Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

High-performance liquid chromatography (HPLC) remains one of the most widely used analytical techniques for separating, identifying, and quantifying compounds in complex mixtures. Successful HPLC method development depends not only on selecting the correct column and optimizing the mobile phase composition, but also on choosing the appropriate detector. Even a perfectly optimized chromatographic separation provides little value if the detection system cannot properly capture and interpret the analytes of interest.In many ways, the detector acts as the “vision system” of the chromatograph. It converts separated compounds into measurable electronic signals, allowing analysts to identify components and determine their concentrations. The choice of detector therefore has a major impact on sensitivity, selectivity, robustness, and the overall quality of analytical data.This article provides an overview of the most common HPLC detection techniques, discusses their strengths and limitations, and outlines the most important criteria to consider when selecting a detector for a specific analytical task.<h3>HPLC Detection Technologies</h3>HPLC detectors are typically divided into two major categories:<ol><li>Specific detectors (solute-property detectors)</li><li>Bulk property detectors</li></ol>Specific detectors respond to a particular chemical or physical property of the analyte. Ideally, their signal is largely unaffected by changes in mobile phase composition. Common examples include UV/Vis detectors, fluorescence detectors, and mass spectrometers.Bulk property detectors, on the other hand, measure differences between the pure mobile phase and the mobile phase containing analytes. These detectors are considered more universal because they respond to properties shared by many compounds. Refractive index detectors are among the best-known examples, alongside conductivity and light-scattering detectors.Both detector categories have important advantages depending on the application, sample type, and analytical goals.<h3>Common Detector Types Used in HPLC</h3>A wide variety of detectors are available for HPLC systems, ranging from highly universal devices to extremely selective and sensitive technologies.Common detector options include:<ul><li>UV/Vis detectors</li><li>Diode array detectors (DAD/PDA)</li><li>Fluorescence detectors</li><li>Refractive index detectors (RID)</li><li>Conductivity detectors</li><li>Evaporative light scattering detectors (ELSD)</li><li>Charged aerosol detectors (CAD)</li><li>Mass spectrometry detectors (MS)</li></ul>Each technology offers a different balance of sensitivity, selectivity, robustness, operational complexity, and cost.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg" alt="KNAUER: Detector overview with characteristics. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg 245w," sizes="100vw" width="1293" height="596"><h3>Selecting the Right HPLC Detector</h3>There is no universally ideal detector for all HPLC applications. The best choice depends on several factors, including analyte chemistry, matrix complexity, required detection limits, and instrument compatibility.Careful detector selection is essential for obtaining reliable and meaningful analytical results.The following considerations can help guide the selection process.<h4>1. Chemical and Physical Properties of the Analytes</h4>The chemical nature of the compounds being analyzed is one of the most important selection criteria.Questions to consider include:<ul><li>Do the analytes contain chromophores?</li><li>Are the compounds naturally fluorescent?</li><li>What is their polarity?</li><li>What is their molecular weight?</li><li>Are they volatile or non-volatile?</li></ul>Compounds containing UV-active groups are often well suited for UV/Vis detection, while fluorescent molecules can benefit from the high sensitivity of fluorescence detectors. Non-UV-active compounds may require universal detection approaches such as CAD, ELSD, or mass spectrometry.<h4>2. Required Sensitivity</h4>Detector sensitivity determines the minimum concentration that can be reliably detected and quantified.Routine quality control analyses often rely on UV/Vis detectors because they provide sufficient sensitivity for many applications while remaining relatively simple and cost-effective. However, trace-level analysis or highly complex matrices may require more advanced technologies such as:<ul><li>Fluorescence detection</li><li>Charged aerosol detection</li><li>Mass spectrometry</li></ul>These techniques offer significantly lower detection limits and greater analytical specificity.