Pesticides are chemicals that are very closely monitored and may not always be easy for an analytical chemist to monitor and detect.
Thanks to their use in agriculture and subsequently also their cycle in environment we can find them almost everywhere. The variety of analyzed matrices is so wide, detection limits are pushing down and the field of analytical chemistry is also under pressure on sample throughput, and thus also the efficiency and economy of methods.
Instrumental techniques are also being developed hand in hand with these requirements, and we will look at the latest possibilities of liquid chromatographs (LC) in connection with triple quadrupole mass spectrometers (LC/QQQ) in this overview.
We will focus on 2 main areas of these analyzes, namely food and the environment, in which you can then filter in more detail in the library, eg by combination with various separation techniques such as Supercritical Fluid Chromatography (<a href="https://lcms.labrulez.com/library?search=pesticides&amp;instrumentationItems=35-32-31-38&amp;date=2020-2021&amp;publicationTypeItems=1-2-7-5-4">SFC</a>) or take a closer look at <a href="https://lcms.labrulez.com/library?search=pesticides&amp;instrumentationItems=35-32-31-10&amp;date=2020-2021&amp;publicationTypeItems=1-2-7-5-4">sample preparation</a> and suitable <a href="https://lcms.labrulez.com/library?search=pesticides&amp;instrumentationItems=35-32-31-20-34&amp;date=2020-2021&amp;publicationTypeItems=1-2-7-5-4">HPLC columns</a>.
All documents can be found <a href="https://lcms.labrulez.com/library?search=pesticides&amp;instrumentationItems=35-32-31&amp;date=2020-2021&amp;publicationTypeItems=1-2-7-5-4">directly for download in our LabRulezLCMS library</a> and to complement possible analytical techniques you can also explore similar applications using gas chromatographs (GC) in conjunction with triple quadrupole mass spectrometers (GC/QQQ) in an article on the <a href="https://gcms.labrulez.com/article/1275">LabRulezGCMS portal </a>. 
Today, you will find almost 13,000 documents in the field of analytical chemistry and separation techniques in libraries, and their number is growing every day.
<h3>Food &amp; Agriculture LC/QQQ</h3>
<h4>Applications (27)</h4>
<ul>
<li>Determination of Anionic Polar Pesticides in Grapes using UPLC-MS with Anionic Polar Pesticide Column | 2020 | Waters
</li>
<li>Multiresidue Pesticide Analysis in Black Tea and Orange Matrix Using the Agilent 6470B Triple Quadrupole LC/MS Systém | 2020 | Agilent Technologies
</li>
<li>Evaluation of the Performance of an LC-MS/MS Method for the Determination of Anionic Polar Pesticide Residues in Crops and Foodstuffs Using an Interlaboratory Study | 2021 | Waters
</li>
<li>Multimethod Pesticide Analysis with the Agilent 1260 Infinity II Prime LC System and Agilent Ultivo Triple Quadrupole LC/MS | 2020 | Agilent Technologies
</li>
<li>Taking Advantage of the Orthogonal Selectivities of Agilent InfinityLab Poroshell 120 C18 Columns for Method Development at High pH | 2020 | Agilent Technologies
</li>
<li>Determination of Cationic Polar Pesticides and Plant Growth Regulators Using UPLC-MS/MS with the ACQUITY UPLC BEH Amide Column | 2021 | Waters
</li>
<li>Quantitation of Pesticide Residues in Milk Using the Agilent 6470 Triple Quadrupole LC/MS | 2021 | Agilent Technologies
</li>
<li>Comprehensive LC/MS/MS Workflow of Pesticide Residues in Food Using the Agilent 6470 Triple Quadrupole LC/MS System | 2020 | Agilent Technologies
</li>
<li>Analysis of Multiclass Multiresidue Pesticides in Milk Using Agilent Captiva EMR—Lipid with LC/MS/MS and GC/MS/MS | 2020 | Agilent Technologies
</li>
<li>Quantification of acetamiprid and prochloraz in black pepper using the SCIEX Triple Quad™ 3500 LC-MS/MS Systém | 2020 | SCIEX
</li>
<li>Multiresidue Method for the Quantification of Pesticides in Fruits, Vegetables, Cereals and Black Tea using UPLC-MS/MS | 2020 | Waters
</li>
<li>Analysis of Residual Pesticides (No. 1: in Soybeans) Using Triple Quadrupole LC/MS/MS <LCMSTM-8060> | 2020 | Shimadzu
</li>
<li>Automating the Preparation of Matrix Matched Calibration Standards for the Analysis of Food Contaminants by LC/MS/MS | 2020 | GERSTEL
</li>
<li>Determination of Chlorate and Perchlorate in Infant Milk Using Waters Anionic Polar Pesticide Column and UPLC-MS/MS | 2020 | Waters
</li>
<li>Trace-Level Analysis of Chlorates in Milk and Infant Formula | 2020 | Agilent Technologies
</li>
<li>Simultaneous Determination of Pesticide Residues in Vegetable Extract by LC/MS/MS (LCMS™-8050) | 2020 | Shimadzu
</li>
<li>Determination of Pesticides and Mycotoxins in Cannabis Flower as Defined by Legalized U.S. State Recreational Cannabis Regulations | 2020 | Agilent Technologies
</li>
<li>Using pH as a Method Development Tool with Agilent InfinityLab Poroshell 120 CS-C18 | 2020 | Agilent Technologies
</li>
<li>Determination of Pesticides and Mycotoxins as Defined by California State Recreational Cannabis Regulations | 2020 | Agilent Technologies
</li>
<li>Direct Analysis of Glyphosate, Glufosinate and AMPA in Foods Using a Triple Quadrupole LC/MS/MS | 2020 | Shimadzu
</li>
<li>New Strategy for Glyphosate, Glufosinate, and AMPA quantification in food: In-vial addition of pairing agent | 2021 | Shimadzu
</li>
<li>Simultaneous quantification of aminoglycoside antibiotics in milk using the SCIEX Triple Quad™ 3500 LC-MS/MS System | 2020 | SCIEX
</li>
<li>Analysis of Captan, Folpet, and their Derivatives in Food with APCI-LCMS™-8060 | 2020 | Shimadzu
</li>
<li>The Analysis of Sudan and other Azo Dyes in Spices Using Liquid Chromatography - Tandem Quadrupole Mass Spectrometry | 2021 | Waters
</li>
<li>Quick Screening and Quantitative Analysis of Carcinogens in Medicinal Plants Using DPiMS™-8060 | 2020 | Shimadzu
</li>
<li>Analysis of Mycotoxins in Cannabis Plant Material and Derivative Products by Immunoaffinity Enrichment LC-MS/MS | 2021 | Waters
</li>
<li>LC-MS/MS Method Development and Validation for the Quantitative Determination of Regulated Mycotoxins in Cereal Grain Flours Using Simplified Sample Preparation Conditions on Xevo TQ-XS | 2020 | Waters
</li>
</ul>
<h4>Posters (13)</h4>
<ul>
<li>Analysis of the California list of Pesticides and Mycotoxins in Edibles | 2020 | Restek
</li>
<li>Analytical Solutions for Food Safety – a Closer Look in the Analytical Toolbox | 2020 | Shimadzu
</li>
<li>California and Oregon’s Complete Residual Pesticide Analysis using a Shimadzu LCMS-8060 | 2020 | Shimadzu
</li>
<li>Efficient introduction of ionic compounds into LC-MS using the improved ESI unit; Application to dirty samples | 2020 | Shimadzu
</li>
<li>Detection of typical GCMS Pesticides in Cannabis Matrix utilizing APCI-LCMS | 2020 | Shimadzu
</li>
<li>Quantification of Glyphosate, Glufosinate, and AMPA in Food via In-vial Addition of Pairing Agent | 2020 | Shimadzu
</li>
<li>ROUTINE DETERMINATION OF HIGHLY POLAR CATIONIC PESTICIDES AND PLANT GROWTH REGULATORS BY LC-MS/MS | 2020 | Waters
</li>
<li>EVALUATING SYSTEM PERFORMANCE DURING ROUTINE ANALYSIS OF FOOD COMMODITIES FOR PESTICIDE RESIDUES | 2020 | Waters
</li>
<li>EXPLORING THE ANALYTICAL SCOPE OF PESTICIDES, IONIZABLE IN A SINGLE INJECTION BY LC-MS/MS | 2020 | Waters
</li>
<li>Agilent ASMS 2020 Posters Book | 2020 | Agilent Technologies
</li>
<li>Direct Analysis of Glyphosate, Glufosinate and AMPA in Foods Using a Triple Quadrupole LC/MS/MS | 2020 | Shimadzu
</li>
<li>Analysis of Captan, Folpet and their derivatives in food with APCI-LCMS-8060 | 2020 | Shimadzu
</li>
<li>Highly sensitive direct analysis of glyphosate, glufosinate and AMPA in the beverages by LC-MS/MS | 2020 | Shimadzu
</li>
</ul>
<h4>Guides (5)</h4>
<ul>
<li>Confidently Analyze Cannabis Flower and Hemp - Application compendium for cannabis potency and safety | 2020 | Agilent Technologies
</li>
<li>Solutions for Flexible Work Styles LCMS Food Safety Applications | 2020 | Shimadzu
</li>
<li>Waters Application Notes - Food Testing | 2020 | Waters
</li>
<li>Waters ACQUITY UPC2 Bibliography - A compendium of references to scientific journal articles based on the technique of UltraPerformance Convergence Chromatography (UPC2) | 2020 | Waters
</li>
<li>Food integrity application compendium | 2020 | Thermo Fischer Scientific
</li>
</ul>
<h3>Environmental</h3>
<h4>Applications (5)</h4>
<ul>
<li>Determination of Anionic Polar Pesticides in High Water Foodstuffs | 2020 | Waters
</li>
<li>Measurement of Underivatized Glyphosate and Other Polar Pesticides in Surface and Drinking Water | 2021 | Agilent Technologies
</li>
<li>Determination of Oxyanions in Drinking Water by Direct Injection Using Liquid Chromatography-Tandem Quadrupole Mass Spectrometry | 2021 | Waters
</li>
<li>Analysis of Captan, Folpet and Their Derivatives in Water by APCI-LCMS™-8060 | 2020 | Shimadzu
</li>
<li>An Alternative Ionization Technique for Perfluorinated Alkyl Substance (PFAS) Analysis: Evaluating UniSpray for Water and Soil Samples | 2020 | Waters
</li>
</ul>
<h4>Posters (2)</h4>
<ul>
<li>Trace analysis of abused drugs in waste water sample by AOE system coupled with LCMS-8060 | 2020 | Shimadzu
</li>
<li>Towards Single System for Total Water Analysis. LC-MS/MS screening of 325 PPCP Contaminants in Tap and Surface Water | 2020 | Shimadzu
</li>
</ul>
<h4>Presentations (1)</h4>
<ul>
<li>Measurement of Underivatized Glyphosate and Other Polar Pesticides in Aqueous Matrices Using LC-TQ | 2020 | Agilent Technologies</li>
</ul>
<h4>Guides (1)</h4>
<ul>
<li>Waters ACQUITY UPC2 Bibliography - A compendium of references to scientific journal articles based on the technique of UltraPerformance Convergence Chromatography (UPC2) | 2020 | Waters</li>
</ul>

