Understanding the fundamentals of the on-off retention mechanism of oligonucleotides and their application to high throughput analysis

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 13th January 2025? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications by <strong>Thermo Fisher Scientific,</strong> <strong>Shimadzu</strong>,<strong> </strong>a presentation by <strong>Agilent Technologies</strong>, a manual by <strong>Metrohm,</strong> and a poster from <strong>ASMS </strong>by <strong>Waters Corporation</strong>!</p><h4><strong>1. Thermo Fisher Scientific: High-resolution DIA proteomics workflow for single-cell samples on the Orbitrap Astral mass spectrometer</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/28638"><strong>Full PDF for download</strong></a></li></ul><p>Assess proteome coverage and sample throughput performance for single-cell samples using a library-free and library-based data-independent acquisition (DIA) method on the Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer.</p><h5><strong>Introduction</strong></h5><p>In recent years, there has been a shift to analyzing smaller sample quantities, driven by the interest in profiling the proteome of individual cells. This increased focus on LC-MS analysis of limited sample amounts, e.g., single cells, requires the highest possible sensitivity. At the same time, throughput and chromatographic performance must be preserved to generate sufficiently high-quality data from large data sets to draw meaningful conclusions. This trend toward analyzing smaller sample quantities reflects the need to understand the heterogeneous nature of biology through the dissection of complex systems into their individual parts or, specifically, individual cells. Traditional proteomics approaches read out the average of a sample while single-cell proteomics provides insights into the exact nature of each of the components in such samples.</p><p>The Orbitrap Astral mass spectrometer delivers a new standard for single-cell samples with deeper proteome coverage and faster throughput. It enables these capabilities through the synchronized acquisition of high-resolution and high-dynamic range full scan spectra with the Thermo Scientific™ Orbitrap™ mass analyzer and high speed and sensitivity MS/MS spectra with the Thermo Scientific™ Astral™ mass analyzer. The Thermo Scientific™ Vanquish™ Neo UHPLC system delivers maximum performance in terms of precise, low flow rates and gradient formation, as well as low injection volumes—an important benefit for single-cell proteomics and limited sample amounts.</p><p>The Thermo Scientific™ μPAC™ Neo HPLC columns with novel micro-pillar array deliver highly reproducible chromatographic separation and increased sensitivity with maximum resolution.<br>The Thermo Scientific™ FAIMS Pro Duo interface enhances sensitivity by removing background signals.</p><p>In this technical note, we present a single-cell proteomics workflow (Figure 1). To first evaluate sensitivity and quantitative precision and accuracy and linearity of the workflow, a dilution series from 5 ng to 50 pg of the Thermo Scientific™ Pierce™ HeLa protein digest standard was analyzed.</p><h4><strong>2. Shimadzu: Automatic Optimization of Gradient Conditions by AI Algorithm - Consecutive Optimization at Different Column Oven Temperatures</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/28642"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>The AI algorithm of LabSolutions MD can automatically optimize gradient conditions to greatly reduce labor of LC method development.</li><li>Gradient conditions are automatically optimized at different column oven temperatures consecutively.</li><li>Automatic optimization of gradient conditions can be applied not only to new method development, but also to existing method to efficiently improve resolution.</li></ul><h5><strong>Introduction</strong></h5><p>In the typical LC method development, the process begins with “preparation” which includes mobile phase preparation, column installation, and creation of analysis schedules, then the analysis is started. After that, the acquired data is analyzed and “preparation” for the subsequent analysis is carried out, followed by starting the next analysis again. The method development progresses by repeating these processes, but in addition to the significant time required to repeatedly create analysis schedules, expertise in chromatography is necessary to explore optimal conditions based on data analysis. In other words, typical method development requires “human intervention”. Therefore, eliminating human involvement and automating such method development processes would be desirable to improve labor efficiency. This article introduces an approach for automatically optimizing gradient conditions at different column oven temperatures consecutively, identifying the combination of temperature and gradient conditions that meet the resolution criteria.</p><h5><strong>Conclusion</strong></h5><p>Automatic optimization of gradient conditions using AI algorithm of LabSolutions MD was applied to a model sample (mixture of seven compounds of small molecule) at different column oven temperatures. As a result, the temperature and gradient conditions that met the resolution criteria were successfully explored. In method development, human intervention, such as analysis batch creation and data analysis, is required to optimize gradient conditions. LabSolutions MD can provide significant labor savings in this area. For more information on LabSolutions MD, please refer to the Technical Report <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/c190_e284_1ca86b48ea/c190-e284.pdf">“Efficient Method Development Based on Analytical Quality by Design with LabSolutions MD Software (C190-E284)”</a>.