<h4>3. Detector Linearity</h4>Linearity describes how well detector response correlates with analyte concentration across a defined range.A detector with strong linearity ensures accurate quantification and simplifies calibration procedures. Poor linearity can introduce quantification errors, especially when analyzing samples across broad concentration ranges.<h4>4. Dynamic Range</h4>Dynamic range refers to the concentration interval over which the detector can reliably measure analytes.Applications involving samples with highly variable concentrations require detectors capable of maintaining accurate response over a broad range. A wide dynamic range reduces the need for repeated dilutions or multiple analytical runs.<h4>5. Selectivity</h4>Selectivity defines the detector’s ability to distinguish target analytes from other matrix components.This factor becomes particularly important for complex samples containing many interfering substances. Highly selective detectors reduce the risk of overlapping signals and false positives.Mass spectrometry is often advantageous when dealing with unknown compounds or highly complicated sample matrices because it provides structural information in addition to quantitative data.<h4>6. Budget Considerations</h4>Detector cost is another practical factor that laboratories must evaluate.UV/Vis and refractive index detectors are generally among the more economical choices, making them attractive for routine analyses and standard applications.More sophisticated technologies such as fluorescence detection, CAD, or mass spectrometry typically require:<ul><li>Higher initial investment</li><li>Additional maintenance</li><li>More complex infrastructure</li><li>Greater operational expertise</li></ul>However, the overall value of the detector should always be considered in relation to analytical performance requirements and long-term application needs.<h4>7. Instrument and Mobile Phase Compatibility</h4>The selected detector must be compatible with the operational conditions of the HPLC system.Important parameters include:<ul><li>Flow rate range</li><li>Pressure limitations</li><li>Mobile phase composition</li><li>Solvent compatibility</li></ul>Some detectors are highly sensitive to mobile phase composition or gradient changes, while others tolerate a broader range of operating conditions.Ensuring proper compatibility helps prevent baseline instability, signal interference, and reduced detector performance.<h4>8. Destructive vs. Non-Destructive Detection</h4>Another important consideration is whether the analytical workflow involves only detection or also preparative purification.Non-destructive detectors allow collected fractions to remain intact after detection, which is highly beneficial for preparative chromatography and purification workflows.Destructive detectors, by contrast, consume or alter the sample during measurement and are therefore more suitable for analytical applications where sample recovery is not required.<h4>9. Reliability and Ease of Operation</h4>Practical usability is often just as important as analytical performance.Some detectors are designed for straightforward plug-and-play operation, while others require:<ul><li>Frequent calibration</li><li>Specialized maintenance</li><li>Dedicated gas supplies</li><li>Vacuum systems</li><li>Advanced operator expertise</li></ul>Laboratories should evaluate the available technical support, user experience, and maintenance capabilities before implementing more sophisticated detection technologies.Reliable operation and manageable maintenance requirements can significantly improve laboratory productivity and reduce downtime.Choosing the correct HPLC detector starts with understanding both the sample and the analytical objective. Rather than selecting a detector simply because it is available, laboratories should ensure that the technology aligns with the chemistry of the analytes, required performance characteristics, and workflow demands.In many cases, combining multiple detectors within a single HPLC system can provide even greater analytical insight by delivering complementary information from the same chromatographic separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg" alt="KNAUER: Flow scheme for detector selection. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg 245w," sizes="100vw" width="1316" height="808">Interested in learning more? Future articles will explore individual HPLC detection techniques in greater detail, including their operating principles, application areas, and practical advantages for different analytical workflows.