Overview of LC/QQQ applications of pesticide analysis - Food and Environment (2020-2021)

There is a huge amount of information available today and finding the right ones quickly, or for a specific technique or combination of techniques is often a time consuming matter. The LabRulez database will significantly speed up and facilitate your search, especially in commercial sources.
We bring you an overview of currently available 26 webinars focused on GPC/SEC technology and its connection with various detectors, GPC/SEC column selection and a number of unique applications such as separation Biomolecules, Polymers, AAV, Protein or classic Pharmaceutical applications.
Finally, selection of the latest GPC/SEC applications in the [LabRulezLCMS library] (https://lcms.labrulez.com/library?instrumentationItems=42) of which more than 300 are currently available. 
<blockquote>
<h4><a href="https://lcms.labrulez.com/webinars?tags=35">👉 DETAILS AND REGISTRATION IN THE GPC/SEC WEBINARS SECTION</a></h4>
</blockquote>
<blockquote>
👉 Search and filter in more than 1 500 GC, LC and MS webinars.
</blockquote>
<h5>1. Agilent Technologies: SEC and IEX for Biomolecules: Column Selection and Troubleshooting (Th, 28.4.2022, 17:00 (CEST - Prague))</h5>
Size Exclusion and Ion Exchange are typically run in non-denaturing conditions. That’s one of the reasons they are the two most common types of HPLC used for biomolecules.
<h5>2. Agilent Technologies: ChromTalk Episode 10: Concern for Compound Isolation or Purification? (RECORD | Already taken place Th, 6.1.2022)</h5>
We shall discuss in details about the fundamentals behind purifications, selection of parameters to get the best yield, choice of columns &amp; system solutions &amp; automation for throughput requirements.
<h5>3. Agilent Technologies: ChromTalk Episode 9: Gel Permeation/ Size Exclusion Chromatography (RECORD | Already taken place Tu, 21.12.2021)</h5>
In this Agilent ChromTalk, you will go back to basics of all about GPC/SEC, its application area, selection of GPC/ SEC columns &amp; method conditions, typical types of GPC/ SEC instruments from experts.
<h5>4. Tosoh Bioscience: PolyAnalysis Forum - New Polymers with Complex Architectures (RECORD | Already taken place Th, 2.12.2021)</h5>
PolyAnalysis Forum - New polymers with complex architectures: why and how?
<h5>5. Thermo Fisher Scientific: Innovation Summit - Advancing Analytical Science - Day 2 (RECORD | Already taken place We, 20.10.2021)</h5>
Are you a new or existing separation scientist or chromatographer in industry and academia? Today we will discuss topics such as advances in large biomolecules and Chromatography with advanced detection.
<h5>6. The Analytical Scientist/Wyatt Technology: Measuring AAV Quality Attributes with Light Scattering (RECORD | Already taken place Tu, 19.10.2021)</h5>
We will present highly precise SEC-MALS method utilizes multi-angle light scattering in line with standard size-exclusion chromatography, requiring little sample prep, no reagents and minimal operator effort.
<h5>7. SelectScience/Phenomenex: Size exclusion native mass spectrometry for the characterization of monoclonal antibodies (RECORD | Already taken place We, 6.10.2021)</h5>
In this SelectScience webinar, Brian Rivera will discuss how non-denaturing SEC Native MS can be leveraged to streamline mAb characterization.
<h5>8. The Analytical Scientist: The Extreme HPLC Technology Forum (RECORD | Already taken place Th, 30.9.2021)</h5>
Sharing innovation updates, applications, and words of wisdom, our four technology providers will take you to the extreme edges of HPLC in terms of accuracy, robustness, and sample size.
<h5>9. Agilent Technologies: Protein A Affinity Capture followed By AdvanceBio SEC Aggregation Analysis using the Agilent InfinityLab Series 2D-LC System (RECORD | Already taken place We, 29.9.2021)</h5>
Protein biopharmaceuticals such as recombinant monoclonal antibodies are currently in widespread use for the treatment of various life-threatening diseases including cancer and autoimmune diseases.
<h5>10. Phenomenex: Method Robustness and Transferability for Modern Size Exclusion Methods (RECORD | Already taken place Tu, 24.8.2021)</h5>
Understand the impact of HPLC/UHPLC systems on obtaining robust and reproducible aggregate analysis data by SEC and determine the experimental parameters to vary to ensure analytical method robustness.
<h5>11. Phenomenex: Size Exclusion Chromatography: How to Select an SEC Column and Optimize a Method (RECORD | Already taken place Tu, 24.8.2021)</h5>
Accurate characterization of monoclonal antibody (mAb) aggregates has huge significance in the development of new therapeutic agents. Selection of SEC column and optimizing method place a crucial role.
<h5>12. Agilent Technologies: Structural Characterization of Biopolymers by GPC/SEC Coupled to Special Detectors (ELSD, Viscometer, Light Scattering) (RECORD | Already taken place We, 11.8.2021)</h5>
This webinar explores a basic introduction regarding biopolymers and a detailed discussion on characterization techniques via GPC/SEC, as well as the potential of adding advanced detection technologies.
<h5>13. Agilent Technologies: Superior results need superior columns…Make the right choice for your GPC Application (RECORD | Already taken place We, 28.7.2021)</h5>
Join us in this live webinar to discuss on various aspects of GPC column Selection.
<h5>14. Agilent Technologies: High Resolution Size Exclusion Chromatography (SEC) Analysis of Intact Protein Biotherapeutics (RECORD | Already taken place Mo, 5.7.2021)</h5>
This presentation will highlight comprehensive workflows for the analytical characterization of proteins using SEC, ProA-SEC 2D-LC and SEC-MS.
<h5>15. Agilent Technologies: Helpful ‘hints’ for Protein SEC Success (RECORD | Already taken place Th, 27.5.2021)</h5>
This webinar will provide key guidelines for successful SEC method development.
<h5>16. Waters Corporation: Waters LC Symposium - Materials and Pharma (RECORD | Already taken place Th, 25.3.2021)</h5>
Waters Corporation is pleased to bring together chromatography thought leaders for a week of scientific talks, demonstrations, discussions, and networking regarding novel LC technologies and applications.
<h5>17. Agilent Technologies: GPC Analysis with Fraction collection using OpenLAB CDS: A practical approach to simplify further characterization of complex mixtures (RECORD | Already taken place We, 24.3.2021)</h5>
This webinar will present a complete solution for an Agilent 1260 Infinity II GPC system coupled to a Fraction Collector controlled by Open LAB CDS software with integrated GPC workflows.
<h5>18. SelectScience/Waters Corporation: Taming the Viral Beast: Applying LC and LC-MS Technologies to the Analysis of rAAV Viral Vectors (RECORD | Already taken place We, 16.12.2020)</h5>
In this webinar you will discover the challenges associated with analyzing AAV samples, and discover how LC and LC-MS workflows can help solve these setbacks, including those that employ SEC, AEX.
<h5>19. Separation Science: Advanced Technologies Combined for Enhanced GPC/SEC Analysis of Polymers (RECORD | Already taken place Th, 29.10.2020)</h5>
Separation Science, in collaboration with Tosoh Bioscience offers an webinar that describes the latest technologies in GPC/SEC to characterize the molecular weight distribution and architecture of polymers.
<h3>Selection of the latest GPC/SEC applications in <a href="https://lcms.labrulez.com/library?instrumentationItems=42">LabRulezLCMS library</a></h3>
<ul>
<li>Method Optimization for the Analysis of Monoclonal Antibodies by Size-Exclusion Chromatography (Technical notes | 2022 | Shimadzu)
</li>
<li>Reliable High Resolution Protein SEC Separations for Online Native LC-MS mAb Analysis (Applications | 2022 | Waters)
</li>
<li>USP Monograph mAb SEC Method Robustness on the XBridge Premier Protein SEC Column (Applications | 2022 | Waters)
</li>
<li>Modern Size-Exclusion Chromatography Separations of Biosimilar Antibodies at Physiological pH and Ionic Strength (Applications | 2022 | Waters)
</li>
<li>Waters ACQUITY and XBridge Premier Protein SEC 250 Å Columns: A New Benchmark in Inert SEC Column Design (Applications | 2022 | Waters)
</li>
<li>Analysis of protein drugs aggregation Using Size Exclusion Chromatography (Applications | 2022 | Shimadzu)
</li>
<li>Solutions for Aggregate and Fragment Analyses - SEC COLUMN TECHNOLOGY (Brochures and specifications | 2022 | Waters)
</li>
<li>Rapid AAV Concentration Determination Using Size-Exclusion Chromatography with Fluorescence and UV Dual DetectionMS (Applications | 2021 | Waters)
</li>
<li>Best Practices for Maintaining Column Performance in Size-Exclusion Chromatography during Long-Term StorageMS (Technical notes | 2021 | Waters)
</li>
<li>Evaluation of SEC Columns for Analysis of ADC Aggregates and FragmentsMS (Applications | 2021 | Agilent Technologies)
</li>
<li>High-Performance Biopharma Analysis - Application compendiumMS (Guides | 2021 | Agilent Technologies)
</li>
<li>Span the Spectrum of Bio LC - Agilent InfinityLab Bio LC SolutionsMS (Brochures and specifications | 2021 | Agilent Technologies)
</li>
<li>Watrex DeltaGel GPC/SEC kolonyMS (Brochures and specifications | 2021 | Watrex Praha)
</li>
<li>Agilent AdvanceBio SEC 2.7 μm Columns - User guideMS (Manuals | 2021 | Agilent Technologies)
</li>
<li>Agilent Bio SEC-3 LC ColumnsMS (Brochures and specifications | 2021 | Agilent Technologies)
</li>
<li>Agilent Bio SEC-5 LC ColumnsMS (Brochures and specifications | 2021 | Agilent Technologies)
</li>
<li>Agilent AdvanceBio SEC 1.9 μm Columns - User guideMS (Manuals | 2021 | Agilent Technologies)
</li>
<li>Helpful ‘Hints’ for Protein SEC SuccessMS (Presentations | 2021 | Agilent Technologies)
</li>
<li>Analysis of Polymer Additives - Shim-pack GPC 805 + GPC 801MS (Applications | 2021 | Shimadzu)
</li>
<li>Calibration Curves of Shim-pack DiolMS (Applications | 2021 | Shimadzu)
</li>
<li>Seamless Process from Protein Purification to EvaluationMS (Applications | 2021 | Shimadzu)
</li>
<li>Analysis of Carboxymethyl Cellulose Using an Integrated HPLC SystemMS (Applications | 2021 | Shimadzu)
</li>
<li>Resolution of Adeno-Associated Viral Vector Aggregates and Fragments with Agilent Bio SEC-5MS (Applications | 2021 | Agilent Technologies)
</li>
<li>Automated Monoclonal Antibody Subunit Analysis by Online Reduction Using 2D-LC/MSMS (Applications | 2021 | Agilent Technologies)
</li>
<li>ASMS 2021: Rapid Desalting of Proteins for Direct Infusion Intact Mass Analysis using an Optimized SEC TipMS (Posters | 2021 | Waters)
</li>
<li>Advanced Polymer Chromatography Reverse Phase Method for Polymer Additives Using Tetrahydrofuran and Evaporative Light Scattering Detection in Under Three Minutes per SampleMS (Applications | 2021 | Waters)
</li>
<li>Determination of Multiple Attributes of Monoclonal AntibodiesMS (Applications | 2021 | Agilent Technologies)
</li>
<li>The Agilent InfinityLab 2D-LC ProtA‑SEC Kit and ServiceMS (Brochures and specifications | 2021 | Agilent Technologies)
</li>
<li>Method Transfer of Size-Exclusion and Ion-Exchange Methods for Monoclonal Antibody Analysis from HPLC to the Arc Premier SystemMS (Applications | 2021 | Waters)
</li>
<li>Evaluating the Waters ACQUITY Premier System as a Flexible LC Platform That Can Be Broadly Deployed in Biopharmaceutical LabsMS (Applications | 2021 | Waters)
</li>
<li>Demonstrating a Fit-for-Purpose Solution for Biopharmaceutical Separations with the ACQUITY UPLC H-Class PLUS Bio Binary SystemMS (Applications | 2021 | Waters)
</li>
<li>Agilent Bio SEC-5 for Analysis of Virus-Like Particles (VLP)MS (Applications | 2021 | Agilent Technologies)
</li>
<li>Analyzing Monoclonal Antibody (mAb) Fragments and Dimers Using Size Exclusion Chromatography (SEC)MS (Other | 2021 | Agilent Technologies)
</li>
<li>Analyzing Monoclonal Antibody (mAbs) and mAb-Derived Therapeutics Using Native SEC-MSMS (Applications | 2021 | Agilent Technologies)
</li>
<li>Arc HPLC Aqueous SEC/GPC Separation of PEO/PEGMS (Applications | 2021 | Waters)
</li>
<li>SEC/GPC, DSC, and TGA Analysis of Low Molecular Weight Poly-L-lactic AcidMS (Applications | 2021 | Waters)
</li>
<li>Characterization of Antibody‑Drug Conjugates Using 2D-LC and Native MS (Applications | 2021 | Agilent Technologies)
</li>
</ul>