</p><h4><strong>3. Agilent Technologies: Optimize Your Polymer Analysis by Selecting the Ideal GPC/SEC Column</strong></h4><ul><li>Presentation</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/28578"><strong>Full PDF for download</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4481"><strong>Recorded webinar</strong></a></li></ul><p>&nbsp;</p><ul><li><strong>GPC/SEC Separation Mechanism</strong></li><li><strong>Conventional GPC/SEC Workflow</strong></li><li><strong>Considerations for GPC/SEC Column Selection (Key questions to ask)</strong><ul><li>What polymer are you analysing?</li><li>Which solvent (or solvents) is your polymer soluble in?</li><li>What is the expected molecular weight range of your polymer?</li><li>What is the requirement for your analysis or what would you like to improve about your existing GPC/SEC separation?<ul><li>Resolution is important</li><li>Reproducibility of sample chromatography and results</li><li>Speed of analysis or sample throughput is something to improve on</li></ul></li></ul></li><li><strong>Further considerations</strong><ul><li>Know the properties of the sample</li><li>Be familiar with the properties of the columns being considered</li><li>It is important to balance polarities for the sample, solvent, and column packing</li></ul></li><li><strong>Common Column Chemistries for GPC/SEC</strong></li><li><strong>Agilent Magic Triangles</strong></li><li><strong>Column Selection: Porosity</strong></li><li><strong>Individual Pore Versus MultiPorous Particle</strong></li><li><strong>Column Selection: Particle size</strong></li><li><strong>Columns for Low Polarity Organic Eluents</strong></li><li><strong>Columns for Medium Polarity Organic Eluents</strong></li><li><strong>Columns for High Temperature GPC/SEC</strong></li><li><strong>Columns for Fluorinated Organic Eluents</strong></li><li><strong>Columns for Aqueous Eluents: Neutral or weak Anions</strong></li><li><strong>Columns for Aqueous Eluents: Polycations and Strong Polyanions</strong></li><li><strong>Column Selection: Additional Considerations</strong></li><li><strong>Columns in Series</strong></li><li><strong>Wrongly Coupled Columns</strong></li><li><strong>Combination of Individual Pore Size Columns</strong></li><li><strong>Column Selection and Importance of Solvent Choice</strong></li><li><strong>Solvent Selection</strong></li><li><strong>Successful Solvent Choice</strong></li><li><strong>Eluent Modification in Organic GPC</strong></li><li><strong>Common Questions Around GPC/SEC Standards</strong></li><li><strong>Agilent Polymer Calibration Standard Offerings</strong></li><li><strong>Well Characterized Polymer Standards</strong></li><li><strong>Easivial Certificate of Analysis</strong></li><li><strong>Common Questions Around SEC Standards</strong></li><li><strong>Installation and Care of Your Column</strong></li><li><strong>Before Your Analysis</strong></li><li><strong>Column Selection – Additional considerations</strong></li><li><strong>System Tubing</strong></li><li><strong>Your GPC/SEC Toolbox</strong></li></ul><h4><strong>4. Metrohm: Installation Instruction for ProfIC Vario 7 Cation</strong></h4><ul><li>Manual</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/28584"><strong>Full PDF for download</strong></a></li></ul><p>The ProfIC Vario 7 Cation is a Professional IC Vario system with Inline Dilution and Inline Dialysis for the fully automatic determination of cations in high concentrations in matrices containing emulsions. This system enables users to dilute the sample and eliminate droplets of fat or organic solvents or particles prior to the analysis.</p><h4><strong>5. Waters Corporation: IMPROVED TISSUE HOMOGENIZATION AND SPE-BASED SAMPLE PREPARATIONS FOR THE QUANTITATIVE LC-MS ANALYSIS OF OLIGONUCLEOTIDE THERAPEUTICS</strong></h4><ul><li>Poster/ASMS</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/27580"><strong>Full PDF for download</strong></a></li></ul><p>Efficient extraction of drug analytes is a critical aspect of drug metabolism and pharmacokinetics (DMPK) studies. This has long applied to small molecules and still applies to the bioanalysis of oligonucleotides. Oligonucleotide drugs and their metabolites must be quantified within both biofluids and tissue samples. The newest of oligonucleotide drugs are both extensively modified and conjugated. These modified residues and conjugate moieties can complicate extraction recovery and reproducibility. In this work, we report several key insights on how to achieve improved extractions. Protocols for solvent-assisted proteinase K sample pre-treatments are investigated using a weak anion exchange (WAX) microplate-based solid phase extraction (SPE) device. Direct injection LC-MS quantitation is demonstrated for all three antisense oligonucleotides (ASOs).</p><h5><strong>CONCLUSIONS</strong></h5><ul><li>Selective extraction and elution of ASO oligonucleotides is feasible from multiple tissue types.</li><li>Extracted ASOs are amenable for either full scan mode analysis on benchtop small footprint TOF MS or MRM analysis by Triple Quad MS</li><li>Reproducible and repeatable data were collected even without the application of an internal standard</li><li>One novel aspect of the SPE eluate is that no further sample preparation is required for subsequent LC-MS analysis saving time and avoiding sample losses during drying and reconstitution</li><li>Eluted nucleic acid components can be readily analyzed by regular ion-pairing reversed phase chromatography and UV/MS-based detection and quantitation</li><li>This robust tissue extraction protocols for improved LC-MS based bioanalysis of ASO drugs was repeatable and highly reproducible across multiple batches of SPE sorbent, providing confidence in the suitability of the optimized protocols</li></ul>

