Why avoid C18 for polar analytes? Overview of HILIC principles, mobile phase composition, gradient strategy, pH testing, and practical tips.

Proper drying of the SPE sorbent is a critical step to achieve high sensitivity, good reproducibility, and maximum analyte recovery, especially for complex sample matrices.

An SPE technical tip focused on identifying the sources of interferences and extra peaks observed after sample extraction.

Although 100% recovery is expected in SPE, values above 100% are sometimes observed. This article explains the most common causes and provides practical guidance on how to identify and resolve them.

A technical guide to troubleshooting low analyte recovery in SPE. Explains relative vs. absolute recovery and provides practical solutions for conditioning, loading, and sorbent selection.

Phospholipids in serum and plasma can cause ion suppression, reduced MS sensitivity, and shorter column lifetime. Learn why phospholipid removal is critical for reliable LC-MS analysis.

How can neutral analytes be efficiently concentrated in the presence of ionized acidic or basic contaminants? This SPE tip explains how mixed-mode cartridges improve selectivity and cleanup.

Practical guide to oligonucleotide sample preparation using SPE. Learn how to optimize wash and elution steps for clean extracts and reliable LC-MS bioanalysis.

A streamlined workflow for N-linked glycan sample preparation, combining PNGase F digestion, fluorescent labeling, and HILIC SPE clean-up for reliable HPLC and LC-MS analysis.

Matrix effects can suppress or enhance analyte signals and compromise accuracy. Learn how sample prep techniques—especially SPE with Strata-X PRO—reduce phospholipid interference and improve LC-MS results.