Gel Permeation (GPC) / Size Exclusion Chromatography (SEC) Webinars

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 1st July 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!
<blockquote>
<h4>👉 <a href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></h4>
</blockquote>
<blockquote>
👉 Need info about different analytical techniques? Peek into <a href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.
</blockquote>
<h4>1. Thermo Fisher Scientific: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_002748_ic_combustion_ic_system_environmental_an002748_na_en_a379c1e9d2.pdf">Screening of PFAS compounds in wastewater using adsorbable organic fluorine with combustion ion chromatography (CIC)</a></h4>
<ul>
<li>Application note
</li>
<li>Results from U.S. EPA draft Method 1621 Collaboration Study
</li>
<li>Keywords: EPA Method 1621, AOF, CIC, PFOA, PFHxS, GAC, Dionex, IonPac AS24 column, PFAS, (per- and polyfluoroalkyl substances), combustion ion chromatography (CIC), adsorbable organic fluorine (AOF), granular activated carbon (GAC)
</li>
</ul>
Conclusion: This application note demonstrated our results from the multi-laboratory collaboration study of EPA draft Method 1621. This method shows that CIC is an excellent tool to measure AOF andscreen for PFAS in wastewater. Along with its ease of use, RFIC provides more accurate results as fluoride is well separated from the water dip, ensuring optimized automated peak integration. The method is sensitive, with MBs of 1 ng/mL and MDLs of 2.5 ng/mL, and accurate, with recoveries of 80–120%. Together, these data highlight the power of CIC in eliminating the sample matrix and measuring only the adsorbable organically bound fluorine content in samples, successfully achieving the goals outlined in EPA draft Method 1621.
<h4>2. Metrohm: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_cic_035_download_032bfd1511.pdf">Halogens and sulfur in solid samples according to EN 17813</a></h4>
<ul>
<li>Application note</li>
<li>Rapid analysis with combustion ion chromatography (CIC)</li>
</ul>
Conclusion: Combustion ion chromatography is a straightforward analytical technique to determine halogens and sulfur in environmental and solid matrices. The ceramic setup is especially suitable for CIC analysis of sample matrices with high amounts of alkali metals and/or alkaline earth metals. With the ceramic setup, the robustness of the analysis and the lifetime of consumables is drastically improved. The ease-of-use is further advanced by the possibility to inject different sample volumes – depending on the analyte concentration in the samples – to guarantee that these fit into the calibration range.
Overall, users of this whole validated procedure profit from easy and standardized handling, the precise determination of the analytes, automatic eluent roduction, calibration, and results calculation, low maintenance, and a single-manufacturer setup.
<h4>3. Waters Corporation: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720008215_en_4dd8a24507.pdf">Cyclic™ Ion Mobility Unravels Sites of Chemically Induced Deamidation</a></h4>
<ul>
<li>Application note</li>
</ul>
Abstract: Acid deamidation can mimic the action of tissue transglutaminase in deamidation of specific peptide glutamine residues, and possibly increase the immunotoxicity of the deamidated gluten food ingredient and convey an increased risk to those with coeliac disease. Peptides with multiple deamidation sites may co-elute under the same chromatographic peak and therefore other methods of site determination are required such as ion mobility mass spectrometry, increasing confidence in profiling these post-translational modifications (PTMs).
<h4>4. Waters Corporation: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720008330_en_3f714eaa91.pdf">Sample Preparation and Analysis Workflow to Address the PFAS Primary National Drinking Water Regulation</a></h4>
<ul>
<li>Application note</li>
</ul>
Abstract: The newly announced PFAS National Primary Drinking Water Regulation has set enforceable levels of six PFAS compounds in drinking water for the first time in the United States. Regulatory levels of individual compounds range from 4–10 ng/L (or ppt). The Waters™ PFAS Solution for sample preparation through sample analysis is presented to make implementation of the new PFAS regulations straightforward and accurately reach far below the lowest regulated Maximum Contamination Level of 4 ng/L.
<h4>5. Agilent Technologies: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_catecholamine_ultivo_poroshell_5994_7446en_agilent_e7e1512a7d.pdf">Determination of Catecholamines by Agilent Ultivo LC/TQ with an InfinityLab Poroshell 120 Aq‑C18 Column</a></h4>
<ul>
<li>Application note - Clinical Research</li>
</ul>
Abstract: In this application note, a quantification method was established for catecholamines (3-MT, DA, E, NE, MN, and NMN) using the Agilent Ultivo liquid chromatography/triple quadrupole mass spectrometer (LC/TQ) system with a newly developed Agilent InfinityLab Poroshell 120 Aq-C18 column. The Poroshell 120 Aq-C18 column shows good performance with baseline separation of these six catecholamine compounds. The validation test results give a wide linearity range of 5 to 500 pg/mL in bovine serum albumin (BSA) matrices with excellent accuracy.
<h4>6. Shimadzu: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_ssi_lcms_156_en_671b194ea2.pdf">Oligonucleotide Characterization for Quality Control on the Shimadzu Single Quad Mass Spectrometer LCMS-2050</a></h4>
<ul>
<li>Application News</li>
</ul>
<h5>User Benefits:</h5>
<ul>
<li>The Shimadzu LCMS-2050 single quadrupole mass spectrometer and SPD-M30A photodiode array detector offer a straightforward workflow for quantitating oligonucleotides, streamlining the analysis process.</li>
<li>Confident mass confirmation can be obtained over a wide mass range of nucleotide oligomers.</li>
<li>The Shimadzu LCMS-2050 enables the detection and identification of lower levels of impurities commonly found in synthetic oligomers.</li>
</ul>
<h4>7. Shimadzu: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/c197_e004_1a77445226.pdf">Ion Chromatography Solutions for Environmental Analysis</a></h4>
<ul>
<li>Guide
</li>
<li>Overview of Environment Analysis
</li>
<li>Relevance of Ion Chromatography in Environmental Analysis
</li>
<li>Overview of Ion Chromatography
</li>
<li>Introduction of Ion Exchange Chromatography
</li>
<li>Shimadzu’s Ion Chromatography Solutions
</li>
<li>LabSolutions Software
</li>
<li>Lineup of Columns
</li>
</ul>

