance deterioration over time. Typical IP-RP separations using 3 µm particles generated pressures around 200 bar, enabling laboratories without ultra-high-pressure systems to perform high-quality oligonucleotide analysis.<h3>Best Practices for Column Performance</h3>To maintain optimal performance, users should observe recommended operating conditions, including a broad pH range of 1.5–10 and temperatures up to 60 °C. Employing a guard column can prevent coarse synthesis by-products from reaching the analytical column. If pressure increases are observed, backflushing at low flow rates can restore performance.When the system is idle, thorough flushing to remove salts is essential. For storage, the column should be kept in a 1:1 mixture of methanol–water or acetonitrile–water. Gradient transitions are recommended when switching between standard RP eluents and IP-RP reagents. Regeneration procedures developed for RP columns are also suitable for Sepapure oliGO.<h3>Beyond Analytical Separations</h3>For large-scale purification, ion-exchange chromatography (AEX) may offer advantages. Scalable preparative systems are available to support purification workflows, ranging from compact laboratory setups to pilot-scale platforms with flow rates up to 800 mL/min. Bioinert configurations can be integrated where required, allowing laboratories to tailor purification solutions to their specific oligonucleotide applications.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at sales@knauer.net. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at folmert@knauer.net&nbsp;</li></ul></blockquote>

Oligos Made Easy - Part 8: Sepapure oliGO

Visit the LabRulez booth at <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/871">analytica 2026</a> in Munich, 24. - 27. March 2026! You can find us in Hall A1, Booth A1.506. At the booth, you will be able to explore live demonstrations of how the LabRulez portals work and see selected analytical instruments from our pre-owned marketplace section.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg" alt="LabRulez at Analytica 2026: Hall A1 Floor Plan and Booth Location" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg 245w," sizes="100vw" width="1516" height="881"><h3>Would you like to learn how the LabRulez portals work?</h3>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/">LabRulezLCMS</a> ➡︎ <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/">LabRulezGCMS</a> ➡︎ <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/">LabRulezICPMS</a><ul><li>A unique online solution for your marketing</li><li>A targeted audience from the analytical chemistry community</li><li>Modern technologies and a unique concept</li><li>Design optimized for most smart devices – PC, mobile, and tablet</li><li>Advanced statistical tools fully available to users</li></ul><h3>Services Offered</h3><h4>News + Company Updates</h4><ul><li>Publish news related to your instrumentation, applications, services, events, job openings, and more</li><li>News is shared both on our portals and across our social media channels</li></ul><h4>Library – All Documents in One Place</h4><ul><li>Applications, posters, presentations, technical articles, brochures, specifications</li><li>Fast and advanced search capabilities</li><li>Over 32,000 documents currently available in our database</li></ul><h4>Webinars</h4><ul><li>Upcoming and on-demand webinars from the field of analytical chemistry</li><li>More than 5,500 webinars covering chromatography, mass spectrometry, and spectroscopy</li></ul><h4>Careers</h4><ul><li>Targeted job listings in analytical chemistry</li><li>Clear filtering by specialization or location</li><li>Increased visibility for job postings</li></ul><h4>Companies</h4><ul><li>Company profile with key information and contacts</li><li>Integration of all your activities on our portal: News, Library documents, Webinars, Upcoming Events, Careers, and mentions by partners or customers</li><li>Connection to your Products listed on LabRulez portals</li></ul><h4>Products and Services</h4><ul><li>Comprehensive overview of instrumental techniques and services</li><li>Targeting the analytical chemistry community and customers in the growing online environment</li></ul><h4>Marketplace</h4><ul><li>Buying and selling pre-owned analytical equipment</li><li>We purchase analytical instrumentation of all brands and ages</li><li>Sell your equipment through LabRulez</li><li>Request and purchase complete systems, modules, or spare parts with warranty and service</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg" alt="LabRulez at analytica 2026" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg 88w," sizes="100vw" width="1600" height="2844"><h3>What Will Be on Display at Our Booth?</h3>Come and explore selected spare parts and modules from our marketplace section. These include both new, tested, and pre-owned items.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar">LabRulezLCMS Marketplace</a></h4>Spare parts for LC/MS:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=378">Vacuum turbo pumps for LC/MS</a></li></ul>Spare parts for HPLC:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=397">Degassers for HPLC/UHPLC</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=368&amp;search=valve">Automatic valves for HPLC</a></li><li>HPLC components for Agilent 1100, 1200, and 1260 Infinity II series</li></ul>Consumables:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=377">HPLC lamps</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=48">HPLC columns</a></li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar">LabRulezGCMS Marketplace</a></h4>Spare parts for GC/MS:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=367&amp;search=turbo">Turbo pumps for GC/MS</a></li></ul>Spare parts for GC:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=366&amp;search=EPC">EPC modules</a></li></ul>Sample injection<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar/432">Autosampler 7693A for GC</a></li></ul>Consumables:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=7">GC columns </a>and selected accessories</li></ul>

LabRulez at analytica 2026

News from LabRulezLCMS Library - Week 13, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Technology Networks

