MULTI-2D LC×LC-ESI/TPI-DUAL SOURCE-QTOF-MS for complex samples analysis (Oliver Schmitz, MDCW 2025)

🎤 Presenter: Oliver Schmitz (University of Duisburg-Essen, Essen, Germany)

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💡 Book in your calendar: 17th Multidimensional Chromatography Workshop (MDCW) 13 - 15. January 2026

Abstract

Even though mass spectrometers, especially in combination with ion mobility spectroscopy, are becoming increasingly powerful and are characterized by an impressive separation performance in a very short analysis time, ion suppression poses a serious problem with these analysis systems.

Here we show the influence of ion suppression using 1D and 2D LC methods and present an LC×LC method with two stationary phases in the 2nd dimension that can be freely selected during the analysis to increase the chromatographic separation power and reduce the ion suppression. For the subsequent mass spectrometric detection, it is also possible to switch between two different ion sources during the measurement in order to always be able to use the optimum ionization method. The construction and optimization of this dual ion source, consisting of electrospray ion source (ESI) and tube plasma temperature ion source (TPI) is also discussed.

Finally, first results of the coupling of 2D-LC and GC methods with ion mobility mass spectrometry are presented.