News from LabRulezLCMS Library Week 27, 2024

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 29th July 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!
<blockquote>
<h4>👉 <a href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></h4>
</blockquote>
<blockquote>
👉 Need info about different analytical techniques? Peek into <a href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.
</blockquote>
This week we bring to you applications, brochures and specifications from Agilent Technologies, Shimadzu, Metrohm, Thermo Fisher Scientific, and Waters Corporation!
<blockquote>
💡 Almost 1000 ASMS posters and presentations <a href="https://lcms.labrulez.com/library?mainauthorItems=284">available in the LabRulezLCMS library</a> (unique search and filtering)
</blockquote>
<h4>1. Metrohm: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_v_240_download_23ff0d3438.pdf">Determination of total iodine in thyroid tablets with polarography</a></h4>
<ul>
<li>Application Note</li>
</ul>
Polarography in pharmaceutical analysis: indirect determination of iodine after dry ashing in a muffle furnace
Thyroid hormones are made by the thyroid gland and play an important role in regulating metabolism and growth. Iodine acts as a building block and the specific number of iodine atoms determines the type of the hormone: four for thyroxine (T4) and three for triiodothyronine (T3). The number of iodine atoms is critical for the functionality of thyroid hormones. Levothyroxine and liothyronine (synthetic forms of thyroid hormones T4 and T3) are essential components of thyroid tablets. T4 is less active and needs to convert to the more active T3 to be fully effective. Accurate iodine determination in thyroid tablets is a crucial quality control measure, ensuring the effectiveness and safety of thyroid treatments. A robust method is introduced for indirect determination of total iodine content in thyroid tablets as iodate, according to United States Pharmacopeia (USP) guidelines using the 884 Professional VA and the Multi-Mode Electrode pro.
<h4>2. Shimadzu: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00749_en_efbfc8887d.pdf">Efficient Preparative Purification Workflow of Synthetic Peptide Using Analytical/Preparative Switching LC-MS System</a></h4>
<ul>
<li>Application Note</li>
</ul>
User Benefits
<ul>
<li>A multi-step seamless preparative purification workflow of synthetic peptides can be performed within a single LC-MS system setup.</li>
<li>LabSolutions MD can provide easy investigation for optimal separation conditions for target compounds.</li>
<li>Target compounds can be purified from crude samples with high purity and high recovery based on the excellent identification capability of LCMS-2050.</li>
</ul>
Introduction: In the 2000s, biopharmaceuticals such as antibody drugs have emerged but there are many challenges to overcome because of their manufacturing process using genetic technology. Consequently, middle molecule drugs are paid attention, peptide therapeutics, one of middle molecule drugs which have advantages of being manufactured at low cost, easily taken into cells because of theirsmall molecular weight, and prevented from degradation by adopting a specific three-dimensional structure when they are taken into human body. Since such peptides are produced by chemical synthesis like small molecule drugs, it is essential to purify, fractionate, and confirm the purity of the final synthesized product. In this article, we present a case study of multi-step seamless preparative purification workflow (optimizing separation conditions, scaling-up, fractionation, and confirming (purity/recovery) of a peptide using preparative purification liquid chromatograph Nexera Prep (Fig. 1) based on Application News <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00650_en_f326c9720e/an_01-00650-en.pdf">01-00650-EN</a> and <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00651_en_d88902f27d/an_01-00651-en.pdf">01-00651-EN.</a>
Conclusion: The seamless preparative purification workflow can be executed using analytical/preparative switching LC-MS system. In addition, LabSolutions MD can automatically create an analytical batch schedule in which various HPLC parametersettings are examined, resulting in efficient optimization ofseparation conditions. Furthermore, LCMS-2050 enables highly selective fractionation of target compounds based on MS trigger. The analytical/preparative switching LC-MS used in this article has both analytical and preparative flow paths, enabling an efficient preparative purification workflow in the preparative purification of peptide including itssynthesis confirmation process.
<Related Applications>