A comprehensive guide for selecting the appropriate HPLC detector

News from LabRulezLCMS Library - Week 20, 2026

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Science and research

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Tosoh Bioscience

Agilent Technologies

Phenomenex

Thermo Fisher Scientific

Shimadzu Corporation

SCIEX

In this part of the course we will deal with sample preparation: why, how and which sample preparation is the most suitable for the target analyte?SPE, LLSE, or syringe filter?&nbsp;<blockquote>Presenter: Shaun Pritchard (Product Specialist Liquid Phase Separations, Agilent Technologies)Shaun Pritchard is a Product Specialist for separation products at Agilent Technologies covering the UK and Ireland. He started his career in 1989 working at a large Pharmaceutical company holding a number of roles, this is where he became passionate about HPLC finally in 1998 he took the leap to leave the lab and move to the commercial side of things and has had a number of roles within the industry until 2017 he joined Agilent.</blockquote>

Agilent Technologies: HPLC Beginner Webinar Series

Sample Preparation in HPLC

Why, how and which sample preparation is the most suitable for the target analyte?

Tailing, fronting, peak doubling are all topics that we will cover during troubleshooting.After the session, we will be able to identify causes and avoid errors.<blockquote>Presenter: Giorgio Ferlat (MSc, EMEAI IDO Product Specialist, Chemistries and Supplies, Agilent Technologies)Giorgio achieved his Master Degree in Chemistry at the University of Trieste, Italy and has over 25 years of analytical chemistry experience, both in the laboratory and in the field, with strong expertise.He has over 15 years of experience in consultative consumable sales and business development covering both national and international positions.</blockquote>

Troubleshooting and Everyday Routine (HPLC Columns)

Tailing, fronting, peak doubling are all topics that we will cover during troubleshooting. 

Overview:<ul><li>Automated clean-up using solid-phase extraction for per- and polyfluorinated alkyl substances (PFAS) (presented by Norina Aßhoff/CVUA-MEL).</li><li>PFAS method according to LOD / LOQ limits in Recommendation (EU) 2022/1431 on the monitoring of perfluoroalkyl substances in food.</li><li>In fruit, vegetables &amp; baby food: limits for PFOS: 2 ng/kg, PFOA: 1 ng/kg, PFNA: 1 ng/kg, and PFHxS: 4 ng/kg.</li><li>Validated method for animal-based foods such as eggs, milk, meat and fish.</li><li>Suitability for PFAS analysis of food contact materials made of paper.</li><li>2-SPE cartridge setup (Strata GCB 250mg + Strata-X-AW 100mg) vs. single/stacked cartridge Strata PFAS (250mg / 100mg).</li></ul>Key Learning Points:<ul><li>Automated sample preparation of PFAS in food, food contact materials and consumer goods.</li><li>Additional sample preparation and LC-MS/MS methods for water, soil, and blood/serum.</li><li>Blank value-reduced SPE materials and other specific laboratory accessories for PFAS analysis.</li><li>Optimization of LC-MS/MS analysis through modern columns and strategies for better retention of short-chain PFAS.</li></ul>Who should attend?The webinar is designed for all involved in PFAS analysis in the food, feed, environmental and clinical areas, to learn about automation and new ideas for more robust PFAS methods.<blockquote>Presenter: Norina Aßhoff, CVUA-MELPresenter: Guido Rimmel, Account Manager, PhenomenexPresenter: Georg Pfefferer, Account Manager, Phenomenex</blockquote>Please note: the webinar on PFAS will not be recorded.

Phenomenex: PFAS Analysis in Food, Vegetables and Other Matrices – Automation, Hints & Developments

PFAS Analysis in Food, Vegetables and Other Matrices – Automation, Hints & Developments

Learn about automation and new ideas for more robust PFAS methods. 