Shimadzu Corporation

MALDI-MS is a fast, label-free technology for assays in pharmaceutical R&amp;D and chemical biology. Recently, CeMOS has pioneered MALDI-MS based cell assays for drug profiling and screening, in part in a collaboration with a pharma company.
The first part of the webinar will present examples of MALDI-MS phenotypic and mechanistic cell assays. It will cover the discovery of potential cellular drug response markers, characterization of potent fatty acid synthase inhibitors. 
The second part will introduce the use of MALDI-MS instead of radioisotope- or fluorescence-based methods for investigation of cellular drug uptake, assay automation and evaluation of drug-drug interactions mediated by the transporter OATP2B1. Characterization of this transporter, screening of 300 drugs for inhibition of uptake and IC50 calculation for hit compounds will be presented.
<h3>Key Learning Objectives:</h3>
<ul>
<li>MALDI MS cell-based assays are a great label-free analytical tool for the investigation of transport processes</li>
<li>Cell- based screening of inhibitors can be achieved by using a pipetting robot combined with the MPP software on the rapifleX</li>
<li>The identification of substrates and especially inhibitors of transport proteins can reveal possible drug-drug interactions</li>
<li>MALDI MS cell assays can help to identify drug response markers, aid compound profiling, and enable future cell-based screening</li>
</ul>
<h3>Who Should Attend:</h3>
<ul>
<li>Assay development and screening experts in pharmaceutical R&amp;D</li>
<li>Drug safety experts interested in in-vitro profiling of drug-drug interactions</li>
<li>Chemical biologists interested in other, label-free possibilities for analysis of compound action in cells</li>
<li>Mass spectrometry experts with a keen interest in innovative applications of MALDI MS</li>
</ul>
<blockquote>
Presenter: Prof. Dr. Carsten Hopf (Professor and Head of CeMOS)
</blockquote>
<blockquote>
Carsten Hopf is currently professor of bioanalytics and drug discovery at Mannheim Technical University. A trained neurochemist, Carsten obtained his PhD in biochemistry from University of Tübingen in collaboration with the Max-Planck-Institute for Developmental Biology. As an EMBO fellow, he continued his research at the Johns Hopkins University School of Medicine, in Baltimore, USA. From 2001 to 2014, he worked at Cellzome (since 2012 part of GlaxoSmithKline), a leading proteomics-based drug discovery company, eventually serving in its leadership team. Since 2005, he is a professor of bioanalytics, enjoying the best of both worlds by building bridges between academia and industry. Carsten heads the Center for Mass Spectrometry and Optical Spectroscopy (CeMOS) in Mannheim. He also serves as spokesman for the Pharma/Diagnostics public-private partnership for innovation M2Aind and is the industry liaison for the German Society of Biochemistry and Molecular Biology.
</blockquote>
<blockquote>
Presenter: Melissa Unger, M.Sc (Scientist at CeMOS)
</blockquote>
<blockquote>
Melissa Unger received a B.Sc. and M.Sc. in biology from Würzburg University, specializing in Human Genetics and Pharmaceutical Biology. Since 2017 she has been a PhD student at CeMOS, and she has just submitted her PhD thesis to Heidelberg University. She has received the Tony B. Award of the Society for Laboratory Automation and Screening (SLAS) for her work on MALDI-MS assays of drug uptake and drug-drug interactions.
</blockquote>

Technology Networks: MALDI-MS-based assays for cellular drug action and transport with rapifleX MPP

MALDI-MS-based assays for cellular drug action and transport with rapifleX MPP

We will also offer a practical example, demonstrating how high spatial resolution MALDI-imaging is being used to identify previously unobserved changes in the lipidome of human colon in the context of inflammatory bowel disease, which provides new insights into the mechanisms of this disease.
<blockquote>
Presenter: José A. Fernández (Professor, Universidad del País Vasco)
</blockquote>
<blockquote>
Prof. José A. Fernández obtained his Ph. D. in physical chemistry in 1995 in the University of the Basque Country (UPV/EHU), with a study on reactions of carbene radicals in gas-phase. Then, he moved to Colorado State University, where he worked in the characterization of pre-reactive aggregates using LIF and REMPI techniques. In 2001, he returned to the UPV/EHU, thanks to the prestigious Ramon y Cajal program, created to bring back to the Spanish system of Science and Technology outstanding researchers. Upon incorporation to the UPV/EHU, he started a research line in laser spectroscopy of molecular aggregates in jets, which he latter complemented with experiments in ZEKE-PFI and MW spectroscopy. In 2005 he started a second research line in MALDI-Imaging of lipids. Since then, Prof. Fernandez’s group has produced some important results in this field, and has stablished collaboration with multiple institutions in several countries. He is also president of the Spanish division of Atomic and Molecular Physics.
</blockquote>
<blockquote>
Presenter: Nazim Boutaghou (Life Science Product Manager, Shimadzu Scientific Instruments)
</blockquote>
<blockquote>
Nazim Boutaghou is the Life Science Product Manager at Shimadzu Scientific Instruments (SSI). He oversees SSI’s wide portfolio of MALDI instrumentation, including the newly released MALDI Digital Ion Trap. Nazim Boutaghou graduated from a Master in Chemistry with a minor in Biology from the University of Bordeaux 1 (Bordeaux, France) continued with a Master in Business Management from the Bordeaux Institute of Business Administration (IAE). He moved to the US in 2006 to further his knowledge in Analytical Chemistry and Mass Spectrometry, where he joined Dr. Richard Cole’s group at the University of New Orleans. He graduated from his PhD in 2011 and joined Shimadzu in 2013 as an Applications Scientist specialized in MALDI-based analyses.
</blockquote>

Shimadzu ASMS 2020: Mapping Uncharted Territory: MALDI-imaging of Lipids in Tissues 

LCMS webinars focusing on MALDI - page 8

MALDI-MS-based assays for cellular drug action and transport with rapifleX MPP

Mapping Uncharted Territory: MALDI-imaging of Lipids in Tissues

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