<ol>
<li>Seamless Purification Workflow from Analytical to Preparative in Single LC-MS System, <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00650_en_f326c9720e/an_01-00650-en.pdf">01-00650-EN</a></li>
<li>High Purity Preparative Purification Enabled by UV/MS Trigger on LC-MS System,<a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00651_en_d88902f27d/an_01-00651-en.pdf">01-00651-EN.</a></li>
</ol>
<h4>3. Shimadzu: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/65237_tn_sfc_infographic_a417cd5f28.pdf">Rewriting the Book on Supercritical Fluid Chromatography</a></h4>
<ul>
<li>Technical Note</li>
</ul>
Fluids become supercritical when they are highly compressed and take on the properties of both a liquid and a gas. Supercritical carbon dioxide (CO₂) can be used as the mobile phase in supercritical fluid chromatography (SFC). Until recently, this technique had been overlooked in favor of high-performance liquid chromatography (HPLC) and gas chromatography (GC). However, new developments in the field have demonstrated that SFC can be a highly cost-effective, accurate and sensitive solution capable of outperforming HPLC and GC. In light of these new technologies, it’s time to reshape the field of chromatography with SFC.
<h4>4. Agilent Technologies: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_size_exclusion_chromatography_1290_infinity_ii_bio_5994_7664en_agilent_585512a949.pdf">Advanced SEC-MALS Analysis of Monoclonal Antibodies with the Agilent 1260 Infinity II Multi-Angle Light Scattering Detector</a></h4>
<ul>
<li>Application Note</li>
</ul>
Abstract: Size exclusion chromatography (SEC) in combination with multiangle light scattering (MALS) detection enables accurate and reliable separation and molecular weight (MW) determination of monomer and aggregate protein species. The Agilent 1260 Infinity II Multi-Angle Light Scattering detector coupled to the Agilent 1290 Infinity II Bio LC System offers the possibility to measure the intensity of the scattered light at multiple angles to gain more precise absolute MW for the analysis of biopharmaceuticals. The 1290 Infinity II Bio LC is specially designed for conditions used in biochromatography with a completely iron-free flow path and a high tolerance to salt-based buffers such as those used in SEC.
Conclusion: The possibility of using a MALS detector with 20 angles enables highly precise calculations of absolute molar mass. The MALS detector shows higher sensitivity than UV detection especially for higher protein aggregates, adding valuable information to a conventional SEC setup. The smaller angles allow the determination of higher molecular mass aggregates with high sensitivity and precision. The excellent resolution of the Agilent AdvanceBio SEC 300 Å column enables clear separation of monomer, dimer, higher aggregates, and mAb fragments, leading to unique MW determinations for the separated peaks. The combination of the Agilent 1290 Infinity II Bio LC System with the Agilent 1260 Infinity II Multi-Angle Light Scattering detector provides a complete biocompatible flow path to ensure trusted results for challenging solvent conditions or iron-reactive samples.
<h4>5. Agilent Technologies: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/te_evaluation_rohs_compliant_ion_injectors_5994_7613en_agilent_1dfe200fad.pdf">Evaluation of Agilent RoHS-Compliant LC/MS Ion Injectors</a></h4>
<ul>
<li>Technical Note</li>
</ul>
Introduction: Recently, governments across the globe have started to reduce the use of hazardous materials in electronic components. Specifically, the Restriction of Hazardous Substances in Electrical and Electronic Equipment (RoHS) Directive in the European Union has been driving this effort. The Agilent resistive ion injector has been updated to a leaded glass–free, RoHS-compliant-without-exemption part for use in Agilent LC/MS quadrupole instruments that do not have an ion funnel. The ion injector acts to pull desolvated ions from the desolvation chamber into the MS (Figure 1). In doing so, the ions are culminated into a single, narrow beam, allowing the MS to separate the ions according to their mass-to-charge ratio (m/z). The ion injector is resistive, which allows for fast polarity mode switching, and has metal contacts at either end for electrical connection to the MS. In this technical overview, the performance of the Agilent RoHS ion injectors was compared to that of their respective legacy ion injector models (Table 1).
Conclusion: In this technical overview, the Agilent RoHS-compliant ion injectors were compared to their respective Agilent legacy ion injectors. The studies presented in this technical overview demonstrate equivalent functionality between the legacy and RoHS ion injector models. Specifically, tune performance was shown to be equivalent between RoHS and legacy ion injectors, and lifetime performance under acidic and basic conditions was also shown to be comparable between RoHS and legacy ion injections, including at high mass on the Agilent 6545XT AdvanceBio LC/Q-TOF. Moreover, IDLs and S/N were within specifications on multiple of the tested RoHS ion injectors. Furthermore, the RoHS ion injectors held up to 10,000 injections of bovine urine and maintained tune criteria throughout, demonstrating excellent robustness. Finally, no differences in performance were observed on two different MS platforms when running target pharmaceuticals, both in neat samples and diluted human urine. In conclusion, the RoHS Agilent FS and Agilent Ultivo and MSD iQ ion injectors are suitable drop-in replacements for the legacy Agilent FS and Agilent Ultivo ion injectors.
<h4>5. Waters Corporation: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/2024_pegs_hanna_peptidworkskit_ce7f104856.pdf">PEGS: SIMPLE AND AUTOMATABLE SAMPLE PREPARATION FOR TRYPTIC PEPTIDE MAPPING WITH THE PEPTIDEWORKS KIT</a></h4>
<ul>
<li>Poster, PEGS 2024</li>
</ul>
NTRODUCTION
Reliable day-to-day sample preparation is a critical factor in generating an effective peptide mapping method. Sample preparation for peptide mapping can result in method-induced peptide modifications, over- or under-digestion of the protein, and autolysis of the proteolytic enzyme, each of which complicate data analysis and interpretation.
Waters’ PeptideWorkTryptic Protein Digestion Kits deliver fast and reliable sample preparation for routine peptide mapping of therapeutic proteins. The sample preparation kit is centered around RapiZymTrypsin, Waters’ homogeneously methylated, recombinant trypsin. RapiZyme Trypsin enables speed, digestion fidelity, and low levels of trypsin- derived peptides through its autolysis resistance, purity, and high activity. RapiZyme Trypsin can be used at high concentrations to achieve faster digestions without requiring high temperatures or sacrificing digestion completeness.
CONCLUSION
<ul>
<li>Comprehensive kit and protocol for preparation of tryptic protein digests </li>
<li>Automatable preparation of 24 samples in under 2.5 hours</li>
<li>Reproducible sample prep without sacrificing digestion completion or generating high levels of method-induced peptide modifications </li>
<li>93% reduction in missed cleavages and 55% reduction in non-specific cleavages compared to a leading immobilized trypsin digest kit</li>
<li>Suitable for peptide mapping of ADCs</li>
</ul>
<h4>6. Thermo Fisher Scientific: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_002998_ic_ms_integrion_isq_ec_environmental_water_an002998_na_en_b7542bd84c.pdf">Determination of selenite and selenate in environmental waters by ion chromatography-mass spectrometry (IC-MS)</a></h4>
<ul>
<li>Application note</li>
</ul>
Keywords: Single quadrupole mass spectrometer, Dionex Integrion HPIC, Dionex IonPac AS11-HC column, ISQ-EC, ADRS 600 suppressor
Goal: To develop a method to determine selenite and selenate in spiked environmental water by coupling ion chromatography (IC) with single quadrupole mass spectrometry.
Conclusion: An IC-MS method that enables the determination of selenium species in simulated contaminated environmental waters was developed. The reagent-free ion chromatography system provides excellent reproducibility, yielding exceptional quantification accuracy and consistently reliable results. Recoveries for selenite and selenate in the three water samples ranged between 90–110%, with a method LOD of 4 and 2 µg/L for selenite and selenate, respectively. This method is helpful for highly selective screening of selenium species at µg/L levels in environmental waters. Although not with the same sensitivity as IC-ICP-MS, it offers the advantage of selectively identifying selenium species through their m/z ratio. Moreover, this method enables determining whether drinking water exceeds the EPA&#39;s outlined MCL of 50 ppb for selenium contamination.
<h4>7. Thermo Fisher Scientific: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_002998_ic_ms_integrion_isq_ec_environmental_water_an002998_na_en_b7542bd84c.pdf">Size-exclusion chromatography of adeno-associated viruses with the SurePac Bio 550 SEC MDi column</a></h4>
<ul>
<li>Application note</li>
</ul>
Keywords: Biopharmaceutical, size-exclusion liquid chromatography, SEC, adeno-associated virus, AAV, monomeric capsids, high molecular weight species, HMWS, 3 µm SurePac Bio 550 SEC MDi column, monodisperse
Key benefits
<ul>
<li>High-resolution separation of monomeric AAV and aggregates by size-exclusion liquid chromatography within 4 minutes</li>
<li>Utilizing UV and fluorescence detection for easy and conclusive confirmation of peak identities</li>
<li>Resolution of aggregate and monomer peaks preserved even for injection of large volumes</li>
<li>Consistent lot-to-lot and column-to-column performance</li>
</ul>
Goal: To demonstrate baseline separation of adeno-associated virus (AAV) monomeric capsids and high molecular weight species (HMWS) using a 4.6 × 150 mm sizeexclusion liquid chromatography column packed with 3 µm monodisperse silica particles with a 550 Å pore size.
Conclusions
<ul>
<li>The SurePac Bio 550 SEC MDi column delivers baseline resolution of AAV monomer and aggregate peaks in less than 4 minutes.</li>
<li>The SurePac Bio 550 SEC MDi column consistently delivers reliable separation of AAV across different lots.</li>
<li>Careful optimization of flow rate, sample loading amounts, and buffer conditions is crucial for achieving accurate and consistent results in SEC AAV separation.</li>
<li>The SurePac Bio 550 SEC MDi column outperforms other commercially available columns in AAV analysis, displaying advantages such as sharper peaks, improved resolution, and lower operational pressure</li>
</ul>