News from LabRulezLCMS Library - Week 3, 2025

<p><strong>In the week of January 20, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over&nbsp;</p><p><strong>4 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Characterization and sequence mapping of large RNA and mRNA therapeutics using HRAM</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4580">Registration</a></div></li></ul></div><p>The presentation showcases the innovative partial T1 digest strategy developed at the University of Sheffield for sequencing mRNA therapeutics.</p></div><h5><strong>2.&nbsp;SelectScience/Agilent Technologies: From Start to Finish: How Agilent Can Support Better Decisions in IVT mRNA-Based Biopharmaceutical Analysis</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4674">Registration</a></div></li></ul></div><p>We will discuss how automated electrophoresis and LC/MS systems solutions from Agilent provide reliable insights for IVT mRNA workflows at many steps.</p></div><h5><strong>3.&nbsp;Phenomenex: LC Troubleshooting Quick Guide: Spot Problems Fast &amp; Fix Them Like a Pro!</strong></h5><div><div><ul><li><div>Th, 23.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4676">Registration</a></div></li></ul></div><p>In this webinar - General troubleshooting approach, HPLC troubleshooting steps and diagnosing tips and preventive maintenance and column care.</p></div><h5><strong>4.&nbsp;Thermo Fisher Scientific: State-of-the-art E&amp;L is not rocket science</strong></h5><div><div><ul><li><div>Th, 23.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4581">Registration</a></div></li></ul></div><p>Ulli Honester, a team leader from Solvias, presented on advancements in mass spectrometry for identifying extractables and leachables (E&amp;L).</p></div><h5><strong>5.&nbsp;Waters Corporation: Empower Tips: Ask Me Anything About Navigating Data Governance - Risk and Vigilant Monitoring</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4550">Registration</a></div></li></ul></div><p>We will explore critical aspects of data governance and critical thinking, insights and strategies to help navigate the current environment, evolving your approach and modernizing your way of working.</p></div><h5><strong>6.&nbsp;Evosep Biosystems: A beginner’s guide to Evosep 2025</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4548">Registration</a></div></li></ul></div><p>This webinar is your opportunity to gain a comprehensive understanding of the Evosep One’s key features and how it can seamlessly integrate into your current processes.</p></div><h5><strong>7.&nbsp;Shimadzu Corporation: ASTM D8421 and D8535: Cost Effective Methods for Rapid PFAS Testing in Wastewater and Soil</strong></h5><div><div><ul><li><div>Th, 23.1.2025 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4465">Registration</a></div></li></ul></div><p>Discover simplified standardized methods for PFAS analysis based on co-solvation as sample preparation.</p></div>