PFAS Analysis in Food, Vegetables and Other Matrices

Gain insights into how delay columns, essential to control the presence of PFAS in the laboratory, function. And understand how different delay columns impact method performance.We will host a Q&amp;A session after the presentation to address any questions or comments.<blockquote>Presenter: Landon Wiest, PhD (Product Manager - LCMS, Shimadzu Scientific Instruments)Dr. Landon Wiest is the Product Manager for LCMS at Shimadzu Scientific Instruments, leading a team of scientists who focus on applications development customer care. He has extensive experience in method and product development, research project management, and customer applications, with a strong background in liquid chromatography. At Restek Corporation, Landon led technology development teams and advanced new particle technologies, while also publishing application notes and creating educational content for webinars and short courses.He earned a Ph.D. in Analytical Chemistry from Brigham Young University, where he developed patented diamond/carbon-based chromatographic particles. Recognized for his contributions to chromatography, Landon was nominated for the Csaba Horváth Award. Fluent in Danish and knowledgeable in German, Landon excels at communicating complex scientific concepts and is dedicated to advancing scientific knowledge. Presenter: Ruth Marfil-Vega, PhD (Sr Market Manager – Environmental, Shimadzu Scientific Instruments)Dr. Ruth Marfil-Vega has 20 years of expertise in environmental chemistry and engineering in the US and Europe. She has worked with the EPA and the utilities industry to design and execute comprehensive solutions for addressing new environmental and water quality issues, including PFAS, regulated and unregulated DBPs, and other emerging contaminants. Since Ruth joined Shimadzu five years ago, she has continued to work with environmental stakeholders to ensure that analytical instrumentation and workflows support their success. She earned a BSc in Chemistry from the University of Valladolid (Spain) and a PhD in Environmental Science and Engineering from the University of Cincinnati.</blockquote><h3>PFAS in Focus</h3>Welcome to PFAS in Focus, a comprehensive and exclusive webinar series sponsored by Shimadzu, covering the basics and beyond about the analysis of Per- and Polyfluoroalkyl Substances (PFAS) in the environment.Join our experts, live or on-demand after the original air date, to demystify the challenges of PFAS analysis and gain the insights you need to:<ul><li>Manage PFAS contamination in your laboratory.</li><li>Master standardized methods for PFAS testing.</li><li>Discover new analytical workflows to maximize the performance of your LC-MS/MS for PFAS analysis.</li></ul>Who should attend:<ul><li>Environmental industry professionals and academics currently working on PFAS analysis or looking to establish the application in their labs.</li><li>Representatives from drinking and wastewater utilities.</li><li>LC–MS users from any field interested in expanding their applications to include PFAS testing.</li></ul>

Mastering PFAS Control: Transform Your Laboratory Practices

Gain insights into how delay columns, essential to control the presence of PFAS in the laboratory, function.

Innovating for the future with cutting-edge solutionsGiven the speed and scale of progress with Artificial Intelligence (AI) in today’s world, researchers and professionals across various scientific fields from the Danaher group of companies have stepped up in the game of AI and advanced technology to seek breakthrough solutions to age-old problems.<h3>Agenda</h3><h5>Introduction to Danaher</h5><h5>3D biology and the paradigm shift in next generation drug discovery</h5><ul><li>Presented by Molecular Device</li></ul><h5>AIVIA - introduction to AI based image analysis</h5><ul><li>Presented by Leica Microsystems</li></ul><h5>Analytical advances in the characterization of mRNA</h5><ul><li>Presented by Phenomenex</li></ul><h5>Unlocking the power of data with IDBS</h5><ul><li>Presented by IDBS</li></ul><h5>The power of AI: from planning and the lab to success in life sciences</h5><ul><li>Presented by Abcam</li></ul><h5>Practical approaches to EV characterization and purification: leveraging nano Flow Cytometry and ultracentrifugation for clinical and commercial success</h5><ul><li>Presented by Beckman Coulter Life Sciences</li></ul><h5>AI-MS/MS-AI mass spectrometry application with AI</h5><ul><li>Presented by SCIEX</li></ul><h5>Work smarter, not harder: emerging lessons on data in biopharma R&amp;D</h5><ul><li>Presented by Genedata</li></ul><h5>In silico process development with mechanistic chromatography modelling</h5><ul><li>Presented by Cytiva</li></ul><h5>CRISPR meets NGS: the powerful duo for drug discovery</h5><ul><li>Presented by IDT</li></ul><h3>Presenter:</h3><ul><li>Gokhale, Aarti Arun, Ph.D (Sr Manager, Gene Reading, APAC, IDT)</li><li>Teo Chong Kok Ph.D (Scientific Account Manager, Genedata)</li><li>Edward Lim (General Manager, SEA, Beckman Coulter Life Sciences)</li><li>Taryn Guinan, Ph.D (Sr Applications Specialist, ANZ, Leica Microsystem)</li><li>Edward Wong, Ph.D (Sr Manager, Gene Writing &amp; Editing, APAC, IDT)</li><li>Stephan Waldert, Ph.D (Director, Data Science &amp; AI, Abcam)</li><li>Manish Manakchand (Commercial Director, Asia, IDBS)</li><li>Namrata Saxena (Technical Manager, Phenomenex)</li><li>Hans Lin (Field Application &amp; Market Development Specialist, SCIEX)</li><li>Mok Wei Chuan (Go Silico Sales Specialist, APAC, Cytiva)</li><li>Minho Tak, Ph.D (Sr Application Scientist, JAPAC, Molecular Device)</li></ul>