News from LabRulezLCMS Library - Week 31, 2024

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 5th August 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!
<blockquote>
<h4>👉 <a href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></h4>
</blockquote>
<blockquote>
👉 Need info about different analytical techniques? Peek into <a href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.
</blockquote>
This week we bring to you applications, brochures and specifications from Agilent Technologies, Shimadzu, Metrohm, Thermo Fisher Scientific, and Waters Corporation!
<h4>1. Shimadzu: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/IVDR_White_Paper_2024_Shimadzu_737d95b5fb.pdf">How Shimadzu is simplifying your compliance workload for the new IVD regulation!</a></h4>
<ul>
<li>Technical Note</li>
</ul>
What is the New EU In Vitro Diagnostic Regulation (IVDR)?
New In Vitro Diagnostic Medical Device Regulation (IVDR) (EU) 2017/746 was published in EU Official Journal in May 2017 in order to ensure a high level of safety and health whilst supporting innovation. It applies from 26 May 2022 and replaced the previous IVD Directive (98/79/EC). This regulation set rules for in vitro diagnostic devices such as reagent, calibrator, kit, instrument, and software intended by the manufacturer to be used for diagnostic purposes.
IVDR retains some concepts of IVD Directive such as CE marking or essential requirements (under IVDR called “general safety and performance requirements”). As in the past, the manufacturers have to demonstrate that they and their devices meet the requirements through conformity assessment procedure.
Different from IVD Directive, however, IVDR introduced the riskbased classification (Figure 1). Under IVDR devices are classified in four: The devices with highest risks are classified in class D. The class A devices have low risks and include products for general laboratory use, accessories which possess no critical characteristics.
<h4>2. Shimadzu: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/LCMS_157_Determination_of_30_PFAS_Seafood_LCMS_8060_NX_060524_a44e3a68b5.pdf">Determination of 30 PFAS in Seafood by Liquid Chromatography Triple Quadrupole Mass Spectrometry (LC-MS/MS)</a></h4>
<ul>
<li>Application</li>
</ul>
User Benefits
<ul>
<li>Validated method for 30 PFAS in seafood (shrimp) meeting all criteria of AOAC SMPR 2023.003</li>
<li>High precision, excellent recovery, low Limit of Quantification (LOQ)</li>
<li>Simple and rapid extraction using QuEChERS</li>
</ul>
Introduction: Per-and polyfluorinated substances (PFAS) are a diverse group of man-made chemicals used in numerous products since the 1950s. PFAS can enter the food supply by contact in environmentally contaminated areas, during food processing, or exposure to packaging. Because PFAS have been linked to serious health effects, accurate methodology is needed. In this application news, we describe a single laboratory validation study with a rapid extraction of low concentrations of 30 PFAS in shrimp using the QuEChERS technique followed by analysis using the Shimadzu Nexera Liquid Chromatograph coupled to a Shimadzu LCMS-8060NX triple quadrupole mass spectrometer (Figure 1). 
We optimized the chromatography and instrument operating parameters to achieve excellent peak shape, separation, and sensitivity. Sensitivity was improved for 4 PFAS, PFOA, PFHxS, PFNA, and PFOS.
In this study, we spiked samples at three concentrations in triplicate. For greater accuracy, standards were matrixmatched and extracted and spikes were quantified using the isotope dilution technique. Recovery and precision were compared to the requirements of AOAC SMPR 2023.003. In addition, we determined the Limit of Quantitation (LOQ) as the lowest concentration meeting accuracy and precision, ion ratio, retention time, and signal-to-noise ratio criteria of the qualifier ion. All recovery, precision, and LOQ’s met the acceptance criteria of the SMPR. The target analytes, their acronym, chemical abstract number, and experimentally determined LOQ are shown in Table 1.
Conclusion: The Shimadzu LCMS-8060NX Triple Quadrupole Mass Spectrometer coupled with a Shimadzu Nexera Liquid Chromatograph was used in a single laboratory study to measure 30 PFAS compounds in a shrimp matrix and compared to criteria set by AOAC SMPR 2023.003. Chromatography conditions and the mass spectrometer were optimized to achieve excellent separation of all analytes, baseline resolution between linear and branched isomers, and a two-minute separation between PFOS and potentially interfering cholic acids. 
Precision and recovery and the experimentally determined LOQ are well within the requirements of the SMPR.
<h4>3. Agilent Technologies: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/technical_overview_lc_ms_intelligence_5994_7430en_agilent_b00bf96408.pdf">Boost Efficiency and Productivity with Instrument Intelligence and Workflow Automation</a></h4>
<ul>
<li>Technical Note</li>
</ul>
Introduction: Ever-increasing pressure for laboratories to minimize operating costs while maximizing output demands innovative approaches to their daily work. Recent developments in artificial intelligence (AI) and automation can help to alleviate this growing burden. The goal is to get technology to contribute more and more to day-to-day operations by reducing errors and ensuring consistent high-quality data generation. For workflows using Agilent LC/TQ and LC/(Q-)TOF instruments, this goal is becoming a reality.
The latest generation of Agilent LC/TQ and LC/(Q-)TOF instruments offers a suite of intelligent automation features to keep the instruments at peak performance. SWARM autotune allows instruments to be tuned for specific applications and desired performance. Auto- and check tunes can be scheduled to run during downtime so that the instruments are always ready to go. Instrument status is actively monitored using Early Maintenance Feedback (EMF) with constant feedback so that users can anticipate and plan for maintenance. Data are actively examined to ensure high quality. And, if data-related issues are suspected, Intelligent Reflex reinjection workflows automatically work to correct the situation. The culmination of these instrument intelligence features reduces errors, rework, and manual intervention—boosting laboratory efficiency and productivity.
Conclusion: Agilent LC/TQ and LC/(Q-)TOF instruments include numerous intelligence and workflow automation features to boost productivity and efficiency of demanding routine analysis applications. These intelligence features:
<ul>
<li>Maximize instrument performance and analytical sensitivity (application-based SWARM autotune)</li>
<li>Minimize downtime (early maintenance feedback and scheduled tuning)</li>
<li>Maximize sample throughput (intelligent reflex and fast screening workflows) </li>
<li>Ensure that injected samples do not require repeat work(intelligent reflex carryover detection and above calibration range workflows)</li>
<li>Improve sample characterization (intelligent reflex targeted MS/MS confirmation and iterative MS/MS workflows)</li>
</ul>
<h4>4. Agilent Technologies: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_peptide_impurity_two_dimensional_liquid_chromatograhy_5994_7654en_agilent_e71c92f265.pdf">Confirmation of Peptide-Related Impurity Intact Mass Using Agilent 1290 Infinity II Bio 2D-LC and InfinityLab LC/MSD XT</a></h4>
<ul>
<li>Application</li>
</ul>
Abstract: This application note presents the analysis of peptide-related impurities for Semaglutide using an Agilent 1290 Infinity II bio 2D-LC and an Agilent InfinityLab LC/MSD XT system. Peptide-related impurities are challenging to separate from peptide-active pharmaceutical ingredients due to their structural diversity and relatively large molecular weights. To address this challenge, an Agilent AdvanceBio Peptide Plus column, which is stable under high-concentration acidic conditions, was used to achieve good separation of Semaglutide impurities. A solvent system compatible with mass spectrometry (MS) was used with the 1290 Infinity II bio 2D-LC and InfinityLab LC/MSD XT, enabling the acquisition of MS spectra. The deconvolution feature in Agilent OpenLab CDS software, version 2.8, facilitated the easy determination of molecular weights for impurity peaks analyzed by two-dimensional liquid chromatography (2D-LC).