Webinars LabRulezLCMS Week 4/2025

Article

Webinars

Application

Scientific article

Various

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Metrohm

<p><strong>From applied research to corrosion monitoring to quality control of boiler feed water in the power industry – voltammetry is widely used for highly selective and sensitive analysis of heavy metals. Now, a new generation of mercury-free sensors developed by Metrohm makes this method «green» and hence compliant with increasingly tougher environmental regulations in many countries.</strong></p>
<p>Join Jakub Tymoczko, Application Specialist at Metrohm International Headquarters, in his webinar on December 13, as he explains how to perform «green voltammetry» in the laboratory as well as in the field for fast determination and speciation of heavy metals ranging from arsenic to zinc.</p>
<p>Key learnings of this webinar:</p>
<ul>
<li>The principle behind the new generation of Metrohm&#39;s mercury-free sensors</li>
<li>The inherent benefits of voltammetry for heavy metal analysis (lower costs, easier to use, faster results than by competing methods, e.g., ICP-MS)</li>
<li>Typical application examples from different fields (research, process control, monitoring of drinking water)</li>
<li>Automation possibilities for high throughput analysis of large sample series</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Jakub Tymoczko, PhD (Application Specialist Voltammetry, Metrohm International Headquarters)</p>
</blockquote>
<blockquote>
<p>Dr. Jakub Tymoczko has worked for the Competence Center Voltammetry at Metrohm International Headquarters in Switzerland since 2015. In this time, he has been in charge of developing Metrohm&#39;s new generation of mercury-free sensors and methods for heavy metal detection. Jakub has a Master in biochemistry and received his PhD in electrochemistry on the engineering of model electrocatalysts for energy conversion and storage from the Ruhr-University in Bochum.</p>
</blockquote>


Metrohm: Voltammetry goes green – mercury-free analysis of heavy metals

Voltammetry goes green – mercury-free analysis of heavy metals

How to perform «green voltammetry» in the laboratory as well as in the field for fast determination and speciation of heavy metals ranging from arsenic to zinc.

Green voltammetry – mercury-free analysis of heavy metals

<p><strong>From applied research to corrosion monitoring to quality control of boiler feed water in the power industry – voltammetry is an established method for highly selective and sensitive analysis of heavy metals. Now, a new generation of mercury-free sensors developed by Metrohm makes this method «green» and hence compatible with increasingly tougher environmental regulations in many countries.</strong></p>
<p>Join Jakub Tymoczko, Application Specialist at Metrohm International Headquarters, in his webinar on November 2 and learn how how you can perform «green voltammetry» in the laboratory as well as in the field for fast determination and speciation of heavy metals ranging from arsenic to zinc.</p>
<p>Key learnings of this webinar:</p>
<ul>
<li><strong>The principle behind the new generation of mercury-free sensors from Metrohm</strong></li>
<li><strong>The inherent benefits of voltammetry for heavy metal analysis such as lower costs, easier to use, faster results than by competing methods, e.g., ICP-MS</strong></li>
<li><strong>Typical application examples from different fields (research, process control, monitoring of drinking water)</strong></li>
<li><strong>Automation possibilities for high throughput analysis of large sample series</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Jakub Tymoczko (Application Specialist Voltammetry, Metrohm International Headquarters)</p>
</blockquote>
<blockquote>
<p>Dr. Jakub Tymoczko has worked for the Competence Center Voltammetry at Metrohm International Headquarters in Switzerland since 2015. In this time, he has been in charge of developing Metrohm&#39;s new generation of mercury-free sensors and methods for heavy metal detection. Jakub has a Master in biochemistry and received his PhD in electrochemistry on the engineering of model electrocatalysts for energy conversion and storage from the Ruhr-University in Bochum.</p>
</blockquote>


Metrohm: Green voltammetry – a new generation of sensors for mercury-free analysis of heavy metals

Green voltammetry – a new generation of sensors for mercury-free analysis of heavy metals

How you can perform «green voltammetry» in the laboratory as well as in the field for fast determination and speciation of heavy metals.