SCIEX: AI and technology advancements from Danaher

AI and technology advancements from Danaher

In this live webinar, let’s explore how Danaher group of companies leverage AI and technologies in providing advanced solutions.

In this educational back-to-school webinar, James Grinias (University of Rowan) and Frank Steiner (Thermo Scientific) discuss strategic approaches for development, optimization and transfer of legacy and modern HPLC methods, utilizing gradient elution.Discussion topics and data on how to more easily move to core-shell column technology, how to modify gradient methods effectively, and how to work within the framework of evolving rules for method transfer such as USP 621.We also discuss using the right tool to reduce the impact of method transfer across HPLC platforms. &nbsp;Key learning objectives:<ul><li>&nbsp;Understanding gradient-based LC separations</li><li>Scalable parameters in pharmacopeia method modernization</li><li>&nbsp;Tricks and tips on method development and transfer</li></ul>If you are unable to attend, please register to ensure you will receive the link to the recording.<blockquote>Presenter: James Grinias (Associate Professor, Department of Chemistry &amp; Biochemistry, Rowan University)James P. Grinias, Associate Professor, Rowan University, teaches analytical and bioanalytical chemistry. His research focuses on UHPLC columns and instrument miniaturization. His work has been recognized with recognition from the American Chemical Society Subdivision on Chromatography and Separations Chemistry, LCGC magazine, and The Analytical Scientist magazine. Presenter: Frank Steiner (Sr. Manager of Product Applications and Scientific Advisor, Chromatography and Mass Spectrometry, Analytical Instrument, Thermo Fisher Scientific)Dr. Frank Steiner is Sr. Manager of Product Applications and Scientific Advisor in the HPLC organization of Thermo Fisher Scientific and coordinates scientific collaborations with external partners to advance UHPLC technologies and applications.</blockquote>

Thermo Fisher Scientific: The real impact of HPLC and column parameters

The real impact of HPLC and column parameters

We will discuss strategic approaches for development, optimization and transfer of legacy and modern HPLC methods, utilizing gradient elution.

Choosing the appropriate column for your polymer analysis can be a challenge. Gel Permeation Chromatography (GPC) and Size Exclusion Chromatography (SEC) are separation techniques often used for polymers to determine the molecular weight and molecular weight distribution. &nbsp;Whether working with organic or aqueous mobile phase conditions, there are numerous factors and criteria to consider beyond the MW range when selecting the ideal GPC or SEC column.These include:<ul><li>individual pore, MIXED, and multiporous packing features and benefits</li><li>particle and pore size considerations&nbsp;</li><li>column chemistries and polymer sample types</li></ul> Additionally, we will cover best practices for selecting solvents, choosing calibration standards, and for using columns together in series. Application examples will be given. With a diverse range of column options available, we will demonstrate how to maximize performance through your GPC/SEC column selection<blockquote>Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.</blockquote>

Agilent Technologies: Becoming a Better Chromatographer - Educational Webinar Series

Optimize Your Polymer Analysis by Selecting the Ideal GPC/SEC Column

GPC & SEC are separation techniques often used for polymers to determine the molecular weight and molecular weight distribution.