Conclusion: Generally, difficult-to-separate peptide-related impurities can benefit from improved resolution through optimizing the concentration of acidic modifiers and fine-tuning the column performance. However, confirming the molecular weight of impurities can be challenging due to mobile phases not being suitable for MS analysis. In this application note, a stability indicating test for Semaglutide demonstrated efficient transition to LC conditions compatible with MS analysis using the Agilent InfinityLab multiple heart-cutting valve feature of an Agilent 1290 Infinity II bio 2D-LC. An Agilent InfinityLab LC/MSD XT exhibited the sensitivity to confirm MS spectra, even when analyzing impurities at low concentrations in the second dimension, and the deconvolution feature of Agilent OpenLab CDS software, version 2.8, facilitated easy confirmation of accurate molecular weights. Based on these results, it is anticipated that this method will provide foundational data for the management of peptide-related impurities not only for formulation compatibility but also for stability studies.
<h4>5. Thermo Fisher Scientific: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/fl_002789_ccs_dnapac_rp_columns_fl002789_en_6747763b83.pdf">Revolutionize your oligonucleotide analysis with game-changing DNAPac RP columns</a></h4>
<ul>
<li>Other</li>
</ul>
The semi-preparative format – ideal for nucleic acid purification
Product features
<ul>
<li>Designed for ion-pair reversed-phase (IP-RP) LC and LC/MS separations of both single-stranded oligonucleotides and double-stranded nucleic acids</li>
<li>Unique selectivity of the DNAPac RP chemistry, now available for puriﬁcation</li>
<li>Bioinert hardware to eliminate analyte adsorption and avoid the need for column passivation</li>
<li>Wide operating pH range and high-temperature stability (up to 110 °C)</li>
<li>High resolution and high throughput</li>
</ul>
<h4>6. Thermo Fisher Scientific: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/fl_22070_dnapac_dnaswift_oligonucleotide_fl22070_en_99e4d8f460.pdf">Thermo Scientific DNAPac columns - Ultra-high resolution oligonucleotide separation</a></h4>
<ul>
<li>Other
</li>
<li>Thermo Scientiﬁc™ DNAPac™ PA200 and DNAPac™ PA200RS Columns oﬀer the highest resolution charge based oligonucleotide separations. These anion-exchange columns provide unsurpassed resolution of full length from n–1, n+1 (and other failure sequences). 
</li>
<li>Thermo Scientiﬁc™ DNAPac™ RP (U)HPLC Columns are high-throughput, high-resolution columns designed for ion-pair reversed-phase separations. They are compatible with LC-MS, have long-lasting polymeric columns, and can operate up to pH 14. They also have temperature stability up to 110 °C. These columns can easily resolve both small polynucleotides and large dsDNA fragments up to 10,000 bp due to pores varying from 50-2000Å. Now available in semi-preparative format.
</li>
<li>Thermo Scientiﬁc™ DNASwift™ SAX-1S Monolith Column provide high loading capacity for easy laboratory scale charge-based puriﬁcation of oligonucleotide samples.
</li>
</ul>
<h4>7. Waters Corporation: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/fl_22070_dnapac_dnaswift_oligonucleotide_fl22070_en_99e4d8f460.pdf">VISUALIZATION AND QUANTIFICATION OF DRUG/METABOLITES BY SENSITIVE AND FAST TARGETED DESI IMAGING ON TQ SYSTEM</a></h4>
<ul>
<li>Poster / <a href="https://lcms.labrulez.com/events/568">ASMS 2024</a></li>
</ul>
INTRODUCTION: Mass spectrometry (MS) coupled to liquid chromatography (LC) has been typically used for drug metabolism and pharmacokinetics (DMPK) studies. However, tissues are homogenised and therefore the molecular localisation is lost.
Desorption electrospray ionization (DESI) mass spectrometry imaging (MSI) has proven to successfully map and independently quantify small molecules without radioactive labelling. Including small pharmaceutical molecules, as well as endogenous metabolites directly from tissue without the need of sample preparation after sectioning.
Tandem quadrupole (TQ) MS are renowned for their sensitivity and specificity in targeted applications using Multiple reaction monitoring (MRM) mode of acquisition.
Here, we used a commercial central nervous system (CNS) active drug named pitolisant that treats narcolepsy. It was dosed at pharmacological level and no observed adverse effect level (NOAEL) to show the distribution of the drug and its main metabolite in different organs such as liver and brain using the sensitive and fast DESI TQ system.
CONCLUSION
<ul>
<li>Pitolisant and its metabolites were detected sub 0.1 ng when spotted onto tissue section, demonstrating the high sensitivity and specificity of targeted MRM MS Imaging workflow.</li>
<li>A highly flexibility workflow is evidenced allowing high speed DESI MSI experiments with no compromise in sensitivity or including more MRM transitions to map biomarkers as well as drug.</li>
<li>Pitolisant and its oxidized metabolite were easily detected at pharmacological dose, illustrating the different distributions in the liver and brain murine tissue sections, information that wouldn’t be discovered by traditional DMPK studies.</li>
<li>MSI quantification workflow is demonstrated with the in-house MicroApp MSI Quantify.</li>
</ul>
<h4>8. Waters Corporation: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/2024_asms_claude_q_tof_ae3ec65164.pdf">EVALUATION OF DESI MOUNTED ON Q-TOF AND TQ MASS SPECTROMETERS TO IMAGE PFAS COMPOUNDS IN DIFFERENT MATRICES</a></h4>
<ul>
<li>Poster / <a href="https://lcms.labrulez.com/events/568">ASMS 2024</a></li>
</ul>
INTRODUCTION: Per- and polyfluoroalkyl substances (PFAS) are a well- known group of chemicals that have a variety of commercial and consumer uses. They have been found to be highly persistent and can accumulate in humans, animals and the environment.
Traditionally, analysis of PFAS has focused on determination with LC-MS/MS. Often laborious SPE techniques are required before analysis of solid matrices, such as food, plants or animal tissue to assess exposure routes. Furthermore, samples are homogenized, and therefore the information of where the PFAS accumulate is not available.
Here we evaluate mass spectrometry imaging (MSI) to detect PFAS directly from sample surfaces. Figure 5. Multiple conformations of N-EtFOSAA and corresponding spe- cific drift time ion images of the dilution series. Analyzed using SE- LECT SERIES Cyclic IMS MS in IMS mode . A desorption electrospray ionization (DESI) source combined with two types of Q-Tof mass spectrometers operating in full scan MS (with and without, and a high performance tandem quadrupole mass spectrometer operating in targeted multiple reaction monitoring (MRM) mode were used to evaluate limits of detection (LODs) of several PFAS compounds.
CONCLUSION
<ul>
<li>61% and 55% of the PFAS compounds were detected as low as 10 ng/mL spotted on tissue when analyzed by negative mode DESI in discovery full scan MS acquisition mode.</li>
<li>The targeted MRM acquisition mode on the DESI XS Xevo TQ Absolute MS is the most sensitive system with 77% of the PFAS compounds detected as low as 10 ng/mL spotted on tissue.</li>
<li>PFAS compounds have a different ion mobility, with faster arrival time in the gas phase, allowing differential selection and a decrease of interference signal.</li>
<li>Different conformations of several PFAS were detected by ion mobility with only a single pass in the cyclic device.</li>
<li>Distinct distributions of PFAS in the seed and root of contaminated PFAS lentils were demonstrated.</li>
</ul>
<h4>9. Metrohm: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_eis_009_download_1f476a3249.pdf">Mott-Schottky Analysis - Using EIS for semiconductor applications</a></h4>
<ul>
<li>Application</li>
</ul>
Using EIS for semiconductor applications: Semiconductors are intrinsic to modern life, but they stand to play an even greater role in the coming energy transition. Several techniques are employed in the pursuit of improved materials for energy production and storage. One particularly popular method for studying the electronic properties of potential new semiconducting materials is MottSchottky Analysis. The technique can be best understood as an extension of electrochemical impedance spectroscopy (EIS). By performing EIS at a range of different DC bias or offset potentials, the reciprocal of the square capacitance can be derived from the data and then plotted against the DC offset itself, producing a so-called Mott-Schottky Plot. From such a plot, critical parameters such as the carrier concentration, the doping profile, and the flatband potential can be conveniently extracted. This technique is popular across different fields, as it is versatile, non-destructive, and relatively fast. Compared to other options, Mott-Schottky Analysis also requires relatively little specialist equipment. This Application Note presents an example of MottSchottky measurement on a popular semiconducting material using VIONIC powered by INTELLO.
CONCLUSIONS: In this Application Note, an example Mott-Schottky measurement is shown using VIONIC powered by INTELLO. The flatband potential and doping density have been calculated using NOVA and are comparable to values for this type of sample found in the literature.