<p>This webinar is offered several times. Select the date and time that works best for you.</p>
<ul>
<li><strong>Tue, Mar 19, 2024, 10:00 AM - 11:00 AM CET</strong></li>
<li><strong>Tue, Mar 19, 2024, 3:30 PM - 4:30 PM CET</strong></li>
</ul>
<p><strong>Ever wondered about what&#39;s inside your potentiostat and whether it really matters? In this webinar we&#39;ll answer questions you&#39;ve always wanted to ask, explain how a potential is applied or a current measured in a conventional three-electrode setup. Do you know what the compliance voltage or the bandwidth of the instrument is, and why one should care?</strong></p>
<p>With a good understanding of how the instrument is made and operates you’ll be able to make sense of the data it provides, troubleshoot common electrochemical experiments, and boost your confidence in the lab. </p>
<p>This webinar is accessible to anyone: no background in electronics is required. </p>
<blockquote>
<p><strong>Presenter:</strong> Thomas Touzalin, PhD. (Product Manager at Metrohm Autolab)</p>
</blockquote>
<blockquote>
<p>Helping the electrochemistry researchers achieve their goals by providing the right solution for any research problems as well as offering technical support for the Autolab product range.</p>
</blockquote>
<p><strong>Surprise:</strong> We will be giving away the <strong>&quot;A guide to flawless electrochemical measurements&quot;</strong> lab poster, a step-by-step guide to <strong>address common issues you may encounter when using a potentiostat</strong>. You can hang it in your lab to ensure the success of every experiment. If you would like to stand a chance to win one through a lucky draw, please indicate below.</p>


Metrolab: Beyond the black box: how a potentiostat works

Beyond the black box: how a potentiostat works

In this webinar we'll explain how a potential is applied or a current measured in a conventional three-electrode setup.

<p><strong>CVS (Cyclic Voltammetric Stripping) is the preferred method for monitoring the concentration of suppressors, brighteners, and levelers used in the production of high-quality printed circuit boards (PCBs) and in advanced packaging of semiconductors. Automating this monitoring can help manufacturers to significantly save time and effort while helping them to keep pace with increasing numbers of samples.</strong></p>
<p>In her webinar, Barbara Zumbrägel, Product Manager Voltammetry at Metrohm International Headquarters, will present the latest technology to take fully automated quality control in PCB manufacturing with CVS to the next level of performance, efficiency, and convenience. Following the webinar, Barbara will be available for a Q&amp;A Session to answer any questions you may have.</p>
<p><strong>Key learnings of this webinar:</strong></p>
<ul>
<li>How automated sample transfer and dosing can make your determination of suppressors, brighteners, and levelers more efficient</li>
<li>How to handle the increasing number of auxiliary solutions with an automated pipetting system</li>
<li>How to optimize your workflow by customizing methods in the viva software to meet the specific requirements of your analysis protocol</li>
</ul>
<p><strong>Who should attend?</strong></p>
<ul>
<li>Lab technicians and lab managers from the PCB, PWB, and semiconductor manufacturing industries</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Barbara Zumbrägel (Product Manager Voltammetry, Metrohm International Headquarters)</p>
</blockquote>
<blockquote>
<p>Barbara Zumbrägel earned her diploma in chemistry from Technische Universität Clausthal, Germany, and joined Metrohm International Headquarters in 1999 as a product and application specialist for voltammetry. Since 2003, she has also played a significant role in the ongoing development of the Metrohm portfolio of CVS products and applications. In 2006, Barbara was promoted to Product Manager Voltammetry.</p>
</blockquote>


Metrohm: Next level of CVS automation - how to increase the efficiency of your routine analysis

Next level of CVS automation - how to increase the efficiency of your routine analysis

Fully automated quality control in PCB manufacturing with CVS for the next level performance, efficiency, and convenience.

LCMS webinars focusing on Voltammetry | LabRulez LCMS