Polymer Analysis by Selecting the Ideal GPC/SEC Column

While connecting capillaries are not among the primary liquid chromatography modules, they can still play an important role in the LC process and its results.&nbsp;Something as simple as a bad connection can potentially cause chromatography problems, such as peak shape issues, that may take you a long time to troubleshoot. Careful attention to making a good connection can reduce frustration and save time troubleshooting.&nbsp;In this webinar, we will discuss various types of capillaries and fittings and provide helpful information on selection criteria. &nbsp;We will also provide guidelines for troubleshooting issues related to capillaries and fittings.<blockquote>Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.</blockquote>

LC Capillaries and Fittings

We will discuss various types of capillaries and fittings and provide helpful information on selection criteria.

Protein-based therapeutics such as monoclonal antibodies are widely developed and used by the biopharmaceutical industry; in 2023, mAb-based therapeutics represented over half of the Top Ten best-selling drugs of the year, globally, and are one of the fastest growing modalities. However, these large molecules pose unique challenges in relation to sample preparation and analytical characterisation, making robust and fit-for-purpose methods essential to achieve high quality results.This December, we invite you to connect with fellow scientists to learn more about the latest innovations in Protein analysis at our ProteinConnect Virtual Forum. Through a selection of complementary presentations and resources, our specialists will share insights into the present and future of protein characterisation, including:<ul><li>How workflow solutions can address mAb CQA analysis</li><li>Hints and tips for optimising protein bioseparations</li><li>The latest tools and technologies to enhance your protein analysis</li><li>Novel approaches to support online analysis and production of mAbs</li><li>A look to the future – emerging trends in Biopharma analysis</li><li>And much more! agilent ms</li></ul><h5>Agenda 10 December 2024</h5>10:30 a.m. Approaching The Analysis Of mAbsAnd Protein-based Therapeutics: An Introduction11:15 a.m. Optimizing Protein Therapeutic Analysis with Agilent Columns: Innovations And Applications12:15 p.m. Online LC: A Powerful Tool For Process Monitoring During The Production Of mAbs13:00 p.m. ProteoAnalyzer: Analysis of NIST mAbReference Material By Parallel SDS-Capillary Electrophoresis

Agilent Technologies: ProteinConnect: Linking Science, Innovation and Therapeutics

ProteinConnect: Linking Science, Innovation and Therapeutics (Day 1)

We invite you to connect with fellow scientists to learn more about the latest innovations in Protein analysis at our ProteinConnect Virtual Forum.

ProteinConnect: Linking Science, Innovation and Therapeutics

Christian Rohrer, Director of Sales &amp; Support – EMEA at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.On December 10, Sebastian Thuermann, Product Manager MCC, and Carsten-Rene Arlt, Application Scientist, Process Instruments at Tosoh Bioscience, will demonstrate that multi-column chromatography (MCC), including continuous capture and simulated moving bed (SMB) chromatography, offer cost-effective alternatives with higher productivity and lower resource consumption for pharmaceutical manufacturing.After the webinar, ask your questions during the live Q&amp;A session.<blockquote>Presenter: Sebastian ThuermannPresenter: Carsten-René ArltPresenter:&nbsp;Christian Rohrer</blockquote>

Tosoh Bioscience: Tosoh Tuesday - Continuous Chromatographic mAb Polishing via Step Gradient-SMB

Tosoh Tuesday - Continuous Chromatographic mAb Polishing via Step Gradient-SMB

MCC and SMB chromatography, offer cost-effective alternatives with higher productivity and lower resource consumption for pharmaceutical manufacturing

LCMS webinars focusing on Consumables - page 9