News from LabRulezLCMS Library - Week 32, 2024

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 12th August 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!
<blockquote>
<h4>👉 <a href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></h4>
</blockquote>
<blockquote>
👉 Need info about different analytical techniques? Peek into <a href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.
</blockquote>
This week we bring to you posters, applications, and other documents from Agilent Technologies, Shimadzu, Thermo Fisher Scientific, and Waters Corporation!
<h4>1. Agilent Technologies: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_amino_acid_ecd_6545xt_advancebio_5994_7506en_agilent_bbe30346d8.pdf">Identification of Amino Acid Isomers Using Electron Capture Dissociation in the Agilent 6545XT AdvanceBio LC/Q-TOF System</a></h4>
<ul>
<li>Application</li>
</ul>
Abstract: Accurately determining the amino acid sequence is crucial for understanding a protein&#39;s structure and function. However, distinguishing between leucine (Leu) and isoleucine (Ile) poses a challenge, because they are positional isomers and cannot be differentiated using traditional collision-based fragmentation techniques. Electron‑based fragmentation offers a promising solution by producing side chain fragments that can be used to distinguish between Leu/Ile and other isobaric residues such as aspartate (Asp) and isoaspartate (isoAsp). This application note demonstrates the identification of isobaric residues in peptides and intact proteins using the Agilent 6545XT AdvanceBio LC/Q-TOF system equipped with an ExD cell for electron capture dissociation. Agilent ExDViewer software enables intuitive analysis of side chain fragmentation in Q-TOF datasets, enhancing the ability to discern between isobaric amino acids.
Conclusion: This application note describes the analysis of isobaric amino acids using an Agilent 6545XT AdvanceBio Q-TOF LC/MS system equipped with an Agilent ExD cell and the Agilent ExDViewer software tool for analysis. Electron capture dissociation is a powerful fragmentation technique that provides complementary information to CID fragmentation. These methods can be applied to investigate peptide backbone and side chain fragmentation for a range of molecules, reducing ambiguity in protein sequence analysis. The sensitivity of the Q-TOF to detect ECD fragments combined with ExDViewer for fragment analysis provides an effective solution for targeted amino acid isobar identification.
<h4>2. Waters Corporation: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_amino_acid_ecd_6545xt_advancebio_5994_7506en_agilent_bbe30346d8.pdf">MODERNIZATION OF A LEGACY NORMAL PHASE METHOD ON A MODERN HPLC SYSTEM</a></h4>
<ul>
<li>Poster / <a href="https://lcms.labrulez.com/companies/303">HPLC 2024</a></li>
</ul>
INTRODUCTION
In regulated laboratories, particularly those within the pharmaceutical industry, High-Performance Liquid Chromatography (HPLC) systems play a crucial role in routine analysis. However, some challenges arise due to legacy methods that were developed during the early days of HPLC technology. These methods have not kept pace with recent advancements and modernization in the field and do not fully leverage the latest technological improvements. Advancements in column chemistry and system robustness allow for better separation and detection of analytes.
Modern HPLC systems are more robust, reliable, and capable of handling challenging analyses and can accommodate various chromatographic modes, including Normal Phase chromatography. While legacy methods persist, regulatory agencies such as the United States Pharmacopeia (USP) provide guidance on how to adapt and adjust these methods. This helps bridge the gap between legacy methods and modern HPLC systems.
In this study, we examine the modernization of a legacy normal phase method, USP Propofol Assay, for use with a HPLC system. The original method specified an older L3 column in a non-standardized column configuration (4.6 mm x 200 mm). Given these column specifications, we compared a suitable column to coupling two columns to match the specified column dimensions. We also explored the use of a column that was scaled in accordance with USP Chapter 6211 that meets the L/dp requirement.
CONCLUSION
<ul>
<li>Legacy columns, like the L3 used for column 1, has an older packing technology and is unable to provide a suitable chromatography. </li>
<li>The use of a newer packing as in BEH Columns helps provide a successful analysis</li>
<li>Coupling two columns, albeit successful, may prove to be a much more expensive option and may be introducing more opportunities for issues to arise.</li>
<li>The third column derived from the use of the columns calculator may be the more suitable route since it adheres to the USP guidance as well as not being more expensive and time consuming as the coupling of two columns.</li>
</ul>
<h4>3. Waters Corporation: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/asms_2024_mccullagh_the_utility_of_cyclic_ion_mobility_to_improve_selectivity_and_analysis_efficiency_of_environmental_pfas_contamination_and_exposure_3f331eafb6.pdf">THE UTILITY OF CYCLIC ION MOBILITY TO IMPROVE SELECTIVITY AND ANALYSIS EFFICIENCY OF ENVIRONMENTAL PFAS CONTAMINATION AND EXPOSURE</a></h4>
<ul>
<li>Poster / <a href="https://lcms.labrulez.com/companies/296">ASMS 2024</a></li>
</ul>
INTRODUCTION
Polyfluorinated alkyl substances (PFAS) exposure is a potential contributor to increased cancer occurrence in the human population. The bio-accumulative nature of PFAS enables monitoring of levels in human biofluids to help gain understanding into exposure levels and pathways. However PFAS isomeric compounds, can be challenging to efficiently separate using liquid chromatography.
Cyclic ion mobility (cIM) provides an added dimension of separation and collision cross section (CCS) values can provide a a complimentary identification descriptor. CCS values provide an additional identification point across multiple application areas. At low concentrations where fragment information may be absent, CCS and arrival time distribution (ATD) provide an additional identification point alongside retention time (tr).
To highlight confidence of the utility of CCS values, independent of ion mobility (IM) technology, when performing PFAS non-targeted screening assays, we have used an internally developed PFAS library to cross correlate PFAS CCS reproducibility. The SELECT SERIESTM CyclicTM IMS mass spectrometer (see Figure 1) has been used to perform an inter-site/intra-site comparison and compared to published drift tube (DT) ion mobility PFAS CCS values.1
Using LC-cIM-MS, branched and linear PFAS isomers have been fully resolved. Detection of individual PFAS isomers provides opportunity to correlate cancer risk with exposure to specific PFAS isomers. Human serum samples were analysed, and data generated for PFAS isomers and isobaric biomarker isomer composition.
LC-cIM-MS (cIM resolution (R)<del>65-145) analyses were performed using a SELECT SERIES Cyclic IMS mass spectrometer. PFAS standards and human serum samples were analysed, using a 22 min reversed phase separation gradient. Using the combined peak capacity of LC-cIM, enabled resolution of linear and branched isomers, whilst simultaneously achieving an increase in analysis efficiency of 75 %. The enhanced resolution has the potential to facilitate a time efficient correlation between PFAS isomeric structure, concentration, and exposure. Linear and branched PFOS isomers were IM resolved in anonymized human serum extracts, using a 5.5-minute LC gradient. Importantly chromatographic separation was achieved for PFOS and bile acid biomarkers. Using ion mobility resolution (</del>145), we report IM enhanced specificity and resolution for biomarker isomers Taurodeoxycholic, Taurochenodeoxycholic and Tauroursodeoxycholic acid.
A focus on LC-cIM-MS as a potential strategy to generate time efficient, additional toxicological correlation specificity with individual PFAS isomeric species and resultant elevation of isobaric biomarker isomers is presented. Identification of perfluoroalkyl carboxylic acids (PFCAs), cIM conformeric profiles and corresponding CCS identification finger prints provides newfound specificity.
<h4>4. Shimadzu: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/an_01_00757_en_5e7f73a1aa.pdf">Qualitative Analysis of Drug Metabolites Using LCMS-9050</a></h4>
<ul>
<li>Application</li>
</ul>
User Benefits
<ul>
<li>The LCMS-9050 quadrupole time-of-flight (Q-TOF) mass spectrometer and LabSolutions Insight Explore™provide qualitative analysis of drug metabolites.</li>
<li>Analyze and Target Screening function in LabSolutions Insight Explore enables peak extraction of candidate drug metabolites</li>
</ul>
Introduction: After a drug is ingested into the body, it is metabolized by various reactions, including oxidation, reduction, hydrolysis, and conjugation. The prediction of drug metabolites is important because the metabolites may differ from the original drug in terms of efficacy and toxicity.
This Application News introduces an example of qualitative analysis of drug metabolites using the LCMS-9050 quadrupole time-of-flight (Q-TOF) mass spectrometer. Lenvatinib, a known anticancer drug, was administered orally to rats, and the metabolites in the liver were analyzed. Drug metabolite peaks were extracted using the Analyze function in LabSolutions Insight Explore. The Target Screening function narrowed down the candidate peaks based on a list of drug metabolites, and the MS and MS/MS spectra were analyzed in detail.
Conclusion: Several metabolites derived from lenvatinib were detected by the LCMS-9050 quadrupole time-of-flight mass spectrometer and LabSolutions Insight Explore. By preparing a list of drug metabolites, it was possible to easily extract candidate metabolite peaks. This workflow can also be applied to the metabolite analysis of drugs otherthan lenvatinib.
<h4>5. Agilent Technologies: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/an_lib_electolyte_cary_3500_flexible_uv_vis_5994_7602en_agilent_ebebd9a4ad.pdf">Quality Control of Lithium-Ion Battery Electrolytes and Solvents by UV-Vis Spectroscopy</a></h4>
<ul>
<li>Application</li>
</ul>
<h5>Color measurements and safe chemical handling using an Agilent Cary 3500 Flexible UV-Vis with Cary Sipper pump</h5>
Abstract: Ensuring the quality of electrolytes used in lithium-ion batteries (LIBs) is crucial for maintaining the safety, performance, and longevity of these energy storage devices. LIB electrolytes consist of lithium salts dissolved in organic solvents. Any discoloration (yellowness) of these near-clear solutions can indicate contamination or degradation. Industry methods such as ASTM D5386-16 Standard Test Method for Color of Liquids Using Tristimulus Colorimetry can be applied to assess the quality of electrolytes by conducting instrumental color measurements using UV-Vis spectroscopy. Given the low absorbance of colorless-to-near-colorless liquid samples like LIB electrolytes and solvents, a highly accurate and sensitive UV-Vis spectrophotometer is essential.
This study demonstrates an effective quality assessment method for LIB electrolytes and solvents using the Agilent Cary 3500 Flexible UV-Vis spectrophotometer fitted with the optional Agilent Cary Sipper flow cell pump. The method is suitable for production quality control of hazardous samples, ensuring safe sample handling, high throughput, and high quality results for production-critical chemicals.
Conclusion: The Agilent Cary 3500 Flexible UV-Vis spectrophotometer provided accurate and precise color measurements for colorless to near-colorless LIB electrolytes and solvents in accordance with the ASTM D5386 standard method. Advantages of the Cary 3500 Flexible method and findings based on the data set include:
<ul>
<li>Hazardous samples were introduced directly into a 10 mm path length flow cell using the Cary Sipper pump, ensuring user safety and high sample throughput without compromising data quality.</li>
<li>The superior sensitivity (high signal-to-noise ratio) of the Cary 3500 combined with Agilent Cary WinUV Color application software enabled the reliable monitoring and control of electrolyte properties by analyzing light absorption in the visible spectrum.</li>
<li>Higher Pt-Co color (APHA) values of the used LIB electrolytes compared to fresh samples suggested a deterioration in quality due to the color changes.</li>
<li>Color measurements could be used to improve the quality control of LIB electrolytes and solvents by identifying discoloration—an indication of contamination or degradation that could affect battery performance.</li>
<li>This effective method could be implemented in both manufacturing QC settings and R&amp;D laboratories.</li>
</ul>
QC color measurements using the Cary 3500 Flexible UV-Vis would ensure that only high-quality electrolytes are used in the production of LIBs, enhancing their safety, efficiency, and durability.
<h4>6. Thermo Fisher Scientific: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/xx_002781_lc_vanquish_method_moderization_guide_tip_sheet_xx002781_en_aca7492d0b.pdf">A Helpful Guide To Modernizing Your Old HPLC Methods</a></h4>
<ul>
<li>Guide</li>
</ul>
Modernizing older or compendial methods can help increase your lab’s productivity and lower your cost and carbon footprint. This guide gives you an overview of what method parameters to consider changing, as defined by USP 621.
<h4>7. Thermo Fisher Scientific: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/wp_003007_lsms_Stellar_LCMS_translational_research_ffe410ad04.pdf">Transitioning biomarkers from discovery to validation at unprecedented scale with the Stellar mass spectrometer</a></h4>
<ul>
<li>Technical note</li>
</ul>
<h5>Expanded target capacity, higher sensitivity with greater specificity with unrivaled quantitative productivity for translational research</h5>
Keywords: Stellar mass spectrometer, biomarker verification, targeted quantitative solutions, protein, metabolite, lipid, dynamic retention time adjustment, automated method creation, fast polarity switching, translational research.
Introduction: The Thermo Scientific™ Stellar™ mass spectrometer establishes a new paradigm to drive biomarker verification by achieving 10X the sensitivity for 5X more compounds at scale compared to existing technologies. By synergistically combining the robust quantitative performance of triple quadrupole technology with the sensitive, rapid full scan MSn acquisition of dual-pressure linear ion trap technology, the Stellar mass spectrometer extends the unprecedented analytical capabilities to a wider range of compounds. Single-ion detection capabilities expand robust targeted quantitation for single cell or low protein load studies minimizing the potential for missing data. Novel software tools streamline highly multiplexed targeted quantitative method creation, implementation, and data acquisition, bypassing lengthy and costly replicate injections associated with existing technology. The new experimental capabilities of the Stellar mass spectrometer make it ideally suited for transitioning putative biomarker candidates from discovery to validation for translational proteomics, metabolomics, and lipidomics research.

News from LabRulezLCMS Library - Week 33, 2024

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News from LabRulezLCMS Library - Week 33, 2024

News from LabRulezLCMS Library - Week 32, 2024

News from LabRulezLCMS Library - Week 31, 2024

News from LabRulezLCMS Library Week 27, 2024

Gel Permeation (GPC) / Size Exclusion Chromatography (SEC) Webinars

Overview of LC/QQQ applications of pesticide analysis - Food and Environment (2020-2021)

Upcoming events

Waters ASMS Breakfast Seminars 2026

ASMS 2026: Agilent Breakfast Sessions

SCIEX at ASMS 2026

Webinars

Data handling and DMPK analysis with confidence

Reliable quantitation of GLP‑1 analogues in bioanalysis and IP 2026 updates

Enhancing Next-Gen Drug Discovery with High-Performance Inert LC Solutions